Lactic Acid Patents (Class 435/139)
  • Patent number: 10214754
    Abstract: Provided is a transformant which can produce lactic acid with a high productivity without requiring neutralization with an alkali and is excellent in both of lactic acid production capability and growth ability and its production process, and a method for producing lactic acid by using the transformant. A transformant comprising 3 copies of a human lactate dehydrogenase gene that is introduced into a Schizosaccharomyces pombe host, wherein a gene encoding pyruvate decarboxylase 2 of the Schizosaccharomyces pombe host is deleted or inactivated.
    Type: Grant
    Filed: October 6, 2015
    Date of Patent: February 26, 2019
    Assignee: JMTC Enzyme Corporation
    Inventors: Futoshi Hara, Ayako Kashima, Shuichiro Kimura
  • Patent number: 10190076
    Abstract: The present invention relates generally to corn dry-milling, and more specifically, to methods for producing a high protein corn meal from a whole stillage byproduct produced in a corn dry-milling process for making ethanol and a system therefore. In one embodiment, a method for producing a high protein corn meal from a whole stillage byproduct includes, in a corn dry-milling process for making ethanol, separating the whole stillage byproduct into an insoluble solids portion and a thin stillage portion. The thin stillage portion is separated into a protein portion and a water soluble solids portion. Next, the protein portion is dewatered then dried to define a high protein corn meal that includes at least 40 wt % protein on a dry basis.
    Type: Grant
    Filed: July 1, 2015
    Date of Patent: January 29, 2019
    Assignee: Fluid Quip, Inc.
    Inventor: Chie Ying Lee
  • Patent number: 10190769
    Abstract: The invention provides a process for the conversion of biomass into a biomass product which is suitable for use as a fuel. The biomass is of plant origin and comprises microorganisms naturally occurring in the biomass. The process comprises—preparing a slurry by dispersing the biomass comprising the naturally occurring microorganisms in an aqueous liquid, maintaining the slurry at conditions suitable for aerobic digestion by the microorganisms to obtain a slurry comprising the biomass product as a dispersed solid phase, and—recovering the biomass product. The recovering comprises washing and drying the biomass product. The invention also provides a combustion process.
    Type: Grant
    Filed: August 11, 2011
    Date of Patent: January 29, 2019
    Assignee: NEWFOSS HOLDING B.V.
    Inventors: Dirk Jan Vos, Simon Rustenburg
  • Patent number: 10160932
    Abstract: The present invention relates generally to corn dry-milling, and more specifically, to methods for producing a high protein corn meal from a whole stillage byproduct produced in a corn dry-milling process for making ethanol and a system therefore. In one embodiment, a method for producing a high protein corn meal from a whole stillage byproduct includes, in a corn dry-milling process for making ethanol, separating the whole stillage byproduct into an insoluble solids portion and a thin stillage portion. The thin stillage portion is separated into a protein portion and a water soluble solids portion. Next, the protein portion is dewatered then dried to define a high protein corn meal that includes at least 40 wt % protein on a dry basis.
    Type: Grant
    Filed: February 26, 2015
    Date of Patent: December 25, 2018
    Assignee: Fluid Quip, Inc.
    Inventor: Chie Ying Lee
  • Patent number: 10155967
    Abstract: The present invention relates to Saccharomyces sp. capable of producing lactic acid with a decreased activity of pyruvate decarboxylase (PDC) and increased activities of aldehyde dehydrogenase (ALD) and acetyl-CoA synthetase (ACS), and a method of producing lactic acid from the culture medium obtained by culturing the microorganism.
    Type: Grant
    Filed: June 12, 2015
    Date of Patent: December 18, 2018
    Assignee: CJ CHEILJEDANG CORPORATION
    Inventors: Eun Bin Yang, Tae Hee Lee, Seon Hye Kim, Gyuhyeon Song, Cheol Woong Ha, Kyungsu Na, Young Lyeol Yang, Min Sun Kang, Hyo Hyoung Lee
  • Patent number: 10072117
    Abstract: A method of producing lactic acid includes removing glycerol from an aqueous lactic acid solution containing glycerol as an impurity using an ion-exchange resin. The lactic acid can be separated simply and at low cost from an aqueous lactic acid solution containing glycerol as an impurity.
    Type: Grant
    Filed: May 21, 2013
    Date of Patent: September 11, 2018
    Assignee: Toray Industries, Inc.
