Tryptophan; Tyrosine; Phenylalanine; 3,4 Dihydroxyphenylalanine Patents (Class 435/108)
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Publication number: 20030167514Abstract: The present invention provides a method for conferring tolerance to an amino acid analog of tryptophan to a plant and/or altering the tryptophan content of a plant by introducing and expressing an isolated DNA segment encoding an anthranilate synthase in the cells of the plant. Transgenic plants transformed with an isolated DNA segment encoding an anthranilate synthase, as well as seeds and progeny derived from these plants, are also provided. The present invention also provides a cDNA sequence of an alpha and a beta subunit of a maize anthranilate synthase.Type: ApplicationFiled: January 29, 2003Publication date: September 4, 2003Inventors: Paul C. Anderson, Paul S. Chomet, Matthew C. Griffor, Alan L. Kriz
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Publication number: 20030157667Abstract: A method for producing L-amino acid, such as L-phenylalanine and L-tryptophan, is provided using bacterium belonging to the genus Escherichia wherein the L-amino acid productivity of said bacterium is enhanced by enhancing an activity of protein encoded by the yddG gene.Type: ApplicationFiled: November 25, 2002Publication date: August 21, 2003Applicant: Ajinomoto Co., Inc.Inventors: Maria Viacheslavovna Vitushkina, Vitaliy Arkadyevich Livshits, Sergei Vladimirovich Mashko, Vera Georgievna Doroshenko, Irina Vladimirovna Biryukova, Zhanna Iosifovna Katashkina, Aleksandra Yurievna Skorokhodova, Alla Valentinovna Belareva
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Publication number: 20030148473Abstract: A method for producing an L-amino acid, such as L-phenylalanine, L-threonine is provided using bacterium belonging to the genus Escherichia wherein the L-amino acid productivity of said bacterium is enhanced by enhancing an activity of protein encoded by the yedA gene.Type: ApplicationFiled: November 25, 2002Publication date: August 7, 2003Applicant: Ajinomoto Co., Inc.Inventors: Vitaliy Arkadyevich Livshits, Maria Viacheslavovna Vitushkina, Mikhail Markovich Gusyatiner, Mikhail Kharisovich Ziyatdinov, Valery Zavenovich Akhverdian, Ekaterina Alekseevna Savrasova, Vera Georgievna Doroshenko, Sergey Vladimirovich Mashko
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Publication number: 20030138918Abstract: A bacterium belonging to the genus Escherichia having an ability to produce an L-amino acid is cultured in a medium containing fructose as a main carbon source, preferably a medium containing a carbon source composed of 30 weight % or more of fructose and 70 weight % or less of glucose, to produce and accumulate the L-amino acid in the medium, and the L-amino acid is collected from the medium.Type: ApplicationFiled: December 20, 2002Publication date: July 24, 2003Applicant: AJINOMOTO CO., INC.Inventors: Shintaro Iwatani, Masaki Kobayashi
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Publication number: 20030129711Abstract: This invention relates to an isolated nucleic acid fragment encoding a tryptophan biosynthetic enzyme. The invention also relates to the construction of a chimeric gene encoding all or a portion of the tryptophan biosynthetic enzyme, in sense or antisense orientation, wherein expression of the chimeric gene results in production of altered levels of the tryptophan biosynthetic enzyme in a transformed host cell.Type: ApplicationFiled: November 15, 2002Publication date: July 10, 2003Inventors: Rebecca E. Cahoon, Steven Gutteridge, Steven J. Vollmer
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Publication number: 20030096379Abstract: The invention relates to a coupled enzymatic process for producing tryptamine derivatives from indole compounds. In the first enzyme-catalyzed reaction, indole derivatives are converted to tryptophan derivative intermediates, then the tryptophan intermediates are decarboxylated in a second enzymatic reaction in the same reaction system. In this way, tryptamine derivative products are formed from indole derivatives in a single process. The invention is also directed to novel tryptophan and tryptamine derivatives, which can be prepared by the inventive method.Type: ApplicationFiled: March 28, 2002Publication date: May 22, 2003Inventors: James L. Kilgore, J. David Rozzell
