Nitrogen As Only Ring Hetero Atom Patents (Class 435/121)
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Publication number: 20130045513Abstract: A process for the enzymatic reduction of an enoate (1) wherein the C?C bond of the enoate (1) is stereoselectively hydrogenated in the presence of an enoate-reductase and an oxidizable co-substrate (2) in a system which is free of NAD(P)H, a. b. in which c. A is a ketone radical (—CRO), an aldehyde radical (—CHO), a carboxyl radical (—COOR), with R?H or optionally substituted C1-C6-alkyl radical, d. R1, R2 and R3 are independently of one another H, —O—C1-C6-alkyl, —O—W with W=a hydroxyl protecting group, C1-C6-alkyl, which can be substituted, C2-C6-alkenyl, carboxyl, or an optionally substituted carbo- or heterocyclic, aromatic or nonaromatic radical, or one of R1, R2 and R3 is a —OH radical, or R1 is linked to R3 so as to become part of a 4-8-membered cycle, or R1 is linked to R so as to become part of a 4-8-membered cycle, with the proviso that R1, R2 and R3 may not be identical.Type: ApplicationFiled: October 23, 2012Publication date: February 21, 2013Applicant: BASF SEInventors: Stephan Maurer, Bernhard Hauer, Melanie Bonnekessel, Kurt Faber, Clemens Stückler
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Patent number: 8377680Abstract: The invention provides a non-naturally occurring microbial organism having a 6-aminocaproic acid, caprolactam, hexametheylenediamine or levulinic acid pathway. The microbial organism contains at least one exogenous nucleic acid encoding an enzyme in the respective 6-aminocaproic acid, caprolactam, hexametheylenediamine or levulinic acid pathway. The invention additionally provides a method for producing 6-aminocaproic acid, caprolactam, hexametheylenediamine or levulinic acid. The method can include culturing a 6-aminocaproic acid, caprolactam or hexametheylenediamine producing microbial organism, where the microbial organism expresses at least one exogenous nucleic acid encoding a 6-aminocaproic acid, caprolactam, hexametheylenediamine or levulinic acid pathway enzyme in a sufficient amount to produce the respective product, under conditions and for a sufficient period of time to produce 6-aminocaproic acid, caprolactam, hexametheylenediamine or levulinic acid.Type: GrantFiled: May 7, 2010Date of Patent: February 19, 2013Assignee: Genomatica, Inc.Inventors: Mark J. Burk, Anthony P. Burgard, Robin E. Osterhout, Priti Pharkya
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Patent number: 8377660Abstract: A method for producing an optically active compound includes reacting a nucleophile with a mixture of R- and S-stereoisomers of an azolide substrate by enzyme-catalyzed kinetic resolution so as to produce the optically active compound, wherein the azolide substrate contains an azole group used as a leaving group and an acyl group directly bonded to a nitrogen atom of the azole group.Type: GrantFiled: March 25, 2010Date of Patent: February 19, 2013Assignee: Chang Gung UniversityInventors: Shau-Wei Tsai, Pei-Yun Wang
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Patent number: 8361759Abstract: The present invention provides a scalable process for producing a concentrate containing a mass of a farnesylated dibenzodiazepinone by fermenting in an aqueous culture medium a strain of a microorganism that is capable of producing the farnesylated dibenzodiazepinone, upon completion of fermentation harvesting the fermentation broth and extracting the fermentation broth to provide an extract, and thereafter treating the extract to form the concentrate. The concentrate so produced may be utilized in downstream processes for producing pharmaceutical compounds. A strain of a Micromonospora species capable of producing a farnesylated dibenzodiazepinone at a high yield rate is provided, together with culture media for culturing microorganisms, and fermentation conditions for production of the farnesylated dibenzodiazepinone of the concentrate.Type: GrantFiled: April 21, 2010Date of Patent: January 29, 2013Assignee: Thallion Pharmaceuticals Inc.Inventors: Faustinus Yeboah, Mahmood Piraee
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Publication number: 20130018051Abstract: Bridged bicyclic heteroaryl substituted triazoles and pharmaceutical compositions containing the compounds are disclosed as being useful in inhibiting the activity of the receptor protein tyrosine kinase Axl. Methods of using the compounds in treating diseases or conditions associated with Axl activity are also disclosed.Type: ApplicationFiled: September 14, 2012Publication date: January 17, 2013Applicant: Rigel Pharmaceuticals, Inc.Inventors: Rajinder Singh, Sacha Holland, Joane Litvak, Dane Goff, Jing Zhang, Thilo J. Heckrodt