    Inventors: Kenji Kawamura, Masateru Ito, Katsushige Yamada
  • Patent number: 10000537
    Abstract: The invention relates to a polypeptide having one or more substitution at a position corresponding to position 339 or 376 of SEQ ID NO: 59, wherein the polypeptide is a member of the Major Facilitator Superfamily (MFS). In an embodiment, the substitution is at position corresponding to 376 and wherein the amino acid at that position is replaced by an amino acid that has a van der Waals volume of 80 to 138 ?3 and a side chain hydrophobicity of 10 to 100 ?tR.
    Type: Grant
    Filed: June 4, 2014
    Date of Patent: June 19, 2018
    Assignee: DSM IP ASSETS B.V.
    Inventors: Paul Klaassen, Paulus Petrus De Waal, Rene Marcel De Jong, Arnold Jacob Mattieu Driessen, Jeroen Gerben Nijland, Hyun Yong Shin
  • Patent number: 9994877
    Abstract: Provided are a genetically engineered yeast cell having increased activity of SUL1, STR3, HXT7, ERR1, GRX8, MXR1, GRE1, MRK1, AAD10 or a combination thereof, compared to a parent cell, and also having acid tolerance, a method of preparing the same, and a method of producing lactate using the same.
    Type: Grant
    Filed: May 12, 2016
    Date of Patent: June 12, 2018
    Assignee: SAMSUNG ELECTRONICS CO., LTD.
    Inventors: Soonchun Chung, Jinha Kim, Kwangmyung Cho
  • Patent number: 9961914
    Abstract: The present invention discloses a method to prepare a fruit ferment containing propionic acid and/or a salt thereof comprising i) obtaining a liquid fruit preparation, ii) optionally supplementing the liquid fruit preparation with water and/or with additional components to support fermentation, iii) fermenting the optionally supplemented liquid fruit preparation with a propionic acid-producing bacterial strain, iv) optionally further processing the fermentation product, to obtain the fruit ferment. Next to a propionic acid-producing bacterial strain, a lactic acid-producing bacterial strain may be used in the fermentation. The invention also relates to a fruit ferment obtainable by said method and the use in a food, beverage, as a probiotic and to improve the sensory properties and/or the storage stability is also disclosed.
    Type: Grant
    Filed: December 7, 2011
    Date of Patent: May 8, 2018
    Assignee: PURAC BIOCHEM BV
    Inventors: Arne Olav Sliekers, Eelco Anthonius Johannes Heintz
  • Patent number: 9949486
    Abstract: The invention relates to an agrochemical composition containing a compound of formula (1), a glutamic acid compound, and a source of magnesium and/or a source of iron. The composition applied e.g. foliarly has demonstrated synergistic benefits that improve crop yields and quality characteristics.
    Type: Grant
    Filed: January 14, 2015
    Date of Patent: April 24, 2018
    Assignee: Crop Intellect Ltd.
    Inventor: Apostolos Papadopoulos
  • Patent number: 9885067
    Abstract: A recombinant yeast cell having a decreased RGT1 protein activity and an increased ability to produce a glycolytic intermediate or a glycolytic intermediate-derived substance, compared to a parent cell; methods of producing the same; and methods of producing the glycolytic intermediate or the glycolytic intermediate-derived substance using the same.
    Type: Grant
    Filed: December 28, 2015
    Date of Patent: February 6, 2018
    Assignee: SAMSUNG ELECTRONICS CO., LTD.
    Inventors: Jinsuk Lee, Changduk Kang, Seunghyun Lee, Kwangmyung Cho
  • Patent number: 9796896
    Abstract: Fertilizers and fertilizer additive compositions and methods are provided. The compositions and methods comprise utilizing by-products from the manufacture of biodiesel and/or fatty alkyl esters, wherein the by-product comprises glycerin, mong, moisture, C6-C24 saturated and unsaturated fatty acids, C6-C24 saturated and unsaturated fatty acid salts, methyl esters, ethyl esters and combinations thereof.
    Type: Grant
    Filed: December 21, 2012
    Date of Patent: October 24, 2017
    Inventors: Joan Lynch, Bo Tran
  • Patent number: 9745560
    Abstract: The present invention provides a multi-component enzyme system that hydrolyzes hemicellulose oligomers from hardwood which can be expressed, for example, in yeast such as Saccharomyces cerevisiae. In some embodiments, this invention provides for the engineering of a series of biocatalysts combining the expression and secretion of components of this enzymatic system with robust, rapid xylose utilization, and ethanol fermentation under industrially relevant process conditions for consolidated bioprocessing. In some embodiments, the invention utilizes co-cultures of strains that can achieve significantly improved performance due to the incorporation of additional enzymes in the fermentation system.
