Liquid Moving Means Patents (Class 422/504)
  • Patent number: 11453002
    Abstract: The present disclosure provides a microfluidic device configured to deliver a controlled amount of a liquid to an analysis tool, wherein the microfluidic device comprises: a buffer tank configured to contain a liquid and/or a gas; at leas one level sensor configured to measure a liquid level in the buffer tank; a pneumatic system configured to create selectively a positive or a negative pressure in the buffer tank; at least one intake port to let in a liquid in the buffer tank; at least one delivery port to inject a controlled amount of liquid from the buffer tank onto the analysis tool; at least one drain port with a controlled drain valve, located on the lower part of the buffer tank to discharge the liquid from the buffer tank; a first check valve located upstream of the intake port and a second check valve between the buffer tank and the analysis tool.
    Type: Grant
    Filed: December 14, 2020
    Date of Patent: September 27, 2022
    Assignee: WITHINGS
    Inventors: Ianis Oueslati, Charlotte Leger, Marine Kirat
  • Patent number: 11383240
    Abstract: The present invention relates to, inter alia, a microfluidic device for performing single cell genomic DNA isolation and amplification under flow. The microfluidic device comprises a solid substrate having one or more microfluidic channel system formed therein. Each microfluidic channel system of the microfluidic device comprises: (a) an intake region comprising a single microchannel; (b) a plurality of cell segregation microchannels; (c) a cell capture site located downstream of each cell segregation microchannel; and (d) a DNA capture array positioned downstream of the cell capture site and comprising a plurality of micropillars. Also disclosed is a whole genome amplification system that includes the microfluidic device of the present disclosure, as well as a method for conducting single cell DNA analysis via on-chip whole genome amplification while under flow, and a method for multiple displacement amplification (MDA) reactions of one or more nucleic acid sequence isolated single cells.
    Type: Grant
    Filed: May 22, 2017
    Date of Patent: July 12, 2022
    Assignee: CORNELL UNIVERSITY
    Inventors: Harold G. Craighead, Harvey C. Tian
  • Patent number: 11383230
    Abstract: A digital dispense apparatus includes at least one fluid dispense device, at least one reservoir fluidically connected to the at least one fluid dispense device, a monolithic carrier structure carrying the at least one fluid dispense device and reservoir, the monolithic carrier forming fluid routing between the reservoir and the fluid dispense device.
    Type: Grant
    Filed: March 31, 2016
    Date of Patent: July 12, 2022
    Assignee: Hewlett-Packard Development Company, L.P.
    Inventors: Jeffrey A. Nielsen, Michael W. Cumbie, Devin Alexander Mourey, Silam J. Choy, Kenneth Ward, Christie Dudenhoefer
  • Patent number: 11385251
    Abstract: A pretreatment apparatus includes a pretreatment container placement section where a pretreatment container which is housing a specimen holding member including a microchannel for holding a specimen is placed; a carrying mechanism for carrying the pretreatment container that is placed at the pretreatment container placement section; and a pretreatment section including a port where the pretreatment container that is carried by the carrying mechanism is placed, the pretreatment section being configured to perform pretreatment including a shaking process of shaking the pretreatment container to extract the specimen from the specimen holding member in the pretreatment container that is placed in the port.
    Type: Grant
    Filed: January 31, 2019
    Date of Patent: July 12, 2022
    Assignee: SHIMADZU CORPORATION
    Inventor: Nobuhiro Hanafusa
  • Patent number: 11369961
    Abstract: The present invention provides an apparatus and method for washing and concentrating microparticles encapsulated in microscale droplets using an acoustic radiation force. The apparatus includes: a piezoelectric substrate; a slanted finger interdigital transducer (SIDT) electrode deposited on the piezoelectric substrate and configured to generate surface acoustic waves under an AC signal applied thereto; and a microfluidic chip which is adhered to the piezoelectric substrate with being spaced apart from the SIDT electrode, has a microscale channel section formed therein, in which a single continuous phase and a plurality of dispersed phases are injected, respectively, and includes a plurality of inlet ports into which continuous and dispersed phases are injected, and a discharge port from which a plurality of droplets composed of the continuous and dispersed phases and generated by the intersection thereof are discharged.
    Type: Grant
    Filed: December 6, 2019
    Date of Patent: June 28, 2022
    Assignee: KOREA ADVANCED INSTITUTE OF SCIENCE AND TECHNOLOGY
    Inventors: Hyung-Jin Sung, Jinsoo Park, Ghulam Destgeer, Yeunwoo Cho, Hyoungsoo Kim
  • Patent number: 11338293
    Abstract: A microfluidic device includes a first substrate and a second substrate opposite to each other. A light-emitting layer, a first driving layer, and a first hydrophobic layer are on surface of the first substrate facing the second substrate; and first hydrophobic layer is disposed near second substrate; a photosensitive layer, a second driving layer and a second hydrophobic layer are on surface of the second substrate facing the first substrate; and second hydrophobic layer is disposed near first hydrophobic layer, and a gap for holding a droplet is between second hydrophobic layer and first hydrophobic layer; the first and second driving layers are configured to drive the droplet to move within the gap when applied with a driving voltage; the light-emitting layer is configured to emit light with a set wavelength toward the gap; and the photosensitive layer is configured to generate an induced current according to received light.
