Oxygen As Only Ring Hetero Atom Patents (Class 435/123)
-
Patent number: 5369021Abstract: A microorganism for selective production of a specific compound of avermectin having one or more of the following properties:specific accumulation of avermectin compound "a",effective incorporation of isoleucine or its keto acid (3-methyl-2-oxovaleric acid) into avermectin molecule, andmarkedly suppressed incorporation of valine or its keto acid (2-oxoisovaleric acid) into avermectin molecule.Type: GrantFiled: December 30, 1992Date of Patent: November 29, 1994Assignee: The Kitasato InstituteInventors: Omura Satoshi, Ikeda Haruo
-
Patent number: 5358860Abstract: A method of converting olefins to chiral epoxides comprises combining an asymmetric aliphatic or aryl alkene substrate with a buffered chloroperoxidase solution to form a stabilized reaction mixture, and gradually adding hydrogen peroxide as a substrate oxidant, such that the chloroperoxidase catalyzes the conversion of the substrate to the corresponding epoxide in enantiomeric excess. The products of the invention are alkyl and aryl non-primary epoxides. The resulting preparations are enantiomerically pure, and may greatly enhance large-scale synthesis of stereoisomer products such as pharmaceuticals and pesticides.Type: GrantFiled: April 5, 1993Date of Patent: October 25, 1994Assignee: The Board of Trustees of the University of IllinoisInventors: Lowell P. Hager, Eric J. Allain
-
Patent number: 5334732Abstract: A process for oxidizing cephalomannine and related taxanes in the presence of taxol is described. The process involves the use of ozone which is able to oxidize cephalomannine and easily oxidizable taxanes while leaving taxol essentially unchanged. Taxol can then be separated from the oxidized cephalomannine and other oxidized taxanes using conventional procedures. A process for oxidizing taxanes is also provided.Type: GrantFiled: September 10, 1993Date of Patent: August 2, 1994Assignee: Hauser Chemical Research, Inc.Inventors: Christopher K. Murray, Timothy D. Ziebarth, Jeffrey T. Beckvermit
-
Patent number: 5322779Abstract: Taxol is produced from a taxol-producing microorganism Taxomyces andreanae BS 279.92. Radioactive taxol products and methods for use of the radioactive labelled taxol are described.Type: GrantFiled: November 4, 1992Date of Patent: June 21, 1994Assignee: The Research and Development Institute, Inc. at Montana State UniversityInventors: Gary A. Strobel, Andrea A. Stierle, Donald B. Stierle
-
Patent number: 5322782Abstract: Optically active .beta.-halolactic acids can be produced by contacting an .alpha.,.beta.-dihalopropionic acid with 2-halo acid dehalogenase. When the pH of the reaction system is above 9, this process gives optically active glycidic acid. Treatment of the optically active .beta.-halolactic acid thus obtained with an alkali also gives optically active glycidic acid.Type: GrantFiled: October 19, 1992Date of Patent: June 21, 1994Assignee: Unitika Ltd.Inventors: Hiroshi Nakajima, Masaaki Onda, Ryoichi Tsurutani, Kenzo Motosugi
-
Patent number: 5312740Abstract: A callus and suspension culture cells containing taxol as an effective component, and a process for producing taxol therefrom, are provided. The callus and suspension culture cells containing taxol in a high content can be induced and proliferated by culturing a tissue of a plant belonging to the genus Taxus, particularly its female gametophyte. The process for cell culture is advantageously carried out in the presence of a particular combination of plant growth regulators known per se in a growth medium. A callus or suspension culture cells containing at least taxol as an effective component can be stably provided, making it possible to effectively produce taxol.Type: GrantFiled: October 9, 1992Date of Patent: May 17, 1994Assignee: Nippon Steel CorporationInventors: Koji Saito, Hiroaki Ohashi, Masaaki Hibi, Makoto Tahara
-
