Xanthan; I.e., Xanthomonas-type Heteropolysaccharides Patents (Class 435/104)
  • Patent number: 9751936
    Abstract: The present invention provides antibodies that bind to serum resistance-associated (SRA) protein of Trypanosoma brucei rhodesiense, and methods of use. According to certain embodiments of the invention, the antibodies are fully human antibodies that bind to SRA. The antibodies of the invention are useful for inhibiting or neutralizing SRA activity, thus providing a means of treating human African trypanosomiasis (sleeping sickness), and symptoms associated with the disease. The antibodies of the invention may also be useful for diagnosis of sleeping sickness.
    Type: Grant
    Filed: February 26, 2014
    Date of Patent: September 5, 2017
    Assignee: REGENERON PHARMACEUTICALS, INC.
    Inventor: Nicholas J. Papadopoulos
  • Patent number: 9725523
    Abstract: The invention relates to mutant strains of the genus Sphingomonas which have a mutation in at least one gene encoding a protein involved in polyhydroxybutyrate (“PHB”) synthesis that allows the mutant strains to produce PHB-deficient Sphingans. The invention is also directed to a process for preparing a clarified Sphingan solution comprising heating aqueous Sphingan solution, in particular PHB-deficient Sphingan solution, to a clarification temperature of about 30° C. to about 70° C., and treating the solution with a clarification agent and enzymes. In addition, the invention is directed to a food or industrial product comprising a PHB-deficient and/or clarified Sphingan. One particular embodiment of the invention is directed to a clarified, PHB-deficient high-acyl gellan and the processes of making thereof.
    Type: Grant
    Filed: March 4, 2016
    Date of Patent: August 8, 2017
    Assignee: CP KELCO U.S., INC.
    Inventors: Stan Bower, Ellen Burke, Nancy E. Harding, Yamini N. Patel, J. Carrie Schneider, Dagmar Meissner, Neil A. Morrison, Ralph Bezanson
  • Patent number: 9572845
    Abstract: The present invention provides Lactobacillus plantarum CJLP56 KCTC 11402BP, a composition containing the Lactobacillus for treating intestinal diseases, and a composition containing the Lactobacillus for enhancing immunity.
    Type: Grant
    Filed: September 1, 2009
    Date of Patent: February 21, 2017
    Assignee: CJ CHEILJEDANG CORP.
    Inventors: Bong-Joon Kim, Heon Woong Jung, Sang-Hyun Seo, Kang-Pyo Lee, Kwang-Woo Hwang, Tae-Joon Won
  • Patent number: 9572846
    Abstract: The present invention provides Lactobacillus plantarum CJLP55 KCTC 11401BP, a composition for treating bowel disease containing the lactobacillus, and a composition for immunological enhancement containing the lactobacillus.
    Type: Grant
    Filed: September 1, 2009
    Date of Patent: February 21, 2017
    Assignee: CJ CHEILJEDANG CORP.
    Inventors: Bong Joon Kim, Heon Woong Jung, Ji Hoon Koh, Tae Jin Kim, Kang-Pyo Lee, Kwang Woo Hwang, Tae Joon Won
  • Patent number: 8921077
    Abstract: The production of a diutan polysaccharide exhibiting increased viscosity properties as compared with previously produced polysaccharide of the same type of repeating units. Such an improved diutan polysaccharide is produced through the generation of a derivative of Sphingomonas sp. ATCC 53159 that harbors a multicopy broad host range plasmid into which genes for biosynthesis of diutan polysaccharide have been cloned. The inventive methods of production of such an improved diutan polysaccharide, as well as the novel cloned genes required to produce the improved diutan within such a method, are also encompassed within this invention. Additionally, the novel engineered Sphingomonas strain including the needed DNA sequence is encompassed within this invention.
    Type: Grant
    Filed: August 30, 2012
    Date of Patent: December 30, 2014
    Assignee: CP Kelco U.S., Inc.
    Inventors: Nancy E. Harding, Yamini N. Patel, Russell Coleman, Steven Matzke
  • Patent number: 8647846
    Abstract: A method and a container for production of a biomembrane are disclosed in the present invention. The biomembrane produced in the method and in the container of the present invention can be processed into a mask, wherein processes for changing the shape of the biomembrane are unnecessary. The mask produced from the biomembrane can be efficient to maintain the skin, and to supply the moisture thereto.
    Type: Grant
    Filed: December 3, 2007
    Date of Patent: February 11, 2014
    Inventor: Connie Hui-Ju Hsu
  • Publication number: 20130288932
    Abstract: According to an embodiment, a method of providing a biopolymer comprises: cryodesiccating a broth, wherein the broth comprises: (A) a biopolymer, wherein the biopolymer is an exopolysaccharide; and (B) a liquid growth medium. According to certain embodiments, the biopolymer is produced by the fermentation of at least one carbohydrate via a microbe. According to an embodiment, the microbe is a bacterium. According to another embodiment, the microbe is a fungus.
