Pump Patents (Class 422/505)
  • Patent number: 10493448
    Abstract: In one arrangement, a cartridge includes a cartridge body defining a holding compartment, first and second fractioning compartments, and a number of flow channels formed within the cartridge body. A predetermined quantity of fluid can be held in the holding compartment when the cartridge body is held in a first orientation, and can be poured from the holding compartment to the first fractioning compartment by rotating the cartridge body about a predefined rotation axis to a second orientation, spilling the fluid from the holding compartment to the first fractioning compartment through one of the flow channels. The first fractioning compartment is such that when the cartridge body is in the second orientation, not all of the fluid can be contained in the first fractioning compartment, and fluid that overflows the first fractioning compartment flows through a second flow channel to the second fractioning compartment.
    Type: Grant
    Filed: March 8, 2016
    Date of Patent: December 3, 2019
    Assignee: Bio-Rad Laboratories, Inc.
    Inventors: Glenn Price, Chris Charlton, William Link, Robert Alan Iovanni, Anthony F. Prestigiacomo
  • Patent number: 10413899
    Abstract: The specification generally discloses systems and methods for mixing and delivering fluids in microfluidic systems. The fluids can contain, in some embodiments reagents that can participate in one or more chemical or biological reactions.
    Type: Grant
    Filed: July 12, 2017
    Date of Patent: September 17, 2019
    Assignee: OPKO Diagnostics, LLC
    Inventors: Enqing Tan, Vincent Linder
  • Patent number: 10350596
    Abstract: A planar micro-reagent cartridge comprises a base layer having a fluid receiving cavity formed in a top surface thereof and a fluid disposed within the cavity, and intermediate layer and a top layer. The intermediate membrane layer is bonded to the base layer to seal the fluid receiving cavity and form a fluid reservoir pump chamber. The intermediate layer comprises a resiliently deformable flexible region that overlies the fluid receiving cavity and is configured to be depressed in response to application of pressure, and a rupturable valve region that overlies the fluid receiving cavity. A top layer is bonded to the intermediate layer, the top layer having an opening that exposes the deformable flexible region of the intermediate membrane layer and at least one channel that together with the intermediate membrane layer forms a fluid conduit having an inlet in fluid communication with the rupturable valve region of the intermediate layer.
    Type: Grant
    Filed: November 14, 2013
    Date of Patent: July 16, 2019
    Assignee: Technological Uniersity Dublin
    Inventors: Brian Seddon, Ronan O'Reilly, Eithne Dempsey, James Hayes, Baljit Singh
  • Patent number: 10131903
    Abstract: This invention provides methods and compositions for assembling biological constructs (e.g., plasmids, transformed cells, etc.). In certain embodiments the methods involve encapsulating separate components of the biological construct each in a fluid droplet confined in a fluid channel; optionally mixing droplets from different fluid channels to form a sequenced order of droplets carrying different components of the biological construct in a channel or chamber; and optionally combining two or more droplets each containing different components of the biological construct to permit the components to react with each other in one or more reactions contributing to the assembly of the biological construct.
    Type: Grant
    Filed: April 2, 2012
    Date of Patent: November 20, 2018
    Assignees: The Regents of the University of California, Sandia Corporation
    Inventors: Chieh Chang, Rajiv Bharadwaj, Anup K. Singh, Aarthi Chandrasekaran, Nathan J. Hillson
  • Patent number: 9976946
    Abstract: A sample cell apparatus for use in spectroscopic determination of an analyte in a body fluid sample includes a first plate member made from an optically clear material and a second plate member made from an optically clear material and opposing the first plate member. A channel extending into a surface of the first plate member and an opposing surface of the second plate member houses a floating seal. The floating seal surrounds a fluid chamber that retains a sample of body fluid for optical measurement. The fluid chamber may be opened for flushing by separating the first plate member from the second plate member. During measurements the fluid chamber is closed to define a repeatable optical path-length therethrough by urging the first plate member against the second plate member without compressing the floating seal between the first plate member and the second plate member.
    Type: Grant
    Filed: April 21, 2016
    Date of Patent: May 22, 2018
    Assignee: Instrumentation Laboratory Company
    Inventors: Brian Joseph Bosy, Josef Kerimo
  • Patent number: 9970437
    Abstract: A delivery system for a sensor chip includes a plurality of selectable ports and a two-way pump port selectively connectable to each of the selectable ports. The two-way pump port is configured to allow material to be drawn or delivered from or to the two-way pump port. The delivery system also includes a chamber and a bypass waste channel that is selectively connectable to the two-way pump port. The plurality of selectable ports includes a selectable chamber port connected to the chamber and the chamber has a chamber waste exit. Material may selectively flow through the chamber to a waste collection via the chamber waste exit or flow to the waste collection via the bypass waste channel that bypasses the chamber waste exit.
    Type: Grant
    Filed: September 28, 2015
    Date of Patent: May 15, 2018
    Assignee: Genia Technologies, Inc.