    Type: Grant
    Filed: March 15, 2013
    Date of Patent: August 29, 2017
    Assignee: Lallemand Hungary Liquidity Management LLC
    Inventors: John E. McBride, Erin Wiswall, Indraneel Shikhare, Haowen Xu, Naomi Thorngren, Heidi H. Hau, Emily Stonehouse
  • Patent number: 9677098
    Abstract: A yeast cell capable of producing lactate, a method of preparing the yeast cell, and a method of producing lactate by using the yeast cell, wherein theyeast cell has a reduced activity of rim15 protein, igo2 protein, or a combination thereof, and an increased activity of an enzyme that catalyzes conversion from pyruvate to lactate, compared to a parent cell.
    Type: Grant
    Filed: July 24, 2015
    Date of Patent: June 13, 2017
    Assignee: SAMSUNG ELECTRONICS CO., LTD.
    Inventors: Juyoung Lee, Changduk Kang, Jiyoon Song, Seunghyun Lee, Jinhwan Park, Kwangmyung Cho
  • Patent number: 9604895
    Abstract: A process for producing lactic acid is provided. The process comprises (a) reacting a stream rich in saccharide with sodium hydroxide in the presence of water to produce a reaction mixture comprising sodium lactate; (b) reacting at least a portion of the sodium lactate with HCl to produce lactic acid and sodium chloride; (c) converting at least a portion of the sodium chloride to chlorine and sodium hydroxide; and (d) recycling at least a portion of the sodium hydroxide produced in step (c) to step (a). Also provided are processes for the production of alkyl lactate, oligomeric lactic acid, lactide, alkyl lactyllactate, poly-lactic acid, propylene glycol and acrylic acid.
    Type: Grant
    Filed: September 5, 2014
    Date of Patent: March 28, 2017
    Assignee: Plaxica Limited
    Inventors: Stephen Donegan, Philip James Goodier, Edward Leslie Marshall, Urvish Rameshchandra Pandya, Ajitkumar Mukundrai Patel
  • Patent number: 9598709
    Abstract: Provided is a yeast cell having a stress tolerance, wherein the yeast cell has enhanced MSN2 activity, a method of producing the yeast cell, and a method of producing lactate by using the same.
    Type: Grant
    Filed: July 28, 2015
    Date of Patent: March 21, 2017
    Assignee: SAMSUNG ELECTRONICS CO., LTD.
    Inventors: Sungsoo Kim, Sunghaeng Lee, Dongsik Yang, Huisub Lim, Kwangmyung Cho
  • Patent number: 9562243
    Abstract: A yeast cell with reduced pyruvate to oxaloacetate conversion activity, and a method of producing lactate using the yeast cell.
    Type: Grant
    Filed: December 8, 2014
    Date of Patent: February 7, 2017
    Assignee: SAMSUNG ELECTRONICS CO., LTD.
    Inventors: Youngkyoung Park, Changduk Kang, Jiyoon Song, Juyoung Lee, Seunghyun Lee, Kwangmyung Cho
  • Patent number: 9528132
    Abstract: The present invention relates to a polynucleotide encoding a recombinant scaffolding polypeptide comprising at least a signal peptide, a Cellulose Binding Domain, two cohesin domains and an S-layer Homology domain, wherein said isolated polynucleotide preferably comprises all or an active part of the nucleotide sequence as set forth in SEQ ID NO:2. The present invention further relates to vectors comprising such polynucleotides, recombinant lactic acid bacteria, and method for degrading a cellulosic biomass using such recombinant lactic acid bacteria.
    Type: Grant
    Filed: June 3, 2013
    Date of Patent: December 27, 2016
    Assignee: CARBIOS
    Inventors: Roberto Mazzoli, Enrica Pessione, Loredana Tarraran, Chiara Gandini
  • Patent number: 9499822
    Abstract: A simple and inexpensive method for selectively culturing a microorganism which method makes it possible to selectively culture a microorganism of interest even without using a sterilization operation or an antibiotic substance is provided. The method according to the present invention selectively culturing a microorganism includes the step of culturing, in a culture medium containing phosphorous acid as a sole phosphorous source, a recombinant microorganism into which a phosphite dehydrogenase gene has been introduced.
    Type: Grant
    Filed: August 8, 2013
    Date of Patent: November 22, 2016
    Assignee: HIROSHIMA UNIVERSITY
    Inventors: Akio Kuroda, Ryuichi Hirota, Takenori Ishida
  • Patent number: 9428773
    Abstract: The present disclosure provides recombinant cyanobacteria having acetolactate synthase activity and acetolactate decarboxylase activity, as well as recombinant cyanobacteria having acetolactate synthase activity, acetolactate decarboxylase activity, and secondary alcohol dehydrogenase activity. Methods for the production of the recombinant cyanobacteria, as well as methods for use thereof for production of acetoin and 2,3-butanediol from carbon dioxide and light are also provided.