    Type: Application
    Filed: April 27, 2012
    Publication date: October 31, 2013
    Applicant: HALLIBURTON ENERGY SERVICES, INC.
    Inventors: Andrew A.F. Mackenzie, Eric Davidson
  • Publication number: 20120021112
    Abstract: Xanthan gums with improved characteristics are provided, a method for preparing the xanthan gums as well as compositions and products comprising the xanthan gums. In particular, the invention relates to xanthan gums obtained from Xanthomonas campestris strains, pathovar cynarae CFBP 19, juglandis CFBP 176, pelargonii CFBP 64, phaseoli CFBP 412 or ATCC 17915, celebenois ATCC 19046, or corylina CFBP 1847 or from a derivative or progeny thereof and to methods of producing xanthan gum from these strains.
    Type: Application
    Filed: March 30, 2010
    Publication date: January 26, 2012
    Applicant: Danisco A/S
    Inventors: Graham Sworn, Emmanuel Kerdavid, Paule Chevallereau, José Fayos
  • Patent number: 8053216
    Abstract: A new method to produce formulations of bacterial cellulose that exhibit improved viscosity-modifying properties particularly with low energy applied to effectuate viscosity changes therewith is provided. Such a method includes the novel co-precipitation with a water soluble co-agent that permits precipitation in the presence of excess alcohol to form an insoluble fiber that can than be utilized as a thickener or suspension aid without the need to introduce high energy mixing. Such bacterial cellulose properties have been available in the past but only through highly labor and energy intensive processes. Such an inventive method as now proposed thus provides a bacterial cellulose-containing formulation that exhibits not only properties that are as effective as those for previous bacterial celluloses, but, in some ways, improvements to such previous types. Certain end-use compositions and applications including these novel bacterial cellulose-containing formulations are also encompassed within this invention.
    Type: Grant
    Filed: May 23, 2005
    Date of Patent: November 8, 2011
    Assignee: CP Kelco U.S., Inc.
    Inventors: Zhi-Fa Yang, Neil A. Morrison, Todd A. Talashek, David F. Brinkmann, Don DiMasi, You Lung Chen
  • Patent number: 8034604
    Abstract: Increasing the molecular length of xanthan polymer makes a higher viscosity xanthan composition. Xanthan with higher specific viscosity characteristics provides more viscosity at equivalent concentration in food, industrial and oilfield applications. Methods for increasing the viscosity of xanthan include inducing particular key genes and increasing copy number of particular key genes.
    Type: Grant
    Filed: December 6, 2010
    Date of Patent: October 11, 2011
    Assignees: C.P. Kelco U.S., Inc., Fundacion Instituto Leloir
    Inventors: Yamini Patel, Jane C. Schneider, Luis Ielpi, Maria Veronica Ielmini
  • Patent number: 7910339
    Abstract: The invention provides a process for producing polysaccharide gum comprising extracting a plant material with an aqueous solvent to produce a polysaccharide gum-containing extract; contacting the extract with at least one proteolytic enzyme to at least partially digest proteins in the extract; adding an organic solvent to the extract to precipitate the polysaccharide gum; and collecting the precipitated polysaccharide gum.
    Type: Grant
    Filed: July 12, 2006
    Date of Patent: March 22, 2011
    Assignee: Her Majesty the Queen in right of Canada as represented by the Minister of Agriculture and Agri-Food Canada
    Inventors: Peter R. Chang, Debbie P. Anderson, Tara C. McIntosh, Neil D. Westcott
  • Patent number: 7868168
    Abstract: Increasing the molecular length of xanthan polymer makes a higher viscosity xanthan composition. Xanthan with higher specific viscosity characteristics provides more viscosity at equivalent concentration in food, industrial and oilfield applications. Methods for increasing the viscosity of xanthan include inducing particular key genes and increasing copy number of particular key genes.
    Type: Grant
    Filed: September 16, 2008
    Date of Patent: January 11, 2011
    Assignees: C.P. Kelco U.S., Inc., Fundacion Instituto Leloir
    Inventors: Yamini Patel, Jane C. Schneider, Luis Ielpi, Maria Veronica Ielmini
  • Patent number: 7727747
    Abstract: The present invention presents a method for the aerobic production of xanthan by bacteria of the genus Xanthomonas on a solid or semi-solid substrate. In the exemplary embodiment of the method, a substrate is provided that has a total solids content of about 6.5% or higher. The substrate is sterilized and cooled. Bacteria of the genus Xanthomonas are inoculated into the substrate and incubated. After incubation, the bacteria are destroyed, the substrate is either diluted or washed, and the xanthan is isolated.
