Fat; Fatty Oil; Ester-type Wax; Higher Fatty Acid (i.e., Having At Least Seven Carbon Atoms In An Unbroken Chain Bound To A Carboxyl Group); Oxidized Oil Or Fat Patents (Class 435/134)
  • Patent number: 11447799
    Abstract: Aqueous fermentation feedstock and method of producing same. The feedstock includes glucose and dextrose oligomers, wherein (i) glucose concentration is in a range between 10 gram/Liter (g/L) and 150 g/L; (ii) dextrose oligomers concentration is in a range between 50 g/L and 300 g/L; and optionally (iii) slurried particles of less than 0.5 micron; (iv) slurried particles of more than 0.5 micron, wherein a content of such suspended particles of more than 0.5 micron is less than 30 g/L; (v) ash at a concentration in a range between 20 g/L and 50 g/L; (vi) lactate at a concentration in a range between 0.5 g/L and 10 g/L; (vii) protein at a concentration in a range between 5 g/L and 50 g/L; (viii) corn oil at a concentration of less than 10 g/L; and/or (ix) glycerol at a concentration in a range between 1 g/L and 30 g/L.
    Type: Grant
    Filed: September 10, 2020
    Date of Patent: September 20, 2022
    Assignee: SUPERBREWED FOOD INC.
    Inventors: Bryan P. Tracy, John Randall Phillips, Daniel Knox Mitchell
  • Patent number: 11439691
    Abstract: The present invention relates to an aqueous solution comprising burlulipase which is characterized in that it contains calcium ions. In particular, it also relates to corresponding pharmaceuticals. Such pharmaceuticals are suitable for the treatment of exocrine pancreatic insufficiency. These pharmaceuticals are particularly suitable for treating patients having cystic fibrosis and in pediatrics. Another aspect of the present invention relates to container-packaged solutions and pharmaceuticals as mentioned above, which are containers produced by the blow-fill-seal method, and to methods for producing such packaged solutions and pharmaceuticals.
    Type: Grant
    Filed: March 5, 2018
    Date of Patent: September 13, 2022
    Assignee: NORDMARK PHARMA GMBH
    Inventors: Richard Fuhrherr, Annika Müller-Lucks, Katharina Rützel, Jan Lüdemann, Martin Werner
  • Patent number: 11434506
    Abstract: The present invention relates to the production of compounds comprised in pheromones, in particular moth pheromones, such as desaturated fatty alcohols and desaturated fatty alcohol acetates and derivatives thereof, from a yeast cell.
    Type: Grant
    Filed: December 15, 2017
    Date of Patent: September 6, 2022
    Assignee: Danmarks Tekniske Universitet
    Inventors: Irina Borodina, Carina Holkenbrink, Marie Inger Dam, Christer Löfstedt, Baojian Ding, Hong-Lei Wang
  • Patent number: 11421254
    Abstract: A method for oxidizing an alkyl, including a) contacting the alkyl with an aqueous solution comprising a microorganism where the microorganism has a reduced fatty acid degradation capacity compared to its wild type, wherein the fatty acid degradation capacity is reduced by deletion, inhibition or inactivation of a gene encoding an enzyme involved in the ?-oxidation pathway; and the microorganism expresses a recombinant alkane oxidase, and b) contacting the aqueous solution from a) with a water-immiscible organic solvent.
    Type: Grant
    Filed: October 7, 2020
    Date of Patent: August 23, 2022
    Assignee: Evonik Operations GmbH
    Inventors: Thomas Haas, Oliver Thum, Jan Christoph Pfeffer, Philip Engel, Christian Gehring, Markus Poetter
  • Patent number: 11406602
    Abstract: Medicaments and therapeutic compositions comprise Red Yeast Rice extract and omega-3 polyunsaturated fatty acids and/or derivatives thereof, e.g., DHA, derivatives of DHA, EPA, derivatives of EPA or mixtures thereof. One source of the fatty acids or derivatives thereof is fish oil. The compositions are useful for lowering cholesterol and/or triglyceride levels in a subject.
    Type: Grant
    Filed: March 23, 2020
    Date of Patent: August 9, 2022
    Assignee: NORDIC NATURALS, INC.
    Inventor: Joar Opheim
  • Patent number: 11375734
    Abstract: The compositions, method, system, and process of the invention provide increased circulating lipids, including serum triglycerides, in animals by utilizing oleaginous microorganisms as an oral supplement for the animals to increase available energy, enhance probiotics health, and provide enhanced immune system protection against pathogens in the animals.
    Type: Grant
    Filed: April 21, 2016
    Date of Patent: July 5, 2022
    Assignees: Mississippi State University, The United States of America, as Represented by the Secretary of Agriculture
    Inventors: Janet R. Donaldson, Jeffrey A. Carroll
  • Patent number: 11332576
    Abstract: The invention relates to a block copolymer derived from at least one ethylene oxide and/or propylene oxide monomer containing 14C. The present invention also relates to a method for preparing such a block copolymer.