    Inventor: Robert A. Yuan
  • Patent number: 9939429
    Abstract: A device is described including a lid, a collection portion support, and a collection portion with a surface for receiving a biological sample, the lid includes a window and a window holder, the surface defines a first plane, the collection portion includes a first, a second, and a third position markers, each position marker indicates a center point marker, the center points define a second plane that has a line of intersection with the first plane, the device has an interior volume defined partially by the collection portion support, the collection portion, the window, and the window holder, the window allows visual inspection of the position markers, the collection portion support has a fluid inlet and a fluid outlet with a fluid path from the fluid inlet to the fluid outlet via the interior volume.
    Type: Grant
    Filed: July 8, 2014
    Date of Patent: April 10, 2018
    Assignee: Roche Molecular Systems, Inc.
    Inventors: Andreas Drechsler, Edwin Oosterbroek, Emad Sarofim, Heiko Schwertner
  • Patent number: 9901921
    Abstract: A disposable, point-of-care diagnostic cartridge and method of performing an invitro diagnostic test on a specimen are provided. The cartridge includes a base having a mixing chamber is formed thereon. The mixing chamber has a mixing chamber inlet and a mixing chamber outlet. A detection chamber is formed downstream from the mixing chamber in fluid communication therewith. The mixing chamber includes a mixing reservoir bounded at least in part by a flexible upper member. The flexible upper member is expandable to accommodate an increase in volume of fluid and collapsible to dispense fluid outwardly from the mixing reservoir. The mixing chamber inlet has an inlet valve member that provides one way flow of fluid into the mixing reservoir. The mixing chamber outlet has an outlet valve member that provides one way flow of fluid outwardly from the mixing reservoir to the detection chamber.
    Type: Grant
    Filed: May 31, 2016
    Date of Patent: February 27, 2018
    Inventor: David W. Wright
  • Patent number: 9795968
    Abstract: A nucleic acid amplification and detection device includes an amplification cartridge with a plurality of reaction chambers for containing an amplification reagent and a visual detection reagent, and a plurality of optically transparent view ports for viewing inside the reaction chambers. The cartridge also includes a sample receiving port which is adapted to receive a fluid sample and fluidically connected to distribute the fluid sample to the reaction chamber, and in one embodiment, a plunger is carried by the cartridge for occluding fluidic communication to the reaction chambers. The device also includes a heating apparatus having a heating element which is activated by controller to generate heat when a trigger event is detected. The heating apparatus includes a cartridge-mounting section which positioned a cartridge in thermal communication with the heating element so that visual changes to the contents of the reaction chambers are viewable through the view ports.
    Type: Grant
    Filed: April 21, 2014
    Date of Patent: October 24, 2017
    Assignee: Lawrence Livermore National Security, LLCq
    Inventor: Lawrence Dugan
  • Patent number: 9687848
    Abstract: Microfluidic system, including methods and apparatus, for processing fluid, such as by droplet generation. In an exemplary method, a sample-containing fluid may be dispensed into a well through a sample port of a channel component. The channel component may include (a) a body having a bottom surface attached to the well, and a top surface with a microchannel formed therein, and (b) an input tube projecting into the well from the bottom surface of the body. The sample-containing fluid when dispensed may contact a bottom end of the input tube and may be retained, with assistance from gravity, out of contact with the microchannel. A pressure differential may be created that drives at least a portion of the sample-containing fluid from the well via the input tube and through the microchannel.
    Type: Grant
    Filed: August 8, 2016
    Date of Patent: June 27, 2017
    Assignee: Bio-Rad Laboratories, Inc.
    Inventors: Anthony J. Makarewicz, Jr., Luc Bousse, Stefano Schiaffino
  • Patent number: 9500577
    Abstract: A sample analyzer for aspirating and analyzing a sample in a sample tube is disclosed. The sample analyzer comprises: a fluid processing section for aspirating liquid contained in a tube with a pipette and flowing the liquid through a fluid circuit; an obtaining section for obtaining identification information for identifying type of tube to be aspirated from; and a control unit. When identifying that the tube to be aspirated from is a cleaning liquid tube, the control unit controls the fluid processing section to aspirate substantially all the cleaning liquid in the cleaning liquid tube with the pipette and use all or partial amount of the aspirated cleaning liquid to clean the fluid circuit.
    Type: Grant
    Filed: August 23, 2012
    Date of Patent: November 22, 2016
    Assignee: SYSMEX CORPORATION
    Inventors: Masaharu Shibata, Toru Mizuhashi
  • Patent number: 9452430
    Abstract: A microfluidic cartridge, configured to facilitate processing and detection of nucleic acids, comprising: a top layer comprising a set of cartridge-aligning indentations, a set of sample port-reagent port pairs, a shared fluid port, a vent region, a heating region, and a set of Detection chambers; an intermediate substrate, coupled to the top layer comprising a waste chamber; an elastomeric layer, partially situated on the intermediate substrate; and a set of fluidic pathways, each formed by at least a portion of the top layer and a portion of the elastomeric layer, wherein each fluidic pathway is fluidically coupled to a sample port-reagent port pair, the shared fluid port, and a Detection chamber, comprises a turnabout portion passing through the heating region, and is configured to be occluded upon deformation of the elastomeric layer, to transfer a waste fluid to the waste chamber, and to pass through the vent region.
    Type: Grant
    Filed: June 14, 2016
    Date of Patent: September 27, 2016
    Assignee: NeuMoDx Molecular, Inc.
