Hydroxy Carboxylic Acid Patents (Class 435/146)
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Patent number: 7867736Abstract: The invention relates to a method for preparing a complex of an organic amine and lactic acid in a reactor, characterized in that a water-miscible organic amine is brought into contact with magnesium lactate in an aqueous medium in the reactor to form the complex and precipitated magnesium hydroxide, after which the magnesium hydroxide is separated from the complex.Type: GrantFiled: March 2, 2007Date of Patent: January 11, 2011Assignee: Purac Biochem B.V.Inventor: Jan Van Krieken
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Publication number: 20100330630Abstract: The invention provides spray-dried preparations of microbes and methods of using those microbes.Type: ApplicationFiled: November 16, 2007Publication date: December 30, 2010Applicant: University of Iowa Research FoundationInventors: Venkiteswaran Subramanian, James H. Glenn IV, Shuvendu Das
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Publication number: 20100323390Abstract: The invention relates to the direct selection of metabolic pathways having a determined function in the transformation of a substrate {Ai} into a target product {B}, which is of interest in the industrial, pharmaceutical or agri-food sectors. More specifically, the invention relates to the detection, within metagenomic libraries, of novel biosynthesis pathways involved in a biochemical reaction having a known product {B}. The selection and characterisation of said novel metabolic pathways enables {B} to be produced enzymatically. The invention provides an alternative to the chemical synthesis of the molecule in question {B}. Moreover, and above all, the invention can be used specifically to target and exploit the only metabolic pathways enabling the transformation of {Ai} into {B}, while eliminating the associated metabolic pathways that can catabolise the target product {B}.Type: ApplicationFiled: August 31, 2010Publication date: December 23, 2010Applicant: LIBRAGENInventors: Pierre Monsan, Claude Bensoussan, Philippe Reulet, Renaud Nalin, Patrick Robe, Karine Tuphile, Benjamin Gillet, Pierre Pujic
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Publication number: 20100319091Abstract: A method of enhancing production of a phenylpropanoid compound in a plant or plant cell is disclosed. The method comprising: (a) expressing in the plant or plant cell a heterologous polynucleotide which produces a myb gene of the phenylpropanoid pathway; and (b) contacting the plant or plant cell with at least one substrate of the phenylpropanoid pathway, the substrate being upstream to the production of the phenylpropanoid compound, thereby enhancing the production of a phenylpropanoid compound in the plant or plant cell.Type: ApplicationFiled: November 13, 2008Publication date: December 16, 2010Applicant: YISSUM RESEARCH DEVELOPMENT COMPANY OF THE HEBREWInventors: Alexander Vainstein, Michal Moyal-Ben-Zvi, Ben Rimon-Spitzer
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Publication number: 20100317069Abstract: The invention provides a non-naturally occurring microbial organism having a 6-aminocaproic acid, caprolactam, hexametheylenediamine or levulinic acid pathway. The microbial organism contains at least one exogenous nucleic acid encoding an enzyme in the respective 6-aminocaproic acid, caprolactam, hexametheylenediamine or levulinic acid pathway. The invention additionally provides a method for producing 6-aminocaproic acid, caprolactam, hexametheylenediamine or levulinic acid. The method can include culturing a 6-aminocaproic acid, caprolactam or hexametheylenediamine producing microbial organism, where the microbial organism expresses at least one exogenous nucleic acid encoding a 6-aminocaproic acid, caprolactam, hexametheylenediamine or levulinic acid pathway enzyme in a sufficient amount to produce the respective product, under conditions and for a sufficient period of time to produce 6-aminocaproic acid, caprolactam, hexametheylenediamine or levulinic acid.Type: ApplicationFiled: May 7, 2010Publication date: December 16, 2010Inventors: Mark J. Burk, Anthony P. Burgard, Robin E. Osterhout, Priti Pharkya
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Publication number: 20100311133Abstract: An electrode that can generate reduced coenzymes from a desired substrate in multiple stages is provided. An electrode includes a 2-hydroxycarboxylic acid synthase, a 2-oxo acid synthase, and a 2-oxo acid carboxylyase. The electrode includes a 2-oxo acid carboxylyase, which is a decarboxylase, in addition to a 2-hydroxycarboxylic acid synthase and a 2-oxo acid synthase, which are oxidoreductases; therefore, oxidation products having carboxyl groups, reactions of which do not proceed with oxidoreductases, can be decarboxylated and modified into substances with which oxidoreductases can act. Thus, oxidation reactions and decarboxylation reactions can repeatedly proceed with respect to desired substrates, and large quantities of reduced coenzymes can be generated thereby.Type: ApplicationFiled: December 4, 2008Publication date: December 9, 2010Applicants: SONY CORPORATION, AMANO ENZYME INC.Inventors: Yuichi Tokita, Hideki Sakai, Toshiyuki Sugiura, Chinatsu Goto, Tetsuya Takahashi
