Acyclic Patents (Class 435/157)
  • Patent number: 11028413
    Abstract: The invention relates to the production of one or more terpenoids through microbial engineering, and relates to the manufacture of products comprising terpenoids.
    Type: Grant
    Filed: March 5, 2020
    Date of Patent: June 8, 2021
    Assignees: Massachusetts Institute of Technology, National University of Singapore
    Inventors: Parayil K. Ajikumar, Gregory Stephanopoulos, Heng Phon Too
  • Patent number: 10900060
    Abstract: The present invention relates to a recombinant Deinococcus bacterium exhibiting enhanced 2-C-methyl-D-erythritol 4-phosphate/1-deoxy-D-xylulose 5-phosphate (MEP/DXP) pathway, and its use for producing terpene or terpenoid compounds.
    Type: Grant
    Filed: June 15, 2015
    Date of Patent: January 26, 2021
    Assignee: DEINOVE
    Inventors: Nicolas Chabot, Sandra Castang, Philippe Bauchart, Jean-Paul Leonetti
  • Patent number: 10844409
    Abstract: A method of producing lipids, containing the steps of: culturing a transformant wherein the expressions of a gene encoding any one of the following proteins (A) to (F) and a gene encoding an acyl-ACP thioesterase are enhanced, and producing fatty acids or lipids containing these fatty acids as components: (A) a protein consisting of the amino acid sequence set forth in SEQ ID NO: 2; (B) a protein consisting of an amino acid sequence having 89% or more identity with the amino acid sequence of the protein (A), and having acyl-CoA synthetase activity; (C) a protein consisting of the amino acid sequence set forth in SEQ ID NO: 4; (D) a protein consisting of an amino acid sequence having 49% or more identity with the amino acid sequence of the protein (C), and having acyl-CoA synthetase activity; (E) a protein consisting of the amino acid sequence set forth in SEQ ID NO: 6; and (F) a protein consisting of an amino acid sequence having 85% or more identity with the amino acid sequence of the protein (E), a
    Type: Grant
    Filed: March 31, 2017
    Date of Patent: November 24, 2020
    Assignee: Kao Corporation
    Inventor: Shinji Sugihara
  • Patent number: 10648006
    Abstract: This invention relates to modified hydroxylases. The invention further relates to cells expressing such modified hydroxylases and methods of producing hydroxylated alkanes by contacting a suitable substrate with such cells.
    Type: Grant
    Filed: March 30, 2018
    Date of Patent: May 12, 2020
    Assignee: California Institute of Technology
    Inventors: Frances Arnold, Peter Meinhold, Matthew W. Peters, Rudi Fasan, Mike M. Y. Chen
  • Patent number: 10584310
    Abstract: An integrated system to produce microalgae with autonomous fault identification and circumvention that can be deployed on land or at sea. The system comprises a fully scalable reactor, CO2 extraction, oxygen replenishment to surrounding water, and all necessary equipment to run it safely ensuring high energy efficiency and optimal control of environment variables to maximize biomass yield.
    Type: Grant
    Filed: March 8, 2017
    Date of Patent: March 10, 2020
    Inventor: Ivan Araujo Dayrell
  • Patent number: 10563229
    Abstract: The present disclosure relates to the use of a switch for the production of heterologous non-catabolic compounds in microbial host cells. In one aspect, provided herein are genetically modified microorganisms that produce non-catabolic compounds more stably when serially cultured under aerobic conditions followed by microaerobic conditions, and methods of producing non-catabolic compounds by culturing the genetically modified microbes under such culture conditions. In another aspect, provided herein are genetically modified microorganisms that produce non-catabolic compounds more stably when serially cultured in the presence of maltose followed by the reduction or absence of maltose, and methods of producing non-catabolic compounds by culturing the genetically modified microbes under such culture conditions.
    Type: Grant
    Filed: August 7, 2013
    Date of Patent: February 18, 2020
    Assignees: Amyris, Inc., Total Marketing Services
    Inventors: Penelope R. Chua, Adam Meadows
  • Patent number: 10519470
    Abstract: The invention relates to the production of one or more terpenoids through microbial engineering, and relates to the manufacture of products comprising terpenoids.
    Type: Grant
    Filed: March 19, 2018
    Date of Patent: December 31, 2019
    Assignees: Massachusetts Institute of Technology, National University of Singapore
    Inventors: Parayil K. Ajikumar, Gregory Stephanopoulos, Heng Phon Too
  • Patent number: 10435721
    Abstract: Provided herein are Metschnikowia species that produce xylitol from xylose when cultured, as well as methods to make and use these Metschnikowia species.
