Liquid Chromatography Patents (Class 422/70)
  • Patent number: 10466040
    Abstract: A sensor device (100), for measuring a surface (101), includes a lighting device (103) emitting a light beam (105); an optical device (107) splitting the light beam (105) into partial light beams (109, 111), and emitting the first partial light beam (109) toward a first surface area (113) and emitting the second partial light beam (111) toward a second surface area (115). A light sensor (117) is configured to receive a first surface area reflection (109-1) of the first partial light beam (109) and a second surface area reflection (111-1) of the second partial light beam (111). A processor (119) is configured to detect a distance of the first surface area (113) and of the second surface area (115) to the sensor device (100) based on a position of the first partial light beam reflection (109-1) and the second partial light beam reflection (111-1) on the light sensor (117).
    Type: Grant
    Filed: May 25, 2017
    Date of Patent: November 5, 2019
    Assignee: BAUMER ELECTRIC AG
    Inventors: Klaus Friedrich Müller, Sven Bauer
  • Patent number: 10465853
    Abstract: A system can include a valve assembly including a first valve and a second valve in fluid communication with the first valve. The valve assembly can be configured to deliver one or more of a sample, a chemical (e.g., an acid, a base, an organic chemical, etc.), and a standard via flow of a working fluid facilitated by one or more syringe pumps. Further, the one or more of the sample, the chemical, and the standard can maintain a physical separation from the one or more syringe pumps during delivery of the one or more of the sample, the chemical, and the standard.
    Type: Grant
    Filed: June 12, 2017
    Date of Patent: November 5, 2019
    Assignee: ELEMENTAL SCIENTIFIC, INC.
    Inventors: Daniel R. Wiederin, Austin Schultz
  • Patent number: 10453664
    Abstract: A unique fiber core sampler composition, related systems, and techniques for designing, making, and using the same are described. The sampler is used to interface with existing field instrumentation, such as Ion Mobility Spectrometer (IMS) equipment. Desired sampler characteristics include its: stiffness/flexibility; thermal mass and conductivity; specific heat; trace substance collection/release dependability, sensitivity and repeatability; thickness; reusability; durability; stability for thermal cleaning; and the like. In one form the sampler has a glass fiber core with a thickness less than 0.3 millimeter that is coated with a polymer including one or more of: polymeric organofluorine, polyimide, polyamide, PolyBenzlmidazole (PBI), PolyDiMethylSiloxane (PDMS), sulfonated tetrafluoroethylene (PFSA) and Poly(2,6-diphenyl-p-phenylene Oxide) (PPPO).
    Type: Grant
    Filed: April 21, 2015
    Date of Patent: October 22, 2019
    Assignee: Battelle Memorial Institute
    Inventors: Raymond S Addleman, Xiaohong Shari Li, Wilaiwan Chouyyok, David A Atkinson
  • Patent number: 10451592
    Abstract: An arrangement for preparing samples and analyzing pesticides in samples contains an HILIC chromatography column with a first pump for a predominately low-water and/or non-polar solvent; and SPE enrichment arrangement; a second chromatography column with a second pump for a predominantly water-rich and/or polar solvent; a detector; and a valve arrangement for controlling the stream of sample and matrix, which valve arrangement is designed in such a way that the sample stream, in a first switching state of the valve arrangement, can be conducted from the HILIC chromatography column to the SPE enrichment arrangement and, in a second switching state, the sample enriched in the SPE enrichment arrangement can be conducted in the opposite direction from the SPE enrichment arrangement through the second chromatography column to the detector.
    Type: Grant
    Filed: January 30, 2013
    Date of Patent: October 22, 2019
    Assignee: Joint Analytical Systems GmbH
    Inventors: Stefan Kittlaus, Jorg Radtke
  • Patent number: 10309940
    Abstract: There are provided a data processing device for chromatograph and a data processing method for chromatograph which allow a peak to be desirably checked. A peak (correction target peak (P1)) whose intensity exceeds a predetermined threshold in a chromatogram at a target wavelength (?1) is corrected based on correction reference values (height (H1) and area (A1) of a peak (P11)) and a sensitivity coefficient (R=I1/I2), and the chromatogram after correction is displayed or printed. Therefore, even if the correction target peak (P1) is saturated, display or printing may be performed in a state where correction has been performed so that the chromatogram at the peak (P1) is not cut off in the middle. Accordingly, at the time of display or printing of the chromatogram, a fine peak may be prevented from becoming too small, and also the correction target peak (P1) may be prevented from being cut off in the middle, and thus the peaks may be desirably checked.
    Type: Grant
    Filed: August 5, 2013
    Date of Patent: June 4, 2019
    Assignee: SHIMADZU CORPORATION
    Inventors: Etsuho Kamata, Toshinobu Yanagisawa, Yasuhiro Mito, Kenichi Mishima
  • Patent number: 10151734
    Abstract: A data processing system for a chromatograph including a standard sample data storage section; a standard sample sensitivity factor calculator; a post-correction standard sample chromatogram strength calculator; a specific designated retention time and specific designated wavelength setter; a measurement sample data storage section; a measurement sample sensitivity factor calculator; and a post-correction measurement sample chromatogram creator.
