Hydroxy Carboxylic Acid Patents (Class 435/146)
  • Patent number: 10450593
    Abstract: This disclosure describes enzymes from the type II (a discrete set of enzymes) fatty acid synthesis (“FAS”) pathway that can be used in combination with thiolases to operate a functional reversal of the ?-oxidation cycle. A combination of thiolases with one or more of 3-oxoacyl-[acyl-carrier-protein] reductase (FabG, others), 3-hydroxyacyl-[acp] dehydratase (FabA, FabZ, others), and enoyl-[acyl-carrier-protein] reductase (FabI, FabK, FabL, FabV, others) yields a functional reversal of the ?-oxidation cycle. If only one or two enzymes are used, the remaining enzymes will be traditional beta oxidation enzymes. Once this cycle is coupled with the appropriate priming and termination pathways, the production of carboxylic acids, alcohols, hydrocarbons, amines and their ?-, ?-, and ?-functionalized derivatives from renewable carbon sources can be achieved.
    Type: Grant
    Filed: January 26, 2015
    Date of Patent: October 22, 2019
    Assignee: William Marsh Rice University
    Inventors: Ramon Gonzalez, James M. Clomburg, Jacob E. Vick
  • Patent number: 10167487
    Abstract: This application describes methods, including non-naturally occurring methods, for biosynthesizing 3-hydroxy-3-methylglutaryl-coA and intermediates thereof, as well as non-naturally occurring hosts for producing 3-hydroxy-3-methylglutaryl-coA. This application also describes methods, including non-naturally occurring methods, for biosynthesizing isoprene and intermediates thereof, as well as non-naturally occurring hosts for producing isoprene.
    Type: Grant
    Filed: August 16, 2016
    Date of Patent: January 1, 2019
    Assignee: INVISTA North America S.à.r.l.
    Inventor: Alex Van Eck Conradie
  • Patent number: 10087470
    Abstract: A non-naturally occurring microbial organism includes a microbial organism having a reductive TCA or Wood-Ljungdahl pathway in which at least one exogenous nucleic acid encoding these pathway enzymes is expressed in a sufficient amount to enhance carbon flux through acetyl-CoA. A method for enhancing carbon flux through acetyl-CoA includes culturing theses non-naturally occurring microbial organisms under conditions and for a sufficient period of time to produce a product having acetyl-CoA as a building block. Another non-naturally occurring microbial organism includes at least one exogenous nucleic acid encoding an enzyme expressed in a sufficient amount to enhance the availability of reducing equivalents in the presence of carbon monoxide or hydrogen, thereby increasing the yield of redox-limited products via carbohydrate-based carbon feedstock.
    Type: Grant
    Filed: July 22, 2015
    Date of Patent: October 2, 2018
    Assignee: Genomatica, Inc.
    Inventors: Anthony P. Burgard, Robin E. Osterhout, Jun Sun, Priti Pharkya
  • Patent number: 10036047
    Abstract: Provided herein are methods for producing an ortho-hydroxylated phenylpropanoid. In one embodiment the method includes culturing a microbe that includes HpaBC activity in the presence of a phenylpropanoid substrate. Also provided are genetically engineered microbes engineered to have greater levels of HpaB and/or HpaC than a control microbe.
    Type: Grant
    Filed: June 10, 2014
    Date of Patent: July 31, 2018
    Assignee: UNIVERSITY OF GEORGIA RESEARCH FOUNDATION, INC.
    Inventors: Yajun Yan, Yuheng Lin
  • Patent number: 10017791
    Abstract: A transformant constructed by introducing a gene which encodes an enzyme having chorismate-pyruvate lyase activity into a coryneform bacterium as a host is capable of efficiently producing 4-hydroxybenzoic acid or a salt thereof from a sugar. When the transformant is cultured under aerobic conditions where the transformant does not grow, 4-hydroxybenzoic acid or a salt thereof can be produced in a particularly efficient manner.
    Type: Grant
    Filed: April 7, 2015
    Date of Patent: July 10, 2018
    Assignee: GREEN CHEMICALS CO., LTD.