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Publication number: 20100304453Abstract: A non-naturally occurring microbial organism having an isopropanol, 4-hydroxybutryate, or 1,4-butanediol pathway includes at least one exogenous nucleic acid encoding an isopropanol, 4-hydroxybutryate, or 1,4-butanediol pathway enzyme expressed in a sufficient amount to produce isopropanol, 4-hydroxybutryate, or 1,4-butanediol. The aforementioned organisms are cultured to produce isopropanol, 4-hydroxybutryate, or 1,4-butanediol.Type: ApplicationFiled: December 16, 2009Publication date: December 2, 2010Applicant: Genomatica, Inc.Inventors: John D. Trawick, Mark J. Burk, Anthony P. Burgard
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Patent number: 7833767Abstract: The present invention is directed to variant polypeptides having enhanced ketoreductase activity and/or thermostability for use in the stereospecific reduction of ketones. In addition, the present invention is directed to polynucleotides that encode the ketoreductase polypeptides, including codon optimized versions of the polynucleotides which provide for enhanced expression in host cells. In another aspect, the present invention is directed to nucleotide constructs, vectors and host cells that are transformed with polynucleotides of the present invention.Type: GrantFiled: October 8, 2009Date of Patent: November 16, 2010Assignee: Codexis, Inc.Inventors: S. Christopher Davis, Gjalt W. Huisman, Stephane J. Jenne, Anke Krebber, Lisa Marie Newman
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Publication number: 20100279368Abstract: A bioengineered synthesis scheme for the production of quinic acid from a carbon source is provided. Methods of producing quinic acid from a carbon source based on the synthesis scheme as well as conversion of quinic acid to hydroquinone are also provided.Type: ApplicationFiled: November 20, 2009Publication date: November 4, 2010Inventors: John W. Frost, Karen M. Frost
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Publication number: 20100273659Abstract: The invention relates to nitrilases and to nucleic acids encoding the nitrilases. In addition methods of designing new nitrilases and method of use thereof are also provided. The nitrilases have increased activity and stability at increased pH and temperature.Type: ApplicationFiled: February 29, 2008Publication date: October 28, 2010Applicant: VERENIUM CORPORATIONInventors: Grace DeSantis, Jennifer Chaplin, Ellen Chi, Aileen Milan, Jay Short, David Weiner, Mark Madden, Darcy Madden, Dan Robertson
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Publication number: 20100240110Abstract: A process is provided to improve the specific activity of an enzyme catalyst having nitrilase activity when converting glycolonitrile to glycolic acid under aqueous reaction conditions. Inclusion of an effective amount of at least one amine protectant improves the specific activity and catalytic productivity of the enzyme catalyst.Type: ApplicationFiled: April 24, 2009Publication date: September 23, 2010Inventors: ROBERT DICOSIMO, Anna Panova, Henry Keith Chenault, Samuel David Arthur
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Publication number: 20100240111Abstract: A process is provided to improve the specific activity of an enzyme catalyst having nitrilase activity when converting glycolonitrile to glycolic acid under aqueous reaction conditions. Inclusion of an effective amount of at least one amine protectant improves the specific activity and catalytic productivity of the enzyme catalyst.Type: ApplicationFiled: April 24, 2009Publication date: September 23, 2010Inventors: Robert DICOSIMO, Anna Panova, Henry Keith Chenault, Samuel David Arthur
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Publication number: 20100240109Abstract: A process is provided to improve the specific activity of an enzyme catalyst having nitrilase activity when converting glycolonitrile to glycolic acid under aqueous reaction conditions. Inclusion of an effective amount of at least one amine protectant improves the specific activity and catalytic productivity of the enzyme catalyst.Type: ApplicationFiled: April 24, 2009Publication date: September 23, 2010Inventors: ROBERT DICOSIMO, Anna Panova, Henry Keith Chenault, Samuel David Arthur
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Publication number: 20100221797Abstract: A process is provided to improve the specific activity of an enzyme catalyst having nitrilase activity when converting glycolonitrile to glycolic acid under aqueous reaction conditions. Inclusion of an effective amount of at least one amine protectant improves the specific activity and catalytic productivity of the enzyme catalyst.Type: ApplicationFiled: April 24, 2009Publication date: September 2, 2010Inventors: Robert Dicosimo, Anna Panova, Henry Keith Chenault, Samuel David Arthur
