Nitrogen As Only Ring Hetero Atom Patents (Class 435/121)
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Patent number: 11518975Abstract: The present disclosure relates to a non-naturally occurring microorganism that includes an endogenous genetic deletion that eliminates the expression of at least a pyruvate kinase, where the genetically modified prokaryotic microorganism is capable of producing 3-deoxy-D-arabino-heptulosonate-7-phosphate.Type: GrantFiled: April 30, 2019Date of Patent: December 6, 2022Assignees: Alliance for Sustainable Energy, LLC, UT-Battelle, LLC, TRIAD National Security, LLCInventors: Christopher W. Johnson, Peter Corbin St. John, Gregg Tyler Beckham, Joshua Ryan Elmore, Adam Michael Guss, Davinia Salvachua Rodriguez, Gayle Joann Bentley, George Lee Peabody, V, Taraka Dale, Ramesh K. Jha, Niju Narayanan
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Patent number: 11505564Abstract: This invention relates to the large-scale production of psilocybin for use in medicine. More particularly, it relates to a method of obtaining high purity crystalline psilocybin, particularly, in the form of Polymorph A. It further relates to a method for the manufacture of psilocybin and intermediates in the production thereof and formulations containing psilocybin.Type: GrantFiled: November 8, 2019Date of Patent: November 22, 2022Assignee: COMPASS PATHFINDER LIMITEDInventors: Derek John Londesbrough, Christopher Brown, Julian Scott Northen, Gillian Moore, Hemant Kashinath Patil, David E. Nichols
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Patent number: 11479758Abstract: Methods for evolving cells or strains towards improved methyltransferase activity, particularly SAM-dependent methyltransferase activity, as well as to cells and strains useful in such methods and methods of using the evolved cells in the production of methylated products.Type: GrantFiled: August 24, 2017Date of Patent: October 25, 2022Assignee: Danmarks Tekniske UniversitetInventors: Hao Luo, Anne Sofie Laerke Hansen
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Patent number: 11293026Abstract: The invention provides a non-naturally occurring microbial organism having an adipate, 6-aminocaproic acid or caprolactam pathway. The microbial organism contains at least one exogenous nucleic acid encoding an enzyme in the respective adipate, 6-aminocaproic acid or caprolactam pathway. The invention additionally provides a method for producing adipate, 6-aminocaproic acid or caprolactam. The method can include culturing an adipate, 6-aminocaproic acid or caprolactam producing microbial organism, where the microbial organism expresses at least one exogenous nucleic acid encoding an adipate, 6-aminocaproic acid or caprolactam pathway enzyme in a sufficient amount to produce the respective product, under conditions and for a sufficient period of time to produce adipate, 6-aminocaproic acid or caprolactam.Type: GrantFiled: August 23, 2019Date of Patent: April 5, 2022Assignee: Genomatica, Inc.Inventors: Anthony P. Burgard, Priti Pharkya, Robin E. Osterhout
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Patent number: 11124811Abstract: A method of producing ?-hydromuconic acid using a metabolic pathway of a microorganism is disclosed. The method of producing ?-hydromuconic acid includes the step of culturing at least one type of microorganism having a capacity to produce ?-hydromuconic acid, selected from the group consisting of microorganisms belonging to the genus Escherichia, microorganisms belonging to the genus Pseudomonas, microorganisms belonging to the genus Hafnia, microorganisms belonging to the genus Bacillus, microorganisms belonging to the genus Cupriavidus, microorganisms belonging to the genus Acinetobacter, microorganisms belonging to the genus Alcaligenes, microorganisms belonging to the genus Delftia, and microorganisms belonging to the genus Shimwellia.Type: GrantFiled: June 9, 2016Date of Patent: September 21, 2021Assignee: TORAY INDUSTRIES, INC.Inventors: Kyohei Isobe, Kenji Kawamura, Masateru Ito, Katsushige Yamada
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Patent number: 11104907Abstract: The invention relates to engineering of acetyl-CoA metabolism in yeast and in particular to production of acetyl-CoA in a non-ethanol producing yeast lacking endogenous gene(s) encoding pyruvate decarboxylase and comprising a heterologous pathway for synthesis of cytosolic acetyl-CoA.Type: GrantFiled: June 12, 2020Date of Patent: August 31, 2021Assignee: BIOPETROLIA ABInventors: Jens Nielsen, Verena Siewers, Anastasia Krivoruchko, Yiming Zhang, Zongjie Dai
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Patent number: 10858677Abstract: A novel method of producing 3-hydroxyadipic acid using a metabolic pathway of a microorganism is disclosed.Type: GrantFiled: June 9, 2016Date of Patent: December 8, 2020Assignee: TORAY INDUSTRIES, INC.Inventors: Kyohei Isobe, Kenji Kawamura, Masateru Ito, Katsushige Yamada
