Fat; Fatty Oil; Ester-type Wax; Higher Fatty Acid (i.e., Having At Least Seven Carbon Atoms In An Unbroken Chain Bound To A Carboxyl Group); Oxidized Oil Or Fat Patents (Class 435/134)
  • Patent number: 10351871
    Abstract: The amino acid and nucleic acid sequences of a ?5-desaturase enzyme and a ?8-desaturase enzyme are disclosed. The nucleic acid sequences can be used to design recombinant DNA constructs and vectors. These vectors can then be used to transform various organisms, including for example, plants and yeast. The transformed organisms will then produce polyunsaturated fatty acids. The amino acid sequences are useful for generating enzyme-specific antibodies that are useful for identifying the desaturases.
    Type: Grant
    Filed: June 13, 2017
    Date of Patent: July 16, 2019
    Assignee: Washington State University
    Inventors: John A. Browse, James G. Wallis, Jennifer L. Watts
  • Patent number: 10337037
    Abstract: A method of producing lipids, containing the steps of: culturing a transformant in which the expression of a gene encoding the following protein (A) or (B) is enhanced, and producing long-chain fatty acids or the lipids containing these fatty acids as components, wherein: protein (A) is a protein consisting of the amino acid sequence set forth in SEQ ID NO: 1; and protein (B) is a protein consisting of an amino acid sequence having 70% or more identity with the amino acid sequence of the protein (A), and having ?-ketoacyl-ACP synthase activity.
    Type: Grant
    Filed: August 2, 2016
    Date of Patent: July 2, 2019
    Assignee: Kao Corporation
    Inventors: Tatsuro Ozaki, Shinji Sugihara
  • Patent number: 10299502
    Abstract: The invention relates to a method for producing an extract of a matrix of vegetable origin, particularly a plant, involving a solid-liquid extraction by means of an aqueous solution containing at least one, preferably agro-sourced, non-ionic amphiphilic compound, at a concentration at least equal to the minimum hydrotrope concentration thereof.
    Type: Grant
    Filed: March 18, 2016
    Date of Patent: May 28, 2019
    Assignee: PIERRE FABRE DERMO-COSMETIQUE
    Inventors: Mathieu Leti, Anne Mandeau
  • Patent number: 10287234
    Abstract: The invention provides a HYA derivative having superior physiological functions intrinsic to HYA and permitting easy ingestion and easy handling, and use thereof. In particular, the invention relates to a triglyceride in which at least one fatty acid forming an ester bond with a hydroxy group of glycerol is 10-hydroxy-cis-12-octadecenoic acid, or an optical isomer thereof, and a composition (edible fat or oil, food, medicament, cosmetic etc.) containing same.
    Type: Grant
    Filed: June 2, 2016
    Date of Patent: May 14, 2019
    Assignees: NITTO PHARMACEUTICAL INDUSTRIES, LTD., KYOTO UNIVERSITY
    Inventors: Yasunori Yonejima, Jun Ogawa, Shigenobu Kishino
  • Patent number: 10280432
    Abstract: The invention relates to genetically modified agricultural plants with increased oil content in vegetative tissues, as well as to expression systems, plant cells, seeds and vegetative tissues related thereto.
    Type: Grant
    Filed: April 13, 2017
    Date of Patent: May 7, 2019
    Assignee: Board of Trustees of Michigan State University
    Inventors: Christoph Benning, Sanjaya
  • Patent number: 10280428
    Abstract: The present invention provides compositions and methods for the genetic manipulation of Algal cells. The compositions and methods allow enhanced transfer of genetic material into Algal cells and the cloning and selection of genetically modified cells. Expression of proteins encoded by the genetic material will be enhanced by the methods and compositions of the invention.
    Type: Grant
    Filed: December 13, 2017
    Date of Patent: May 7, 2019
    Assignee: LIFE TECHNOLOGIES CORPORATION
    Inventors: Farzad Haerizadeh, Todd Peterson, Wen Chen, Ewa Lis
  • Patent number: 10266837
    Abstract: The present invention relates to the field of plant molecular biology. More particularly, the present invention relates to the isolation of nucleic acids encoding terpene synthases (TPSs), including a novel, multifunctional TPS identified herein as CoTPS2.
    Type: Grant
    Filed: October 19, 2015
    Date of Patent: April 23, 2019
    Assignee: TEMASEK LIFE SCIENCES LABORATORY LIMITED
    Inventors: In-cheol Jang, Mi Jung Kim
  • Patent number: 10260112
    Abstract: The present invention discloses novel oligonucleotide primer sequences BC1, BC2 and BC3 for the identification of Bacillus coagulans. The invention also discloses a PCR based method for the identification of Bacillus coagulants using the aforesaid primers, wherein positive amplification with primer sets BC1, BC2 and negative amplification with primer set BC3 confirms the presence of Bacillus coagulans.