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Publication number: 20030097677Abstract: The present invention provides a method for conferring tolerance to an amino acid analog of tryptophan to a plant and/or altering the tryptophan content of a plant by introducing and expressing an isolated DNA segment encoding an anthranilate synthase in the cells of the plant. Transgenic plants transformed with an isolated DNA segment encoding an anthranilate synthase, as well as human or animal food, seeds and progeny derived from these plants, are also provided.Type: ApplicationFiled: May 3, 2002Publication date: May 22, 2003Inventors: Lisa M. Weaver, Jihong Liang, Ridong Chen, Soon Seog Jeong, Timothy Mitsky, Steven Slater, William Rapp
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Patent number: 6559280Abstract: The invention relates to novel compounds comprising a ubiquitination recognition element and a protein binding element. The invention also relates to the use of said compounds for modulating the level and/or activity of a target protein. The compounds are useful for the treatment of disease such as infections, inflammatory conditions, cancer and genetic diseases. The compounds are also useful as insecticides and herbicides.Type: GrantFiled: June 14, 2001Date of Patent: May 6, 2003Assignee: Proteinix, Inc.Inventors: John H. Kenten, Steven F. Roberts
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Publication number: 20030077764Abstract: In a method for producing a target substance by utilizing a microorganism, which comprises culturing the microorganism in a medium to produce and accumulate the target substance in the medium and collecting the target substance from the culture, there is used, as the microorganism, a mutant strain or recombinant strain of a microorganism in which maltose assimilation is controlled by an interaction between IIAGlc protein of glucose PTS and a protein involved in non-PTS uptake of maltose, and the interaction between the IIAGlc protein and the protein involved in non-PTS uptake of maltose is reduced or eliminated in the mutant strain or recombinant strain.Type: ApplicationFiled: April 15, 2002Publication date: April 24, 2003Applicant: AJINOMOTO CO., INCInventors: Nobuharu Tsujimoto, Tomoko Suzuki, Hisao Ito
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Publication number: 20030079255Abstract: Genes encoding phenylalanine ammonia-lyase (PAL), tyrosine ammonia lyase (TAL) and phenylalanine hydroxylase (PAH) have been introduced into a host organism for the production of Para-hydroxycinnamic acid (PHCA). The introduction of these genes results in the redirection of carbon flow in the host, optimizing the flow of carbon from glucose to PHCA. The intermediates, tyrosine and cinnamic acid are also produced.Type: ApplicationFiled: May 3, 2002Publication date: April 24, 2003Inventors: Wei Wei Qi, Fateme Sima Sariaslani, Xiao-Song Tang
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Patent number: 6551803Abstract: A process is described for purifying an amino acid-containing solution by means of electrodialysis, wherein an amino acid-containing solution is employed which is obtained from the fermentation for producing at least one amino acid.Type: GrantFiled: May 1, 2002Date of Patent: April 22, 2003Assignee: BASF AktiengesellschaftInventors: Andreas Fischer, Christoph Martin, Jürgen Müller
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Patent number: 6551816Abstract: The invention concerns a method for preparing &agr;-phenylalanine ester with substantially enantiomeric purity of formulae (I) and (II) wherein Alk represents a linear or branched C1-C6 alkyl radical, characterized in that it consists in contacting a mixture of &agr;-phenylalanine ester enantiomers with a lipase, a protease or an esterase.Type: GrantFiled: December 15, 2000Date of Patent: April 22, 2003Assignee: Rhone-Poulenc AgroInventors: Marie-Claude Bontoux, Olivier Favre-Bulle
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Patent number: 6515201Abstract: The present invention provides a method for conferring tolerance to an amino acid analog of tryptophan to a plant and/or altering the tryptophan content of a plant by introducing and expressing an isolated DNA segment encoding an anthranilate synthase in the cells of the plant. Transgenic plants transformed with an isolated DNA segment encoding an anthranilate synthase, as well as seeds and progeny derived from these plants, are also provided. The present invention also provides a cDNA sequence of an alpha and a beta subunit of a maize anthranilate synthase.Type: GrantFiled: December 8, 2000Date of Patent: February 4, 2003Assignee: DeKalb Genetics CorporationInventors: Paul C. Anderson, Paul S. Chomet, Matthew C. Griffor, Alan L. Kriz