    Inventors: Jeffrey Williams, Michael T. Kusner
  • Patent number: 9410127
    Abstract: A micro fluidic apparatus includes a sample chamber that divides a sample into a first fluid layer and a second fluid layer due to a centrifugal force; a first enriching unit a that receives the first fluid layer from the sample chamber, forms a first complex of a first fine particle and the first target material, and separates the first complex from the first fluid layer using a density difference; and a second enriching unit that receives the second fluid layer from the sample chamber, forms a second complex of a second fine particle and the second target material, and separates the second complex from the second fluid layer using a density difference.
    Type: Grant
    Filed: July 12, 2013
    Date of Patent: August 9, 2016
    Assignee: SAMSUNG ELECTRONICS CO., LTD.
    Inventors: Min Seok S Kim, Jong-myeon Park
  • Patent number: 9403165
    Abstract: A microfluidic cartridge, configured to facilitate processing and detection of nucleic acids, comprising: a top layer comprising a set of cartridge-aligning indentations, a set of sample port-reagent port pairs, a shared fluid port, a vent region, a heating region, and a set of Detection chambers; an intermediate substrate, coupled to the top layer comprising a waste chamber; an elastomeric layer, partially situated on the intermediate substrate; and a set of fluidic pathways, each formed by at least a portion of the top layer and a portion of the elastomeric layer, wherein each fluidic pathway is fluidically coupled to a sample port-reagent port pair, the shared fluid port, and a Detection chamber, comprises a turnabout portion passing through the heating region, and is configured to be occluded upon deformation of the elastomeric layer, to transfer a waste fluid to the waste chamber, and to pass through the vent region.
    Type: Grant
    Filed: June 30, 2015
    Date of Patent: August 2, 2016
    Assignee: NeuMoDx Molecular, Inc.
    Inventors: Jeffrey Williams, Michael T. Kusner
  • Patent number: 9310281
    Abstract: An automated sample tube blotting system having one or more rotating grippers, a supply of absorbent material, a transporter, and an absorbent material removal mechanism. Each rotating gripper has a respective holder configured to selectively mate with a respective interface on a tube strip, and the one or more rotating grippers are rotatable between a first position in which the tube strip is upright and a second position in which the tube strip is inverted. The transporter is configured to move the rotating gripper in a lateral direction from a starting location to a location above the absorbent material, and in a vertical direction towards and away from the absorbent material.
    Type: Grant
    Filed: October 22, 2012
    Date of Patent: April 12, 2016
    Assignee: Qiagen Gaithersburg, Inc.
    Inventors: Bradley Scott Thomas, Brian Austin Self, Eric Schneider, Franklin Regan
  • Patent number: 9213036
    Abstract: A microfluidic passive device and a method for determining clotting time are described, of a fluid medium such as blood, of low production cost which can therefore be disposable. When optimized to determine blood clotting time, it requires a minimal whole blood sample (<5 ?L) and it is particularly suited to INR and PT determination, which can be used to autonomously by patient without venipuncture. Monitoring and processing means to interpret the results are comprised in an external coagulometer device. A production method for the manufacture of the microfluidic device is also provided.
    Type: Grant
    Filed: December 28, 2012
    Date of Patent: December 15, 2015
    Assignee: ILINE MICROSYSTEMS, S.L.
    Inventors: Iñaki Sádaba Champetier De Ribes, Juan Antonio Peón Eguiguren
  • Patent number: 9095791
    Abstract: A liquid-chromatography module includes a microfluidic cartridge housing a microfluidic substrate with a channel for transporting fluid. The microfluidic substrate has fluidic apertures through which fluid is supplied to the channel. One side of the cartridge has nozzle openings, each aligning with a fluidic aperture in the microfluidic substrate and receiving a fluidic nozzle. A clamping assembly has a plunger that is movable into a clamped position and an end housing that defines a chamber. One wall of the end housing has a fluidic block with fluidic nozzles extending into the chamber. A second wall has a slot through which the cartridge enters the chamber. When moved into the clamped position while the cartridge is in the chamber, the plunger urges the cartridge against the fluidic block such that each fluidic nozzle enters one nozzle opening and establishes fluidic communication with one fluidic aperture in the microfluidic substrate.
    Type: Grant
    Filed: March 5, 2010
    Date of Patent: August 4, 2015
    Assignee: Waters Technologies Corporation
    Inventors: David P. Prentice, Russell L. Keene, Stanislaw Koziol, Joseph D. Michienzi, Paul E. Linderson, Gary W. Bertone
  • Patent number: 9041922
    Abstract: A system and method for mounting a section onto a substrate, the system comprising: a fluid channel including: a fluid channel inlet that receives the section, processed from a bulk embedded sample by a sample sectioning module positioned proximal the fluid channel inlet, a section-mounting region downstream of the fluid channel inlet, and a fluid channel outlet downstream of the section-mounting region; a reservoir in fluid communication with the fluid channel outlet; and a manifold, fluidly coupled to the reservoir, that delivers fluid from the reservoir to the fluid channel inlet, thereby transmitting fluid flow that drives delivery of the section from the fluid channel inlet toward the section-mounting region.
    Type: Grant
    Filed: December 17, 2014
    Date of Patent: May 26, 2015
    Assignee: Alessi Technologies, Inc.
