Multiple Stages Of Fermentation; Multiple Types Of Micro-organisms Or Reuse Of Micro-organisms Patents (Class 435/162)
  • Patent number: 10752924
    Abstract: Methods for the recovery of lipids, sugars, and proteins from microbial biomass by enzymatic digestion are disclosed. The methods involve treating microalgae with a fungal acid protease, or with a mixture of at least one protease and at least one amylase.
    Type: Grant
    Filed: August 29, 2014
    Date of Patent: August 25, 2020
    Assignee: The University of Toledo
    Inventors: Agasteswar K. Vadlamani, Patricia Relue, Sridhar Viamajala, Heng Shao, Sasidhar Varanasi
  • Patent number: 10699406
    Abstract: A method of determining a plant's biomass includes three steps, namely establishing an X-ray photograph of the plant, establishing an absorption characteristic of the plant in an X-ray photograph, and determining the plant's biomass by means of the absorption characteristic of the plant. Said determining is based on a predetermined relation between a reference absorption characteristic and a reference biomass.
    Type: Grant
    Filed: March 16, 2018
    Date of Patent: June 30, 2020
    Assignee: Fraunhofer-Gesellschaft zur Förderung der angewandten Forschung e.V.
    Inventors: Stefan Gerth, Norman Uhlmann, Joelle Claussen
  • Patent number: 10584359
    Abstract: Disclosed is a genetically recombinant Saccharomyces cerevisiae useful for degrading and utilizing kitchen wastes. Genes encoding ?-amylase (AMY), glucoamylase (GA) and acid protease (AP) were introduced into the genetically recombinant Saccharomyces cerevisiae using a Saccharomyces cerevisiae multi-gene co-expression vector and successfully expressed and secreted. The Saccharomyces cerevisiae so obtained are capable of secreting amylases and protease to degrade the starch and proteins in kitchen wastes to produce carbon and nitrogen sources such as glucose, polypeptides and amino acids, allowing fermentation into ethanol.
    Type: Grant
    Filed: March 21, 2014
    Date of Patent: March 10, 2020
    Assignee: Guangdong Recyclean Low-Carbon Technology
    Inventors: Zehuan Liu, Long Fang, Kai Yan, Xiaolong Kang, Yangyang Zheng, Renhuai Liu, Jianghai Lin, Wenjuan Xiao, Jingbo Li, Yingxue Gong
  • Patent number: 10407698
    Abstract: The present invention relates to processes of producing a fermentation product from readily fermentable sugar-material in a fermentation vat comprising a fermentation medium, comprising: feeding the readily fermentable sugar-material into the fermentation vat comprising a slurry of fermenting organism; fermenting the readily fermentable sugar material into a desired fermentation product, wherein protease is added during or after feeding of the readily fermentable sugar-material into fermentation vat or during fermentation of the readily fermentable sugar-material into the desired fermentation product. The invention also related to the use of protease for reducing foaming in the fermentation yells generating by the fermenting organism during fermentation of the readily fermentable sugar-material.
    Type: Grant
    Filed: June 19, 2014
    Date of Patent: September 10, 2019
    Assignee: NOVOZYMES A/S
    Inventors: Felipe Senne de Oliveira Lino, Thiago O. Basso, Soren Nymand-Grarup, Camila do Nascimento
  • Patent number: 10344342
    Abstract: A method of producing a saccharified solution, in which saccharified slurry is obtained by hydrothermally treating cellulosic biomass slurry in a supercritical state or a subcritical state by a continuous reactor. The method reduces the risk of a pressure reducing valve becoming clogged when the high-temperature and high-pressure slurry is flash-evaporated after being hydrothermally treated. Also provides an apparatus for producing a saccharified solution and a continuous reactor. At the time of hydrothermally treating the cellulosic biomass slurry by the continuous reactor continuously, the slurry is intensely stirred in a front part of the continuous reactor, and stirred in a rear part of the continuous reactor by a stirring device with low propelling force, such that the plug flow in the axial direction is kept while the slurry is being uniformly mixed with respect to the cross section perpendicular to the mixing axis.
    Type: Grant
    Filed: December 19, 2014
    Date of Patent: July 9, 2019
    Assignee: KAWASAKI JUKOGYO KABUSHIKI KAISHA
    Inventors: Hiromasa Kusuda, Noriaki Izumi, Hironori Tajiri, Shoji Tsujita, Masaki Tsuzawa
  • Patent number: 10280437
    Abstract: A method for producing ethanol, which is a method for producing ethanol from a saccharide obtained from biomass, includes a step of adding an additive solution containing at least acetic acid, formic acid, furfural, and hydroxymethylfurfural to the saccharide to prepare a mixed solution containing the saccharide and the additive solution, and a step of adding a microorganism to the mixed solution to ferment the saccharide using the microorganism, thereby producing ethanol.
