Butanol Patents (Class 435/160)
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Publication number: 20130217078Abstract: The present invention relates to isolated polypeptides having cellulolytic enhancing activity and isolated polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides.Type: ApplicationFiled: April 4, 2013Publication date: August 22, 2013Applicants: Novozymes, Inc., Novozymes A/SInventors: Novozymes A/S, Novozymes, Inc.
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Publication number: 20130217075Abstract: In a method of producing biofuel using brown algae, Bacterium antarctica is used as a hydrolysis catalyst for saccharification to obtain monosaccharides from the brown algae. The saccharification with the hydrolysis catalyst is effective in saccharification of the brown algae.Type: ApplicationFiled: February 12, 2013Publication date: August 22, 2013Applicant: SAMSUNG ELECTRONICS CO., LTDInventors: Hwa Young CHO, Byung Jo YU, Jae Chan PARK, Sung Min PARK, Jae Hwa LEE
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Publication number: 20130217079Abstract: The present invention relates to variants of a parent beta-glucosidase. The present invention also relates to polynucleotides encoding the variants; nucleic acid constructs, vectors, and host cells comprising the polynucleotides; and methods of using the variants.Type: ApplicationFiled: September 30, 2011Publication date: August 22, 2013Applicant: Novozymes, Inc.Inventors: Mark Wogulis, Paul Harris, David Osborn
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Publication number: 20130210096Abstract: Methods and systems for the production of hydrocarbon products, including providing a substrate comprising CO to a bioreactor containing a culture of one or more micro-organisms; and fermenting the culture in the bioreactor to produce one or more hydrocarbon products. The substrate comprising CO is derived from an industrial process selected from the group comprising steam reforming processes, refinery processes, steam cracking processes, and reverse water gas shift processes.Type: ApplicationFiled: October 21, 2011Publication date: August 15, 2013Applicant: LANZATECH NEW ZEALAND LIMITEDInventors: Michael Anthony Schultz, James Obern, Sean Dennis Simpson
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Publication number: 20130211143Abstract: The present invention relates to a cell retaining matrix (CRB) for producing solvents and alcohols and an apparatus comprising said bio-column. The invention also relates to the method for producing solvents and alcohols by using said cell retaining matrix (CRB). Said cell retaining biomatrix (CRB cellulosic fibers, selected from the group consisting wood cellulosic fibers, pulp cellulosic fibers, vegetable cellulosic fibers, such as mechanical pulp, dissolving pulp, lignocellulosic fibers, and cellulosic fibers originated from vegetable peels, and at microbes, which have been immobilized into said cellulosic fibers, and which can save their biological activity in cell retaining biomatrix (CRB).Type: ApplicationFiled: July 8, 2011Publication date: August 15, 2013Applicant: AALTO UNIVERSITY FOUNDATIONInventor: Tom Granstrom
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Publication number: 20130210087Abstract: The present invention provides isolated polypeptides having cellobiohydrolase activity and isolated polynucleotides encoding the polypeptides. The invention also provides nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides.Type: ApplicationFiled: November 4, 2011Publication date: August 15, 2013Applicant: NOVOZYMES INC.Inventors: Ye Liu, Lan Tang
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Publication number: 20130210088Abstract: A method is provided for improving enzymatic hydrolysis in saccharification of a lignocellulosic material. The method is comprising pretreating the lignocellulosic material to obtain a slurry of pretreated lignocellulosic material; adding at least one reducing agent to the slurry of pretreated lignocellulosic material or the liquid fraction thereof to decrease the enzymatic hydrolysis inhibitory properties of slurry of the pretreated lignocellulosic material or the liquid fraction thereof; and subjecting the slurry of pretreated lignocellulosic material or the liquid fraction thereof to enzymatic hydrolysis in the presence of the at least one reducing agent.Type: ApplicationFiled: October 6, 2010Publication date: August 15, 2013Inventors: Bjorn Alriksson, Lelf Jonsson, Venkata Prabhakar Soudham