    Inventors: Nolan Orfield, Collin Rich, Vincent Alessi
  • Patent number: 9040285
    Abstract: A sensing device including an inlet port for receiving a fluid, a measurement chamber for sensing the fluid, a fluid channel coupling the inlet port and the measurement chamber for transporting the fluid from the inlet port to the measurement chamber, and a fluid stop unit for stopping and controllably releasing the flow of fluid between the inlet port and the measurement chamber.
    Type: Grant
    Filed: December 7, 2009
    Date of Patent: May 26, 2015
    Assignee: Koninklijkle Philips N.V.
    Inventors: Albert Hendrik Jan Immink, Albert F. Gootzen, Dirk Jan Nelly Vananderoye, Petrus Johannes Wilhelmus Van Lankvelt
  • Publication number: 20150118110
    Abstract: A microfluidic device unit has a control device which includes at least one actuating unit and with a carrier for a microfluidic chip. The carrier is designed as module separate from the control device, but is connected with the same by at least one connecting line such that the actuating unit can actuate at least one function at the carrier. The carrier is provided with a receptacle for the microfluidic chip.
    Type: Application
    Filed: October 17, 2014
    Publication date: April 30, 2015
    Inventor: Thomas HAHN
  • Patent number: 9011772
    Abstract: Provided herein is a bioprocessing device, bioprocessing card, and fluidics cartridge for performing automated bioprocessing of a sample. The bioprocessing card may include a plurality of pipette tips; and at least one pump in fluid communication with the plurality of pipette tips. In some embodiments, the pumps and the pipette tips are in fluid communication through a processing channel which may be a microscale channel. Also provided herein is an automated bioprocessing device comprising: at least one bioprocessing card; at least one fluidic cartridge; and an automated control system configured to control automated bioprocessing of a sample. Further provided herein are methods of use of the device, card, and cartridge.
    Type: Grant
    Filed: May 11, 2010
    Date of Patent: April 21, 2015
    Assignee: Life Technologies Corporation
    Inventors: Lars Norderhaug, Morten Egeberg, Tommy Rivrud, Lars Nokleby, Espir Kahatt, Jamie Hogan, Kornelija Zgonc, Norman Trolson
  • Patent number: 9011796
    Abstract: A device for analysing a clinical sample comprises at least one depot chamber for receiving one or more reagents and at least one process chamber, whereas the process chamber is integrated in a first support member and the depot chamber is integrated in at least a second support member, whereas the support members are arranged in that the process chamber is connectable with the depot chamber by a relative movement of the first and second support member with respect to each other. According to the invention, the device further includes a pump element for transferring the substances inside the device from one chamber to another.
    Type: Grant
    Filed: July 10, 2009
    Date of Patent: April 21, 2015
    Assignees: Carpegen GmbH, Systec Elektronik und Software GmbH
    Inventors: Max Koltzscher, Antje Rötger, Krzysztof-Wlodzimierz Siemieniewicz, Jens Heitmann, Christoffer Mai, Klaus-Gerd Schoeler, Tilmann Wolter
  • Patent number: 9005546
    Abstract: A flow cell having a support surface, and an integrated fluid reservoir made of two foil layers arranged on the support surface and joined with each other to enclose a storage space and a transport channel. The transport channel extends from a burst point that closes the storage space to a connecting opening and can be opened by fluid flowing from the storage space. A first of the foil layers of the fluid reservoir faces away from the support surface and projects beyond a second of the foil layers of the fluid reservoir that faces the support surface, and is bonded with the support surface in a projecting region.
    Type: Grant
    Filed: December 16, 2009
    Date of Patent: April 14, 2015
    Assignee: Thinxxs Microtechnology AG
    Inventor: Lutz Weber
  • Publication number: 20150098864
    Abstract: A microfluidic chip (100) with a flow-guiding body (111) and the applications thereof in biochemistry, immunology, and molecule detection. The flow-guiding body (111) is disposed in a solution tank (101) of the microfluidic chip (100), the surface of the flow-guiding body (111) is enclosed by the antigen or antibody, and the gap between the flow-guiding body (111) and a wall (105) of the solution tank is 0 mm to 1.5 mm.
    Type: Application
    Filed: September 20, 2012
    Publication date: April 9, 2015
    Applicant: BEIJING BOHUI INNOVATION TECHNOLOGY CO., LTD.
    Inventor: Qi Yang
  • Patent number: 8992858
    Abstract: A microfluidic device comprises pumps, valves, and fluid oscillation dampers. In a device employed for sorting, an entity is flowed by the pump along a flow channel through a detection region to a junction. Based upon an identity of the entity determined in the detection region, a waste or collection valve located on opposite branches of the flow channel at the junction are actuated, thereby routing the entity to either a waste pool or a collection pool. A damper structure may be located between the pump and the junction. The damper reduces the amplitude of oscillation pressure in the flow channel due to operation of the pump, thereby lessening oscillation in velocity of the entity during sorting process. The microfluidic device may be formed in a block of elastomer material, with thin membranes of the elastomer material deflectable into the flow channel to provide pump or valve functionality.