    Type: Grant
    Filed: December 12, 2013
    Date of Patent: May 7, 2019
    Assignee: JGC CORPORATION
    Inventors: Makoto Ikeo, Shohei Okino
  • Patent number: 10190086
    Abstract: The present invention relates to methods and systems of pitching yeast to fermentation reactors. More particularly, the present invention involves pitching yeast from one fermentation tank to at least one additional fermentation tank. Advantageously, yeast can be continuously pitched from fermentor to fermentor for as long as practically desirable.
    Type: Grant
    Filed: June 17, 2015
    Date of Patent: January 29, 2019
    Assignee: POET Research, Inc.
    Inventors: Neelakantam V. Narendranath, Stephen M. Lewis, Neil D. Anderson
  • Patent number: 10066217
    Abstract: Mutant thermophilic organisms that consume a variety of biomass derived substrates are disclosed herein. Strains of Thermoanaerobacterium saccharolyticum with acetate kinase and phosphotransacetylase expression eliminated are disclosed herein. Further, strain ALK1 has been engineered by site directed homologous recombination to knockout both acetic acid and lactic acid production. Continuous culture involving a substrate concentration challenge lead to evolution of ALK1, and formation of a more robust strain designated ALK2. The organisms may be utilized for example in thermophilic SSF and SSCF reactions performed at temperatures that are optimal for cellulase activity to produce near theoretical ethanol yields without expressing pyruvate decarboxylase.
    Type: Grant
    Filed: May 1, 2007
    Date of Patent: September 4, 2018
    Assignee: THE TRUSTEES OF DARTMOUTH COLLEGE
    Inventors: Arthur Josephus Shaw, IV, Sunil G. Desai, Lee R. Lynd, Kara Podkaminer, John Bardsley, David Anthony Hogsett
  • Patent number: 9961921
    Abstract: Edible residues of ethanol production, e.g., distillers grains and solubles, are produced that are low in, or substantially free from, antibiotic residues. Antibiotics or bacteria present in edible residues resulting from ethanol production are inactivated by irradiating the edible residues.
    Type: Grant
    Filed: April 5, 2012
    Date of Patent: May 8, 2018
    Assignee: Xyleco, Inc.
    Inventor: Marshall Medoff
  • Patent number: 9879296
    Abstract: The present invention relates to a method for preparing tagatose from residue after extracting coffee, comprising: obtaining a hydrolysate by hydrolyzing residue after extracting coffee into acid; obtaining a hydrolysate refined by decolorization and ion-refining the obtained hydrolysate; and obtaining tagatose by isomerizing the refined hydrolysate to an arabinose isomerase.
    Type: Grant
    Filed: November 27, 2014
    Date of Patent: January 30, 2018
    Assignee: CJ CHEILJEDANG CORPORATION
    Inventors: Young Mi Lee, Seong Bo Kim, Min Hae Kim, Sung Jae Yang, Seung Won Park
  • Patent number: 9822382
    Abstract: A process for making ethanol by fermentation comprises the steps fermenting a mash comprising a fermentable sugar with a yeast to form a fermented mash comprising ethanol, separating a yeast concentrate from the fermentation mash, adding a mineral acid to the yeast concentrate to provide an acidified yeast concentrate having a pH between 1.8 and 3.0, adding a peroxycarboxylic acid to the acidified yeast concentrate in an amount of from 5 to 80 ppm by weight to provide a treated yeast concentrate, and fermenting a mash comprising a fermentable sugar with addition of treated yeast concentrate to form a fermented mash comprising ethanol.
    Type: Grant
    Filed: January 20, 2015
    Date of Patent: November 21, 2017
    Assignee: Evonik Degussa GmbH
    Inventor: Regina Tie Kawai Shikishima
  • Patent number: 9689003
    Abstract: A dry grind ethanol production process and system with front end milling method is provided for improving alcohol and/or by-product yields, such as oil and/or protein yields. In one example, the process includes grinding corn kernels into particles then mixing the corn particles with a liquid to produce a slurry including oil, protein, starch, fiber, germ, and grit. Thereafter, the slurry is subjected to a front end milling method, which includes separating the slurry into a solids portion, including fiber, grit, and germ, and a liquid portion, including oil, protein, and starch, then milling the separated solids portion to reduce the size of the germ and grit and release bound starch, oil, and protein from the solids portion. The starch is converted to sugar, and alcohol is produced therefrom then recovered. Also, the fiber can be separated and recovered. Oil and protein may be separated and recovered as well.