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Patent number: 10577634Abstract: Embodiments of the present invention relate to methods for the biosynthesis of di- or trifunctional C7 alkanes in the presence of isolated enzymes or in the presence of a recombinant host cell expressing those enzymes. The di- or trifunctional C7 alkanes are useful as intermediates in the production of nylon-7, nylon-7,x, nylon-x,7, and polyesters.Type: GrantFiled: March 20, 2017Date of Patent: March 3, 2020Assignee: INVISTA NORTH AMERICA S.A.R.L.Inventors: Paul S. Pearlman, Changlin Chen, Adriana L. Botes, Alex Van Eck Conradie, Benjamin D. Herzog
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Patent number: 10308623Abstract: Processes for preparing tetrahydrofuran and/or butane-1,4-diol and/or gamma-butyrolactone are provided, including a process for preparing tetrahydrofuran (THF) from succinic acid that has been obtained by conversion of biomass, by conversion of the succinic acid to succinic anhydride, and hydrogenation of the succinic anhydride, with removal of certain secondary components.Type: GrantFiled: January 8, 2016Date of Patent: June 4, 2019Assignee: BASF SEInventors: Alexander Duefert, Rolf Pinkos, Wolf-Steffen Weissker
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Patent number: 10221134Abstract: The present invention provides a scalable synthesis of enantiomerically pure brivaracetam, and related derivatives.Type: GrantFiled: May 24, 2016Date of Patent: March 5, 2019Assignee: Esteve Quimica S.A.Inventors: Peng Wang, Pixu Li, Qiang Wei, Yuanhua Liu
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Patent number: 10150977Abstract: The invention provides a non-naturally occurring microbial organism having a 6-aminocaproic acid, caprolactam, hexametheylenediamine or levulinic acid pathway. The microbial organism contains at least one exogenous nucleic acid encoding an enzyme in the respective 6-aminocaproic acid, caprolactam, hexametheylenediamine or levulinic acid pathway. The invention additionally provides a method for producing 6-aminocaproic acid, caprolactam, hexametheylenediamine or levulinic acid. The method can include culturing a 6-aminocaproic acid, caprolactam or hexametheylenediamine producing microbial organism, where the microbial organism expresses at least one exogenous nucleic acid encoding a 6-aminocaproic acid, caprolactam, hexametheylenediamine or levulinic acid pathway enzyme in a sufficient amount to produce the respective product, under conditions and for a sufficient period of time to produce 6-aminocaproic acid, caprolactam, hexametheylenediamine or levulinic acid.Type: GrantFiled: September 12, 2016Date of Patent: December 11, 2018Assignee: Genomatica, Inc.Inventors: Mark J. Burk, Anthony P. Burgard, Robin E. Osterhout, Priti Pharkya
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Patent number: 10047363Abstract: A nucleic acid molecule comprises a nucleotide sequence: as shown in SEQ ID No. 1, which is the complement of SEQ ID No. 1, which is degenerate with SEQ ID No. 1, or which has at least 85% sequence identity with SEQ ID No. 1, or which is a part of such a sequence. The nucleic acid molecule encodes or is a complementary to a nucleic acid molecule encoding one or more polypeptides, or comprises or is complementary to a nucleic acid molecule comprising one or more genetic elements, having functional activity in the synthesis of a polyketide-based or macrolactam molecule. The nucleic acid molecule may be used to prepare a modified BE-14106 biosynthetic gene cluster for the preparation of a modified BE-14106 molecule.Type: GrantFiled: November 11, 2015Date of Patent: August 14, 2018Assignee: SINTEF TTO ASInventors: Hanne Jørgensen, Havard Sletta, Trond Erling Ellingsen, Espen Fjaervik, Kristin Fløgstad Degnes, Geir Klinkenberg, Per Bruheim, Sergey Zotchev
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Patent number: 9937478Abstract: Materials such as biomass (e.g., plant biomass, animal biomass, and municipal waste biomass) and hydrocarbon-containing materials are processed to produce useful products, such as fuels. For example, systems are described that can use feedstock materials, such as cellulosic and/or lignocellulosic materials and/or starchy materials, or oil sands, oil shale, tar sands, bitumen, and coal to produce altered materials such as fuels (e.g., ethanol and/or butanol). The processing includes exposing the materials to an ion beam.Type: GrantFiled: May 22, 2017Date of Patent: April 10, 2018Assignee: XYLECO, INC.Inventor: Marshall Medoff