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Publication number: 20130203138Abstract: Provided herein are polypeptides and polynucleotides encoding such polypeptides which have ketoisovalerate decarboxylase activity. Also provided are recombinant host cells comprising such polypeptides and polynucleotides and methods of use thereof.Type: ApplicationFiled: July 27, 2012Publication date: August 8, 2013Applicant: BUTAMAX(TM) ADVANCED BIOFUELS LLCInventors: Jessica McElvain, Daniel P. Okeefe, Brian James Paul, Mark S. Payne, Steven Cary Rothman, Hongxian He
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Publication number: 20130196387Abstract: The present invention provides isolated polypeptides having endoglucanase activity and isolated polynucleotides encoding the polypeptides. The invention also provides nucleic acid constructs, vectors, and host cell comprising the polynucleotides as well as methods of producing and using the polypeptides.Type: ApplicationFiled: November 11, 2011Publication date: August 1, 2013Applicant: Novozymes Inc.Inventors: Ye Liu, Junxin Duan, Lan Tang
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Publication number: 20130196398Abstract: A method is provided in which pretreated and densified cellulosic biomass particulates can hydrolyzed at a high solids loading rate as compared with the solids loading rate of loose hydrolysable cellulosic biomass fibers. The resulting high concentration sugar-containing stream can be easily converted to biofuels or an entire suite of other useful bioproducts.Type: ApplicationFiled: April 27, 2012Publication date: August 1, 2013Inventors: Bryan Bals, Farzaneh Teymouri, Tim Campbell, Bruce Dale
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Publication number: 20130196386Abstract: Biomass (e.g., plant biomass, animal biomass, and municipal waste biomass) is processed to produce useful products, such as fuels. For example, systems are described that can use feedstock materials, such as cellulosic and/or lignocellulosic materials, to produce ethanol and/or butanol, e.g., by fermentation.Type: ApplicationFiled: March 13, 2013Publication date: August 1, 2013Applicant: XYLECO, INC.Inventor: XYLECO, INC.
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Patent number: 8497105Abstract: Processes and systems for production of bioproducts such as biofuels are provided. The bioproduct production processes and systems utilize pretreatment of a carbohydrate-containing feedstock to produce soluble sugar molecules and continuous conversion of the pretreated feedstock to a bioproduct by an immobilized fermenting microorganism.Type: GrantFiled: June 24, 2010Date of Patent: July 30, 2013Assignee: Cobalt Technologies, Inc.Inventors: David C. Walther, Hendrik J. Meerman, Stacy M. Burns-Guydish, Richard W. Wilson, Eamon T. Hogg, Gregory W. Luli, Robert Eckert
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Publication number: 20130189734Abstract: The present invention provides isolated polypeptides having cellobiohydrolase activity and isolated polynucleotides encoding the polypeptides. The invention also provides nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides.Type: ApplicationFiled: November 2, 2011Publication date: July 25, 2013Applicant: NOVOZYMES INC.Inventors: Ye Liu, Lan Tang, Junxin Duan
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Publication number: 20130189750Abstract: Methods and systems to achieve clean fuel processing systems in which carbon dioxide emissions (1) from sources (2) may be processed in at least one processing reactor (4) containing a plurality of chemoautotrophic bacteria (5) which can convert the carbon dioxide emissions into biomass (6) which may then be used for various products (21) such as biofuels, fertilizer, feedstock, or the like. Sulfate reducing bacteria (13) may be used to supply sulfur containing compounds to the chemoautotrophic bacteria (5).Type: ApplicationFiled: March 10, 2013Publication date: July 25, 2013Applicant: The University of Wyoming Research d/b/a Western Research InstituteInventor: The University of Wyoming Research d/b/a Western Research Institute
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Publication number: 20130189724Abstract: A carbonaceous feedstock can be converted into a chemical product having a higher commercial value, by introducing the carbonaceous feedstock into a plasma torch under conditions selected to generate a synthetic gas mixture having a tailored composition, and then introducing the synthetic gas mixture into a microbial digester configured to convert the synthetic gas mixture into the chemical product. The composition of the synthetic gas mixture produced by the plasma torch can be quickly modified to yield an optimal quality and quantity required by the microbial digester, and the quantity and stoichiometric ratio can be quickly varied if the quantity or composition of the synthetic gas mixture being provided is not appropriate for the microbial digester, enabling greater efficiency to be achieved as compared to systems where the quality and quantity of the synthetic gas mixture cannot be easily changed.Type: ApplicationFiled: March 8, 2013Publication date: July 25, 2013Applicant: C-TECH LLCInventor: C-Tech LLC