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Patent number: 6489100Abstract: Genetic elements comprising expression vectors and a gene coding for phosphoenol pyruvate synthase is utilized to enhance diversion of carbon resources into the common aromatic pathway and pathways branching therefrom. The overexpression of phosphoenol pyruvate synthase increases DAHP production to near theoretical yields.Type: GrantFiled: November 15, 1999Date of Patent: December 3, 2002Assignee: Texas A & M University SystemInventor: James C. Liao
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Publication number: 20020173011Abstract: A bacterium belonging to the genus Escherichia having an ability to produce an L-amino acid is cultured in a medium containing fructose as a main carbon source, preferably a medium containing a carbon source composed of 30 weight % or more of fructose and 70 weight % or less of glucose, to produce and accumulate the L-amino acid in the medium, and the L-amino acid is collected from the medium.Type: ApplicationFiled: January 18, 2002Publication date: November 21, 2002Applicant: AJINOMOTO CO. INCInventors: Shintaro Iwatani, Masaki Kobayashi
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Publication number: 20020155521Abstract: This invention describes methods for enhancing carbon flow into a pathway of a host cell to enhance the biosynthetic production of compounds therefrom, the host cells being selected based on being phenotypically Pts−/glucose+. Such host cells are capable of transporting glucose without consuming PEP, resulting in conservation of PEP which can be re-directed into the pathway in order to enhance the production of desired compounds along the pathway. Pts−/glucose+ mutants have been shown to be advantageous for the enhanced production of the aromatic amino acids.Type: ApplicationFiled: September 30, 1997Publication date: October 24, 2002Inventors: FERNANDO VALLE, NOEMI MEJIA, ALAN BERRY
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Patent number: 6444450Abstract: A process for large-scale, low cost, batch or continuous production of polyphenols using enzyme-mediated reactions and methods for recycling non-consumed reactants.Type: GrantFiled: January 28, 1998Date of Patent: September 3, 2002Assignee: The United States of America as represented by the Secretary of the ArmyInventors: Joseph A. Akkara, Madhu S. R. Ayyagari, David L. Kaplan
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Publication number: 20020081671Abstract: The invention relates to an enzymatic method for reductive amination of &agr;-ketodicarboxylic acid derivatives or salts thereof using an amino acid dehydrogenase.Type: ApplicationFiled: November 5, 2001Publication date: June 27, 2002Applicant: Degussa AGInventors: Kai Rossen, Martin Sarich, Milan Latinovic, Claudia Rollmann, Andreas Bommarius
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Patent number: 6387687Abstract: A microorganism having indolmycin-producing ability and tryptophan analogue resistance is provided, and indolmycin or its salt can be produced efficiently by cultivating such microorganism in a medium to produce and accumulate indolmycin or its salt in the culture broth followed by recovering a product.Type: GrantFiled: January 23, 2001Date of Patent: May 14, 2002Assignee: Takeda Chemical Industries, Ltd.Inventors: Akihiko Naotsuka, Motoo Izawa, Ken-ichiro Miyagawa
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Patent number: 6365380Abstract: A process for stereoselectively inverting a chiral center of a chemical compound is disclosed. The process first consists of forming a mixture of the chemical comopund, an enzymatic system, and a metal catalyst. Next, the process stereoselectively dehydrogenates a group attached to the chiral center with the enzymatic system in the presence of an oxidant to produce a dehydrogenated group, Lastly, the process hydrogenates the dehydrogenated group with the metal catalyst in the presence of a hydrogen source to stereoselectively invert the chiral center of the chemical compound.Type: GrantFiled: January 22, 2001Date of Patent: April 2, 2002Assignee: PCBU Services, Inc.Inventors: Weiguo Liu, Scott Laneman, David John Ager, Paul Phillip Taylor