    Type: Grant
    Filed: November 19, 2019
    Date of Patent: May 24, 2022
    Assignees: BEIJING BOE DISPLAY TECHNOLOGY CO., LTD., BOE TECHNOLOGY GROUP CO., LTD.
    Inventors: Jinyu Ren, Guojing Ma
  • Patent number: 11293916
    Abstract: A method comprises adding reagent I to a sample to be detected, incubating to obtain a first detection sample, and detecting to obtain a first absorbance value A1 of the sample to be detected; adding reagent II to the first detection sample, incubating to obtain a second detection sample, and detecting to obtain a second absorbance value A2 of the sample to be detected; adding reagent I to a standard sample, incubating to obtain a first detection standard, and detecting to obtain a first absorbance value B1 of the standard; adding reagent II to the first detection standard, incubating to obtain a second detection standard, and detecting to obtain a second absorbance value B2 of the standard; and using A1 and A2, and B1 and B2 to obtain a concentration level of complement factor H in the sample to be detected.
    Type: Grant
    Filed: December 9, 2016
    Date of Patent: April 5, 2022
    Assignees: PEKING UNIVERSITY FIRST HOSPITAL
    Inventors: Zhongling Liu, Minghui Zhao, Feng Yu
  • Patent number: 11285476
    Abstract: A microfluidic device includes a substrate having a first fluid inlet/outlet system, a second fluid inlet/outlet system, and a fluidic network between the first fluid inlet/outlet system and the second fluid inlet/outlet system and in fluid communication with the first fluid inlet/outlet system and the second fluid inlet/outlet system. The fluidic network includes a microfluidic channel network that is in fluid communication with the first fluid inlet/outlet system and spaced from the second fluid inlet/outlet system, a nanofluidic channel network fluidly connecting the microfluidic channel network and the second fluid inlet/outlet system, and a plurality of pores in fluid communication with the microfluidic channel network and the nanofluidic channel network.
    Type: Grant
    Filed: August 29, 2019
    Date of Patent: March 29, 2022
    Assignee: INTERFACE FLUIDICS LTD.
    Inventors: Ali Abedini, Adam Pettigrew, Bo Bao, Thomas De Haas, Stuart Kinnear, Scott Pierobon
  • Patent number: 11278896
    Abstract: Provided is a microfluidic device to more easily and effectively operate a valve by improving the structure of valves for controlling a fluid flow more simply and efficiently, which comprises a platform having at least one chamber, at least one flow channel connected to the chambers and transfer fluid, and a valve which opens or closes the flow channel, wherein the valve comprises a body installed in the platform, a blocking plate installed in the body and positioned to face the flow channel to selectively blocks the flow channel, a pressing rod installed to be movable at the inside of the body to press the blocking plate, and a fixing unit installed at the body and fix the pressing rod at a blocking plate pressing position.
    Type: Grant
    Filed: December 22, 2017
    Date of Patent: March 22, 2022
    Assignee: UNIST(ULSAN NATIONAL INSTITUTE OF SCIENCE AND TECHNOLOGY)
    Inventors: Yoon Kyoung Cho, Kyusang Lee, Yongjin Lee
  • Patent number: 11280681
    Abstract: The present disclosure relates to a flexible temperature sensor, a method for preparing the same, and a flexible device. The flexible temperature sensor includes: a flexible substrate; an electrode arranged on the flexible substrate; a mixed fluid arranged on the flexible substrate and in contact with the electrode, in which the mixed fluid includes an ionic liquid and porous conductive particles; and a protective plate arranged on a surface of the flexible substrate having the mixed fluid to protect the mixed fluid.
    Type: Grant
    Filed: September 6, 2019
    Date of Patent: March 22, 2022
    Assignees: BEIJING BOE DISPLAY TECHNOLOGY CO., LTD., BOE TECHNOLOGY GROUP CO., LTD.
    Inventors: Jinchao Bai, Xun Wang, Zumou Wu, Xiaolong Li, Tianyu Zuo, Xiangqian Ding
  • Patent number: 11266988
    Abstract: The invention, is directed to devices and methods for low cost and convenient separation, of plasma from whole blood. In some embodiments, devices of the invention comprise an integrated collection of channels and chambers m a body that permit acquisition of a blood sample by capillary action, centrifugal separation of cells from plasma, and manual dispensing of purified plasma by simple pinching of a bellows chamber to force air into plasma-holding channels which thereby expels a predetermined volume of the purified plasma.
    Type: Grant
    Filed: March 14, 2018
    Date of Patent: March 8, 2022
    Inventors: Hrair Kirakossian, Ming Tan
  • Patent number: 11209394
    Abstract: A cartridge for sample handling and sensing includes (i) a sample port; (ii) a first fluid port connected to the sample reservoir in the distal region via a first fluid channel; and (iii) a second fluid port connected to the sample reservoir via a second fluid channel. The cartridge includes (i) a sensor platform comprising a bulk acoustic wave (BAW) resonator and a fluid flow path comprising a sensing region extending across a sensing surface of the BAW resonator; and (ii) a fluid valve between the sample reservoir and the sensing region. A sample may be applied to the sample port; first volume of fluid may be injected through the first fluid port; and then a second volume of fluid may be injected through the second fluid port to drive the sample into the sensing region of the fluid flow path.