    Inventors: Hideaki Yukawa, Masayuki Inui, Kazumi Hiraga, Masako Suda, Ryoma Hashimoto
  • Patent number: 10004769
    Abstract: The present invention relates to a novel Lactobacillus brevis G-101 strain capable of decomposing monosodium L-glutamate (MSG), and a functional health food, a pharmaceutical composition, or a food product comprising the same as an active ingredient. More specifically, the strain is effective in reducing in vivo blood MSG levels of animals and attenuating MSG Symptom Complex, and thus can be used in a functional health food, a pharmaceutical composition, or a food product aiming to prevent in vivo absorption of MSG, which is known to be harmful, and improve the MSG Symptom Complex.
    Type: Grant
    Filed: June 2, 2014
    Date of Patent: June 26, 2018
    Assignee: CTC BIO, INC.
    Inventors: Hong Ryeol Jeon, Yoon-Mo Kang, Byeong-Gon Lee, Se-Young Kim, Go-Eun Shin
  • Patent number: 9926568
    Abstract: The invention provides compositions and methods for clostridial bacteria that have been engineered to produce and/or to improve efficiency of production of industrial bioproducts.
    Type: Grant
    Filed: June 20, 2014
    Date of Patent: March 27, 2018
    Assignee: Danisco US Inc.
    Inventors: Miles C. Scotcher, Derek H. Wells
  • Patent number: 9920341
    Abstract: The present invention relates to isolated polypeptides having peroxygenase activity, and polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides.
    Type: Grant
    Filed: March 9, 2017
    Date of Patent: March 20, 2018
    Assignee: Novozymes A/S
    Inventors: Sara Landvik, Lars Henrik Oestergaard, Lisbeth Kalum
  • Patent number: 9908860
    Abstract: The invention relates to enzymatic methods for epoxidation of a non-cyclic aliphatic alkene, or a terpene.
    Type: Grant
    Filed: April 26, 2017
    Date of Patent: March 6, 2018
    Assignee: Novozymes A/S
    Inventors: Henrik Lund, Lisbeth Kalum, Martin Hofrichter, Sebastian Peter
  • Patent number: 9890400
    Abstract: A method for the preparation of 2,4-dihydroxybutyric acid (2,4-DHB) including the successive steps of converting malate, succinyl-CoA and/or glyoxylate into malyl-CoA, converting malyl-CoA previously obtained into malate-4-semialdehyde, and converting malate-4-semialdehyde into 2,4-DHB using a DHB dehydrogenase.
    Type: Grant
    Filed: April 25, 2013
    Date of Patent: February 13, 2018
    Assignee: ADISSEO FRANCE S.A.S.
    Inventors: Thomas Walther, Clèmentine Dressaire, Hélène Cordier, Jean-Marie Francois
  • Patent number: 9856500
    Abstract: The present invention pertains to a method for consolidated bio processing of lignocellulosic biomass to L-Lactic acid. Particularly, the present invention relates to the production of L-Lactic Acid from low cost non edible feedstock lignocellulosic biomass. More particularly the present invention relates to the process for one step production of L-Lactic Acid from lignocellulosic biomass using thermophilic bacteria Paenibacillus macerans IIPSP3 (MTCC 5569), which is not only capable of hydrolyzing cellulose to glucose but also further fermenting it to L-Lactic Acid under aerobic conditions, without any growth inhibition in presence of lignin. The present invention provides a process which has less chances of contamination, as the fermentation is carried out at higher temperatures and is economically attractive, as preferably no external enzyme loadings are required.
    Type: Grant
    Filed: July 17, 2013
    Date of Patent: January 2, 2018
    Assignee: Council of Scientific and Industrial Research
    Inventors: Dilip Kumar Adhikari, Jayati Trivedi, Deepti Agrawal
  • Patent number: 9845484
    Abstract: Provided herein are recombinant yeast cells having an active 3-Hydroxypropionic Acid (3-HP) pathway and further comprising a heterologous polynucleotide encoding an aspartate 1-decarboxylase (ADC) of the Class Insecta, Bivalvia, Branchioporia, Gastropoda, or Leptocardii. Also described are methods of using the recombinant yeast cells to produce 3-HP and acrylic acid.
    Type: Grant
    Filed: July 31, 2014
    Date of Patent: December 19, 2017
    Assignee: NOVOZYMES A/S
    Inventors: Michelle Barnhart, Ana Negrete-Raymond, Janice Frias, Gui Barbier, Michael Catlett
  • Patent number: 9834796
    Abstract: The present invention relates to a method for producing useful metabolites such as shikimic acid from filamentous fungus. The useful metabolites can be produced by a production method involving a step of inhibiting the growth of the filamentous fungus, specifically by applying a stimulus of light having a center wavelength shorter than 570 nm to the filamentous fungus, to increase the content of the useful metabolite in a hypha of the filamentous fungus.
