Liquid Moving Means Patents (Class 422/504)
  • Patent number: 10732649
    Abstract: The present invention provides microfluidic technology enabling rapid and economical manipulation of reactions on the femtoliter to microliter scale.
    Type: Grant
    Filed: June 30, 2017
    Date of Patent: August 4, 2020
    Assignee: The University of Chicago
    Inventors: Rustem F. Ismagilov, Bo Zheng, Cory John Gerdts
  • Patent number: 10732177
    Abstract: Compositions, methods, and devices for the detection of multiple analytes with a single signal are provided.
    Type: Grant
    Filed: November 1, 2017
    Date of Patent: August 4, 2020
    Assignee: INVISIBLE SENTINEL, INC.
    Inventors: Nicholas Siciliano, Louis Leong, Martin Patrick Keough, Ashley Shaniece Brown
  • Patent number: 10725541
    Abstract: Systems and methods for forming a fluidic device using a layered construction strategy may include (1) forming a first component for the fluidic device out of a first material, (2) forming a second component for the fluidic device out of a second material that is different than the first material, and (3) after forming the first and second components, forming the fluidic device by assembling a variety of components including the first component and the second component.
    Type: Grant
    Filed: May 31, 2018
    Date of Patent: July 28, 2020
    Assignee: Facebook Technologies, LLC
    Inventors: Riccardo Desalvo, Matthew Schwab, Joseph Minh Tien, Jack Lindsay, Serol Turkyilmaz
  • Patent number: 10718759
    Abstract: Disclosed herein are a transparent immunoassay apparatus and method. The transparent immunoassay apparatus includes a sample pad configured to allow a liquid specimen to be dropped thereonto, a first porous pipe configured to move the dropped specimen in a lateral flow manner, an immunobinding reaction unit configured to generate a detectable signal from binding of a previously applied reactant and the specimen between transparent upper and lower plates of the immunobinding reaction unit, a second porous pipe configured to move the specimen, having passed through the immunobinding reaction unit, in a lateral flow manner, and an absorption pad configured to absorb the specimen transferred from the second porous pipe.
    Type: Grant
    Filed: June 5, 2017
    Date of Patent: July 21, 2020
    Assignee: ELECTRONICS AND TELECOMMUNICATIONS RESEARCH INSTITUTE
    Inventors: Yo-Han Choi, Ki-Bong Song
  • Patent number: 10705012
    Abstract: The invention is related to a device and method for analysing and controlling cell motility. In accordance with an aspect of the present invention, there if provided a microfluidic device for analyzing and controlling the motility of a cell, the device comprising: (a) a first inlet for introducing a cell sample; (b) an outlet for discharging the cell sample; (c) a microfluidic channel in fluid communication with and intermediate the first inlet and outlet; (d) a first pump coupled to the first inlet for pumping the cell sample in the microfluidic channel; and (d) an observation area within a portion of the microfluidic channel for analysing and controlling the motility of the cell, wherein the first pump generates a shear stress in the observation area, the shear stress generates a shearotactical signal for driving movement of the cell.
    Type: Grant
    Filed: April 22, 2016
    Date of Patent: July 7, 2020
    Inventors: Roland Bouffanais, Xiaoying Zhu, Dick K. P. Yue
  • Patent number: 10688493
    Abstract: A device for performing a microfluidic assay on a chip comprising, a microfluidics chip, one or more fluid receptacles on the chip for receiving a fluid, a plurality of pneumatic pumps arrayed on the chip, each pump having a discharge channel leading to a rectifier on the chip, and a reaction chamber in fluid communication with each of the rectifiers, wherein a pressure on the pressurized fluid source drives fluid from the fluid receptacle into the incoming fluid channel connecting the fluid receptacle to the pump, through the pump and into the discharge channel, through the discharge channel to the rectifier, and through the rectifier into the reaction chamber, wherein the pump is configured to generate droplets of a pre-determined size, wherein the rectifiers prevent backflow of the droplets, and wherein droplets are combined in the reaction chamber, the chamber facilitating an assay being performed on the chip.
    Type: Grant
    Filed: March 9, 2017
    Date of Patent: June 23, 2020
    Assignee: Texas Tech University System
    Inventors: Jungkyu Kim, Vladimir Coltisor
  • Patent number: 10683477
    Abstract: The present invention provides a device for producing a large number of uniform spheroids by an easy method. The spheroid-producing device (1) at least includes a first surface (11), a second surface (12), and a plurality of wall surfaces (13). The second surface (12) faces the first surface (11). The respective wall surfaces (13) constitute a plurality of holes penetrating through the first surface and the second surface. In addition, an equivalent diameter of inscribed circles of openings in the first surface (11) is greater than an equivalent diameter of inscribed circles of openings in the second surface (12).