    Type: Grant
    Filed: September 2, 2013
    Date of Patent: December 11, 2018
    Assignee: SHIMADZU CORPORATION
    Inventors: Etsuho Kamata, Toshinobu Yanagisawa, Yasuhiro Mito, Kenichi Mishima
  • Patent number: 10099158
    Abstract: An apparatus and a method are provided for column chromatography which provide improvements in separation resolution and detection sensitivity, comprising a chromatography column, the column having an inlet and an outlet, wherein the outlet is configured to split a flow of eluate as it leaves the column through the outlet into at least two separate portions, wherein the apparatus is configured to separately process the portions, for example to separately detect a portion or separately collect fractions of a portion with improved resolution. A split frit assembly is preferably configured to split the flow of eluate. The portions preferably emanate from different radial regions of the column. An end fitting for the column outlet may be provided having multiple ports to separately convey the portions.
    Type: Grant
    Filed: May 25, 2012
    Date of Patent: October 16, 2018
    Assignees: Thermo Electron Manufacturing Limited, University of Western Sydney
    Inventors: Harald Ritchie, Ross Andrew Shalliker
  • Patent number: 10090139
    Abstract: A mass analysis device capable of reliably detecting the peak in a mass chromatogram of a given m/z is equipped with a control unit, which generates a mass chromatogram and total ion chromatogram. The control unit includes a determination unit which, using the total ion chromatogram, determines the start time and end time of the peak in the total ion chromatogram by searching for the peak based on maximum value of detected intensity and searching for peak start time and end time based on slope of change of detected intensity; and a detection unit, which detects the peak in the mass chromatogram by making the start time and end time of the peak in the mass chromatogram the same as the start time and end time of the peak in the total ion chromatogram.
    Type: Grant
    Filed: October 15, 2014
    Date of Patent: October 2, 2018
    Assignee: SHIMADZU CORPORATION
    Inventor: Yutaro Yamamura
  • Patent number: 10018620
    Abstract: The present disclosure describes systems and methods for mimicking body tissue and the function thereof. The mimicked body tissue can include kidney tissue, the blood brain barrier, and other tissues. In some implementations, the systems described herein are used to test the impact of controlled factors on the tissue. The controlled factors can include flow rates, shear rates, and test chemicals (e.g., therapeutics and toxins). In some implementations, the system and methods are used to test pharmaceutical and biological therapies, characterize healthy or diseased tissue, and observe phenomena of the tissue in vitro.
    Type: Grant
    Filed: April 16, 2015
    Date of Patent: July 10, 2018
    Assignee: The Charles Stark Draper Laboratory, Inc.
    Inventors: Joseph L. Charest, Else Frohlich, Christopher DiBiasio, Kenneth Vandevoordt
  • Patent number: 9921192
    Abstract: The disclosure describes embodiments of an apparatus including a first gas chromatograph including a fluid inlet, a fluid outlet, and a first temperature control. A controller is coupled to the first temperature control and includes logic to apply a first temperature profile to the first temperature control to heat, cool, or both heat and cool the first gas chromatograph. Other embodiments are disclosed and claimed.
    Type: Grant
    Filed: March 16, 2015
    Date of Patent: March 20, 2018
    Assignee: TricornTech Corporation
    Inventors: Tsung-Kuan A. Chou, Shih-Chi Chu, Chia-Sheng Cheng, Li-Peng Wang, Chien-Lin Huang
  • Patent number: 9723298
    Abstract: A system and computer implemented method for managing a display for a software application is disclosed. The software application may have a set of portions. The method can include collecting, for a first portion and a second portion of the set of portions, optical tracking information including a set of viewing coordinates having a temporal feature. The method can also include determining, based on the optical tracking information including the set of viewing coordinates having the temporal feature, a set of viewing scores including a first group of viewing scores and a second group of viewing scores. The method can also include generating a frustum having a set of regions. The set of regions can include a first region, to provide the first portion, based on the first group of viewing scores, and a second region, to provide the second portion, based on the second group of viewing scores.
    Type: Grant
    Filed: June 13, 2014
    Date of Patent: August 1, 2017
    Assignee: International Business Machines Corporation
    Inventor: Santosh Devale
  • Patent number: 9697995
    Abstract: A method for analyzing a mixture of components includes forming precursor ions from the components, alternately causing the precursor ions to pass to and to by-pass a fragmentation device, to form product ions from the precursor ions that pass to the device and to form substantially fewer product ions from precursor ions that by-pass the device, and obtaining mass spectra from product ions received from the device and from precursor ions that by-passed the device. An apparatus for analyzing a sample includes an ion source for forming precursor ions from the components of the sample, a fragmentation device for forming product ions from the precursor ions, a by-pass device disposed upstream of the fragmentation device for switchable by-pass of the fragmentation device, and a mass analyzer.
