Treatment Of Micro-organisms Or Enzymes With Electrical Or Wave Energy (e.g., Magnetism, Sonic Waves, Etc.) Patents (Class 435/173.1)
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Patent number: 8815554Abstract: The present invention includes methods, devices and systems for isolating a nucleic acid from a fluid comprising cells. In various aspects, the methods, devices and systems may allow for a rapid procedure that requires a minimal amount of material and/or results in high purity nucleic acid isolated from complex fluids such as blood or environmental samples.Type: GrantFiled: February 28, 2014Date of Patent: August 26, 2014Assignee: Biological Dynamics, Inc.Inventors: Rajaram Krishnan, David Charlot, Eugene Tu, James McCanna, Lucas Kumosa, Paul Swanson, Robert Turner, Kai Yang, Irina Dobrovolskaya, David Liu
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Patent number: 8815555Abstract: The present invention includes methods, devices and systems for isolating a nucleic acid from a fluid comprising cells. In various aspects, the methods, devices and systems may allow for a rapid procedure that requires a minimal amount of material and/or results in high purity nucleic acid isolated from complex fluids such as blood or environmental samples.Type: GrantFiled: March 7, 2014Date of Patent: August 26, 2014Assignee: Biological Dynamics, Inc.Inventors: Rajaram Krishnan, David Charlot, Eugene Tu, James McCanna, Lucas Kumosa, Paul Swanson, Robert Turner, Kai Yang, Irina Dobrovolskaya, David Liu
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Publication number: 20140234938Abstract: Systems and methods for the activation of species and/or the delivery of species to a target environment using harmonic generation materials are generally described.Type: ApplicationFiled: February 20, 2013Publication date: August 21, 2014Applicants: Children's Medical Center Corporation, Massachusetts Institute of TechnologyInventors: Robert S. Langer, Daniel S. Kohane, Aoune Barhoumi
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Patent number: 8802082Abstract: Compositions and methods for reducing or preventing graft-versus-host disease associated with allogeneic stem cell transplantation are described. A method includes harvesting hematopoietic cells from a patient with an immunologic disorder and harvesting hematopoietic cells from a donor. The harvested hemaptopoietic cells from the patient and the donor are mixed ex vivo for a period of time sufficient to activate lymphocytes within the hematopoietic cells harvested from the donor such that an immune reaction occurs and an activated portion and an unactivated portion is produced. A rhodamine B derivative is then added to the mixture of cells and the mixture is irradiated at a suitable wavelength and intensity for the selective destruction and/or inactivation of the activated portion without substantially affecting the unactivated portion or causing systemic toxicity in said patient. The irradiated cells are then infused into the patient.Type: GrantFiled: March 29, 2013Date of Patent: August 12, 2014Assignees: Universite de Montreal, Hopital Maisonneuve-RosemontInventors: Denis-Claude Roy, Martin Guimond, Nestor Molfino, Luc Villeneuve
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Patent number: 8802006Abstract: In one aspect, there is provided assembling an analyte sensor with an analyte sensor insertion device, packaging the assembled analyte sensor and sensor insertion device in a substantially airtight seal, and irradiating the packaged assembled analyte sensor and sensor insertion device at a predetermined dose using one or more electron beam accelerators.Type: GrantFiled: August 27, 2012Date of Patent: August 12, 2014Assignee: Abbott Diabetes Care Inc.Inventors: Christopher Allen Thomas, Jasmin Y. Zhao
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Publication number: 20140220615Abstract: Engineered fusion proteins comprising photochromic protein domains are disclosed. In particular, the inventors have constructed fusion proteins containing photoswitchable photochromic fluorescent protein domains linked to selected proteins and shown that such fusion proteins can be used to control the activity or localization of selected proteins with light.Type: ApplicationFiled: April 11, 2014Publication date: August 7, 2014Applicant: Office of Technology LicensingInventors: Xin Zhou, Michael Lin
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Publication number: 20140220555Abstract: Techniques for generating microtissues, including a micro-fabricated platform including at least one micro-well including a plurality of micro-cantilevers coupled thereto and surrounded by a plurality of ridges, each micro-cantilever including a cap at a terminal end thereof. The platform can be immersed in a suspension of cells. The suspension of cells can be driven into at least one micro-well, and the ridges can be de-wetted to remove excess suspension and isolate the suspension of cells in each micro-well. The cells can be driven in the suspension of each micro-well toward a top surface of the suspension, which can be polymerized to form a matrix. The cells can be cultivated to spontaneously compact the matrix such that the micro-cantilevers anchor and constrain the contracting matrix to form a band of microtissue that spans across the micro-cantilevers.Type: ApplicationFiled: April 8, 2014Publication date: August 7, 2014Applicant: The Trustees of the University of PennsylvaniaInventors: Christopher S. Chen, Ken Margulies, Thomas Boudou, Wesley Legant, Michael T. Yang