    Type: Grant
    Filed: February 25, 2015
    Date of Patent: June 16, 2020
    Assignee: Corning Incorporated
    Inventor: Shoichiro Sumi
  • Patent number: 10646865
    Abstract: Sample manipulating tools may comprise a handle portion and a tool portion. A sample spreading tool may comprise a head connected to the handle portion by a pivot connection permitting at least pivotal movement of the tool portion with respect to the handle portion. The head may comprise a perimeter section and a bridge section. Another sample spreading tool may comprise a central element and at least two loop segments connected to the central element with each loop segment having a substantially arcuate shape. A sample picking tool may have a loop section forming a reservoir for receiving a liquid sample and a hook portion fixed to the loop section, with a first end fixed to the loop portion and a second end being a free end. A sample scraping tool may comprise a mounting structure to receive a pipette and a scraper structure having a scraping edge.
    Type: Grant
    Filed: March 7, 2018
    Date of Patent: May 12, 2020
    Inventor: John L. Sternick
  • Patent number: 10634672
    Abstract: A device is provided. The device comprises a casing comprising an interior, a first opening, and a second opening; and a porous carrier comprising a sample-receiving zone and a target cell-binding zone. The porous carrier defines a first fluid pathway that extends from the sample-receiving zone to the target cell-binding zone. At least portion of the porous carrier is disposed in the interior of the casing. A second fluid pathway comprising a central axis extends through the casing from the first opening and the second opening, the second fluid pathway intersecting the porous carrier at the target cell-binding zone. The central axis is oriented orthogonally with respect to the porous carrier. Methods of using the device to detect a target microorganism are also provided.
    Type: Grant
    Filed: October 5, 2015
    Date of Patent: April 28, 2020
    Assignee: 3M INNOVATIVE PROPERTIES COMPANY
    Inventors: Neil Percy, Gregory W. Sitton
  • Patent number: 10618047
    Abstract: This disclosure relates to an apparatus for simultaneously filling a plurality of sample chambers. In one aspect, the apparatus comprises a common fluid source and a plurality of independent, continuous fluidic pathways. Each independent, continuous fluidic pathway comprises a sample chamber and a pneumatic compartment. The sample chamber is connected to the common fluid source, and the pneumatic compartment is connected to the sample chamber. The sample chamber comprises, in part, an assay chamber. The assay chamber comprises a monolithic substrate and a plug. In some embodiments, the assay chamber contains a magnetic mixing element. In some embodiments, the assay chamber is a double tapered chamber. In some embodiments, a ratio of a volume of the sample chamber to a volume of the pneumatic compartment is substantially equivalent for each fluidic pathway of the plurality of fluidic pathways.
    Type: Grant
    Filed: July 5, 2018
    Date of Patent: April 14, 2020
    Assignee: Talis Biomedical Corporation
    Inventor: Thomas H. Cauley, III
  • Patent number: 10598668
    Abstract: Coating materials such as MALDI matrix solutions are aerosolized and are used to coat analyte particles in an acoustic coater. Methods and devices for coating analyte particles in real time are disclosed. The coating improves the detection and quantification of the analyte particles using analytical instruments such as an aerosol time of flight mass spectrometer.
    Type: Grant
    Filed: August 24, 2016
    Date of Patent: March 24, 2020
    Assignee: Zeteo Tech, Inc.
    Inventors: Wayne A. Bryden, Charles J. Call
  • Patent number: 10564087
    Abstract: Provided are methods for quantifying and/or detecting sub-visible particulates in cell cultures. Specifically, the methods comprise a step of breaking down, e.g., lysing, cells in a cell culture. The methods can further comprising filtering the cell culture through a filter. Further provided are methods of quantifying sub-visible particulates that do not pass through the filter using a microscope.
    Type: Grant
    Filed: September 11, 2015
    Date of Patent: February 18, 2020
    Assignee: Celularity, Inc.
    Inventors: Lauren M. Depalma, Daniel Dewitt, Li Ren
  • Patent number: 10564121
    Abstract: Disclosed herein is a device for separation and analysis of trace and ultra-trace ionogenic compounds by isotachophoresis-zone electrophoresis on a chip with online detection and method of its use, concentrating trace and ultra-trace analytes by isotachophoretic migration in a wide separation channel when a manifold of auxiliary electrodes is employed. Then the isotachophoretic zones of trace and ultra-trace analytes are transferred by isotachophoretic migration through a tapered channel, while corresponding auxiliary electrodes are progressively disconnected from the power supply. When the isotachophoretic zones enter the analytical capillary channel, the mode switches to zone electrophoresis and an online detector detects analytes for qualitative and quantitative analysis.
    Type: Grant
    Filed: November 26, 2015
    Date of Patent: February 18, 2020
    Inventor: Vladislav Dolnik
  • Patent number: 10564171
    Abstract: A microfluidic diagnostic chip may comprise a substrate, a plurality of fluidic slots extending through the substrate, a plurality of microfluidic channels each coupled to a respective one of the plurality of fluidic slots to receive a plurality of fluids from the plurality of fluidic slots, and a mixing region in fluid communication with the plurality of fluidic slots to receive the plurality of fluids such that the plurality of fluids are to mix. A diagnostic chip may comprise a number of fluid slots defined through a substrate and a plurality of microfluidic channels coupled to the fluid slots to receive from the fluid slots a plurality of different fluids wherein the microfluidic channels combine and mix the plurality of different fluids.