    Type: Grant
    Filed: April 20, 2015
    Date of Patent: June 27, 2017
    Assignee: Lee Tech LLC
    Inventor: Chie Ying Lee
  • Patent number: 9677036
    Abstract: An ethanol production facility is provided for processing a plurality of fermentable feedstock materials into an ethanol end product through fermentation and distillation. The facility includes a fermenting stage for converting the plurality of fermentable feedstocks into an alcohol mixture including ethanol and water, and a distillation stage. The distillation stage distills the low concentration alcohol mixture into a high concentration alcohol distillate. A kernel feedstock processing stage is receives and processes a kernel containing feedstock, and includes a receiving station for receiving the kernel containing feedstock. A storage station is provided for storing the kernel based feedstock, and a mill is provided for milling the kernel based feedstock into a flour based feedstock. A conveyor is provided for conveying the milled floor based feedstock to the fermenting stage.
    Type: Grant
    Filed: March 21, 2013
    Date of Patent: June 13, 2017
    Assignee: CENTRAL INDIANA ETHANOL, LLC
    Inventors: Gary Drook, Eric Utterback, Ryan H Drook, Randy Baer, Jeff Harts, Jason Drook
  • Patent number: 9670444
    Abstract: An ethanol production facility is provided for processing a plurality of fermentable feedstock materials into an ethanol end product through fermentation and distillation. The facility includes a fermenting stage for converting the plurality of fermentable feedstocks into an alcohol mixture including ethanol and water, and a distillation stage. The distillation stage distills the low concentration alcohol mixture into a high concentration alcohol distillate. A kernel feedstock processing stage is receives and processes a kernel containing feedstock, and includes a receiving station for receiving the kernel containing feedstock. A storage station is provided for storing the kernel based feedstock, and a mill is provided for milling the kernel based feedstock into a flour based feedstock. A conveyor is provided for conveying the milled floor based feedstock to the fermenting stage.
    Type: Grant
    Filed: June 4, 2013
    Date of Patent: June 6, 2017
    Assignee: Central Indiana Ethanol, LLC
    Inventors: Gary Drook, Eric Utterback, Ryan H Drook, Randy Baer, Jeff Harts, Jason Drook
  • Patent number: 9617565
    Abstract: [Problem] To provide an alcoholic fermentation yeast having resistance to limonene, which is a fermentation inhibitor, and a method for producing ethanol using the alcoholic fermentation yeast. [Solution] An alcoholic fermentation yeast Saccharomyces cerevisiae (Deposition No. NITE BP-890), which has resistance to limonene and can grow in the presence of limonene at a concentration of 0.1-0.5 wt %, and a method for producing ethanol using the alcoholic fermentation yeast.
    Type: Grant
    Filed: November 12, 2014
    Date of Patent: April 11, 2017
    Assignees: NIPPON STEEL & SUMIKIN ENGINEERING CO., LTD., EHIME PREFECTURE
    Inventors: Natsuki Maekawa, Yasuhiko Katoh, Takafumi Kiuchi, Praneetrattananon Suthasinee, Shinji Okamoto, Kazuko Hirayama, Syunsuke Miyaoka, Hideaki Tadanobu, Masahiko Sudo
  • Patent number: 9593348
    Abstract: A method for controlling a fermentation process includes injecting a mash into a fermenter and injecting an enzymatic additive into the fermenter on a continuous basis. The enzymatic additive is injected on a continuous basis during at least two time periods: during a batch fill and after the batch fill. The method may be used to control the fermentation processes of one or more fermenters operating in parallel.
    Type: Grant
    Filed: April 15, 2014
    Date of Patent: March 14, 2017
    Assignee: ROCKWELL AUTOMATION TECHNOLOGIES, INC.
    Inventors: Srinivas Budaraju, James F. Bartee
  • Patent number: 9528104
    Abstract: Methods and systems for the isomerization and fermentation of xylose and hexose sugars using an immobilized enzyme system capable of sustaining two different pH microenvironments in a single vessel are disclosed. Bilayer particles are dispersed in a mixture comprising an ionic borate source and xylose. The bilayer particles have a first region with a first enzymatic activity comprising xylose isomerase and a pH of 6 or above, and a second region having a second enzymatic activity at an acidic pH.
    Type: Grant
    Filed: July 31, 2013
    Date of Patent: December 27, 2016
    Assignee: The University of Toledo
    Inventors: Sasidhar Varanasi, Kripa Rao, Patricia Ann Relue, Dawei Yuan
  • Patent number: 9522991
    Abstract: The invention relates to a cellulose solution manufacturing method including: performing an ozonation treatment to bring a cellulose-containing material and ozone into contact with each other; and performing an alkali treatment to bring the obtained treated material and an alkali aqueous solution into contact with each other, thereby dissolving at least cellulose in the cellulose-containing material brought into contact with the ozone in the alkali aqueous solution. According to the invention, it is possible to provide a method of manufacturing a cellulose solution in which cellulose can be dissolved in a more simple manner, a method of manufacturing a cellulose precipitate in which cellulose can be recovered from the cellulose solution, and a method of saccharifying cellulose which uses the cellulose precipitate.