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Patent number: 6358714Abstract: The present invention provides materials and methods for production of D-phenylalanine by recombinant expression in a microorganisms of a D-aminotransferase gene and optionally a dadX gene in optional combination with eliminating or reducing expression of L-transaminase genes.Type: GrantFiled: April 19, 1995Date of Patent: March 19, 2002Assignee: The Nutrasweet CompanyInventors: Ian Fotheringham, Paul P. Taylor, Roberta K. Yoshida
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Patent number: 6350596Abstract: A method for producing L-phenylalanine, comprising the steps of: culturing a bacterium belonging to the genus Methylophilus which has an ability to produce L-phenylalanine and is resistant to a phenylalanine analog, in a culture medium to produce and accumulate L-phenylalanine in a culture, and recovering L-phenylalanine from the culture.Type: GrantFiled: February 26, 2001Date of Patent: February 26, 2002Assignee: Ajinomoto Co., Inc.Inventors: Yurgis Antanas Vladovich Iomantas, Elena Georgievna Abalakina
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Publication number: 20020012975Abstract: A process for producing &agr;-amino acid from starting material comprising &agr;-amino amide enantiomers (A) and (B) is such that enantiomer (A) is converted preferentially over enantiomer (B) so that a time independent excess of at least 90%, preferably at least 98% is given. The reaction is catalysed by an amidase which may in particular be produced by specific Rhodococcus species.Type: ApplicationFiled: June 7, 2001Publication date: January 31, 2002Inventors: Michael Ivan Page, Timothy Mark Beard
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Publication number: 20020010136Abstract: A gene coding for a mutant tyrosine repressor having increased positive regulatory activity for expression of the tyrosine phenol lyase gene compared with a tyrosine repressor not having mutation is obtained by introducing a mutation into a tyrosine repressor gene of Erwinia herbicola, introducing the gene into which the mutation is introduced into Escherichia coli expressing a lactose operon under the control of a promoter and enhancer of the tyrosine phenol lyase gene derived from Erwinia herbicola, and selecting a transformed strain having increased &bgr;-galactosidase activity.Type: ApplicationFiled: March 1, 2001Publication date: January 24, 2002Applicant: Ajinomoto Co., Inc.Inventors: Hidehiko Kumagai, Hideyuki Suzuki, Takane Katayama
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Patent number: 6338957Abstract: A method for producing halo-L-tryptophan from haloindole, comprising culturing a microorganism in a culture medium and then contacting the microorganism with (a) a mixture comprising haloindole, pyruvic acid and ammonia, or (b) a mixture comprising haloindole, a source of pyruvic acid and ammonia, until the halo-L-tryptophan is produced; and recovering the halo-L-tryptophan.Type: GrantFiled: March 8, 2001Date of Patent: January 15, 2002Assignee: Ajinomoto Co., Inc.Inventors: Norimasa Onishi, Kenzo Yokozeki
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Patent number: 6338956Abstract: A microorganism is utilized to fermentatively produce useful substances such as amino acids by cultivating the microorganism in a medium to allow a fermentative product to be produced and accumulated in the medium, and collecting the fermentative product, wherein the microorganism to be used is modified by introduction of at least one of a gene coding for a heat shock protein and a gene coding for a &sgr; factor which specifically functions for the heat shock protein gene to enhance expression amount of the heat shock protein in cells, whereby the microorganism is allowed to have added resistance to stress which would otherwise restrain growth of the microorganism and/or production of the fermentative product.Type: GrantFiled: August 8, 2000Date of Patent: January 15, 2002Assignee: Ajinomoto Co., Inc.Inventors: Eiichiro Kimura, Yoshimi Kikuchi, Yoshio Kawahara, Shinya Goto, Osamu Kurahashi, Tsuyoshi Nakamatsu
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Publication number: 20010044139Abstract: A method for producing L-phenylalanine, comprising the steps of:Type: ApplicationFiled: February 26, 2001Publication date: November 22, 2001Applicant: Ajinomoto Co., Inc.Inventors: Yurgis Antanas Vladovich Iomantas, Elena Georgievna Abalakina