    Type: Grant
    Filed: July 26, 2017
    Date of Patent: December 28, 2021
    Assignee: Qorvo US, Inc.
    Inventors: Thayne L. Edwards, Florian Bell, Matthew Ryder, Bruce Murdock
  • Patent number: 11148134
    Abstract: An assay device for use with samples of biological fluid, the assay device comprising: a physical detection unit for measuring a viscosity of a biological fluid sample; and a biomarker detection unit for detecting and/or measuring a biomarker in the biological fluid sample, wherein the physical detection unit and the biomarker detection unit act together as part of an assay process.
    Type: Grant
    Filed: February 23, 2017
    Date of Patent: October 19, 2021
    Assignee: SEPSENSE LIMITED
    Inventors: Peter Hull, Jimish Patel
  • Patent number: 11141708
    Abstract: A cell culture apparatus, includes a connected culture container including n number of units disposed in parallel along a second direction that is a different direction from a first direction where each of the n number of the units is constituted of m number of culture chambers and one or more communication-channels, the m number of the culture chambers each having a cell-holding portion that holds seeded cells, the m number of the culture chambers storing liquid culture media, the m number of the culture chambers being disposed in parallel along the first direction, the communication-channels communicating the m number of the culture chambers with each other; and a plurality of pneumatic pipes communicating same-row-disposed culture chambers that are disposed on a same row along the second direction with each other in the connected culture container.
    Type: Grant
    Filed: March 8, 2016
    Date of Patent: October 12, 2021
    Inventors: Shinji Sugiura, Taku Satoh, Toshiyuki Kanamori, Kazumi Shin
  • Patent number: 11142738
    Abstract: Methods and systems for cell media exchange wherein media maybe aspirated without also aspirating cells and wherein removal and re-plating of cells is not necessarily required. For example, the cell culture system or apparatus features a gap that is small enough to retain cells therein and also sized to prevent media from leaking. The methods and systems of the present invention may help reduce stress and damage to cells as compared to traditional methods that require removing and re-plating cells.
    Type: Grant
    Filed: September 28, 2017
    Date of Patent: October 12, 2021
    Assignee: THE REGENTS OF THE UNIVERSITY OF CALIFORNIA
    Inventor: Lawrence Kulinsky
  • Patent number: 11119084
    Abstract: The invention provides a method and device for measuring mercury isotopes in natural gas. The method includes the following steps: (1) primary enrichment: subjecting natural gas to a three-stage cascading absorption with an acidic potassium permanganate aqueous solution, and collecting all of the acidic potassium permanganate aqueous solutions in which natural gas is absorbed in step (1); (2) mercury purification and enrichment: reducing the mercury absorbed in the step (1) to mercury vapor with a stannous chloride solution, and then purifying and enriching the mercury vapor by using an acidic potassium permanganate aqueous solution; (3) detecting the acidic potassium permanganate solution in which the mercury vapor is enriched in step (2) to determine the total mercury content therein; and (4) detecting the acidic potassium permanganate solution in which the mercury vapor is enriched in step (2) to determine the composition/content of stable mercury isotopes therein.
    Type: Grant
    Filed: April 19, 2019
    Date of Patent: September 14, 2021
    Assignee: PetroChina Company Limited
    Inventors: Guangyou Zhu, Shunlin Tang
  • Patent number: 11119085
    Abstract: A method and device for detecting mercury isotopes in an oil-gas source. The device includes at least one enrichment-absorption system for mercury in crude oil/hydrocarbon source rock, an enrichment-absorption system for mercury in natural gas and at least one secondary purification-enrichment system for mercury. The enrichment-absorption system for mercury in crude oil/hydrocarbon source rock includes three air-absorption bottles, a pyrolysis/cracking system, five impact samplers, and a vacuum pump, which are connected in series by pipe lines. The enrichment-absorption system for mercury in natural gas includes five impact samplers connected in series, wherein the first impact sampler is connected to the natural gas outlet from the natural gas well and the last impact sampler is connected to the cumulative gas flow meter.
    Type: Grant
    Filed: April 24, 2019
    Date of Patent: September 14, 2021
    Assignee: PetroChina Company Limited
    Inventors: Guangyou Zhu, Shunlin Tang
  • Patent number: 11103872
    Abstract: A positioning system for positioning a consumable rack in a diagnostic system is disclosed. The positioning system comprises a rack comprising an upper surface comprising holding positions and a receiving compartment comprising a rectangular chassis comprising front, rear, and two lateral sides. The receiving compartment comprises a holding structure coupled to the chassis to move between first and second positions. The rack comprises sidewalls. At least three sidewalls have a center alignment element. The chassis comprises three chassis alignment elements on the rear and two lateral sides. The holding structure comprises a corner push element between the chassis front and lateral sides to push against a side edge of the rack between two sidewalls when the holding structure is moved from the first position towards the second position forcing the three alignment elements against a chassis alignment element and laterally holding the rack in position by the chassis alignment elements.