    Type: Grant
    Filed: September 22, 2011
    Date of Patent: December 20, 2016
    Assignees: EHIME PREFECTURAL GOVERNMENT, SEKISUI CHEMICAL CO., LTD.
    Inventors: Kenji Nakamura, Seiji Higaki, Manami Yatsuzuka, Kazuo Doyama, Kazuyoshi Iwane, Masaki Takao
  • Patent number: 9476068
    Abstract: A process is disclosed for producing a feed co-product by ethanol fermentation of plant matter comprising starch and another polysaccharide selected from the group consisting of hemicellulose and cellulose. The process comprises: (i) carrying out a primary fermentation process, distilling the primary fermentation mixture to form a primary feed co-product derived from the fermentation of the primary fermentation mixture and a primary distillate product comprising ethanol and (ii) carrying out a secondary fermentation process and distilling the secondary fermentation mixture to form the modified feed co-product and a secondary distillate product comprising ethanol.
    Type: Grant
    Filed: November 4, 2010
    Date of Patent: October 25, 2016
    Assignee: Abengoa Bioenergy New Technologies, LLC
    Inventors: Iñaki Mielgo, Patrick Mulvihill
  • Patent number: 9206449
    Abstract: A method of biologically producing p-hydroxybenzoic acid, and a method for producing p-hydroxybenzoic acid from lignin through chemical and biological conversion.
    Type: Grant
    Filed: August 14, 2013
    Date of Patent: December 8, 2015
    Assignee: SAMSUNG ELECTRONICS CO., LTD.
    Inventors: Won Jae Choi, Jong Won Byun, Jin Ho Ahn, Young Wan Ha, Joo-Hyun Seo
  • Patent number: 9194012
    Abstract: A method for producing sugars from lignocellulosic biomass is provided. Lignocellulosic biomass is composed of lignocellulosic fibers which are hollow and primarily contain cellulose, hemicellulose and lignin. Lignin is concentrated in the outer fiber wall and glues the fibers into bundles, but the inner fiber wall has a much lower concentration of lignin and has more easily accessible cellulose and hemicellulose. This method uses vacuum infusion to infuse homogeneous reagents into the lumen (hollow center) of lignocellulosic fibers to hydrolyze the hemicellulose and cellulose to produce sugars and oligomers, and then uses cycles of vacuum pressure to pump these homogeneous reagents and sugars and oligomers into and out of the lumen. Some types of reagents are dilute acids, cellulase enzymes, hemicellulase enzymes, Fenton or Fenton-like reagents, and hydrogen peroxide. These reagents are homogenized by mixing the reagents with process water using turbulent mixing to produce a homogeneous reagent.
    Type: Grant
    Filed: January 28, 2015
    Date of Patent: November 24, 2015
    Inventor: Edward Brian Hamrick
  • Patent number: 9109235
    Abstract: The present invention provides enriched polynucleotides, and enriched polypeptides having pectinase activity. The present invention also includes methods of using the polynucleotides and polypeptides described herein. For instance, the methods include producing metabolic product, such as ethanol.
    Type: Grant
    Filed: July 15, 2013
    Date of Patent: August 18, 2015
    Assignee: University of Georgia Research Foundation, Inc.
    Inventor: Joy Bethune Peterson
  • Patent number: 9102856
    Abstract: An improved method of conducting the pre-soaking step involving pre-soaking the ligno-cellulosic biomass in a liquid (water) at a temperature in the range of between 100° C. to 150° C. prior to soaking at higher temperatures. This material can then be soaked and soaked liquid filtered by nano-filtration. When nano-filtration is used, the pre-soak temperature can be in the range of 10° C. to 150° C.
    Type: Grant
    Filed: December 20, 2012
    Date of Patent: August 11, 2015
    Assignee: Beta Renewables, S.p.A.
    Inventors: Francesco Cherchi, Piero Ottonello, Simone Ferrero, Paolo Torre, Danilo De Faveri, Beatriz Rivas Torres, Liliane Tonte Rensi, Daniele Riva, Federica Bosio
  • Publication number: 20150147791
    Abstract: The engineering of a pathway converting CH4 to acetate and eventually to liquid fuels is disclosed. The engineered pathway involves an engineered reversal of the natural pathway for acetate conversion to CH4 by microbes and coupling the engineered pathway to existing and future technologies for microbial or chemical conversion of acetate to liquid fuels. In one aspect, methods for producing modified pathways and/or microbes are provided. In another aspect engineered microbes, including Methanosarcina acetivorans, that incorporate the engineered pathway are provided, which can mediate conversion of CH4 to acetate for ultimate conversion to liquid fuels. In a further aspect, specific modifications to the components of the pathway are provided.