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Publication number: 20130189747Abstract: The present invention relates to compositions comprising: a polypeptide having cellulolytic enhancing activity and a sulfur-containing compound. The present invention also relates to methods of using the compositions.Type: ApplicationFiled: August 5, 2011Publication date: July 25, 2013Applicant: NOVOZYMES, INC.Inventor: Feng Xu
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Patent number: 8492127Abstract: The invention is concerned with the strains of B. coagulans for lactic acid production and the related methods, in which the carbon sources are pentose or hexose or the agricultural or industrial wastes containing pentose or hexose or a mixture of both. According to the invention, the highest amount of L-lactic acid produced from glucose is 173 g/L, the optical purity is over 99%, the yield is up to 0.98, and the productivity is up to 2.4 g/L per hour. The highest amount of L-lactic acid produced from xylose is 195 g/L, the optical purity is over 99%, the yield is up to 0.98, and the productivity is up to 2.7 g/L per hour. The highest amount of L-lactic acid produced from reducing sugars in xylitol byproducts is 106 g/L, the optical purity is over 99%, and the productivity is up to 2.08 g/L per hour. The B. coagulans strains XZL4 (DSM No. 23183) and XZL9 (DSM No.Type: GrantFiled: November 22, 2010Date of Patent: July 23, 2013Assignee: Shanghai Jiao Tong UniversityInventors: Ping Xu, Limin Wang, Bo Zhao, Cuiqing Ma, Fei Su, Fei Tao, Hongzhi Tang
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Publication number: 20130180258Abstract: The subject of the invention is a process for producing an oxygen-containing compound chosen from C1 to C4 alcohols or carboxylic acids, and mixtures thereof, comprising the following steps: a step of catalytic decomposition of a hydrocarbon-based feedstock to give carbon, said step also producing hydrogen; the partial oxidation of the resulting carbon to give CO, using CO2, the CO stream being separated from the hydrogen stream; and the conversion of the resulting CO to give an oxygen-containing compound by anaerobic fermentation in an aqueous medium, the fermentation also producing CO2, the partial oxidation step being carried out with the CO2 produced during the fermentation.Type: ApplicationFiled: September 26, 2011Publication date: July 18, 2013Applicant: TOTAL RAFFINAGE CHIMIEInventor: Luis Angel Robels Macias
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Publication number: 20130183730Abstract: This invention is directed to methods for recovery of C3-C6 alcohols from dilute aqueous solutions, such as fermentation broths. Such methods provide improved volumetric productivity for the fermentation and allows recovery of the alcohol. Such methods also allow for reduced energy use in the production and drying of spent fermentation broth due to increased effective concentration of the alcohol product by the simultaneous fermentation and recovery process which increases the quantity of alcohol produced and recovered per quantity of fermentation broth dried. Thus, the invention allows for production and recovery of C3-C6 alcohols at low capital and reduced operating costs.Type: ApplicationFiled: November 22, 2011Publication date: July 18, 2013Applicant: GENO, INC.Inventors: William A. Evanko, Aharon M. Eyal, David A. Glassner, Fudu Miao, Aristos A. Aristidou, Kent Evans, Patrick R. Gruber, Andrew C. Hawkins, Peter Meinhold, Reid M. Renny Feldman, Uvini Gunawardena, Jun Urano
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Publication number: 20130183731Abstract: Methods for the fermentative production of four carbon alcohols is provided. Specifically, butanol, preferably isobutanol is produced by the fermentative growth of a recombinant bacterium expressing an isobutanol biosynthetic pathway.Type: ApplicationFiled: October 5, 2012Publication date: July 18, 2013Applicant: BUTAMAX (TM) ADVANCED BIOFUELS LLCInventor: BUTAMAX (TM) ADVANCED BIOFUELS LLC
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Publication number: 20130183721Abstract: Systems and methods for cooling and processing materials are disclosed.Type: ApplicationFiled: March 6, 2013Publication date: July 18, 2013Applicant: XYLECO, INC.Inventor: XYLECO, INC.