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Publication number: 20100216199Abstract: The invention is a large-scale production and purification of beta-poly(L-malic acid), biodegradable natural polyester of L-malic acid of molecular weight 30,000 to 300,000 from plasmodia of the Physarum family, especially Physarum polycephalum. This will replace previous laborious and uncontrolled production by bioreactor methods of high productivity and quality, especially to obtain pure polymer of high molecular weight. The described 20-liter bioreactor method can be scaled up for industrial purpose at optimal production and minimum growth time to prevent degradation The invention includes the method of isolation of polymalic acid of 100,000 Mn (number-averaged molecular weight) from the culture broth optimized towards high yields of extremely pure polymalic acid. The non-hygroscopic, water and organic solvent-soluble polymer is endotoxin and agglutinin free and ready to use in chemical syntheses.Type: ApplicationFiled: February 23, 2009Publication date: August 26, 2010Inventor: Eggehard Holler
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Publication number: 20100196976Abstract: A process is provided to improve the specific activity of an enzyme catalyst having nitrilase activity when converting glycolonitrile to glycolic acid under aqueous reaction conditions. Inclusion of an effective amount of at least one amine protectant improves the specific activity and catalytic productivity of the enzyme catalyst.Type: ApplicationFiled: April 24, 2009Publication date: August 5, 2010Inventors: ROBERT DICOSIMO, Anna Panova, Henry Keith Chenault, Samuel David Arthur
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Publication number: 20100196964Abstract: The present invention relates to a process for the production of an aqueous glucose solution from maize or maize kernels. The invention also relates to a glucose solution obtainable by this process, and to its use for the production of organic compounds. The process according to the invention comprises: a) fractionating dry milling of maize kernels, where the maize kernels are separated into a maize-starch-comprising endosperm fraction and a high-oil germ fraction and, if appropriate, a bran fraction; b) enzymatic liquefaction and saccharification of the maize starch in an aqueous suspension of the endosperm fraction, which gives an aqueous glucose solution comprising maize gluten; and c) depletion of the maize gluten and, if appropriate, any bran present from the aqueous glucose solution.Type: ApplicationFiled: July 4, 2008Publication date: August 5, 2010Applicant: BASF SEInventors: Matthias Boy, Jong-Kyu Choi, Jin Won Chung, Markus Lohscheidt, Jong In Choi, Jai Yeol Seo, Jörg Braun, Mo Se Kim, Sung-Hyun Kim, Arno Kochner
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Publication number: 20100190224Abstract: The present invention relates to an isolated DNA, which is selected from the following sequences: a) a sequence according to SEQ ID No. 01, b) an intron-free sequence that is derived from a sequence according to a) and encodes the same protein or peptide as the sequence according to SEQ ID No. 01, c) a sequence that encodes a protein or peptide, which comprises the amino acid sequence according to SEQ ID No. 02, d) a sequence that is at least 80% identical to a sequence according to a) to c), e) a sequence that hybridizes with the antisense strand of a sequence according to one of the groups a) to d) or would hybridize taking into account degeneration of the genetic code, f) a derivative of a sequence according to one of the groups a) to e) obtained by substitution, addition, inversion and/or deletion of one or more bases, g) a sequence that corresponds to SEQ ID No. 01 within the degeneration of the genetic code, h) a sequence with neutral sense mutations of SEQ ID No.Type: ApplicationFiled: March 27, 2008Publication date: July 29, 2010Applicants: EVONIK DEGUSSA Gmbh, ALBERT-LUDWIG-UNIVERSITAET FREIBURGInventors: Markus Poetter, Achim Marx, Georg Fuchs, Birgit Alber, Tobias Juergen Erb
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Publication number: 20100184172Abstract: A process for the production of natural ferulic acid, coniferyl alcohol and/or vanillin, includes the bio-conversion of eugenol by a bacteria belonging to the Streptomyces genes including at least one nucleotide sequence SEQ ID NO:1 or SEQ ID NO:8 or any nucleotide sequence having at least 70%, preferably 80% and very preferably 90%, identity with the sequence SEQ ID NO:1 or SEQ ID NO:8.Type: ApplicationFiled: February 20, 2008Publication date: July 22, 2010Applicant: V. MANE FILSInventors: Fanny Lambert, Joseph Zucca, Jean Mane