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Publication number: 20140212943Abstract: A process is described for the treatment of stem cells or differentiated cells by application of radiofrequency electro-magnetic fields, by means of an electromagnetic field generator (10), comprising a power supply (11), at least one antenna (12) adapted to radiate a scattered electromagnetic field (13) of a power less than 100 mW, a modulator (14) associated with said generator (10) and adapted to modulate the emission thereof, and at least one convector electrode (15), adapted to be direct the radiofrequency currents induced by said electromagnetic field (13), and applied in proximity of said stem or differentiated cells.Type: ApplicationFiled: August 10, 2012Publication date: July 31, 2014Inventors: Salvatore Rinaldi, Vania Fontani
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Patent number: 8785144Abstract: Disclosed is an analysis method comprising the steps of: (a) reacting a substance to be analyzed with at least a specific partner which exhibits a selective interaction with the substance, converting a soluble substance to an insoluble substance by an insolubilization reaction, in correlation with the amount of the substance to be analyzed contained in a sample, and depositing the insoluble substance on a sensing part, and (b) electrically analyzing the insoluble substance deposited on the sensing part, wherein at least one of steps (a) and (b) is carried out under flow conditions.Type: GrantFiled: March 17, 2009Date of Patent: July 22, 2014Assignee: Mitsubishi Chemical Medience CorporationInventors: Yasuo Ifuku, Nagamoto Murai
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Patent number: 8785141Abstract: The invention aims at providing an adsorbent for bacterial toxins, a method for removal of such toxins by adsorption, and an adsorber packed with said adsorbent. Provided are an adsorbent for bacterial toxins, which comprises a water-insoluble porous material having a mode of pore radius of 20 angstroms to 1,000 angstroms, a method for removal of bacterial toxins using said adsorbent, and an adsorber packed with said adsorbent.Type: GrantFiled: August 24, 2001Date of Patent: July 22, 2014Assignee: Kaneka CorporationInventors: Tamiji Fujimoto, Fumiyasu Hirai, Shigeo Furuyoshi
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Patent number: 8778154Abstract: To provide a system by which evaluation of circumstances of contamination by microparticles having nucleic acid can be performed rapidly and accurately. The theme is achieved by a system for measuring microparticles that includes: (1) a microparticle adhesion step of adhering the microparticles having nucleic acid to a microparticle adhesion member; (2) a membrane breakage step of breaking membranes of the adhered microparticles by electrical discharge; (3) an electrophoresis step of electrophoresing the microparticles in a thickness direction of a gel to make the nucleic acid in the microparticles migrate from a negative electrode side toward a positive electrode side and adhere the nucleic acid on a surface of a nucleic acid detection member; and (4) a nucleic acid measurement step of fluorescently staining the surface of the nucleic acid detection member to measure a concentration of the nucleic acid.Type: GrantFiled: December 17, 2009Date of Patent: July 15, 2014Assignee: Toyohashi University of TechnologyInventors: Akira Mizuno, Kazunori Takashima, Hachiro Yasuda, Masudur Rahman
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Patent number: 8772004Abstract: A method and device for aggregating algae in an aqueous solution is disclosed. The method can include providing an algae feed comprising a liquid and algae dispersed therein. The algae feed can be aggregated by applying a nanosecond pulsed electric field to the algae feed. The nanosecond pulsed electric field can include a plurality of electric pulses having a pulse duration ranging from 1 to 1,000 nanoseconds. The method can also include separating an aggregated algae stream from the algae feed and feeding the aggregated algae stream to a lipid extraction operation.Type: GrantFiled: June 25, 2010Date of Patent: July 8, 2014Assignee: Old Dominion University Research FoundationInventors: Gary C. Schafran, Juergen F. Kolb, Aron Stubbins, Karl H. Schoenbach