    Type: Grant
    Filed: September 27, 2016
    Date of Patent: April 16, 2019
    Assignee: SAMI LABS LIMITED
    Inventors: Muhammed Majeed, Kalyanam Nagabhushanam, Sivakumar Arumugam, Furqan Ali
  • Patent number: 10259833
    Abstract: Provided herein is technology relating to natural lipids containing non-?-oxidizable fatty acids and particularly, but not exclusively, to compositions and methods related to the production and use of natural lipids containing non-?-oxidizable fatty acids.
    Type: Grant
    Filed: October 5, 2017
    Date of Patent: April 16, 2019
    Assignee: Bergen Teknologioverføring AS
    Inventor: Jan Remmereit
  • Patent number: 10240172
    Abstract: The present invention relates to the field of methods for purifying fatty acid ethyl esters. According to the present invention, a method for obtaining a ?3 fatty acid ethyl ester, such as EPA and DHA, each as a high purity product at a high yield is provided. In the method according to the present invention, a raw material fat including EPA and DHA is treated with a lipolytic enzyme and ethyl-esterification is performed as needed; the treated substance is fractionated into a glyceride fraction and a free fatty acid fraction; a fraction comprising more EPA ester and a fraction comprising DHA ester are obtained from the respective fractions; the fraction comprising more EPA ester is purified to prepare a high-purity EPA ester; and the fraction comprising more DHA ester is purified to prepare a high-purity DHA ester.
    Type: Grant
    Filed: August 18, 2014
    Date of Patent: March 26, 2019
    Assignee: Bizen Chemical Co., Ltd.
    Inventors: Yoshio Shimizu, Keisuke Uryu, Tetsuro Taira, Jun Fujii
  • Patent number: 10233469
    Abstract: Process for extracting volatile fatty acids (VFA), organic molecules called precursors produced by microorganisms (M) in a fermentation reactor (2) by anaerobic fermentation (3) using fermentable biomass (1), said molecules being fermentative metabolites, comprising at least the following steps: a) selecting an extraction means (8) from extraction means that is, at least, insoluble in the fermentation medium and the processing conditions of which preserve the capacity of the microorganisms (M) present in the fermentation medium to produce the molecules, b) bringing the chosen extraction means (8) into contact (9) with the fermentation medium without interrupting the fermentation (3), c) recovering (12) the extracted molecules, at a pH lower than 4.5, by the extraction means (8) outside the fermentation reactor (2). The invention also relates to an installation for implementing the method.
    Type: Grant
    Filed: July 17, 2015
    Date of Patent: March 19, 2019
    Assignee: AFYREN
    Inventors: Régis Nouaille, Jérémy Pessiot
  • Patent number: 10214756
    Abstract: There is provided a method for generating an oil/fat component by means of culturing algae, in which marine algae belonging to chlamydomonas are cultured in a culture medium containing sea salt.
    Type: Grant
    Filed: March 17, 2014
    Date of Patent: February 26, 2019
    Assignees: National University Corporation KOBE University, Inter-University Research Institute Corporation National Institutes of Natural Sciences, DIC Corporation
    Inventors: Akihiko Kondo, Tomohisa Hasunuma, Shih-hsin Ho, Jun Minagawa, Haruo Nishie, Hiroyuki Taroda, Jo-Shu Chang
  • Patent number: 10201514
    Abstract: There are provided microorganisms having a property of producing a lipid containing unsaturated fatty acids as constituent fatty acids and extracellularly secreting the produced lipid encapsulated in lipid particles, methods of screening said microorganisms, as well as methods of efficiently producing a fatty acid-containing lipid using said microorganisms. Furthermore, there are provided lipid particles encapsulating a lipid containing unsaturated fatty acids, and foods, cosmetics, and animal feeds comprising said lipid particles added thereto. Artificially treated microorganisms or microorganisms collected from nature are grown on a solid medium, and microbial strains that form lipid particles at the periphery of the colonies and/or microbial strains that, when cultured in a transparent liquid medium, make the culture liquid cloudy are selected.
    Type: Grant
    Filed: March 2, 2016
    Date of Patent: February 12, 2019
    Assignee: SUNTORY HOLDINGS LIMITED
    Inventors: Kengo Akimoto, Hiroshi Kawashima, Sakayu Shimizu
  • Patent number: 10196663
    Abstract: Provided are methods and yeast cultures for producing glycolipids and glycolipid compositions.