    Type: Grant
    Filed: December 20, 2017
    Date of Patent: October 8, 2019
    Assignee: Creatus Biosciences Inc.
    Inventors: Zongli Luo, Hendrik Jurgens Jansen van Vuuren, Allan George DeBono, Andrew Taplin Ferguson
  • Patent number: 10400254
    Abstract: The present invention relates to terpene synthases capable of degrading precursors into biofuel compounds, such as terpenoid compounds. In one instance, a transformed organism can include such terpene synthases, as well as vectors encoding such synthases. Methods of employing such synthases and organisms are also described herein.
    Type: Grant
    Filed: March 10, 2016
    Date of Patent: September 3, 2019
    Assignee: National Technology & Engineering Solutions of Sandia, LLC
    Inventors: Weihua Wu, John Michael Gladden, Benjamin Chiau-Pin Wu, Ryan Wesley Davis
  • Patent number: 10202620
    Abstract: In certain aspects, the present invention provides methods for producing terminally hydroxylated alkenes and alkynes by contacting an unsaturated or saturated hydrocarbon substrate with a hydroxylase enzyme. Exemplary terminal hydroxylases useful for carrying out the methods of the invention exhibit strong selectivity towards one terminal carbon of a hydrocarbon substrate and include, but are not limited to, non-heme diiron alkane monooxygenases, cytochromes P450 (e.g., cytochromes P450 of the CYP52 and CYP153 family), as well as long chain alkane hydroxylases. In some embodiments, the terminally hydroxylated alkene or alkyne is further converted to a terminal alkenal. In certain embodiments, terminally hydroxylated alkenes and alkynes are useful as insect pheromones which modify insect behavior. In other embodiments, terminally hydroxylated alkenes and alkynes are useful intermediates for producing pheromones via acetylation or oxidation of the alcohol moiety.
    Type: Grant
    Filed: September 3, 2015
    Date of Patent: February 12, 2019
    Assignee: PROVIVI, INC.
    Inventors: Pedro Coelho, Mike M. Y. Chen, Peter Meinhold, Catherine Mee-Hie Cho, Vu Bui, Thomas Heel
  • Patent number: 9862976
    Abstract: Methods for the fermentative production of four carbon alcohols is provided. Specifically, butanol, preferably isobutanol is produced by the fermentative growth of a recombinant bacterium expressing an isobutanol biosynthetic pathway.
    Type: Grant
    Filed: March 11, 2016
    Date of Patent: January 9, 2018
    Assignee: Butamax Advanced Biofuels LLC
    Inventors: Gail K. Donaldson, Andrew C. Eliot, Dennis Flint, Lori Ann Maggio-Hall, Vasantha Nagarajan
  • Patent number: 9803209
    Abstract: Provided herein are Lactobacillus mutants having improved transformation efficiency, comprising a disruption to an endogenous gene encoding a restriction modification system protein. Also described are methods for producing the mutants, methods for generating transformants using the mutants, and methods for producing a polypeptide or fermentation product using the mutants.
    Type: Grant
    Filed: May 17, 2013
    Date of Patent: October 31, 2017
    Assignee: Novozymes A/S
    Inventors: Steen Troels Joergensen, Torsten Bak Regueira, Brian Kobmann, Peter Bjarke Olsen, Bjarke Christensen
  • Patent number: 9752163
    Abstract: Described is a method for the enzymatic production of isoprenol using mevalonate as a substrate and enzymatically converting it by a decarboxylation step into isoprenol as well as the use of an enzyme which is capable of catalyzing the decarboxylation of mevalonate for the production of isoprenol from mevalonate. Furthermore described is the use of mevalonate as a starting material for the production of isoprenol in an enzymatically catalyzed reaction.
    Type: Grant
    Filed: July 7, 2015
    Date of Patent: September 5, 2017
    Assignees: Global Bioenergies, Scientist of Fortune SA
    Inventors: Philippe Marliere, Maria Anissimova, Romain Chayot, Marc Delcourt
  • Patent number: 9506071
    Abstract: Methods for the fermentative production of four carbon alcohols is provided. Specifically, butanol, preferably isobutanol is produced by the fermentative growth of a recombinant bacterium expressing an isobutanol biosynthetic pathway.