Patent number: 5310672Abstract: The present invention provides methods of inducing somatic embryogenesis in tissue cultures derived from varieties of Taxus. In addition, the present invention provides methods of obtaining taxol, or precursors thereof, using in vitro propagated somatic embryo tissues.Type: GrantFiled: August 5, 1993Date of Patent: May 10, 1994Assignee: Union Camp CorporationInventors: Steven R. Wann, William R. Goldner
-
Patent number: 5306625Abstract: A microbiological process for terminal hydroxylation of ethyl groups on aromatic 5- or 6-member ring heterocycles. The hydroxylation is performed with microorganisms which:(a) contain the genes of a Pseudomonas OCT plasmid which form an active alkane monooxygenase, and(b) form no active chromosomal or plasmid-coded alcohol dehydrogenase.Type: GrantFiled: August 3, 1993Date of Patent: April 26, 1994Assignee: Lonza Ltd.Inventors: Andreas Kiener, Thomas Zimmermann
-
Patent number: 5292647Abstract: A new microorganism, Streptomyces avermitilis subspecies niger NRRL 21005, and processes using the microorganism for producing known avermectins are provided.Type: GrantFiled: November 30, 1992Date of Patent: March 8, 1994Assignee: Eli Lilly and CompanyInventors: Rosanne Bonjouklian, Otis W. Godfrey, Tim A. Smitka, Raymond C. Yao
-
Patent number: 5279949Abstract: A process for the separation of taxanes, particularly taxol, from ornamental yew tissue is described. The process involves a specific solvent mixture of ethanol and water which is between 50 to 80 percent by volume ethanol and decolorizing of the resulting extract using activated carbon containing the crude taxanes. The taxanes are separated from the crude extract by a normal phase chromatographic step which preferably is through vacuum or medium pressure column chromatographic separation, using inexpensive silica gel as an absorbent.Type: GrantFiled: December 7, 1992Date of Patent: January 18, 1994Assignee: Board of Trustees operating Michigan State UniversityInventor: Muraleedharan G. Nair
-
Patent number: 5279953Abstract: Method for improved in vivo production of taxanes by inoculation of tissue of the genus Taxus with virulent or avirulent strains of Agrobacterium. In the preferred embodiment, virulent Agrobacterium is the inoculant which induces gall formation at all stages of the plant, from saplings to mature trees. Incomplete harvesting of galls permits regeneration on the trees, resulting in a renewable resource. Unexpectedly, the inoculant tissue (both virulent and avirulent) produces approximately twice the concentration of taxanes per unit tissue weight as compared to normal tissue. In addition, the virulent strain induced gall biomass is 2-3 times greater than normal tissue per unit growth time, while avirulent inoculants show approximately the same growth as normal. The net yield increase is 2-6 times normal, (4-6 for galls), is renewable, does not require special growth hormones or media, does not result in sacrificing the tree, and can be started at the sapling stage.Type: GrantFiled: June 24, 1992Date of Patent: January 18, 1994Assignee: ESCA Genetics CorporationInventor: Roy W. Stahlhut
-
Patent number: 5278068Abstract: An organism of the Cytospora species produces substituted hexahydrobenzopyran compounds of the Formulas A, B, or C which are useful as angiotensin II antagonists: ##STR1##Type: GrantFiled: October 16, 1992Date of Patent: January 11, 1994Assignee: Merck & Co., Inc.Inventor: Gerald F. Bills
-
Patent number: 5264362Abstract: A microbiological process for the production of 6-hydroxynicotinic acid starting from 3-cyanopyridine. For this process, new microorganisms are used, which are capable of growing with 3-cyanopyridine as the sole carbon, nitrogen and energy source and of biotransforming it as substrate in 6-hydroxynicotinic acid.Type: GrantFiled: October 14, 1992Date of Patent: November 23, 1993Assignee: Lonza Ltd.Inventor: Andreas Kiener
-