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Publication number: 20130183713Abstract: The present invention relates to isolated polypeptides having beta-glucosidase activity, beta-xylosidase activity, or beta-glucosidase and beta-xylosidase activity and isolated polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides.Type: ApplicationFiled: August 30, 2011Publication date: July 18, 2013Applicants: NOVOYZMES A/S, NOVOZYMES, INC.Inventor: Marc Morant
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Publication number: 20130177957Abstract: A process that generates sulfide in production of liquid products from syngas and a system for syngas fermentation including a sulfide generation process to convert feed gas to liquid product, the process including: partially oxidizing high-temperature gas, input gas, and molten sulfur to generate sulfide gas, the high-temperature gas containing oxygen, the input gas containing carbonaceous fuel and the sulfide gas containing hydrogen sulfide, CO, CO2, and H2; mixing the sulfide gas with the feed gas to generate mixed gas having a predetermined hydrogen sulfide concentration; providing at least a portion of the mixed gas to a production fermentor containing microorganisms; and converting the mixed gas to the liquid product by contact with the microorganisms.Type: ApplicationFiled: January 6, 2012Publication date: July 11, 2013Inventors: JIANXIN DU, RATHIN DATTA
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Publication number: 20130171706Abstract: Methods for the fermentative production of four carbon alcohols is provided. Specifically, butanol, preferably isobutanol is produced by the fermentative growth of a recombinant bacterium expressing an isobutanol biosynthetic pathway.Type: ApplicationFiled: June 29, 2012Publication date: July 4, 2013Applicant: BUTAMAX ADVANCED BIOFUELS LLCInventors: Gail K. Donaldson, Andrew C. Eliot, Dennis Flint, Lori Ann Maggio-Hall, Vasantha Nagarajan
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Patent number: 8476047Abstract: In an alcohol fermentation process, oil derived from biomass is hydrolyzed into an extractant available for in situ removal of a product alcohol such as butanol from a fermentation broth. The glycerides in the oil can be catalytically (e.g., enzymatically) hydrolyzed into free fatty acids, which form a fermentation product extractant having a partition coefficient for a product alcohol greater than a partition coefficient of the oil of the biomass for the product alcohol. Oil derived from a feedstock of an alcohol fermentation process can be hydrolyzed by contacting the feedstock including the oil with one or more enzymes whereby at least a portion of the oil is hydrolyzed into free fatty acids forming a fermentation product extractant, or the oil can be separated from the feedstock prior to the feedstock being fed to a fermentation vessel, and the separated oil can be contacted with the enzymes to form the fermentation product extractant.Type: GrantFiled: December 3, 2012Date of Patent: July 2, 2013Assignee: Butamax Advanced Biofuels LLCInventors: Keith H. Burlew, Robert Dicosimo, Michael Charles Grady
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Patent number: 8476046Abstract: Disclosed is a method of pre-treating and saccharifying an algae biomass, by dehydrating the algae biomass to have a water content of about 10% to about 70% by weight, cutting the algae biomass having a water content of about 10% to about 70% by weight to a predetermined size, and saccharifying the cut algae biomass using a hydrolysis catalyst and/or a hydrolase to yield a monosaccharide.Type: GrantFiled: June 22, 2009Date of Patent: July 2, 2013Assignee: Samsung Electronics Co., Ltd.Inventors: Hwa Young Cho, Jun Seok Kim, Jae Chan Park, Sung Min Park, Jin Woo Kim
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Publication number: 20130164802Abstract: A moving bed bioreactor (MBBR) produces liquid products from a gaseous substrate of CO and/or CO2/H2 using a biomass that grows on the surface of carrier suspended in a fermentation broth into which the gaseous substrate is at least partially dissolved. The injection devices include gas spargers and preferably a high efficiency gas transfer process such as jet or slot aerator/gas transfer devices. The gas injection device introduces the gas in the form of microbubbles in a substantially horizontal directions that creates eddy currents in the surrounding liquid for thoroughly mixing the fermentation broth in a fermentation vessel. Gas bubbles from the gas delivery device rise through the liquid surface and provide additional mixing and gas dissolution. The motion of gas and liquid keeps the biomass carrier moving can also provide sufficient shear so as to maintain the biofilm thickness on the biomass carrier media in the desirable range.Type: ApplicationFiled: March 1, 2013Publication date: June 27, 2013Inventor: Robert Hickey