    Type: Grant
    Filed: April 5, 2011
    Date of Patent: May 17, 2022
    Assignee: ARKEMA FRANCE
    Inventors: Frederic Malet, Guillaume Le, Julien Jouanneau
  • Patent number: 11324234
    Abstract: According to the invention, it was found that a feedstuff containing an Aurantiochytrium biomass brings about a significant increase in growth in fish.
    Type: Grant
    Filed: September 22, 2015
    Date of Patent: May 10, 2022
    Assignee: Evonik Operations GmbH
    Inventors: Amelia Claudia Silva, Stefan Eils, Horst Priefert, Christian Rabe
  • Patent number: 11326151
    Abstract: This disclosure provides a genetically-modified bacterium from the genus Pseudomonas that comprises an exogenous nucleic acid encoding an enoyl-CoA reductase and an exogenous nucleic acid encoding an acyl-CoA reductase that produces medium chain length alcohols. The disclosure further provides methods for producing medium chain alcohols using such genetically-modified bacterium. This disclosure provides a renewable, bio-based production platform for valuable mcl-alcohols that have a wide range of industrial applications. Current production of mcl-alcohols typically occurs through the hydrogenation of plant oils and waxes. This process leads to issues of deforestation and is largely unsustainable. Utilizing waste lignin streams as the carbon source provides a more sustainable feedstock that can be generated from plant waste like corn stover. Along with this, the use of lignin avoids competition with food resources as traditional starch and sugar feedstocks.
    Type: Grant
    Filed: July 21, 2020
    Date of Patent: May 10, 2022
    Assignee: UT-Battelle, LLC
    Inventors: Adam M. Guss, Joshua R. Elmore, Jay D. Huenemann
  • Patent number: 11306300
    Abstract: The present invention provides a transformed microorganism capable of displaying ?-galactosidase on its surface layer. Also provided is a method for producing an alcohol, which includes the step of culturing the transformed microorganism in a culture medium containing a material that contains an oligosaccharide ?-1,6 linked ?-galactose. Also provided is a method for producing lactic acid using such a transformed microorganism together with a material that contains an oligosaccharide ?-1,6 linked ?-galactose. According to the present invention, a microorganism can be provided, which can degrade an oligosaccharide containing ?-1,6 linked ?-galactose, which may occur in soybean molasses.
    Type: Grant
    Filed: May 10, 2018
    Date of Patent: April 19, 2022
    Assignees: KANSAI CHEMICAL ENGINEERING CO., LTD., BIO-ENERGY CORPORATION
    Inventors: Hideo Noda, Shinji Hama
  • Patent number: 11306272
    Abstract: There are provided odorless shea based esters as an ingredient composition comprising: a) 81-97 wt % of at least one short chain alcohol alkyl ester, at least partially from a natural source, b) 3-19 wt % of triterpene esters where at least one is a cinnamic triterpene ester, and c) 1100 ppm or less of at least one short chain alcohol cinnamic ester. There is further provided a method of manufacturing the composition comprising a deodorization step. An advantage is that an odourless or an almost odourless composition can be provided.
    Type: Grant
    Filed: April 12, 2017
    Date of Patent: April 19, 2022
    Assignee: AAK AB (PUBL)
    Inventors: Jari Alander, Staffan Norberg
  • Patent number: 11306334
    Abstract: Provided is a method of producing at least one omega-hydroxyl fatty acid, the method comprising: (a) contacting at least one alkane with at least one recombinant yeast cell in an aqueous medium, wherein the yeast cell is capable of oxidising the alkane to the corresponding omega-hydroxyl fatty acid and the yeast cell comprises a reduced fatty acid degradation capacity.
    Type: Grant
    Filed: July 26, 2017
    Date of Patent: April 19, 2022
    Assignee: Evonik Operations GmbH
    Inventors: Thomas Haas, Markus Poetter, Li Li, Guoqing Tao, Dong Cao
  • Patent number: 11274322
    Abstract: A method of producing lipids, containing the steps of: culturing a transformant into which a gene encoding at least one protein selected from the group consisting of the following proteins (A) to (C) is introduced; and producing fatty acids or lipids containing the same as components: (A) a protein consisting of an amino acid sequence having at least one amino acid substitution selected from the group consisting of the following (A-1) to (A-11) in the amino acid sequence set forth in SEQ ID NO: 1, and having acyl-ACP thioesterase activity; (B) a protein consisting of an amino acid sequence having at least one amino acid substitution selected from the group consisting of the following (B-1) to (B-11) in an amino acid sequence having 85% or more identity with the amino acid sequence set forth in SEQ ID NO: 1, and having acyl-ACP thioesterase activity; and (C) a protein containing the amino acid sequence of the protein (A) or (B), and having acyl-ACP thioesterase activity: (A-1) substitution of isoleucine for l
    Type: Grant
    Filed: October 3, 2018
    Date of Patent: March 15, 2022
    Assignee: Kao Corporation
    Inventor: Akihito Kawahara
  • Patent number: 11248245
    Abstract: Processes for transesterifying wax esters. Implementations may include: providing a feedstock including wax esters, contacting the feedstock with a lipase, and catalytically transesterifying the wax esters in the feedstock with the lipase to form a transesterified product. An oxidative stability index (OSI) of the transesterified product may be greater than an OSI of the feedstock.