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Patent number: 8349593Abstract: Disclosed is a novel process for producing a compound represented by the general formula (1) by using a microorganism, wherein the compound is useful as a plant growth stimulant. Specifically disclosed is a process for producing a succinic acid amide compound represented by the general formula (1) [wherein Ar represents a phenyl, naphthyl or indolyl group which may have a substituent; and n represents a numeral value ranging from 1 to 4] or a salt thereof, which is characterized by culturing a microorganism of the genus Pseudomonas that is capable of synthesizing a succinic acid amide in a culture medium containing an arylalkylamine represented by the general formula (2).Type: GrantFiled: October 24, 2008Date of Patent: January 8, 2013Assignee: Sumitomo Chemical Company, LimitedInventors: Toru Kitamura, Hiroshi Soejima, Tamizi Sugiyama
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Publication number: 20120295947Abstract: The present invention relates to processes and intermediates useful in the preparation of (R)-2-(7-(4-cyclopentyl-3-(trifluoromethyl)benzyloxy)-1,2,3,4-tetrahydrocyclopenta[b]indol-3-yl)acetic acid of Formula (Ia) and salts thereof, an S1P1 receptor modulator that is useful in the treatment of S1P1 receptor-associated disorders, for example, diseases and disorders mediated by lymphocytes, transplant rejection, autoimmune diseases and disorders, inflammatory diseases and disorders (e.g., acute and chronic inflammatory conditions), cancer, and conditions characterized by an underlying defect in vascular integrity or that are associated with angiogenesis such as may be pathologic (e.g., as may occur in inflammation, tumor development and atherosclerosis).Type: ApplicationFiled: January 27, 2011Publication date: November 22, 2012Applicant: ARENA PHARMACEUTICALS, INC.Inventors: Antonio Garrido Montalban, Daniel J. Buzard, John A. DeMattei, Tawfik Gharbaoui, Stephen R. Johannsen, Ashwin M. Krishnan, Young Mi Khulman, You-An Ma, Michael John Martinelli, Suzanne Michiko Sato, Dipanjan Sengupta
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Patent number: 8309078Abstract: A polypeptide molecule able to selectively modulate uric acid conversion into S(+)- allantoin is described. A pharmaceutical composition for treating uric acid related disorders and a process to selectively modulate uric acid conversion into S(+)-allantoin are also disclosed.Type: GrantFiled: November 7, 2006Date of Patent: November 13, 2012Assignee: Università degli Studi di ParmaInventors: Riccardo Percudani, Claudia Folli, Ileana Ramazzina
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Publication number: 20120282661Abstract: The invention provides a non-naturally occurring microbial organism having a 6-aminocaproic acid, caprolactam, hexametheylenediamine or levulinic acid pathway. The microbial organism contains at least one exogenous nucleic acid encoding an enzyme in the respective 6-aminocaproic acid, caprolactam, hexametheylenediamine or levulinic acid pathway. The invention additionally provides a method for producing 6-aminocaproic acid, caprolactam, hexametheylenediamine or levulinic acid. The method can include culturing a 6-aminocaproic acid, caprolactam or hexametheylenediamine producing microbial organism, where the microbial organism expresses at least one exogenous nucleic acid encoding a 6-aminocaproic acid, caprolactam, hexametheylenediamine or levulinic acid pathway enzyme in a sufficient amount to produce the respective product, under conditions and for a sufficient period of time to produce 6-aminocaproic acid, caprolactam, hexametheylenediamine or levulinic acid.Type: ApplicationFiled: July 13, 2012Publication date: November 8, 2012Applicant: GENOMATICA, INC.Inventors: Mark J. Burk, Anthony P. Burgard, Robin E. Osterhout, Priti Pharkya
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Publication number: 20120264179Abstract: The invention provides a non-naturally occurring microbial organism having an adipate, 6-aminocaproic acid or caprolactam pathway. The microbial organism contains at least one exogenous nucleic acid encoding an enzyme in the respective adipate, 6-aminocaproic acid or caprolactam pathway. The invention additionally provides a method for producing adipate, 6-aminocaproic acid or caprolactam. The method can include culturing an adipate, 6-aminocaproic acid or caprolactam producing microbial organism, where the microbial organism expresses at least one exogenous nucleic acid encoding an adipate, 6-aminocaproic acid or caprolactam pathway enzyme in a sufficient amount to produce the respective product, under conditions and for a sufficient period of time to produce adipate, 6-aminocaproic acid or caprolactam.Type: ApplicationFiled: April 20, 2012Publication date: October 18, 2012Applicant: GENOMATICA, INC.Inventors: Anthony P. Burgard, Priti Pharkya, Robin E. Osterhout