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Publication number: 20140162338Abstract: The invention relates to a special plasma source designated as a plasma intractor (PI) for producing a cold, homogeneous plasma under atmospheric pressure conditions, which plasma source can be used advantageously to excite and control reactive processes in flowing media. The device according to the invention is characterized in that the device comprises at least 6 elongated electrodes (2) and a molded body (1) made of insulating material, the molded body (1) being provided with an elongated cylindrical cavity (7) and with additional holes, which are guided parallel to the cavity (7) and arranged symmetrical to the axis of the cavity and equidistant to one another, and the electrodes (2) are embedded in holes of the molded body (1) and are connected to an AC high-voltage supply in such a way that the polarities of respective adjacent electrodes are opposite in each phase of the voltage period.Type: ApplicationFiled: May 29, 2012Publication date: June 12, 2014Applicant: Leibniz-Institut fuer Plasmaforschung und Technologie e.V.Inventors: Jan Schaefer, Stefan Horn, Ronny Brandenburg, Ruediger Foest, Manfred Stieber, Klaus-Dieter Weltmann
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Publication number: 20140155681Abstract: Provided are methods for disrupting dipolar interactions in vivo and in vitro, as wells as treating conditions associated with abnormal cell growth, by subjecting to an artificial, stable magnetic field. Also provided are assays involving an artificial, stable magnetic field. Additionally, the exposure of an artificial, stable magnetic field may be combined with other non-magnetic therapy in treatment or assays.Type: ApplicationFiled: November 29, 2013Publication date: June 5, 2014Inventor: Jiu Xiang NI
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Publication number: 20140154237Abstract: This invention relates to methods and kits for safely removing and inactivating topical therapeutic or cosmetic compositions. The methods and kits according to the invention are particularly well suited for removing and inactivating highly toxic substances.Type: ApplicationFiled: December 22, 2011Publication date: June 5, 2014Applicant: REVANCE THERAPEUTICS, INC.Inventors: Jacob Waugh, Curtis Ruegg
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Publication number: 20140147494Abstract: An aligned polymer article including substrates, wherein the substrates are not covalently bonded to the aligned collagen and a method of forming such articles wherein substrates are mixed with a polymer in solution to form a polymer-substrate mixture. The mixture is placed in an electrochemical cell and a voltage is applied to the cell generating a pH gradient, wherein the polymer aligns in the cell and migrates to the isoelectric plane of the polymer solution.Type: ApplicationFiled: November 8, 2013Publication date: May 29, 2014Inventor: Xingguo CHENG
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Patent number: 8722393Abstract: Method and apparatus for processing a cell culture are provided. The method includes establishing a cell culture within a holding device having one or more wells, each well holding a cell culture, and including a well substrate with at least one electrode in contact with the cell culture; periodically applying at least one electrical pulse to the at least one electrode to prevent cells from attaching to and achieving confluence over the at least one electrode while allowing cells to attach to and achieve confluence over other portions of the well substrate; and discontinuing the periodically applying of the at least one electrical pulse to the at least one electrode after cells have achieved confluence over the other portions of the well substrate, and thereafter, monitoring the cell culture to monitor migration of cells over the electrode(s) from the other portions of the well substrate.Type: GrantFiled: July 23, 2012Date of Patent: May 13, 2014Assignee: Applied Biophysics, Inc.Inventors: Ivar Giaever, Charles R. Keese
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Publication number: 20140127739Abstract: The present invention relates to a device comprising an organic field effect transistor (OFET) with charge injecting contacts containing a semiconductor layer formed by a perylene derivative, to uses of said device as a medical sensor and/or as a medical cell stimulator and to methods of stimulating and/or monitoring biological cellular activity by using said device.Type: ApplicationFiled: January 6, 2014Publication date: May 8, 2014Applicant: E.T.C. S.R.L.Inventors: Raffaella CAPELLI, Stefano TOFFANIN, Valentina BENFENATI, Michele MUCCINI, Roberto ZAMBONI, Gianluca GENERALI