    Type: Grant
    Filed: November 20, 2015
    Date of Patent: July 4, 2017
    Assignee: Micromass UK Limited
    Inventor: Robert Harold Bateman
  • Patent number: 9638706
    Abstract: An external standard solution for use in the analysis of amino acid in plasma, containing, (1) at least one amino acid selected from the following components A, at a concentration of 0.0007 M to 0.49 M, and (2) (i) at least one amino acid selected from the following components B, at a concentration of 0.2 to 0.9 times of the lowest-concentration amino acid among the amino acids selected from components A, (ii) at least one amino acid selected from the following components C, at a concentration of 0.1 to 0.4 times of the lowest-concentration amino acid among amino acids selected from the components A, or (iii) at least one amino acid selected from the following components D, at a concentration of 0.05 to 0.
    Type: Grant
    Filed: July 19, 2012
    Date of Patent: May 2, 2017
    Assignees: WAKO PURE CHEMICAL INDUSTRIES, LTD., AJINOMOTO CO., INC.
    Inventors: Masako Hayakawa, Kazutaka Shimbo, Hiroo Yoshida
  • Patent number: 9580736
    Abstract: Provided herein are methods and compositions for analyzing nucleic acids associated with single cells using nucleic acid barcodes. According to some embodiments, a method for producing one or more polynucleotides of interest comprises: obtaining a plurality of RNAs associated with one or more samples, wherein the samples are obtained from one or more subjects, each RNA is associated with a single sample, and the RNAs associated with each sample are present in a separate reaction volume; adding an adapter molecule to the RNAs associated with each sample, wherein the adapter molecule is generated using an enzymatic reaction and comprises a universal priming sequence, a barcode sequence, and a binding site; and incorporating the barcode sequence into one or more polynucleotides associated with each sample, thereby producing the one or more polynucleotides of interest.
    Type: Grant
    Filed: December 30, 2014
    Date of Patent: February 28, 2017
    Assignee: Atreca, Inc.
    Inventors: Yann Chong Tan, Gary Withey
  • Patent number: 9573099
    Abstract: The present invention generally relates to emulsions, and more particularly, to double and other multiple emulsions. Certain aspects of the present invention are generally directed to the creation of double emulsions and other multiple emulsions at a common junction of microfluidic channels. In some cases, the microfluidic channels at the common junction may have substantially the same hydrophobicity. In one set of embodiments, a device may include a common junction of six or more channels, where a first fluid flows through one channel, a second fluid flows through two channels, and a third or carrying fluid flows through two more channels, such that a double emulsion of a first droplet of the first fluid, contained in a second droplet of the second fluid, contained by the carrying fluid, flows away from the common junction through a sixth channel.
    Type: Grant
    Filed: December 7, 2015
    Date of Patent: February 21, 2017
    Assignee: President and Fellows of Harvard College
    Inventors: David A. Weitz, Assaf Rotem, Adam R. Abate, Christian Holtze
  • Patent number: 9482651
    Abstract: The present invention relates to a method for producing a miniaturized separation column for chromatographic purposes including a porous stationary phase anchored in the column, including the following steps: (a) preparing a flat substrate of silicon, glass, glass ceramic or ceramic; (b) etching at least one channel structure into the flat substrate; (c) introducing a non-porous precursor material for the porous stationary phase into at least one portion of the channel structure (s); (d) forming a porous, three-dimensional network from the precursor material; and (e) fluid-tight covering of the channel structure(s) on the top side of the flat substrate.
    Type: Grant
    Filed: November 5, 2010
    Date of Patent: November 1, 2016
    Assignee: Fraunhofer-Gesellschaft Zur Foerderung Der Angewandten Forschung E.V.
    Inventors: Gundula Piechotta, Hans-Joachim Quenzer
  • Patent number: 9347931
    Abstract: The present invention is directed to a lateral flow assay device for the monitoring and measuring of coagulation and method thereof. Ideally, the invention is directed to a lateral capillary flow device for the monitoring and/or measurement of coagulation in a liquid sample wherein the device comprises a non-porous substrate with a zone for receiving a sample and a defined flow path zone wherein a clotting agent is deposited on at least part of the defined flow path zone to accelerate the coagulation of the liquid sample, enable the formation of an evenly distributed clot along the defined flow path zone and to result in the change in flow rate or cessation of flow of the liquid sample along the defined flow path zone.