    Type: Grant
    Filed: August 6, 2012
    Date of Patent: December 5, 2017
    Assignee: Shinshu University
    Inventors: Masanobu Kojima, Hiroshi Fujii
  • Patent number: 9777294
    Abstract: The invention features methods for producing isoprene from cultured cells wherein the cells in the stationary phase. The invention also provides compositions that include these cultured cells and/or increased amount of isoprene. The invention also provides for systems that include a non-flammable concentration of isoprene in the gas phase. Additionally, the invention provides isoprene compositions, such as compositions with increased amount of isoprene or increased purity.
    Type: Grant
    Filed: December 17, 2015
    Date of Patent: October 3, 2017
    Assignees: Danisco US Inc., The Goodyear Tire & Rubber Company
    Inventors: Anthony R. Calabria, Marguerite A. Cervin, Gopal K. Chotani, Richard La Duca, Joseph C. McAuliffe, Michael C. Miller, Timothy A. Sabo, Karl J. Sanford, Erin L. Spring, Gregory M. Whited
  • Patent number: 9580738
    Abstract: The disclosure provides a method for producing extracellular proteins from genus Tepidimonas, including: performing a fermentation culturing to a bacteria of genus Tepidimonas with a culturing medium to form a fermented liquid, wherein the composition of the culturing medium includes a carbon source which is an organic acid, selected from a group consisting of acetate, lactate and butyrate; a nitrogen source selected from a group consisting of (NH4)2SO4, NH4NO3, NH4Cl and urea; phosphate; carbonate; MgCl2; yeast extract; and trace elements, and wherein the gas feeding rate for the fermentation culturing is about 0-0.1 vvm; and after the fermentation culturing is completed, collecting the fermented liquid, wherein the fermented liquid contains extracellular protein secreted from the bacteria of genus Tepidimonas.
    Type: Grant
    Filed: August 28, 2015
    Date of Patent: February 28, 2017
    Assignee: INDUSTRIAL TECHNOLOGY RESEARCH INSTITUTE
    Inventors: Yin-Lung Han, Jo-Shu Chang, Yung-Chong Lou, Chieh-Lun Cheng, Chih-Hsi Liu
  • Patent number: 9556460
    Abstract: Described is a method for the production of 3-hydroxy-3-methylbutyric acid by enzyme-catalyzed covalent bond formation between the carbon atom of the oxo group of acetone and the methyl group of a compound which provides an activated acetyl group. Also described are recombinant organisms which produce 3-hydroxy-3-methylbutyric acid, and related compositions and methods.
    Type: Grant
    Filed: March 14, 2015
    Date of Patent: January 31, 2017
    Assignee: Scientist of Fortune, S.A.
    Inventor: Philippe Marliere
  • Patent number: 9540652
    Abstract: The present disclosure relates to engineered microorganisms that produce amino acids and amino acid intermediates. In particular, the disclosure relates to recombinant nucleic acids encoding operons that increase production of aromatic amino acids and the aromatic amino acid intermediate shikimate; microorganisms with increased production of aromatic amino acids and the aromatic amino acid intermediate shikimate; and methods related to the production of aromatic amino acids, the aromatic amino acid intermediate shikimate, and commodity chemicals derived therefrom.
    Type: Grant
    Filed: August 31, 2012
    Date of Patent: January 10, 2017
    Assignee: The Regents of the University of California
    Inventors: Darmawi Juminaga, Jay D. Keasling
  • Patent number: 9534238
    Abstract: The invention relates to enzymatic methods for hydroxylation in position 2 or 3 of substituted or unsubstituted, linear or branched aliphatic hydrocarbons.
    Type: Grant
    Filed: September 2, 2015
    Date of Patent: January 3, 2017
    Assignee: NOVOZYMES A/S
    Inventors: Martin Hofrichter, Katrin Scheibner, Rene Ullrich, Matthias Kinne, Sebastian Peter, Henrik Lund, Lisbeth Kalum
  • Patent number: 9404115
    Abstract: Provided is a phenol-producing transformant constructed by transferring a gene which encodes an enzyme having chorismate-pyruvate lyase activity and a gene which encodes an enzyme having 4-hydroxybenzoate decarboxylase activity into a coryneform bacterium as a host. Also provided is a process for producing phenol, which comprises a step of allowing the transformant to react in a reaction mixture containing a saccharide under reducing conditions, and a step of collecting phenol from the reaction mixture.