    Type: Grant
    Filed: January 30, 2015
    Date of Patent: February 18, 2020
    Assignee: Hewlett-Packard Development Company, L.P.
    Inventors: Nicholas Matthew Cooper McGuinness, Chantelle Elizabeth Domingue, Manish Giri, Ed Friesen
  • Patent number: 10527171
    Abstract: Disclosed herein are methods, devices, and systems for fluidic handling. In some embodiments, a gasket for providing a fluidic interface with a flowcell includes an inner cavity extending distally from a proximal end of the gasket, the inner cavity being defined by a plurality of inner surfaces sections, an inlet port positioned at a distal end of the inner cavity, an outlet port positioned at a distal end of the gasket, and a cannula extending between the inlet port and the outlet port; wherein at least some of the plurality of inner surface sections are tapered towards the distal end of the gasket to direct a pipette tip received within the gasket towards the inlet port of the gasket.
    Type: Grant
    Filed: August 15, 2017
    Date of Patent: January 7, 2020
    Assignee: Cellular Research, Inc.
    Inventors: Philipp S. Spuhler, Geoffrey R. Facer, Sixing Li, Christopher G. Cesar
  • Patent number: 10527554
    Abstract: This invention is in the field of medical devices. Specifically, the present invention provides fluidic systems having a plurality of reaction sites surrounded by optical barriers to reduce the amount of optical cross-talk between signals detected from various reaction sites. The invention also provides a method of manufacturing fluidic systems and methods of using the systems.
    Type: Grant
    Filed: August 29, 2017
    Date of Patent: January 7, 2020
    Assignee: Theranos IP Company, LLC
    Inventors: Timothy Michael Kemp, Chris Todd, Ron Oral, Shulin Zeng, John Howard, Jeff Fenton, Elizabeth A. Holmes, Ian Gibbons, Shaunak Roy
  • Patent number: 10527595
    Abstract: Provided are a method of and an apparatus for formulating a multicomponent drug capable of surely making a multicomponent drug meeting criteria for productization with high accuracy into a product. The method and apparatus obtain a chromatogram from an extract or a base of a multicomponent drug, evaluate whether the base meets the criteria for productization based on the obtained chromatogram with high accuracy, and subject the base determined in the high-accuracy evaluating as an accepted one meeting the criteria to dosage form processing, to produce a formulated drug having a given dosage-form.
    Type: Grant
    Filed: September 6, 2016
    Date of Patent: January 7, 2020
    Assignee: Tsumura & Co.
    Inventors: Yoshikazu Mori, Keiichi Noda
  • Patent number: 10527611
    Abstract: The present invention provides a device for isolating target biomolecules or cells from samples, particularly biological samples. In particular, the device comprises a loading mixture, which contains the biological sample and a first binding entity that specifically binds to the target biomolecule or target cell; and a micro-channel coated with a second binding entity that binds directly or indirectly to the first binding entity. Methods of capturing, detecting, and/or evaluating target biomolecules or target cells (e.g. cancer cells) in biological samples are also disclosed.
    Type: Grant
    Filed: July 29, 2015
    Date of Patent: January 7, 2020
    Assignee: Biocept, Inc.
    Inventors: Stephen Mikolajczyk, Tony Pircher, Pavel Tsinberg, Farideh Z. Bischoff
  • Patent number: 10500588
    Abstract: According to the invention, generally, a microfluidic aliquot (MA) chip, adapted to fit in a Petri dish, has a center well (inlet) connected by branched channels to a plurality of side wells (outlets). The chip comes in various types, including a bMA Chip T1, bMA Chip T2, bMA Chip T3, and an rMA Chip. The branched channel improvement provides for a greater distance between neighboring channels and a decreased density near the center well. An insert and a base are configured to create an MA chip.
    Type: Grant
    Filed: November 24, 2017
    Date of Patent: December 10, 2019
    Inventor: Lidong Qin
  • Patent number: 10493444
    Abstract: A pipetting device (100) for an apparatus for processing a sample or reagent is disclosed. The pipetting device (100) comprises a coupling mechanism, wherein the coupling mechanism comprises at least a first coupling unit (104) adapted to be coupled to a first pipetting tip (108) and a second coupling unit (106) adapted to be coupled to a second pipetting tip (110), and a selection element (138) for selectively allowing a coupling of the first coupling unit (104) to or releasing the first coupling unit (104) from the first pipetting tip (108) and for selectively allowing a coupling of the second coupling unit to or releasing the second coupling unit (106) from the second pipetting tip (110). The selection element (138) is mechanically coupled to the first coupling unit (104) and the second coupling unit (106). Further, an apparatus (198) for processing a sample or reagent and a method for pipetting a sample or reagent are disclosed.