Patent number: 5248601Abstract: A biotechnological process for preparing L(-)-carnitine chloride, having the formula ##STR1## comprising: (a) reacting a racemic ester of (R,S)-3,4-epoxybutyric acid having the formula ##STR2## wherein R is an alkyl group having from 1 to 10 carbons or a benzyl group, with an enzyme capable of selectively hydrolyzing enantiomer S(-);(b) separating the enantiomer S(-) from non-reacted ester which is present in predominantly the R(+) form;(c) reacting the non-reacted ester obtained in step (b) with an enzyme capable of quantitatively hydrolyzing the R(+) form to obtain thereby a salt of 3,4-epoxybutyric acid in the R(+) form having the formula ##STR3## wherein X is Na, K, or Li; (d) reacting the salt obtained in step (c) with a molar excess of trimethylamine; and(e) treating the reaction product of step (d) with HCl to remove excess trimethylamine and to obtain thereby the L(-)-carnitine chloride of formula (I).Type: GrantFiled: October 9, 1990Date of Patent: September 28, 1993Inventors: Franco Francalanci, Marco Ricci, Pietro Cesti, Carlo Venturello
-
Patent number: 5244803Abstract: There is disclosed a process for preparing optically active 3-phenylglycidic acid ester compound, which comprises permitting a culture broth, cells or treated cells of a microorganism having an ability of stereoselectively hydrolyzing a (2R, 3S)-3-phenylglycidic acid ester compound to act on a racemic 3-phenylglycidic acid ester compound which may also have a substituent on the phenyl group, thereby hydrolyzing the (2R, 3S) optically active isomer and separating and collecting the (2S, 3R) antipode from the reaction mixture.Type: GrantFiled: September 7, 1990Date of Patent: September 14, 1993Assignee: Tanabe Seiyaku Co., Ltd.Inventors: Takao Mori, Toshiyuki Furutani, Akio Nakao, Atsuhiko Tsujimura, Takeji Shibatani
-
Patent number: 5242816Abstract: A microbiological process for the terminal oxidation of alkyl groups in 5- or 6-ring heterocycles which are substituted with at least one alkyl group with more than 2 carbon atoms to carboxylic acids.Type: GrantFiled: July 3, 1991Date of Patent: September 7, 1993Assignee: Lonza Ltd.Inventor: Andreas Kiener
-
Patent number: 5238828Abstract: An efficient microbiological or biochemical method is proposed for the preparation of an optically active 2-substituted carboxylic acid, e.g., 2-chloropropionic acid, 2-methyl butyric acid and the like, from the corresponding 2-substituted nitrile compound, e.g., 2-chloropropionitrile, 2-methyl butyronitrile and the like, in the form of a racemic body as the starting material The method comprises bringing the starting nitrile compound into contact with a microorganism, such as Pseudomonas sp. MY-1 (FERM BP-2541), Fusarium sp. MY-2 (FERM BP-2542) and the like, capable of converting the nitrile compound into the carboxylic acid in a buffered aqueous medium in which the microbial cells of the microorganism are suspended.Type: GrantFiled: September 17, 1991Date of Patent: August 24, 1993Assignee: Idemitsu Kosan Company LimitedInventor: Nobuo Murakami
-
Patent number: 5236832Abstract: A microbiological process for the oxidation of methyl groups in aromatic 5- or 6-member ring heterocycles to the corresponding carboxylic acid. The heterocycle serves as the substrate and exhibits no substituent on the carbon atoms adjacent to the methyl groups to be oxidized. The reaction of the heterocycle takes place by means of microorganisms of the genus Pseudomonas utilizing toluene, xylene, or cymene. The enzymes of the microorganisms been previously induced.Type: GrantFiled: October 30, 1991Date of Patent: August 17, 1993Assignee: Lonza, Ltd.Inventor: Andreas Kiener
-
Patent number: 5232852Abstract: Alcohols of the formula ##STR1## wherein R.sup.1 and R.sup.2 are each independently methyl or ethyl or together signify pentamethylene,formed by the enzymatic hydrolysis of a corresponding (RS) alkanoic acid ester are intermediates for Vitamin E.Type: GrantFiled: March 13, 1990Date of Patent: August 3, 1993Assignee: Hoffmann-La Roche Inc.Inventors: Richard Barner, Josepf Hubscher, Beat Wirz
-