    Type: Grant
    Filed: May 19, 2015
    Date of Patent: November 29, 2016
    Assignee: Butamax Advanced Biofuels LLC
    Inventors: Gail K. Donaldson, Andrew C. Eliot, Dennis Flint, Lori Ann Maggio-Hall, Vasantha Nagarajan
  • Patent number: 9476082
    Abstract: Yeast strains capable of increased prenyl phosphate production are provided, enabling increased terpenoid molecule production. Heterologous yeast strains with high prenyl phosphate availability are prepared using one or both of two different strategies for increasing the availability of prenyl phosphates for terpenoid production. First, by co-expressing multiple mevalonate pathway gene analogs, a novel heterologous combination of genes results, some of which increases the inherent availability of prenyl phosphates in yeast. Second, by expressing the non-endogenous enzyme ATP citrate lysase (ACL), a buildup of high cytosolic concentration of acetyl-CoA is produced in the cytosol of S. cerevisiae.
    Type: Grant
    Filed: May 20, 2011
    Date of Patent: October 25, 2016
    Assignee: Evolva SA
    Inventor: Jorgen Hansen
  • Patent number: 9441250
    Abstract: Provided herein are glycerol-3-phosphate dehydrogenase (GPD) enzymes with increased KM for NADH and GPD enzymes with substantially the same affinity for NADH and NADPH and/or are feedback inhibited by glycerol-3-phosphate. Also provided herein are recombinant microorganisms comprising a heterologous gene encoding GPD and a deletion or disruption in an endogenous gene encoding GPD. Also provided are recombinant microorganisms comprising a heterologous gene encoding GPD and a butanol biosynthetic pathway. Further provided are methods of producing butanol comprising providing the recombinant microorganisms described herein and contacting the recombinant microorganism with at least one fermentable carbon substrate under conditions wherein butanol is produced.
    Type: Grant
    Filed: March 13, 2014
    Date of Patent: September 13, 2016
    Assignee: Butamax Advanced Biofuels LLC
    Inventors: Ritu Bhalla, Gopal K Chotani, Michael Dauner, Mark J Nelson, Daniel P O'Keefe, Caroline M Peres, Jahnavi Chandra Prasad, Jean-Francois Tomb
  • Patent number: 9404117
    Abstract: Yeast cells with reduced activity of certain enzymes involved in branched chain amino acid biosynthesis in yeast mitochondria are described. Target enzymes include threonine deaminase, isopropylmalate synthase, and optionally branched chain amino acid transaminase.
    Type: Grant
    Filed: September 9, 2015
    Date of Patent: August 2, 2016
    Assignee: Butamax Advanced Biofuels LLC
    Inventor: Larry Cameron Anthony
  • Patent number: 9404096
    Abstract: This invention relates to modified hydroxylases. The invention further relates to cells expressing such modified hydroxylases and methods of producing hydroxylated alkanes by contacting a suitable substrate with such cells.
    Type: Grant
    Filed: June 30, 2015
    Date of Patent: August 2, 2016
    Assignee: California Institute of Technology
    Inventors: Frances Arnold, Peter Meinhold, Matthew W. Peters, Rudi Fasan, Mike M. Y. Chen
  • Patent number: 9365872
    Abstract: Methods for the fermentative production of four carbon alcohols is provided. Specifically, butanol, preferably isobutanol is produced by the fermentative growth of a recombinant bacterium expressing an isobutanol biosynthetic pathway.
    Type: Grant
    Filed: May 19, 2015
    Date of Patent: June 14, 2016
    Assignee: Butamax Advanced Biofuels LLC
    Inventors: Gail K. Donaldson, Andrew C. Eliot, Dennis Flint, Lori Ann Maggio-Hall, Vasantha Nagarajan
  • Patent number: 9322072
    Abstract: The present invention generally provides methods of improving lignin separation during biomass fractionation with an acid to release sugars and a solvent for lignin (such as ethanol). In some embodiments, a digestor is employed to fractionating a feedstock in the presence of a solvent for lignin, sulfur dioxide, and water, to produce a liquor containing hemicellulose, cellulose-rich solids, and lignin. A solid additive is added to the digestor, wherein the solid additive combines with at least a portion of the lignin. Then a mixture of lignin and the solid additive is separated from the liquor, prior to hemicellulose recovery. Optionally, a solid additive may also be introduced to a hydrolysis reactor for converting hemicellulose oligomers to monomers, to improve separation of acid-catalyzed lignin. In some embodiments, the solid additive is gypsum or a gypsum/lignin mixture.