    Type: Grant
    Filed: September 27, 2013
    Date of Patent: August 30, 2016
    Assignee: The Regents of the University of California
    Inventors: Shota Atsumi, John W. K. Oliver, Iara M. P. Machado
  • Patent number: 9428777
    Abstract: Objects of the present invention are to provide a transformant which can produce lactic acid with high productivity without requiring neutralization with an alkali, a method for producing the same, and a method for producing lactic acid by using the transformant. Namely, they are a transformant, in which Schizosaccharomyces pombe is used as a host, a lactate dehydrogenase gene of Lactobacillus pentosus is introduced, and a part of a gene cluster encoding a pyruvate decarboxylase in the Schizosaccharomyces pombe host is deleted or inactivated; a method for producing the transformant; and a method for producing lactic acid by using the transformant.
    Type: Grant
    Filed: February 20, 2015
    Date of Patent: August 30, 2016
    Assignee: ASAHI GLASS COMPANY, LIMITED
    Inventors: Futoshi Hara, Shuichiro Kimura
  • Patent number: 9416380
    Abstract: A yeast cell comprising LDH from a Sordaria genus fungi, in which activity of lactate dehydrogenase converting pyruvate into lactate is increased, as well as a method of preparing the yeast cell and a method of using the yeast cell to produce lactate.
    Type: Grant
    Filed: July 24, 2014
    Date of Patent: August 16, 2016
    Assignee: SAMSUNG ELECTRONICS CO., LTD.
    Inventors: Sung-soo Kim, So-young Lee, Chang-duk Kang, Ju-young Lee, Kwang-myung Cho
  • Patent number: 9416222
    Abstract: Disclosed is a test and test apparatus used to determine whether lactic acid is suitable for polymerization. The test apparatus comprises a) means for the polycondensation of the lactic acid to form a pre-polymer, b) means for depolymerization of the prepolymer to form dilactide, and c) means for carrying out analytical methods to determine at least one of the dilactide yield and/or the racemization, wherein lactic acid which is suitable for polymerization exhibits a dilactide yield of >90% and a racemization of <5%.
    Type: Grant
    Filed: October 22, 2012
    Date of Patent: August 16, 2016
    Assignees: THYSSENKRUPP INDUSTRIAL SOLUTIONS AG, UHDE INVENTA-FISCHER GMBH
    Inventors: Joachim Schulze, Rainer Hagen, Wolfgang Tietz, Shashank Ghanegaonkar
  • Patent number: 9365869
    Abstract: A process for increasing a production of alcohol by fermenting sugars may include applying a direct current electrical field to a fermentation broth, which occurs before the fermentation broth inoculates with a concentration of yeasts; and controlling a pH value of said fermentation broth to maintain the pH value with a specific range.
    Type: Grant
    Filed: July 24, 2013
    Date of Patent: June 14, 2016
    Assignee: ADVEL TECNOLOGIA E COMERCIA LTDA.
    Inventor: José Francisco Lopes
  • Patent number: 9346843
    Abstract: The present invention relates to a process for generating glucose and glucose derivatives from the direct contacting of cellulose, hemicelluloses and/or polysaccharides with a mixed super critical fluid system of alcohol and water whereby the partial pressure of the system provides for both alcoholysis and hydrolysis of the material to generate primarily glucose, and glucose derivatives.
    Type: Grant
    Filed: November 1, 2011
    Date of Patent: May 24, 2016
    Assignee: Inventure Renewables, Inc.
    Inventors: William Rusty Sutterlin, Mark G. Tegen, William Berry
  • Patent number: 9340807
    Abstract: A polypeptide conferring an acid-tolerant property on a yeast cell, a polynucleotide encoding the polypeptide, a yeast cell including an increased amount of the polypeptide, a method of producing a product by using the yeast cell, and a method of producing an acid-tolerant yeast cell are provided.
    Type: Grant
    Filed: September 2, 2014
    Date of Patent: May 17, 2016
    Assignee: SAMSUNG ELECTRONICS CO., LTD.