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Patent number: 8288132Abstract: The present invention discloses a chemoenzymatic process for the preparation of an iminocyclitol corresponding to formula (I), (II), (III) or (IV), wherein: R1 and R2 are the same or different, and independently selected from the group consisting of: H, OH, hydroxymethyl, methyl, ethyl, butyl, pentyl, hexyl, octyl, isopropyl, isobutyl, 2-methylbutyl, and benzyl; R3 is selected from the group consisting of: H, hydroxymethyl, hydroxyethyl, ethyl, butyl, pentyl, hexyl, octyl, dodecyl, isobutyl, isopropyl, isopentyl, 2-methylbutyl, benzyl, and phenylethyl; n: 0 or 1; the process comprising: (i) an aldol addition catalyzed by a D-fructose-6-phosphate aldolase enzyme (FSA) and an acceptor aminoaldehyde; and (ii) an intramolecular reductive amination of the addition adduct obtained in step (i) with H2, in the presence of a metallic catalyst, optionally being carried out said step (ii) in the presence of an aldehyde of formula R3—CHO, wherein R3 is as defined above.Type: GrantFiled: August 30, 2007Date of Patent: October 16, 2012Assignees: Consejo Superior de Investigaciones Cientificas, Bioglane, S.L.N.E.Inventors: Pere Clapés Saborit, Jesús Joglar Tamargo, José Antonio Castillo Expósito, Carles Lozano Pérez
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Publication number: 20120244581Abstract: The present disclosure provides substantially enantiomerically pure heterobicyclic compounds of the following structural formulas, wherein A, M, M?, and R5 are as described herein, and to biocatalytic processes for their preparation, and to the enzymes used in those processes.Type: ApplicationFiled: March 30, 2012Publication date: September 27, 2012Applicant: CODEXIS, INC.Inventors: Benjamin Mijts, Sheela Muley, Jack Liang, Lisa M. Newman, Xiyun Zhang, James Lalonde, Michael D. Clay, Jun Zhu, John M. Gruber, Jeffrey Colbeck, John D. Munger, JR., Jagadeesh Mavinahalli, Roger Sheldon
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Publication number: 20120244138Abstract: Methods and compositions for detoxifying nitrobenzodiazepines with nitroreductase mutants.Type: ApplicationFiled: March 23, 2012Publication date: September 27, 2012Applicant: Academia SinicaInventors: Andrew H-J. Wang, Shiuan-Woei Lin Wu, Fu-Chuo Peng, Che-An Wu
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Patent number: 8273556Abstract: 4-(Indol-3-ylmethyl)-4-hydroxy-2-oxoglutarate, which is useful as an intermediate in the synthesis of monatin, may be synthesized from indole pyruvic acid and pyruvic acid (and/or oxaloacetic acid) by using a novel aldolase derived from the genus Pseudomonas, Erwinia, Flavobacterium, or Xanthomonas.Type: GrantFiled: September 30, 2011Date of Patent: September 25, 2012Assignee: Ajinomoto Co., Inc.Inventors: Masakazu Sugiyama, Kunihiko Watanabe, Shigeru Kawahara
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Publication number: 20120238538Abstract: Macrocyclic amide WH-21091 with antibacterial and antitumor activities, its analogs, preparation methods and uses thereof. The said macrocyclic amides are prepared by microbes of Xenorhabdus and Photorhabdus, or they can be prepared by other living beings through transgenic techniques. The compositions of the said macrocyclic amide and its analogs can be used as drugs and/or agricultural chemicals for treatment of microbial infections, especially for treatment of infectious diseases of Staphylococcus aureus with drug resistance. The said compositions can also be used as drugs for treatment of cancers of human beings or animals.Type: ApplicationFiled: November 30, 2010Publication date: September 20, 2012Applicant: YUXI WINHEY BIO-TECH CO., LTD.Inventors: Chongren Yang, Haizhou Li, Ping Zhao, Yingjun Zhang
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Publication number: 20120231512Abstract: The present invention relates to a method for preparing alpha-ketopimelic acid, comprising converting 2-hydroxyheptanedioic acid into alpha-ketopimelic acid, which conversion is catalysed using a biocatalyst. Further, the invention relates to a heterologous cell, comprising a nucleic acid sequence encoding an enzyme having catalytic activity in the conversion of 2-hydroxyheptanedioic acid into alpha-ketopimelic acid. Further, the invention relates to the use of a heterologous cell according to the invention in the preparation of caprolactam, diaminohexane or adipic acid.Type: ApplicationFiled: September 10, 2010Publication date: September 13, 2012Applicant: DSM IP ASSETS B.V.Inventors: Petronella Catharina Raemakers-Franken, Axel Christoph Trefzer, Linda Vermote