    Type: Grant
    Filed: November 9, 2011
    Date of Patent: August 2, 2016
    Assignee: GREEN PHENOL DEVELOPMENT CO., LTD.
    Inventors: Hideaki Yukawa, Masayuki Inui
  • Patent number: 9353375
    Abstract: Provided herein are improved compositions and methods for the increased production of isoprene. Also provided herein are improved compositions and methods for the increased production of heterologous polypeptides capable of biological activity.
    Type: Grant
    Filed: October 7, 2014
    Date of Patent: May 31, 2016
    Assignees: DANISCO US INC., THE GOODYEAR TIRE & RUBBER COMPANY
    Inventors: Zachary Q. Beck, Marguerite A. Cervin, Alex T. Nielsen, Caroline M. Peres
  • Patent number: 9200296
    Abstract: Methods for producing an isoprenoid are provided. A plurality of bacterial or fungal host cells is obtained. These cells comprise a heterologous nucleic acid encoding one or more enzymes of a mevalonate pathway for making isopentenyl pyrophosphate. Expression of the one or more enzymes is under control of at least one heterologous transcriptional regulator. The mevalonate pathway comprises (i) an enzyme that condenses acetoacetyl-CoA with acetyl-CoA to form HMG-CoA, (ii) an enzyme that converts HMG-CoA to mevalonate, (iii) an enzyme that phosphorylates mevalonate to mevalonate 5-phosphate, (iv) an enzyme that converts mevalonate 5-phosphate to mevalonate 5-pyrophosphate, and (v) an enzyme that converts mevalonate 5-pyrophosphate to isopentenyl pyrophosphate. The host cells are cultured in a medium under conditions that are suboptimal as compared to conditions for the maximum growth rate. Temperature is maintained at a level below that which would provide for a maximum specific growth rate for the host cells.
    Type: Grant
    Filed: March 20, 2013
    Date of Patent: December 1, 2015
    Assignee: Amyris Inc.
    Inventors: Neil Stephen Renninger, Jack Newman, Keith Kinkead Reiling, Rika Regentin, Christopher John Paddon
  • Patent number: 9133444
    Abstract: Hydroxycarboxylic acids are produced by using a microorganism that is improved in ability to produce nicotinamide adenine dinucleotide by deleting, mutating or substituting nadR gene in the microorganism or introducing a gene encoding nicotinic acid phosphoribosyltransferase.
    Type: Grant
    Filed: September 14, 2012
    Date of Patent: September 15, 2015
    Assignee: MITSUI CHEMICALS, INC.
    Inventors: Takashi Morishige, Mitsufumi Wada, Hitoshi Takahashi, Daisuke Mochizuki, Junko Tokuda
  • Patent number: 9109236
    Abstract: A non-naturally occurring microbial organism having an isopropanol, 4-hydroxybutryate, or 1,4-butanediol pathway includes at least one exogenous nucleic acid encoding an isopropanol, 4-hydroxybutryate, or 1,4-butanediol pathway enzyme expressed in a sufficient amount to produce isopropanol, 4-hydroxybutryate, or 1,4-butanediol. The aforementioned organisms are cultured to produce isopropanol, 4-hydroxybutryate, or 1,4-butanediol.
    Type: Grant
    Filed: June 18, 2013
    Date of Patent: August 18, 2015
    Assignee: GENOMATICA, INC.
    Inventors: John D. Trawick, Mark J. Burk, Anthony P. Burgard
  • Patent number: 9090900
    Abstract: Provided is a phenol-producing transformant constructed by transferring a gene which encodes an enzyme having 4-hydroxybenzoate decarboxylase activity into Corynebacterium glutamicum as a host. Also provided is a process for producing phenol, which comprises a step of allowing the transformant to react in a reaction mixture containing 4-hydroxybenzoate or a salt thereof under reducing conditions, and a step of collecting phenol from the reaction mixture.
    Type: Grant
    Filed: November 9, 2011
    Date of Patent: July 28, 2015
    Assignee: GREEN PHENOL DEVELOPMENT CO., LTD.