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Patent number: 6316232Abstract: The invention makes available, by means of an increased provision of intracellular metabolic intermediates, in particular of erythrose 4-phosphate, alternative processes for the microbial preparation of substances, in particular of aromatic amino acids such as L-phenylalanine, in which processes the activity of a transaldolase is increased in a microorganism producing these substances. In preferred embodiments of the invention, the activity of a transketolase or the activity of a transport protein for the PEP-independent uptake of a sugar and/or the activity of a kinase which phosporylates the relevant sugar are/is additionally increased. The invention also relates to gene structures, and to transformed cells carrying these gene structures, which make it possible to implement these processes in a particularly successful manner.Type: GrantFiled: April 26, 1999Date of Patent: November 13, 2001Assignees: Holland Sweetener Company V.O.F., Forschungszentrum Julich GmbH, DSM Biotech GmbHInventors: Georg Sprenger, Ruth Siewe, Hermann Sahm, Martin Karutz, Theodorus Sonke
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Publication number: 20010029037Abstract: A method for producing halo-L-tryptophan from haloindole, comprising culturing a microorganism in a culture medium and then contacting the microorganism with (a) a mixture comprising haloindole, pyruvic acid and ammonia, or (b) a mixture comprising haloindole, a source of pyruvic acid and ammonia, until the halo-L-tryptophan is produced; and recovering the halo-L-tryptophan.Type: ApplicationFiled: March 8, 2001Publication date: October 11, 2001Applicant: AJINOMOTO CO., INC.Inventors: Norimasa Onishi, Kenzo Yokozeki
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Publication number: 20010021519Abstract: The present invention provides a catalyst system and a process for stereoselectively inverting a chiral center of a chemical compound. The catalyst system of the present invention comprises (i) a catalytic amount of a metal catalyst; (ii) an enzyme capable of oxidizing a chemical compound at a chiral center, or microorganism cells capable of producing an enzyme which is capable of oxidizing a chemical compound at a chiral center; (iii) an oxidant; and (iv) a hydrogen source. The process of the present invention comprises treating a chemical compound having a chiral center with the catalyst system of this invention.Type: ApplicationFiled: January 22, 2001Publication date: September 13, 2001Applicant: NSC Technologies, L.L.C. Delaware Limited Liability CorporationInventors: Weiguo Liu, Scott Laneman, David John Ager, Paul Phillip Taylor
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Patent number: 6280979Abstract: A microbial process for the preparation of D(-)-N-carbamoylphenylglycine from DL-5-phenylhydantoin which comprises culturing the strain pseudomonas sp. having Accession No. NCIM 5070 (ATCC 55940) and deposited in National Collection of Industrial Microorganisms (NCIM), National Chemical Laboratory (NCL) (India), having hydantoinase activity, in a medium for 16-20 hours, separating the cells by centrifugation, treating the solid cells obtained with DL-5-phenylhydantoin in buffer, at a temperature in the range of about 25-30° C. for a period of about 2-6 hours, acidifying the mixture to obtain solid D(-)-N-carbamoylphenylglycine, and separating the product by filtration.Type: GrantFiled: February 5, 1999Date of Patent: August 28, 2001Assignee: Council of Scientific & Industrial ResearchInventors: Digambar Vitthal Gokhale, Kulbhushan Balwant Bastawde, Shamrao Ganapatrao Patil, Uttam Ramrao Kalkote, Rohini Ramesh Joshi, Ramesh Anna Joshi, Thottapillil Ravindranathan, Vithal Venkatrao Jogdand, Bhaskar Ganapatrao Gaikwad, Sanjay Narayan Nene
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Patent number: 6238895Abstract: The use of strains of the genus Microbacterium for the production of organic acids or amino acids by the enzymatic conversion of a fumaric acid to the organic acid or amino acid desired, as well as methods for such use and the conversion solution produced by such use and the methods of the present invention are disclosed. The uses and the methods of the present invention provide the L-isomer form of the desired organic acid or amino acid produced thereby in the absence of the D-isomer form of the desired organic acid or amino acid. Also disclosed are reactants solutions which include the strain of the genus Microbacterium and the L-isomer form of either the organic acid or the amino acid.Type: GrantFiled: December 19, 1996Date of Patent: May 29, 2001Assignee: Genencor International, Inc.Inventor: James M. Miers