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Patent number: 8263371Abstract: The claimed invention relates to a process for preparing (meth)acrylates of N-hydroxyalkylated amides, in which open-chain N-hydroxyalkylated amides are esterified with (meth)acrylic acid or transesterified with at least one (meth)acrylic ester in the presence of at least one heterogeneous catalyst selected from the group consisting of an inorganic salt and an enzyme.Type: GrantFiled: March 25, 2011Date of Patent: September 11, 2012Assignee: BASF AktiengesellschaftInventors: Frank Hoefer, Dietmar Haering
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Publication number: 20120225451Abstract: The present invention relates to genetic modifications in eukaryotic host cells that have been transformed to express a xylose isomerase that confers on the host cell the ability to isomerize xylose to xylulose. These genetic modifications are aimed at improving the efficiency of xylose metabolism and include. e.g., reduction of nonspecific aldose reductase activity, increased xylulose kinase activity and increased flux of the pentose phosphate pathway. The modified host cells of the invention are suitable for the production of a wide variety of fermentation products, including ethanol, in fermentation processes in which a source of xylose or a source of xylose and glucose are used as carbon source.Type: ApplicationFiled: September 2, 2011Publication date: September 6, 2012Applicant: Technische Universiteit DelftInventors: Aaron Adriaan WINKLER, Sipko Maarten Kuyper, Wilhelmus Theodorus Antonius Maria De Laat, Johannes Pieter Van Dijken, Jacobus Thomas Pronk
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Publication number: 20120225452Abstract: A novel xylose isomerase nucleotide sequence obtained from a bovine rumen fluid metagenomic library and also provides the amino acid sequence encoded by the nucleotide sequence, and a vector and a transformant containing the nucleotide sequence. When the xylose isomerase is expressed, a host cell is endowed with the capability of converting xylose into xylulose, and the xylulose is further metabolized by the host cell. Therefore, the host cell can take the xylose as a carbon source for growth. The xylose isomerase from a new source is expressed with high activity in Saccharomyces cerevisiae and is a mesophilic enzyme with optimal temperature of 60° C.Type: ApplicationFiled: June 20, 2011Publication date: September 6, 2012Applicant: SHAN DONG UNIVERSITYInventors: Xiaoming Bao, Yu Shen, Ruilei GE
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Publication number: 20120190085Abstract: The present disclosure relates to methods of using transaminase polypeptides in the synthesis of chiral amines from prochiral ketones.Type: ApplicationFiled: June 21, 2010Publication date: July 26, 2012Applicant: CODEXIS, INC.Inventors: Gregory Hughes, Paul N. Devine, Fred J. Fleitz, Brendan T. Grau, John Limanto, Christopher Savile, Emily Mundorff
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Publication number: 20120183993Abstract: The invention relates to a process for the preparation of a fermentation product from ligno-cellulosic material, comprising the following steps: a) optionally pre-treatment b) optionally washing; c) enzymatic hydrolysis; d) fermentation; and e) optionally recovery of a fermentation product; wherein in step c) an enzyme composition is used that has a temperature optimum of 55 degrees C. or more, the hydrolysis time is 40 hours or more and the temperature is 50 degrees C. or more.Type: ApplicationFiled: October 5, 2010Publication date: July 19, 2012Applicant: DSM IP ASSETS B.V.Inventors: Johannes Petrus Smits, Elisabeth Maria Gierveld, Fop Cornelis Van Der Hor
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Publication number: 20120177722Abstract: This invention provides polypeptides having lyase activity, polynucleotides encoding these polypeptides, and meth-°ds of making and using these polynucleotides and polypeptides. In one aspect, the invention is directed to polypeptides having ammonia lyase activity, e.g., phenylalanine ammonia lyase, tyrosine ammonia lyase and/or histidine ammonia lyase activity, including thermostable and thermotolerant activity, and polynucleotides encoding these enzymes, and making and using these polynucleotides and polypeptides. The polypeptides of the invention can be used in a variety of pharmaceutical, agricultural and industrial contexts. X?NO2, Cl, Br, NH2, OH, H, alkyl at one or several o, m, and p positions R?H or alkyl.Type: ApplicationFiled: November 2, 2011Publication date: July 12, 2012Inventors: David P. Weiner, Alexander Varvak, Toby Richardson, Mircea Podar, Ellen Burke, Shaun Healey