    Inventors: Hideaki Yukawa, Masayuki Inui
  • Patent number: 9085784
    Abstract: Embodiments of the invention relate generally to methods to generate microorganisms and/or microorganism cultures that exhibit the ability to produce polyhydroxyalkanoates (PHA) from carbon sources at high efficiencies. In several embodiments, preferential expression of, or preferential growth of microorganisms utilizing certain metabolic pathways, enables the high efficiency PHA production from carbon-containing gases or materials. Several embodiments relate to the microorganism cultures, and/or microorganisms isolated therefrom.
    Type: Grant
    Filed: March 13, 2013
    Date of Patent: July 21, 2015
    Assignee: Newlight Technologies, LLC
    Inventor: Markus D. Herrema
  • Publication number: 20150147793
    Abstract: A method for the preparation of 2,4-dihydroxybutyric acid from homoserine includes a first step of conversion of the primary amino group of homoserine to a carbonyl group to obtain 2-oxo-4-hydroxybutyrate, and a second step of reduction of the obtained 2-oxo-4-hydroxybutyrate (OHB) to 2,4-dihydroxybutyrate.
    Type: Application
    Filed: July 10, 2013
    Publication date: May 28, 2015
    Inventors: Thomas Walther, Hélène Cordier, Clémentine Dressaire, Jean Marie Francois, Robert Huet
  • Publication number: 20150140615
    Abstract: The present invention provides a fed-batch culture method comprising a step of fed-batch-feeding a carbon source base and a base in such a manner that the pH level can be maintained at a level suitable for the growth of microorganisms for fermentation of a carbon source. The present invention also provides a method for preparing organic acids using the fed-batch culture method. The present invention fed-batch-feeds a neutralizing agent such as ammonium bicarbonate, ammonium carbonate or alkali metal-containing weak base, and a carbon source substrate in preparing organic acids by microorganism fermentation. Thus, a pH level suitable for the survival of microorganisms for carbon source fermentation can be maintained, and the speed of injecting the carbon source base which is the source material can be appropriately adjusted.
    Type: Application
    Filed: May 21, 2013
    Publication date: May 21, 2015
    Inventors: Jae Yeon Park, Sin Young Kang, Woo Chan Park, Min Su Koo, In Ho Cho, Joong Min Park, Seung Yeop Lee, Dong Hyun Kim
  • Patent number: 9034615
    Abstract: The present invention provides a method for the biological production of glycolic acid from a fermentable carbon source in a microorganism. In one aspect of the present invention, a process for the conversion of glucose to glycolic acid is achieved by the use of a recombinant organism comprising a host E. coli transformed i) to attenuate the glyoxylate consuming pathways to other compounds than glycolate ii) to use an NADPH glyoxylate reductase to convert glyoxylate to glycolate iii) to attenuate the level of all the glycolate metabolizing enzymes and iv) increase the flux in the glyoxylate pathway. In another aspect of the present invention, the process for the production of glycolic acid from a fermentable carbon source, using a recombinant E. coli, is improved by increasing the NADPH availability in the cells.
    Type: Grant
    Filed: June 7, 2007
    Date of Patent: May 19, 2015
    Assignee: Metabolic Explorer
    Inventor: Philippe Soucaille
  • Publication number: 20150125919
    Abstract: A recombinant microorganism having increased iron-regulated ABC transporter activity and increased hydroxycarboxylic acid production, as well as a method of producing a hydroxycarboxylic acid using the recombinant microorganism, and a method of producing the recombinant microorganism.
    Type: Application
    Filed: November 5, 2014
    Publication date: May 7, 2015
    Inventors: Hwayoung Cho, Jinhwan Park, Hongsoon Rhee, Kwangmyung Cho
  • Publication number: 20150126598
    Abstract: The present invention relates to a concentrated extract of cranberry (Vaccinium macrocarpon), the complex composition of which makes it possible to increase the antibacterial effects thereof, which is of use for the prevention or treatment of urinary infections, and in particular for the preventive treatment of urinary infections or treatment against the recurrence thereof. The invention also relates to a process for preparing such an extract, to food, nutraceutical or pharmaceutical compositions comprising the extract and to the use thereof in the treatment or prevention of urinary infections.
    Type: Application
    Filed: June 7, 2013
    Publication date: May 7, 2015
    Inventors: Philippe Sanoner, Valerie Bochard, Lucie Charissou, Benedicte Lastique, Morgane Jacob, Patrice Thomas
  • Publication number: 20150125906
    Abstract: The present invention relates to methods of degrading or converting biomass material enriched with hemicellulosic material into fermentable sugars.