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Publication number: 20100173371Abstract: The present invention relates to a medium composition comprising fermented Korean hot pepper paste, undiluted solution of brewed soy sauce or acid hydrolyzed soy sauce, and a method for producing gamma-aminobutyric acid (GABA) using the medium. More precisely, the present invention relates to a medium composition for culturing lactobacillus having GAD activity, the composition comprising fermented hot pepper paste, brewed soy sauce or acid hydrolyzed soy sauce, and a method for producing a high concentration of gamma-aminobutyric acid from the medium additionally added with glutamic acid or glutamate. The present invention provides a method of producing a high concentration of gamma-aminobutyric acid using the above medium with low production costs. Since the by-products of pastes are used as medium compositions, this medium itself can be added to other pastes or foods to produce functional foods containing gamma-aminobutyric acid.Type: ApplicationFiled: December 22, 2006Publication date: July 8, 2010Applicant: CJ CHEILJEDANG CORPORATIONInventors: Myoung-hee Jeon, Seung-jin Lee, Byoung-koo Kwon, Yeong-il Chang, Hee-kyoung Park, Jun-bong Choi
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Publication number: 20100159545Abstract: The present invention relates to a method of producing corosolic acid by using plant cells that produce corosolic acid. More particularly, the present invention relates to a method of producing corosolic acid by using plant cell suspension cultures comprising the steps of: inducing a callus from a tissue of a plant producing corosolic acid; preparing a cell line capable of being cultured in liquid culture medium from the induced callus; culturing the cell line in a suspension culture; and isolating corosolic acid from the culture solution. The present invention has advantage of maximizing productivity by utilizing two-stage culture, treatment with inducing agent, and high cell-density culture in the suspension culture of plant cells producing corosolic acid.Type: ApplicationFiled: June 30, 2006Publication date: June 24, 2010Applicant: SAMYANG GENEX CORPORATIONInventors: Chang-Heon Kim, Jin-An Kim, Jai-Young Song, Ho-Joon Choi
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Patent number: 7741083Abstract: A process is provided for producing glycolic acid from formaldehyde and hydrogen cyanide. More specifically, heat-treated formaldehyde and hydrogen cyanide are reacted to produce glycolonitrile having low concentrations of impurities. The glycolonitrile is subsequently converted to an aqueous solution of ammonium glycolate using an enzyme catalyst having nitrilase activity derived from Acidovorax facilis 72W (ATCC 57746). Glycolic acid is recovered in the form of the acid or salt from the aqueous ammonium glycolate solution using a variety of methods described herein.Type: GrantFiled: August 12, 2008Date of Patent: June 22, 2010Assignee: E. I. du Pont de Nemours and CompanyInventors: Robert DiCosimo, Anna Panova, Jeffery Scott Thompson, Robert D. Fallon, F. Glenn Gallagher, Thomas Foo, Xu Li, George C. Fox, Joseph J. Zaher, Mark S. Payne, Daniel P. O'Keefe
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Publication number: 20100150891Abstract: It is intended to provide a safe composition for treating a disease or condition relating to autonomic nervous activity which has an autonomic nervous control effect; in particular, an effect of enhancing parasympathetic nervous activity. Thus, a medicinal composition, a food or a drink containing L-phenyl lactic acid and/or D-phenyl lactic acid and having an autonomic nervous control effect is provided.Type: ApplicationFiled: March 28, 2008Publication date: June 17, 2010Applicant: SUNTORY HOLDINGS LIMITEDInventors: Yoshinori Beppu, Nobuo Tsuruoka, Hajime Komura, Katsuya Nagai
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Publication number: 20100143989Abstract: This invention relates to nitrilase mutants having improved nitrilase activity for converting 3-hydroxynitriles to 3-hydroxycarboxylic acids. More specifically, the Acidavorax facilis 72W (ATCC 55746) nitrilase gene was mutated using error-prone PCR and site-directed mutagenesis to create nitrilase enzymes having improved nitrilase activity for converting 3-hydroxynitriles (e.g., 3-hydroxybutyronitrile or 3-hydroxyvaleronitrile) to the corresponding 3-hydroxycarboxylic acids. A process using these improved mutants to produce the 3-hydroxycarboxylic acids is also provided.Type: ApplicationFiled: April 14, 2008Publication date: June 10, 2010Applicant: E. I. DU PONT DE NEMOURS AND COMPANYInventors: Mark S. Payne, Robert DiCosimo, Daniel P. O'Keefe
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Patent number: 7732172Abstract: A process is provided for producing glycolic acid from formaldehyde and hydrogen cyanide. More specifically, heat-treated formaldehyde and hydrogen cyanide are reacted to produce glycolonitrile having low concentrations of impurities. The glycolonitrile is subsequently converted to an aqueous solution of ammonium glycolate using an enzyme catalyst having nitrilase activity derived from Acidovorax facilis 72W (ATCC 57746). Glycolic acid is recovered in the form of the acid or salt from the aqueous ammonium glycolate solution using a variety of methods described herein.Type: GrantFiled: August 12, 2008Date of Patent: June 8, 2010Assignee: E. I. du Pont de Nemours and CompanyInventors: Robert DiCosimo, Anna Panova, Jeffrey Scott Thompson, Robert D. Fallon, F. Glenn Gallagher, Thomas Foo, Xu Li, George C. Fox, Joseph J. Zaher, Mark S. Payne, Daniel P. O'Keefe