    Type: Grant
    Filed: August 31, 2018
    Date of Patent: August 31, 2021
    Assignee: Roche Diagnostics Operations, Inc.
    Inventors: Renato Belz, Armin Bucher, Christian Thalmann, Reto Joho, Thomas Huber
  • Patent number: 11085921
    Abstract: The present invention relates to a sensor for an electronic tongue or nose for analysing a sample or detecting a target. The sensor comprises a support, on one surface of which a plurality of sensitive areas are located, each sensitive area comprising at least one receptor and being capable of transmitting a measurable signal generated by the interaction of at least one constituent of the sample or one target with at least one receptor. The sensor is characterised in that it comprises at least three sensitive areas that differ from one another in terms of their respective receptor compositions, at least one of the sensitive areas comprising a mixture of at least two different receptors, while the two other sensitive areas each comprise at least one of the two receptors.
    Type: Grant
    Filed: February 21, 2013
    Date of Patent: August 10, 2021
    Assignees: Commissariat a l'Energie Atomique et aux Energies Alternatives, Centre National de la Recherche Scientifique, Universite Paris-Sud
    Inventors: Thierry Livache, Arnaud Buhot, David Bonnaffe, Yanxia Hou-Broutin
  • Patent number: 11073515
    Abstract: The invention is directed to a method for detecting a target moiety in a sample of biological specimens by: a) providing at least one conjugate with the general formula (I) Xn—P—Ym, b) contacting the sample of biological specimens with at least one conjugate, thereby labeling the target moiety recognized by the antigen recognizing moiety Y with the conjugate c) exciting the labelled target moieties with light having a wavelength within the absorbance spectrum of fluorescent moiety X d) detecting the labelled target moieties by detecting the fluorescence radiation and e) degrading the fluorescent moiety X of the labelled target moieties by irradiating the conjugate with light having a wavelength within the absorbance spectrum of fluorescent moiety X Use of the method in fluorescence microscopy, flow cytometer, spectrofluorometry, cell separation, pathology or histology.
    Type: Grant
    Filed: November 16, 2018
    Date of Patent: July 27, 2021
    Assignee: Miltenyi Biotec GmbH
    Inventors: Jonathan Fauerbach, Christian Dose, Thomas Rockel, Veronika Rudolf
  • Patent number: 11067570
    Abstract: The present disclosure relates to a bio-detection chip and a detection method associated therewith. The bio-detection chip includes an upper substrate, a lower substrate, a reference electrode, a driving electrode, and a first dielectric layer, a first hydrophobic layer, a second hydrophobic layer and a second dielectric layer disposed successively between the reference electrode and the driving electrode. The bio-detection chip further includes a plurality of micro-capsules arranged between the first hydrophobic layer and the second hydrophobic layer. Each micro-capsule encapsulates a plurality of charged microspheres, and surfaces of the charged microspheres have a first biomolecule for specifically binding with a second biomolecule that enters the bio-detection chip so as to give rise to a color change. The charged microspheres move close to the upper substrate when a voltage is applied between the reference electrode and the driving electrode.
    Type: Grant
    Filed: April 23, 2018
    Date of Patent: July 20, 2021
    Assignees: BEIJING BOE OPTOELECTRONICS TECHNOLOGY CO., LTD., BOE TECHNOLOGY GROUP CO., LTD.
    Inventors: Yue Geng, Peizhi Cai, Fengchun Pang, Le Gu
  • Patent number: 11040343
    Abstract: This disclosure provides for devices and methods for conducting assays for combinatorial libraries. The devices comprise a multiplicity of wells and a removable cap.
    Type: Grant
    Filed: January 28, 2020
    Date of Patent: June 22, 2021
    Assignee: Plexium, Inc.
    Inventors: Kapil Mahakalkar, Kenneth Chung, Jesse Lu, Edgar Gutierrez, Yi Zhang
  • Patent number: 10987674
    Abstract: An apparatus includes a substrate, a first heating element, and a second heating element. The substrate includes a first portion, a second portion, and a third portion that is between the first portion and the second portion. The first portion is characterized by a first thermal conductivity, the second portion is characterized by a second thermal conductivity, and the third portion is characterized by a third thermal conductivity. The third thermal conductivity is less than the first thermal conductivity and the second thermal conductivity. The first heating element is coupled to the first portion of the substrate, and is configured to produce a first thermal output. The second heating element is coupled to the second portion of the substrate, and configured to produce a second thermal output. The second thermal output is different from the first thermal output.
    Type: Grant
    Filed: April 21, 2017
    Date of Patent: April 27, 2021
    Assignee: Visby Medical, Inc.
    Inventors: Boris Andreyev, Brian Ciopyk, Victor Briones, Jonathan Hong, David Swenson, Gregory Loney, Adam De La Zerda, Steven Chu
  • Patent number: 10946133
    Abstract: The present disclosure describes a system and method for microfluidic separation. More particularly, the disclosure describes a system and method for the purification of a fluid by the removal of undesired particles. The device includes microfluidic separation channels that include multiple outlets. The device also includes isolation slots positioned between each of the microfluidic separation channels. The device's base includes multiple acoustic transducers which in some implementations are configured to protrude into the isolation slots. The acoustic transducers are configured to generate aggregation axes within the separation channels, which are used to separate out undesired particles.