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Publication number: 20140128800Abstract: Provided herein is a chimeric photoactivatable polypeptide comprising an opsin membrane receptor, wherein an intracellular domain of the opsin membrane receptor is replaced with a corresponding intracellular domain of a chemokine receptor, a sphingosine-1-phosphate receptor or an ATP receptor and uses thereof. Further provided are methods of treating cancer, injury of the nervous system, autoimmune disease, and graft rejection comprising administering to the subject a cell that expresses the chimeric photoactivatable polypeptide and exposing the cell to a visible light source.Type: ApplicationFiled: June 28, 2012Publication date: May 8, 2014Applicant: UNIVERSITY OF ROCHESTERInventor: Minsoo Kim
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Patent number: 8715981Abstract: Networks of single-walled carbon nanotubes (SWCNTs) decorated with Au-coated Pd (Au/Pd) nanocubes are employed as electrochemical biosensors that exhibit excellent sensitivity (2.6 mA mM?1 cm?2) and a low estimated detection limit (2.3 nM) at a signal-to-noise ratio of 3 (S/N=3) in the amperometric sensing of hydrogen peroxide. Biofunctionalization of the Au/Pd nanocube-SWCNT biosensor is demonstrated with the selective immobilization of fluorescently labeled streptavidin on the nanocube surfaces via thiol linking. Similarly, glucose oxidase (GOx) is linked to the surface of the nanocubes for amperometric glucose sensing. The exhibited glucose detection limit of 1.3_M (S/N=3) and linear range spanning from 10 ?M to 50 mM substantially surpass other CNT-based biosensors.Type: GrantFiled: January 27, 2010Date of Patent: May 6, 2014Assignee: Purdue Research FoundationInventors: Jonathan Clay Claussen, Aaron D. Franklin, Timothy S. Fisher, D. Marshall Porterfield
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Publication number: 20140120167Abstract: Methods of treating diseases through the intracellular enhancement and synergy of chemo- and radiation-therapies by employing cancer cell-specific on-demand mechanical intracellular impact. The methods, quadrapeutics, combines four clinically validated modalities: encapsulated drugs, colloidal gold nanoparticles (GNPs), near-infrared short laser pulses, and X-rays.Type: ApplicationFiled: October 30, 2013Publication date: May 1, 2014Applicant: WILLIAM MARSH RICE UNIVERSITYInventors: Dmitri Lapotko, Katsiaryna Hleb, Michael E. Kupferman, Daniel Scudder Wagner, Vladimir Torchilin, Xiangwei Wu
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Patent number: 8709445Abstract: This invention provides compositions for inducing an immune response in a vertebrate host against a protozoan parasite. In certain embodiments the composition comprises a protozoan parasite comprising a psoralen-modified DNA, whereby said protozoan parasite is killed but metabolically active (KBMA); and optionally a Toll-like receptor agonist.Type: GrantFiled: July 31, 2008Date of Patent: April 29, 2014Assignee: Los Angeles Biomedical Research Institute at Harbor-UCLA Medical CenterInventors: Noah A. Craft, Kevin W. Bruhn, Ron A. Birnbaum
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Patent number: 8709801Abstract: The invention relates to a kit and method for the capture of tumor cells in a body fluid sample or a serum-containing sample. The kit and method of the invention can capture living tumor cells but not non-living tumor cells or cell fragments so that the tumor species can be further identified by further culture of the captured tumor cells. Also, the kit and method of the invention can readily identify whether a sample contains tumor cells and collect these tumor cells for further identification so that the presence of cancer and development of the metastasis and early relapse can be identified.Type: GrantFiled: December 29, 2009Date of Patent: April 29, 2014Assignee: Taipei Medical UniversityInventors: Richard Li-Chern Pan, Fang-Chi Hsu
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Patent number: 8709740Abstract: A device having a substrate and an enzyme attached to the substrate. The substrate has a polymeric surface having at least two conductivity states. A minimum voltage that does not cause a redox reaction in the enzyme may be applied to the polymeric surface to change the conductivity state of the surface. A method of controlling enzyme activity by providing the above substrate with polymeric surface, attaching an enzyme to the substrate, and altering the conductivity state of the polymeric surface. Changing the conductivity of the polymer can change the activity of the enzyme.Type: GrantFiled: August 11, 2005Date of Patent: April 29, 2014Assignee: The United States of America, as represented by the Secretary of the NavyInventors: Brett D. Martin, Luminita M. Velea, Banahalli Ratna, Bruce P. Gaber
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Patent number: 8703457Abstract: A cell separation apparatus comprises a means for moving a cell within a cell separating space by applying an electrical voltage to cells in the cell separating space using gel electrodes, and channels for separating and discharging the cell, which thus does not damage a cell sample and prevents the exhaustion of an electrode due to its electrolysis.Type: GrantFiled: April 30, 2004Date of Patent: April 22, 2014Assignee: On-Chip Cellomics Consortium Co., Ltd.Inventors: Kenji Yasuda, Kazunori Takahashi