    Type: Grant
    Filed: March 30, 2015
    Date of Patent: February 5, 2019
    Assignee: The Regents of the University of California
    Inventors: Kyria Boundy-Mills, Luis Antonio Garay Almada, Irnayuli Sitepu, J. Bruce German
  • Patent number: 10190131
    Abstract: The invention relates to a method for producing eicosapentanoic acid, docosapentanoic acid and/or docohexanoic acid in transgenic plants. According to said method, the plant is provided with at least one nucleic acid sequence coding for a polypeptide with a ?6 desaturase activity, at least one nucleic acid sequence coding for a polypeptide with a ?6 elongase activity, at least one nucleic acid sequence coding for a polypeptide with a ?5 desaturase activity, and at least one nucleic acid sequence coding for a polypeptide with a ?5 elongase activity, the nucleic acid sequence coding for a polypeptide with a ?5 elongase activity being modified in relation to the nucleic acid sequence in the organism from which the sequence originates, such that it is adapted to the codon use in at least one type of plant. For the production of docosahexanoic acid, at least one nucleic acid sequence coding for a polypeptide with a ?4 desaturase activity is also introduced into the plant.
    Type: Grant
    Filed: February 21, 2007
    Date of Patent: January 29, 2019
    Assignee: BASF Plant Science GmbH
    Inventors: Petra Cirpus, Jörg Bauer, Xiao Qiu, Guohai Wu, Bifang Cheng, Martin Truksa, Tom Wetjen
  • Patent number: 10160989
    Abstract: One of the most important technical barriers for algal biofuel is the substantial harvesting and extracting cost. The present invention includes a system and method for the pelletization of single cell microalgae through co-cultivation with filamentous fungi to enable the low cost separation of microalgae from liquid medium and to significantly increase the biomass and lipid yield. The approach represents a simple and straightforward method to significantly reduce the cost for algae-based biofuel production and enable the economically viable algae biofuel production.
    Type: Grant
    Filed: January 9, 2012
    Date of Patent: December 25, 2018
    Assignee: RENEWUEL LLC
    Inventors: Joshua (Shuhua) Yuan, Shangxian Xie
  • Patent number: 10138504
    Abstract: The present invention provides for novel metabolic pathways to convert biomass and other carbohydrate sources to malonyl-CoA derived products, such as hydrocarbons and other bioproducts, under anaerobic conditions and with the net production of ATP. More specifically, the invention provides for a recombinant microorganism comprising one or more native and/or heterologous enzymes that function in one or more engineered metabolic pathways to achieve conversion of a carbohydrate source to, e.g., long-chain hydrocarbons and hydrocarbon derivatives, wherein the one or more native and/or heterologous enzymes is activated, upregulated, downregulated, or deleted. The invention also provides for processes to convert biomass to malonyl-CoA derived products which comprise contacting a carbohydrate source with a recombinant microorganism of the invention.
    Type: Grant
    Filed: August 5, 2011
    Date of Patent: November 27, 2018
    Assignee: Lallemand Hungary Liquidity Management LLC
    Inventors: William Ryan Sillers, Shital A. Tripathi, Arthur J. Shaw, IV, Aaron Argyros, David A. Hogsett
  • Patent number: 10087496
    Abstract: A green alga variant having a dual-specificity tyrosine-phosphorylation regulated protein kinase activity that is reduced compared to a dual-specificity tyrosine-phosphorylation regulated protein kinase activity of a parental strain is provided. The green alga variant increases a total amount of a lipid production per unit time and per unit culture area compared to a total amount of a lipid production of the parental strain. A dual-specificity tyrosine-phosphorylation regulated protein kinase of the parental strain is a protein having an amino acid sequence with at least 50% sequence identity with the amino acid sequence of an active site and a substrate recognition site of SEQ ID NO: 4 and having the dual-specificity tyrosine-phosphorylation regulated protein kinase activity.
    Type: Grant
    Filed: November 7, 2014
    Date of Patent: October 2, 2018
    Assignee: DENSO CORPORATION
    Inventors: Jumpei Hayakawa, Yoko Ide, Shigeaki Harayama, Hidehiko Yasui
  • Patent number: 10065877
    Abstract: The present invention addresses the problem of providing a new microorganism that is useful for efficiently decomposing oils and fats, and a use for said microorganism. According to screening results, a new Yarrowia lipolytica having a high capacity to assimilate free fatty acids was successfully obtained. Efficient decomposition of oils and fats is achieved by causing the Yarrowia lipolytica to act under conditions in which fatty acids that are hydrolysis products of oils or fats are present, or under conditions in which oils or fats are decomposed into fatty acids and glycerol.
    Type: Grant
    Filed: January 16, 2013
    Date of Patent: September 4, 2018
    Assignee: Nissan Chemical Industries, Ltd.
    Inventors: Katsutoshi Hori, Masatake Fujioka
  • Patent number: 10023884
    Abstract: According to the present invention, a microorganism belonging to the genus Aurantiochytrium having an 18S rRNA gene consisting of the base sequence represented by any of SEQ ID NOS: 1 to 5; Aurantiochytrium sp. OH4 strain; a microorganism which is a mutant obtained from the above-mentioned microorganism as a parent strain and has a higher ability to produce DHA than the parent strain; or Aurantiochytrium sp. LTR23 strain is provided. Also, a method for producing a DHA-containing composition, DHA and DHA alkyl ester by a fermentation process using the above-mentioned microorganisms is provided.