    Type: Grant
    Filed: April 23, 2010
    Date of Patent: May 24, 2016
    Assignees: DUBLIN CITY UNIVERSITY, ÅMIC AB
    Inventors: Anthony Joseph Killard, Magdalena Maria Dudek, Brian MacCraith, Ib Mendel-Hartvig, Ove Öhman
  • Patent number: 9238206
    Abstract: The present invention generally relates to emulsions, and more particularly, to double and other multiple emulsions. Certain aspects of the present invention are generally directed to the creation of double emulsions and other multiple emulsions at a common junction of microfluidic channels. In some cases, the microfluidic channels at the common junction may have substantially the same hydrophobicity. In one set of embodiments, a device may include a common junction of six or more channels, where a first fluid flows through one channel, a second fluid flows through two channels, and a third or carrying fluid flows through two more channels, such that a double emulsion of a first droplet of the first fluid, contained in a second droplet of the second fluid, contained by the carrying fluid, flows away from the common junction through a sixth channel.
    Type: Grant
    Filed: May 22, 2012
    Date of Patent: January 19, 2016
    Assignees: President and Fellows of Harvard College, BASF SE
    Inventors: Assaf Rotem, David A. Weitz, Adam R. Abate, Christian Holtze
  • Patent number: 9217733
    Abstract: A method and system for detecting residual poison in human body are provided. Using the disclosed HPLC-Chip-mass spectrometry (MS)/MS and/or HPLC-MS/MS method to detect the residual poison, the method of the present invention mainly includes sample preparation, liquid chromatography and mass spectrometry. The method of the present invention has advantages of low sample size, high specificity, low detection limit, high sensitivity, low cost, high accuracy and stability, etc.
    Type: Grant
    Filed: November 5, 2010
    Date of Patent: December 22, 2015
    Assignee: The Hong Kong University of Science and Technology
    Inventors: Karl Wah Keung Tsim, Tina Tingxia Dong, Kevin Yue Zhu, Ka Wing Leung, Tiejie Wang
  • Patent number: 9029162
    Abstract: Disclosed are methods and systems for the analysis of testosterone in a sample using supported liquid extraction and liquid chromatography-mass spectrometry.
    Type: Grant
    Filed: February 7, 2012
    Date of Patent: May 12, 2015
    Assignee: Laboratory Corporation of America Holdings
    Inventors: Russell Philip Grant, Matthew Crawford, Donald Walt Chandler, William Curtin
  • Publication number: 20150118675
    Abstract: The present invention provides an immunochromatography detection method capable of suppressing non-specific reactions. The present invention relates to an immunochromatography detection method including: a step of adding an analyte dilution solution containing an analyte to a chromatography medium; a step of recognizing a detection target by a labeling substance modified with gold nanoparticles, which is dry-retained at a labeling substance retaining part; a step of developing a composite of the labeling substance and the detection target as a mobile phase; and a step of detecting the detection target in the developed mobile phase at a judgment part, wherein the labeling substance is protected with a polyalkylene glycol having one or more mercapto groups and/or a derivative thereof and then dry-retained together with arginine and casein at the labeling substance retaining part.
    Type: Application
    Filed: March 13, 2013
    Publication date: April 30, 2015
    Inventor: Daisuke Ito
  • Patent number: 9005526
    Abstract: A polymer-based PLOT capillary column prepared by in situ copolymerization of a functional monomer and a crosslinking monomer, which enhances the strength of the polymer matrix, is disclosed. Also disclosed is a system comprising the polymer-based PLOT column coupled to a mass flow or concentration sensitive detector, for carrying out a chemical analysis method on samples separated by liquid chromatography using the column, and a process for using the system. Columns of the invention can be prepared in a robust fashion with a very narrow i.d., e.g., 5-15 ?m. Thus, they are suitable for commercial use in ultratrace LC/MS proteomic analysis. Columns according to the invention are characterized by high resolving power and high column-to-column reproducibility. When these columns are coupled on-line with, e.g., ESI-MS detection, the resulting systems are capable of detecting the component parts of complex proteomic samples down to the low attomole to sub-attomole level.
    Type: Grant
    Filed: October 8, 2013
    Date of Patent: April 14, 2015
    Assignee: Northeastern University
    Inventors: Barry L. Karger, Jian Zhang
  • Patent number: 8999245
    Abstract: The disclosure describes a cascaded gas chromatograph including a first gas chromatograph having a first temperature control and a second gas chromatograph coupled to the first gas chromatograph. The first and second chromatographs have individual temperature controls that can be controlled independently of each other. Other embodiments are disclosed and claimed.
    Type: Grant
    Filed: July 6, 2010
    Date of Patent: April 7, 2015
    Assignee: Tricorn Tech Corporation
    Inventors: Li-Peng Wang, Chien-Lin Huang, Tsung-Kuan A. Chou
  • Patent number: 8985144
    Abstract: A plurality of suctioning flow paths for suctioning mobile phases meet each other at a meeting portion. The meeting portion is connected to an inlet flow path communicating with an inlet of a reciprocating pump. The suctioning flow paths are respectively provided with solenoid valves for opening and closing the respective flow paths. The suctioning flow paths are respectively provided with orifices between the solenoid valves and the meeting portion. The orifices are sections of the respective suctioning flow paths having smaller diameters.