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Patent number: 9809833Abstract: Recombinant microorganisms comprising at least one exogenous nucleic acid sequence and capable of producing adipate semialdehyde are provided. Adipate semialdehyde may be produced in a synthesis pathway utilizing a single thiolase reaction. Adipate semialdehyde may also be produced from intermediates consisting of alpha, omega difunctional aliphatic organic molecules. Methods of using recombinant microorganisms to produce 6-aminocaproic acid, adipic acid, hexamethylenediamine and 1.6-hexanediol are also provided.Type: GrantFiled: September 20, 2013Date of Patent: November 7, 2017Assignee: BioAmber Inc.Inventors: Man Kit Lau, Christopher P. Mercogliano
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Patent number: 9631211Abstract: The present invention relates to a novel bacterial strain designated DD1, which has the ability to produce organic acids, in particular succinic acid (SA), which was originally isolated from bovine rumen, and is capable of utilizing glycerol as a carbon source; and variant strains derived there from retaining said capability; as well as to methods of producing organic acids, in particular succinic acid by making use of said microorganism.Type: GrantFiled: October 3, 2013Date of Patent: April 25, 2017Assignee: BASF SEInventors: Edzard Scholten, Dirk Dagele, Stephan Haefner, Hartwig Schroder
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Patent number: 9308175Abstract: One aspect of the invention relates to a pharmaceutical dosage unit for sublingual, buccal, pulmonary or oral administration, said dosage unit having a weight of 20-500 mg and comprising 1-80 wt. % of a microgranulate that is distributed throughout a solid hydrophilic matrix; said microgranulate being characterized in that it: has a volume weighted average diameter of 5-100 m; contains at least 0.01 wt. %, preferably at least 0.1 wt. % of one or more water-insoluble pharmaceutically active substances; contains at least 10 wt. %, preferably at least 20 wt. % of an emulsifier component; and is capable of forming a micro-emulsion upon contact with saliva or water. The dosage units of the present invention achieve the inherent benefits of oral delivery while at the same time realizing a high transmucosal absorption rate of the cannabinoids contained therein.Type: GrantFiled: September 14, 2007Date of Patent: April 12, 2016Assignee: Echo Pharmaceuticals B.V.Inventors: Hubert Clemens Pellikaan, Pieter Sebastiaan Vermeulen, Johannes Caspar Mathias Elizabeth Bender, Geert Feye Woerlee
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Patent number: 9290755Abstract: This invention relates to polypeptides having aldolase activity, including pyruvate activity such as, without limitation, HMG and/or KHG aldolase activity, polynucleotides encoding these polypeptides, and methods of making and using these polynucleotides and polypeptides. In some embodiments, the invention is directed to polypeptides having aldolase activity, including pyruvate activity such as, without limitation, HMG and/or KHG aldolase activity, including thermostable and thermotolerant activity, and polynucleotides encoding these enzymes, and making and using these polynucleotides and polypeptides. The polypeptides in accordance with the invention can be used in a variety of pharmaceutical, agricultural and industrial contexts.Type: GrantFiled: August 14, 2013Date of Patent: March 22, 2016Assignee: BASF Enzymes LLCInventors: David P. Weiner, Lishan Zhao, Peter Luginbuhl, Ellen Burke, Toby H. Richardson, Paula M. Hicks
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Patent number: 9150845Abstract: This invention provides polypeptides having lyase activity, polynucleotides encoding these polypeptides, and methods of making and using these polynucleotides and polypeptides. In one aspect, the invention is directed to polypeptides having ammonia lyase activity, e.g., phenylalanine ammonia lyase, tyrosine ammonia lyase and/or histidine ammonia lyase activity, including thermostable and thermotolerant activity, and polynucleotides encoding these enzymes, and making and using these polynucleotides and polypeptides. The polypeptides of the invention can be used in a variety of pharmaceutical, agricultural and industrial contexts.Type: GrantFiled: August 30, 2013Date of Patent: October 6, 2015Assignee: BASF Enzymes LLCInventors: David P. Weiner, Alexander Varvak, Toby Richardson, Mircea Podar, Ellen Burke, Shaun Healey
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Patent number: 9102963Abstract: The present disclosure relates to biocatalysts and its uses for the efficient preparation of eslicarbazepine, eslicarbazepine acetate, and analogs thereof.Type: GrantFiled: April 12, 2012Date of Patent: August 11, 2015Assignee: Codexis, Inc.Inventors: Anupam Gohel, Derek Smith, Brian Wong, Joly Sukumaran, Wan Lin Yeo, Steven J. Collier, Scott Novick