Patent number: 5213975Abstract: This invention relates to the methods of preparing optically active epoxy alcohol which is caracterized by the steps of adding carboxylic anhydride to racemic epoxy alcohol (formula 1) in the presence of a hydrolase in an organic solvent, esterifying (-)-form of the epoxy alcohol preferentially to be epoxy ester (formula 2), separating optically active epoxy ester (formula 2) from optically active epoxy alcohol (formula 3) to yield optically active epoxy alcohol (formula 4), so that epoxy alcohol (formula 1) in high purity can be obtained easily and safely at ordinary temperature.Type: GrantFiled: October 24, 1991Date of Patent: May 25, 1993Assignee: Nitto Denko Co., Ltd.Inventors: Eiichiro Fukusaki, Hiroshi Takahama, Minako Hada, Yasuyuki Kimura, Hiroyuki Yuasa, Yutaka Nakazono, Shuji Senda, Tetsuo Omata
-
Patent number: 5213973Abstract: A microbiological process for oxidation of methyl groups in aromatic 5- or 6-ring heterocycles to the corresponding carboxylic acid. The reaction of the heterocycle takes place by microorganisms of the genus Pseudomonas, which utilize toluene, xylene or cymene. An inducer, the aromatic heterocycle, as the substrate, and a carbon source and energy source are fed in and, after the maximal product concentration is reached, the product is separated.Type: GrantFiled: July 3, 1991Date of Patent: May 25, 1993Assignee: Lonza Ltd.Inventor: Frans Hoeks
-
Patent number: 5212078Abstract: Described is a microbiological method for producing the lactone, sclareolide having the structure: ##STR1## and a diol having the chemical structure: ##STR2## using a sclareol derivative having one of the structures: ##STR3## wherein a combination of two or more of the compounds as a substrate and using one of the microorganisms:Cryptococcus albidus, ATCC 20918Bensingtonia ciliata, ATCC 20919Cryptococcus laurentii, ATCC 20920 orCryptococcus albidus, ATCC 20921.Type: GrantFiled: April 25, 1990Date of Patent: May 18, 1993Assignee: International Flavors and Fragrances Inc.Inventors: Mohamad I. Farbood, James A. Morris, Arthur E. Downey
-
Patent number: 5206155Abstract: A microorganism for selective production of a specific compound of avermectin having one or more of the following properties:specific accumulation of avermectin compound "a",effective incorporation of isoleucine or its keto acid (3-methyl-2-oxovaleric acid) into avermectin molecule, andmarkedly suppressed incorporation of valine or its keto acid (2-oxoisovaleric acid) into avermectin molecule.Type: GrantFiled: August 10, 1990Date of Patent: April 27, 1993Assignee: The Kitasato InstituteInventors: Satoshi Omura, Haruo Ikeda
-
Patent number: 5204248Abstract: Disclosed is a process for preparing 2-halogeno-3-hydroxy-3-phenylpropionic acid ester compounds represented by the formula (I): ##STR1## wherein Ring A is a phenyl group which may be substituted, R.sup.1 is an ester residue, and X is a halogen atom, which comprises permitting an enzyme having the ability of stereoselectively reducing oxo group to hydroxy group to act on 2-halogeno-3-oxo-3-phenylpropionic acid ester compounds represented by the formula (II): ##STR2## wherein Ring A, R.sup.1 and X have the same meanings as defined above.Type: GrantFiled: March 22, 1991Date of Patent: April 20, 1993Assignee: Tanabe Seiyaku Co., Ltd.Inventors: Takeji Shibatani, Takuo Nishida, Hiroaki Matsumae
-
Patent number: 5192672Abstract: A purified hydroxylase component of the soluble methane monooxygenase enzyme present in the bacterium Methylosinus trichosporium OB3b is found capable of oxidizing hydrocarbons under aerobic conditions in the presence of suitable reducing agents. The hydroxylase can be reduced by commercial reducing agents, such as sodium dithionite and photo- and electrochemical means when in the presence of electron transport components, such as methyl viologen and proflavin. The hydroxylase can also be activated by hydrogen peroxide in the absence of reducing agents and molecular oxygen and is capable of oxidizing hydrocarbons under aerobic and anaerobic conditions in this manner. The hydroxylase component can be obtained with high final specific activity when ferrous iron compounds and cysteine are included in the purification buffers used to extract the hydroxylase from bacterial cells.Type: GrantFiled: June 21, 1991Date of Patent: March 9, 1993Assignee: Amoco CorporationInventor: John D. Lipscomb