    Type: Grant
    Filed: January 17, 2014
    Date of Patent: July 17, 2018
    Assignee: KYOWA HAKKO BIO CO., LTD.
    Inventors: Tetsuro Ujihara, Megumi Nagano, Kazuhiko Tabata
  • Patent number: 10006066
    Abstract: Provided is a cyanobacterium with improved productivity of fatty acid. A method for producing a modified cyanobacterium, comprising causing loss of functions of an AbrB-like transcriptional regulator and acyl-ACP synthetase in a cyanobacterium.
    Type: Grant
    Filed: January 30, 2015
    Date of Patent: June 26, 2018
    Assignees: Kao Corporation, Saitama University
    Inventors: Akihito Kawahara, Yukako Sonoike
  • Patent number: 9982269
    Abstract: It relates to a Mortierella alpine ATCC32222 uracil auxotroph strain and a construction method thereof. In the present invention, Mortierella alpine ATCC32222 is used as a material and undergoes gene knockout through an Agrobacterium tumefaciens mediated genetic manipulation technology, to obtain the Mortierella alpine uracil auxotroph. The method is of great significance for the basic theoretic researches of the oil producing fungus Mortierella alpine ATCC32222 and product development.
    Type: Grant
    Filed: February 21, 2014
    Date of Patent: May 29, 2018
    Assignee: JIANGNAN UNIVERSITY
    Inventors: Wei Chen, Haiqin Chen, Yongquan Chen, Guangfei Hao, Zhennan Gu, Jianxin Zhao, Hao Zhang, Xiaoyun Huang, Kai Du, Shanshan Zhao
  • Patent number: 9963722
    Abstract: A bacterial strain secreting fatty acids, the strain inducing fatty acids to be extracellularly secreted by using phospholipase expressed in the periplasmic space of cell. When a method of producing fatty acids by using the bacterial strain secreting fatty acids is used, fatty acids extracellularly secreted are continuously obtained without apoptosis, leading to lower costs and higher production efficiency. Phospholipase, unlike thioesterase, which is a typical fatty-acid degrading enzyme, decomposes phospholipid to produce free fatty acids. Accordingly, by using the substrate specificity of two different phospholipases, a fatty acid having a specific composition can be selectively produced. Unlike in a typical method in which fat is obtained from cells or tissues, fatty acids secreted during cell growth are obtainable by biding to a hydrophobic material without an extraction process using an organic solvent in large quantities.
    Type: Grant
    Filed: June 3, 2015
    Date of Patent: May 8, 2018
    Assignee: SOGANG UNIVERSITY RESEARCH FOUNDATION
    Inventors: Jeong Kug Lee, Eui Jin Kim, Xiaomeng Tong, Eun Kyung Oh
  • Patent number: 9944959
    Abstract: A microbial cell is used for producing at least one fatty acid ester, wherein the cell is genetically modified to contain (i) at least one first genetic mutation that enables the cell to produce at least one fatty acid and/or acyl coenzyme A (CoA) thereof by increased enzymatic activity in the cell relative to the wild type cell of malonyl-CoA dependent and malonyl-ACP independent fatty acyl-CoA metabolic pathway, wherein the fatty acid contains at least 5 carbon atoms; and (ii) a second genetic mutation that increases the activity of at least one wax ester synthase in the cell relative to the wild type cell and the wax ester synthase has sequence identity of at least 50% to a polypeptide of SEQ ID NO: 1-8 and combinations thereof or to a functional fragment of any of the polypeptides for catalyzing the conversion of fatty acid and/or acyl coenzyme A thereof to the fatty acid ester.
    Type: Grant
    Filed: September 2, 2015
    Date of Patent: April 17, 2018
    Assignee: CARGILL, INCORPORATED
    Inventors: Katrin Grammann, Jan Wolter, Liv Reinecke, Steffen Schaffer, Eileen C. Spindler, Wendy K. Ribble, Brittany L. Prather, Catherine B. Poor, Tanya Warnecke Lipscomb, Hans H. Liao, Dave A. Hogsett, Ronald J. Evans
  • Patent number: 9938545
    Abstract: The present invention relates to a genetically engineered algae strain in which the expression of the CGI-58 gene or homologous gene thereof is silenced. The present invention further relates to a method of triacylglycerol accumulation using said genetically engineered diatom and/or diatom strain.