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Patent number: 9102959Abstract: The disclosure relates to engineered ketoreductase polypeptides and processes of using the polypeptides for production of phenylephrine.Type: GrantFiled: August 19, 2010Date of Patent: August 11, 2015Assignee: Codexis, Inc.Inventors: Oscar Alvizo, Steven J. Collier, Joerg Hennemann, Seong Ho Oh, Wenjuan Zha
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Patent number: 9040262Abstract: The present disclosure relates to non-naturally occurring polypeptides useful for preparing Ezetimibe, polynucleotides encoding the polypeptides, and methods of using the polypeptides.Type: GrantFiled: May 4, 2011Date of Patent: May 26, 2015Assignee: Codexis, Inc.Inventors: Michael Crowe, Oscar Alvizo, Behnaz Behrouzian, Yong Koy Bong, Steven J. Collier, Anupam Gohel, Jagadeesh Mavinahalli, Naga Modukuru, Emily Mundorff, Derek Smith, Shiwei Song, Wan Lin Yeo
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Patent number: 9034610Abstract: Methods and compositions that can be used to make monatin from glucose, tryptophan, indole-3-lactic acid, indole-3-pyruvate, and 2-hydroxy 2-(indol-3-ylmethyl)-4-keto glutaric acid, are provided. Methods are also disclosed for producing the indole-3-pyruvate and 2-hydroxy 2-(indol-3-ylmethyl)-4-keto glutaric acid intermediates. Compositions provided include nucleic acid molecules, polypeptides, chemical structures, and cells. Methods include in vitro and in vivo processes, and the in vitro methods include chemical reactions.Type: GrantFiled: April 4, 2013Date of Patent: May 19, 2015Assignee: CARGILL, INCORPORATEDInventors: Timothy W. Abraham, Douglas C. Cameron, Paula M. Hicks, Sara C. McFarlan, James R. Millis, John Rosazza, David P. Weiner, Lishan Zhao
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Publication number: 20150132806Abstract: The present disclosure provides engineered ketoreductase enzymes having improved properties as compared to a naturally occurring wild-type ketoreductase enzyme including the capability of reducing 5-((4S)-2-oxo-4-phenyl(1,3-oxazolidin-3-yl))-1-(4-fluorophenyl)pentane-1,5-dione to (4S)-3-[(5S)-5-(4-fluorophenyl)-5-hydroxypentanoyl]-4-phenyl-1,3-oxazolidin-2-one. Also provided are polynucleotides encoding the engineered ketoreductase enzymes, host cells capable of expressing the engineered ketoreductase enzymes, and methods of using the engineered ketoreductase enzymes to synthesize the intermediate (4S)-3-[(5S)-5-(4-fluorophenyl)-5-hydroxypentanoyl]-4-phenyl-1,3-oxazolidin-2-one in a process for making Ezetimibe.Type: ApplicationFiled: January 27, 2015Publication date: May 14, 2015Inventors: Emily Mundorff, Erik Jan De Vries
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Publication number: 20150132807Abstract: The present application provides engineered polypeptides having imine reductase activity, polynucleotides encoding the engineered imine reductases, host cells capable of expressing the engineered imine reductases, and methods of using these engineered polypeptides with a range of ketone and amine substrate compounds to prepare secondary and tertiary amine product compounds.Type: ApplicationFiled: November 12, 2014Publication date: May 14, 2015Inventors: Nicholas J. Agard, Oscar Alvizo, Melissa Ann Mayo, Stefanie Ng Minor, James Nicholas Riggins, Jeffrey C. Moore
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Publication number: 20150111260Abstract: This invention relates to polypeptides having aldolase activity, including pyruvate activity such as, without limitation, HMG and/or KHG aldolase activity, polynucleotides encoding these polypeptides, and methods of making and using these polynucleotides and polypeptides. In some embodiments, the invention is directed to polypeptides having aldolase activity, including pyruvate activity such as, without limitation, HMG and/or KHG aldolase activity, including thermostable and thermotolerant activity, and polynucleotides encoding these enzymes, and making and using these polynucleotides and polypeptides. The polypeptides in accordance with the invention can be used in a variety of pharmaceutical, agricultural and industrial contexts.Type: ApplicationFiled: October 28, 2014Publication date: April 23, 2015Inventors: Ellen BURKE, Steven John GORT, Paula M. HICKS, Peter LUGINBUHL, Sara C. MCFARLAN, Toby RICHARDSON, Christopher SOLHEID, David P. WEINER, Lishan ZHAO