-
Patent number: 5190870Abstract: A purified hydroxylase component of the soluble methane monooxygenase enzyme present in the bacterium Methylosinus trichosporium OB3b is found capable of oxidizing hydrocarbons under aerobic conditions in the presence of suitable reducing agents. The hydroxylase can be reduced by commercial reducing agents, such as sodium dithionite and photo- and electrochemical means when in the presence of electron transport components, such as methyl viologen and proflavin. The hydroxylase component can be obtained with high final specific activity when ferrous iron compounds and cysteine are included in the purification buffers used to extract the hydroxylase from bacterial cells.Type: GrantFiled: October 8, 1990Date of Patent: March 2, 1993Assignee: Amoco CorporationInventors: John D. Lipscomb, Brian G. Fox
-
Patent number: 5177007Abstract: A process for producing optically active R-(+)-2,3-dichloro-1-propanol, which comprises cultivating an S-(-)-2,3-dichloro-1-propanol-assimilating strain belonging to the genus Alcaligenes in a culture medium containing racemate 2,3-dichloro-1-propanol, and recovering optical isomer R-(+)-2,3-dichloro-1-propanol from the culture broth and a pure culture of an S-(+)-dichloro-1-propanol-assimilating strain belonging to the genus Alcaligenes.Type: GrantFiled: December 7, 1990Date of Patent: January 5, 1993Assignee: Daiso Co., Ltd.Inventor: Naoya Kasai
-
Patent number: 5173428Abstract: This invention relates to a process for the microbiological production of compounds containing a terminal hydroxyl or epoxy group from an aliphatic substrate or a substrate with an aliphatic side chain, using microorganisms genetically engineered so that they have retained their capacity to perform the terminal oxidation of the substrate, but are no longer able to convert the resulting oxidation product further to any significant extent. Preferred substrates are n-alkanes, n-alkenes, and n-alkadienes containing 6-12 carbon atoms. Preferred micro-organisms are genetically engineered Pseudomonas oleovorans and Pseudomonas putida strains lacking an active plasmidic alkanol-dehydrogenase gene. The invention also relates to micro-organisms thus genetically engineered.Type: GrantFiled: April 1, 1991Date of Patent: December 22, 1992Assignee: Rijksuniversiteit te GroningenInventors: Bernard Witholt, Roland G. Lageveen
-
Patent number: 5155028Abstract: In the enzymatic stereoselective ester cleavages of 2-arylpropionate, the reaction rate of the hydrolyzing enzymes can be drastically increased if the vinyl ester of the 2-arylpropionate is employed as the substrate.Type: GrantFiled: June 8, 1990Date of Patent: October 13, 1992Assignee: Hoechst AktiengesellschaftInventors: Gerd Fulling, Merten Schlingmann, Reinhold Keller
-
Patent number: 5155029Abstract: Described is a microbiological method for producing the cyclic ether having the chemical structure: ##STR1## using a sclareol derivative having one of the structures: ##STR2## and using the microorganism: Cryptococcus laurentii, ATCC 20920.Type: GrantFiled: July 20, 1990Date of Patent: October 13, 1992Assignee: International Flavors & Fragrances Inc.Inventors: Mohamad I. Farbood, James A. Morris, Arthur E. Downey
-
Patent number: 5151352Abstract: A clerodane-type diterpene derivative, which has antibacterial and antitumor activities, as well as chemically equivalent tautomers thereof and a process for producing said derivative using a microorganism belonging to the genus Streptomyces.Type: GrantFiled: September 30, 1991Date of Patent: September 29, 1992Assignee: Kyowa Hakko Kogyo Co., Ltd.Inventors: Hirofumi Nakano, Shozo Kawada, Yoichi Uosaki, Yutaka Saito, Katsushige Gomi, Toshiaki Iwazaki
-