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Patent number: 6180373Abstract: A tryptophan producing strain of microorganism is selected from E. coli and Corynebacteria and is tryptophan feedback resistant and serine feedback resistant. The serine feedback resistance is by a mutation in a serA allele, where the mutated serA allele codes for a protein which has a Ki value for serine between 0.1 mM and 50 mM. The tryptophan feedback resistance is by a trpE allele which codes for a protein which has a Ki value for tryptophan between 0.1 mM and 20 mM. A process for preparing this microorganism and a process for using this microorganism are disclosed.Type: GrantFiled: April 12, 1995Date of Patent: January 30, 2001Assignee: Consortium f{umlaut over (u)}r elektrochemische Industrie GmbHInventors: G{umlaut over (u)}nter Wich, Walfred Leinfelder, Keith Backman
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Patent number: 6146859Abstract: A process for producing L-homophenylalanine comprises the step of reacting 2-oxo-4-phenylbutyric acid with a L-amino acid in the presence of tyrosine aminotransferase in a reaction solution to produce L-homophenylalanine.Type: GrantFiled: August 27, 1999Date of Patent: November 14, 2000Assignee: Academia SinicaInventors: Shui-Tein Chen, Min-Jen Tseng, Boonyaras Sookkheo
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Patent number: 6143552Abstract: The present invention provides a process for producing an L-amino acid which comprises culturing in a nutrient medium a microorganism which is capable of producing the L-amino acid and which can not grow in a synthetic medium containing said L-amino acid as the sole nitrogen source in an amount of 5 mg/ml or below, allowing the L-amino acid to accumulate in the culture, and recovering the L-amino acid from the culture.Type: GrantFiled: March 11, 1999Date of Patent: November 7, 2000Assignee: Kyowa Hakko Kogyo Co., Ltd.Inventors: Kazuyuki Okamoto, Masato Ikeda, Kuniki Kino
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Patent number: 6140088Abstract: Processes for stereoselective enzymatic conversion of certain keto carboxylic acid derivatives to form the corresponding alkylamino acid compounds are described. The invention also concerns an engineered yeast host cell containing recombinant nucleic acid capable of expressing a phenylalanine dehydrogenase, as well as an engineered host cell containing recombinant nucleic acid capable of expressing a phenylalanine dehydrogenase enzyme and nucleic acid capable of expressing a formate dehydrogenase enzyme.Type: GrantFiled: July 8, 1999Date of Patent: October 31, 2000Assignee: Bristol-Myers Squibb CompanyInventors: Ronald Hanson, Mary Jo Donovan, Steven Goldberg, Paul A. Jass, Wen-Sen Li, Ramesh Patel, Keith Ramig, Laszlo J. Szarka, John J. Venit
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Patent number: 6133018Abstract: 2-Aminopropane is used as the amine donor in the stereoselective synthesis of a chiral amine from a ketone with a transaminase. In a typical embodiment, (S)-1-methoxy-2-aminopropane is prepared by bringing methoxyacetone into contact with a transaminase in the presence of 2-aminopropane as an amine donor until a substantial amount of methoxyacetone is converted to (S)-1-methoxy-2-aminopropane and 2-aminopropane is converted to acetone. In a second embodiment, L-alanine is prepared by bringing pyruvic acid into contact with a transaminase in the presence of 2-aminopropane as an amine donor.Type: GrantFiled: March 10, 1999Date of Patent: October 17, 2000Assignee: CelgroInventors: Wei Wu, Mohit B. Bhatia, Craig M. Lewis, Wei Lang, Alice L. Wang, George W. Matcham
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Patent number: 6118047Abstract: The present invention provides a method for conferring tolerance to an amino acid analog of tryptophan to a plant and/or altering the tryptophan content of a plant by introducing and expressing an isolated DNA segment encoding an anthranilate synthase in the cells of the plant. Transgenic plants transformed with an isolated DNA segment encoding an anthranilate synthase, as well as seeds and progeny derived from these plants, are also provided. The present invention also provides a cDNA sequence of an alpha and a beta subunit of a maize anthranilate synthase.Type: GrantFiled: January 19, 1996Date of Patent: September 12, 2000Assignee: DeKalb Genetic CorporationInventors: Paul C. Anderson, Paul S. Chomet, Matthew C. Griffor, Alan L. Kriz