    Type: Application
    Filed: November 21, 2014
    Publication date: May 28, 2015
    Inventors: James Gregory Ferry, Costas D. Maranas, Thomas Keith Wood
  • Publication number: 20150147786
    Abstract: A process for mechanical destructuring of starch-based biomass was developed that makes use of a short application of high compression, impact, and shearing forces. The biomass may be destructured using a specific energy input that is less than 40% of the total combustible energy of the biomass. The destructured starch-based biomass, with or without saccharification and/or in-feed glycosyl hydrolase enzymes, may be used in feed applications. The destructured starch-based may saccharified to produce syrups and fermentable sugars, and for production of products including ethanol using a biocatalyst.
    Type: Application
    Filed: November 18, 2014
    Publication date: May 28, 2015
    Inventors: KATHLEEN A CLARKSON, F Glenn Gallagher, Aaron Perelman, Luis Fernando Romero Millan, Vivek Sharma, Jayarama K. Shetty, Daniel A. Slanac, Paula Johanna Maria Teunissen
  • Patent number: 9040278
    Abstract: An ?-amylase from Bacillus subtilis (AmyE) produces significant amounts of glucose from various carbohydrate substrates, including vegetable starch, maltoheptaose, and maltotriose. Among other things, this advantageous property allows AmyE or variants thereof to be used in a saccharification reaction having a reduced or eliminated requirement for glucoamylase. The reduction or elimination of the glucoamylase requirement significantly improves the efficiency of the production of ethanol or high fructose corn syrup, for example.
    Type: Grant
    Filed: June 4, 2009
    Date of Patent: May 26, 2015
    Assignee: DANISCO US INC.
    Inventors: Luis G. Cascao-Pereira, Regina Chin, William A. Cuevas, David A. Estell, Sang-Kyu Lee, Michael J. Pepsin, Scott D. Power, Sandra W. Ramer, Carol A. Requadt, Andrew Shaw, Amr R. Toppozada, Louise Wallace
  • Patent number: 9040279
    Abstract: The present disclosure relates to a Bacillus subtilis alpha-amylase (AmyE) or its variants thereof. AmyE or its variants thereof may be used to more efficiently produce fermentable sugars from starch. Also disclosed are a composition comprising a glucoamylase and AmyE or variant thereof and a method of processing starch utilizing the described enzyme composition.
    Type: Grant
    Filed: June 4, 2009
    Date of Patent: May 26, 2015
    Assignee: DANISCO US INC.
    Inventors: Suzanne Breneman, Sung Ho Lee, Vivek Sharma, Jayarama K. Shetty
  • Publication number: 20150140629
    Abstract: The invention relates to an alcoholic fermentation process in the presence of one or more high alcohol tolerant yeast and one or more maltotriose positive yeast. The process of the present invention can be a fermentation process for the production of ethanol, for the production of beer, for the production of wine and the like, in a preferred embodiment, the present invention relates to a process for the production of ethanol in the presence of distiller's yeast and baker's yeast.
    Type: Application
    Filed: May 31, 2013
    Publication date: May 21, 2015
    Applicant: Cargill, Incorporated
    Inventors: Jean-Claude Marie Pierre De Troostembergh, Bernhard Horbach, Michael Josef Christian Kruse, Nicolas Andre Albert Meurens
  • Publication number: 20150139977
    Abstract: The invention relates to enzymes having xylanase, mannanase and/or glucanase activity, e.g., catalyzing hydrolysis of internal ?-1,4-xylosidic linkages or endo-?-1,4-glucanase linkages; and/or degrading a linear polysaccharide beta-1,4-xylan into xylose. Thus, the invention provides methods and processes for breaking down hemicellulose, which is a major component of the cell wall of plants, including methods and processes for hydrolyzing hemicelluloses in any plant or wood or wood product, wood waste, paper pulp, paper product or paper waste or byproduct. In addition, methods of designing new xylanases, mannanases and/or glucanases and methods of use thereof are also provided. The xylanases, mannanases and/or glucanases have increased activity and stability at increased pH and temperature.
    Type: Application
    Filed: October 22, 2014
    Publication date: May 21, 2015
    Inventors: David Weiner, David Blum, Alexander Varvak, Shaun Healey, Kristine Chang, Geoff Hazlewood, Thomas Todaro, Grace Desantis, Hwai Chang, Connie Jo Hansen, Scott W. Beaver, Thomas Woodward, Charles Hancock
  • Publication number: 20150140612
    Abstract: The present invention relates to methods for increasing hydrolysis of a pretreated cellulosic material, comprising subjecting the pretreated cellulosic material to a cellulolytic enzyme composition; a polypeptide having cellulolytic enhancing activity; a Peroxidase; and a nonionic surfactant and/or cationic surfactant, at conditions suitable for hydrolyzing the pretreated lignocellulosic material. The invention also relates to processes for producing a fermentation product comprising a hydrolysis step of the invention and a composition suitable for use in a method of the invention.