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Patent number: 8206955Abstract: Methods and compositions that can be used to make monatin or salt thereof from glucose, tryptophan, indole-3-lactic acid, indole-3-pyruvate, and 2-hydroxy 2-(indol-3-ylmethyl)-4-keto glutaric acid, are provided. Methods are also disclosed for producing the indole-3-pyruvate and 2-hydroxy 2-(indol-3-ylmethyl)-4-keto glutaric acid intermediates. Compositions provided include nucleic acid molecules, polypeptides, chemical structures, and cells. Methods include in vitro and in vivo processes, and the in vitro methods include chemical reactions.Type: GrantFiled: October 21, 2004Date of Patent: June 26, 2012Assignee: Cargill, IncorporatedInventors: Sara C. McFarlan, Paula M. Hicks, Mary Jo Zidwick, Fernando A. Sanchez-Riera, Douglas C. Cameron, Mervyn L. deSouza, John Rosazza, Steven J. Gort, Timothy W. Abraham
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Publication number: 20120156737Abstract: The invention relates to a method for preparing alpha-ketopimelic acid, comprising converting alpha-ketoglutaric acid into alpha-ketoadipic acid and converting alpha-ketoadipic acid into alpha-ketopimelic acid, wherein at least one of these conversions is carried out using a heterologous biocatalyst. The invention further relates to a heterologous cell, comprising one or more heterologous nucleic acid sequences encoding one or more heterologous enzymes capable of catalysing at least one reaction step in the preparation of alpha-ketopimelic acid from alpha-ketoglutaric acid.Type: ApplicationFiled: March 11, 2010Publication date: June 21, 2012Applicant: DSM IP ASSETS B.V.Inventors: Petronella Catharina Raemakers-Franken, Martin Schurmann, Axel Christoph Trefzer, Stefaan Marie Andre De Wildeman
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Patent number: 8202711Abstract: The present invention relates to a process for producing efficiently glutamic acid derivatives (including salts thereof) such as monatin by converting a substituted ?-keto acid of formula (1) into a glutamic acid derivative of formula (2) in the presence of an enzyme catalyzing conversion of the same.Type: GrantFiled: April 27, 2010Date of Patent: June 19, 2012Assignee: Ajinomoto Co., Inc.Inventors: Masakazu Sugiyama, Kunihiko Watanabe, Nao Funakoshi, Yusuke Amino, Shigeru Kawahara, Tadashi Takemoto
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Patent number: 8192976Abstract: Aspects of the invention relate to methods for the production of difunctional alkanes in host cells. In particular, aspects of the invention describe components of genes associated with the difunctional alkane production from carbohydrate feedstocks in host cells. More specifically, aspects of the invention describe metabolic pathways for the production of adipic acid, aminocaproic acid, caprolactam, and hexamethylenediamine via 2-ketopimelic acid.Type: GrantFiled: December 14, 2009Date of Patent: June 5, 2012Assignee: Celexion, LLCInventors: Brian M. Baynes, John Michael Geremia
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Patent number: 8183019Abstract: A process is disclosed for preparing (3R,4R)-3-hydroxy-4-hydroxymethylpyrrolidine, the compound of formula (I), or (3S,4S)-3-hydroxy-4-hydroxymethylpyrrolidine, the compound of formula (Ia) involving, as a key step, the enzyme-catalysed enantioselective hydrolysis of a racemic 3,4-trans-disubstituted pyrrolidinone compound of formula (II).Type: GrantFiled: June 3, 2005Date of Patent: May 22, 2012Assignee: Industrial Research LimitedInventors: Dirk Henning Lenz, Jennifer Mary Mason, Keith Clinch, Gary Brian Evans, Peter Charles Tyler
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Publication number: 20120123134Abstract: The present invention relates to a novel manufacturing process and novel intermediates useful in the synthesis of pharmaceutically active compounds of general formula I used for treatment of epilepsy, neuropathic pain, anxiety and social phobia. The invention describes preparation of enantiomerically pure (S)-Pregabalin from chiral pyrrolidin-2-one of formula IV.Type: ApplicationFiled: November 1, 2010Publication date: May 17, 2012Applicant: Drug Process Licensing Associates LLCInventor: Milan Soukup
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Patent number: 8153405Abstract: Products and methods for the in vivo production of monatin sweetener are provided. The products include microorganisms that are genetically modified to secrete or to improve secretion of monatin; microorganisms that are genetically modified to produce monatin; and microorganisms that are genetically modified to both secrete or improve secretion of monatin and produce monatin. The methods include producing monatin in such genetically engineered microorganisms.Type: GrantFiled: April 20, 2006Date of Patent: April 10, 2012Assignee: Cargill, IncorporatedInventors: James C. Anderson, Mervyn L. DeSouza, Paula M. Hicks, Sherry R. Kollmann, Jose Laplaza