    Type: Grant
    Filed: April 25, 2018
    Date of Patent: March 16, 2021
    Assignee: THE CHARLES STARK DRAPER LABORATORY, INC.
    Inventors: Jason Oliver Fiering, Hoi-Cheong Steve Sun
  • Patent number: 10934596
    Abstract: A method for automated microbial detection includes collecting air particles into a solid-state sampler, the air particles including microbes, charging the air particles using a plasma field generated by propulsion electrodes, focusing the charged air particles toward a sample well of a microfluidic testing cartridge, tagging the charged air particles with a fluorescence marker, and detecting a quantity of the microbes using a fluorescence detector.
    Type: Grant
    Filed: April 10, 2018
    Date of Patent: March 2, 2021
    Assignee: UChicago Argonne, LLC
    Inventors: Jack Gilbert, Charles Catlett, Peter Beckman
  • Patent number: 10913066
    Abstract: A microfluidic diagnostic chip may comprise a microfluidic channel, a functionalizable enzymatic sensor in the microfluidic channel, the functionalizable enzymatic sensor comprising a binding surface to bind with a biomarker in a fluid, and a microfluidic pump to pass the fluid over the binding surface. A microfluidic device may comprise a number of pumps to pump a fluid though the number of microfluidic channels and a number of microfluidic channels comprising at least one sensor to detect a change in a chemical characteristic of the fluid in response to presence of the fluid on the sensor.
    Type: Grant
    Filed: January 30, 2015
    Date of Patent: February 9, 2021
    Assignee: Hewlett-Packard Development Company, L.P.
    Inventors: Manish Giri, Chantelle Elizabeth Domingue, Nicholas Matthew Cooper McGuinness, Jeremy Sells, Sirena Lu, Melinda M. Valencia
  • Patent number: 10895570
    Abstract: The invention relates to a method for determining the result of an agglutination reaction and a microplate used in such a method. Agglutinated and non-agglutinated sample material is separated by means of a separation material such as gel material or a bead matrix in a centrifugation step. Whether an agglutination reaction took place or not is determined by comparing the color intensities and/or the gray levels of images of the top side and the bottom side of the respective reaction vessel. Comparing the color intensities and/or the gray levels of the two images makes the automatic determination reliable and stable. Furthermore, the reaction wells are arranged in a two-dimensional array which provides a high throughput.
    Type: Grant
    Filed: October 7, 2014
    Date of Patent: January 19, 2021
    Assignee: Yantai AusBio Laboratories Co., Ltd.
    Inventors: Zhaoqiang Wang, Wolfgang Mann
  • Patent number: 10864522
    Abstract: In one embodiment, a multiplex fluid processing cartridge includes a sample well, a deformable fluid chamber, a mixing well with a mixer disposed therein, a lysis chamber including a lysis mixer, an electrowetting grid for microdroplet manipulation, and electrosensor arrays configured to detect analytes of interest. An instrument for processing the cartridge is configured to receive the cartridge and to selectively apply thermal energy, magnetic force, and electrical connections to one or more discrete locations on the cartridge and is further configured to compress the deformable chamber(s) in a specified sequence.
    Type: Grant
    Filed: August 15, 2019
    Date of Patent: December 15, 2020
    Assignee: GenMark Diagnostics, Inc.
    Inventors: Scott Corey, Brian Murphy, Eric Schneider, David Walter Wright, Dominic Aiello, Andrew Fish, Jay Srinivasan, George Maltezos
  • Patent number: 10866188
    Abstract: A fingermark lifting and visualization device includes a carrier and an indicator. The carrier has a lifting surface. The indicator is immobilized on the lifting surface. The indicator changes color or changes in fluorescence in the visible spectrum in response to contact with a glandular secretion.
    Type: Grant
    Filed: April 4, 2018
    Date of Patent: December 15, 2020
    Assignee: Board of Trustees of Northern Illinois University
    Inventors: Oliver D. Hofstetter, Andrei Mlakar, Claude Roux, Xanthe Spindler, Chris Lennard
  • Patent number: 10845305
    Abstract: An analyte-sensitive substance is provided that has an optical property related to the concentration of an analyte. The analyte-sensitive substance includes an ionophore or other substance configured to provide a local pH, within the analyte-sensitive substance, that is related to the concentration of the analyte proximate the analyte-sensitive substance. The analyte-sensitive substance further includes a pH-sensitive fluorophore that increases or decreases its intrinsic fluorescence intensity with the local pH across a specified range of pH values. The analyte-sensitive substance further includes a pH-sensitive quencher configured to increase the slope of the change of fluorescence intensity of the pH-sensitive fluorophore across the specified range of pH values. The analyte-sensitive substance may further include an ionic additive configured to adjust the local pH such that the specified range of pH values corresponds to a range of analyte concentration values of interest.