    Type: Grant
    Filed: October 12, 2016
    Date of Patent: December 3, 2019
    Assignee: Roche Molecular Systems, Inc.
    Inventors: Hans-Rudolf Bachmann, Rolf Schneebeli, Geza Burghardt, Kurt Barmettler
  • Patent number: 10463868
    Abstract: A device for treating a body surface of a living body by means of a dielectric barrier discharge plasma, with a planar, flexible dielectric (4) by which an electrode (1) attached to a high voltage source (3) is screened off from the body surface and which is suitable for placing on the body surface, functioning as counter-electrode, and is designed with a structured surface (7) which provides a gas space for the plasma discharge between dielectric (4) and body surface, which device permits the conduct of plasma treatment and the delivery of curative or healing substances without a change of the position of the device, by virtue of the fact that a layer (11) composed of a solid, open-pore matrix of a curative or healing material is arranged on the surface (7) of the dielectric (4).
    Type: Grant
    Filed: November 6, 2014
    Date of Patent: November 5, 2019
    Assignee: CINOGY GMBH
    Inventors: Dirk Wandke, Leonhard Trutwig, Karl-Otto Storck, Mirko Hahnl, Frank Pallaske, Steffen Haertlein, Georg Daeschlein
  • Patent number: 10451590
    Abstract: Disclosed sensors can include at least one resonator (in some embodiments, at least two resonators) and various other structures that may be formed in association with the resonators. The at least one resonator in embodiments can include a bottom electrode, a piezoelectric layer, and a top electrode, wherein the piezoelectric layer is positioned between the bottom electrode and the top electrode.
    Type: Grant
    Filed: December 4, 2017
    Date of Patent: October 22, 2019
    Assignee: QORVO US, INC.
    Inventors: James Russell Webster, Peter J. Schiller, Richard Allan Van Deusen, Ian Robert Harmon
  • Patent number: 10391496
    Abstract: Methods, devices, systems, and kits useful for the collection and analysis of samples obtained by swabs are disclosed. Swab containers configured for receiving a swab containing a sample; cartridges for holding one or more of: a swab container, a swab, assay units, pipette tips, vessels, transport units, and implements; systems (which may include a sample processing device); kits; and methods for their use are disclosed. A swab container may include an entry port; an assay chamber having an assay port; a conduit comprising an interior channel connecting the entry port; and an interior channel providing fluidic communication between the entry port and assay chamber. An interior channel may be configured to squeeze a portion of a swab placed in or through the conduit. A cartridge may include a cartridge frame configured to receive one or more of swab containers, assay units, transport units, pipette tips, vessels or implements.
    Type: Grant
    Filed: February 11, 2016
    Date of Patent: August 27, 2019
    Assignee: Theranos IP Company, LLC
    Inventors: Clarissa Lui, Elizabeth A. Holmes
  • Patent number: 10300480
    Abstract: A pipetting device (100) for an apparatus (164) for processing a sample or reagent is disclosed. The pipetting device (100) comprises a coupling mechanism (102), wherein the coupling mechanism (102) comprises at least a first coupling unit (104) adapted to be coupled to a first pipetting tip and a second coupling unit (106) adapted to be coupled to a second pipetting tip, and a tilt mechanism (112) for moving the first coupling unit (104) between an untilted position, in which the first coupling unit (104) and the second coupling unit (106) are arranged parallel to one another, and a tilted position, in which the first coupling unit (104) is tilted relative to the second coupling unit (106).
    Type: Grant
    Filed: October 12, 2016
    Date of Patent: May 28, 2019
    Assignee: Roche Molecular Systems, Inc.
    Inventors: Hans-Rudolf Bachmann, Rolf Schneebeli
  • Patent number: 10265698
    Abstract: Microfluidic structures featuring substantially circular channels may be fabricated by embossing polymer sheets.
    Type: Grant
    Filed: November 6, 2015
    Date of Patent: April 23, 2019
    Assignees: The Charles Stark Draper Laboratory, Inc., The Brigham and Women's Hospital, Inc., The Massachusetts Institute of Technology
    Inventors: Jeffrey T. Borenstein, Eli J. Weinberg, James C. Hsiao, Ahmad S. Khalil, Malinda M. Tupper, Guillermo Garcia-Cardena, Peter Mack, Sarah L. Tao
  • Patent number: 10260063
    Abstract: Provided herein is technology related to processing samples of nucleic acids and particularly, but not exclusively, to methods for enriching samples for small nucleic acids, such as small circulating cell-free DNA.
    Type: Grant
    Filed: October 9, 2017
    Date of Patent: April 16, 2019
    Assignee: ABBOTT MOLECULAR INC.