    Type: Grant
    Filed: October 29, 2013
    Date of Patent: April 26, 2016
    Assignee: API Intellectual Property Holdings, LLC
    Inventors: Theodora Retsina, Vesa Pylkkanen, Kimberly Nelson, Mark Szczepanik
  • Patent number: 9297029
    Abstract: Methods for the fermentative production of four carbon alcohols is provided. Specifically, butanol, preferably isobutanol is produced by the fermentative growth of a recombinant bacterium expressing an isobutanol biosynthetic pathway.
    Type: Grant
    Filed: December 30, 2014
    Date of Patent: March 29, 2016
    Assignee: Butamax Advanced Biofuels LLC
    Inventors: Gail K. Donaldson, Andrew C. Eliot, Dennis Flint, Lori Ann Maggio-Hall, Vasantha Nagarajan
  • Patent number: 9297016
    Abstract: The present invention is related to a recombinant host cell, in particular a yeast cell, comprising a dihydroxy-acid dehydratase polypeptide. The invention is also related to a recombinant host cell having increased specific activity of the dihydroxy-acid dehydratase polypeptide as a result of increased expression of the polypeptide, modulation of the Fe—S cluster biosynthesis of the cell, or a combination thereof. The present invention also includes methods of using the host cells, as well as, methods for identifying polypeptides that increase the flux in an Fe—S cluster biosynthesis pathway in a host cell.
    Type: Grant
    Filed: February 17, 2011
    Date of Patent: March 29, 2016
    Assignee: Butamax Advanced Biofuels LLC
    Inventors: Dennis Flint, Brian James Paul, Rick W. Ye
  • Patent number: 9279136
    Abstract: Methods and compositions for the production of food compositions, oils, fuels, oleochemicals, and other compounds in recombinant microorganisms are provided, including oil-bearing microorganisms and methods of low cost cultivation of such microorganisms. Microalgal cells containing exogenous genes encoding, for example, a lipase, a sucrose transporter, a sucrose invertase, a fructokinase, a polysaccharide-degrading enzyme, a keto acyl-ACP synthase enzyme, a fatty acyl-ACP thioesterase, a fatty acyl-CoA/aldehyde reductase, a fatty acyl-CoA reductase, a fatty aldehyde reductase, a fatty aldehyde decarbonylase, and/or an acyl carrier protein are useful in manufacturing food compositions, and transportation fuels such as renewable diesel, biodiesel, and renewable jet fuel, as well as oleochemicals such as functional fluids, surfactants, soaps and lubricants.
    Type: Grant
    Filed: September 28, 2012
    Date of Patent: March 8, 2016
    Assignee: Solazyme, Inc.
    Inventors: Scott Franklin, Aravind Somanchi, Janice Wee, George Rudenko, Jeffrey L. Moseley, Walt Rakitsky
  • Patent number: 9267157
    Abstract: The invention relates to recombinant host cells having at least one integrated polynucleotide encoding a polypeptide that catalyzes a step in a pyruvate-utilizing biosynthetic pathway, e.g., pyruvate to acetolactate conversion. The invention also relates to methods of increasing the biosynthetic production of isobutanol, 2,3-butanediol, 2-butanol or 2-butanone using such host cells.
    Type: Grant
    Filed: September 7, 2011
    Date of Patent: February 23, 2016
    Assignee: Butamax Advanced Biofuels LLC
    Inventors: Larry Cameron Anthony, Lori Ann Maggio-Hall
  • Patent number: 9267156
    Abstract: An isopropyl alcohol-producing Escherichia coli includes an isopropyl alcohol production system, wherein an activity of transcriptional repressor GntR is inactivated, and the isopropyl alcohol-producing Escherichia coli preferably further includes a group of auxiliary enzymes having an enzyme activity expression pattern with which isopropyl alcohol production capacity achieved by the inactivation of the GntR activity is maintained or enhanced. A method of producing isopropyl alcohol includes producing isopropyl alcohol from a plant-derived raw material using the isopropyl alcohol-producing Escherichia coli. A method of producing acetone includes contacting the isopropyl alcohol obtained by the isopropyl alcohol production method with a complex oxide that includes zinc oxide and at least one oxide containing a Group 4 element, and that is prepared by coprecipitation.
    Type: Grant
    Filed: August 11, 2011
    Date of Patent: February 23, 2016
    Assignee: MITSUI CHEMICALS, INC.