    Type: Grant
    Filed: November 14, 2006
    Date of Patent: June 1, 2010
    Assignee: Bemidji State University Foundation
    Inventors: Dragoljub D. Bilanovic, Samuel Hunter Malloy, Petra Remeta
  • Patent number: 7718405
    Abstract: A fermentation process uses substantially pure oxygen as the sole gas introduced into a fermentation vessel while fermentation reactions are active. The oxygen is introduced at multiple locations along the height of the vessel, and is preferably introduced in the vicinity of the blades of an impeller. The invention enhances the rate at which oxygen can be transferred to the fermentation medium, and is especially useful for fermentation media which become very viscous as the process continues. The invention can be used in the manufacture of viscous products such as xanthan gum, or other products having viscosities greater than about 100 cp.
    Type: Grant
    Filed: September 8, 2006
    Date of Patent: May 18, 2010
    Assignee: American Air Liquide, Inc.
    Inventor: Sudhir Brahmbhatt
  • Publication number: 20090226962
    Abstract: DNA constructs and genetically engineered microbial strains constructed using these DNA constructs, which produce a nuclease enzyme with specificity for DNA and/or RNA, are provided. These strains secrete nuclease into the periplasm or growth medium in an amount effective to enhance productivity and/or recovery of polymer, and are particularly suited for use in high cell density fermentation processes. These constructs are useful for modifying microbial strains to improve production and recovery processes for polymers such as intracellular proteins, such as enzymes, growth factors, and cytokines; for producing polyhydroxyalkanoates; and for producing extracellular polysaccharides, such as xanthan gum, alginates, gellan gum, zooglan, hyaluronic acid and microbial cellulose.
    Type: Application
    Filed: October 25, 2007
    Publication date: September 10, 2009
    Inventors: Gjalt W. Huisman, Laura Z. Luo, Oliver P. Peoples
  • Patent number: 7517675
    Abstract: A method of oxidising carbohydrates and/or carbohydrate derivatives having primary alcohol groups comprising contacting a reaction medium containing said carbohydrates and/or carbohydrate derivatives with a nitroxy radical mediator and a peroxidase enzyme, characterized in that the initial reaction medium contains at least 10% by weight carbohydrates and/or carbohydrate derivatives, in that the peroxidase enzyme is an oilseed peroxidase and in that a hydroperoxide and an alkali compound are gradually added to the reaction medium such that its pH is maintained at between 3.5 and 10.0 (pH-Stat); use of said method in a process for producing gluconic acid, glucaric acid and/or D-glucuronolactone.
    Type: Grant
    Filed: December 17, 2004
    Date of Patent: April 14, 2009
    Assignee: Cerestar Holding B.V.
    Inventors: Ronny Leontina Marcel Vercauteren, An Amanda Jules Heylen
  • Publication number: 20090093626
    Abstract: Increasing the molecular length of xanthan polymer makes a higher viscosity xanthan composition. Xanthan with higher specific viscosity characteristics provides more viscosity at equivalent concentration in food, industrial and oilfield applications. Methods for increasing the viscosity of xanthan include inducing particular key genes and increasing copy number of particular key genes.
    Type: Application
    Filed: September 16, 2008
    Publication date: April 9, 2009
    Inventors: Yamini Patel, Jane C. Schneider, Luis Ielpi, Maria Veronica Ielmini
  • Publication number: 20080286839
    Abstract: A method and a container for production of a biomembrane are disclosed in the present invention. The biomembrane produced in the method and in the container of the present invention can be processed into a mask, wherein processes for changing the shape of the biomembrane are unnecessary. The mask produced from the biomembrane can be efficient to maintain the skin, and to supply the moisture thereto.
    Type: Application
    Filed: December 3, 2007
    Publication date: November 20, 2008
    Inventor: Connie Hui-Ju Hsu
  • Patent number: 7439044
    Abstract: Increasing the molecular length of xanthan polymer makes a higher viscosity xanthan composition. Xanthan with higher specific viscosity characteristics provides more viscosity at equivalent concentration in food, industrial and oilfield applications. Methods for increasing the viscosity of xanthan include inducing particular key genes and increasing copy number of particular key genes.
    Type: Grant
    Filed: March 17, 2004
    Date of Patent: October 21, 2008
    Assignee: CP Kelco U.S., Inc.