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Publication number: 20100112647Abstract: An acidic substance having a carboxyl group is produced by culturing in a medium a microorganism which has been modified to enhance expression of the ybjL gene, and collecting the acidic substance having a carboxyl group from the medium.Type: ApplicationFiled: October 15, 2009Publication date: May 6, 2010Inventors: Yoshihiko Hara, Keita Fukui, Yoshinori Tajima, Kazue Kawamura, Yoshihiro Usuda, Kazuhiko Matsui
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Publication number: 20100068773Abstract: The present invention relates to a cell which has been modified in comparison with its wild type in such a way that it is capable of forming more, by comparison with its wild, 3-hydroxyisobutyric acid or poly-hydroxyalkanoates based on 3-hydroxyisobutyric acid via methylmalonate-semialdehyde or 3-hydroxybutyryl-coenzyme A as precursors. The invention also relates to a method of generating a genetically modified cell, to the genetically modified cell obtainable by these methods, to a method of producing 3-hydroxyisobutyric acid or polyhydroxyalkanoates based on 3-hydroxyisobutyric acid, to a method of producing methacrylic acid or methacrylic esters, and to a method of producing polymethacrylic acid or polymethacrylic esters. The present invention furthermore relates to an isolated DNA, to a vector, to the use of this vector for transforming a cell, to a transformed cell, and to a polypeptide.Type: ApplicationFiled: June 1, 2007Publication date: March 18, 2010Applicant: Evonik Roehm GmbHInventors: Achim Marx, Markus Poetter, Stefen Buchholz, Alexander May, Hermann Siegert, Georg Fuchs, Birgit Alber, Lothar Eggeling
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Publication number: 20100035314Abstract: The present invention relates to a method for the enzymatic production of 2-hydroxy-2-methyl carboxylic acids from 3-hydroxy carboxylic acids, where a 3-hydroxy carboxylic acid is produced in an aqueous reaction solution and/or is added to this reaction solution and is incubated. The aqueous reaction solution comprises a unit having 3-hydroxy-carboxylate-CoA mutase activity which has both 3-hydroxy-carbonyl-CoA ester-producing and 3-hydroxy-carbonyl-CoA ester-isomerizing activity and converts the 3-hydroxy carboxylic acid into the corresponding 2-hydroxy-2-methyl carboxylic acid which is isolated as acid or in the form of its salts. In a preferred embodiment, the unit having 3-hydroxy-carboxylate-CoA mutase activity is a unit which includes an isolated cobalamin-dependent mutase and where appropriate a 3-hydroxy-carbonyl-CoA ester-producing enzyme or enzyme system or a microorganism including them.Type: ApplicationFiled: March 23, 2007Publication date: February 11, 2010Applicant: Evonik Roehm GmbHInventors: Roland H. Mueller, Thore Rohwerder
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Publication number: 20100021978Abstract: A non-naturally occurring microbial organism having a 3-hydroxypropanoic acid (3-HP) pathway includes at least one exogenous nucleic acid encoding 3-HP pathway enzyme expressed in a sufficient amount to produce 3-HP. The 3-HP pathway includes a 2-keto acid decarboxylase, a CoA-dependent oxaloacetate dehydrogenase, or a malate decarboxylase. A method for producing 3-HP includes culturing a non-naturally occurring microbial organism having a 3-HP pathway that includes at least one exogenous nucleic acid encoding a 3-HP pathway enzyme expressed in a sufficient amount to produce 3-HP under conditions and for a sufficient period of time to produce 3-HP. The 3-HP pathway includes a 2-keto acid decarboxylase, a CoA-dependent oxaloacetate dehydrogenase, or a malate decarboxylase.Type: ApplicationFiled: July 23, 2009Publication date: January 28, 2010Applicant: GENOMATICA, INC.Inventors: Mark J. Burk, Robin E. Osterhout
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Publication number: 20100015675Abstract: A process for the production of a fluorine containing ?-hydroxy carboxylic acid by hydrolyzing a fluorine containing ?-hydroxy nitrite in the presence of a nitrilase.Type: ApplicationFiled: July 18, 2007Publication date: January 21, 2010Applicant: SOLVAY FLUOR GMBHInventors: Max Braun, Valérie Lejeune, Véronique Mathieu
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Publication number: 20090325249Abstract: A process is provided to improve the specific activity of an enzyme catalyst having nitrilase activity when converting glycolonitrile to glycolic acid under aqueous reaction conditions. Inclusion of an effective amount of at least one amine protectant improves the specific activity and catalytic productivity of the enzyme catalyst.Type: ApplicationFiled: April 24, 2009Publication date: December 31, 2009Inventors: ROBERT DICOSIMO, ANNA PANOVA, HENRY KEITH CHENAULT, SAMUEL D. ARTHUR