    Type: Grant
    Filed: August 21, 2007
    Date of Patent: March 31, 2015
    Assignee: The United States of America National Institute of Health (NIH), U.S. Dept. of Health and Human Services (DHHS)
    Inventors: Hou-Pu Chou, Anne Y. Fu, Stephen R. Quake
  • Publication number: 20150087544
    Abstract: A method of flowing a fluid with a tracer in a microfluidic channel of an assay device and detecting the tracer for determining the channel location or condition of the channel.
    Type: Application
    Filed: September 6, 2014
    Publication date: March 26, 2015
    Inventors: Martin A. Putnam, Jeffrey T. Branciforte, Charles O. Stanwood
  • Patent number: 8986630
    Abstract: Provided is a gas-generating material which can generate a gas in a large amount per unit time and has high storage stability. The gas-generating material 11a according to the present invention comprises a gas-generating agent that is an azo compound or an azide compound, a tertiary amine, a photosensitizing agent and a binder resin.
    Type: Grant
    Filed: September 25, 2012
    Date of Patent: March 24, 2015
    Assignee: Sekisui Chemical Co., Ltd.
    Inventors: Yoshinori Akagi, Shigeru Nomura
  • Patent number: 8986614
    Abstract: A self-contained, fully automated, biological assay-performing apparatus includes a housing; a dispensing platform including a controllably-movable reagent dispensing system, disposed in the housing; a reagent supply component disposed in the housing; a pneumatic manifold removably disposed in the housing in a space shared by the dispensing platform, removably coupled to a fluidic transport layer and a plurality of reservoirs, wherein the fluidic transport layer, the reservoirs, and a test sample to be introduced therein are disposed in the housing in the space separate from the dispensing platform; a pneumatic supply system removably coupled to the pneumatic manifold in the housing in a space separate from the dispensing platform; and a control system coupled to at least one of the dispensing platform and the pneumatic supply system, disposed in the housing.
    Type: Grant
    Filed: March 15, 2013
    Date of Patent: March 24, 2015
    Assignee: Rheonix, Inc.
    Inventors: Peng Zhou, Lincoln C. Young, Benjamin Thomas, Zongyuan Chen, Todd Roswech, Gwendolyn Spizz, Rubina Yasmin, Greg Mouchka
  • Patent number: 8986983
    Abstract: Cassette (50) performs assays, e.g. multiplexed protein biomarker assays. Wide, bubble-free, slow flows are produced from liquids stored on cassette (50), flowing over wide array (20) of ligand receptors on a capture surface. Flows of Reynolds Number less than about 1, preferably 1×10?1 to 5×10?3, are heated in region (34) preceding and including bubble removal system (128). Analyte is introduced through compressed septum (32). External actuations of displacement pumps (30, 37) and valves (137 A, B, and C) produce flows in response to flow-front optical sensors (150, 152). Elastic sheet provides pump and valve diaphragms and resilient expansion of mixing volume (131). Break-away cover portions are pistons. Heating is by conduction through cassette from external contact heater. Planar cassette body, when tilted from horizontal, enables upward flow from pumped storage (134, 135) to reaction (133) to waste (139), with buoyancy bubble removal before reaction.
    Type: Grant
    Filed: September 7, 2010
    Date of Patent: March 24, 2015
    Assignee: Courtagen Life Sciences, Inc.
    Inventors: Jean I. Montagu, Herman Deweerd, Roger Dowd, Natalia Rodionova, Peter Maimonis, Nathan Tyburczy
  • Patent number: 8980199
    Abstract: Embodiments described herein provide micro-fluidic systems and devices for use in performing various diagnostic and analytical tests. According to one embodiment, the micro-fluidic device includes a sample chamber for receiving a sample, and a reaction chamber for performing a chemical reaction. A bubble jet pump is structured on the device to control delivery of a fluid from the sample chamber to the reaction chamber. The pump is fluidically coupled to one or more chambers of the device using a fluidic channel such as a capillary. A valve may be coupled to one or more chambers to control flow into and out of those chambers. Also, a sensor may be positioned in one or more of the chambers, such as the reactant chamber, for sensing a property of the fluid within the chamber as well as the presence of a chemical within the chamber.
    Type: Grant
    Filed: March 25, 2014
    Date of Patent: March 17, 2015
    Assignee: InCube Labs, LLC
    Inventor: Mir Imran
  • Publication number: 20150073061
    Abstract: We describe a method of layer-by-layer deposition of a plurality of layers of material onto the wall or walls of a channel of a microfluidic device, the method comprising: loading a tube with a series of segments of solution, a said segment of solution bearing a material to be deposited; coupling said tube to said microfluidic device; and injecting said segments of solution into said microfluidic device such that said segments of solution pass, in turn, through said channel depositing successive layers of material to perform said layer-by-layer deposition onto said wall or walls of said channel. Embodiments of the methods are particularly useful for automated surface modification of plastic, for example PDMS (Poly(dimethylsiloxane)), microchannels. We also describe methods and apparatus for forming double-emulsions.