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Patent number: 9005938Abstract: This invention relates to polypeptides having aldolase activity, including pyruvate activity such as, without limitation, HMG and/or KHG aldolase activity, polynucleotides encoding these polypeptides, and methods of making and using these polynucleotides and polypeptides. In some embodiments, the invention is directed to polypeptides having aldolase activity, including pyruvate activity such as, without limitation, HMG and/or KHG aldolase activity, including thermostable and thermotolerant activity, and polynucleotides encoding these enzymes, and making and using these polynucleotides and polypeptides. The polypeptides in accordance with the invention can be used in a variety of pharmaceutical, agricultural and industrial contexts.Type: GrantFiled: September 16, 2011Date of Patent: April 14, 2015Assignee: Cargill, IncorporatedInventors: Ellen Burke, Steven John Gort, Paula M. Hicks, Peter Luginbuhl, Sara C. McFarlan, Toby Richardson, Christopher Solheid, David Weiner, Lishan Zhao
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Publication number: 20150099276Abstract: The present invention relates to the use of nucleic acid molecules coding for a bacterial xylose isomerase (XI), preferably coming from Clostridium phytofermentans, for reaction/metabolization, particularly fermentation, of recombinant microorganisms of biomaterial containing xylose, and particularly for the production of bioalcohols, particularly bioethanol, by means of xylose fermenting yeasts. The present invention further relates to cells, particularly eukaryotic cells, which are transformed utilizing a nucleic acid expression construct which codes for a xylose isomerase, wherein the expression of the nucleic acid expression construct imparts to the cells the capability to directly isomerize xylose into xylulose. Said cells are preferably utilized for reaction/metabolization, particularly fermentation, of biomaterial containing xylose, and particularly for the production of bioalcohols, particularly bioethanol.Type: ApplicationFiled: December 16, 2014Publication date: April 9, 2015Inventors: Dawid BRAT, Eckhard BOLES, Marco KELLER, Beate ROTHER
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Patent number: 8986948Abstract: The present invention relates to the use of nucleic acid molecules coding for a bacterial xylose isomerase (XI), preferably coming from Clostridium phytofermentans, for reaction/metabolization, particularly fermentation, of recombinant microorganisms of biomaterial containing xylose, and particularly for the production of bioalcohols, particularly bioethanol, by means of xylose fermenting yeasts. The present invention further relates to cells, particularly eukaryotic cells, which are transformed utilizing a nucleic acid expression construct which codes for a xylose isomerase, wherein the expression of the nucleic acid expression construct imparts to the cells the capability to directly isomerize xylose into xylulose. Said cells are preferably utilized for reaction/metabolization, particularly fermentation, of biomaterial containing xylose, and particularly for the production of bioalcohols, particularly bioethanol.Type: GrantFiled: July 1, 2009Date of Patent: March 24, 2015Assignee: Lesaffre et CompagnieInventors: Dawid Brat, Eckhard Boles, Marco Keller, Beate Wiedemann
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Publication number: 20150079640Abstract: The present disclosure relates to methods of using transaminase polypeptides in the synthesis of chiral amines from prochiral ketones.Type: ApplicationFiled: November 19, 2014Publication date: March 19, 2015Inventors: Gregory Hughes, Paul N. Devine, Fred J. Fleitz, Brendan T. Grau, John Limanto, Christopher Savile, Emily Mundorff
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Publication number: 20150072383Abstract: The present disclosure provides engineered transaminase polypeptides for the production of amines, polynucleotides encoding the engineered transaminases, host cells capable of expressing the engineered transaminases, and methods of using the engineered transaminases to prepare compounds useful in the production of active pharmaceutical agents. The present disclosure provides engineered polypeptides having transaminase activity, polynucleotides encoding the polypeptides, methods of the making the polypeptides, and methods of using the polypeptides for the biocatalytic conversion of ketone substrates to amine products. The present enzymes have been engineered to have one or more residue differences as compared to the amino acid sequence of the naturally occurring transaminase of Vibrio fluvialis. In particular, the transaminases of the present disclosure have been engineered for efficient formation of chiral tryptamine derivatives from its corresponding prochiral ketone substrates.Type: ApplicationFiled: March 22, 2013Publication date: March 12, 2015Inventors: Jovana Nazor, Derek Smith, Michael Crowe, Shiwei Song, Steven J. Collier