    Inventors: Koh Amano, Tomokazu Shirai, Hitoshi Takahashi, Junichiro Hirano, Yoshiko Matsumoto, Nozomi Takebayashi, Mitsufumi Wada, Hiroshi Shimizu, Chikara Furusawa, Takashi Hirasawa
  • Patent number: 9249076
    Abstract: Provided herein are processes comprising providing a fermentation medium comprising a fermentable carbon source, a recombinant microorganism comprising an engineered butanol biosynthetic pathway, and butanol; contacting said fermentation medium with an extractant composition comprising an effective amount of antioxidant or antioxidant-like compound, whereby at least a portion of the butanol in the fermentation medium partitions into the extractant; recovering at least a portion of the butanol and extractant composition from the fermentation medium; recycling the extractant composition recovered in (c) one or more times in the fermentation medium; optionally adjusting the effective amount of antioxidant or antioxidant-like compound in the extractant composition, whereby the rate of oxidation or effect of oxidized products in the recycled extractant composition is substantially reduced and/or avoided such that the extractant composition may be recycled.
    Type: Grant
    Filed: September 20, 2013
    Date of Patent: February 2, 2016
    Assignee: BUTAMAX ADVANCED BIOFUELS LLC
    Inventors: Douglas Robert Anton, Bruce A. Diner, Steven D. Doig
  • Patent number: 9234203
    Abstract: One aspect of the present disclosure relates to a stabilized recombinant expression plasmid vector comprising a polynucleotide encoding an antitoxin gene which expresses a polypeptide that neutralizes a polypeptide toxic to a host cell, the toxic polypeptide being expressed by a toxin gene in the host cell, and a polynucleotide encoding a polypeptide expression product, and the stabilized recombinant expression plasmid vector is derived from a Hafnia alvei autonomously replicable backbone plasmid. Other aspects of the present disclosure relate to a transformant transformed with the stabilized recombinant expression plasmid vector disclosed herein, a method of producing biobased cadaverine using the transformant disclosed herein, and biobased cadaverine prepared by the method disclosed herein. Another aspect of the present disclosure relates to a polyamide formed using biobased cadaverine disclosed herein, and a composition thereof.
    Type: Grant
    Filed: March 12, 2013
    Date of Patent: January 12, 2016
    Assignees: Cathay Industrial Biotech Ltd., Cathay R&D Center Co., Ltd.
    Inventors: Zhenhua Pang, Naiqiang Li, Charlie Liu, Xiucai Liu
  • Patent number: 9228214
    Abstract: In a process for the enantioselective enzymatic reduction of a hydroxy ketone of general formula I wherein R1?C1-C6 alkyl and R2?—Cl, —CN, —OH, —H or C1-C6 alkyl, into a chiral diol of general formula II wherein R1 and R2 have the same meaning as in formula I, the hydroxy ketone is reduced with an oxidoreductase in the presence of NADH or NADPH as a cofactor, wherein a) the hydroxy ketone is provided in the reaction at a concentration of ?50 g/l, b) the oxidized cofactor NAD or NADP having formed is regenerated continuously by oxidation of a secondary alcohol of general formula RXRYCHOH, wherein RX, RY independently represent hydrogen, branched or unbranched C1-C8-alkyl and Ctotal?3, and c) the reduction of the hydroxy ketone and the oxidation of the secondary alcohol are catalyzed by the same oxidoreductase.
    Type: Grant
    Filed: February 4, 2015
    Date of Patent: January 5, 2016
    Assignee: CAMBREX IEP GMBH
    Inventors: Antje Gupta, Maria Dupont, Anke Tschentscher
  • Patent number: 9217183
    Abstract: Isopropanol is produced with good productivity via fermentation processes. Specifically, isopropanol is produced at a high level in a medium by culturing recombinant yeast into which an acetoacetyl CoA synthase gene and a group of genes (isopropanol synthesis-related gene group) encoding a group of enzymes for synthesis of isopropanol from acetoacetyl CoA have been introduced.
    Type: Grant
    Filed: July 29, 2014
    Date of Patent: December 22, 2015
    Assignee: TOYOTA JIDOSHA KABUSHIKI KAISHA
    Inventors: Masayoshi Muramatsu, Satoshi Yoneda
  • Patent number: 9163221
    Abstract: The present disclosure provides methods useful for producing fatty alcohol compositions from recombinant host cells. The disclosure further provides variant fatty acyl-CoA reductase (FAR) enzymes, polynucleotides encoding the variant FAR enzymes, and vectors and host cells comprising the same.
    Type: Grant
    Filed: September 27, 2013
    Date of Patent: October 20, 2015
    Assignee: CODEXIS, INC.
    Inventors: Behnaz Behrouzian, Louis Clark, Yihui Zhu, Michael Clay, Kristian Karlshoej
  • Patent number: 9145542
    Abstract: The invention provides recombinant microorganisms capable of producing isobutyraldehyde using CO2 as a carbon source. The invention further provides methods of preparing and using such microorganisms to produce isobutyraldehyde.