    Type: Grant
    Filed: December 19, 2014
    Date of Patent: February 15, 2022
    Assignee: International Flora Technologies, Ltd.
    Inventors: Jeff Addy, Clay Daly, James H. Brown
  • Patent number: 11220675
    Abstract: The present application relates to methods to improve biomass or lipid production in a microorganism from one or more fatty acid and one or more simple carbon co-substrates. Produced lipids may include unsaturated C6-C24 fatty acids, alcohols, aldehydes, and acetates which may be useful as final products or precursors to insect pheromones, fragrances, flavors, and polymer intermediates. The application further relates to recombinant microorganisms modified for improved production of biomass or lipid, or improved lipid selectivity. Also provided are methods of producing one or more lipid using the recombinant microorganisms, as well as compositions comprising the recombinant microorganisms and/or optionally one or more of the product lipid.
    Type: Grant
    Filed: May 2, 2019
    Date of Patent: January 11, 2022
    Assignee: Provivi, Inc.
    Inventors: Effendi Leonard, Micah Sheppard, Thomas Heel, Kathryn Christina Wu
  • Patent number: 11198891
    Abstract: Provided herein are eukaryotic microorganisms having a simple lipid profile comprising long chain fatty acids (LCFAs). Also provided are compositions and cultures comprising the eukaryotic microorganisms as well as methods of using the eukaryotic microorganisms.
    Type: Grant
    Filed: December 18, 2017
    Date of Patent: December 14, 2021
    Assignee: MARA RENEWABLES CORPORATION
    Inventors: Violeta Ugalde, Zachary Sun, Anthony Windust, Roberto E. Armenta
  • Patent number: 11198892
    Abstract: Provided is a method of producing an oil containing polyunsaturated fatty acids by using Schizochytrium sp. The method comprises: performing fermentation using Schizochytrium sp.; resuspending the resultant cells after fermentation in water, adding cellulase and neutral protease for enzymolysis; mixing the enzymatic hydrolyzate with n-hexane, shaking, extracting, centrifuging, and collecting the n-hexane phase; concentrating the n-hexane phase under reduced pressure to remove the n-hexane, and drying to obtain an oil; performing a first crystallization and a second crystallization, and then performing cold filtration to obtain a liquid oil after the second crystallization; and subjecting the liquid oil to deacidification and decolorization. By regulating fermentation conditions and conducting concentration processing on polyunsaturated fatty acids, the content of eicosapentaenoic acid in the Schizochytrium sp.
    Type: Grant
    Filed: May 20, 2020
    Date of Patent: December 14, 2021
    Assignee: INFINITUS (CHINA) COMPANY LTD.
    Inventors: Zehua Chen, Yong Zhou, Jian Tang, Yonghua Wang, Zheng Xiao, Chenfeng Jing, Dong Liang
  • Patent number: 11154076
    Abstract: The present invention is directed to isolated microorganisms as well as strains and mutants thereof, biomasses, microbial oils, compositions, and cultures; methods of producing the microbial oils, biomasses, and mutants; and methods of using the isolated microorganisms, biomasses, and microbial oils.
    Type: Grant
    Filed: February 6, 2018
    Date of Patent: October 26, 2021
    Assignee: DSM IP Assets B.V.
    Inventors: Kirk E. Apt, Paul Warren Behrens, Jon Milton Hansen, Joseph W. Pfeifer, III, Tracey Lynn Stahl, Ross Zirkle
  • Patent number: 11111510
    Abstract: The invention provides methods and systems for the production of lipid products from a gaseous substrate using a two stage fermentation process. The method comprises providing a gaseous substrate comprising CO, CO2 or H2 or mixtures thereof, to a first bioreactor containing a culture or one or more microorganisms, and fermenting the substrate to produce acetate. The acetate from the first bioreactor is then provided to a second bioreactor, where it is used as a substrate for fermentation to lipids by one or more microalgae.