    Inventors: Woo Yong Lee, Chang Duk Kang, Ju Young Lee, Kwang Myung Cho
  • Patent number: 9315834
    Abstract: Provided is a method for producing xylonic acid from xylose with a recombinant fungal strain that is genetically modified to express a xylose dehydrogenase gene, which is able to convert xylose to xylonolactone, which is spontaneously or enzymatically hydrolyzed to xylonic acid. The xylonic acid is excreted outside the host cell. Xylonate production may be coupled with xylitol production. Alternatively, if xylitol production is not desired, its production is reduced by removing the aldose reductase (or specific xylose reductase) enzyme, which converts xylose to xylitol. Expression of a heterologous lactonase encoding gene may result in higher acid concentrations. The method is suitable for producing xylonic acid from a hemicellulose hydrolysate such as hydrolyzed lignocellulosic plant biomass.
    Type: Grant
    Filed: March 17, 2010
    Date of Patent: April 19, 2016
    Assignee: Teknologian Tutkimuskeskus VTT
    Inventors: Peter Richard, Marilyn Wiebe, Mervi Toivari, Dominik Mojzita, Laura Ruohonen, Merja Penttilä
  • Patent number: 9294498
    Abstract: Determining an entity's cybersecurity risk and benchmarking that risk includes non-intrusively collecting one or more types of data associated with an entity. Embodiments further include calculating a security score for at least one of the one or more types of data based, at least in part, on processing of security information extracted from the at least one type of data, wherein the security information is indicative of a level of cybersecurity. Some embodiments also comprise assigning a weight to the calculated security score based on a correlation between the extracted security information and an overall security risk determined from analysis of one or more previously-breached entities in the same industry as the entity. Additional embodiments include calculating an overall cybersecurity risk score for the entity based, at least in part, on the calculated security score and the weight assigned to the calculated security score.
    Type: Grant
    Filed: May 1, 2015
    Date of Patent: March 22, 2016
    Assignee: SecurityScorecard, Inc.
    Inventors: Aleksandr Yampolskiy, Rob Blackin, Alexander Heid, Samuel Kassoumeh
  • Patent number: 9284519
    Abstract: Provided herein is a transgenic bacteria engineered to accumulate carbohydrates, for example disaccharides. Also provided is a photobioreactor for cultivating photosynthetic microorganisms comprising a non-gelatinous, solid cultivation support suitable for providing nutrients and moisture to photosynthetic microorganisms and a physical barrier covering at least a portion of the surface of the cultivation support. Devices for the large scale and continuous cultivation of photosynthetic microorganisms incorporating photobioreactors and methods of use are disclosed. Also disclosed are methods of producing fermentable sugar from photosynthetic microorganisms using a photobioreactor of the invention.
    Type: Grant
    Filed: May 7, 2014
    Date of Patent: March 15, 2016
    Assignee: Proterro, Inc.
    Inventors: John Aikens, Robert J. Turner
  • Patent number: 9238618
    Abstract: Processes for the production of polyols from sources such as dried distillers grains plus solubles (DDGS) make use of a two-stage reaction scheme. In the first stage, the proteinaceous starting material is reacted with an aminating agent, such as diethanolamine (DEOA), to generate amino-amides and amides. These products are then reacted with an alkoxylating agent, preferably a substituted or unsubstituted epoxide to yield polyols. These polyols may be further reacted with isocyanates to give low-cost rigid polyurethane foams. In alternate forms, lignin may be directly converted to polyols by reaction with an alkoxylating agent, optionally followed by reaction with an isocyanate to produce polyurethanes.
    Type: Grant
    Filed: October 16, 2014
    Date of Patent: January 19, 2016
    Assignees: MGPI Processing, Inc., Pittsburg State University
    Inventors: Sukh Bassi, Michael Douglas Parker, Mihail Ionescu, Zoran Petrovic
  • Patent number: 9228167
    Abstract: In a method for controlling the growth of microbes grown in a medium to high cell-densities by the fed-batch technology, a two-phase system, having a liquid phase (cultivation medium) and a solid or gel phase, the solid or gel phase provides a source of substrate-delivering polymer which is turned by an enzyme in a controlled way into a growth-limiting substrate or pH adjusting agent. Also described is a method for restricting the synthesis of growth-limiting metabolites and preventing oxygen-depletion in microbial cell cultures.
    Type: Grant
    Filed: November 29, 2007
    Date of Patent: January 5, 2016
    Assignee: OULUN YLIOPISTO
    Inventors: Antti Vasala, Peter Neubauer, Johanna Panula-Perala
  • Patent number: 9200297
    Abstract: An isolated biologically pure culture of Clostridium tyrobutyricum ITRI04001 or an isolated biologically pure culture of Clostridium tyrobutyricum having the genotypic characteristics of ITRI04001 useful for syngas fermentation. Volatile free acids are produced by a method comprising culturing a microorganism having the genotypic characteristics of ITRI04001 in a medium; providing at least one substrate comprising at least one carbon source chosen from CO and CO2 to the microorganism; and recovering at least one free volatile free acid. Syngas can be the at least one substrate in these processes for producing volatile free acids.