    Type: Grant
    Filed: June 20, 2011
    Date of Patent: September 29, 2015
    Assignee: The Regents of the University of California
    Inventors: James C. Liao, Shota Atsumi
  • Patent number: 9096842
    Abstract: The present invention provides an isopropyl alcohol-producing Escherichia coli that includes at least a sucrose hydrolase gene that belongs to a sucrose non-PTS gene group, and an imparted or enhanced isopropyl alcohol production system, and an isopropyl alcohol production method of producing isopropyl alcohol from a sucrose-containing plant-derived raw material using the isopropyl alcohol-producing Escherichia coli.
    Type: Grant
    Filed: September 13, 2010
    Date of Patent: August 4, 2015
    Assignee: MITSUI CHEMICALS, INC.
    Inventors: Takashi Morishige, Hitoshi Takahashi, Nozomi Takebayashi, Mitsufumi Wada
  • Patent number: 9085780
    Abstract: Disclosed is a method for producing (S)-1,1,1-trifluoro-2-propanol with high optical purity and high yield by having at least one kind of microorganism, which is selected from the group consisting of Hansenula polymorpha, Pichia anomala, Candida parapsilosis, Candida mycoderma, Pichia naganishii, Candida saitoana, Cryptococcus curvatus, Saturnospora dispora, Saccharomyces bayanus and Pichia membranaefaciens, act on 1,1,1-trifluoroacetone. Since microorganisms found in nature are made to act in a natural state, the problems to be raised when a transformant or the like is used can be avoided in this method. Consequently, the method can be easily put in industrial practice.
    Type: Grant
    Filed: February 14, 2011
    Date of Patent: July 21, 2015
    Assignees: Toyama Prefecture, Central Glass Company, Limited
    Inventors: Yasuhisa Asano, Kenichi Fuhshuku, Tetsuro Nishii, Akihiro Ishii
  • Patent number: 9040266
    Abstract: Disclosed is a process in which a recombinant organism, such as a yeast, expressing a heterologous S-adenosylmethionine (SAM)-dependent methyl halide transferase (MHT) protein is combined with a halide and a carbon source in a cultivation medium under conditions in which methyl formate is produced. The cell may genetically modified to express methyl formate synthase, methanol dehydrogenase and/or hydrolytic dehalogenase at levels higher than a cell of the same species that is not genetically modified. The methyl formate may be collected and used in a variety of applications. The halide may be chlorine, bromine or iodine.
    Type: Grant
    Filed: July 22, 2010
    Date of Patent: May 26, 2015
    Assignee: The Regents of the University of California
    Inventors: Christopher A. Voigt, Travis S. Bayer
  • Patent number: 9034617
    Abstract: Processes are disclosed for the anaerobic bioconversion of syngas to oxygenated organic compound that use an in situ method for protecting the microorganisms from hydrogen cyanide contained in the syngas that passes to the fermentation broth. The fermentation broth is maintained at a pH of between about 4 and 6, and dissolved metal cation of one or more of iron, cobalt, nickel and zinc is provided to the fermentation broth in an amount sufficient to form, under the conditions of the fermentation broth, a substantially insoluble metal complex with the metal cation and cyanide anion. The rate of formation of the insoluble complex is sufficiently high that that the amount of cyanide that is taken up by microorganisms does not result in an undue adverse effect on the population of microorganisms.
    Type: Grant
    Filed: March 14, 2013
    Date of Patent: May 19, 2015
    Assignee: Coskata, Inc.
    Inventors: Robert Hickey, Jianxin Du, Andrew Reeves, Richard E. Tobey
  • Publication number: 20150133698
    Abstract: The invention generally relates to the production of a fatty alcohol composition from recombinant microbial cells. The fatty alcohols are produced by expressing a gene encoding a heterologous fatty alcohol forming acyl-CoA reductase (“FAR”); a gene encoding a heterologous thioesterase (“TE”) gene and a gene encoding an acyl-CoA synthetase (“ACS”).
    Type: Application
    Filed: April 19, 2013
    Publication date: May 14, 2015
    Inventors: Kaman Chan, Fernando Valle, Yoram Barak, Louis Clark, Kristian Karlshoej
  • Publication number: 20150132816
    Abstract: The invention relates to recombinant cells and their use in the production of branched medium-chain alcohols such as 4-methyl-1-pentanol.