    Type: Application
    Filed: January 14, 2015
    Publication date: May 7, 2015
    Inventors: Prashant Iyer, Harry Showmaker, Hui Xu, Kishore Rane
  • Publication number: 20150128301
    Abstract: Nucleic acid molecules from cannabis has been isolated and characterized and encode polypeptides having aromatic prenyltransferase activity. Expression or over-expression of the nucleic acids alters levels of cannabinoid compounds. The polypeptides may be used in vivo or in vitro to produce cannabinoid compounds.
    Type: Application
    Filed: October 9, 2014
    Publication date: May 7, 2015
    Inventors: Jonathan E. Page, Zakia Boubakir
  • Publication number: 20150125912
    Abstract: The invention provides a process for the preparation of hydrocarbons substituted with at least one group containing at least one oxygen atom, comprising the process steps A) reaction of a carbon source comprising at least one selected from CO2 and CO to give acetate and/or ethanol with a first microorganism, B) separating off of the acetate from the first microorganism, C) reaction of the acetate to give a hydrocarbon substituted with at least one group containing at least one oxygen atom with a second microorganism and optionally D) purification of the hydrocarbon substituted with at least one group containing at least one oxygen atom.
    Type: Application
    Filed: May 8, 2013
    Publication date: May 7, 2015
    Applicant: Evonik Industries AG
    Inventors: Thomas Haas, Eva Maria Wittmann
  • Publication number: 20150125918
    Abstract: A microorganism with improved acid-resistance. A microorganism capable of efficiently producing 3-HP, and methods for producing an organic acid.
    Type: Application
    Filed: November 3, 2014
    Publication date: May 7, 2015
    Inventors: Juyoung Lee, Hyunmin Koo, Jaechan Park, Jieun Kim, Jinha Kim, Joonsong Park, Soonchun Chung, Byungkwan Cho
  • Patent number: 9017977
    Abstract: Described is a method for the production of 3-hydroxy-3-methylbutyric acid by enzyme-catalyzed covalent bond formation between the carbon atom of the oxo group of acetone and the methyl group of a compound which provides an activated acetyl group. Also described are recombinant organisms which produce 3-hydroxy-3-methylbutyric acid, and related compositions and methods.
    Type: Grant
    Filed: September 14, 2010
    Date of Patent: April 28, 2015
    Assignee: Scientist of Fortune S.A.
    Inventor: Philippe Marlière
  • Publication number: 20150111262
    Abstract: This document describes biochemical pathways for producing glutaric acid, 5-aminopentanoic acid, 5-hydroxypentanoic acid, cadaverine or 1,5-pentanediol by forming one or two terminal functional groups, comprised of carboxyl, amine or hydroxyl group, in a C5 backbone substrate such as D-proline.
    Type: Application
    Filed: August 27, 2014
    Publication date: April 23, 2015
    Inventors: Adriana Leonora Botes, Alex Van Eck Conradie
  • Publication number: 20150093795
    Abstract: The present invention relates tocutinasevariants. The present invention also relates to polynucleotides encoding the variants; nucleic acid constructs, vectors, and host cells comprising the polynucleotides; and methods of using the variants.
    Type: Application
    Filed: April 30, 2013
    Publication date: April 2, 2015
    Inventors: Aditya Basu, Naik Sangeeta, Santhosh Mepadam Vasu, Paul Pritish, Rakhi Saikia, Allan Svendsen
  • Publication number: 20150087036
    Abstract: Provided herein is an alkane-metabolizing cell that is unable to convert propionyl-CoA into methylmalonyl-CoA or 2-metylcitrate synthase. Depending on which enzymes are present in the cell, the cell can produce acrylate or a precursor for the same (e.g., propionate, 3-hydroxypropionyl-CoA, 3-hydroxypropionate, acrylyl-CoA) that can be readily converted to acrylate enzymatically (e.g., in the cell) or by chemical treatment. In one embodiment, the cell may contain a cytochrome P450 or alkane oxidase enzyme that allows the production of 3-hydroxypropionyl-CoA, which can be readily converted to 3-hydroxypropionate. In order to make such compounds, the cell may be grown in the presence of an odd-numbered chain alkane (e.g., pentane or heptane), although another odd-numbered chain alkane may be used. In another embodiment, the cell may contain acyl-CoA oxidase, enoyl-CoA hydratase, and hydrolase.