    Inventors: Dae Hyun H. Kim, Gerard J. Gundling, Herbert A. Marble, Mark W. Eshoo
  • Patent number: 10258981
    Abstract: A measurement device containing one or more capillaries to measure the volume of a dispensed fluid and determine the volumetric accuracy of the dispensing device. The measurement device can contain a reservoir containing the fluid to be measured and there may be an additional reservoir for a secondary fluid. A viewing window is necessary to complete a manual measurement and may include a magnifying lens. The liquid well by the capillary inlet may be shaped such that the measurement fluid is directed toward the capillary. The well may have features designed to position the dispensing device toward the capillary, or to position the well proximal to the capillary after it is filled. The well may also have surfaces or coatings which attract or do not attract various types of substances. The measurement device may interface with sensors to output measurement data.
    Type: Grant
    Filed: December 12, 2016
    Date of Patent: April 16, 2019
    Assignee: Forward Biotech, Inc.
    Inventors: Ian K. Glasgow, Brian Kolb, Andrea Habura
  • Patent number: 10228363
    Abstract: Disclosed is a sample dispensing method. The method comprises: suctioning a urine sample from a sample container by a first nozzle; moving the first nozzle to a holding chamber after the first nozzle has suctioned the urine sample from the sample container, and discharging the urine sample into the holding chamber; moving the first nozzle to a first wash tank and washing the first nozzle in the first wash tank after the first nozzle has discharged the urine sample into the holding chamber; and moving a second nozzle to the holding chamber after the first nozzle has started moving to the first wash tank, and moving the second nozzle which has suctioned the urine sample from the holding chamber to respective plurality of processing chambers and discharging a part of the urine sample to the respective plurality of processing chambers.
    Type: Grant
    Filed: March 28, 2016
    Date of Patent: March 12, 2019
    Assignee: SYSMEX CORPORATION
    Inventors: Yuta Okamura, Ryuichiro Ebi, Masahiko Oguro, Go Senda
  • Patent number: 10222333
    Abstract: The present disclosure is directed to a method of detecting a molecule attached to a surface comprising detecting a bead attached to the molecule. The method may be used for the detection of single molecules and the surface shape may be adapted to receive beads. The disclosure further describes an apparatus for detecting the presence of a molecule having a bead attached thereto comprising a surface adapted to detect a bead wherein the shape of the surface is configured to receive and retain a bead. Kits comprising the apparatus are provided and a use of the apparatus for detecting a bead or a molecule attached thereto is also described.
    Type: Grant
    Filed: February 24, 2010
    Date of Patent: March 5, 2019
    Assignee: GENESEQUE AS
    Inventor: Jarle Kotsbak
  • Patent number: 10180419
    Abstract: An apparatus for measuring blood clotting time includes a blood clot detection instrument and a cuvette for use with the blood clot detection instrument. The cuvette includes a blood sample receptor-inlet; a channel arrangement including at least one test channel for performing a blood clotting time measurement, a sampling channel having at least one surface portion that is hydrophilic, communicating with the blood sample receptor-inlet and the at least one test channel, and a waste channel having at least one surface portion that is hydrophilic, communicating with the sampling channel; and a vent opening communicating with the sampling channel. The sampling channel, the vent opening and the waste channel, coact to automatically draw a requisite volume of a blood sample deposited at the blood receptor-inlet, into the sampling channel.
    Type: Grant
    Filed: August 5, 2013
    Date of Patent: January 15, 2019
    Assignee: Instrumentation Laboratory Company
    Inventors: Gregory M. Colella, Henry D. Huang, Anthony F. Kuklo, Jr., Dimitri Shishkin, Maria Figueroa, James A. Mawhirt
  • Patent number: 10174277
    Abstract: A media tray system may include a tray having a base wall and a perimeter wall extending upwardly from the base wall and having an upper edge. The system may also include a cover having a top wall and a side wall extending downwardly from the top wall to extend about the perimeter wall of the tray when the cover is in a closed condition with respect to the tray, with the side wall having a lower edge. The system may further include a cover support structure located at the first end of the tray and the first end of the first end of the cover. The cover support structure may be configured to support the cover in an access condition permitting access to the tray interior of the tray through a gap formed between an upper edge of the tray and the lower edge of the cover.
    Type: Grant
    Filed: January 4, 2017
    Date of Patent: January 8, 2019
    Inventor: John L. Sternick
  • Patent number: 10168259
    Abstract: One aspect of the present disclosure can include a microfluidic device for imaging a tissue sample. The device can include a tissue chamber, a liquid inlet channel, and a liquid outlet channel. The tissue chamber can be defined by a plurality of walls, at least one of which is transparent. The liquid inlet channel can be fluid communication with the tissue chamber. The liquid outlet channel can be in fluid communication with the tissue chamber. The tissue chamber can be sized and dimensioned to completely immobilize the tissue sample during imaging.