    Inventors: Yamini Patel, Jane C. Schneider, Luis Ielpi, Maria Veronica Ielmini
  • Patent number: 7376117
    Abstract: Interleaving circuit for a multiband OFDM (orthogonal frequency division multiplexing) transceiver of a ultra wide band wireless personal access network transmitting OFDM modulated symbols, wherein each OFDM symbol consists of a predetermined number (NCBPS) of encoded bits, said interleaving circuit comprising a symbol interleaving unit which receives an input bitstream of encoded bits and permutes adjacent bits of said input bitstream across different OFDM symbols; an intra-symbol tone interleaving unit which receives the bits permuted by said symbol interleaving unit and permutes adjacent bits of each OFDM symbol across uncorrelated data sub-carriers; and an intra-symbol cyclic shift unit which shifts cyclically NCBPS bits of each OFDM symbol in response to a shift value (K) which is changed between adjacent OFDM symbols.
    Type: Grant
    Filed: November 12, 2004
    Date of Patent: May 20, 2008
    Assignee: Infineon Technologies AG
    Inventors: Yossi Erlich, Assaf Gurevitz
  • Publication number: 20080113414
    Abstract: The present invention presents a method for the aerobic production of xanthan by bacteria of the genus Xanthomonas on a solid or semi-solid substrate. In the exemplary embodiment of the method, a substrate is provided that has a total solids content of about 6.5% or higher. The substrate is sterilized and cooled. Bacteria of the genus Xanthomonas are inoculated into the substrate and incubated. After incubation, the bacteria are destroyed, the substrate is either diluted or washed, and the xanthan is isolated.
    Type: Application
    Filed: November 14, 2006
    Publication date: May 15, 2008
    Applicants: Bemidji State University Foundation, State of Minnesota Environment and Natural Resources Trust Fund
    Inventors: Dragoljub D. Bilanovic, Samuel Hunter Malloy, Petra Remeta
  • Patent number: 7015206
    Abstract: The invention concerns the use of a polysaccharide excreted by the Vibrio diabolicus species for making a medicine with cicatrising activity, in particular bone repair activity. The invention also concerns a bone reconstruction biomaterial comprising said polysaccharide and a bone endoprosthesis whereof the surface is coated with such a polysaccharide.
    Type: Grant
    Filed: July 4, 2001
    Date of Patent: March 21, 2006
    Assignee: Institut Francais de le Recherche pour l'Exploitation de la Mer
    Inventors: Jean Guezennec, Philippe Zanchetta
  • Patent number: 6933137
    Abstract: Animal-free meninge fermentation media and process is developed based upon use of a chemically defined medium. To improve polysaccharide production, fed-batch fermentation is examined using different feed solutions and feeding strategies. A feed solution containing glucose, amino acids, and trace metal elements produces Group A polysaccharide at approximately 3 times the level observed with batch fermentation. This process is used successfully to produce polysaccharides of N. meningitidis serotypes, A, C, Y and W-135 and is run reproducibly at the 20 L scale and can be scaled to 400 L or more.
    Type: Grant
    Filed: May 16, 2003
    Date of Patent: August 23, 2005
    Assignee: Aventis Pasteur
    Inventors: Richard C. Egen, Lori Ann Fortin, Willie Wei Qiang Sun
  • Patent number: 6855524
    Abstract: The invention concerns a method for producing exopolysaccharides by fermenting micro-organisms characterised in that it consists in carrying out the fermentation in a nutrient medium comprising at least a source of carbon available to the micro-organisms and at least a source of nitrogen, said source being derived from a fraction of carob seed.
    Type: Grant
    Filed: September 6, 2000
    Date of Patent: February 15, 2005
    Assignee: Danisco USA Inc.
    Inventors: Olivier Nore, Jean-Luc Simon
  • Publication number: 20040259839
    Abstract: Increasing the molecular length of xanthan polymer makes a higher viscosity xanthan composition. Xanthan with higher specific viscosity characteristics provides more viscosity at equivalent concentration in food, industrial and oilfield applications. Methods for increasing the viscosity of xanthan include inducing particular key genes and increasing copy number of particular key genes.
    Type: Application
    Filed: March 17, 2004
    Publication date: December 23, 2004
    Inventors: Yamini Patel, Jane C. Schneider, Luis Ielpi, Maria Veronica Ielmini
  • Patent number: 6586213
    Abstract: A process is disclosed for clarifying an aqueous xanthan gum solution comprising treatment of the xanthan solution with at least one chelating agent, surfactant, organic acid, or a mixture thereof, and with a protease enzyme or a lysozyme and a protease enzyme. Also disclosed is a highly purified solid xanthan gum and compositions containing the same, wherein the gum is obtained from the clarified xanthan gum solution.
    Type: Grant
    Filed: April 13, 2001
    Date of Patent: July 1, 2003
    Assignee: CP Kelco U.S., Inc.