    Type: Grant
    Filed: December 20, 2019
    Date of Patent: September 7, 2021
    Assignees: LanzaTech New Zealand Limited, DBT-IOC Centre for Advanced Bio-Energy Research
    Inventors: Sean Dennis Simpson, Sebastian Michal Bernasek, Deepak K Tuli
  • Patent number: 11053287
    Abstract: Methods for increasing carbon-based chemical product yield in an organism by increasing carbon uptake and/or altering a pathway to or from an overflow metabolite in the organism, nonnaturally occurring organisms having increased carbon-based chemical product yield with increased carbon uptake and/or an altered pathway to or from an overflow metabolite, and methods for producing a carbon-based chemical product with these organisms are provided.
    Type: Grant
    Filed: April 30, 2019
    Date of Patent: July 6, 2021
    Assignee: INV Nylon Chemicals Americas, LLC
    Inventors: Alexander Brett Foster, Ana Teresa dos Santos Brito Mendes Roberts, Arghya Barman, Jonathan Kennedy
  • Patent number: 11053557
    Abstract: An improved dry grind system and method for producing a sugar stream from grains or similar carbohydrate sources and/or residues, such as for biofuel production, using membrane filtration. In particular, a sugar/carbohydrate stream, which includes a desired Dextrose Equivalent (DE) where DE describes the degree of conversion of starch to dextrose (aka glucose) and/or has had removed therefrom an undesirable amount of unfermentable components, can be produced after saccharification and prior to fermentation (or other sugar conversion process) using membrane filtration, with such sugar stream being available for biofuel production, e.g., alcohol production, or other processes. In addition, the systems and methods also can involve the removal of certain grain components, e.g., corn kernel components, including protein, oil and/or fiber, prior to fermentation or other conversion systems. In other words, sugar stream production and/or grain component separation occurs on the front end of the system and method.
    Type: Grant
    Filed: March 15, 2018
    Date of Patent: July 6, 2021
    Assignee: Fluid Quip Technologies, LLC
    Inventors: Neal Jakel, Albert Pollmeier
  • Patent number: 11053523
    Abstract: A method of producing lipids, containing the steps of: culturing an alga belonging to the genus Nannochloropsis; and producing fatty acids or lipids containing the same as components; wherein expressions of genes encoding a ?12-desaturase and a ?6-desaturase are enhanced in the alga; and a transformant of an alga belonging to the genus Nannochloropsis, wherein expressions of genes encoding a ?12-desaturease and a ?6-desaturease are enhanced.
    Type: Grant
    Filed: August 28, 2017
    Date of Patent: July 6, 2021
    Assignee: Kao Corporation
    Inventor: Tatsuro Ozaki
  • Patent number: 11046635
    Abstract: Genetically engineered cells and microorganisms are provided that produce products from the fatty acid biosynthetic pathway (fatty acid derivatives), as well as methods of their use. The products are particularly useful as biofuels.
    Type: Grant
    Filed: April 16, 2018
    Date of Patent: June 29, 2021
    Assignee: GENOMATICA, INC.
    Inventors: Zhihao Hu, Fernando Valle
  • Patent number: 11046979
    Abstract: The present invention provides a mutant algal microorganism that has a mutation that causes attenuated expression of TrifuncB and/or TrifuncA and as a result produces more lipids than a control algal microorganism. The mutant algal microorganism can further include a mutation in a gene encoding a peroxisomal beta-oxidation pathway protein, such as an ACO1 or PXA1 gene, or a glyoxylate pathway protein, such as an ICL1 gene, that results in attenuated expression and further increased lipid production. Furthermore, provided herein are methods of producing lipids using the mutant algal microorganisms and methods of making the mutant microorganisms.
    Type: Grant
    Filed: July 22, 2019
    Date of Patent: June 29, 2021
    Assignee: Synthetic Genomics, Inc.
    Inventors: Eric R. Moellering, Tom Carlson
  • Patent number: 11034987
    Abstract: An improved dry grind system and method for producing a sugar stream from grains or similar carbohydrate sources and/or residues, such as for biofuel production. In particular, a sugar/carbohydrate stream, which includes a desired Dextrose Equivalent (DE) where DE describes the degree of conversion of starch to dextrose (aka glucose) and/or has had removed therefrom an undesirable amount of unfermentable components, can be produced after saccharification and prior to fermentation (or other sugar conversion process), with such sugar stream being available for biofuel production, e.g., alcohol production, or other processes. In addition, the systems and methods also can involve the removal of certain grain components, e.g., corn kernel components, including protein, oil and/or fiber, prior to fermentation or other conversion systems. In other words, sugar stream production and/or grain component separation occurs on the front end of the system and method.