    Type: Grant
    Filed: June 12, 2015
    Date of Patent: January 1, 2019
    Assignee: University of Notre Dame
    Inventors: Siyuan Zhang, Jeremiah Zartman, David Hoelzle, Wendy Alvarez Barrios, Victoria Zellmer, Cody Narciso
  • Patent number: 10168341
    Abstract: A device may be configured to allow individual measuring of at least one property of at least one cell, such as measuring a contraction force of a platelet. The device may include a plurality of wells. Each well may include a hydrogel layer, the hydrogel layer including a hydrogel having a top surface that includes a pattern of cell interaction regions. The wells may differ in stiffness properties of the hydrogel and/or biochemical conditions. Each cell interaction region may include a group of at least two cell interaction sites. The spacing between each cell interaction region may be greater than a spacing between the at least two cell interaction sites of each cell interaction region. In this way, cell-cell interactions may be reduced and thereby increasing number of individual cells capable of being measured.
    Type: Grant
    Filed: March 10, 2014
    Date of Patent: January 1, 2019
    Assignees: Emory University, Children's Healthcare of Atlanta, Inc.
    Inventors: Wilbur A. Lam, David Myers, Yongzhi Qiu
  • Patent number: 10137448
    Abstract: A microfluidic chip is disclosed herein. In an embodiment, the microfluidic chip includes a body including at least one microfluidic pathway configured to receive a fluid sample, the at least one microfluidic pathway including a coating configured to reduce fluid diffusion and seal a surface of the at least one microfluidic pathway, and a heating device located on the body and forming a heating zone within a portion of the at least one microfluidic pathway.
    Type: Grant
    Filed: April 20, 2017
    Date of Patent: November 27, 2018
    Assignee: FluxErgy, LLC
    Inventors: Tej Rushikesh Patel, Ryan Alan Revilla, Matthew D'Ooge
  • Patent number: 10131938
    Abstract: The present invention is directed to a method of designing a plurality of capture oligonucleotide probes for use on a support to which complementary oligonucleotide probes will hybridize with little mismatch, where the plural capture oligonucleotide probes have melting temperatures within a narrow range. The present invention further relates to an oligonucleotide array comprising of a support with the plurality of oligonucleotide probes immobilized on the support, a method of using the support to detect single-base changes, insertions, deletions, or translocations in a plurality of target nucleotide sequences, and a kit for such detection, which includes the support on which the oligonucleotides have been immobilized.
    Type: Grant
    Filed: July 7, 2017
    Date of Patent: November 20, 2018
    Assignee: Cornell Research Foundation, Inc.
    Inventors: Francis Barany, Monib Zirvi, Norman P. Gerry, Reyna Favis, Richard Kliman
  • Patent number: 10130283
    Abstract: The invention relates to an ingestible, implantable or wearable medical device comprising a microarray which comprises a bioactive agent capable of interacting with a disease marker biological analyte; a reservoir which comprises at least one therapeutic agent and is capable of releasing the therapeutic agent(s) from the medical device; and a plurality of microchips comprising a microarray scanning device capable of obtaining physical parameter data of an interaction between the disease marker biological analyte with the bioactive agent; a biometric recognition device capable of comparing the physical parameter data with an analyte interaction profile; optionally a therapeutic agent releasing device capable of controlling release of the therapeutic agent from the reservoirs; an interface device capable of facilitating communications between the microarray scanning device, biometric recognition device and the therapeutic agent releasing device; and an energy source to power the medical device.
    Type: Grant
    Filed: August 3, 2015
    Date of Patent: November 20, 2018
    Assignee: Theranos, IP Company, LLC
    Inventors: Elizabeth A. Holmes, Shaunak Roy, John Howard, Chengwang Wang
  • Patent number: 10101307
    Abstract: Systems, apparatus, methods, and kits are provided for automated mass spectrometric analysis of small volumes of liquid samples, such as biological samples. The systems, apparatus, and kits may be used in facilities where high throughput of samples, as well as reliable and repeatable assay results with little training of staff, are needed. Such facilities include hospital emergency wards.
    Type: Grant
    Filed: December 28, 2016
    Date of Patent: October 16, 2018
    Assignee: PureHoney Technologies, Inc.
    Inventor: Can Ozbal
  • Patent number: 10101272
    Abstract: A sample detection plate according to the present disclosure includes a first substrate having a first surface, and a sample container that is provided on the first surface of the first substrate to contain a sample that absorbs electromagnetic waves having a predetermined wavelength. The first substrate includes a first material that emits autofluorescence in response to the electromagnetic waves having the predetermined wavelength.
    Type: Grant
    Filed: August 2, 2016
    Date of Patent: October 16, 2018
    Assignee: PANASONIC INTELLECTUAL PROPERTY MANAGEMENT CO., LTD.