    Type: Grant
    Filed: July 21, 2014
    Date of Patent: April 10, 2018
    Assignees: FERMENTALG, CENTRE NATIONAL DE LA RECHERCHE SCIENTIFIQUE(C.N.R.S), COMMISSARIAT A L'ENERGIE ATOMIQUE ET AUX ENERGIES ALTERNATIVES
    Inventors: Pierre Calleja, Marina Leterrier, Eric Marechal
  • Patent number: 9938544
    Abstract: The technology relates in part to biological methods for producing a fatty dicarboxylic acid and engineered microorganisms capable of such production.
    Type: Grant
    Filed: July 7, 2017
    Date of Patent: April 10, 2018
    Assignee: Verdezyne, Inc.
    Inventors: Tom Beardslee, Stephen Picataggio, L. Dudley Eirich, Jose Miguel Laplaza
  • Patent number: 9908917
    Abstract: The present invention relates to the expression of polypeptides using Yarrowia lipolytica, in particular the secretion of expressed polypeptides into either the extracellular space or the surface of the Y. lipolytica host cell wall. The invention also extends to the use of the polypeptides so expressed in biotechnological applications. The present invention provides an expression construct for the expression of polypeptides using at least a single Yarrowia lipolytica yeast cell, the expression construct having at least one expression cassette, the expression cassette including an acid extracellular protease secretion signal sequence and flanking zeta sequence recombination sites.
    Type: Grant
    Filed: September 30, 2015
    Date of Patent: March 6, 2018
    Assignee: UNIVERSITY OF THE FREE STATE
    Inventors: Robert Richard Bragg, Charlotte Enastacia Boucher, Chrispian William Theron, Arina Corli Hitzeroth
  • Patent number: 9890403
    Abstract: The present disclosure relates to methods for producing microbial lipids. The present disclosure also relates to methods for producing microbial lipids using inhibitors obtainable from lignocellulosic materials to suppress the proliferation of unwanted microorganisms in the fermentation broth. The method can therefore reduce the risk of having contaminating microbes establish in the system and the cultivation and thus higher yields of microbial lipids may be obtained.
    Type: Grant
    Filed: December 11, 2014
    Date of Patent: February 13, 2018
    Assignee: NESTE OYJ
    Inventors: Heidi Vainio, Mika Sipponen, Simo Laakso, Ossi Pastinen, Ilkka Lehtomäki, Perttu Koskinen, Miia Laamanen
  • Patent number: 9884883
    Abstract: Provided are organic solvent free methods for obtaining compositions comprising one or more rhamnolipids as well as compositions obtainable from said methods.
    Type: Grant
    Filed: January 11, 2016
    Date of Patent: February 6, 2018
    Assignee: Logos Technologies, LLC
    Inventors: Nattaporn Lohitharn, Daniel Derr
  • Patent number: 9879239
    Abstract: The disclosure relates to enzyme variants with improved ester synthase properties for the production of fatty acid esters. Further contemplated are recombinant host cells that express such variants, cell cultures comprising the recombinant host cells and fatty acid ester compositions produced by such recombinant host cells.
    Type: Grant
    Filed: March 15, 2013
    Date of Patent: January 30, 2018
    Assignee: REG LIFE SCIENCES, LLC
    Inventors: Andrew M. Shumaker, Bernardo M. Da Costa, Kevin Holden, Louis G. Hom, Tarah S. Baron, Noah Helman
  • Patent number: 9862977
    Abstract: Some aspects of this invention provide engineered microbes for oil production. Methods for microbe engineering and for use of engineered microbes are also provided herein. In some embodiments, microbes are provided that are engineered to modulate a combination of rate-controlling steps of lipid synthesis, for example, a combination of a step generating metabolites, acetyl-CoA, ATP or NADPH for lipid synthesis (a push step), and a step sequestering a product or an intermediate of a lipid synthesis pathway that mediates feedback inhibition of lipid synthesis (a pull step). Such push-and-pull engineered microbes exhibit greatly enhanced conversion yields and TAG synthesis and storage properties.
    Type: Grant
    Filed: November 19, 2014
    Date of Patent: January 9, 2018
    Assignee: Massachusetts Institute of Technology
    Inventors: Gregory Stephanopoulos, Mitchell Tai, Sagar Chakraborty
  • Patent number: 9856289
    Abstract: The invention provides peptides that can reactivate p53 mutants efficiently and specifically, as well as methods that allow the identification, selection and isolation of such peptides, in a precise, cost and time effective manner. In particular, there are provided mutant p53 reactivating peptides that can restore the native wild type p53 folding, and hence the tumor suppressor activity, to the mutant p53 protein. Such peptides are useful for treating various conditions and diseases in which p53 is mutated.
    Type: Grant
    Filed: February 4, 2016
    Date of Patent: January 2, 2018
    Assignee: Yeda Research and Development Co. Ltd.
    Inventors: Moshe Oren, Varda Rotter, Perry Tal
  • Patent number: 9848623
    Abstract: The present invention provides a process for growing eukaryotic microorganisms which are capable of producing lipids, in particular lipids containing polyenoic fatty acids. The present invention also provides a process for producing eukaryotic microbial lipids.