    Inventors: Joseph M. Kobzeff, Daniel E. Mead, Todd A. Talashek, Don DiMasi
  • Publication number: 20030087766
    Abstract: The present disclosure relates to a lubricating composition usable for lubricating an interface between a tire and a safety support mounted on a wheel rim within said tire, and a mounted assembly for an automobile provided with said lubricating composition. The lubricating composition according to the invention comprises an aqueous or non aqueous lubricating agent, and a polysaccharide intended to thicken said lubricating agent. The lubricating agent comprises glycerine in a mass fraction equal to or greater than about 60% of the lubricating agent and the lubricating agent is present in said composition in a mass fraction of between about 95% and 99%. The invention also relates to the lubrication between the support and the tire when traveling on a flat tire, following a drop in inflation pressure within said tire.
    Type: Application
    Filed: March 6, 2002
    Publication date: May 8, 2003
    Inventors: Georges Salaun, Jean-Louis Mauclin
  • Publication number: 20030036176
    Abstract: DNA and protein sequences from Xanthomonas campestris pv. campestris, which may be targeted for activity reduction or enhancement using directed genetic engineering. Transformed microorganisms having reduced activity of at least one protein, e.g. galactomannanase, can be provided by disrupting a gene encoding the protein or introducing antisense nucleic acid sequence, providing xanthan gum essentially free of galactomannanase, amylase, cellulase or protease activity.
    Type: Application
    Filed: August 10, 2001
    Publication date: February 20, 2003
    Inventors: Stanley G. Bower, Thomas M. Ramseier
  • Patent number: 6509176
    Abstract: The invention concerns a heteropolysaccharide (HP) characterised in that it is obtainable by fermenting in a medium comprising at least an Agrobacterium radiobacter I-2001 (or DSM 12095) strain, one of its recombinants, or one of its mutants, and a carbon source capable of being assimilated by said strain, one of its recombinants or one of its mutants. The invention also concerns a method for preparing said heteropolysaccharide and its use as thickening and/or gelling agent.
    Type: Grant
    Filed: June 25, 2001
    Date of Patent: January 21, 2003
    Assignee: Rhodia Chimie
    Inventors: Robert Cantiani, Alain Senechal, Sophie Vaslin, Paule Chevallereau, Jean-Luc Simon
  • Patent number: 6464384
    Abstract: A system for providing improved bulk liquid mixing and effective gas-liquid contacting for mass transfer of the gas to the liquid, especially a non-Newtonian liquid, the viscosity of which decreases when under shearing conditions (shear thinning), in an upright tank. An upright draft tube is mounted within the tank and has a lower end spaced from the tank bottom and an upper end spaced below the surface of the liquid in the tank. A plurality of mixing impellers in the draft tube are sufficiently close to each other to establish a field or pattern of agitation to cause shear thinning and upflow throughout the draft tube and which produces significant turbulence at the liquid surface. A plurality of radially inwardly projecting, circumferentially spaced baffles extend from the draft tube and are proximate the mixing impellers to prevent swirling of the liquid within the draft tube.
    Type: Grant
    Filed: July 2, 2001
    Date of Patent: October 15, 2002
    Assignee: The Penn State Research Foundation
    Inventors: Paul M. Kubera, John R. McWhirter, Bradley S. Dominik, Prakash G. Balan
  • Patent number: 6410265
    Abstract: This invention relates to the isolation of a novel putative efflux gene from Pseudomonas mendocina. The putative efflux gene is useful for probing an organism's efflux system to gain an understanding of the mechanisms of solvent tolerance. The invention further provides a Pseudomonas mendocina strain deficient in this gene. This strain is unable to grow in the presence of chloramphenicol and, compared to the wildtype strain, grows slowly in the presence of high concentrations of PHBA.
    Type: Grant
    Filed: October 30, 1998
    Date of Patent: June 25, 2002
    Assignee: E. I. du Pont de Nemours and Company
    Inventor: Kevin Keqin Chen
  • Patent number: 6399338
    Abstract: The invention relates to a method of producing chitin or chitosan by culturing a Rhizopus azygosporus fungus or an Actinomucor taiwanensis fungus and isolating chitosan or chitin from the culture.
    Type: Grant
    Filed: September 27, 2000
    Date of Patent: June 4, 2002
    Assignee: Food Industry Research and Development Institute
    Inventors: Mei-Huei Chen, Hing-Yuen Chan, Chih-Lu Wu, Su-Hui Chuang, Ing-Er Hwang, Yen-Lin Chen, Gwo-Fang Yuan
  • Patent number: 6391596
    Abstract: The present invention is related a high viscosity xanthan and processes for producing said high viscosity xanthan. In particular, the invention relates to a process for increasing the viscosity of xanthan comprising the steps of: (i) diluting xanthan in a fermentation broth with more than 30 percent by volume of a diluent to said fermentation broth volume; and (ii) pasteurizing said diluted xanthan fermentation broth. A particularly preferred pasteurization process is to utilize steam from a direct steam injection system. Xanthans with increased viscosities have improved product functionality for a number of different commercial applications are produced using the processes of the present invention.