    Type: Grant
    Filed: October 2, 2013
    Date of Patent: December 1, 2015
    Assignee: GREEN CELLULOSITY CORPORATION
    Inventors: Chang-Chieh Chen, Yi-Te Chou, Shao-Wen Wu, Shi-Chan Tseng, Hsin-Tzu Kuo, Wan-Ting Ma
  • Patent number: 9120061
    Abstract: A process for a stream of substances containing at least one valuable substance including (A) amino acids, (B) carboxylic acids, and (C) inorganic salts includes: (1) treating the stream by nanofiltration to obtain a retentate enriched with valuable substance (A); (2) treating the permeate of step (1) by electrodialysis in order to obtain a concentrate enriched with valuable substance (C); (3) treating the diluate of step (2) using a system (3) of two stages (4) and (5) which are directly or indirectly interconnected, wherein (4) one treatment is performed by reverse osmosis and (5) one treatment is performed by electrodialysis, whereby a concentrate enriched with valuable substance (B) is obtained, (6) at least a portion of the retentate of step (4) is directly or indirectly supplied to step (5) and (7) at least a portion of the diluate of step (5) is directly or indirectly supplied to step (4).
    Type: Grant
    Filed: December 19, 2007
    Date of Patent: September 1, 2015
    Assignee: GRUENE-BIORAFFINERIE.AT GMBH
    Inventors: Werner Koschuh, Stefan Kromus
  • Patent number: 9096874
    Abstract: Provided is a method for producing lactic acid, which includes: obtaining D-lactic acid or L-lactic acid by carrying out lactic acid fermentation using a lactic acid-producing microorganism under a pressurized condition that exceeds normal pressure and is capable of maintaining lactic acid production activity of the lactic acid-producing microorganism.
    Type: Grant
    Filed: July 28, 2010
    Date of Patent: August 4, 2015
    Assignee: MITSUI CHEMICALS, INC.
    Inventor: Hiroko Shibamoto
  • Patent number: 9085783
    Abstract: A process of producing L-lactate by feeding carbon dioxide to a culture of a cyanobacterial cell and subjecting this culture to light, wherein the cell is capable of expressing a nucleic acid molecule, which upon expression confers on the cell the ability to convert a glycolytic intermediate into L-lactate and wherein aforementioned nucleic acid molecule is under the control of a regulatory system which responds to light or to a change in the concentration of a nutrient in the culture.
    Type: Grant
    Filed: April 28, 2010
    Date of Patent: July 21, 2015
    Assignee: PHOTANOL BV
    Inventors: Martijn Bekker, Maarten Joost Teixeira de Mattos, Klaas Jan Hellingwerf
  • Patent number: 9062330
    Abstract: A non-naturally occurring eukaryotic or prokaryotic organism includes one or more gene disruptions occurring in genes encoding enzymes imparting increased fumarate, malate or acrylate production in the organism when the gene disruption reduces an activity of the enzyme. The one or more gene disruptions confers increased production of acrylate onto the organism. Organisms that produce acrylate have an acrylate pathway that at least one exogenous nucleic acid encoding an acrylate pathway enzyme expressed in a sufficient amount to produce acrylate, the acrylate pathway comprising a decarboxylase. Methods of producing fumarate, malate or acrylate include culturing these organisms.
    Type: Grant
    Filed: February 13, 2012
    Date of Patent: June 23, 2015
    Assignee: Genomatica, Inc.
    Inventors: Mark J. Burk, Anthony P. Burgard, Priti Pharkya
  • Publication number: 20150147787
    Abstract: A method produces a sugar liquid from a cellulosic biomass material, which sugar liquid has improved fermentability. The method produces a sugar liquid by: adding an alkali(s) to a concentrated cellulosic biomass sugar liquid to adjust the pH to not less than 7 to precipitate an insoluble substance(s) containing at least magnesium; and performing filtration through a microfiltration membrane to remove the insoluble substance(s), to obtain a sugar liquid as a permeate.