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Publication number: 20120070867Abstract: A process for the enzymatic reduction of an enoate (1) wherein the C?C bond of the enoate (1) is stereoselectively hydrogenated in the presence of an enoate-reductase and an oxidizable co-substrate (2) in a system which is free of NAD(P)H, a. b. in which c. A is a ketone radical (—CRO), an aldehyde radical (—CHO), a carboxyl radical (—COOR), with R?H or optionally substituted C1-C6-alkyl radical, d. R1, R2 and R3 are independently of one another H, —O-C1-C6-alkyl , —O—W with W=a hydroxyl protecting group, C1-C6-alkyl, which can be substituted, C2-C6-alkenyl, carboxyl, or an optionally substituted carbo- or heterocyclic, aromatic or nonaromatic radical, or one of R1, R2 and R3 is a —OH radical, or R1 is linked to R3 so as to become part of a 4-8-membered cycle, or R1 is linked to R so as to become part of a 4-8-membered cycle, with the proviso that R1, R2 and R3 may not be identical.Type: ApplicationFiled: May 31, 2010Publication date: March 22, 2012Applicant: BASF SEInventors: Steffen Maurer, Bemhard Hauer, Melanie Bonnekesse, Kurt Faber, Clemens Stückler
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Patent number: 8133704Abstract: Aspects of the invention relate to methods for the production of difunctional alkanes in host cells. In particular, aspects of the invention describe components of genes associated with the difunctional alkane production from carbohydrate feedstocks in host cells. More specifically, aspects of the invention describe metabolic pathways for the production of adipic acid, aminocaproic acid, caprolactam, and hexamethylenediamine via 2-ketopimelic acid.Type: GrantFiled: March 18, 2011Date of Patent: March 13, 2012Assignee: Celexion, LLCInventors: Brian M. Baynes, John Michael Geremia
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Patent number: 8119375Abstract: An improved method for preparing ramipril is disclosed, and also an intermediate for use in the method.Type: GrantFiled: June 19, 2008Date of Patent: February 21, 2012Assignee: Sanofi-Aventis Deutschland GmbHInventors: Holger Berk, Frank Zocher, Hans-Wolfram Flemming, Rainer Gauler, Rudolf Lehnert, Wolfgang Laux
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Publication number: 20120040416Abstract: 4-(Indol-3-ylmethyl)-4-hydroxy-2-oxoglutarate, which is useful as an intermediate in the synthesis of monatin, may be synthesized from indole pyruvic acid and pyruvic acid (and/or oxaloacetic acid) by using a novel aldolase derived from the genus Pseudomonas, Erwinia, Flavobacterium, or Xanthomonas.Type: ApplicationFiled: September 30, 2011Publication date: February 16, 2012Applicant: Ajinomoto Co., Inc.Inventors: Masakazu SUGIYAMA, Kunihiko Watanabe, Shigeru Kawahara
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Patent number: 8114640Abstract: A method of preparing macrocycles using solid support chemistry and thioesterases is disclosed. Also disclosed are novel macrocycles.Type: GrantFiled: October 30, 2008Date of Patent: February 14, 2012Assignee: The Regents of the University of MichiganInventors: David H. Sherman, Wolfgang Seufert, Zachary Q. Beck
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Publication number: 20120034660Abstract: A process for producing optically active succinimide derivatives as key intermediates of (3R)-2?-(4-bromo-2-fluorobenzyl)spiro{pyrrolidine-3,4?(1H)-pyrrolo[1,2-a]pyrazine}-1?,2,3?,5(2?H)-tetraone, which comprises the following reaction steps.Type: ApplicationFiled: February 5, 2010Publication date: February 9, 2012Applicants: KYOWA HAKKO BIO CO., LTD., DAINIPPON SUMITOMO PHARMA CO., LTD.Inventors: Masaji Kasai, Shinji Kita, Tadashi Ogawa, Hideaki Tokai
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Publication number: 20120028309Abstract: The present invention provides a method for in vitro producing an indole derivative in a one-pot reaction. The method for producing a rhamnosylated indolocarbazole compound includes the steps of transforming a plasmid carrying a gene encoding N-glycosyltransferase into a bacterial strain; expressing the gene encoding N-glycosyltransferase in the bacterial strain; lysing the bacterial strain to obtain a crude enzyme extract; and adding TDP-glucose, an indolocarbazole aglycone and a metal ion in the crude enzyme extract for performing an enzymatic reaction to form the rhamnosylated indolocarbazole compound. Alternatively, the method for producing an indole-3-carboxaldehyde analog includes the steps of transforming a plasmid carrying a gene encoding NokA of Nocardiopsis sp.Type: ApplicationFiled: August 2, 2010Publication date: February 2, 2012Applicant: National Chiao Tung UniversityInventor: Hsien-Tai Chiu