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Patent number: 8975046Abstract: Monatin and certain stereoisomers of monatin, such as R,R monatin and S,R monatin, as well as salts thereof, are produced using polypeptides and biosynthetic pathways. These polypeptides and biosynthetic pathways are also useful in the production of R-2-hydroxy-2-(indoly-3-ylmethyl)-4-keto glutaric acid, an intermediate that is formed in certain monatin synthesis pathways, including some biosynthetic pathways.Type: GrantFiled: November 2, 2011Date of Patent: March 10, 2015Assignee: Cargill, IncorporatedInventors: Brian J. Brazeau, Mervyn L. De Souza, Steven J. Gort, Paula M. Hicks, Sherry R. Kollmann, Jose M. Laplaza, Sara C. McFarlan, Fernando A. Sanchez-Riera, Christopher Solheid
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Publication number: 20150045562Abstract: The present invention relates to processes and intermediates useful for the manufacture of spiroindolone compounds such as (1?R,3?S)-5,7?-dichloro-6?-fluoro-3?-methyl-2?,3?,4?,9?-tetrahydrospiro[indoline-3,1?-pyrido[3,4-b]indol]-2-one and salts and hydrates and solvates thereof.Type: ApplicationFiled: March 22, 2013Publication date: February 12, 2015Applicant: Novartis AGInventors: Michael Crowe, Michael Foulkes, Giancarlo Francese, Dominique Grimler, Ernst Kuesters, Kurt Laumen, Yunzhong Li, Changxue Lin, Jovana Nazor, Thomas Ruch, Derek Smith, Shiwei Song, Shangjun Teng
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Publication number: 20150031076Abstract: The present invention relates to host cells transformed with a nucleic acid sequence encoding a eukaryotic xylose isomerase obtainable from an anaerobic fungus. When expressed, the sequence encoding the xylose isomerase confers to the host cell the ability to convert xylose to xylulose which may be further metabolized by the host cell. Thus, the host cell is capable of growth on xylose as carbon source. The host cell preferably is a eukaryotic microorganism such as a yeast or a filamentous fungus. The invention further relates to processes for the production of fermentation products such as ethanol, in which a host cell of the invention uses xylose for growth and for the production of the fermentation product. The invention further relates to nucleic acid sequences encoding eukaryotic xylose isomerases and xylulose kinases as obtainable from anaerobic fungi.Type: ApplicationFiled: October 2, 2014Publication date: January 29, 2015Inventors: Hubertus Johannes Marie OP DEN CAMP, Harry Ramanoedj HARHANGI, Christiaan VAN DER DRIFT, Jacobus Thomas PRONK
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Publication number: 20150024440Abstract: Recombinant microbial cells and methods for producing melatonin and related compounds using such cells are described. More specifically, the recombinant microbial cell may comprise exogenous genes encoding one or more of an L-tryptophan hydroxylase, a 5-hydroxy-L-tryptophan decarboxylyase, a serotonin acetyltransferase, an acetylserotonin O-methyltransferase; an L-tryptophan decarboxy-lyase, and a tryptamine-5-hydroxylase, and means for providing tetrahydrobiopterin (THB). Related sequences and vectors for use in preparing such recombinant microbial cells are also described.Type: ApplicationFiled: February 28, 2013Publication date: January 22, 2015Inventors: Eric Michael Knight, Jiangfeng Zhu, Jochen Förster, Hao Luo
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Publication number: 20150017683Abstract: Scalable biomaterial-based bioreactors are described. In one embodiment, the bioreactor may comprise perforated plates stacked such that the assembled bioreactor has the necessary manifolds and chambers to transport gas and liquids to a biomaterial contained within the bioreactor, and to remove the reaction products. In another embodiment, single use bioreactors are described. Methods of operating the bioreactors are also described.Type: ApplicationFiled: December 19, 2012Publication date: January 15, 2015Inventors: Zia Abdullah, Michael L. Dickens, Micah Paul McCreery, Randy L. Jones, Elvin Ray Beach, III, Jon-David S. Sears, Erin Suzanne Schultz, Stephanie Ann Smith, Paul E. George, II
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Publication number: 20150010943Abstract: The present invention relates to genetic modifications in eukaryotic host cells that have been transformed to express a xylose isomerase that confers on the host cell the ability to isomerize xylose to xylulose. These genetic modifications are aimed at improving the efficiency of xylose metabolism and include, e.g., reduction of nonspecific aldose reductase activity, increased xylulose kinase activity and increased flux of the pentose phosphate pathway. The modified host cells of the invention are suitable for the production of a wide variety of fermentation products, including ethanol, in fermentation processes in which a source of xylose or a source of xylose and glucose are used as carbon source.Type: ApplicationFiled: June 24, 2014Publication date: January 8, 2015Inventors: Aaron Adriaan WINKLER, Sipko Maarten KUYPER, Wilhelmus Theodorus Antonius Maria DE LAAT, Johannes Pieter VAN DIJKEN, Jacobus Thomas PRONK