    Type: Application
    Filed: July 18, 2013
    Publication date: May 21, 2015
    Inventors: Ye Chen, Jiyin Liu, Terry Green, Mark V. Stevens, Hui Xu
  • Publication number: 20150140606
    Abstract: A method of producing a sugar liquid from cellulose-containing biomass includes (1) to (4): (1) subjecting a cellulose-containing biomass to a dilute sulfuric acid treatment and thereafter separating the treated cellulose-containing biomass into a dilute sulfuric acid-treated liquid and a cellulose-containing solid content; (2) adding a cellulase to the cellulose-containing solid content to hydrolyze the cellulose and thereafter obtaining a sugar liquid; (3) filtering the dilute sulfuric acid-treated liquid through a nanofiltration membrane at pH 2.5 or lower to thereby separate a sugar concentrated liquid as a retentate and at the same time recover a sulfuric acid aqueous solution as a permeate; and (4) reusing the whole amount or a part of the sulfuric acid aqueous solution obtained in (3) in the dilute sulfuric acid treatment in (1).
    Type: Application
    Filed: June 11, 2013
    Publication date: May 21, 2015
    Inventors: Atsushi Minamino, Hiroyuki Kurihara, Katsushige Yamada
  • Patent number: 9034608
    Abstract: The invention relates to a polypeptide having a mutation at one or more position corresponding to T219 of SEQ ID NO: 55, wherein the polypeptide has at least 50% sequence identity with SEQ ID NO: 55, and wherein the polypeptide has permease activity.
    Type: Grant
    Filed: October 11, 2011
    Date of Patent: May 19, 2015
    Assignee: DSM IP ASSETS, B.V.
    Inventors: Hendrik Wouter Wisselink, Antonius Jeroen Adriaan Van Maris, Jacobus Thomas Pronk, Paul Klaassen, Rene Marcel De Jong
  • Publication number: 20150135369
    Abstract: Provided herein are transgenic plants that include increased expression of a coding region encoding a PAE poly-peptide compared to a control plant. In one embodiment, a transgenic plant includes a phenotype of decreased recalcitrance, increased growth, or the combination thereof. Also provided herein are methods for generating transgenic plants, and methods for using transgenic plants. Examples of methods for using transgenic plants include, for instance, processing a transgenic plant described herein to result in a processed pulp, and exposing a plant material obtained from a plant described herein to conditions suitable for the production of a metabolic product.
    Type: Application
    Filed: March 11, 2013
    Publication date: May 14, 2015
    Inventors: Debra A. Mohnen, Ajaya K. Biswal
  • Publication number: 20150125906
    Abstract: The present invention relates to methods of degrading or converting biomass material enriched with hemicellulosic material into fermentable sugars.
    Type: Application
    Filed: January 14, 2015
    Publication date: May 7, 2015
    Inventors: Prashant Iyer, Harry Showmaker, Hui Xu, Kishore Rane
  • Publication number: 20150125908
    Abstract: A method of producing a sugar liquid from cellulose-containing biomass includes (1) to (3): (1): subjecting a cellulose-containing biomass to hydrothermal treatment and thereafter separating the treated cellulose-containing biomass into a hydrothermally-treated liquid and a cellulose-containing solid content; (2): adding a filamentous fungus-derived cellulase to the cellulose-containing solid content obtained in (1) to hydrolyze the cellulose and thereafter separating the hydrolysate into a saccharification residue and a sugar liquid; and (3): washing the saccharification residue obtained in (2) with the hydrothermally-treated liquid obtained in (1) to elute the filamentous fungus-derived cellulase adsorbed to the saccharification residue into the hydrothermally-treated liquid and thereafter obtaining a solution component comprising the filamentous fungus-derived cellulase by solid-liquid separation.
    Type: Application
    Filed: May 17, 2013
    Publication date: May 7, 2015
    Inventors: Hiroyuki Kurihara, Hiroko Ishizuka, Atsushi Minamino, Katsushige Yamada
  • Publication number: 20150125902
    Abstract: Provided are isolated polypeptides having glucoamylase activity, catalytic domains, and polynucleotides encoding the polypeptides, catalytic domains. Also provided are nucleic acid constructs, vectors and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides, catalytic domains.