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Publication number: 20130164798Abstract: The present invention relates to processes for producing industrial products such as hydrocarbon products from non-polar lipids in a vegetative plant part. Preferred industrial products include alkyl esters which may be blended with petroleum based fuels.Type: ApplicationFiled: December 21, 2012Publication date: June 27, 2013Applicant: Commonwealth Scientific and Industrial Research OrganisationInventors: Thomas Vanhercke, James Robertson Petrie, Anna El Tahchy, Surinder Pal Singh, Qing Liu
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Publication number: 20130164795Abstract: The present invention includes methods of generating co-products for animal feed and compositions useful as co-products for animal feed derived from biofuel production processes. More specifically, the invention includes co-products for animal feed from at least one process feedstream, such as fatty acids from oil hydrolysis, lipids from evaporation of thin stillage, syrup, distillers grains, distillers grains and solubles, solids from a mash before fermentation, and solids from a whole stillage after fermentation.Type: ApplicationFiled: June 15, 2012Publication date: June 27, 2013Applicant: BUTAMAX(TM) ADVANCED BIOFUELS LLCInventors: David J. LOWE, Brian Michael Roesch
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Publication number: 20130164804Abstract: Methods are provided for preparing a hydrolysate containing soluble sugar molecules from biomass that contains cellulose and hemicellulose. Hemicellulose sugars are extracted in the process, and the resulting hydrolysate may be used to support microbial fermentation to produce products of interest.Type: ApplicationFiled: December 20, 2012Publication date: June 27, 2013Inventors: David C. Walther, William F. McDonald
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Patent number: 8470566Abstract: A non-naturally occurring microbial organism having an isopropanol, 4-hydroxybutryate, or 1,4-butanediol pathway includes at least one exogenous nucleic acid encoding an isopropanol, 4-hydroxybutryate, or 1,4-butanediol pathway enzyme expressed in a sufficient amount to produce isopropanol, 4-hydroxybutryate, or 1,4-butanediol. The aforementioned organisms are cultured to produce isopropanol, 4-hydroxybutryate, or 1,4-butanediol.Type: GrantFiled: February 10, 2012Date of Patent: June 25, 2013Assignee: Genomatica, Inc.Inventors: John D. Trawick, Mark J. Burk, Anthony P. Burgard
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Publication number: 20130157307Abstract: The invention relates to a process of fermenting plant material in a fermentation medium into a fermentation product using a fermenting organism, wherein one or more deamidases are present in the fermentation medium.Type: ApplicationFiled: August 2, 2011Publication date: June 20, 2013Applicants: NOVOZYMES NORTH AMERICA, INC., NOVOZYMES A/SInventors: Chee-Leong Soong, Shiro Fukuyama, Allan Noergaard, Preben Nielsen, Peter Rahbek Oestergaard
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Publication number: 20130157314Abstract: The present invention relates to compositions comprising: a polypeptide having cellulolytic enhancing activity and a heterocyclic compound. The present invention also relates to methods of using the compositions.Type: ApplicationFiled: August 5, 2011Publication date: June 20, 2013Applicant: NOVOZYMES, INC.Inventors: Feng Xu, Matthew Sweeney, Jason Quinlan