    Inventors: Kenji Nagatomi, Masaya Nakatani
  • Patent number: 10099220
    Abstract: The invention relates to a method for isolating at least one fluid sample well laid out in a test card for analyses, said test card including a plate (3) having at least one first main face from which is laid out at least one channel communicating with at least said well, this first face being coated with a membrane (17) provided with an adhesive (18) and which will cover said channel with a covering area (171), the method for isolation including a phase for introducing a fluid sample into at least said well and a phase for isolating said well consisting of exerting a force on at least one portion of the covering area (171) of the adhesive membrane (17) for ensuring displacement of the adhesive into an obturation area for the sample well.
    Type: Grant
    Filed: April 28, 2016
    Date of Patent: October 16, 2018
    Assignee: BIOMERIEUX
    Inventors: Bruno Colin, Cecile Paris
  • Patent number: 10077420
    Abstract: The invention generally relates to containers for cell and tissue culturing with multiple compartments in fluid communication with each other to provide a common culture environment in each of the compartments while maintaining physical separation of cells and tissue therein. The invention further relates to culture containers providing a sterile culture environment with detachably coupleable lids and open access to each compartment within a container.
    Type: Grant
    Filed: December 2, 2014
    Date of Patent: September 18, 2018
    Assignee: Histogenics Corporation
    Inventor: Eric Blahut
  • Patent number: 10046322
    Abstract: This disclosure relates to an apparatus for simultaneously filling a plurality of sample chambers. In one aspect, the apparatus comprises a common fluid source and a plurality of independent, continuous fluidic pathways. Each independent, continuous fluidic pathway comprises a sample chamber and a pneumatic compartment. The sample chamber is connected to the common fluid source, and the pneumatic compartment is connected to the sample chamber. The sample chamber comprises, in part, an assay chamber. The assay chamber comprises a monolithic substrate and a plug. In some embodiments, the assay chamber contains a magnetic mixing element. In some embodiments, the assay chamber is a double tapered chamber. In some embodiments, a ratio of a volume of the sample chamber to a volume of the pneumatic compartment is substantially equivalent for each fluidic pathway of the plurality of fluidic pathways.
    Type: Grant
    Filed: March 22, 2018
    Date of Patent: August 14, 2018
    Assignee: TALIS BIOMEDICAL CORPORATION
    Inventor: Thomas H. Cauley, III
  • Patent number: 10039795
    Abstract: Composition and methods for the detection of one or more target microbe(s) are provided. Compositions of the disclosure include at least one recombinant phage capable of infecting a target microbe, said phage comprising at least a capsid protein sequence, a ribosome binding site, and a codon-optimized marker. Compositions of the disclosure may further include an aqueous solution that enhances the ability to detect marker expression upon phage infection of the target microbe. In some embodiments the target microbe include is Listeria.
    Type: Grant
    Filed: May 8, 2015
    Date of Patent: August 7, 2018
    Assignee: Institute for Environmental Health, Inc.
    Inventors: Michael Sandor Koeris, Jayson L. Bowers, Daniel Robert Brownell, Edyta Krzymanska-Olejnik, Robert Patrick Shivers, Michael Cappillino
  • Patent number: 10024863
    Abstract: According to the present teachings, compositions, kits, and methods for protein melt analysis are provided that utilizing one or more fluorophore dyes. In some embodiments, a method comprises preparing a sample by mixing at least one protein with two or more dyes, and applying a controlled heating, while recording the fluorescence emission of the sample. The methods can be used, for example, for screening conditions for optimized protein stability, screening for ligands that bind and enhance protein stability (e.g., protein-protein interactions), screening for mutations for enhanced stability, screening crystallization conditions for protein stability, screening storage conditions for protein stability, and screening conditions in which a protein will be used (e.g., production conditions, treatment conditions, etc.) for protein stability.
    Type: Grant
    Filed: March 14, 2014
    Date of Patent: July 17, 2018
    Assignee: LIFE TECHNOLOGIES CORPORATION
    Inventors: Carole Bornarth, Mousumi Rath
  • Patent number: 9987630
    Abstract: A fluid handling device includes: a first channel through which liquid flows by capillarity; a liquid reservoir which communicates with an upstream end of the first channel and stores liquid; a liquid introduction part which communicates with the liquid reservoir and includes a taper part whose diameter decreases from an opening part toward the liquid reservoir; a stop valve disposed on a downstream end of the first channel and including a step part where a cross-sectional area of the channel in a direction orthogonal to a direction in which liquid flows discontinuously increases; and a second channel which communicates with a downstream end of the first channel, the second channel being a channel through which fluid flows.
    Type: Grant
    Filed: July 6, 2015
    Date of Patent: June 5, 2018
    Assignee: ENPLAS CORPORATION
    Inventor: Hayato Inoue
  • Patent number: 9988676
    Abstract: A microfluidic cartridge can include at least one nucleic acid analysis portion. Each nucleic acid analysis portion can include a fluidic network being configured for micro-liter volumes or less, a sample input at the beginning of the fluidic network, a plurality of vent ports and fluidic channels in the fluidic network configured to effectuate hydrodynamic movement within the fluidic network, an extraction mixture reservoir in the fluidic network, a mixing chamber in the fluidic network, an amplification chamber in the fluidic network, and a separation channel in the fluidic network. A nucleic acid analyzer can be capable of performing nucleic acid analysis using the microfluidic cartridge. A nucleic acid analysis method can be performed using the microfluidic cartridge.