    Type: Application
    Filed: August 24, 2012
    Publication date: May 7, 2015
    Applicant: Novozymes A/S
    Inventors: Tianqi Sun, Ming Li
  • Publication number: 20150125903
    Abstract: The present invention provides fungal xylanase and/or xylosidase enzymes suitable for use in saccharification reactions. The present invention provides xylanase and xylosidase enzymes suitable for use in saccharification reactions. The present application further provides genetically modified fungal organisms that produce xylanase(s) and/or xylosidase(s), as well as enzyme mixtures exhibiting enhanced hydrolysis of cellulosic material to fermentable sugars, enzyme mixtures produced by the genetically modified fungal organisms, and methods for producing fermentable sugars from cellulose using such enzyme mixtures. In some embodiments, the xylanase and xylosidase enzyme(s) are M. thermophila enzymes.
    Type: Application
    Filed: January 14, 2015
    Publication date: May 7, 2015
    Inventors: Nicholas John Agard, David Elgart, Jie Yang, Goutami Banerjee, Jeanne Bonomo Benoit, Dipnath Baidyaroy
  • Publication number: 20150118727
    Abstract: A process for obtaining high ethanol yield from the fermentation of an energy crop and for producing a nutritionally enhanced feed co-product is provided. In particular, the process includes converting non-fermentable polysaccharides in an energy crop into fermentable sugar. The fermentable sugars may be fermented into ethanol thereby enhancing the ethanol yield. In addition, separation of ethanol from the fermentation product yields a whole stillage product having enhanced protein content and reduced fiber content. The process requires little or no modification to the configuration of existing commercial ethanol facilities.
    Type: Application
    Filed: May 9, 2013
    Publication date: April 30, 2015
    Inventors: Carlos Blázquez Escudero, Pablo Gutiérrez Gómez
  • Publication number: 20150119607
    Abstract: Methods of producing renewable materials, such as biofuels, may include separating lignocellulosic feedstock into various fractions, pretreating at least one of the fractions, and further treating the pretreated fraction(s) to produce a renewable material. More particularly, an outer-most stalk tissue, or rind, of the lignocellulosic feedstock having the least-accessible carbohydrates can be separated from the leaves and pith of the feedstock. Then the easily-accessible leaves, pith, and sugars can be processed together, while the rind can either be processed separately to produce a renewable material, or turned into other products. In certain embodiments, a cane tissue fractionation system is included at a front end of a sugar mill.
    Type: Application
    Filed: April 30, 2013
    Publication date: April 30, 2015
    Applicant: BP Corporation North America Inc.
    Inventors: Joseph B. Binder, Jacob Borden, Micheal L. Chappell
  • Publication number: 20150118716
    Abstract: A method to increase ethanol production from a corn dry-mill process is described that comprises adding an enzyme preparation derived from Trichoderma reesei having cellulolytic activity to a saccharification process that includes conventional alpha amylase and glucoamylase. The addition of the cellulolytic enzyme decreases viscosity of the saccharified mash and can increase ethanol yield from a dry grind fermentation by as much as 10% or more. Specific characteristics are provided to show surprising and advantageous results of one particular preparation of cellulolytic enzymes from T. reesei.
    Type: Application
    Filed: May 3, 2013
    Publication date: April 30, 2015
    Applicant: ARCHER DANIELS MIDLAND COMPANY
    Inventors: Charles Abbas, Wu-Li Bao
  • Publication number: 20150112101
    Abstract: A method is provided in which pretreated and densified cellulosic biomass particulates can be hydrolyzed at a high solids loading rate as compared with the solids loading rate of loose hydrolysable cellulosic biomass fibers. The resulting high concentration sugar-containing stream can be easily converted to biofuels or to an entire suite of other useful bioproducts.
    Type: Application
    Filed: December 22, 2014
    Publication date: April 23, 2015
    Inventors: Bryan Bals, Farzaneh Teymouri, Timothy J. Campbell, Bruce E. Dale
  • Publication number: 20150105593
    Abstract: Embodiments of the present invention provide for production and recovery of ethanol or other volatile organic compounds, such as acetic acid, from solid biomass material. One embodiment comprises introducing a biomass material to a compartment of a solventless recovery system, wherein the biomass material contains one or more volatile organic compounds; contacting the biomass material with a superheated vapor stream in the compartment to vaporize at least a portion of an initial liquid content in the biomass material, said superheated vapor stream comprising at least one volatile organic compound; separating a vapor component and a solid component from the heated biomass material, said vapor component comprising at least one volatile organic compound; and retaining at least a portion of the vapor component for use as part of the superheated vapor stream.
    Type: Application
    Filed: December 16, 2014
    Publication date: April 16, 2015
    Inventors: Corey William RADTKE, Phillip Guy HAMILTON, Keith Michael KREITMAN
  • Publication number: 20150104835
    Abstract: A static solid state bioreactor and method of using same. The bioreactor comprises a vessel having an upper end and a lower end, the upper end having a sealable opening. A gas distribution system in communication with the upper end and the lower end of the vessel. A liquid distribution system in communication with the upper end of the vessel. A liquid recovery system in communication with the lower end of the vessel. A material removal system disposed at the lower end of the vessel for removing biomass from the vessel.