    Type: Grant
    Filed: January 3, 2012
    Date of Patent: December 26, 2017
    Assignee: DSM IP Assets B.V.
    Inventors: Richard B. Bailey, Don Dimasi, John M. Hansen, Peter J. Mirrasoul, Craig M. Ruecker, George T. Veeder, III, Tatsuo Kaneko, William R. Barclay
  • Patent number: 9834799
    Abstract: Methods for production of highly unsaturated fatty acids by marine microorganisms, including the heterotrophic marine dinoflagellate Crypthecodinium, using low levels of chloride ion are disclosed. Specifically, methods of increasing production of highly unsaturated fatty acids by marine microorganisms while growing in low chloride media by manipulating sodium ion and potassium ion levels. The invention also relates to methods of production of highly unsaturated fatty acids by marine organisms at low pH levels, and includes methods for generation of low pH tolerant strains.
    Type: Grant
    Filed: December 18, 2015
    Date of Patent: December 5, 2017
    Assignee: DSM IP Assets B.V.
    Inventors: Paul W Behrens, John M Thompson, Kirk Apt, Joseph W. Pfeifer, III, James P Wynn, James Casey Lippmeier, Jaouad Fichtali, Jon Hansen
  • Patent number: 9833484
    Abstract: The present invention relates to conjugated linoleic acid (CLA)-producing strains of probiotic bacteria. In particular, the present invention relates to a selection of bacterial strains belonging to the genus Bifidobacterium which were selected for their ability to produce conjugated linoleic acid (CLA) from linoleic acid (LA). Furthermore, the present invention relates to a food, dietetic or pharmaceutical composition comprising said bacterial strains capable of increasing the quantity of CLA in situ, i.e. inside the gastrointestinal tract.
    Type: Grant
    Filed: June 18, 2014
    Date of Patent: December 5, 2017
    Assignee: ALCE NORTH AMERICA, INC.
    Inventors: Giovanni Mogna, Gian Paolo Strozzi, Marco Pane
  • Patent number: 9796658
    Abstract: The present invention includes methods for producing and purifying esters of polyunsaturated fatty acids that include reacting a composition having triglycerides with polyunsaturated fatty acid residues in the presence of an alcohol and a base to produce an ester of a polyunsaturated fatty acid from the triglycerides. The composition can be a polyunsaturated fatty acid-containing composition that has not been conventionally processed. The reacted composition can be further processed by distillation.
    Type: Grant
    Filed: January 13, 2014
    Date of Patent: October 24, 2017
    Assignee: DSM IP Assets B.V.
    Inventors: Krishna Raman, Jung Lee, Neil Leininger, Chris Luigart
  • Patent number: 9782378
    Abstract: There are provided microorganisms having a property of producing a lipid containing unsaturated fatty acids as constituent fatty acids and extracellularly secreting the produced lipid encapsulated in lipid particles, methods of screening said microorganisms, as well as methods of efficiently producing a fatty acid-containing lipid using said microorganisms. Furthermore, there are provided lipid particles encapsulating a lipid containing unsaturated fatty acids, and foods, cosmetics, and animal feeds comprising said lipid particles added thereto. Artificially treated microorganisms or microorganisms collected from nature are grown on a solid medium, and microbial strains that form lipid particles at the periphery of the colonies and/or microbial strains that, when cultured in a transparent liquid medium, make the culture liquid cloudy are selected.
    Type: Grant
    Filed: December 1, 2006
    Date of Patent: October 10, 2017
    Assignee: SUNTORY HOLDINGS LIMITED
    Inventors: Kengo Akimoto, Hiroshi Kawashima, Sakayu Shimizu
  • Patent number: 9745538
    Abstract: Provided herein are methods of recovering oil from microorganisms. The methods are useful, for example, in obtaining nutritional oils and/or lipid biofuels. The methods of recovering oil described herein include contacting a population of microorganisms with one or more enzymes under conditions that cause disruption of the microorganisms, concentrating the disrupted microorganisms, and extracting lipids from the disrupted microorganisms at high temperature in the presence of a salt and in the absence of solvent.
    Type: Grant
    Filed: December 19, 2014
    Date of Patent: August 29, 2017
    Assignee: MARA RENEWABLES CORPORATION
    Inventors: Dorothy A. Dennis, Roberto E. Armenta
  • Patent number: 9745362
    Abstract: A seed-specific expression vector and its construction methods and applications are disclosed. A fusion protein expression cassette consisting of Arachis hypogaea oleosin gene-apolipopoprotein A-IMilano (A-IM) gene driven by Brassica napus oleosin gene promoter is inserted between the HindIII and SacI sites of a plant binary expression vector pBI121, obtaining the plant expression vector pBINOA of the invention. In addition, a method for producing apolipoprotein A-IMilano is provided, in which the expression vector is used to transform oil sunflower which is used as a plant bioreactor. The method can not only improve the yield of apolipoprotein A-IMilano, but also greatly reduce production costs, and is suitable for industrial production.