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Patent number: 8703050Abstract: The present invention includes a composition for enhancing the antimicrobial efficacy of photodynamic disinfection against Gram-negative organism that is receptive to paraben potentiation using a composition comprising a photosensitizer and at least one paraben. The present invention also includes a method for photodynamic disinfection comprising applying the composition to a desired treatment area and applying light to the desired treatment area at a wavelength absorbed by the photosensitizer so as to inhibit Gram-negative organism located within the treatment area wherein the Gram-negative organism is receptive to paraben potentiation.Type: GrantFiled: October 22, 2009Date of Patent: April 22, 2014Assignee: Ondine International Ltd.Inventors: Cale Street, Lisa Pedigo, Nicolas Loebel
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Patent number: 8698031Abstract: Among other things, for use in directional motion of chiral objects in a mixture, a field is applied across the chamber and is rotating relative to the chamber to cause rotation of the chiral objects. The rotation of the objects causes them to move directionally based on their chirality. The method applies to sugars, proteins, and peptides, among other things, and can be used in a wide variety of applications.Type: GrantFiled: June 19, 2008Date of Patent: April 15, 2014Assignee: Dynamic Connections, LLCInventors: Osman Kibar, Mirianas Chachisvilis, Eugene Tu, Thomas H. Marsilje
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Patent number: 8697446Abstract: The present invention relates to a cell fusion chamber in which two types of cells having different diameters are fused, the cell fusion chamber including: a cell fusion region in which cell fusion is carried out; a pair of electrodes formed by a conductor and disposed opposite to each other in the cell fusion region; and a partition wall having at least one fine pore; near the fine pore, a cell fusion device including a cell fusion container containing a cell fusion region; a pair of electrodes; a spacer; and an insulator disposed between the spacer and one of the electrodes and having at least one fine pore; and an electronic power supply which applies an alternating voltage and a voltage pulsed direct current to the electrodes, and a cell fusion method using the same.Type: GrantFiled: September 14, 2012Date of Patent: April 15, 2014Assignee: Tosoh CorporationInventors: Toru Futami, Takahiro Maruyama, Atsushi Morimoto
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Patent number: 8692192Abstract: The present invention relates generally to mass spectrometry. The present invention relates more particularly to methods and systems for use in mass spectrometric identification of a variety of analytes, including high molecular weight species such as proteins. One embodiment of the invention is a method for analyzing an analyte. The method includes nebulizing a suspension of the analyte in a solvent with a surface acoustic wave transducer; and performing mass spectrometry on the nebulized suspension. The surface acoustic wave transducer can be used, for example, to transfer non-volatile peptides and proteins (as well as other analyztes, such as oligonucleotides and polymers) to the gas phase at atmospheric pressure. Nebulization using surface acoustic waves can be conducted in a discontinuous or pulsed mode, similar to that used in MALDI, or in a continuous mode, as in ESI.Type: GrantFiled: April 8, 2013Date of Patent: April 8, 2014Assignee: University of Washington through its Center for CommercializationInventors: David R. Goodlett, Scott R. Heron, Jon Cooper
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Publication number: 20140093482Abstract: The present invention relates to a method of preparing a population of stem cells for autologous implantation to a subject. The cells are activated by irradiating the cells with one or more wavelengths of yellow and red and/or green light. In particular, the cells are irradiated with 575-595 nm (5-20 mW), and 630-635 nm or 660-670 nm (10-100 mW) and/or 510-540 nm (10-60 mW) of monochromatic light for 30-60 mins. Preferably the stem cells are adipose-derived stem cells. Also the invention relates to therapeutic applications of the activated stem cells.Type: ApplicationFiled: October 4, 2013Publication date: April 3, 2014Applicant: AdiStem, Ltd.Inventors: Bill Paspaliaris, James Andrew Farrant Thornton
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Patent number: 8685317Abstract: Shock waves are applied to clean and sterilize fluids in containers and conveyances. Shock waves destroy pathogens and pollutants in blood, water, food liquids and other fluids.Type: GrantFiled: December 19, 2012Date of Patent: April 1, 2014Assignee: Sanuwave, Inc.Inventors: Iulian Cioanta, Yannick Spenninck