    Type: Grant
    Filed: June 19, 2015
    Date of Patent: June 5, 2018
    Assignee: LEIDOS INNOVATIONS TECHNOLOGY, INC.
    Inventors: Michael E. Egan, Brian Root, Orion N. Scott, Douglas J. South, Joan M. Bienvenue, Paul Kinnon, James Landers, David Saul, An-Chi Tsuei, Jason Hayes, Matthew Springer, Matthew Solomon, Peter Van Ruijven
  • Patent number: 9975117
    Abstract: Provided is an apparatus and method for controlling a droplet. The apparatus for controlling the droplet does not contaminate/damage a sample, has a high degree of freedom in droplet control, and is capable of being repeatedly used for a long time. An apparatus (100) for controlling a droplet according to an embodiment includes a flexible substrate (120) having a hydrophobic or oleophobic surface, a dimple formation unit (140), which locally deforms a bottom surface of the flexible substrate (120) to form a dimple, and a driving unit (160), which moves the dimple formation unit (140) at a lower side of the flexible substrate.
    Type: Grant
    Filed: May 5, 2016
    Date of Patent: May 22, 2018
    Assignee: Industry-Academic Cooperation Foundation, Yonsei University
    Inventors: Taeyoon Lee, Jonghyun Ahn, Jungmok Seo, Seoung Ki Lee
  • Patent number: 9977401
    Abstract: The invention provides a method, apparatus and system for separating blood and other types of cellular components, and can be combined with holographic optical trapping manipulation or other forms of optical tweezing. One of the exemplary methods includes providing a first flow having a plurality of blood components; providing a second flow; contacting the first flow with the second flow to provide a first separation region; and differentially sedimenting a first blood cellular component of the plurality of blood components into the second flow while concurrently maintaining a second blood cellular component of the plurality of blood components in the first flow. The second flow having the first blood cellular component is then differentially removed from the first flow having the second blood cellular component. Holographic optical traps may also be utilized in conjunction with the various flows to move selected components from one flow to another, as part of or in addition to a separation stage.
    Type: Grant
    Filed: April 15, 2016
    Date of Patent: May 22, 2018
    Assignee: PREMIUM GENETICS (UK) LTD.
    Inventors: Daniel Mueth, Joseph Plewa, Jessica Shireman, Amy Anderson, Lewis Gruber, Neil Rosenbaum
  • Patent number: 9976954
    Abstract: A microfluidic device includes a chamber configured to accommodate a sample and air, and a detection device provided with a light emitter configured to emit light towards the chamber on a light transmission path and a light receiver configured to receive the light, and further configured to measure an optical density of the chamber to determine whether the sample is accommodated in the chamber based on the received light. A boundary between the sample and the air accommodated in the chamber is provided on the light transmission path.
    Type: Grant
    Filed: September 16, 2015
    Date of Patent: May 22, 2018
    Assignee: SAMSUNG ELECTRONICS CO., LTD.
    Inventors: Yeong Bae Yeo, Jung Ki Min
  • Patent number: 9971136
    Abstract: The invention relates to a method and a system to achieve spatially (e.g. three-dimensionally) confined photomodulation at the focal volume (50) in a ample (55) mounted in a microscope system, comprising two or more laser light sources (41, 42) emitting light (32, 34) of different wavelengths adapted to excite a material in an identical number of independent excitation steps to a higher vibrational state from which the material relaxes, either emitting a conversion light to be detected (“photoexcitation”) or modulating the spectral properties of the material (“photomodulation”).
    Type: Grant
    Filed: March 20, 2014
    Date of Patent: May 15, 2018
    Assignees: ETH ZÜRICH, CALIFORNIA INSTITUTE OF TECHNOLOGY
    Inventors: Periklis Pantazis, William P. Dempsey, Thai V. Truong, Scott E. Fraser, Lada Georgieva
  • Patent number: 9943844
    Abstract: This applicator comprises a base (22) and a product applicator tongue (25) that projects from the base (22) along an axis (A-A?), said tongue (25) defining a central cavity (30). It comprises a massage element (32) disposed in the central cavity (30), with the massage (32) element and the tongue (25) defining therebetween in the cavity (30) an intermediary product retaining space (33). The massage element (32) and the tongue (25) can move in relation to one another transversally in relation to the axis (A-A?).
    Type: Grant
    Filed: April 9, 2014
    Date of Patent: April 17, 2018
    Assignee: L'OREAL
    Inventors: Fréderic Baltenneck, Jean-Baptiste Galey, Michel Dailly, Anne Colonna, Fabrice Monti