Patent number: 5147791Abstract: A method for synthesizing a polyester comprises reacting a diester and a dialcohol at approximately ambient temperature in the presence of an enzyme catalyst, preferably a lipase comprising porcine pancreatic lipase. The method is particularly adapted for enantioselective polymerization resulting in an optically active polyester. The polyesters preferably exhibit a weight average molecular weight, Mw, as measured by gel permeation chromatography of at least about 3000.Type: GrantFiled: September 17, 1991Date of Patent: September 15, 1992Assignee: University of New MexicoInventors: Cary J. Morrow, Joe S. Wallace
-
Patent number: 5122461Abstract: The invention relates to a novel method for preparing pyrocatecholic compounds from monophenolic compounds using catalysis by monophenol monooxygenase in an aqueous solution containing metal ions. The metal ions form complexes with the pyrocatecholic products. This method increases the yield of pyrocatecholic products. In particular, the method of the instant invention can be used to increase the yield of L-DOPA, a drug used in the treatment of Parkinson's disease.Type: GrantFiled: November 19, 1990Date of Patent: June 16, 1992Assignee: Industrial Technology Research InstituteInventors: Kuang-Pin Hsiung, Feng-Tsun Lee, Chung-Long Hsieh
-
Patent number: 5106868Abstract: Compounds UCF1 and derivatives thereof exhibit anti-bacterial and anti-tumor activities and may be used for the preparation of anti-bacterial and anti-tumor agents.Compounds UCF1 may be produced by fermentation of a microorganism of the genus Streptomyces capable of producing compounds UCF1, preferably Streptomyces sp. UOF1 (FERM BP-2844) and recovering the desired compounds from the cultured broth.The compounds UCF1 have the formula ##STR1## wherein R represents either ##STR2## The compounds may be oxidized to obtain derivatives of formula ##STR3## which exhibit similar pharmacological activities.Type: GrantFiled: May 7, 1991Date of Patent: April 21, 1992Assignee: Kyowa Hakko Kogyo Kabushiki KaishaInventors: Hirofumi Nakano, Mitsunobu Hara, Yutaka Saito, Yoji Ikuina, Toshimitsu Takiguchi, Masami Okabe
-
Patent number: 5104798Abstract: A microbiological process for the oxidation of methyl groups in aromatic 5- or 6-member ring heterocycles to the corresponding carboxylic acid. The heterocycle serves as the substrate and exhibits no substituent on the carbon atoms adjacent to the methyl groups to be oxidized. The reaction of the heterocycle takes place by means of microorganisms of the genus Pseudomonas utilizing toluene, xylene, or cymene. The enzymes of the microorganisms have been previously induced.Type: GrantFiled: February 5, 1991Date of Patent: April 14, 1992Assignee: Lonza Ltd.Inventor: Andreas Kiener
-
Patent number: 5102793Abstract: A microbiological process for sterospecific monoketo reduction of racemic 3-keto-bicyclic carbacyclin intermediates to the corresponding optically active 30.alpha. hydroxy compounds is provided.Type: GrantFiled: July 13, 1988Date of Patent: April 7, 1992Assignee: Schering AktiengesellschaftInventors: Karl Petzoldt, Helmut Dahl, Helmut Vorbruggen
-
Patent number: 5096924Abstract: As a new antibiotic is provided a compound, now nominated as MI43-37F11 substance, which has formula ##STR1## This MI43-37F11 substance has an antitumor activity, an activity to enhance the production of interleukin-1 in vivo in a mammalian, and an activity to activate a macrophage in vivo in a mammalian. MI43-37F11 substance may be produced by cultivation of Streptoverticillium eurocidicum MI43-37F11 strain identified as FERM BP-2783.Type: GrantFiled: May 31, 1990Date of Patent: March 17, 1992Assignee: 501 Azidan Hojin Biseibutsu Kagaku Kenkyu KaiInventors: Masaaki Ishizuka, Hiroyuki Kumagai, Tsutomu Sawa, Hiroshi Naganawa, Hironobu Iinuma, Kunio Isshiki, Masa Hamada, Kenji Maeda, Tomio Takeuchi
-
Patent number: 5093248Abstract: A new antitumor antibiotic designated herein as BU-3862T is produced by fermentation of Streptomyces hygroscopicus ATCC 53709. BU-3862T and its diacetyl and dihydro derivatives inhibit the growth of tumors in experimental animals.Type: GrantFiled: November 13, 1989Date of Patent: March 3, 1992Assignee: Bristol-Myers Squibb Co.Inventors: Koko Sugawara, Masami Hatori, Hideo Kamei, Masataka Konishi, Toshikazu Oki, Koji Tomita