    Type: Grant
    Filed: November 20, 2015
    Date of Patent: November 24, 2020
    Assignee: Verily Life Sciences LLC
    Inventors: Timothy Tordella Ruckh, Zhan Wang
  • Patent number: 10837011
    Abstract: In a method of nucleic acid extraction using a support, the present invention realizes collection of a nucleic acid from a liquid containing the nucleic acid at an extremely low concentration at which collection cannot be achieved with polyacrylamide. A method of extracting a nucleic acid, comprising allowing the nucleic acid to be adsorbed onto a support in the presence of a chaotropic salt and an alcohol in coexistence with an anionic polymer. A reagent for nucleic acid extraction, comprising an anionic polymer, a chaotropic salt, an alcohol and a support.
    Type: Grant
    Filed: March 18, 2015
    Date of Patent: November 17, 2020
    Assignee: UNIVERSAL BIO RESEARCH CO., LTD.
    Inventors: Ryouji Karinaga, Tetsuya Ueda, Hideji Tajima
  • Patent number: 10828641
    Abstract: The present invention relates to systems and methods for the arrangement of droplets in pre-determined locations. Many applications require the collection of time-resolved data. Examples include the screening of cells based on their growth characteristics or the observation of enzymatic reactions. The present invention provides a tool and related techniques which addresses this need, and which can be used in many other situations. The invention provides, in one aspect, a tool that allows for stable storage and indexing of individual droplets. The invention can interface not only with microfluidic/microscale equipment, but with macroscopic equipment to allow for the easy injection of liquids and extraction of sample droplets, etc.
    Type: Grant
    Filed: April 25, 2017
    Date of Patent: November 10, 2020
    Assignee: President and Fellows of Harvard College
    Inventors: Christian Boehm, Amy Rowat, Sarah Koester, Jeremy Agresti, David A. Weitz
  • Patent number: 10807089
    Abstract: A fluidic device including an inorganic solid support attached to an organic solid support by a bonding layer, wherein the inorganic solid support has a rigid structure and wherein the bonding layer includes a material that absorbs radiation at a wavelength that is transmitted by the inorganic solid support or the organic solid support; and a channel formed by the inorganic solid support and the organic solid support, wherein the bonding layer that attaches the inorganic solid support to the organic solid support provides a seal against liquid flow. Methods for making fluidic devices, such as this, are also provided.
    Type: Grant
    Filed: February 22, 2018
    Date of Patent: October 20, 2020
    Assignee: ILLUMINA, INC.
    Inventors: Jeffrey S. Fisher, John A. Moon, Bala Murali Venkatesan
  • Patent number: 10794913
    Abstract: A single disposable cartridge for performing a process on a particle, such as particle sorting, encapsulates all fluid contact surfaces in the cartridge for use with microfluidic particle processing technology. The cartridge interfaces with an operating system for effecting particle processing. The encapsulation of the fluid contact surfaces insures, improves or promotes operator isolation and/or product isolation. The cartridge may employ any suitable technique for processing particles.
    Type: Grant
    Filed: March 4, 2019
    Date of Patent: October 6, 2020
    Assignee: CYTONOME/ST, LLC
    Inventors: John R. Gilbert, Hugh Lewis, Derek Beaupre, Jaishree Trikha, Manish Deshpande
  • Patent number: 10774300
    Abstract: The present invention is directed to methods and kits for separating, accumulating, characterizing and/or identifying microorganisms known to be present or that may be present in a test sample. The method of the invention comprises optional lysing non-microorganism cells and/or particulates that may be present in a test sample, followed by a subsequent filtration step for isolation and/or accumulation of microorganisms. The kit of the present invention may comprise at least one filter membrane or an integrated filtration and sample transfer device for isolation and/or accumulation of microorganisms.
    Type: Grant
    Filed: July 20, 2012
    Date of Patent: September 15, 2020
    Assignee: bioMerieux, Inc.
    Inventors: Jones M. Hyman, John D. Walsh, Ronnie J. Robinson, Christopher Ronsick
  • Patent number: 10753954
    Abstract: An apparatus for a vacuum-driven microfluidic probe includes a body with an apex and a processing surface, at an end of the body. The apparatus also includes a partially open cavity formed as a recess on the processing surface and a set of apertures in the cavity, where the set of apertures include a sample outlet aperture intersected by a vertical axis of the cavity. The apparatus also includes aspiration apertures radially distributed around said vertical axis, wherein the apex is further configured to generate a pressure in the cavity upon aspirating an external liquid through the aspiration apertures that causes to aspirate a liquid sample from the sample outlet aperture, so as to eject the aspirated liquid sample from the probe.
    Type: Grant
    Filed: July 11, 2017
    Date of Patent: August 25, 2020
    Assignee: International Business Machines Corporation
    Inventors: Govind Kaigala, Seyed Ali Kazemi Oskooei
  • Patent number: 10745686
    Abstract: The present invention provides method for isolating DNA molecules having a size above a certain cut-off value from a DNA containing sample, comprising a) contacting the sample with a binding buffer which comprises a chaotropic agent and a buffering agent to provide a binding mixture and binding DNA molecules having a size above the cut-off value to a binding matrix which has a silicon containing surface, wherein the cut-off value is determined by the pH value of the binding mixture; b) separating the bound DNA from the remaining sample; c) optionally washing the bound DNA; and d) optionally eluting the bound DNA from the binding matrix. Said method allows the size selective purification of DNA molecules.