    Type: Grant
    Filed: December 20, 2011
    Date of Patent: March 24, 2015
    Assignee: Shimadzu Corporation
    Inventors: Yoshiaki Aso, Fujio Inoue
  • Publication number: 20150079608
    Abstract: A method for detecting or quantifying an analyte, using a test strip for lateral flow type chromatography which contains a membrane and a detection part on which a capturing ligand that is to specifically bind to the analyte has been fixed on the membrane, includes: bringing an analyte contained in a sample into contact with a labeled ligand labeled with a phosphor that is to be excited by light having a wavelength from 600 nm to 800 nm to generate fluorescence, bringing a complex containing the analyte and the labeled ligand into contact with a capturing ligand at the detection part, and irradiating on the test strip light having a wavelength from 600 nm to 800 nm as an excitation light for the phosphor contained in the complex to generate fluorescence from the phosphor, and measuring a fluorescence intensity of the fluorescence.
    Type: Application
    Filed: April 2, 2013
    Publication date: March 19, 2015
    Applicants: ADTEC, INC., KONICA MINOLTA, INC.
    Inventors: Tsuruki Tamura, Katsuyoshi Takayama
  • Publication number: 20150079623
    Abstract: The apparatus for analyzing a disease sample according to the present invention includes: a member for separating and quantitatively determining an amino acid stereoisomer in a biological material from a subject; a member for obtaining a disease state index value through a calculation by substituting the amount of the amino acid stereoisomer into a discriminant equation; and a member for outputting disease state information on the subject on the basis of the disease state index value. The method for analyzing a disease sample according to the present invention includes: a step of measuring the amount of amino acid stereoisomers in a biological material from a subject; a step of obtaining a disease state index value through a calculation by substituting the amount of the amino acid stereoisomers into a discriminant equation; and the like.
    Type: Application
    Filed: March 18, 2013
    Publication date: March 19, 2015
    Inventors: Kenji Hamase, Yousuke Toujo, Masashi Mita, Chieko Mizumoto
  • Patent number: 8980572
    Abstract: A method for generating and localizing a signal in a solid phase substrate detection system comprises applying a solution of target material to a substrate; binding the target with a specific affinity molecule having an attached label, the label comprising multiple signal precursor molecules; applying a carrier to the substrate, and treating the label to convert the signal precursor molecules to signal generating molecules. The carrier comprises solvent for the label and thickener for localizing the signal. The carrier may include developer that converts signal precursor molecules to signal generating molecules. Developer is not necessary if the signal precursor molecules are converted to signal generating molecules by e.g. temperature change, pH change, sonication, light irradiation, microwave heating. A test device for detecting target in a fluid sample, and a kit of parts for determining the presence of target in a fluid sample are also disclosed.
    Type: Grant
    Filed: June 14, 2010
    Date of Patent: March 17, 2015
    Assignee: Supernova Diagnostics, Inc.
    Inventors: Ling Wai Wong, Pui Yee Cangel Chan, Wing Cheung Mak, King Keung Sin, Reinhard Renneberg
  • Patent number: 8968560
    Abstract: Methods and related apparatuses and mixtures are described for chromatographic analysis. The described system includes a pressurized source of a mobile phase and a flow path in fluid communication with the pressurized source such that the mobile phase flows through the flow path. The system also includes an injector in fluid communication with the flow path and downstream of the pressurized source, the injector being configured to inject a sample into the flow path. A first column located downstream of the injector, contains a stationary phase, and forms part of the flow path. A first detector is positioned to detect properties of fluid in the flow path at a location downstream of the injector and upstream from the first column. A second detector is positioned to detect properties of fluid in the flow path at a location downstream of the first column.
    Type: Grant
    Filed: December 6, 2007
    Date of Patent: March 3, 2015
    Assignee: Schlumberger Technology Corporation
    Inventor: William H. Steinecker
  • Publication number: 20150056688
    Abstract: Systems include test cells with sorbent material in a T-shape and defining a first flow path for a solution and a second flow path for a sample, and a test line or test site with immobilized antigens or antibodies or other ligand binding molecules located at the junction of the T. A housing houses the sorbent material and defines a first hole adjacent an end of the first flow path for receiving the solution and a second hole adjacent an end of the second flow path for receiving the sample.
    Type: Application
    Filed: November 4, 2014
    Publication date: February 26, 2015
    Applicant: CHEMBIO DIAGNOSTICS SYSTEMS, INC.
    Inventor: Javanbakhsh Esfandiari
  • Patent number: 8963077
    Abstract: Provided are methods for determining the amount of reverse T3 in a sample using mass spectrometry. The methods generally involve ionizing reverse T3 in a sample and detecting and quantifying the amount of the ion to determine the amount of reverse T3 in the sample.
    Type: Grant
    Filed: February 26, 2014
    Date of Patent: February 24, 2015
    Assignee: Quest Diagnostics Investments, Inc.