    Type: Application
    Filed: October 31, 2014
    Publication date: May 14, 2015
    Inventors: Kristala Lanett Jones Prather, Micah James Sheppard, Aditya Kunjapur
  • Publication number: 20150132815
    Abstract: Processes are disclosed for the low energy, anaerobic bioconversion of hydrogen and carbon monoxide in a gaseous substrate stream to oxygenated organic compounds such as ethanol by contact with microorganisms in a deep, tank fermentation system with high conversion efficiency of both hydrogen and carbon monoxide. Gas feed to the reactor is injected using a motive liquid to form a stable dispersion of microbubbles thereby reducing energy costs, and a portion of the off-gases from the reactor are recycled to (i) achieve a conversion of the total moles of carbon monoxide and hydrogen in the gas substrate to oxygenated organic compound of at least about 80 percent and (ii) attenuate the risk of carbon monoxide inhibition of the microorganism used for the bioconversion.
    Type: Application
    Filed: January 19, 2015
    Publication date: May 14, 2015
    Applicant: Coskata, Inc.
    Inventor: Robert Hickey
  • Patent number: 9029124
    Abstract: The present disclosure identifies methods and compositions for modifying photoautotrophic organisms as hosts, such that the organisms efficiently convert inorganic carbon and light into n-alkanes, and in particular the use of such organisms for the commercial production of n-alkanes and related molecules.
    Type: Grant
    Filed: March 23, 2012
    Date of Patent: May 12, 2015
    Assignee: Joule Unlimited Technologies, Inc.
    Inventors: Dan Eric Robertson, Christian Perry Ridley, Nikos Basil Reppas, Jacob Carter Harrison, Michael Connor, Frank Anthony Skraly
  • Publication number: 20150125906
    Abstract: The present invention relates to methods of degrading or converting biomass material enriched with hemicellulosic material into fermentable sugars.
    Type: Application
    Filed: January 14, 2015
    Publication date: May 7, 2015
    Inventors: Prashant Iyer, Harry Showmaker, Hui Xu, Kishore Rane
  • Publication number: 20150125902
    Abstract: Provided are isolated polypeptides having glucoamylase activity, catalytic domains, and polynucleotides encoding the polypeptides, catalytic domains. Also provided are nucleic acid constructs, vectors and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides, catalytic domains.
    Type: Application
    Filed: August 24, 2012
    Publication date: May 7, 2015
    Applicant: Novozymes A/S
    Inventors: Tianqi Sun, Ming Li
  • Publication number: 20150118725
    Abstract: Described is a method for generating isoprenol through a biological process. More specifically, described is a method for producing isoprenol from mevalonate.
    Type: Application
    Filed: April 4, 2013
    Publication date: April 30, 2015
    Inventors: Marc Delcourt, Maria Anissimova, Philippe Marliere
  • Patent number: 9012188
    Abstract: The present invention relates to producing chemicals and biofuels from wood material, e.g. mixed forest biomass. Specifically, the invention concerns a process for conditioning spent liquor produced by SO2-ethanol-water (SEW) fractionation of wood chips for fermentation to butanol, ethanol and acetone/isopropanol (so called ABE process) by Clostridia bacteria.
    Type: Grant
    Filed: March 16, 2012
    Date of Patent: April 21, 2015
    Assignee: API Intellectual Property Holdings, LLC
    Inventors: Adriaan Van Heiningen, Evangelos Sklavounos
  • Publication number: 20150093797
    Abstract: Provided herein are compositions and methods for the heterologous production of acetyl-CoA-derived isoprenoids in a host cell. In some embodiments, the host cell is genetically modified to comprise a heterologous nucleotide sequence encoding an acetaldehyde dehydrogenase, acetylating (ADA, E.C. 1.2.1.10) and an MEV pathway comprising an NADH-using HMG-CoA reductase. In some embodiments, the host cell is genetically modified to comprise a heterologous nucleotide sequence encoding an ADA and an MEV pathway comprising an acetoacetyl-CoA synthase. In some embodiments, the genetically modified host cell further comprises one or more heterologous nucleotide sequences encoding a phosphoketolase and a phosphotransacetylase. In some embodiments, the genetically modified host cell further comprises a functional disruption of the native PDH-bypass. The compositions and methods described herein provide an energy-efficient yet redox balanced route for the heterologous production of acetyl-CoA-derived isoprenoids.
    Type: Application
    Filed: September 2, 2014
    Publication date: April 2, 2015
    Applicant: AMYRIS, INC.