    Type: Application
    Filed: September 15, 2014
    Publication date: March 12, 2015
    Applicant: Cambridge Enterprise Limited
    Inventors: Wolfgang Andreas BAUER, Wilhelm T.S. HUCK, Martin FISCHLECHNER
  • Patent number: 8961904
    Abstract: A microfluidic chip orients and isolates components in a sample fluid mixture by two-step focusing, where sheath fluids compress the sample fluid mixture in a sample input channel in one direction, such that the sample fluid mixture becomes a narrower stream bounded by the sheath fluids, and by having the sheath fluids compress the sample fluid mixture in a second direction further downstream, such that the components are compressed and oriented in a selected direction to pass through an interrogation chamber in single file formation for identification and separation by various methods. The isolation mechanism utilizes external, stacked piezoelectric actuator assemblies disposed on a microfluidic chip holder, or piezoelectric actuator assemblies on-chip, so that the actuator assemblies are triggered by an electronic signal to actuate jet chambers on either side of the sample input channel, to jet selected components in the sample input channel into one of the output channels.
    Type: Grant
    Filed: July 16, 2013
    Date of Patent: February 24, 2015
    Assignee: Premium Genetics (UK) Ltd.
    Inventors: Zheng Xia, Yu Zhou, John Larsen, Guocheng Shao, Shane Peterson, Marjorie Faust
  • Publication number: 20150050719
    Abstract: A dispensing assembly comprising a cartridge holder is presented. The cartridge holder can receive a cartridge for dispensing a fluid. The cartridge can comprise a reservoir for receiving the fluid. The reservoir can comprise an outlet. The reservoir can have an adjustable volume for forcing the fluid through the outlet. The cartridge can further comprise a nozzle for dispensing the fluid. The nozzle can be connected to the outlet. The dispensing assembly can further comprise an actuator for actuating the adjustable volume. The dispensing assembly can further comprise an impulse generator for imparting an impulse to the nozzle. The impulse generator can comprise an actor for contacting the nozzle. The dispenser assembly can further comprise a controller for controlling the actor and the impulse generator.
    Type: Application
    Filed: November 3, 2014
    Publication date: February 19, 2015
    Inventors: Stefan Borja Bammesberger, Andreas Ernst, Peter Koltay, Nadine Losleben, Laurent Tanguy
  • Publication number: 20150045234
    Abstract: A fluidic system that includes a reagent manifold comprising a plurality of channels configured for fluid communication between a reagent cartridge and an inlet of a flow cell; a plurality of reagent sippers extending downward from ports in the manifold, each of the reagent sippers configured to be placed into a reagent reservoir in a reagent cartridge so that liquid reagent can be drawn from the reagent reservoir into the sipper; at least one valve configured to mediate fluid communication between the reservoirs and the inlet of the flow cell. The reagent manifold can also include cache reservoirs for reagent re-use.
    Type: Application
    Filed: August 7, 2014
    Publication date: February 12, 2015
    Applicant: ILLUMINA, INC.
    Inventors: Michael Stone, Drew Verkade
  • Publication number: 20150044758
    Abstract: The present invention provides systems, devices, apparatuses and methods for automated bioprocessing. Examples of protocols and bioprocessing procedures suitable for the present invention include but are not limited to immunoprecipitation, chromatin immunoprecipitation, recombinant protein isolation, nucleic acid separation and isolation, protein labeling, separation and isolation, cell separation and isolation, food safety analysis and automatic bead based separation. In some embodiments, the invention provides automated systems, automated devices, automated cartridges and automated methods of western blot processing.
    Type: Application
    Filed: August 19, 2014
    Publication date: February 12, 2015
    Inventors: Joseph Amshey, Daniel Bezdek, Espir Kahatt, Alexander Khorlin, Lance Parsons, Todd Peterson, Timothy Powers, Michael Thacker, Timothy Updyke, Kornelija Zgonc
  • Publication number: 20150044696
    Abstract: An integrated microfluidic device for carrying out a series of fluidic operations includes a housing including a plurality of n microfluidic conduits, wherein n is at least three, and a rotating valve having an internal channel with an entrance port and an exit port that are angularly separated. The rotating valve is positionable in a first position to connect two of the n fluidic conduits via the internal channel, and upon rotating the valve to a second position, two other of the n fluidic conduits are connected by the internal channel. The device further may include one or more fluidic chambers in fluid communication with respective fluidic conduits. Fluid contained in one fluidic chamber is transferrable by application of positive or negative gas pressure through associated fluidic conduits into another fluidic chamber via the internal channel. The device may be utilized to perform a variety of fluidic operations.
    Type: Application
    Filed: August 9, 2013
    Publication date: February 12, 2015
    Applicant: SHARP KABUSHIKI KAISHA
    Inventors: Pamela Ann DOTHIE, Daniel Christopher SPENCER
  • Publication number: 20150033872
    Abstract: The present invention provides a microfluidic device which includes at least 3 chambers, a chamber inlet, at least 2 dichotomously branching generations of channels, a channel inlet, and a channel outlet, wherein the channels and the chambers are separated by deformable walls, wherein each wall is lined with at least one cavity, and wherein the cavity is fluidly connected to the channel.