    Inventors: J. Fred Banks, Peter P. Chou, Noriya M. Matt
  • Patent number: 8961764
    Abstract: A DNA analyzer includes an interface for coupling a microfluidic chip to the DNA analyzer. The microfluidic chip includes a first separation channel for electrophoretic separation of DNA fragments in a first sample. Further, the DNA analyzer includes a first optical device. The first optical device includes an illuminating path and a detecting path. The illuminating path directs a first input light beam received from a light source to a first separation channel of the microfluidic chip. The first input light beam causes fluorescent labels attached on DNA fragments in the first separation channel to emit a first fluorescence light. The detecting path collects and directs the first fluorescent light to a first plurality of optical fibers. Further, the DNA analyzer includes a spectrometer configured to receive the first fluorescent light from the plurality of optical fibers and detect fluorescent components in the first fluorescent light.
    Type: Grant
    Filed: October 14, 2011
    Date of Patent: February 24, 2015
    Assignees: Lockheed Martin Corporation, ZyGEM Corporation, Ltd.
    Inventors: Peter Karl Trost, Michael E. Egan, Doug South, Brian E. Root, Orion N. Scott, James P. Landers
  • Patent number: 8959984
    Abstract: This invention relates to methods for the evaluation and/or quantification of the binding affinity of small molecules or other compounds to target components contained within an analyte, such as target proteins contained within the proteome of a cell or tissue.
    Type: Grant
    Filed: September 26, 2008
    Date of Patent: February 24, 2015
    Assignee: Max-Planck-Gesellschaft zur Forderung der Wissenschaften E.V.
    Inventors: Henrik Daub, Michaela Bairlein, Kirti Sharma, Klaus Godl, Andreas Tebbe, Christoph Weber
  • Publication number: 20150024514
    Abstract: The present invention relates to a method for determining analytes and to a device suitable for this purpose.
    Type: Application
    Filed: March 6, 2013
    Publication date: January 22, 2015
    Applicant: SECURETEC DETEKTIONS-SYSTEME AG
    Inventors: Torsten Stadthagen, Frank Schwieger, Sebastian Klaus
  • Patent number: 8932524
    Abstract: A stationary medium is employed both to separate chemicals from a sample solution and also to generate surface-enhanced Raman scattering, so that spectral analysis of the separated analyte chemical can be performed. Applied driving force causes the sample to flow into the stationary medium and to distribute therethrough, thereby causing rapid separation of the analyte chemical, and surface-enhanced Raman scattered radiation is quickly detected, at a plurality of locations along a flow path defined by the stationary medium, for ultimate analysis.
    Type: Grant
    Filed: September 2, 2011
    Date of Patent: January 13, 2015
    Assignee: Real-Time Analyzers, Inc.
    Inventors: Stuart Farquharson, Paul Maksymiuk, Chetan S. Shende
  • Publication number: 20150011016
    Abstract: A pipette tip device for use in dispersive SPE. The device includes a pipette tip having a lower barrier, loose sorbent that is freely moveable during the extraction process, and a baffle system that is shaped to disrupt the flow of liquid sample that is aspirated into the pipette tip. The baffle system includes an insert that may be separate from or monolithic with the interior of the pipette tip.
    Type: Application
    Filed: February 14, 2013
    Publication date: January 8, 2015
    Inventor: William E. Brewer
  • Patent number: 8920658
    Abstract: Methods and apparatus for desolvating flowing liquid streams while retaining temporal resolution of dissolved substrates are disclosed. A novel small-scale self-regulating spray dryer preserves temporal resolution while desolvating a liquid chromatography eluent stream and depositing the solute onto an optical surface for infrared spectrographic analysis. The liquid eluent is pumped through a heated nebulizer to create a high-speed jet of solute containing liquid and solvent vapor. This jet is directed circumferentially inside a hot cylindrical cavity. Centrifugal force causes the larger liquid droplets to travel along the outer diameter of the cavity. The cavity surface is heated to cause the droplets to film boil. Film boiling reduces droplet contact with the cavity surface thereby retaining the solute in the droplets. The solute temperature is limited by controlling the pressure into which the solvent evaporates from the droplets.
    Type: Grant
    Filed: February 21, 2014
    Date of Patent: December 30, 2014
    Assignee: Spectra Analysis Instruments, Inc.
    Inventors: William W. Carson, Sidney Bourne
  • Patent number: 8920719
    Abstract: Provided is a strip-assembled immunochromatographic disc, containing: a base, a lid engaged with the base and a draining piece disposed between the strips on the base and the lid, wherein a sampling opening is disposed on the lid directly facing to the draining piece, and the said sampling opening intercommunicates to a draining groove provided on the inner side of the lid which is formed by a plurality of draining channels; several strip stages are provided on the base with their location and number corresponding to those of the draining channels provided on the lid, and the edge of the draining piece laps to the sample pads of the strips carried on the stage adjacent to one end of the sampling opening. Also provided is a method of performing multiplexed immnochromatographic detection using the strip disc to accomplish the detection of multiple target analytes in one sample in an assay.