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Publication number: 20140087440Abstract: A culture apparatus and method for growing cells and tissue in a three-dimensional configuration harnesses magnetic, paramagnetic, ferromagnetic and diamagnetic forces. The cells or tissue are grown with magnetized core particles and are suspended via magnetic forces in a native, non-restricted, three-dimensional configuration while being maintained in a normal gravity (1 g) growth environment in the absence of rotational alteration of the gravity vector.Type: ApplicationFiled: December 4, 2013Publication date: March 27, 2014Applicant: UNIVERSITY OF SOUTH FLORIDAInventors: JEANNE L. BECKER, SUSAN B. COFFIN
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Removal of adenine during a pathogen reduction process in whole blood or red blood cells by dilution
Patent number: 8679736Abstract: The methods of this invention involve preventing the formation of a complex between adenine and riboflavin by reducing the amount of adenine in a solution containing blood or blood components to be pathogen reduced.Type: GrantFiled: December 17, 2009Date of Patent: March 25, 2014Assignee: Terumo BCT Biotechnologies, LLCInventors: Raymond P. Goodrich, Suzann K. Doane -
Patent number: 8679806Abstract: A real-time method employing a portable peptide-containing potentiometric biosensor, can directly detect and/or quantify bacterial spores. Two peptides for specific recognition of B. subtilis and B. anthracis Sterne may be immobilized by a polysiloxane monolayer immobilization (PMI) technique. The sensors translate the biological recognition event into a potential change by detecting, for example, B. subtilis spores in a concentration range of 0.08-7.3×104 CFU/ml. The sensing method exhibited highly selective recognition properties towards Bacillus subtilis spores over other kinds of spores. The selectivity coefficients of the sensors for other kinds of spores are in the range of 0-1.0×10?5. The biosensor method not only has the specificity to distinguish Bacillus subtilis spores in a mixture of B. subtilis and B. thuringiensis (thur.) Kurstaki spores, but also can discriminate between live and dead B. subtilis spores. Furthermore, the sensing method can distinguish a Bacillus subtilis 1A700 from other B.Type: GrantFiled: July 21, 2008Date of Patent: March 25, 2014Assignee: Polytechnic Institute of New York UniversityInventors: Kalle Levon, Bin Yu, Yanxiu Zhou
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Patent number: 8672826Abstract: The present invention relates to the in vivo stimulation of cellular material. In particular, the present invention relates to a method for stimulating cells and tissue in vivo comprising the step of applying to said cells and tissue an electrobiomimetic stimulus, wherein the growth characteristics of said cells and tissue are enhanced as compared to cells and tissue not receiving said stimulus.Type: GrantFiled: April 4, 2007Date of Patent: March 18, 2014Assignee: Global Energy Medicine Pty. Ltd.Inventors: Jeffrey David Edwards, Chin Joo Goh, Kay Fei Chan
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Patent number: 8673605Abstract: The present invention relates to a method for differentiation of mesenchymal stem cells or dental pulp stem cells. More specifically, the invention relates to a method for differentiating stem cells to neural cells by applying mesenchymal stem cells or dental pulp stem cells with a low-frequency electromagnetic field. The differentiation method according to the present invention can induce differentiation even with low-cost mediums rather than induced neural differentiation mediums which are expensive due to addition of growth factors, and the neural cells differentiated according to the present invention may be useful for treatment of neurological brain diseases.Type: GrantFiled: June 8, 2011Date of Patent: March 18, 2014Assignee: Dongguk University Industry-Academic Cooperation FoundationInventors: Jung-Keug Park, Sung Min Kim, Soo Chan Kim, Moon Young Yoon, Hyun Jin Cho, Young-Kwon Seo, Hee Hoon Yoon
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Patent number: 8669085Abstract: The present invention provides a sonoporation-based method that can be universally applied for delivery of compounds into Gram positive bacteria. Gram positive bacteria which can be transformed by sonoporation include, for example, Bacillus, Streptococcus, Acetobacterium, and Clostridium. Compounds which can be delivered into Gram positive bacteria via sonoporation include nucleic acids (DNA or RNA), proteins, lipids, carbohydrates, viruses, small organic and inorganic molecules, and nano-particles.Type: GrantFiled: January 29, 2010Date of Patent: March 11, 2014Assignee: UT-Battelle, LLCInventors: Yunfeng Yang, Yongchao Li