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Publication number: 20150004660Abstract: Embodiments of the invention relate to the enzymatic conversion of bioderived feedstocks to commercially valuable chemicals. The enzymatic conversions of the embodiments of the invention offer the potential for lower cost routes to these value-added chemicals. Some of the chemicals that are useful include nylon intermediates such as caprolactam, adipic acid, 1,6-hexamethylene diamine; butanediols such as 1,4-butanediol, 1,3-butanediol, and 2,3-butanediol; butanols such as 1-butanol, and 2-butanol; succinic acid, butadiene, isoprene, and 3-hydroxypropanoic acid.Type: ApplicationFiled: September 18, 2014Publication date: January 1, 2015Inventors: Paul S. Pearlman, Changlin Chen, Adriana Leonora Botes
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Patent number: 8921079Abstract: The present disclosure relates to methods of using transaminase polypeptides in the synthesis of chiral amines from prochiral ketones.Type: GrantFiled: June 21, 2010Date of Patent: December 30, 2014Assignee: Codexis, Inc.Inventors: Gregory Hughes, Paul N. Devine, Fred J. Fleitz, Brendan T. Grau, John Limanto, Christopher Savile, Emily Mundorff
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Publication number: 20140377783Abstract: A novel type of dye systems comprises a selection of 10H-indolo[1,2-a]indole compounds (henceforth abbreviated as IO compounds) and (5H,7H)-indolo[1,2-a]quinoline compounds (henceforth abbreviated as IQ compounds) showing a solvatochromic effect and exhibiting strong fluorescence in a variety of materials such as polypropylene, polyethylene, oils, various solvents, emulsions. Also disclosed are various methods how the IO/IQ compounds can be administered, especially how they can be produced and administered in situ from a precursor, responding to external stimuli such as enzyme activity, temperature and so forth. The response of a precursor to external stimuli can also be used to determine the presence or absence of such stimuli.Type: ApplicationFiled: November 4, 2011Publication date: December 25, 2014Inventors: Lukas Wick, Urs Spitz, Christophe Weymuth, Günter Schabert, Thomas Mayer, Alexander Bayer
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Publication number: 20140370552Abstract: The present invention provides endoglucanase 1b (EG1b) variants suitable for use in saccharification reactions. The present application further provides genetically modified fungal organisms that produce EG1b variants, as well as enzyme mixtures exhibiting enhanced hydrolysis of cellulosic material to fermentable sugars, enzyme mixtures produced by the genetically modified fungal organisms, and methods for producing fermentable sugars from cellulose using such enzyme mixtures.Type: ApplicationFiled: December 18, 2012Publication date: December 18, 2014Inventors: Ee Lui Ang, Ellen D. Eberhard, Xiyun Zhang, Wei Zhang, Jing Tian, Derek J. Smith, Vesna Mitchell
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Publication number: 20140357690Abstract: The present invention relates to processes and intermediates useful in the preparation of (R)-2-(7-(4-cyclopentyl-3-(trifluoromethyl)benzyloxy)-1,2,3,4-tetrahydrocyclopenta[b]indol-3-yl)acetic acid of Formula (Ia) and salts thereof, an S1P1 receptor modulator that is useful in the treatment of S1P1 receptor-associated disorders, for example, diseases and disorders mediated by lymphocytes, transplant rejection, autoimmune diseases and disorders, inflammatory diseases and disorders (e.g., acute and chronic inflammatory conditions), cancer, and conditions characterized by an underlying defect in vascular integrity or that are associated with angiogenesis such as may be pathologic (e.g., as may occur in inflammation, tumor development and atherosclerosis).Type: ApplicationFiled: August 20, 2014Publication date: December 4, 2014Applicant: ARENA PHARMACEUTICALS, INC.Inventors: Antonio Garrido Montalban, Daniel J. Buzard, John A. DeMattei, Tawfik Gharbaoui, Stephen R. Johannsen, Ashwin M. Krishnan, Young Mi Kuhlman, You-An Ma, Michael John Martinelli, Suzanne Michiko Sato, Dipanjan Sengupta
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Patent number: 8900830Abstract: A process for efficiently producing optically active succinimide derivatives as key intermediates of (3R)-2?-(4-bromo-2-fluorobenzyl)spiro{pyrrolidine-3,4?(1?H)-pyrrolo[1,2-a]pyrazine}-1?,2,3?,5(2?H)-tetraone, which comprises the following reaction steps, and the step 2 is performed by using a non-animal-derived enzyme.Type: GrantFiled: July 28, 2011Date of Patent: December 2, 2014Assignees: Sumitomo Dainippon Pharma Co., Ltd., Kyowa Hakko Bio Co., Ltd.Inventors: Shinji Kita, Kazuya Tsuzaki, Eitora Yamamura