    Type: Grant
    Filed: July 3, 2018
    Date of Patent: June 15, 2021
    Assignee: Fluid Quip Technologies, LLC
    Inventors: Neal Jakel, John Kwik, Michael Franko, Andrew Whalen
  • Patent number: 11020365
    Abstract: This invention relates to a mixture of at least three fatty acids selected from palmitic acid, oleic acid, stearic acid, linoleic acid, alpha-linolenic acid, gamma-linolenic acid, eicosapentaenoic acid (EPA), docosahexaenoic acid (DHA), azelaic acid and myristic acid. This invention also relates to the use of the aforesaid mixture in the treatment of inflammatory pathologies.
    Type: Grant
    Filed: March 4, 2014
    Date of Patent: June 1, 2021
    Assignee: AGAIN LIFE ITALIA SRL
    Inventor: Lodovico Burattin
  • Patent number: 11015166
    Abstract: A method of extracting frustules from benthic pennate diatoms is disclosed. The method includes culturing benthic pennate diatoms in an industrial biofilm process, wherein in the industrial biofilm process the benthic pennate diatoms are growing on at least one surface in a water-containing compartment and wherein the benthic pennate diatoms forms a biofilm on the at least one surface; harvesting the benthic pennate diatoms from the at least one surface; and extracting the frustules by separating the frustules from organic biomass contained in the benthic pennate diatoms.
    Type: Grant
    Filed: December 5, 2018
    Date of Patent: May 25, 2021
    Assignee: SWEDISH ALGAE FACTORY AB
    Inventors: Sofie Allert, Angela Wulff
  • Patent number: 10995348
    Abstract: A method of producing lipids, comprising the steps of: culturing a transformant in which the expression of a gene encoding at least any one of the following proteins (A) to (D) is enhanced in a cyanobacteria cell, and producing fatty acids or lipids containing these fatty acids as components. (A) a protein consisting of the amino acid sequence set forth in SEQ ID NO: 1; (B) a protein consisting of an amino acid sequence having 60% or more identity with the amino acid sequence of the protein (A), and having ?-ketoacyl-ACP synthase activity; (C) a protein consisting of the amino acid sequence set forth in SEQ ID NO: 44; and (D) a protein consisting of an amino acid sequence having 60% or more identity with the amino acid sequence of the protein (C), and having ?-ketoacyl-ACP synthase activity.
    Type: Grant
    Filed: April 4, 2017
    Date of Patent: May 4, 2021
    Assignee: Kao Corporation
    Inventors: Mika Terai, Akihito Kawahara, Tatsuro Ozaki
  • Patent number: 10995351
    Abstract: Systems and methods for producing carbohydrate (e.g., sugar) streams (and recycling enzymes) from a pretreated or untreated biomass such as cellulosic feedstock, including, for example, “brown stock” feedstock, or waste or recycled fiber sludge produced in the pulp and paper industry, such as for biochemical (e.g., biofuel) production, are provided. In one example, the system and method can produce high purity C6 (glucose and/or fructose) and/or C5 (xylose) sugar streams, and other carbohydrates and/or fibrous materials, from cellulosic feedstocks, such as brown stock or waste fiber sludge, that can be effectively converted into various biochemical products, such as ethanol.
    Type: Grant
    Filed: September 14, 2020
    Date of Patent: May 4, 2021
    Assignee: Fluid Quip Technologies, LLC
    Inventors: Jeffrey P Robert, Neal Jakel, Donald M. Cannon
  • Patent number: 10988784
    Abstract: Methods for producing a bioproduct selected from acetone, isopropanol and a combination thereof with a microorganism in a fermentor are disclosed. The methods include separating cells of the microorganism from a fermentation broth to form separated cells and recycling at least a fraction of the separated cells to the fermentor to achieve one or more of the following: (1) cell concentration in said fermentor greater than 2 g/L; mass yield on a first feedstock greater than 32%; productivity greater than 0.12 g/L/h; and bioproduct titer greater than 10 g/L.
    Type: Grant
    Filed: September 30, 2016
    Date of Patent: April 27, 2021
    Assignee: WHITE DOG LABS, INC.
    Inventors: Bryan Patrick Tracy, Shawn William Jones, Daniel Knox Mitchell, Aharon M. Eyal, Karthikeyan Ramachandriya
  • Patent number: 10913960
    Abstract: A microbial cell, which is genetically modified to increase the expression relative to the corresponding genetically unmodified cell of an AlkB alkane hydroxylase (Eb) having an amino acid sequence at least 95% identical with the amino acid sequence of SEQ ID NO: 1 and a wax-ester synthase (Ef) having an amino acid sequence at least 95% identical with the amino acid sequence of SEQ ID NO: 2. The cell does not have a genetic modification that increases formation of a carboxylic acid or a carboxylate ester from a simple carbon source.