    Type: Grant
    Filed: April 23, 2007
    Date of Patent: December 30, 2014
    Assignee: Institute of Microbiology and Epidemiology, Acadamy of Military Medical Sciences
    Inventors: Lei Zhou, Zongmin Du, Ruifu Yang, Huijie Huang
  • Patent number: 8921113
    Abstract: A buffer kit includes a first eluent and second eluent. The first eluent solution includes at least four buffer salts where at least three of the four buffer salts are a monovalent buffer salt, have a net negative charge or a net neutral zwitterionic charge, and include a sulfonate group and an amine. The second eluent solution includes at least four buffer salts where at least three of the four buffer salts are a monovalent buffer salt, have a net negative charge or a net neutral zwitterionic charge, and include a sulfonate group and an amine. The first eluent solution has a first pH and the second eluent solution has a second pH where the first pH and second pH are different values. The buffer kit provides a linear pH gradient that forms an approximately straight line from at least the first pH to the second pH.
    Type: Grant
    Filed: December 21, 2012
    Date of Patent: December 30, 2014
    Assignee: Dionex Corporation
    Inventors: Shanhua Lin, Christopher A. Pohl
  • Publication number: 20140377878
    Abstract: The present invention provides a rack for holding sample vials comprising: (i) a solid base; (ii) an upwardly extending wall around the perimeter of said base; (iii) a plurality of means for supporting a plurality of vials, each means for supporting one vial; and (iv) a means for draining liquid from said rack.
    Type: Application
    Filed: December 14, 2012
    Publication date: December 25, 2014
    Inventors: Ross Oliver Beecroft, Victoria Louise Goff, Sarah Jane Karim, Nicholas Brian Milne
  • Patent number: 8905073
    Abstract: A microfluidic device provided with a micro-channel structure capable of easily and positively providing therein micro-droplet having various dilution ratios. A micro-channel structure provided in a substrate (2) has a first mixing unit (11) and a second mixing unit (21) connected to the downstream side of the first mixing unit (11), with each mixing unit (11, 21) having first through third micro-channels. One end of a first weighing unit (11d) consisting of a micro-channel having a capacity equivalent to the volume of a specified-amount first micro-droplet is opened to a first micro-channel (11a), and the other end is opened to a merging unit (12a) provided on a second micro-channel (12). One end of a second weighing unit (13d) consisting of a micro-channel having a capacity equivalent to the volume of a specified-amount second micro-droplet is connected to a third micro-channel (13), and the other end is opened to the merging unit (12a).
    Type: Grant
    Filed: March 8, 2007
    Date of Patent: December 9, 2014
    Assignee: Sekisui Chemical Co. Ltd.
    Inventors: Kazuki Yamamoto, Hiroji Fukui, Minoru Seki
  • Patent number: 8906229
    Abstract: The invention concerns a device comprising: a base (2); a moveable or removable door (20), said device having a closed door position; and in the closed door position, a circuit (8) comprising a bag comprising two flexible films and conveying network connectors, and a press (9) comprising a first shell (16) disposed on said front face (5) of said base (2) and a second shell (17) disposed in said door (20); said bag being clamped between said first shell (16) and said second shell (17) in a state in which conduits of said network for conveying liquid are formed between said films.
    Type: Grant
    Filed: May 26, 2011
    Date of Patent: December 9, 2014
    Assignee: EMD Millipore Corporation
    Inventors: Sebastien Cirou, Rene Reinbigler, Virginie Buisson, Jean-Louis Weissenbach
  • Patent number: 8906231
    Abstract: The present invention provides a method and automated system for the purification of polypeptides including the direct filtration of solutions containing the polypeptides after purification.
    Type: Grant
    Filed: September 10, 2010
    Date of Patent: December 9, 2014
    Assignee: Lonza Biologics PLC
    Inventors: Mark R. Whickman, Sam Mansoor
  • Patent number: 8906692
    Abstract: Disclosed are an apparatus and method for separation analysis of mannose-6-phosphate (M6P) by post-column fluorescence detection method. The apparatus is based on chromatography, and includes a column with a solid phase having affinity for phosphate, a flow path for the eluate, a heater installed on the flow path for M6P and a basic amino acid to react by heating the eluate in the flow path, and a fluorescence detector installed downstream of the heater for continuously irradiating the eluate with excitation light and measuring the intensity of the emission, and may include in the flow path a supply channel for addition of a basic amino acid between the column and the heater. The method is characterized in that it uses the apparatus and a second mobile phase consisting of a second buffer containing phosphate of predetermined concentration and adjusted to a predetermined pH.
    Type: Grant
    Filed: March 26, 2012
    Date of Patent: December 9, 2014
    Inventor: Tetsuo Yokoyama
  • Patent number: 8890061
    Abstract: Provided are methods for determining the amount of reverse T3 in a sample using mass spectrometry. The methods generally involve ionizing reverse T3 in a sample and detecting and quantifying the amount of the ion to determine the amount of reverse T3 in the sample.