-
Patent number: 5087567Abstract: An antitumor antibiotic designated BMY-42428 is produced by fermentation of Actinomadura madurae ATCC-53806. The BMY-42428 antibiotic exhibits both antimicrobial and antitumor activities.Type: GrantFiled: January 12, 1990Date of Patent: February 11, 1992Assignee: Bristol-Myers Squibb CompanyInventors: Thomas T. Dabrah, Grace A. Hesler, Sandra J. Hofstead, Kin S. Lam, Jacqueline M. Mattei, Daniel R. Schroeder, Koji Tomita
-
Patent number: 5084387Abstract: Disclosed herein is a process for preparing a novel monoester of the formula ##STR1## in which the associated novel diester ##STR2## is hydrolyzed in the presence of one or more water-soluble enzymes or microorganisms capable of selectively hydrolyzing the --O--C(O)--R.sup.1 group, wherein the treatment is carried out in a biphasic solvent system comprising an aqueous phase having the enzymes or microorganisms and an organic phase immiscible in water having the diester. Also disclosed is a process for preparing [1S-[1.alpha., 2.alpha.(Z),3.alpha.,4.alpha.]]-7-[3[[[[(1-oxoheptyl)amino]acetyl]-amino]m ethyl-7-oxabicyclo-[2.2.1]hept-2-yl]-5-heptenoic acid using this enzymatic/microbial process.Type: GrantFiled: December 18, 1990Date of Patent: January 28, 1992Assignee: E. R. Squibb & Sons, Inc.Inventors: Ramesh N. Patel, Laszlo J. Szarka, John K. Thottathil, David Kronenthal
-
Patent number: 5079152Abstract: A phosphorus-containing analog-ligand having a stereoconfiguration that substantially corresponds to the stereoconfiguration of an amide- or ester-forming transition state is used to induce production of receptor molecules whose antibody combining sites have stereospecific amide or ester synthase catalytic activity when reacted with a ligand containing (i) a carbonyl carbon atom and (ii) an amine or alcohol group that are structurally capable of forming a preselected stereoisomer of a carboxylic amide or ester.Type: GrantFiled: August 7, 1987Date of Patent: January 7, 1992Assignee: Scripps Clinic and Research FoundationInventors: Stephen Benkovic, Richard A. Lerner, Alfonso Tramontano, Andrew D. Napper
-
Patent number: 5032513Abstract: A process for the preparation of gamma and delta lactones from organic carboxylic acids or derivatives thereof is disclosed. The process comprises cultivating, under aerobic conditions, a fungus of the genus Mucor in a suitable medium containing the carboxylic acid or a derivative thereof.Type: GrantFiled: June 1, 1988Date of Patent: July 16, 1991Assignee: BASF CorporationInventors: Gregory V. Page, Robert G. Eilerman
-
Patent number: 5032512Abstract: This invention relates to a process for the microbiological production of compounds containing a terminal hydroxyl or epoxy group from an aliphatic substrate or a substrate with an aliphatic side chain, using microorganisms genetically engineered so that they have retained their capacity to perform the terminal oxidation of the substrate, but are no longer able to convert the resulting oxidation product further to any significant extent. Preferred substrates are n-alkanes, n-alkenes, and n-alkadienes containing 6-12 carbon atoms. Preferred micro-organisms are genetically engineered Pseudomonas oleovorans and Pseudomonas putida strains lacking an active plasmidic alkanol-dehydrogenase gene. The invention also relates to micro-organisms thus genetically engineered.Type: GrantFiled: January 14, 1988Date of Patent: July 16, 1991Assignee: Rijksuniversiteit te GroningenInventors: Bernard Witholt, Roland G. Lageveen
-
Patent number: 5026642Abstract: In a process for the reduction of ketones, the keto compounds are enantioselectively reduced to give secondary alcohols by means of microorganisms. Keto compounds of the formulaR.sup.3 NH--CHR.sup.1 --CO--CH.sub.2 --COOR.sup.2in which R.sup.1, R.sup.2 and R.sup.3 have the meanings defined herein are used as starting compounds.Type: GrantFiled: July 22, 1988Date of Patent: June 25, 1991Assignee: Merck Patent Gesellschaft mit beschrankter HaftungInventors: Hans-Eckart Radunz, Harry Schwartz, Martin Heinrich