    Type: Grant
    Filed: November 23, 2016
    Date of Patent: February 9, 2021
    Assignee: Evonik Operations GmbH
    Inventors: Steffen Schaffer, Thomas Haas, Wilhelm Bruegging, Ralf Meier
  • Patent number: 10907223
    Abstract: The present invention provides for a method of producing a polysaccharide gel comprising: (a) culturing or growing a cyanobacterium or a mixture of cyanobacteria species in a medium under conditions such that the cyanobacterium or the mixture of cyanobacteria species produces a polysaccharide or a mixture of polysaccharides, (b) optionally separating the polysaccharide or the mixture of polysaccharides from the medium and/or the cyanobacterium or the mixture of cyanobacteria species, and (c) increasing the pH of the polysaccharide or the mixture of polysaccharides such that the polysaccharide or the mixture of polysaccharides forms a gel.
    Type: Grant
    Filed: April 2, 2018
    Date of Patent: February 2, 2021
    Assignees: The Regents of the University of California, HelioBioSys, Inc.
    Inventors: Mona Mirsiaghi, Eric Sundstrom, Deepti Tanjore, Todd Pray, Rocco L. Mancinelli, David T. Smernoff
  • Patent number: 10881631
    Abstract: A recombinant camelina plant or cell comprising one or more polynucleotides encoding a ?6-desaturase, a ?6-elongase and a ?5-desaturase operably linked with one or more regulatory sequences.
    Type: Grant
    Filed: April 12, 2013
    Date of Patent: January 5, 2021
    Assignee: ROTHAMSTED RESEARCH LTD.
    Inventors: Johnathan Napier, Olga Sayanova, Noemi Ruiz-Lopez, Richard Haslam
  • Patent number: 10870814
    Abstract: [Problem] Provision of a method for fractionating lipids using liquefied dimethyl ether as a solvent. [Means for Solving] To provide a method for fractionating a lipid using liquefied dimethyl ether as a solvent. [Solution] A method for fractionating a lipid, which comprises subjecting a microbial biomass to extraction using liquefied dimethyl ether as a solvent and then fractionating the lipid utilizing separation selectivity for the lipid. A method for producing a lipid, which comprises subjecting a microbial biomass to extraction using liquefied dimethyl ether as a solvent, then fractionating a portion of the lipid utilizing separation selectivity for the lipid to modify the fatty acid composition of the remainder of the lipid which remains in the microbial biomass, and then extracting the lipid having a modified fatty acid composition.
    Type: Grant
    Filed: March 30, 2015
    Date of Patent: December 22, 2020
    Assignee: NIPPON SUISAN KAISHA, LTD.
    Inventors: Hideki Kanda, Motonobu Goto, Yuji Okita, Eiji Ohashi
  • Patent number: 10864163
    Abstract: The invention relates to methods for attaching therapeutic agents to structures produced by thermally induced phase separation as well as methods for coating devices and producing multi-layered microspheres.
    Type: Grant
    Filed: December 22, 2014
    Date of Patent: December 15, 2020
    Assignee: UCL Business LTD
    Inventor: Richard Michael Day
  • Patent number: 10865421
    Abstract: Provided herein are novel acyltransferases and methods of using such novel acyltransferases in making medium-chain fatty acids.
    Type: Grant
    Filed: April 16, 2019
    Date of Patent: December 15, 2020
    Assignee: NUtech Ventures
    Inventors: Edgar Cahoon, Umidjon Iskandarov, Hae Jin Kim, Jillian Collins-Silva
  • Patent number: 10844411
    Abstract: The invention relates to a method for accumulating triacylglycerols in microalgae by inhibiting the sterol metabolism, by incubating the microalgae with an inhibitor of sterol metabolism. The invention also relates to a method for producing fatty acids, biofuels, pharmaceutical or cosmetic compositions, and also food supplements, comprising a triacylglycerols accumulation step in microalgae according to the invention. Finally, the invention concerns the use of an inhibitor of sterol metabolism to accumulate triglycerides in microorganisms, and preferably microalgae.
    Type: Grant
    Filed: January 27, 2015
    Date of Patent: November 24, 2020
    Assignees: Commissariat A L'Energie Atomique Et Aux Energies Alternatives, Centre National De La Recherche Scientifique
    Inventors: Melissa Conte, Lina-Juana Dolch, Coline Mei, Caroline Barette, Dimitris Petroutsos, Denis Falconet, Juliette Jouhet, Fabrice Rebeille, Jean-Christophe Cintrat, Eric Marechal
  • Patent number: 10844317
    Abstract: The method of producing a lipid composition comprises an extraction treatment step to extract a lipid comprised in a hydrated raw material by using an extraction solvent comprising a polar solvent and a nonpolar solvent, and a separation treatment step to separate an extract solution obtained in the extraction treatment step into a polar solvent phase comprising a first lipid fraction and a nonpolar solvent phase comprising a second lipid fraction.