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Publication number: 20130157326Abstract: Systems and methods are provided for enhancing the integration of processes for recovering products from algae-derived biomass. The enhanced process integration allows for increased use of input streams and other reagents that are derived from renewable sources. This increases the overall renewable character of the products extracted from the algae-derived biomass. The process integration can include exchange of input streams or energy between an algae processing system and a system for processing non-algal biomass. One example of improving process integration is using oxygenates that are generated in a renewable manner as a reagent for enhancing the algae processing system.Type: ApplicationFiled: December 14, 2011Publication date: June 20, 2013Applicant: EXXONMOBIL RESEARCH AND ENGINEERING COMPANYInventors: Paul D. OLDENBURG, Michel DAAGE, Virginia M. ROBERTS, Paul J. BERLOWITZ, David C. LONG, James R. BIELENBERG
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Publication number: 20130149758Abstract: The efficient production of ethanol from low-cost biomass (e.g., corn, sugar beets, sugar cane, switchgrass and/or paper) has become increasingly important in making ethanol competitive with gasoline and decreasing the United States' dependence on foreign oil. For example, to reduce the cost of transporting biomass to ethanol production facilities, mobile systems for producing ethanol from biomass are provided. Also provided are small-scale ethanol production facilities. For example, instead of transporting biomass to the production facility, the facility is transported to the biomass or is located nearby the source of the biomass. The ethanol production facilities or components thereof may be transported via land, water, or air. Production of other products, such as hydrocarbons, natural gas, hydrogen gas, plastics, polymers, and proteins, can also be made by the methods and facilities. Any product described herein can be made in finished form or un-finished form and moved, e.g., to a fixed facility, e.g.Type: ApplicationFiled: February 5, 2013Publication date: June 13, 2013Applicant: XYLECO, INC.Inventor: XYLECO, INC.
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Publication number: 20130149757Abstract: A novel Clostridium has been found that produces primarily n-butanol and isopropanol. Increased butanol was obtained by growing it continuously in an immobilized structure and extracting fermentation products immediately thereafter in a continuous flow extraction medium. Increased production was also achieved by fermentation in the presence of an extraction medium (such as corn oil) to decrease product inhibition followed by product separation from the fermentation broth and the extraction medium.Type: ApplicationFiled: December 7, 2012Publication date: June 13, 2013Applicant: OPTINOL, INC.Inventor: Optinol, Inc.
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Patent number: 8460898Abstract: Biomass is pretreated using an organic solvent solution under alkaline conditions in the presence of ammonia and optionally an additional nucleophile to fragment and extract lignin without loss of hemicellulose. Pretreated biomass is further hydrolyzed with a saccharification enzyme consortium. Fermentable sugars released by saccharification may be utilized for the production of target chemicals by fermentation.Type: GrantFiled: July 2, 2012Date of Patent: June 11, 2013Assignee: E I du Pont de Nemours and CompanyInventors: Bruce A Diner, Janine Fan
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Patent number: 8460901Abstract: The instant invention pertains to the use of formic acid in new processes for treating feedstocks comprising holocellulose, as well as, new compositions suitable for, for example, bioalcohol production. The processes may comprise first mixing the feedstock with an aqueous acid solution comprising formic acid and then producing a hydrolysis product comprising monosaccharides and water-soluble oligosaccharides. The compositions typically comprise a feedstock comprising holocellulose and an aqueous acid solution comprising formic acid. Advantageously, the processes and compositions of the present invention may be used in more environmentally friendly, cost-efficient production of fuels.Type: GrantFiled: May 18, 2010Date of Patent: June 11, 2013Assignee: Georgia Tech Research CorporationInventors: Teresita Marzialetti, Christopher W. Jones, Pradeep Agrawal