    Inventors: Timothy Stevens GARDNER, Kristy Michelle HAWKINS, Adam Leon MEADOWS, Annie Ening TSONG, Yoseph TSEGAYE
  • Publication number: 20150093790
    Abstract: The disclosure provides thermostable enzymes isolated from Caldicellulosiruptor bescii and fragments thereof useful for the degradation of cellulose and/or hemicellulose, including thermostable cellulases and hemicellulases. The disclosure further provides nucleic acids encoding the thermostable enzymes of the disclosure. The disclosure also provides methods for the conversion of cellulose and hemicellulose into fermentable sugars using thermostable enzymes of the disclosure. The disclosure also provides enzyme cocktails containing multiple enzymes disclosed herein. The enzymes can be used to release sugars present in cellulose or hemicellulose for subsequent fermentation to produce value-added products.
    Type: Application
    Filed: December 20, 2011
    Publication date: April 2, 2015
    Applicant: The Board pf Trustees of the University of Illinois
    Inventors: Yejun Han, Xiaoyun Su, Dylan Dodd, Roderick I. Mackie, Issac K.O. Cann
  • Patent number: 8993285
    Abstract: A non-naturally occurring microbial organism having an isopropanol pathway includes at least one exogenous nucleic acid encoding an isopropanol pathway enzyme. The pathway includes an enzyme selected from a 4-hydroxybutyryl-CoA dehydratase, a crotonase, a 3-hydroxybutyryl-CoA dehydrogenase, an acetoacetyl-CoA synthetase, an acetyl-CoA:acetoacetate-CoA transferase, an acetoacetyl-CoA hydrolase, an acetoacetate decarboxylase, and an acetone reductase. A non-naturally occurring microbial organism having an n-butanol pathway includes at least one exogenous nucleic acid encoding an n-butanol pathway enzyme. Other non-naturally occurring microbial organism have n-butanol or isobutanol pathways. The organisms are cultured to produce isopropanol, n-butanol, or isobutanol.
    Type: Grant
    Filed: April 30, 2010
    Date of Patent: March 31, 2015
    Assignee: Genomatica, Inc.
    Inventor: Anthony P. Burgard
  • Patent number: 8993284
    Abstract: An isolated nucleic acid molecule that encodes a terpene synthase and is selected from among: a) a nucleic acid molecule comprising the sequence of nucleotides set forth in SEQ ID NO: 1, SEQ ID NO: 3 or SEQ ID NO: 5; b) a nucleic acid molecule that is a fragment of (a); c) a nucleic acid molecule comprising a sequence of nucleotides that is complementary to (a) or (b); and d) a nucleic acid molecule that encodes a terpene synthase having at least or at least about 60%, 65%, 70%, 75%, 80%, 85%, 90%, 95%, 96%, 97%, 98%, 99% identity to any one of (a)-(c); wherein the nucleic acid molecule encodes a terpene synthase.
    Type: Grant
    Filed: September 24, 2013
    Date of Patent: March 31, 2015
    Assignees: The University of British Columbia, The University of Western Australia, Forest Products Commission
    Inventors: Katherine Zulak, Christopher Jones, Jessie Moniodis, Joerg Bohlmann
  • Publication number: 20150087028
    Abstract: The invention relates to recombinant expression of variant forms of M. thermophila CBH1a and homologs thereof, having improved thermoactivity, specific activity, and other desirable properties. Also provided are methods for producing ethanol and other valuable organic compounds by combining cellobiohydrolase variants with cellulosic materials.
    Type: Application
    Filed: March 12, 2013
    Publication date: March 26, 2015
    Inventors: Behnaz Behrouzian, Xinkai Xie, Kui Chan, Xiyun Zhang, Vesna Mitchell, Douglas A. Hattendorf
  • Publication number: 20150079627
    Abstract: The invention relates to processes of producing a fermentation product, comprising liquefying a starch containing material with an alpha-amylase; pre-saccharifying and/or saccharifying and fermenting using a fermentation organism in the presence of a carbohydrate source generating enzyme and a cellulolytic composition The invention also relates to methods of dewatering whole stillage.
    Type: Application
    Filed: March 28, 2013
    Publication date: March 19, 2015
    Inventors: Thomas Rasmussen, Jeremy Saunders, James Croonenberghs, Zhengfang Kang, Joyce Craig, Michael John Akerman
  • Publication number: 20150079651
    Abstract: A method to recover and harvest nutrients and volatile gases such as alcohols from a liquid stream using a fixed film bioreactor. The method includes a means of concentrating product gas stripped from a bioreactor.
    Type: Application
    Filed: November 20, 2014
    Publication date: March 19, 2015
    Inventor: Dennis A. Burke