    Type: Grant
    Filed: December 9, 2010
    Date of Patent: August 29, 2017
    Inventors: Shengjun An, Xiqing Chai, Kunsheng Wang, Tiemei Shao, Zhan Jiao, Xin Wen, Xue Li, Pei Liu, Haigang Lu, Yunyu Chen, Liangyuan Hu, Haimin Xu, Chenggang Yu
  • Patent number: 9745539
    Abstract: Provided herein are methods of recovering oil from microorganisms. The methods are useful, for example, in obtaining nutritional oils and/or lipid biofuels. The methods of recovering oil described herein include contacting a population of microorganisms with one or more enzymes under conditions that cause disruption of the microorganisms, concentrating the disrupted microorganisms, and extracting lipids from the disrupted microorganisms at high temperature in the presence of a salt and in the absence of solvent.
    Type: Grant
    Filed: September 23, 2015
    Date of Patent: August 29, 2017
    Inventors: Dorothy A. Dennis, Roberto E. Armenta
  • Patent number: 9738913
    Abstract: The technology relates in part to biological methods for producing a fatty dicarboxylic acid and engineered microorganisms capable of such production.
    Type: Grant
    Filed: July 5, 2012
    Date of Patent: August 22, 2017
    Assignee: Verdezyne, Inc.
    Inventors: Tom Beardslee, Jose Miguel LaPlaza
  • Patent number: 9719117
    Abstract: The invention provides a whole cell catalyst which expresses a recombinant ?-dioxygenase or the combination of a recombinant fatty acid reductase and a phosphopantetheinyl transferase phosphopantetheinylating the fatty acid reductase, and which in addition to the ?-dioxygenase and/or the combination of fatty acid reductase and phosphopantetheinyl transferase expresses a transaminase, characterized in that the phosphopantetheinyl transferase and/or transaminase is preferably recombinant; and a method for the conversion of a fatty acid, ?-hydroxy fatty acid, ?-oxo fatty acid or a monoester thereof to an amine, comprising oxidation of the fatty acid, ?-hydroxy fatty acid, ?-oxo fatty acid or the monoester thereof to an oxidation product by contacting with an alkane hydroxylase and/or alcohol dehydrogenase, contacting the oxidation product with a phosphopantetheinylated fatty acid reductase or a ?-dioxygenase to give an aldehyde, and contacting the aldehyde with a transaminase.
    Type: Grant
    Filed: December 18, 2013
    Date of Patent: August 1, 2017
    Assignee: Evonik Degussa
    Inventors: Steffen Schaffer, Jasmin Gielen, Mirja Wessel, Hans-Georg Hennemann, Harald Haeger, Thomas Haas, Wilfried Bluemke
  • Patent number: 9701990
    Abstract: A method of culturing a microorganism capable of producing at least any one of a highly unsaturated fatty acid or a compound containing a highly unsaturated fatty acid as a constituent fatty acid in a culture medium containing at least a carbon source and a nitrogen source, using an aeration-agitation culture device capable of adjusting and controlling an agitation power and an aeration amount. The method comprises the steps of performing mechanical agitation for a predetermined time after start of culture, where the agitation power per unit liquid amount is 269 (W/m3) or less, and after the predetermined time has passed, adjusting and controlling at least any one of the maximum aeration amount and a maximum power required for agitation to a range which satisfies that KLA (=(P/V)0.95Vs0.67) is 59 or more, an air flow rate parameter Vs0.67 is 0.075 or more, and a required agitation power parameter (P/V)0.
    Type: Grant
    Filed: September 5, 2005
    Date of Patent: July 11, 2017
    Assignee: NIPPON SUISAN KAISHA, LTD.
    Inventor: Kenichi Higashiyama
  • Patent number: 9701923
    Abstract: Extracting lipids from a feedstock includes contacting a feedstock including water, lipids, and solid organic matter with an organic solvent to yield a mixture; separating the mixture to yield a solid and a liquid; and desolventizing the solid to yield a solid product and a fluid product. The organic solvent may include a polar organic solvent, a non-polar organic solvent, or a combination thereof. The lipids in the mixture are in the liquid state. The solid product has a lipids content of 20 wt % or less. In some cases, the number-weighted particle size distribution of the solid organic matter has a mean dimension of 1 mm or less, the lipids content of the feedstock is at least 20 wt %, the water content of the feedstock is 10 wt % or less or 5 wt % or less, or a combination thereof.
    Type: Grant
    Filed: October 23, 2015
    Date of Patent: July 11, 2017
    Assignee: Darling Ingredients Inc.