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Patent number: 8460906Abstract: Methods for mutagenizing and selecting microorganisms with increased product tolerance are provided. Additionally, methods and systems are disclosed for culturing the microorganisms to produce products.Type: GrantFiled: April 9, 2009Date of Patent: June 11, 2013Assignee: Cobalt Technologies, Inc.Inventors: Pamela Reilly Contag, Stacy M. Burns-Guydish, Hendrikus J. Meerman, David C. Walther, John C. Chen, Alvin W. Nienow, Ian Maddox
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Publication number: 20130143288Abstract: Isolated bacteria are disclosed. The isolated bacteria are aerotolerant and can produced butanol. Methods are disclosed to produce generated chemicals. Methods are disclosed to isolate aerotolerant bacteria.Type: ApplicationFiled: August 11, 2011Publication date: June 6, 2013Applicant: The Administrators of the Tulane Educational FundInventors: David A. Mullin, Harshad R. Velankar
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Publication number: 20130143277Abstract: The present disclosure is directed, in a first aspect, to the use of inverting beta-xylosidase enzymes to reduce byproduct formation and increase the yield of fermentation products, as well as, in a second aspect, to the use of retaining beta-xylosidase enzymes to improve production of alkyl-beta-xylopyranoside compounds, in a simultaneous saccharification and fermentation reactions.Type: ApplicationFiled: December 17, 2010Publication date: June 6, 2013Applicant: DANISCO US INC.Inventors: Christina Gutierrez, Colin Mitchinson, Tom T. Huang, Bruce A. Diner, Paul Joseph Fagan, William D. Hitz
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Publication number: 20130143289Abstract: A method of pre-treating a cellulosic material before hydrolysis is provided. The method comprises the steps of: impregnating the cellulosic material with a reactive water-soluble gas, such as sulphur dioxide (SO2) or carbon dioxide (CO2), in an impregnation chamber to obtain impregnated material; and heating the impregnated material to obtain pre-treated material, wherein the cellulosic material is compressed right before or when it is transferred to the impregnation chamber. A corresponding system is also provided.Type: ApplicationFiled: December 17, 2010Publication date: June 6, 2013Inventors: Torbjörn Van Der Meulen, Stanley Forss, Lars Elfving, Staffan Magnusson, Magnus Hägglund, Anders Sjöblom
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Patent number: 8455224Abstract: A high flux of metabolites from pyruvate to 2,3-butanediol in Lactobacillus plantarum was achieved through genetic engineering. Substantial elimination of lactate dehydrogenase activity in the presence of heterologously expressed butanediol dehydrogenase activity led to 2,3 butanediol production that was at least 49% of the total of major pyruvate-derived products.Type: GrantFiled: September 29, 2009Date of Patent: June 4, 2013Assignee: Butamax(TM) Advanced Biofuels LLCInventor: Brian James Paul
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Patent number: 8455225Abstract: Increasing tolerance to butanol in yeast has been accomplished by increasing activity of the high osmolarity/glycerol response pathway. Yeast with increased expression of PBS2p, a mitogen activated protein kinase kinase of the MAPK module of the high osmolarity/glycerol response pathway may be used for improved butanol production.Type: GrantFiled: December 21, 2009Date of Patent: June 4, 2013Assignee: Butamax Advanced Biofuels LLCInventors: Michael G. Bramucci, Robert A. Larossa, Dana R. Smulski
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Patent number: 8455239Abstract: The present invention provides recombinant mircoorganisms comprising an isobutanol producing metabolic pathway and methods of using said recombinant microorganisms to produce isobutanol. In various aspects of the invention, the recombinant microorganisms may comprise a modification resulting in the reduction of pyruvate decarboxylase and/or glycerol-3-phosphate dehydrogenase activity. In various embodiments described herein, the recombinant microorganisms may be microorganisms of the Saccharomyces clade, Crabtree-negative yeast microorganisms, Crabtree-positive yeast microorganisms, post-WGD (whole genome duplication) yeast microorganisms, pre-WGD (whole genome duplication) yeast microorganisms, and non-fermenting yeast microorganisms.Type: GrantFiled: June 22, 2010Date of Patent: June 4, 2013Assignee: Gevo, Inc.Inventors: Reid M. Renny Feldman, Uvini Gunawardena, Jun Urano, Peter Meinhold, Aristos Aristidou, Catherine Asleson Dundon, Christopher Smith