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Publication number: 20090325250Abstract: A process is provided to improve the specific activity of an enzyme catalyst having nitrilase activity when converting glycolonitrile to glycolic acid under aqueous reaction conditions. Inclusion of an effective amount of at least one amine protectant improves the specific activity and catalytic productivity of the enzyme catalyst.Type: ApplicationFiled: April 24, 2009Publication date: December 31, 2009Inventors: ROBERT DICOSIMO, Anna Panova, Henry Keith Chenault, Samuel D. Arthur
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Publication number: 20090312425Abstract: A composition containing 1,4-dihydroxy-2-naphthoic acid at a high concentration is obtained by intracellularly and extracellularly producing 1,4-dihydroxy-2-naphthoic acid using a bacterium belonging to the genus Propionibacterium and collecting it. This composition is efficacious in improving intestinal flora, alleviating abdominal ailments in association with the intake of milk, and preventing metabolic bone diseases.Type: ApplicationFiled: August 19, 2009Publication date: December 17, 2009Applicant: MEIJI DAIRIES CORPORATIONInventors: Yoshio Sato, Seiya Makino, Nobuo Yoda, Kakuhei Isawa, Tomonori Kamiyama, Kenichi Hojo, Mizue Saito, Naoki Taketomo, Keisuke Furuichi, Shuji Ikegami
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Publication number: 20090305368Abstract: Hydroxycarboxylic acids are produced by using a microorganism that is improved in ability to regenerate oxidized-type nicotinamide adenine dinucleotide by being provided with an enhanced NADH dehydrogenase function by introducing a gene encoding NADH dehydrogenase into a microorganism.Type: ApplicationFiled: April 27, 2007Publication date: December 10, 2009Applicant: Mitsui Chemicals Inc.Inventors: Takashi Morishige, Mitsufumi Wada, Hitoshi Takahashi, Daisuke Mochizuki, Junko Tokuda
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Patent number: 7629157Abstract: The present invention is directed to variant polypeptides having enhanced ketoreductase activity and/or thermostability for use in the stereospecific reduction of ketones. In addition, the present invention is directed to polynucleotides that encode the ketoreductase polypeptides, including codon optimized versions of the polynucleotides which provide for enhanced expression in host cells. In another aspect, the present invention is directed to nucleotide constructs, vectors and host cells that are transformed with polynucleotides of the present invention.Type: GrantFiled: August 11, 2004Date of Patent: December 8, 2009Assignee: Codexis, Inc.Inventors: S. Christopher Davis, Gjalt W. Huisman, Stephane J. Jenne, Anke Kebber, Lisa Marie Newman
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Publication number: 20090239249Abstract: The present invention relates to a novel thermostable gluconate dehydratase from the thermoacidophilic archaeon Sulfolobus solfataricus, a coding sequence, and an expression system. The gluconate dehydratase has a molecular weight of about 320,000 to 380,000 daltons as the native protein, and about 40,000 to 50,000 daltons as the monomer protein, and catalyzes the dehydration reaction of aldonic acids to 2-keto-3-deoxy derivatives at temperatures of less than 120° C. The gluconate dehydratase can be produced from native or recombinant host cells and thereby used in the pharmaceutical, agricultural, and other industries.Type: ApplicationFiled: May 13, 2004Publication date: September 24, 2009Applicants: POSCO, POSTECH FoundationInventors: Seonghun Kim, Sun Bok Lee
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Publication number: 20090233287Abstract: The present invention provides a recombinant Penicillium chrysogenum strain characterized in that a gene selected from the list consisting of penDE, pcbAB and pcbC is inactivated and the use of such a strain for the preparation of a compound of interest.Type: ApplicationFiled: April 25, 2007Publication date: September 17, 2009Inventors: Roelof Ary Lans Bovenberg, Marco Alexander Van den Berg, Susanne Hage, Paul Klaassen, Bernard Meijrink, Lourina Madeleine Raamsdonk
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Publication number: 20090233339Abstract: A process is provided to improve the specific activity of an enzyme catalyst having nitrilase activity when converting glycolonitrile to glycolic acid under aqueous reaction conditions. Inclusion of an effective amount of at least one amine protectant improves the specific activity and catalytic productivity of the enzyme catalyst.Type: ApplicationFiled: April 24, 2009Publication date: September 17, 2009Inventors: ROBERT DICOSIMO, ANNA PANOVA, HENRY KEITH CHENAULT, SAMUEL DAVID ARTHUR
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Publication number: 20090226571Abstract: The present invention relates to a method for the production of at least one nonvolatile microbial metabolite in solid form by sugar-based microbial fermentation, in which process a microorganism strain which produces the desired metabolites is grown using a sugar-containing liquid medium with a monosaccharide content of more than 20% by weight based on the total weight of the liquid medium, and the volatile constituents of the fermentation liquor are subsequently largely removed, the sugar-containing liquid medium being prepared by: a1) milling selected starch feedstock from cereal grains; and a2) liquefying the millbase in an aqueous liquid in the presence of at least one starch-liquefying enzyme, followed by saccharification using at least one saccharifying enzyme, where, for liquefaction purposes, at least a portion of the millbase is liquefied by continuous or batchwise addition to the aqueous liquid.Type: ApplicationFiled: September 6, 2006Publication date: September 10, 2009Applicant: BASF SEInventors: Stephan Freyer, Markus Pompejus, Oskar Zelder, Markus Lohscheidt, Matthias Boy, Edzard Scholten