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Publication number: 20140065664Abstract: The invention provides Rf and more particularly microwave treatment devices for biological samples or tissue. In a preferred embodiment, the antenna are capable of directing energy to a particularly focused area under the surface of the skin of a human. In other embodiments, the flexible antenna provides efficient delivery of energy to the sample or tissue regardless of the conformation of the antenna.Type: ApplicationFiled: October 8, 2013Publication date: March 6, 2014Applicant: Renewave Medical Systems SAInventors: Gerard Aknine, Jean Daniel Richerd
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Patent number: 8663959Abstract: Methods and devices for preparing a biological sample for analysis are described. The biological sample from an organism has at least macromolecule having a primary structure that naturally degrades after the sample is removed from the organism. The method includes causing the biological sample to adopt a shape to permit rapid and uniform heating. The shaped sample is then rapidly and uniformly heated, thereby altering a secondary structure of the macromolecule while preserving its primary structure.Type: GrantFiled: August 28, 2006Date of Patent: March 4, 2014Assignee: Denator AktiebolaInventors: Karl Sköld, Marcus Svensson, Göran Palmers, Per Erik Andrén
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Publication number: 20140056822Abstract: DNA dendrimers for targeted delivery of radiation absorbing nanoparticles and thermal ablation of cells and tissues are provided. Also provided are methods of making and methods of using the DNA dendrimers.Type: ApplicationFiled: November 6, 2013Publication date: February 27, 2014Applicant: Genisphere, LLCInventors: James Kadushin, Robert C. Getts
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Publication number: 20140051664Abstract: Methods for treating cancer in a subject, comprising administering to the subject an effective amount of a compound represented by the following structural formula a tautomer, or a pharmaceutically acceptable salt thereof in combination with radiotherapy. The variables depicted in the structural formula are defined herein.Type: ApplicationFiled: February 22, 2012Publication date: February 20, 2014Applicant: SYNTA PHARMACEUTICALS CORP.Inventors: Kevin P. Foley, David Proia
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Patent number: 8653442Abstract: The invention provides a method, apparatus and system for separating cellular components, and can be combined with holographic optical trapping manipulation or other forms of optical tweezing. One exemplary method includes providing a first flow having a plurality of components; providing a second flow; contacting the first flow with the second flow to provide a first separation region; and differentially sedimenting a first cellular component of the plurality of components into the second flow while concurrently maintaining a second cellular component of the plurality of components in the first flow. The second flow having the first cellular component is then differentially removed from the first flow having the second cellular component. Holographic optical traps may also be utilized in conjunction with the various flows to move selected components from one flow to another, as part of or in addition to a separation stage.Type: GrantFiled: March 6, 2012Date of Patent: February 18, 2014Assignee: Premium Genetics (UK) LimitedInventors: Daniel Mueth, Joseph Plewa, Jessica Shireman, Amy Anderson, Lewis Gruber, Neil Harris Rosenbaum
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Publication number: 20140037755Abstract: The present invention relates to compounds and pharmaceutical compositions for treating cellular proliferative disorders, e.g., in patients having one or more p53-deficient cells, screening assays for identifying such compounds, and methods for treating such disorders.Type: ApplicationFiled: May 13, 2013Publication date: February 6, 2014Applicant: Massachusetts Institute of TechnologyInventors: Michael B. Yaffe, Isaac A. Manke, Hans Christian Reinhardt
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Patent number: 8642306Abstract: A liquid sample flow containing living cells is irradiated with measurement laser light and the photo data of at least either scattering light or fluorescence that is generated by each of the living cells in the liquid sample flow due to the irradiation with the measurement laser light is acquired. Based on the photo data thus acquired, it is determined whether each of the cells assignable to the respective photo data is an unnecessary living cell or a target living cell. Based on the determination results, a pulse voltage is then applied exclusively to the living cells having been determined as unnecessary living cells so that the unnecessary living cells are damaged and killed.Type: GrantFiled: June 11, 2008Date of Patent: February 4, 2014Assignee: Mitsui Engineering & Shipbuilding Co., Ltd.Inventors: Shigeyuki Nakada, Noriaki Kimura, Akihide Ito, Kenji Yasuda