    Type: Application
    Filed: August 1, 2014
    Publication date: February 5, 2015
    Inventors: Rami Fishler, Josue Sznitman
  • Patent number: 8945481
    Abstract: Exemplary embodiments provide microfludic devices and methods for their use. The microfluidic device can include an array of M×N reaction sites formed by intersecting a first and second plurality of fluid channels of a flow layer. The flow layer can have a matrix design and/or a blind channel design to analyze a large number of samples under a limited number of conditions. The microfluidic device can also include a control layer including a valve system for regulating solution flow through fluid channels. In addition, by aligning the control layer with the fluid channels, the detection of the microfluidic devices, e.g., optical signal collection, can be improved by piping lights to/from the reaction sites. In an exemplary embodiment, guard channels can be included in the microfluidic device for thermal cycling and/or reducing evaporation from the reaction sites.
    Type: Grant
    Filed: June 24, 2008
    Date of Patent: February 3, 2015
    Assignee: Applied Biosystems, LLC
    Inventors: Mark F. Oldham, Kenneth J. Livak, Jason E. Babcoke, H. Pin Kao, Stephen J. Gunstream, Kevin S. Bodner, Douglas P. Greiner, Nigel P. Beard, Dar Bahatt
  • Publication number: 20150031116
    Abstract: The invention relates to a microfabricated device for the rapid detection of DNA, proteins or other molecules associated with a particular disease. The devices and methods of the invention can be used for the simultaneous diagnosis of multiple diseases by detecting molecules (e.g. amounts of molecules), such as polynucleotides (e.g., DNA) or proteins (e.g., antibodies), by measuring the signal of a detectable reporter associated with hybridized polynucleotides or antigen/antibody complex. In the microfabricated device according to the invention, detection of the presence of molecules (i.e., polynucleotides, proteins, or antigen/antibody complexes) are correlated to a hybridization signal from an optically-detectable (e.g. fluorescent) reporter associated with the bound molecules. These hybridization signals can be detected by any suitable means, for example optical, and can be stored for example in a computer as a representation of the presence of a particular gene.
    Type: Application
    Filed: March 5, 2014
    Publication date: January 29, 2015
    Applicant: CALIFORNIA INSTITUTE OF TECHNOLOGY
    Inventors: Stephen R. Quake, Hou-Pu Chou
  • Patent number: 8940250
    Abstract: The present invention relates to a method of continuously conveying a liquid which is used as starting material in a chemical reaction by means of a displacement pump having physically separate forward-transport valves and a liquid-filled bidirectional flow line between displacement pump and forward-transport valves, wherein an auxiliary liquid which is a product or a starting material of the chemical reaction and has a melting point which is below the melting point or below the saturation temperature of the liquid to be conveyed is present in the bidirectional flow line. The present invention additionally provides for the use of a product formed by hydrogenation of an aromatic compound as auxiliary liquid for conveying an aromatic compound and also the use of an alcohol or an ester derived from alcohol and carboxylic acid as auxiliary liquid for conveying carboxylic acids or carboxylic acid derivatives.
    Type: Grant
    Filed: July 6, 2010
    Date of Patent: January 27, 2015
    Assignee: BASF SE
    Inventors: Joachim Pfeffinger, Willi Gilcher, Juergen Morell
  • Patent number: 8940249
    Abstract: A system for the automated analysis of liquid samples having one or more processing units for reaction between the samples and one or more reagents to thereby obtain reaction products is disclosed. Disclosed also are a sample unit for supplying the samples to the one or more processing units; a reagent unit equipped with plural reagent vessels containing one or more reagents for mixing with the samples; a distribution unit for distributing fluids including the one or more reagents provided with plural distribution lines, at least some of which are connected to the reagent vessels and the one or more processing units; and at least one analytical unit for analyzing the samples based on the reaction products, in which the analytical unit may include at least one detector for detecting the reaction products.
    Type: Grant
    Filed: August 5, 2010
    Date of Patent: January 27, 2015
    Assignee: Roche Diagnostics Operations, Inc.
    Inventors: Rainer D. Jaeggi, Oliver Gutmann, Michael Glauser, Heinz-Michael Hein, Edwin Oosterbroek, Michael Andreas Heinrich
  • Patent number: 8921121
    Abstract: A chemiluminescence-based detection system and method for counting blood cells by capturing and isolating target blood cells flowing through a microfluidic chip and detecting light emitted by the captured target blood cells.
    Type: Grant
    Filed: August 20, 2010
    Date of Patent: December 30, 2014
    Assignee: The Trustees of Columbia University in the City of New York
    Inventors: Zuankai Wang, Sau Yin Chin, Samuel K. Sia
  • Publication number: 20140377146
    Abstract: A pneumatically driven portable assay cartridge having analyte capture regions associated with microfluidic channels within its interior, the portable cartridge having pneumatic ports clampable against pneumatic ports of an operating instrument for controlled application of positive pressure and vacuum to pneumatic operating channels within the cartridge, the cartridge having a well for receiving sample from a user and microfluidic channels that include pneumatically operated pistons and valves controllable by the pneumatic operating channels to cause all flows of the assay from the reservoirs through reaction regions within the cartridge to on-board waste reservoir during conduct of the assay.