    Type: Grant
    Filed: February 7, 2014
    Date of Patent: August 18, 2020
    Assignee: QIAGEN GmBH
    Inventors: Tanya Sperling, Nicola Scholle, Thorsten Singer, Margit Hiesinger
  • Patent number: 10732177
    Abstract: Compositions, methods, and devices for the detection of multiple analytes with a single signal are provided.
    Type: Grant
    Filed: November 1, 2017
    Date of Patent: August 4, 2020
    Assignee: INVISIBLE SENTINEL, INC.
    Inventors: Nicholas Siciliano, Louis Leong, Martin Patrick Keough, Ashley Shaniece Brown
  • Patent number: 10732649
    Abstract: The present invention provides microfluidic technology enabling rapid and economical manipulation of reactions on the femtoliter to microliter scale.
    Type: Grant
    Filed: June 30, 2017
    Date of Patent: August 4, 2020
    Assignee: The University of Chicago
    Inventors: Rustem F. Ismagilov, Bo Zheng, Cory John Gerdts
  • Patent number: 10725541
    Abstract: Systems and methods for forming a fluidic device using a layered construction strategy may include (1) forming a first component for the fluidic device out of a first material, (2) forming a second component for the fluidic device out of a second material that is different than the first material, and (3) after forming the first and second components, forming the fluidic device by assembling a variety of components including the first component and the second component.
    Type: Grant
    Filed: May 31, 2018
    Date of Patent: July 28, 2020
    Assignee: Facebook Technologies, LLC
    Inventors: Riccardo Desalvo, Matthew Schwab, Joseph Minh Tien, Jack Lindsay, Serol Turkyilmaz
  • Patent number: 10718759
    Abstract: Disclosed herein are a transparent immunoassay apparatus and method. The transparent immunoassay apparatus includes a sample pad configured to allow a liquid specimen to be dropped thereonto, a first porous pipe configured to move the dropped specimen in a lateral flow manner, an immunobinding reaction unit configured to generate a detectable signal from binding of a previously applied reactant and the specimen between transparent upper and lower plates of the immunobinding reaction unit, a second porous pipe configured to move the specimen, having passed through the immunobinding reaction unit, in a lateral flow manner, and an absorption pad configured to absorb the specimen transferred from the second porous pipe.
    Type: Grant
    Filed: June 5, 2017
    Date of Patent: July 21, 2020
    Assignee: ELECTRONICS AND TELECOMMUNICATIONS RESEARCH INSTITUTE
    Inventors: Yo-Han Choi, Ki-Bong Song
  • Patent number: 10705012
    Abstract: The invention is related to a device and method for analysing and controlling cell motility. In accordance with an aspect of the present invention, there if provided a microfluidic device for analyzing and controlling the motility of a cell, the device comprising: (a) a first inlet for introducing a cell sample; (b) an outlet for discharging the cell sample; (c) a microfluidic channel in fluid communication with and intermediate the first inlet and outlet; (d) a first pump coupled to the first inlet for pumping the cell sample in the microfluidic channel; and (d) an observation area within a portion of the microfluidic channel for analysing and controlling the motility of the cell, wherein the first pump generates a shear stress in the observation area, the shear stress generates a shearotactical signal for driving movement of the cell.
    Type: Grant
    Filed: April 22, 2016
    Date of Patent: July 7, 2020
    Inventors: Roland Bouffanais, Xiaoying Zhu, Dick K. P. Yue
  • Patent number: 10688493
    Abstract: A device for performing a microfluidic assay on a chip comprising, a microfluidics chip, one or more fluid receptacles on the chip for receiving a fluid, a plurality of pneumatic pumps arrayed on the chip, each pump having a discharge channel leading to a rectifier on the chip, and a reaction chamber in fluid communication with each of the rectifiers, wherein a pressure on the pressurized fluid source drives fluid from the fluid receptacle into the incoming fluid channel connecting the fluid receptacle to the pump, through the pump and into the discharge channel, through the discharge channel to the rectifier, and through the rectifier into the reaction chamber, wherein the pump is configured to generate droplets of a pre-determined size, wherein the rectifiers prevent backflow of the droplets, and wherein droplets are combined in the reaction chamber, the chamber facilitating an assay being performed on the chip.
    Type: Grant
    Filed: March 9, 2017
    Date of Patent: June 23, 2020
    Assignee: Texas Tech University System
    Inventors: Jungkyu Kim, Vladimir Coltisor
  • Patent number: 10683477
    Abstract: The present invention provides a device for producing a large number of uniform spheroids by an easy method. The spheroid-producing device (1) at least includes a first surface (11), a second surface (12), and a plurality of wall surfaces (13). The second surface (12) faces the first surface (11). The respective wall surfaces (13) constitute a plurality of holes penetrating through the first surface and the second surface. In addition, an equivalent diameter of inscribed circles of openings in the first surface (11) is greater than an equivalent diameter of inscribed circles of openings in the second surface (12).