    Type: Application
    Filed: June 4, 2013
    Publication date: May 28, 2015
    Inventors: Hiroyuki Kurihara, Katsushige Yamada
  • Publication number: 20150147786
    Abstract: A process for mechanical destructuring of starch-based biomass was developed that makes use of a short application of high compression, impact, and shearing forces. The biomass may be destructured using a specific energy input that is less than 40% of the total combustible energy of the biomass. The destructured starch-based biomass, with or without saccharification and/or in-feed glycosyl hydrolase enzymes, may be used in feed applications. The destructured starch-based may saccharified to produce syrups and fermentable sugars, and for production of products including ethanol using a biocatalyst.
    Type: Application
    Filed: November 18, 2014
    Publication date: May 28, 2015
    Inventors: KATHLEEN A CLARKSON, F Glenn Gallagher, Aaron Perelman, Luis Fernando Romero Millan, Vivek Sharma, Jayarama K. Shetty, Daniel A. Slanac, Paula Johanna Maria Teunissen
  • Publication number: 20150140626
    Abstract: Provided is a genetically engineered yeast cell with lactate production capacity, including an enzyme that catalyzes conversion of acetaldehyde to acetyl-CoA and an enzyme that catalyzes conversion of pyruvate to lactate, which activities are increased compared to a parent cell of the yeast cell, as well as a method of producing the genetically engineered yeast cell and method of producing lactate using the genetically engineered yeast cell.
    Type: Application
    Filed: November 14, 2014
    Publication date: May 21, 2015
    Inventors: Jiyoon SONG, Changduk KANG, Joonsong PARK, Sungsoo KIM, Youngkyoung PARK, Sunghaeng LEE, Soyoung LEE, Juyoung LEE, Kwangmyung CHO, Wooyong LEE
  • Publication number: 20150140606
    Abstract: A method of producing a sugar liquid from cellulose-containing biomass includes (1) to (4): (1) subjecting a cellulose-containing biomass to a dilute sulfuric acid treatment and thereafter separating the treated cellulose-containing biomass into a dilute sulfuric acid-treated liquid and a cellulose-containing solid content; (2) adding a cellulase to the cellulose-containing solid content to hydrolyze the cellulose and thereafter obtaining a sugar liquid; (3) filtering the dilute sulfuric acid-treated liquid through a nanofiltration membrane at pH 2.5 or lower to thereby separate a sugar concentrated liquid as a retentate and at the same time recover a sulfuric acid aqueous solution as a permeate; and (4) reusing the whole amount or a part of the sulfuric acid aqueous solution obtained in (3) in the dilute sulfuric acid treatment in (1).
    Type: Application
    Filed: June 11, 2013
    Publication date: May 21, 2015
    Inventors: Atsushi Minamino, Hiroyuki Kurihara, Katsushige Yamada
  • Publication number: 20150140615
    Abstract: The present invention provides a fed-batch culture method comprising a step of fed-batch-feeding a carbon source base and a base in such a manner that the pH level can be maintained at a level suitable for the growth of microorganisms for fermentation of a carbon source. The present invention also provides a method for preparing organic acids using the fed-batch culture method. The present invention fed-batch-feeds a neutralizing agent such as ammonium bicarbonate, ammonium carbonate or alkali metal-containing weak base, and a carbon source substrate in preparing organic acids by microorganism fermentation. Thus, a pH level suitable for the survival of microorganisms for carbon source fermentation can be maintained, and the speed of injecting the carbon source base which is the source material can be appropriately adjusted.
    Type: Application
    Filed: May 21, 2013
    Publication date: May 21, 2015
    Inventors: Jae Yeon Park, Sin Young Kang, Woo Chan Park, Min Su Koo, In Ho Cho, Joong Min Park, Seung Yeop Lee, Dong Hyun Kim
  • Publication number: 20150140624
    Abstract: A method and system for producing lactic acid from biomass materials uses a mixed bacteria culture of at least one homofermentative lactic acid bacteria and at least one heterofermentative lactic acid bacteria in an integrated production system to increase the productivity and yield of lactic acid.
    Type: Application
    Filed: June 15, 2012
    Publication date: May 21, 2015
    Applicant: Jiangsu University
    Inventor: Fengjie Cui
  • Publication number: 20150140625
    Abstract: A genetically engineered yeast cell capable of producing lactate having increased TPI activity, a method of preparing the yeast cell, and a method of producing lactate by using the yeast cell.
    Type: Application
    Filed: November 13, 2014
    Publication date: May 21, 2015
    Inventors: Juyoung Lee, Changduk Kang, Soyoung Lee, Youngkyoung Park, Jiyoon Song, Seunghyun Lee, Kwangmyung Cho
  • Patent number: 9034608
    Abstract: The invention relates to a polypeptide having a mutation at one or more position corresponding to T219 of SEQ ID NO: 55, wherein the polypeptide has at least 50% sequence identity with SEQ ID NO: 55, and wherein the polypeptide has permease activity.