    Type: Application
    Filed: September 16, 2014
    Publication date: April 16, 2015
    Applicant: GeoSynFuels, LLC
    Inventors: J. Todd Harvey, Murray D. Bath, Glenn R. Sprenger, Edward Ogrodny
  • Publication number: 20150099284
    Abstract: The present invention relates to variants (mutants) of parent Termamyl-like alpha-amylases, which variant has alpha-amylase activity and exhibits altered stability, in particular at high temperatures and/or at low pH relative, and/or low Ca2+ to the parent alpha-amylase.
    Type: Application
    Filed: December 16, 2014
    Publication date: April 9, 2015
    Inventors: Thomas Thisted, Soren Kjaerulff, Carsten Andersen, Claus Crone Fuglsang
  • Publication number: 20150093791
    Abstract: Thermostable cellulase enzyme systems comprising at least one each of a thermostable endoglucanase, an exo-processive-endoglucanase, and a ?-glucosidase carry out the complete, coordinated hydrolysis of crystalline cellulose to monomeric glucose.
    Type: Application
    Filed: September 29, 2014
    Publication date: April 2, 2015
    Inventors: Rolf A. Prade, Hongliang Wang
  • Publication number: 20150093790
    Abstract: The disclosure provides thermostable enzymes isolated from Caldicellulosiruptor bescii and fragments thereof useful for the degradation of cellulose and/or hemicellulose, including thermostable cellulases and hemicellulases. The disclosure further provides nucleic acids encoding the thermostable enzymes of the disclosure. The disclosure also provides methods for the conversion of cellulose and hemicellulose into fermentable sugars using thermostable enzymes of the disclosure. The disclosure also provides enzyme cocktails containing multiple enzymes disclosed herein. The enzymes can be used to release sugars present in cellulose or hemicellulose for subsequent fermentation to produce value-added products.
    Type: Application
    Filed: December 20, 2011
    Publication date: April 2, 2015
    Applicant: The Board pf Trustees of the University of Illinois
    Inventors: Yejun Han, Xiaoyun Su, Dylan Dodd, Roderick I. Mackie, Issac K.O. Cann
  • Publication number: 20150087039
    Abstract: Methods for producing biofuels that involve enhanced separation of oil in co-product process streams are disclosed. In some embodiments, separation is enhanced by addition of an additive such as a surfactant, yeast and/or salt to the co-product process stream.
    Type: Application
    Filed: March 5, 2013
    Publication date: March 26, 2015
    Applicant: ABENGOA BIOENERGY NEW TECHNOLOGIES, LLC
    Inventor: Thomas Carsten Tandy
  • Publication number: 20150087031
    Abstract: The present invention relates to methods of processing lignocellulosic material to obtain hemicellulose sugars, cellulose sugars, lignin, cellulose and other high-value products. Also provided are hemicellulose sugars, cellulose sugars, lignin, cellulose, and other high-value products.
    Type: Application
    Filed: May 3, 2013
    Publication date: March 26, 2015
    Inventors: Robert Jansen, Claire Gregoire, philip Travisano, Lee Madsen, Neta Matis, Yael Har-Tal, Shay Eliahu, James Alan Lawson, Noa Lapidot, Luke Burke, Aharon M. Eyal, Timothy Allen Bauer, Hagit Sade, Paul Mcwilliams, Ziv-Vladimir Belman, Bassem Hallac, Michael Zviely, Yelena Gershinksy, Adam Carden
  • Publication number: 20150087030
    Abstract: A pretreatment composition and pretreatment process utilizing the composition is described. The pretreatment composition includes an aqueous organic solvent in combination with an alkaline component and an oxidizing agent. The pretreatment composition is useful for treating biomass as may be utilized in forming biofuel, paper, or other useful products. The biomass to be treated can include cellulosic biomass, lignocellulosic biomass, or a combination thereof.
    Type: Application
    Filed: April 1, 2013
    Publication date: March 26, 2015
    Inventors: Arpan Jain, Terry H. Walker
  • Patent number: 8986962
    Abstract: This invention provides co-cultures of photosynthetic microorganisms and biofuel producing microorganisms. In certain embodiments, polysaccharide-producing, photosynthetic microorganisms are microalgae having frustules provide a substrate on which biofuel-producing microorganisms can grow. In other embodiments, the photosynthetic microorganisms produce a lipid and the non-photosynthetic microorganisms produce a solvent in which the lipid is soluble.
    Type: Grant
    Filed: July 21, 2011
    Date of Patent: March 24, 2015
    Inventor: Pamela R. Contag