    Type: Application
    Filed: September 6, 2014
    Publication date: December 25, 2014
    Inventors: Martin A. Putnam, Jeffrey T. Branciforte, Charles O. Stanwood
  • Publication number: 20140374238
    Abstract: A precision volumetric liquid dispensing instrument is disclosed that includes two pressure sensors and a fluid passageway with a defined volume portion in communication with the two sensors for receiving and distributing liquid in relatively small volumes. One of the pressure sensors is positioned to measure pressure at one portion of the defined volume portion of the fluid passageway and the other of the gas pressure sensors is positioned to measure gas pressure at a different portion of the defined volume portion of the passageway. At least one valve is in communication with the passageway for moving fluids into or out of the defined volume portion of the fluid passageway, and a processor carries out a step selected from the group consisting of (i) calculating the volume of the liquid based upon the measured pressure and (ii) metering a liquid into the defined volume portion of the fluid passageway until the measured pressure indicates that a desired volume of fluid is in the fluid passageway.
    Type: Application
    Filed: June 20, 2013
    Publication date: December 25, 2014
    Inventors: David L. Herman, Joseph J. Lambert
  • Publication number: 20140377145
    Abstract: A microfluidic mixing device comprises a main channel and a number of secondary channels extending from a portion of the main channel and entering another portion of the main channel. A number of actuators are located in the secondary channels to pump fluids through the secondary channels. A microfluidic mixing system comprises a microfluidic mixing device. The microfluidic mixing device comprises a main fluid mixing channel, a number of main channel actuators to pump fluid through the main fluid mixing channel, a number of secondary channels fluidly coupled to the main fluid mixing channel, and a number of secondary channel actuators to pump fluids through the secondary channels. The microfluidic mixing device also comprises a fluid source, and a control device to provide fluids from the fluid source to the microfluidic mixing device and activate the main channel actuators and secondary channel actuators.
    Type: Application
    Filed: June 24, 2013
    Publication date: December 25, 2014
    Inventors: Alexander Govyadinov, Pavel Kornilovich, Erik D. Torniainen, David P. Markel
  • Publication number: 20140370581
    Abstract: The present invention relates to microfluidic devices and methods for manipulating and analyzing fluid samples. The disclosed microfluidic devices utilize a plurality of microfluidic channels, inlets, valves, filter, pumps, liquid barriers and other elements arranged in various configurations to manipulate the flow of a fluid sample in order to prepare such sample for analysis.
    Type: Application
    Filed: February 14, 2014
    Publication date: December 18, 2014
    Applicant: Micronics, Inc.
    Inventors: Patrick Saltsman, Mingchao Shen, Jeffrey M. Houkal, Christy A. Lancaster, C. Frederick Battrell, Bernhard H. Weigl
  • Patent number: 8900530
    Abstract: The present invention relates to a micro-volume liquid ejection system, including an air pressure module, a micro-ejection unit which is connected with the air pressure module by a conduit, and a control circuit which is connected with the air pressure module and the micro-ejection unit respectively. The air is used as the pressure medium, resulting in improved cleaning process and reduced sample waste. The present invention includes an electric control circuit to pick up sample, eject sample and clean the conduits automatically, and enables handling of a multiplicity of samples. The present invention can be used for transferring or dispensing micro volume liquid including biological liquid on a nL and ?L scale.
    Type: Grant
    Filed: November 22, 2004
    Date of Patent: December 2, 2014
    Assignees: CapitalBio Corporation, Tsinghua University
    Inventors: Dong Wang, Kun Zou, Jianxing Ye, Yelei Sun, Hui Zhu, Xianhua Wang, Jing Cheng
  • Patent number: 8900877
    Abstract: A parallel processing system for processing samples is described. In one embodiment, the parallel processing system includes an instrument interface parallel controller to control a tray motor driving system, a close-loop heater control and detection system, a magnetic particle transfer system, a reagent release system, a reagent pre-mix pumping system and a wash buffer pumping system.
    Type: Grant
    Filed: April 30, 2012
    Date of Patent: December 2, 2014
    Assignee: Luminex Corporation
    Inventors: Steve Jia Chang Yu, Jesus Ching, Phillip You Fai Lee, David Hsiang Hu
  • Patent number: 8895312
    Abstract: A microfluidic device for mitochondria analysis includes an inlet coupled to a first access channel, an outlet coupled to a second access channel, and a plurality of trapping channels fluidically coupled at one end to the first access channel and fluidically coupled at an opposing end to the second access channel, each trapping channel has a cross-sectional dimension about 2 ?m in one direction and a cross-sectional dimension between about 0.45 and about 0.75 ?m in a second direction.
    Type: Grant
    Filed: August 9, 2013
    Date of Patent: November 25, 2014
    Assignee: The Regents of the University of California
    Inventors: Peter Burke, Katayoun Zand, Ted Pham, Antonio Davila, Douglas Wallace
  • Patent number: 8894947
    Abstract: A microfluidic processing device includes a substrate defining a microfluidic network. The substrate is in thermal communication with a plurality of N independently controllable components and a plurality of input output contacts for connecting the substrate to an external controller. Each component has at least two terminals. Each terminal is in electrical communication with at least one contact. The number of contacts required to independently control the N components is substantially less than the total number of terminals. Upon actuation, the components typically heat a portion of the microfluidic network and/or sense a temperature thereof.
    Type: Grant
    Filed: March 19, 2013
    Date of Patent: November 25, 2014
    Assignee: HandyLab, Inc.
    Inventors: Karthik Ganesan, Kalyan Handique