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Patent number: 6114163Abstract: The disclosure relates to a process for obtaining optically active L-.alpha.-aminocarboxylic acids from the corresponding racemic D,L,.alpha.-aminocarboxylic acids. The following steps are involved: (a) the D,L,.alpha.-aminocarboxylic acids are acetylated; (b) the N-acetyl-L-.alpha.-aminocarboxylic acid present in the mixture of N-acetyl-D,L,.alpha.-aminocarboxylic acids thus obtained is broken down enzymatically into the L-.alpha.-aminocarboxylic acid; (c) the L-.alpha.-aminocarboxylic acid is separated from the mixture, a quantity of a solution of N-acetyl-D(L)-.alpha.-aminocarboxylic acids and a quantity of acetate equivalent to the L-.alpha.-aminocarboxylic acid being retained; and (d) the N-acetyl-D(L)-.alpha.-aminocarboxylic acid is racemized and recycled for enzymatic breakdown. Known extraction processes involving steps (a) to (d) have the disadvantage of producing large quantities of salt.Type: GrantFiled: June 16, 1998Date of Patent: September 5, 2000Assignee: Degussa AktiengesellschaftInventors: Karlheinz Drauz, Andreas Bommarius, Michael Karrenbauer, Gunter Knaup
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Patent number: 6090588Abstract: The production of a melanin-like compound by introducing an inactivated virus into a culture of bacteria is disclosed. After introduction, the inactivated virus causes a phenotypic change in the bacteria. When introduced into a media containing tyrosine, these modified bacteria produce a melanin-like compound, pyomelanin. The bacteria will continue to produce pyomelanin until they are removed from the tyrosine source.Type: GrantFiled: April 29, 1998Date of Patent: July 18, 2000Assignee: Board of Regents of University of NebraskaInventors: Tyler A. Kokjohn, John O. Schrader
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Patent number: 6087136Abstract: A microbial process for the preparation of D(-)-N-carbamoylphenylglycine from DL-5-phenylhydantoin which comprises culturing the strain pseudomonas sp. having Accession No. NCIM 5070 (ATCC 55940) and deposited in National Collection of Industrial microorganisms (NCIM), National Chemical laboratory (NCL) (India), having hydantoinase activity, in a medium for 16-20 hours, separating the cells by centrifugation, treating the solid cells obtained with DL-5-phenylhydantoin in buffer, at a temperature in the range of about 25-30.degree. C. for a period of about 2-6 hours, acidifying the mixture to obtain solid D(-)-N-carbamoylphenylglycine, and separating the product by filtration.Type: GrantFiled: March 31, 1997Date of Patent: July 11, 2000Assignee: Council of Scientific & Industrial ResearchInventors: Digambar Vitthal Gokhale, Kulbhushan Balwant Bastawde, Shamrao Ganapatrao Patil, Uttam Ramrao Kalkote, Rohini Ramesh Joshi, Ramesh Anna Joshi, Thottapillil Ravindranathan, Vithal Venkatrao Jogdand, Bhaskar Ganapatrao Gaikwad, Sanjay Narayan Nene
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Patent number: 6063615Abstract: Disclosed is a process for preparing acylated amino esters and a process for preparing optically active amino esters from racemic amino esters with a carboxylic ester as acylating agent, whose acid component has a halogen, nitrogen, oxygen or sulfur atom neighboring the carbonyl carbon atom, in the presence of a hydrolase selected from the group of amidase, protease, esterase and lipase, and subsequent separation of the enantioselectively acylated amino ester from the non-acylated other enantiomer of the amino ester.Type: GrantFiled: June 25, 1998Date of Patent: May 16, 2000Assignee: BASF AktiengesellschaftInventors: Rainer Sturmer, Klaus Ditrich, Wolfgang Siegel
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Patent number: 6040330Abstract: A pharmaceutical formulation of a taxane antineoplastic agent, particularly paclitaxel or docetaxel or a pharmaceutically acceptable salt thereof, and N-methylpyrrolidin-2-one (NMP). The formulation may include other excipients and/or diluents, and is suitable for administration to patients with cancer.Type: GrantFiled: January 8, 1999Date of Patent: March 21, 2000Assignee: BioNumerik Pharmaceuticals, Inc.Inventors: Fredrick H. Hausheer, Dhanabalan Murali, Peddaiahgari Seetharamulu