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Publication number: 20090221045Abstract: Hydroxycarboxylic acids are produced by using a microorganism that is improved in ability to produce nicotinamide adenine dinucleotide by deleting, mutating or substituting nadR gene in the microorganism or introducing a gene encoding nicotinic acid phosphoribosyltransferase.Type: ApplicationFiled: April 27, 2007Publication date: September 3, 2009Applicant: MITSUI CHEMICALS, INC.Inventors: Takashi Morishige, Mitsufumi Wada, Hitoshi Takahashi, Daisuke Mochizuki, Junko Tokuda
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Patent number: 7582215Abstract: A process of separating suspended solids from a fermentation liquor by subjecting the liquor to a solids-liquid separation stage, wherein the fermentation liquor is produced in a fermentation process for the production of a fermentation product, which liquor comprises water, lignin and BOD, wherein the solids liquid separation stage is assisted by a treatment system, characterised in that the treatment system comprises either, (i) a cationic polymer having an intrinsic viscosity (IV) of at least 4 dl/g at a dose of above 2 kg/tonne based on dry weight of suspension, or (ii) a cationic polymer having an intrinsic viscosity (IV) of at least 4 dl/g and, (iii) an anionic polymer, and/or (iv) a cationic polymer of intrinsic viscosity of below 4 dl/g and a cationic charge density of at least 3 meq/g and/or (v) inorganic coagulants and/or (vi) charged microparticulate material.Type: GrantFiled: July 30, 2003Date of Patent: September 1, 2009Assignee: Ciba Specialty Chemicals Water Treatments Ltd.Inventors: Jonathan Hughes, Kenneth Charles Symes
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Patent number: 7579176Abstract: Embodiments of the invention relate to the microbial production of polyhydroxyalkanoic acids, or polyhydroxyalkanoates (PHA), from substrates which cannot be used as a source of carbon and/or energy for microbial growth or PHA synthesis and which have microbial and environmental toxicity. According to one embodiment of the invention, a process for the production of PHA is provided wherein an enzyme such as methane monooxygenase is used to convert volatile organic compounds into PHA through the use of microorganisms that are unable to use volatile organic compounds as a source of carbon for growth or PHA production.Type: GrantFiled: February 20, 2007Date of Patent: August 25, 2009Assignee: Newlight Technologies, LLCInventors: Markus Herrema, Kenton Kimmel
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Patent number: 7575909Abstract: The invention relates to a NADH-dependent oxidoreductase obtained from yeasts, for example, of the genus Pichia, more particularly from Pichia capsulata. The oxidoreductace used in an enzymatic process for enantioselective reduction of organic ketocompounds to the corresponding (S)-hydroxy compounds and in an enzymnatic process for an antioselective preparation of (S)-hydroxy compounds in a two phase system using the isolated, recombinantly overexpressed oxidoreductacse from Pichia capsulata.Type: GrantFiled: May 28, 2004Date of Patent: August 18, 2009Assignee: IEP GmbHInventors: Antje Gupta, Anke Tschentscher, Maria Bobkova
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Patent number: 7572612Abstract: A thermostable TAL enzyme was identified from the fungus Phanerochaete chrysosporium, which has high activity at temperatures of 40° C. to about 60° C. The enzyme was produced in engineered cells and used for production of para-hydroxycinnamic acid (pHCA) from tyrosine. When the pHCA production reaction was run at high temperature, the enzyme was more active and pHCA was produced more rapidly, making the reaction more efficient.Type: GrantFiled: July 12, 2006Date of Patent: August 11, 2009Assignee: E.I. du Pont de Nemours and CompanyInventors: Lixuan Lisa Huang, Zhixiong Xue, Michael P. McCluskey
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Publication number: 20090162906Abstract: The invention provides nitrilases and methods for making and using them, and in one aspect, provides methods for producing enantiomerically pure ?-substituted carboxylic acids, such as, for example, ?-amino acids and ?-hydroxy acids. In one aspect, methods of the invention combine an aldehyde or ketone with a cyanide and ammonia or an ammonium salt or an amine, in the presence of a nitrilase or a polypeptide having nitrilase activity, to stereoselectively hydrolyze the amino nitrile or cyanohydrin intermediate under conditions sufficient to produce the carboxylic acid.Type: ApplicationFiled: October 8, 2007Publication date: June 25, 2009Applicant: Verenium CorporationInventors: Mark MADDEN, David Paul Weiner, Jennifer Ann Chaplin