    Type: Application
    Filed: October 17, 2012
    Publication date: March 26, 2015
    Inventors: Joshua A. Silverman, Tom Purcell, Jon Edward Ness, Effendi Leonard
  • Publication number: 20150087031
    Abstract: The present invention relates to methods of processing lignocellulosic material to obtain hemicellulose sugars, cellulose sugars, lignin, cellulose and other high-value products. Also provided are hemicellulose sugars, cellulose sugars, lignin, cellulose, and other high-value products.
    Type: Application
    Filed: May 3, 2013
    Publication date: March 26, 2015
    Inventors: Robert Jansen, Claire Gregoire, philip Travisano, Lee Madsen, Neta Matis, Yael Har-Tal, Shay Eliahu, James Alan Lawson, Noa Lapidot, Luke Burke, Aharon M. Eyal, Timothy Allen Bauer, Hagit Sade, Paul Mcwilliams, Ziv-Vladimir Belman, Bassem Hallac, Michael Zviely, Yelena Gershinksy, Adam Carden
  • Publication number: 20150082493
    Abstract: The present invention relates to GH61 polypeptide variants. The present invention also relates to polynucleotides encoding the variants; nucleic acid constructs, vectors, and host cells comprising the polynucleotides; and methods of using the variants.
    Type: Application
    Filed: April 26, 2013
    Publication date: March 19, 2015
    Inventors: Janine Lin, Doreen Bohan, Michelle Maranta, Leslie Beresford, Michael Lamsa, Matt Sweeney, Mark Wogulis, Elizabeth Znameroski, Frank Winther Rasmussen
  • Patent number: 8980594
    Abstract: The invention relates to the use of a protein homologous to a MeaB protein for increasing the enzymatic activity of a 3-hydroxycarboxylic acid-CoA mutase, a fusion protein comprising a 3-hydroxycarboxylic acid-CoA mutase and a protein sequence homologous to a MeaB protein and an enzymatic method for producing 2-hydroxyisobutryric acid.
    Type: Grant
    Filed: October 11, 2010
    Date of Patent: March 17, 2015
    Assignee: Evonik Roehm GmbH
    Inventors: Liv Reinecke, Steffen Schaffer, Tim Koehler, Anja Thiessenhusen, Achim Marx, Markus Buchhaupt
  • Patent number: 8980578
    Abstract: The present invention provides fungal xylanase and/or beta-xylosidase enzymes suitable for use in saccharification reactions. The present application further provides genetically modified fungal organisms that produce xylanase and/or beta-xylosidases, as well as enzyme mixtures exhibiting enhanced hydrolysis of cellulosic material to fermentable sugars, enzyme mixtures produced by the genetically modified fungal organisms, and methods for producing fermentable sugars from cellulose using such enzyme mixtures.
    Type: Grant
    Filed: June 10, 2013
    Date of Patent: March 17, 2015
    Assignee: Codexis, Inc.
    Inventors: Ryan Fong, Xiyun Zhang, Chris Noriega, Nicholas Agard, Anupam Gohel, Derek Smith
  • Publication number: 20150072386
    Abstract: To provide a method for producing an organic acid, whereby the desired organic acid can be efficiently recovered without necessity for adjusting the pH to a neutral level in the fermentation step. The method for producing an organic acid, comprises a first step of producing an organic acid by fermentation to obtain a crude liquid containing the organic acid and having a pH of from 1 to 5, and a second step of extracting the organic acid from the crude liquid containing the organic acid obtained in the first step by means of an extraction medium containing a C10-30 diester compound and an alkylamine compound to obtain an extract (1).
    Type: Application
    Filed: November 12, 2014
    Publication date: March 12, 2015
    Applicant: ASAHI GLASS COMPANY, LIMITED
    Inventors: Tatsuhiro . NOGAMI, Nobuyuki KASAHARA, Tsubasa TAKASUGI
  • Publication number: 20150064755
    Abstract: Recombinant bacteria capable of metabolizing sucrose are described. The recombinant bacteria comprise in their genome or on at least one recombinant construct, a novel nucleotide sequence encoding a polypeptide having sucrose transporter activity and a nucleotide sequence encoding a polypeptide having sucrose hydrolase activity. These nucleotide sequences are each operably linked to the same or a different promoter. Recombinant bacteria capable of metabolizing sucrose to produce glycerol and/or glycerol-derived products such as 1,3-propanediol and 3-hydroxypropionic acid are also described.