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Patent number: 6015698Abstract: D-amino acid with high optical purity represented by formula (1-A) and/or formula (1-B), ##STR1## wherein R represents H or OH, ##STR2## wherein R.sub.1, R.sub.2 each represents H or OH, and amine represented by formula (2-A) and/or formula (2-B) ##STR3## wherein R represents H or OH, ##STR4## wherein R.sub.1 and R.sub.2 each represents H or OH, can be produced economically in an industrial scale by contacting a mixture of enantiomers of amino acid represented by the above formula (1-A) and/or formula (1-B) with a microorganism capable of selectively degrading L-amino acid or with at least one of the treated products of the microorganism.Type: GrantFiled: February 13, 1998Date of Patent: January 18, 2000Assignee: Daicel Chemical Industries, Ltd.Inventor: Teruyuki Nikaido
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Patent number: 5985617Abstract: Genetic elements comprising expression vectors and a gene coding for phosphoenol pyruvate synthase is utilized to enhance diversion of carbon resources into the common aromatic pathway and pathways branching therefrom. The overexpression of phosphoenol pyruvate synthase increases DAHP production to near theoretical yields.Type: GrantFiled: March 26, 1999Date of Patent: November 16, 1999Inventor: James C. Liao
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Patent number: 5981239Abstract: A biocatalytic process to produce optically active phenylalanine analogs from arylacrylic acids is disclosed in which Rhodotorula graminis yeast containing phenylalanine ammonia-lyase introduces a molecule of ammonia stereoselectively onto the double bond of a 3-substituted acrylic acid. The substituent at the 3-position of the 3-substituted acrylic acid includes, for example, aromatic rings such as substituted phenyl groups, five member aromatic heterocyclics, and six member aromatic heterocyclics.Type: GrantFiled: September 24, 1997Date of Patent: November 9, 1999Assignee: Great Lakes Chemical Corp.Inventor: Weiguo Liu
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Patent number: 5939295Abstract: An improved recombinant DNA plasmid composed of a DNA vector and other DNA fragments containing the tryptophan operon from E. coli, the aroG gene from E. coli and the serA gene from E. coli is shown. The plasmid is transformed into a microorganism belonging to Escherichia coli, and the microorganisms are cultured in a medium and the L-tryptophan accumulated in the culture is recovered.Type: GrantFiled: March 29, 1996Date of Patent: August 17, 1999Assignee: Archer Daniels Midland CompanyInventors: Mark S. Dunkak, Richard D. Dancey, Thomas W. McMullin, Paul D. Hanke
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Patent number: 5919670Abstract: The present invention provides a process for producing an L-amino acid which comprises culturing in a nutrient medium a microorganism which is capable of producing the L-amino acid and which can not grow in a synthetic medium containing said L-amino acid as the sole nitrogen source in an amount of 5 mg/ml or below, allowing the L-amino acid to accumulate in the culture, and recovering the L-amino acid from the culture.Type: GrantFiled: June 18, 1998Date of Patent: July 6, 1999Assignee: Kyowa Hakko Kogyo Co., Ltd.Inventors: Kazuyuki Okamoto, Masato Ikeda, Kuniki Kino
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Patent number: 5916781Abstract: The method for producing D-tryptophan with high optical purity and high yield is provided, which comprises contacting a mixture of D,L-tryptophan with organisms which produce tryptophanase to degrade L-tryptophan selectively, thereby increasing the content of D-tryptophan in D,L-tryptophan.Type: GrantFiled: January 9, 1998Date of Patent: June 29, 1999Assignee: Daicel Chemical Industries, Ltd.Inventors: Hiroaki Yamamoto, Kazuya Mitsuhashi, Akinobu Matsuyama, Fusao Tomita
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Patent number: 5906925Abstract: Genetic elements comprising expression vectors and a gene coding for phosphoenol pyruvate synthase is utilized to enhance diversion of carbon resources into the common aromatic pathway and pathways branching therefrom. The overexpression of phosphoenol pyruvate synthase increases DAHP production to near theoretical yields.Type: GrantFiled: February 18, 1997Date of Patent: May 25, 1999Inventor: James C. Liao