    Type: Grant
    Filed: September 19, 2000
    Date of Patent: May 21, 2002
    Assignee: CP Kelco U.S., Inc.
    Inventors: Todd A. Talashek, William Scott Bousman
  • Publication number: 20010055237
    Abstract: A system for providing improved bulk liquid mixing and effective gas-liquid contacting for mass transfer of the gas to the liquid, especially a non-Newtonian liquid, the viscosity of which decreases when under shearing conditions (shear thinning), in an upright tank. A process, such as fermentation which produces commercial quantities of polysaccharides such as xanthan gum. may be carried out in the tank. An upright draft tube is mounted within the tank and has a lower end spaced from the tank bottom and an upper end spaced below the surface of the liquid in the tank. A plurality of mixing impellers in the draft tube are sufficiently close to each other to establish a field or pattern of agitation to cause shear thinning and upflow throughout the draft tube and which may produce turbulence at the liquid surface. A plurality of radially inwardly projecting. circumferentially spaced baffles extend from the draft tube and are proximate the mixing impellers to prevent swirling of the liquid within the draft tube.
    Type: Application
    Filed: July 2, 2001
    Publication date: December 27, 2001
    Inventors: Paul M. Kubera, John R. McWhirter, Bradley S. Dominik, Prakash G. Balan
  • Patent number: 6316614
    Abstract: Variant xanthan gums are provided which include a water-soluble polysaccharide polymer comprising repeating pentamer units having a D-glucose:D-mannose:D-glucuronic acid ratio of about 2:2:1, and a water-soluble polysaccharide polymer comprising repeating tetramer units having a D-glucose:D-mannose:D-glucuronic acid ratio of about 2:1:1. The D-glucose moieties are linked in a beta-[1,4] configuration. The inner D-mannose moieties are linked in an alpha-[1,3] configuration, generally to alternate glucose moieties. The D-glucuronic acid moieties are linked in a beta-[1,2] configuration to the inner mannose moieties. The outer mannose moieties are linked to the glucuronic acid moieties in a beta-[1,4] configuration. Processes for preparing the polysaccharide polymers are also provided.
    Type: Grant
    Filed: June 7, 1995
    Date of Patent: November 13, 2001
    Assignee: CP Kelco U.S., Inc.
    Inventors: Daniel H. Doherty, Donna M. Ferber, John D. Marrelli, Rebecca W. Vanderslice, Randal A. Hassler
  • Patent number: 6251641
    Abstract: A treatment for enhancing the recovery of exopolysaccharide from bacterial cells is disclosed, which treatment includes adding to a cultivation medium containing said cells an amount, effective for the purpose of an alkylsulfosuccinate surfactant.
    Type: Grant
    Filed: December 21, 1999
    Date of Patent: June 26, 2001
    Assignee: BetzDearborn Inc.
    Inventor: J. Barry Wright
  • Patent number: 6251446
    Abstract: A food or pharmaceutical composition including a liquid composition comprising the liquid fermentation broth product of a biologically active substance, in a carbohydrate medium other than dairy whey wherein said liquid fermentation broth product has not been subject to any concentrating or drying steps prior to introduction of the broth product into said food or pharmaceutical composition is provided.
    Type: Grant
    Filed: April 17, 2000
    Date of Patent: June 26, 2001
    Assignee: Rhodia Inc.
    Inventors: Craig Alan Hoppe, Jeanette Lawrence, Amr Shaheed
  • Patent number: 6110875
    Abstract: Xanthan molecules are degraded using an xanthanase enzyme complex that is stable at temperatures above 250.degree. F., such as those temperatures found in some wellbores and process streams. The xanthanase enzyme complex is produced by a novel soil bacterium. The xanthanase enzyme complex may be used to remove xanthan based formation damage, such as drilling filter-cakes and filtrates, or to remove xanthan based filter-cakes and/or residues present in processing equipment. The xanthanase enzyme complex may also be used to reduce the viscosity of xanthan-containing fluids, such as hydraulic fracturing fluids, blocking gels, drilling muds, and process fluids. The xanthanase enzyme complex may also be used in conjunction with other well or process treatments, such as stimulations and cementing operations, to improve the effectiveness of these treatments.