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Publication number: 20090162892Abstract: The invention relates to a process for the production of at least one microbial metabolite having at least 3 carbon atoms or at least 2 carbon atoms and at least 1 nitrogen atom by means of sugar-based microbial fermentation, comprising: a) the preparation of a sugar-containing liquid medium with a monosaccharide content of more than 20% by weight from a starch feedstock, the sugar-containing liquid medium also comprising non-starchy solid constituents of the starch feedstock; b) the fermentation of the sugar-containing liquid medium for the production of the metabolite(s); and c) depletion or isolation of at least one metabolite from the fermentation liquor, wherein a microorganism strain which produces the desired metabolite(s) is cultivated with the sugar-containing liquid medium, said liquid medium being obtained by: a1) milling the starch feedstock; and a2) liquefying the millbase in an aqueous liquid in the presence of at least one starch-liquefying enzyme, followed by saccharification using at leaType: ApplicationFiled: May 27, 2005Publication date: June 25, 2009Applicant: BASF AGInventors: Markus Pompejus, Stephan Freyer, Markus Lohscheidt, Oskar Zelder, Matthias Boy
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Publication number: 20090155867Abstract: The present invention provides a method for the biological production of glycolic acid from a fermentable carbon source in a microorganism. In one aspect of the present invention, a process for the conversion of glucose to glycolic acid is achieved by the use of a recombinant organism comprising a host E. coli transformed i) to attenuate the glyoxylate consuming pathways to other compounds than glycolate ii) to use an NADPH glyoxylate reductase to convert glyoxylate to glycolate iii) to attenuate the level of all the glycolate metabolizing enzymes and iv) increase the flux in the glyoxylate pathway. In another aspect of the present invention, the process for the production of glycolic acid from a fermentable carbon source, using a recombinant E. coli, is improved by increasing the NADPH availability in the cells.Type: ApplicationFiled: June 7, 2007Publication date: June 18, 2009Inventor: Philippe Soucaille
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Publication number: 20090118541Abstract: Provided is a production method of glycolic acid having a first step of preparing glycolonitrile from formaldehyde and hydrocyanic acid and a second step of hydrolyzing the glycolonitrile into glycolic acid directly or via a glycolate salt, which method can produce glycolic acid in easy production and purification steps while consuming less energy. In the production method, by carrying out the first and second steps continuously or by storing the glycolonitrile obtained in the first step at pH 4 or less and carrying out a hydrolysis reaction of the second step at from pH 5 to 9, a production yield of glycolic acid, activity for the production of glycolic acid and accumulated concentration of glycolic acid are improved, resulting in the production of glycolic acid having an improved purity and quality.Type: ApplicationFiled: May 25, 2006Publication date: May 7, 2009Applicant: ASAHI KASEI CHEMICALS CORPORATIONInventors: Hidenori Hinago, Hajime Nagahara, Toshiya Aoki
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Patent number: 7517675Abstract: A method of oxidising carbohydrates and/or carbohydrate derivatives having primary alcohol groups comprising contacting a reaction medium containing said carbohydrates and/or carbohydrate derivatives with a nitroxy radical mediator and a peroxidase enzyme, characterized in that the initial reaction medium contains at least 10% by weight carbohydrates and/or carbohydrate derivatives, in that the peroxidase enzyme is an oilseed peroxidase and in that a hydroperoxide and an alkali compound are gradually added to the reaction medium such that its pH is maintained at between 3.5 and 10.0 (pH-Stat); use of said method in a process for producing gluconic acid, glucaric acid and/or D-glucuronolactone.Type: GrantFiled: December 17, 2004Date of Patent: April 14, 2009Assignee: Cerestar Holding B.V.Inventors: Ronny Leontina Marcel Vercauteren, An Amanda Jules Heylen
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Method for producing chiral ?-hydroxycarboxylic acids by enzymatic hydrolysis of chiral cyanohydrins
Patent number: 7514245Abstract: The invention relates to a method for producing chiral ?-hydroxycarboxylic crystalline acids consisting in transforming cyanhydrins (R) or (S) into ?-hydroxycarboxylic acids (R) or (S), respectively by enzymatic hydrolysis in the presence of Rhodococcus erythropolis NCIMB 11540.Type: GrantFiled: September 9, 2005Date of Patent: April 7, 2009Assignee: DSM Fine Chemicals Austria NFG GmbH & Co. KGInventors: Herfried Griengl, Ingrid Osprian, Hans Schoemaker, Christoph Reisinger, Helmut Schwab