    Type: Grant
    Filed: February 12, 2019
    Date of Patent: November 24, 2020
    Assignee: IHI CORPORATION
    Inventors: Junichi Okuyama, Tomomi Yajima, Koretaro Takahashi, Takeya Yoshioka
  • Patent number: 10821090
    Abstract: The present invention refers to new purified compositions of long chain polyunsaturated fatty acids, or their salts or esters, characterized by being essentially free from other usually present—but structurally different—components, such as furan fatty acids, phytanic and pristanic acids, squalene, and some oligomers, as well as several “persistent” environmental pollutants, such as polychlorinated dibenzo-dioxins and polychlorinated dibenzo-furans, polychlorinated biphenyls, polybrominated diphenyl-ethers, polycyclic aromatic hydrocarbons, and others, which are also usually present and extremely toxic. The invention also refers to the purification method to obtain said compositions and the use thereof as food, food for special medical use, food and diet supplement, and drug.
    Type: Grant
    Filed: March 22, 2016
    Date of Patent: November 3, 2020
    Inventor: Tiberio Bruzzese
  • Patent number: 10821411
    Abstract: A catalytic reactor comprises a filter unit which extracts and collects particles from the fluid flow stream above the reactor internals, the filter unit comprises elements which are safely, easily and quickly handled without the need for tools.
    Type: Grant
    Filed: July 3, 2018
    Date of Patent: November 3, 2020
    Assignee: Haldor Topsøe A/S
    Inventors: Klaus Risbjerg Jarlkov, Emir Zahirovic
  • Patent number: 10815506
    Abstract: A method for producing fatty acid alkyl esters, comprising providing a system comprising an oil phase/hydrophobic phase an a hydrophilic phase, and reacting a fatty acid feedstock present in said oil phase/hydrophobic phase with alcohol in the presence of water and one or more lipolytic enzymes.
    Type: Grant
    Filed: May 28, 2015
    Date of Patent: October 27, 2020
    Assignee: Novozymes A/S
    Inventors: Anders Rancke-Madsen, Per Munk Nielsen
  • Patent number: 10798952
    Abstract: The present invention is directed to isolated microorganisms as well as strains and mutants thereof, biomasses, microbial oils, compositions, and cultures; methods of producing the microbial oils, biomasses, and mutants; and methods of using the isolated microorganisms, biomasses, and microbial oils.
    Type: Grant
    Filed: February 17, 2017
    Date of Patent: October 13, 2020
    Assignee: DSM IP Assets B.V.
    Inventors: Kirk E. Apt, Paul Warren Behrens, Jon Milton Hansen, Joseph W. Pfeifer, III, Tracey Lynn Stahl, Ross Zirkle
  • Patent number: 10787687
    Abstract: The present disclosure relates to a method for obtaining sugar and lignin fractions from lignocellulosic materials and to a method for producing fermentation products using the sugars obtained from the lignocellulose. The present disclosure also relates to a method for improving the sugar yield in the enzymatic hydrolysate by introducing a pre-treatment step of hydrothermal hydrolysis with a delignification step using a cationic compound.
    Type: Grant
    Filed: December 11, 2014
    Date of Patent: September 29, 2020
    Assignee: NESTE OYJ
    Inventors: Heidi Vainio, Olavi Myllymaki, Ville Pihlajaniemi, Mika Sipponen, Simo Laakso, Ilkka Lehtomaki, Ossi Pastinen, Perttu Koskinen
  • Patent number: 10787648
    Abstract: The disclosure relates to omega-hydroxylase-related fusion polypeptides that result in improved omega-hydroxylated fatty acid derivative production when expressed in recombinant host cells. The disclosure further relates to microorganisms for expressing the omega-hydroxylase-related fusion polypeptides for the production of omega-hydroxylated fatty acid derivatives.
    Type: Grant
    Filed: December 13, 2016
    Date of Patent: September 29, 2020
    Assignee: GENOMATICA, INC.
    Inventors: Andreas Schirmer, Baolong Zhu, Emanuela Popova
  • Patent number: 10731169
    Abstract: The present invention relates to transgenic nucleic acids, expression cassettes, vectors, plant cells, plant organs and plants. The invention also relates to methods for increasing expression or activity of a target gene, particularly in a plant cell or plant organ, and also to uses of recombinant nucleic acids and expression cassettes to increase expression or activity of a target gene or for manufacturing of a vector, plant cell, plant organ or plant. Incidentally, the invention relates to enhancers for achieving increased expression or activity of a target gene, particularly in a plant cell or plant organ, when operably linked to a promoter functional in such plant cell, plant organ or plant. The invention is described herein with reference to the technical field of production of polyunsaturated fatty acids (PUFAs), without being limited to this technical field. For the production of desired molecules in plant cells, e.g.