    Type: Grant
    Filed: July 29, 2014
    Date of Patent: November 18, 2014
    Assignee: Quest Diagnostics Investments, Inc.
    Inventors: J. Fred Banks, Peter P. Chou, Noriya M. Matt
  • Patent number: 8889423
    Abstract: A method for analyzing a binding ability of protein to a compound, comprising the steps of (a) fractionating a first group of isotope-labeled proteins into plural fractions using a carrier having the compound immobilized thereon; (b) fractionating a second group of proteins into one or plural fractions using a carrier having the compound immobilized thereon; (c) adding an amount of at least one fraction obtained in step (b) to each of the fractions obtained in step (a); (d) analyzing the fractions obtained in step (c) with mass spectrometry; and (e) based on the mass spectrometry information, obtaining, regarding each fraction, an intensity ratio between a peak derived from a protein in the fraction obtained in step (a) and a peak derived from a protein in the fraction obtained in step (b), and comparing degrees of the binding ability of the plural kinds of proteins to the compound.
    Type: Grant
    Filed: July 31, 2006
    Date of Patent: November 18, 2014
    Assignee: Eisai R & D Management Co., Ltd.
    Inventors: Yoshiya Oda, Hiroyuki Katayama
  • Publication number: 20140273264
    Abstract: This invention provides mixed-mode stationary phase compositions, devices and systems comprising the stationary phases as well as methods of producing these compositions using epoxide ring-opening reactions. Also provided are methods of using the stationary phases of the invention in separations.
    Type: Application
    Filed: March 15, 2013
    Publication date: September 18, 2014
    Inventors: Xiaodong LIU, Liang CAO, Christopher A. POHL
  • Publication number: 20140248714
    Abstract: Methods and systems for processing samples fixed to a porous substrate generally comprising, a compressor defining one or more fluid isolation areas, a support, for the porous substrate, having an opening corresponding to one or more of the fluid isolation areas of the compressor, an actuator that causes at least a portion of the compressor to press against the porous substrate, a fluid inlet having access to the fluid isolation area at least when the compressor is pressed against the porous substrate, and a fluid outlet to receive fluid, through the opening in the support corresponding to the fluid isolation area of the compressor, at least when the compressor is pressed against the porous substrate.
    Type: Application
    Filed: March 10, 2014
    Publication date: September 4, 2014
    Applicant: WHATMAN INTERNATIONAL LIMITED
    Inventors: Philip Alexander Shoemaker, Weston Blaine Griffin, Erin Jean Finehout, Xuefeng Wang, Kashan Ali Shaikh, Greg Darryl Goddard
  • Patent number: 8802010
    Abstract: A chromatographic method including chromatographically separating sample ionic species in an eluent stream, detecting the separated sample ionic species, catalytically combining hydrogen and oxygen gases or catalytically decomposing hydrogen peroxide in a catalytic gas elimination chamber, and recycling the effluent stream from the chamber to the chromatography separation column. The residence time between the detector and the chamber is at least about one minute. Also, flowing the recycle sequentially through two detector effluent flow channels of an electrolytic membrane suppressor. Also, applying heat or UV energy between the detector and the chamber. Also, detecting bubbles after the chamber. Also, a Platinum group metal catalyst and ion exchange medium in the chamber. Apparatus for performing the methods.
    Type: Grant
    Filed: May 30, 2013
    Date of Patent: August 12, 2014
    Assignee: Dionex Corporation
    Inventors: Yan Liu, Kannan Srinivasan, Christopher A. Pohl, Sheetal Bhardwaj, Zhongqing Lu
  • Patent number: 8802011
    Abstract: A chromatographic method including chromatographically separating sample ionic species in an eluent stream, detecting the separated sample ionic species, catalytically combining hydrogen and oxygen gases or catalytically decomposing hydrogen peroxide in a catalytic gas elimination chamber, and recycling the effluent stream from the chamber to the chromatography separation column. The residence time between the detector and the chamber is at least about one minute. Also, flowing the recycle sequentially through two detector effluent flow channels of an electrolytic membrane suppressor. Also, applying heat or UV energy between the detector and the chamber. Also, detecting bubbles after the chamber. Also, a Platinum group metal catalyst and ion exchange medium in the chamber. Apparatus for performing the methods.
    Type: Grant
    Filed: May 30, 2013
    Date of Patent: August 12, 2014
    Assignee: Dionex Corporation
    Inventors: Yan Liu, Kannan Srinivasan, Christopher A. Pohl, Sheetal Bhardwaj, Zhongqing Lu
  • Publication number: 20140212981
    Abstract: Disclosed are methods and systems for the analysis of thiopurine drug metabolites in a sample using liquid chromatography/mass spectrometry.
    Type: Application
    Filed: December 19, 2013
    Publication date: July 31, 2014
    Applicant: Laboratory Corporation of America Holdings
    Inventors: Russell Philip Grant, Stacy Michelle Walker Dee