    Type: Grant
    Filed: March 7, 1997
    Date of Patent: August 29, 2000
    Assignee: BJ Services Company
    Inventors: Robert M. Tjon-Joe-Pin, Michelle Alana Carr, Bing Yang
  • Patent number: 6066479
    Abstract: A treatment for enhancing the recovery of exopolysaccharide from bacterial cells is disclosed, which treatment includes adding to a cultivation medium containing said cells an amount, effective for the purpose of an alkylsulfosuccinate surfactant.
    Type: Grant
    Filed: August 13, 1998
    Date of Patent: May 23, 2000
    Assignee: Betzdearborn Inc.
    Inventor: J. Barry Wright
  • Patent number: 6030817
    Abstract: A new recombinant bacteria for the production of exopolysaccharides is disclosed as well as a method for making the recombinant bacteria and making an exopolysaccharide from the bacteria by submerged aerobic fermentation of the bacteria utilizing a sugar substrate. The exopolysaccharides obtained from the inventive bacteria exhibit improved, more desirable or different properties from the exopolysaccharide produced by the non-recombinant bacteria from which the recombinant bacteria was derived. In addition, the present invention provides a method of producing bacterial exopolysaccharides by fermentation from sugar substrates that the bacteria which the exopolysaccharides are native to cannot utilize.
    Type: Grant
    Filed: June 11, 1998
    Date of Patent: February 29, 2000
    Assignees: Shin-Etsu Bio, Inc., Shin-Etsu Chemical Co., Ltd.
    Inventors: Thomas J. Pollock, Marcia Mikolajczak, Motohide Yamazaki, Linda Thorne, Richard W. Armentrout
  • Patent number: 6027925
    Abstract: A recombinant bacteria for the production of exopolysaccharides is disclosed as well as a method for making the recombinant bacteria and making an exopolysaccharide from the bacteria by submerged aerobic fermentation of the bacteria utilizing a sugar substrate. In addition, the present invention provides a method of producing bacterial exopolysaccharides by fermentation from sugar substrates that the wild-type bacteria for producing the exopolysaccharide cannot utilize.
    Type: Grant
    Filed: June 12, 1998
    Date of Patent: February 22, 2000
    Assignees: Shin-Etsu Bio, Inc., Shin-Etsu Chemical Co., Ltd.
    Inventors: Thomas J. Pollock, Marcia Mikolajczak, Motohide Yamazaki, Linda Thorne, Richard W. Armentrout
  • Patent number: 5994107
    Abstract: Xanthan gum is purified by heat-treating a xanthan gum fermented broth, and consecutively treating the broth first with alkaline protease and then with lysozyme or in reverse order, and thereafter recovering xanthan gum from the treated broth. A clear aqueous solution of xanthan gum may be obtained without complex procedures. The xanthan gum is separated and purified and 0.3% aqueous solution of the purified xanthan gum has a transmittance of at least 80%.
    Type: Grant
    Filed: December 31, 1997
    Date of Patent: November 30, 1999
    Assignees: Shin-Etsu Chemical Co., Ltd., Shin-Etsu Bio, Inc.
    Inventors: Kanji Murofushi, Taira Homma, Shigehiro Nagura, Richard Armentrout
  • Patent number: 5985623
    Abstract: The present invention relates to DNA segments isolated from Sphingomonas sp. and involved in the biosynthetic production of sphingan polysaccharides to increase the production of the polysaccharide in engineered microorganisms. The present invention also relates to methods of engineering strains of Sphingomonas to produce bacteria which are hyperproducers of sphingan, methods of identifying and utilizing DNA fragments useful to enhance production of sphingan in bacteria and the hyperproducer bacteria.
    Type: Grant
    Filed: January 24, 1995
    Date of Patent: November 16, 1999
    Assignees: Shin-Etsu Bio, Inc., Shin-Etsu Chemical Co., Ltd.
    Inventors: Thomas J. Pollock, Motohide Yamazaki, Linda Thorne, Marcia Mikolajczak, Richard W. Armentrout
  • Patent number: 5972661
    Abstract: A system for providing improved bulk liquid mixing and effective gas-liquid contacting for mass transfer of the gas to the liquid, especially a non-Newtonian liquid, the viscosity of which decreases when under shearing conditions (shear thinning), in an upright tank. A process, such as fermentation which produces commercial quantities of polysaccharides such as xanthan gum, may be carried out in the tank. An upright draft tube is mounted within the tank and has a lower end spaced from the tank bottom and an upper end spaced below the surface of the liquid in the tank. A plurality of mixing impellers in the draft tube are sufficiently close to each other to establish a field or pattern of agitation to cause shear thinning and upflow throughout the draft tube and which may produce turbulence at the liquid surface. A plurality of radially inwardly projecting, circumferentially spaced baffles extend from the draft tube and are proximate the mixing impellers to prevent swirling of the liquid within the draft tube.