-
Patent number: 5019504Abstract: Tissue of Taxus brevifolia has been successfully cultured to produce taxol, related alkaloids, and alkaloid precursors. These procedures will provide a supply of chemotherapeutic agents.Type: GrantFiled: March 23, 1989Date of Patent: May 28, 1991Assignee: The United States of America as represented by the Secretary of AgricultureInventors: Alice A. Christen, Donna M. Gibson, John Bland
-
Patent number: 5017476Abstract: A biocatalytic method for reacting organic, especially poorly water-soluble substances. Enzymes, prokariotic and eukariotic cells or combinations of enzymes and cells are immobilized in lyotropic liquid crystals (mesophases), preferably with inverse phase structure. The supply of substrate and the removal of product are performed via one or more solvent phases. Organic solvents function as solvent phases. In addition, an aqueous solvent phase can also be present. In order to produce these two and three-phase systems, three-component systems consisting of water, organic solvent and surfactant are used.Type: GrantFiled: May 8, 1989Date of Patent: May 21, 1991Assignee: Degussa AktiengesellschaftInventors: Peter Miethe, Harald Voss, Ronald Gruber
-
Patent number: 5015579Abstract: A method for producing (-)trans-2, 3-epoxysuccinic acid, which can be used as a good starting material for the sythesis of optically active compounds such as optically specific single .beta.-lactam antibiotics, characterized in that filamentous fungi capable of producing (-)trans-2,3-epoxysuccinic acid are cultured in liquid medium while either ammonia, sodium hydroxide or potassium hydroxide is added to maintain the culture medium in a pH range of 5.0 to 7.5 throughout the culturing period.Type: GrantFiled: February 10, 1987Date of Patent: May 14, 1991Assignee: Takeda Chemical Industries, Ltd.Inventors: Takamasa Yamaguchi, Ikuo Nogami
-
Patent number: 4987072Abstract: The invention relates to cyclic antibiotics of formula ##STR1## wherein the prefix R represents that the substituents on the adjacent carbon atom are in the R configuration; the prefix S represents that the substituents on the adjacent carbon atom are in the S configuration; R.sub.1 is hydrogen or hydroxy and R.sub.2 is hydroxy or .beta.-glucopyranosyloxy, and to the hydrates and salts thereof, especially pharmaceutically acceptable salts.The compounds of formula I are prepared by fermentation using the strain So ce 12 (NCIB 12134) of the species Sorangium celulosum and are effective antiobiotics.Type: GrantFiled: September 11, 1986Date of Patent: January 22, 1991Assignee: Gesellschaft fur Biotechnologische Forschung GmbHInventors: Hans Reichenbach, Gerhard Hofle, Hermann Augustiniak, Norbert Bedorf, Klaus Gerth, Herbert Irschik, Rolf Jansen, Brigitte Kunze, Dietmar Schomburg, Heinrich Steinmetz, Wolfram Trowitzsch-Kienast, Victor Wray
-
Patent number: 4985357Abstract: The present invention relates to a process for the preparation of a glyceryl mononitrate or of a mixture of glyceryl mononitrates, characterized in that it comprises a bioconversion step of nitroglycerine to glyceryl mononitrate(s) by a microorganism chosen from the group consisting of the yeasts, the fungi and protozoa, preferably a bioconversion step by fungi.Type: GrantFiled: January 18, 1989Date of Patent: January 15, 1991Assignee: Societe Nationale des Poudres et ExplosifsInventors: Eric P. Wimmer, Serge L. Lecolier, Claire A. Ducrocq, Claudine G. Servy, Maryse T. Lenfant
-
Patent number: 4970163Abstract: Described is a microbilogical method for producing the lactone, sclareolide and a diol having the chemical structure: ##STR1## using sclareol having the structure: ##STR2## as a substrate and using one of the microorganisms: Cryptococcus albidus (saito [skinner var. albidus]), ATCC 20918Bensingtonia ciliata, ATCC 20919Cryptococcus laurentii, ATCC 20920Cryptococcus albidus, ATCC 20921.Type: GrantFiled: August 28, 1989Date of Patent: November 13, 1990Assignee: International Flavors & Fragrances Inc.Inventors: Mohamad I. Farbood, James A. Morris, Arthur E. Downey