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Publication number: 20140329916Abstract: Provided herein is a non-naturally occurring microbial organism having a methanol metabolic pathway that can enhance the availability of reducing equivalents in the presence of methanol. Such reducing equivalents can be used to increase the product yield of organic compounds produced by the microbial organism, such as adipate, 6-aminocaproate, hexamethylenediamine or caprolactam. Also provided herein are methods for using such an organism to produce adipate, 6-aminocaproate, hexamethylenediamine or caprolactam.Type: ApplicationFiled: December 16, 2013Publication date: November 6, 2014Applicant: Genomatica, Inc.Inventors: Anthony P. Burgard, Robin E. Osterhout, Stephen J. Van Dien, Cara Ann Tracewell, Priti Pharkya, Stefan Andrae
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Publication number: 20140323689Abstract: Process for the preparation of a viral protease inhibitor and intermediates useful in its preparation.Type: ApplicationFiled: April 21, 2014Publication date: October 30, 2014Applicant: DIPHARMA FRANCIS S.r.I.Inventors: Anna IULIANO, Varsha Ravindra JUMDE, Emanuele ATTOLINO, Maurizio TADDEI
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Publication number: 20140308716Abstract: Provided is a novel method for preparing metabolites of atorvastatin using bacterial cytochrome P450, and a composition therefor, and more particularly, a composition for preparing 2-hydroxylated product of 4-hydroxylated product from atorvastatin including bacterial cytochrome P 450 BM3 (CYP102A1), CYP102A1 mutants, and chimeras derived from the CYP102A1 mutants, a kit therefor, and a method for preparing thereof.Type: ApplicationFiled: September 27, 2012Publication date: October 16, 2014Inventors: Chul-Ho Yun, Dong-Hyun Kim, Ji-Yeon Kang, Sun-Ha Park, Sang-Hoon Ryu
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Patent number: 8852899Abstract: Embodiments of the invention relate to the enzymatic conversion of bioderived feedstocks to commercially valuable chemicals. The enzymatic conversions of the embodiments of the invention offer the potential for lower cost routes to these value-added chemicals. Some of the chemicals that are useful include nylon intermediates such as caprolactam, adipic acid, 1,6-hexamethylene diamine; butanediols such as 1,4-butanediol, 1,3-butanediol, and 2,3-butanediol; butanols such as 1-butanol, and 2-butanol; succinic acid, butadiene, isoprene, and 3-hydroxypropanoic acid.Type: GrantFiled: June 15, 2012Date of Patent: October 7, 2014Assignee: INVISTA North America S.a.r.l.Inventors: Paul S. Pearlman, Changlin Chen, Adriana L. Botes
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Publication number: 20140242644Abstract: Process for the enzymatic synthesis of the compound of formula (I): Application in the synthesis of ivabradine and addition salts thereof with a pharmaceutically acceptable acid.Type: ApplicationFiled: February 26, 2014Publication date: August 28, 2014Applicant: LES LABORATOIRES SERVIERInventors: Sandrine Pedragosa-Moreau, François Lefoulon
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Publication number: 20140242645Abstract: The present invention is directed to a process for preparing a compound of formula I-11 through multiple-step reactions.Type: ApplicationFiled: October 22, 2012Publication date: August 28, 2014Inventors: John Y.L. Chung, Kevin Campos, Edward Cleator, Robert F. Dunn, Andrew Gibson, R Scott Hoerrner, Stephen Keen, Dave Lieberman, Zhuqing Liu, Joseph Lynch, Kevin M. Maloney, Feng Xu, Nobuyoshi Yasuda, Naoki Yoshikawa, Yong-Li Zhong
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Publication number: 20140220477Abstract: The present invention relates generally to substrates for making polymers and methods for making polymers. The present invention also relates generally to polymers and devices comprising the same.Type: ApplicationFiled: February 5, 2014Publication date: August 7, 2014Applicant: Avertica, Inc.Inventor: Nicholas Brendan Duck
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Patent number: 8785162Abstract: The invention provides for aminotransferase and oxidoreductase polypeptides and nucleic acids encoding such polypeptides. Also provided are methods of using such aminotransferase and oxidoreductase nucleic acids and polypeptides.Type: GrantFiled: December 31, 2008Date of Patent: July 22, 2014Assignee: Cargill, IncorporatedInventors: Mervyn L. De Souza, Paula M. Hicks, Sherry R. Kollmann, Jose Laplaza, Joshua M. Lundorff, Sara C. McFarlan, Erin Marasco, Wei Niu, Fernando A. Sanchez-Riera, Christopher Solheid, David P. Weiner, Peter Luginbuhl, Analia Bueno, Joslin Cuenca