    Type: Grant
    Filed: October 11, 2011
    Date of Patent: May 19, 2015
    Assignee: DSM IP ASSETS, B.V.
    Inventors: Hendrik Wouter Wisselink, Antonius Jeroen Adriaan Van Maris, Jacobus Thomas Pronk, Paul Klaassen, Rene Marcel De Jong
  • Publication number: 20150132814
    Abstract: Sugar mixtures containing nonfermentable oligomers are fermented in the presence of certain enzymes that depolymerise the oligomers simultaneously with the fermentation process.
    Type: Application
    Filed: October 27, 2014
    Publication date: May 14, 2015
    Applicant: Cargill, Incorporated
    Inventors: Daniel R. Beacom, Jeffrey J. Kolstad, David H. Reeder, Brian J. Rush
  • Publication number: 20150125903
    Abstract: The present invention provides fungal xylanase and/or xylosidase enzymes suitable for use in saccharification reactions. The present invention provides xylanase and xylosidase enzymes suitable for use in saccharification reactions. The present application further provides genetically modified fungal organisms that produce xylanase(s) and/or xylosidase(s), as well as enzyme mixtures exhibiting enhanced hydrolysis of cellulosic material to fermentable sugars, enzyme mixtures produced by the genetically modified fungal organisms, and methods for producing fermentable sugars from cellulose using such enzyme mixtures. In some embodiments, the xylanase and xylosidase enzyme(s) are M. thermophila enzymes.
    Type: Application
    Filed: January 14, 2015
    Publication date: May 7, 2015
    Inventors: Nicholas John Agard, David Elgart, Jie Yang, Goutami Banerjee, Jeanne Bonomo Benoit, Dipnath Baidyaroy
  • Publication number: 20150125902
    Abstract: Provided are isolated polypeptides having glucoamylase activity, catalytic domains, and polynucleotides encoding the polypeptides, catalytic domains. Also provided are nucleic acid constructs, vectors and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides, catalytic domains.
    Type: Application
    Filed: August 24, 2012
    Publication date: May 7, 2015
    Applicant: Novozymes A/S
    Inventors: Tianqi Sun, Ming Li
  • Publication number: 20150125908
    Abstract: A method of producing a sugar liquid from cellulose-containing biomass includes (1) to (3): (1): subjecting a cellulose-containing biomass to hydrothermal treatment and thereafter separating the treated cellulose-containing biomass into a hydrothermally-treated liquid and a cellulose-containing solid content; (2): adding a filamentous fungus-derived cellulase to the cellulose-containing solid content obtained in (1) to hydrolyze the cellulose and thereafter separating the hydrolysate into a saccharification residue and a sugar liquid; and (3): washing the saccharification residue obtained in (2) with the hydrothermally-treated liquid obtained in (1) to elute the filamentous fungus-derived cellulase adsorbed to the saccharification residue into the hydrothermally-treated liquid and thereafter obtaining a solution component comprising the filamentous fungus-derived cellulase by solid-liquid separation.
    Type: Application
    Filed: May 17, 2013
    Publication date: May 7, 2015
    Inventors: Hiroyuki Kurihara, Hiroko Ishizuka, Atsushi Minamino, Katsushige Yamada
  • Publication number: 20150125917
    Abstract: A process for producing D(-) lactic acid in high enatiopurity from lignocellulosic material includes providing a hydrolysate of cellulose polymers prepared from the lignocellulosic material, which includes hexose and pentose sugars and contacting the hydrolysate with the bacterium Lactobacillus coryniformis subsp. Torquens, strain 30 (ATCC 25600) in a fermentation reaction.
    Type: Application
    Filed: May 2, 2013
    Publication date: May 7, 2015
    Inventors: Patrick Walsh, Joachim Venus
  • Publication number: 20150125915
    Abstract: It is intended to provide a fungus of the genus Rhizopus having improved ability to produce lactic acid and a method for producing lactic acid using the fungus. The present invention provides a method for improving the lactate dehydrogenase activity of a fungus of the genus Rhizopus, comprising germinating a spore of a fungus of the genus Rhizopus in a culture medium containing a surfactant under specific conditions to obtain a mycelium, and a method for producing lactic acid using the mycelium of the fungus of the genus Rhizopus.
    Type: Application
    Filed: April 4, 2013
    Publication date: May 7, 2015
    Applicant: Kao Corporation
    Inventors: Yuichi Tsuboi, Fumikazu Takahashi, Yumi Nishimura, Kazuhisa Sawada