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Patent number: 8105807Abstract: The present invention relates to carbamoylglycine derivatives, a process for the preparation of carbamoylglycine derivatives and the use of carbamoylglycine derivatives in the preparation of enantiomerically enriched ?-amino acids. Furthermore, the present invention relates to the preparation of pharmaceutically active products such as perindopril and ramipril using the novel carbamoylglycine derivatives.Type: GrantFiled: October 2, 2008Date of Patent: January 31, 2012Assignee: DSM IP Assets B.V.Inventors: Georgios Sarakinos, Ben De Lange
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Publication number: 20120021469Abstract: The disclosure relates to engineered amidase polypeptides and processes of using the polypeptides for chiral resolution of amino acid amide compounds. The disclosure further relates to the polynucleotides that encode the engineered amidase polypeptides and related vectors, host cells, and methods for making the engineered amidase polypeptides.Type: ApplicationFiled: March 26, 2010Publication date: January 26, 2012Applicant: CODEXIS, INC.Inventors: Owen Gooding, Robert J. Jones, Gjalt Huisman, Jie Yang, Louis Clark
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Publication number: 20120009634Abstract: This invention relates to polypeptides having aldolase activity, including pyruvate activity such as, without limitation, HMG and/or KHG aldolase activity, polynucleotides encoding these polypeptides, and methods of making and using these polynucleotides and polypeptides. In some embodiments, the invention is directed to polypeptides having aldolase activity, including pyruvate activity such as, without limitation, HMG and/or KHG aldolase activity, including thermostable and thermotolerant activity, and polynucleotides encoding these enzymes, and making and using these polynucleotides and polypeptides. The polypeptides in accordance with the invention can be used in a variety of pharmaceutical, agricultural and industrial contexts.Type: ApplicationFiled: September 16, 2011Publication date: January 12, 2012Inventors: Ellen BURKE, Steven John Gort, Paula M. Hicks, Peter Luginbuhl, Sara C. McFarlan, Toby Richardson, Christopher Solheid, David Weiner, Lishan Zhao
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Publication number: 20110312034Abstract: The invention relates to novel molecules and libraries thereof as well as methods for their production. Methods of producing the novel molecules include the cleaving of starting molecules into molecular subunits and the assembly of the subunits into novel recombined molecules.Type: ApplicationFiled: March 2, 2011Publication date: December 22, 2011Inventor: Dennis M. Brown
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Publication number: 20110306649Abstract: Prodrugs of ion channeling modulating compounds, including, for example, prodrugs of the ion channel modulating compound of the following formula: are described herein, as well as methods of making and using such prodrugs and pharmaceutical compositions containing such prodrugs.Type: ApplicationFiled: June 2, 2011Publication date: December 15, 2011Applicant: Cardiome Pharma Corp.Inventors: Doug Ta Hung Chou, Adewale Eniade, Bertrand M. C. Plouvier
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Patent number: 8076108Abstract: Monatin and certain stereoisomers of monatin, such as R,R monatin and S,R monatin, as well as salts thereof, are produced using polypeptides and biosynthetic pathways. These polypeptides and biosynthetic pathways are also useful in the production of R-2-hydroxy-2-(indoly-3-ylmethyl)-4-keto glutaric acid, an intermediate that is formed in certain monatin synthesis pathways, including some biosynthetic pathways.Type: GrantFiled: March 6, 2007Date of Patent: December 13, 2011Assignee: Cargill, IncorporatedInventors: Brian J. Brazeau, Mervyn L. De Souza, Steven J. Gort, Paula M. Hicks, Sherry R. Kollmann, Jose M. Laplaza, Sara C. McFarlan, Fernando A. Sanchez-Riera, Christopher Solheid
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Patent number: 8076107Abstract: Methods and materials for the production of the high intensity sweetener, monatin, in stereoisomerically-pure or stereoisomerically-enriched form are disclosed. For example, methods using stereoisoselective hydrolysis and separation of a monatin-derived lactone ester are disclosed.Type: GrantFiled: October 1, 2007Date of Patent: December 13, 2011Assignee: Cargill, IncorporatedInventors: Subash Buddoo, Amanda Louise Rousseau, Dean Brady, James Lalonde, Yiming Yao, Yifong Wang
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Publication number: 20110300282Abstract: Methods for preferentially hydrolyzing one stereoisomer of an isoxazoline diester over another, as well as an enzyme for facilitating the preferential hydrolysis are provided. Also provided are methods for providing mixtures of (RR) and (RS) monatin as well as (SS) and (SR) monatin, which methods can include the step of stereoselectively hydrolyzing an isoxazoline diester.Type: ApplicationFiled: August 18, 2011Publication date: December 8, 2011Applicant: CARGILL, INCORPORATEDInventors: Dean Brady, Lucia H. Steenkamp, Amanda Louise Rousseau, Subash Buddoo, Paul A. Steenkamp