    Inventors: Martin W. Griffin, John L. Crowley
  • Patent number: 9701989
    Abstract: Recombinant microbial cells are disclosed herein that produce an oil comprising at least 28 percent eicosapentaenoic acid (EPA) measured as a weight percent of dry cell weight. These cells may comprise a polynucleotide sequence encoding an active acyl-CoA:lysophosphatidylcholine acyltransferase (LPCAT) comprising at least one amino acid mutation in a membrane-bound O-acyltransferase motif. In addition, the cells may comprise a down-regulation of an endogenous polynucleotide sequence encoding Sou2 sorbitol utilization protein, and/or one or more polynucleotides encoding phospholipid:diacylglycerol acyltransferase (PDAT), delta-12 desaturase, a dihomo-gamma-linolenic acid (DGLA) synthase multizyme, delta-8 desaturase, malonyl-CoA synthetase (MCS), or acyl-CoA:lysophosphatidic acid acyltransferase (LPAAT). Also disclosed are methods of using the recombinant microbial cells to produce oil containing omega-3 polyunsaturated fatty acids such as EPA.
    Type: Grant
    Filed: December 18, 2013
    Date of Patent: July 11, 2017
    Assignee: E I DU PONT DE NEMOURS AND COMPANY
    Inventors: Quinn Qun Zhu, Seung-Pyo Hong, Pamela L Sharpe, Narendra S Yadav, Xiaochun Fan, Michael Dauner
  • Patent number: 9695450
    Abstract: The amino acid and nucleic acid sequences of a ?5-desaturase enzyme and a ?8-desaturase enzyme are disclosed. The nucleic acid sequences can be used to design recombinant DNA constructs and vectors. These vectors can then be used to transform various organisms, including for example, plants and yeast. The transformed organisms will then produce polyunsaturated fatty acids. The amino acid sequences are useful for generating enzyme-specific antibodies that are useful for identifying the desaturases.
    Type: Grant
    Filed: June 18, 2014
    Date of Patent: July 4, 2017
    Assignee: Washington State University
    Inventors: John A. Browse, James G. Wallis, Jennifer L. Watts
  • Patent number: 9677027
    Abstract: The invention relates to a composition comprising at least one phospholipid-cleaving enzyme. The invention further relates to a method for degumming raw oils using the composition according to the invention and to the use of the composition according to the invention to degum raw oils.
    Type: Grant
    Filed: February 18, 2013
    Date of Patent: June 13, 2017
    Assignee: CLARIANT PRODUKTE (DEUTSCHLAND) GMBH
    Inventors: Ulrich Sohling, Paul Bubenheim, Kirstin Suck
  • Patent number: 9670454
    Abstract: Methods of inducing or increasing lipid and protein production in microalgae are disclosed. Methods of enhancing microalgae biomass are also disclosed. The methods may comprise inoculating a culture media with microalgae and propagating the microalgae under heterotrophic growth conditions. The heterotrophic growth conditions may comprise inhibiting exposure of the inoculated culture media to light. The methods may further comprise delivering oxygen into the inoculated culture media. Additionally, lipids, proteins, and other cellular components may be isolated and purified from microalgae cultivated under the disclosed methods.
    Type: Grant
    Filed: August 8, 2016
    Date of Patent: June 6, 2017
    Assignee: Entech, LLC
    Inventors: John Whitman, Robert G. Barton, Michael Ripka, III, Michael Ripka, Jr., Robert L. Harris
  • Patent number: 9668942
    Abstract: Nutritional formulas comprising long-chain polyunsaturated fatty acids (LC-PUFAs) are provided, along with methods and devices for preparing and/or administering nutritional formulas. In some embodiments, a percentage of the LC-PUFAs in the nutritional formula are in the form of monoglycerides and/or free fatty acids. In some embodiments, the nutritional formulas do not comprise added lipase. Also provided are methods for providing nutrition to a subject, methods for improving fat absorption, methods for improving cognitive ability, methods for preventing chronic lung disease, and methods for reducing the length of time a patient requires total parenteral nutrition.
    Type: Grant
    Filed: February 14, 2013
    Date of Patent: June 6, 2017
    Assignee: ALCRESTA THERAPEUTICS, INC.
    Inventors: Alexey L. Margolin, Robert Gallotto, Bhami Shenoy
  • Patent number: 9598696
    Abstract: The present invention discloses a process for increasing the production of free fatty acids at high yield (close to maximum theoretical yield), with various fatty acid compositions and various percentage of fatty acids accumulated intracellularly. This invention will enable the efficient production of other products derived from free fatty acids and/or products that can be branched out from the fatty acid synthesis pathways.
    Type: Grant
    Filed: October 16, 2012
    Date of Patent: March 21, 2017
    Assignee: William Marsh Rice University
    Inventors: Ka-Yiu San, Mai Li