    Type: Grant
    Filed: September 28, 1998
    Date of Patent: October 26, 1999
    Assignees: Penn State Research Foundation, General Signal Corporation
    Inventors: Paul M. Kubera, John R. McWhirter, Bradley S. Dominik, Prakash G. Balan
  • Patent number: 5972695
    Abstract: A fermenter for the production of xanthan gum is provided with an upper helical impeller and a lower turbine impeller which are positioned therewithin, and an agitator shaft for driving these helical impeller and turbine impeller. The helical impeller consists of a pair of vertically spaced arms extending from the agitator shaft in opposite directions and arranged in twisted relationship, and at least one shearing paddle bridging these arms, and the turbine impeller consists of a rotating disc having at least one turbine blade attached thereto.
    Type: Grant
    Filed: October 14, 1997
    Date of Patent: October 26, 1999
    Assignees: Shin-Etsu Chemical Co., Ltd., Shin-Etsu Bio, Inc
    Inventors: Kanji Murofushi, Shigehiro Nagura, Taira Homma
  • Patent number: 5948651
    Abstract: Water-soluble polysaccharide polymers are provided including a polymer composed of repeating pentamer units having a D-glucose:D-mannose:D-glucuronic acid ratio of about 2:2:1, and a polymer composed of repeating tetramer units having a D-glucose:D-mannose:D-glucuronic acid ratio of about 2:1:1. The D-glucose moieties are linked in a beta-?1,4! configuration. The inner D-mannose moieties are linked in an alpha-?1,3! configuration, generally to alternate glucose moieties. The D-glucuronic acid moieties are linked in a beta-?1,2! configuration to the inner mannose moieties. The outer mannose moieties are linked to the glucuronic acid moieties in a beta-?1,4! configuration. Also an isolated acetylase deficient mutant of Xanthomonas is used in a process to produce the polysaccharide.
    Type: Grant
    Filed: March 20, 1995
    Date of Patent: September 7, 1999
    Assignee: Monsanto Company
    Inventors: Daniel H. Doherty, Randal A. Hassler
  • Patent number: 5912151
    Abstract: A method of increasing xanthan gum production, comprising culturing a Xanthomonas campestris strain having a xanthan-increasing modification in a culture medium, wherein the modification is selected from the group consisting of (1) a mutation causing rifampicin-resistance; (2) a mutation causing bacitracin-resistance; or (3) exogenous genetic information controlling the synthesis of xanthan; and separating xanthan from the culture medium, is provided along with specific DNA sequences and Xanthomonas campestris strains showing increased xanthan gum production.
    Type: Grant
    Filed: January 24, 1992
    Date of Patent: June 15, 1999
    Assignees: Shin-Etsu Chemical Co., Ltd., Shin-Etsu Bio, Inc.
    Inventors: Thomas J. Pollock, Linda Thorne
  • Patent number: 5854034
    Abstract: The present invention relates to DNA segments isolated from Sphingomonas sp. and involved in the biosynthetic production of sphingan polysaccharides to increase the production of the polysaccharide in engineered microorganisms. The present invention also relates to methods of engineering strains of Sphingomonas to produce bacteria which are hyperproducers of sphingan, methods of identifying and utilizing DNA fragments useful to enhance production of sphingan in bacteria and the hyperproducer bacteria.
    Type: Grant
    Filed: January 24, 1996
    Date of Patent: December 29, 1998
    Assignees: Shin-Etsu Cio, Inc., Shin-Etsu Chemical Co., Ltd.
    Inventors: Thomas J. Pollock, Motohide Yamazaki, Linda Thorne, Marcia Mikolajczak, Richard W. Armentrout
  • Patent number: 5834043
    Abstract: New Lactobacillus sake like strains are provided obtainable from meat products, which strains are capable of producing an exopolysaccharide having shear-thinning properties, even at low concentrations, and/or thickening and/or emulsion-stabilizing properties.
    Type: Grant
    Filed: September 19, 1995
    Date of Patent: November 10, 1998
    Assignee: Quest International B.V.
    Inventors: Dirk Johannes Cornelis Van Den Berg, Adrianus Marinus Ledeboer, Gerard Willem Robijn, Robert Vreeker
  • Patent number: 5756317
    Abstract: Heteropolysaccharide biopolymers well adopted as thickening agents are improvedly produced by microbially fermenting a carbohydrate nutrient medium, said nutrient medium comprising an oil-in-water emulsion of a discontinuous oily phase dispersed within a continuous aqueous phase.
    Type: Grant
    Filed: January 16, 1991
    Date of Patent: May 26, 1998
    Assignee: Rhone-Poulenc Specialities Chimiques
    Inventors: Veronique Leproux, Michel Peignier, Patrick Cros, Jeanine Beucherie, Yves Kennel