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Publication number: 20110300589Abstract: The present invention relates to bacterial strains, capable of utilizing glycerol as a carbon source for the fermentative production of succinic acid, wherein said strains are genetically modified so that they comprise a deregulation of their endogenous pyruvate-formate-lyase enzyme activity, as well as to methods of producing organic acids, in particular succinic acid, by making use of such microorganism. The present invention also relates to the downstream processing of the produced organic acids by cation exchange chromatography.Type: ApplicationFiled: February 12, 2010Publication date: December 8, 2011Applicant: BASF SEInventors: Hartwig Schroder, Stefan Haefner, Gregory Von Abendroth, Rajan Hollmann, Aline Raddatz, Hansgeorg Ernst, Hans Gurski
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Publication number: 20110294170Abstract: The present invention provides recombinant nucleic acid constructs comprising a xylose isomerase polynucleotide, a recombinant fungal host cell comprising a recombinant xylose isomerase polynucleotide, and related methods.Type: ApplicationFiled: May 27, 2011Publication date: December 1, 2011Applicant: CODEXIS, INC.Inventors: Ezhilkani Subbian, Xiyun Zhang, Benjamin Mijts, Catherine M. Cho
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Publication number: 20110287494Abstract: Novel enzymes that stereoselectively reduce imine derivatives were isolated and purified, and polynucleotides encoding the enzymes were cloned. Optically active amine derivatives were produced by acting on imine derivatives with a culture of microorganisms having the ability to stereoselectively reduce the compounds, microbial cells or processed products thereof, and/or imine reductases thereof, followed by collecting the generated optically active amine derivatives. The present invention enables, for example, production of an optically active compound represented by formula (IV): (wherein R group represents an alkyl group having one to three carbon atoms; and n represents an integer of 1 to 4).Type: ApplicationFiled: September 1, 2009Publication date: November 24, 2011Applicant: Daicel Chemical Industries LtdInventors: Toru Nagasawa, Toyokazu Yoshida, Koichi Ishida, Hiroaki Yamamoto, Norihiro Kimoto
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Patent number: 8058034Abstract: A D-aminotransferase can be modified so as to efficiently produce (2R,4R)-monatin having high sweetness intensity from 4-(indol-3-ylmethyl)-4-hydroxy-2-oxoglutaric acid by substituting an amino acid at least at one of positions (positions 100, 180 to 183, 243 and 244) involved in efficiently producing the (2R,4R)-monatin in an amino acid sequence of a wild-type D-aminotransferase represented in SEQ ID NO:2.Type: GrantFiled: April 12, 2010Date of Patent: November 15, 2011Assignee: Ajinomoto Co., Inc.Inventors: Masakazu Sugiyama, Kunihiko Watanabe, Tatsuki Kashiwagi, Ei-ichiro Suzuki
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Patent number: 8058038Abstract: 4-(Indol-3-ylmethyl)-4-hydroxy-2-oxoglutarate, which is useful as an intermediate in the synthesis of monatin, may be synthesized from indole pyruvic acid and pyruvic acid (and/or oxalacetic acid) by using a novel aldolase derived from the genus Pseudomonas, Erwinia, Flavobacterium, or Xanthomonas.Type: GrantFiled: December 19, 2007Date of Patent: November 15, 2011Assignee: Ajinomoto Co., Inc.Inventors: Masakazu Sugiyama, Kunihiko Watanabe, Shigeru Kawahara
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Publication number: 20110269180Abstract: The present invention relates to the use of nucleic acid molecules coding for a bacterial xylose isomerase (XI), preferably coming from Clostridium phytofermentans, for reaction/metabolization, particularly fermentation, of recombinant microorganisms of biomaterial containing xylose, and particularly for the production of bioalcohols, particularly bioethanol, by means of xylose fermenting yeasts. The present invention further relates to cells, particularly eukaryotic cells, which are transformed utilizing a nucleic acid expression construct which codes for a xylose isomerase, wherein the expression of the nucleic acid expression construct imparts to the cells the capability to directly isomerize xylose into xylulose. Said cells are preferably utilized for reaction/metabolization, particularly fermentation, of biomaterial containing xylose, and particularly for the production of bioalcohols, particularly bioethanol.Type: ApplicationFiled: July 1, 2009Publication date: November 3, 2011Inventors: Dawid Brat, Eckhard Boles, Marco Keller, Beate Wiedemann