    Type: Grant
    Filed: February 25, 2015
    Date of Patent: June 16, 2020
    Assignee: Corning Incorporated
    Inventor: Shoichiro Sumi
  • Patent number: 10646865
    Abstract: Sample manipulating tools may comprise a handle portion and a tool portion. A sample spreading tool may comprise a head connected to the handle portion by a pivot connection permitting at least pivotal movement of the tool portion with respect to the handle portion. The head may comprise a perimeter section and a bridge section. Another sample spreading tool may comprise a central element and at least two loop segments connected to the central element with each loop segment having a substantially arcuate shape. A sample picking tool may have a loop section forming a reservoir for receiving a liquid sample and a hook portion fixed to the loop section, with a first end fixed to the loop portion and a second end being a free end. A sample scraping tool may comprise a mounting structure to receive a pipette and a scraper structure having a scraping edge.
    Type: Grant
    Filed: March 7, 2018
    Date of Patent: May 12, 2020
    Inventor: John L. Sternick
  • Patent number: 10634672
    Abstract: A device is provided. The device comprises a casing comprising an interior, a first opening, and a second opening; and a porous carrier comprising a sample-receiving zone and a target cell-binding zone. The porous carrier defines a first fluid pathway that extends from the sample-receiving zone to the target cell-binding zone. At least portion of the porous carrier is disposed in the interior of the casing. A second fluid pathway comprising a central axis extends through the casing from the first opening and the second opening, the second fluid pathway intersecting the porous carrier at the target cell-binding zone. The central axis is oriented orthogonally with respect to the porous carrier. Methods of using the device to detect a target microorganism are also provided.
    Type: Grant
    Filed: October 5, 2015
    Date of Patent: April 28, 2020
    Assignee: 3M INNOVATIVE PROPERTIES COMPANY
    Inventors: Neil Percy, Gregory W. Sitton
  • Patent number: 10618047
    Abstract: This disclosure relates to an apparatus for simultaneously filling a plurality of sample chambers. In one aspect, the apparatus comprises a common fluid source and a plurality of independent, continuous fluidic pathways. Each independent, continuous fluidic pathway comprises a sample chamber and a pneumatic compartment. The sample chamber is connected to the common fluid source, and the pneumatic compartment is connected to the sample chamber. The sample chamber comprises, in part, an assay chamber. The assay chamber comprises a monolithic substrate and a plug. In some embodiments, the assay chamber contains a magnetic mixing element. In some embodiments, the assay chamber is a double tapered chamber. In some embodiments, a ratio of a volume of the sample chamber to a volume of the pneumatic compartment is substantially equivalent for each fluidic pathway of the plurality of fluidic pathways.
    Type: Grant
    Filed: July 5, 2018
    Date of Patent: April 14, 2020
    Assignee: Talis Biomedical Corporation
    Inventor: Thomas H. Cauley, III
  • Patent number: 10598668
    Abstract: Coating materials such as MALDI matrix solutions are aerosolized and are used to coat analyte particles in an acoustic coater. Methods and devices for coating analyte particles in real time are disclosed. The coating improves the detection and quantification of the analyte particles using analytical instruments such as an aerosol time of flight mass spectrometer.
    Type: Grant
    Filed: August 24, 2016
    Date of Patent: March 24, 2020
    Assignee: Zeteo Tech, Inc.
    Inventors: Wayne A. Bryden, Charles J. Call
  • Patent number: 10564171
    Abstract: A microfluidic diagnostic chip may comprise a substrate, a plurality of fluidic slots extending through the substrate, a plurality of microfluidic channels each coupled to a respective one of the plurality of fluidic slots to receive a plurality of fluids from the plurality of fluidic slots, and a mixing region in fluid communication with the plurality of fluidic slots to receive the plurality of fluids such that the plurality of fluids are to mix. A diagnostic chip may comprise a number of fluid slots defined through a substrate and a plurality of microfluidic channels coupled to the fluid slots to receive from the fluid slots a plurality of different fluids wherein the microfluidic channels combine and mix the plurality of different fluids.
    Type: Grant
    Filed: January 30, 2015
    Date of Patent: February 18, 2020
    Assignee: Hewlett-Packard Development Company, L.P.
    Inventors: Nicholas Matthew Cooper McGuinness, Chantelle Elizabeth Domingue, Manish Giri, Ed Friesen
  • Patent number: 10564121
    Abstract: Disclosed herein is a device for separation and analysis of trace and ultra-trace ionogenic compounds by isotachophoresis-zone electrophoresis on a chip with online detection and method of its use, concentrating trace and ultra-trace analytes by isotachophoretic migration in a wide separation channel when a manifold of auxiliary electrodes is employed. Then the isotachophoretic zones of trace and ultra-trace analytes are transferred by isotachophoretic migration through a tapered channel, while corresponding auxiliary electrodes are progressively disconnected from the power supply. When the isotachophoretic zones enter the analytical capillary channel, the mode switches to zone electrophoresis and an online detector detects analytes for qualitative and quantitative analysis.
    Type: Grant
    Filed: November 26, 2015
    Date of Patent: February 18, 2020
    Inventor: Vladislav Dolnik