    Type: Application
    Filed: March 5, 2013
    Publication date: March 5, 2015
    Applicant: E I DU PONT DE NEMOURS AND COMPANY
    Inventors: Kristin Ruebling-Jass, Jean-Francois Tomb, Tina K Van Dyk, Zheng You
  • Publication number: 20150064754
    Abstract: The present invention provides various combinations of genetic modifications to a transformed host cell that provide increase conversion of carbon to a chemical product. The present invention also provides methods of fermentation and methods of making various chemical products.
    Type: Application
    Filed: March 17, 2014
    Publication date: March 5, 2015
    Inventors: Hans LIAO, Christopher Patrick MERCOGLIANO, Travis Robert WOLTER, Michael Tai Man LOUIE, Wendy Kathleen RIBBLE, Tanya LIPSCOMB, Eileen Colie SPINDLER, Michael D LYNCH
  • Publication number: 20150064751
    Abstract: The embodiments described herein pertain to cells, and methods for preparing cells, that can be used as biocatalysts by altering enzymes that compete for a substrate or product of a pathway of interest such that the targeted enzyme is sensitive to a site-specific protease, which protease is expressed but relocated in the cell to a site where it is not in contact with the targeted enzyme in the intact cell. Upon cell lysis, the protease contacts the target enzyme, which is then inactivated by protease cleavage.
    Type: Application
    Filed: November 14, 2014
    Publication date: March 5, 2015
    Applicants: The Board of Trustees of the Leland Stanford Junior University, GreenLight Biosciences, Inc.
    Inventor: James R. Swartz
  • Patent number: 8969048
    Abstract: The present invention provides a bacterium which has an ability to produce a useful metabolite derived from acetyl-coenzyme A, such as L-glutamic acid, L-glutamine, L-proline, L-arginine, L-leucine, L-cysteine, succinate, and polyhydroxybutyrate, wherein said bacterium is modified so that activities of D-xylulose-5-phosphate phosphoketolase and/or fructose-6-phosphate phosphoketolase are enhanced. The present invention also provides a method for producing the useful metabolite using the bacterium.
    Type: Grant
    Filed: May 2, 2014
    Date of Patent: March 3, 2015
    Assignee: Ajinomoto Co., Inc.
    Inventors: Yury Ivanovich Kozlov, Akito Chinen, Hiroshi Izui, Yoshihiko Hara, Hisashi Yasueda, Konstantin Vyacheslavovich Rybak, Ekaterina Aleksandrovna Slivinskaya, Joanna Yosifovna Katashkina
  • Publication number: 20150057465
    Abstract: The present invention provides various combinations of genetic modifications to a transformed host cell that provide increase conversion of carbon to a chemical product. The present invention also provides methods of fermentation and methods of making various chemical products.
    Type: Application
    Filed: March 17, 2014
    Publication date: February 26, 2015
    Inventors: Hans LIAO, Christopher Patrick MERCOGLIANO, Travis Robert WOLTER, Michael Tai Man LOUIE, Wendy Kathleen RIBBLE, Tanya LIPSCOMB, Eileen Colie SPINDLER, Michael D. LYNCH
  • Publication number: 20150056669
    Abstract: The present invention provides various combinations of genetic modifications to a transformed host cell that provide increase conversion of carbon to a chemical product. The present invention also provides methods of fermentation and methods of making various chemical products.
    Type: Application
    Filed: March 17, 2014
    Publication date: February 26, 2015
    Inventors: Hans LIAO, Christopher Patrick MERCOGLIANO, Travis Robert WOLTER, Michael Tai Man LOUIE, Wendy Kathleen RIBBLE, Tanya LIPSCOMB, Eileen Colie SPINDLER, Michael D LYNCH
  • Publication number: 20150056651
    Abstract: This invention relates to metabolically engineered microorganism strains, such as bacterial strains, in which there is an increased utilization of malonyl-CoA for production of a chemical product, which includes polyketides and 3-hydroxypropionic acid.
    Type: Application
    Filed: April 7, 2014
    Publication date: February 26, 2015
    Inventors: Michael D. Lynch, Ryan T. Gill, Tanya E.W. Lipscomb