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Patent number: 8450093Abstract: Adaptive engineering of microorganisms to create mutants exhibiting high butanol tolerance and productivity. Mutant strains are obtained through the fermentation of parental strains in a fibrous bed bioreactor in the presence of butanol. Also provided are methods of producing butanol using mutant strains in ABE fermentation. Butanol production may be furthered by gas stripping and distillation. Also provided are mutant proteins for increasing butanol production in a microorganism.Type: GrantFiled: November 9, 2011Date of Patent: May 28, 2013Assignee: The Ohio State UniversityInventors: Shang-Tian Yang, Jingbo Zhao
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Publication number: 20130130341Abstract: The disclosure provides a process that converts CO2 to higher alcohols (e.g. isobutanol) using electricity as the energy source. This process stores electricity (e.g. from solar energy, nuclear energy, and the like) in liquid fuels that can be used as high octane number gasoline substitutes. Instead of deriving reducing power from photosynthesis, this process derives reducing power from electrically generated mediators, either H2 or formate. H2 can be derived from electrolysis of water. Formate can be generated by electrochemical reduction of CO2. After delivering the reducing power in the cell, formate becomes CO2 and recycles back. Therefore, the biological CO2 fixation process can occur in the dark.Type: ApplicationFiled: January 15, 2011Publication date: May 23, 2013Applicant: THE REGENTS OF THE UNIVERSITY OF CALIFORNIAInventors: James C. Liao, Kwang Myung Cho
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Publication number: 20130130335Abstract: Methods of producing renewable materials may include consuming a fermentation feedstock with a fermentation organism to produce a renewable material in fermentation broth; water may then be separated from the feedstock or broth using one or more phase separations, or the renewable material may be concentrated from the feedstock or broth using one or more phase separations. Methods of producing biofuel components may include consuming a lignocellulosic or sugar fermentation feedstock with a fermentation organism to produce either ethanol or butanol in fermentation broth; cooling the feedstock or broth to solidify at least some water therein; and separating the solidified water from the feedstock or broth using a solid-liquid phase separation.Type: ApplicationFiled: November 21, 2012Publication date: May 23, 2013Applicant: BP Corporation North America Inc.Inventors: Glen Austin, Binita X. Bhattacharjee, Leslie W. Bolton, Jacob Borden, Martin E. Carrera, Amit A. Gokhale, Chris Horler, Aidan Hurley, Eric T. Mack
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Publication number: 20130130342Abstract: Yeast cells with a reduced general control response to amino acid starvation were found to have increased tolerance to butanol in the growth medium. The reduced response was engineered by genetic modification of a gene involved in the response, a GCN gene, to eliminate activity of the encoded protein. Yeast strains with an engineered butanol biosynthetic pathway and a genetic modification in a gene involved in the general control response to amino acid starvation, which have increased butanol tolerance, are useful for production of butanol.Type: ApplicationFiled: February 1, 2013Publication date: May 23, 2013Applicant: Butamax(TM) Advanced Biofuels LLCInventor: Butamax(TM) Advanced Biofuels LLC
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Publication number: 20130130318Abstract: A method is presented for the production of cellulosic ethanol, acetic acid and derivatives from the extract containing fibers and hemicelluloses after steam cooking of biomass in a host plant. The process is integrated with the host plant process to minimize the effect of loss of heat value from the extracted hemicelluloses and eliminate the need for the waste water treatment plant.Type: ApplicationFiled: May 15, 2012Publication date: May 23, 2013Applicant: API INTELLECTUAL PROPERTY HOLDINGS, LLCInventors: Theodora RETSINA, Vesa PYLKKANEN, Kimberly NELSON