    Type: Grant
    Filed: July 3, 2014
    Date of Patent: August 4, 2020
    Assignee: BASF PLANT SCIENCE COMPANY GMBH
    Inventors: Toralf Senger, Joerg Bauer
  • Patent number: 10696924
    Abstract: PROBLEM To provide a composition comprising highly enriched PUFA or its alkyl esters while containing fatty acid esters of 3-MCPD at adequately low concentrations and to provide an efficient method for producing the composition. MEANS FOR SOLVING A composition that contains fatty acids or fatty acid alkyl esters as its major component, the composition containing highly unsaturated fatty acid or alkyl ester thereof, wherein the proportion of the highly unsaturated fatty acid in the constituent fatty acids of the composition is 50 area % or more and wherein the concentration of 3-MCPD as found upon analyzing the composition by American Oil Chemists' Society official method Cd 29b-13 assay A is less than 1.80 ppm.
    Type: Grant
    Filed: May 6, 2019
    Date of Patent: June 30, 2020
    Assignee: Nippon Suisan Kaisha, Ltd.
    Inventors: Nobushige Doisaki, Kiyomi Furihata, Hideaki Yamaguchi
  • Patent number: 10689607
    Abstract: Disclosed are an enzymatic batchwise or continuous process for the production of fatty acid alkyl esters for use in the biofuels, food and detergent industries and a system therefor. The process utilizes enzymes immobilized on a hydrophobic resin mixed with a fatty acid source and an alcohol or alcohol donor in the presence of an alkaline or mild alkaline aqueous buffer, or in the presence of water or aqueous solution. The production process for fatty acid alkyl esters is carried out by transesterification or esterification simultaneously or sequentially. The biocatalyst activity is maintained with no significant activity losses in multiple uses and also avoids the accumulation of glycerol and water by-products or other hydrophilic compounds on the biocatalyst.
    Type: Grant
    Filed: May 21, 2018
    Date of Patent: June 23, 2020
    Assignee: Trans Bio-Diesel Ltd.
    Inventors: Sobhi Basheer, Maisa Haj, Usama Mohsen, Doaa Shehadeh, Ahmad Hindawi, Emad Masoud, Ahmad Egbarieh, Ramez Masri
  • Patent number: 10662443
    Abstract: A method for producing alkane and a recombinant microorganism with good alkane productivity in a reaction system for synthesizing alkane by alkane synthase activity are provided. Alkane productivity is significantly improved in a system for synthesizing alkane by alkane synthase in the presence of ferredoxin. Specifically, the method for producing alkane according to the present invention comprises synthesizing alkane by alkane synthase in the presence of ferredoxin.
    Type: Grant
    Filed: August 15, 2011
    Date of Patent: May 26, 2020
    Assignee: TOYOTA JIDOSHA KABUSHIKI KAISHA
    Inventor: Masaaki Mori
  • Patent number: 10640728
    Abstract: The invention discloses a method for preparing rapeseed oil by a semi-solid aqueous enzymatic process, belonging to the field of functional foods and health care products. The present invention first prepares a semi-solid rapeseed paste with 3.5-4.5% moisture content, which is hydrolyzed by a mixture of pectinase, cellulase and alkaline protease to extract rapeseed oil. The resulting rapeseed oil contains high levels of active ingredients including totaxin, sterol, phenols and beta-carotene. The rapeseed oil of the invention can be added into animal feeds, which helps to reduce animal blood lipid levels and body weight. It can significantly reduce the contents of total triglyceride, total cholesterol and LDC-C in the blood, and, at the same time, increases the level of HDL-C in the blood. In addition, the rapeseed oil prepared by the present invention can relieve hepatic steatosis in hyperlipemia rats.
    Type: Grant
    Filed: May 15, 2018
    Date of Patent: May 5, 2020
    Assignee: Jiangnan University
    Inventors: Yuanfa Liu, Peirang Cao, Jinwei Li, Liang Zhang
  • Patent number: 10632047
    Abstract: Nutritional formulas comprising long-chain polyunsaturated fatty acids (LC-PUFAs) are provided, along with methods and devices for preparing and/or administering nutritional formulas. In some embodiments, a percentage of the LC-PUFAs in the nutritional formula are in the form of monoglycerides and/or free fatty acids. In some embodiments, the nutritional formulas do not comprise added lipase. Also provided are methods for providing nutrition to a subject, methods for improving fat absorption, methods for improving cognitive ability, methods for preventing chronic lung disease, and methods for reducing the length of time a patient requires total parenteral nutrition.
    Type: Grant
    Filed: August 23, 2017
    Date of Patent: April 28, 2020
    Assignee: Alcresta Therapeutics, Inc.
    Inventors: Alexey L. Margolin, Robert Gallotto, Bhami Shenoy