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Patent number: 8637285Abstract: The present invention relates to a method by which a controlled source of heat (preferably a laser 3) generates a pulsating vapor bubble 4 in an enclosed liquid. The pulsating rate (the frequency) is preferably in the ultrasonic region, whereby cavitation occurs in the liquid. The cavitation effect will cause disruption of suspended components such as cells or bacterial spores. The ultrasonic disruption of cellular components is known as lysing by sonication.Type: GrantFiled: December 23, 2005Date of Patent: January 28, 2014Assignee: Memsflow ApSInventor: Tomas Ussing
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Publication number: 20140017791Abstract: Two or more biological micro-objects can be grouped in a liquid medium in a chamber. Grouping can comprise bringing into and holding in proximity or contact the micro-objects in a group, breaching the membrane of one or more of the micro-objects in a group, subjecting one or more of the micro-objects in a group to electroporation, and/or tethering to each other the micro-objects in a group. The micro-objects in the group can then be combined into a single biological object.Type: ApplicationFiled: July 12, 2013Publication date: January 16, 2014Inventors: Kevin T. Chapman, Igor Y. Khandros, Gaetan L. Mathieu, Steven W. Short, Ming C. Wu
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Patent number: 8623634Abstract: A method for producing biomass and sequestering greenhouse gas includes providing a greenhouse gas, providing light energy, and growing algae in a growth container with the greenhouse gas and the light energy. The algae can be processed into a biomass feedstock. The biomass feedstock can be converted into a fuel or specialty chemical. At least a portion of the algae can be used as a fertilizer for a biomass growth source.Type: GrantFiled: June 18, 2010Date of Patent: January 7, 2014Assignee: KiOR, Inc.Inventors: Steve Yanik, Robert Bartek
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Patent number: 8617840Abstract: Lysis/resealing process for preparing erythrocytes which contain an active ingredient (e.g. aspariginase or inositol hexaphosphate), the process comprising the following steps: (1) placing a globular concentrate in suspension in an isotonic solution having a haematocrit level which is equal to or greater than 65%, with refrigeration at from +1 to +8° C., (2) measuring the osmotic fragility based on a sample of erythrocytes from that same globular concentrate, preferably on a sample of the suspension obtained in step (1), (3) lysis and internalization procedure of the active ingredient, inside the same chamber, at a temperature which is constantly maintained at from +1 to +8° C., comprising allowing the erythrocyte suspension having a haematocrit level which is equal to or greater than 65% and a hypotonic lysis solution which is refrigerated at from +1 to +8° C.Type: GrantFiled: August 4, 2005Date of Patent: December 31, 2013Assignee: Erytech PharmaInventor: Yann Godfrin
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Publication number: 20130344559Abstract: A nerve growth chamber includes a stimulation vessel defined by one or more sidewalls; at least one anode and at least one cathode positioned in the sidewall or sidewalls of the stimulation vessel; a conductive medium in the stimulation vessel; nerve tissue in the stimulation vessel; and a signal generator connecting the at least one anode and the at least one cathode and activated to generate a periodic AC signal at a desired frequency and amplitude, the periodic AC signal traveling through the conductive medium and affecting the growth of the nerve tissue. A method in accordance with the operation of the apparatus is also provided.Type: ApplicationFiled: April 23, 2013Publication date: December 26, 2013Applicant: THE UNIVERSITY OF AKRONInventors: Erik Daniel Engeberg, Rebecca Kuntz Willits
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Publication number: 20130330315Abstract: The use of a purine nucleoside phosphorylase or nucleoside hydrolase or a vector encoding expression of one of these enzymes is detailed along with the use of a prodrug cleaved by the purine nucleoside phosphorylase or nucleoside hydrolase for the preparation of a direct injection inhibition of replicating or non-replicating targeted cells. The targeted cells do not normally express the introduced purine nucleoside phosphorylase or nucleoside hydrolase. The enzyme and prodrug are amenable to intermixing and injection as a single dose or as separate injection or administration to the targeted cells. The substance and prodrug efficacy are enhanced through exposure of the targeted cells to X-ray radiation. Administration of a prodrug regardless of administration route to the targeted cells is effective in combination with X-ray radiation therapy to kill or inhibit function of the targeted cells.Type: ApplicationFiled: February 20, 2012Publication date: December 12, 2013Applicants: SOUTHERN RESEARCH INSTITUTE, THE UAB RESEARCH FOUNDATIONInventors: William B. Parker, Eric J. Sorscher