Treatment Of Micro-organisms Or Enzymes With Electrical Or Wave Energy (e.g., Magnetism, Sonic Waves, Etc.) Patents (Class 435/173.1)
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Patent number: 9011698Abstract: A method and devices comprise a low frequency high energy ultrasound system having at least one sonotrode projecting into a reactor vessel through which the liquid passes via at least one inlet orifice and at least one outlet orifice. To avoid cavitation at the sonotrode, in a close region of the oscillation-transducing sonotrode surface a pressure/amplitude combination close to or above the pressure-amplitude characteristic line is generated at which considerably reduced or no cavitation occurs and in the adjacent region in the vessel at least in a region and at least at times a pressure/amplitude combination is maintained below the pressure-amplitude characteristic line at which cavitation is generated. A device has an inlet orifice arranged such that the liquid impacts directly onto the oscillation-transducing sonotrode surface, and is shaped that in the close region of the oscillation-transducing sonotrode surface a pressure close to or above the pressure-amplitude characteristic line prevails.Type: GrantFiled: November 28, 2006Date of Patent: April 21, 2015Assignee: Dr. Hielscher GmbHInventors: Harald Hielscher, Holger Hielscher, Thomas Hielscher
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Publication number: 20150105286Abstract: In this invention, a novel protein interaction domain is provided along with several of its variants. This domain is involved in protein-protein interactions with the Bcl-2 family of proteins. It is named BLID (Bcl2 family of proteins Like Interaction Domain). Several BLID peptides that could be useful for discovery of drugs to help fight pathological states like cancer are presented.Type: ApplicationFiled: October 7, 2014Publication date: April 16, 2015Inventor: Carlos Witte-Hoffmann
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Publication number: 20150104844Abstract: A material comprising positively and negatively charged nanoparticles, wherein one of said nanoparticles contained a magnetically responsive element, are combined with a support molecule, which is a long natural or synthetic molecule or polymer to make a magnetic nanoparticle assembly. When the magnetic nanoparticle assembly is combined with cells, it will magnetize those cells. The magnetized cells can then be washed to remove the magnetic nanoparticle assembly and the magnetized cells manipulated in a magnetic field.Type: ApplicationFiled: November 7, 2014Publication date: April 16, 2015Inventor: Glauco R. SOUZA
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Patent number: 9005941Abstract: The present invention includes methods, devices and systems for isolating a nucleic acid from a fluid comprising cells. In various aspects, the methods, devices and systems may allow for a rapid procedure that requires a minimal amount of material and/or results in high purity nucleic acid isolated from complex fluids such as blood or environmental samples.Type: GrantFiled: September 4, 2014Date of Patent: April 14, 2015Assignee: Biological Dynamics, Inc.Inventors: Rajaram Krishnan, David Charlot, Eugene Tu, James McCanna, Lucas Kumosa, Paul D. Swanson, Robert Turner, Kai Yang, Irina Dobrovolskaya, David Liu
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Patent number: 9005604Abstract: A scaffold assembly and related methods of manufacturing and/or using the scaffold for stem cell culture and tissue engineering applications are disclosed which at least partially mimic a native biological environment by providing biochemical, topographical, mechanical and electrical cues by using an electroactive material. The assembly includes at least one layer of substantially aligned, electrospun polymer fiber having an operative connection for individual voltage application. A method of cell tissue engineering and/or stem cell differentiation uses the assembly seeded with a sample of cells suspended in cell culture media, incubates and applies voltage to one or more layers, and thus produces cells and/or a tissue construct.Type: GrantFiled: December 15, 2010Date of Patent: April 14, 2015Assignee: The United States of America as represented by the Administrator of the National Aeronautics and Space AdministrationInventors: Lisa A. Scott-Carnell, Emilie J. Siochi, Nancy M. Holloway, Kam W. Leong, Karina Kulangara
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Patent number: 9005670Abstract: The present invention provides methods for treating cancer comprising inhibiting the activity of p300/CBP histone acetyltransferase (HAT). Also provided are p300/CBP HAT inhibitors for treating a subject having cancer. In addition, the present invention includes biomarkers for p300/CBP HAT inhibition, which are used to i) monitor the effectiveness of cancer therapy, and ii) identify anti-cancer agents for use in combination therapy.Type: GrantFiled: January 5, 2011Date of Patent: April 14, 2015Assignee: The Johns Hopkins UniversityInventors: Rhoda Myra Alani, Philip A. Cole, Gai Yan, Erin M. Bowers
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Patent number: 9000357Abstract: An apparatus and method to identify at least one component from a plurality of components in a fluid mixture, includes a first input channel containing the fluid mixture of components; at least one buffer input channel, into which at least one additional flow of buffer solution is introduced; a plurality of regions disposed at the other end of the apparatus, which are adapted to receive outputs of at least one selected component of the plurality of components, the selected component which is selectively removed from the first flow to one of the regions; a waste channel through which unselected components are removed from the first flow; a plurality of pumps connected to at least one reservoir, to control flow rates of the first flow and the additional flow(s); and a computer which controls a selection of one of the plurality of components from the fluid mixture.Type: GrantFiled: June 27, 2014Date of Patent: April 7, 2015Assignee: Premium Genetics (UK) Ltd.Inventors: Daniel Mueth, Joseph Plewa, Jessica Shireman, Amy Anderson, Lewis Gruber, Neil Rosenbaum
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Patent number: 8999714Abstract: Certain embodiments disclosed herein include, but are not limited to, at least one of compositions, methods, devices, systems, kits, or products regarding rejuvenation or preservation of stem cells. Certain embodiments disclosed herein include, but are not limited to, methods of modifying stem cells, or methods of administering modified stem cells to at least one biological tissue.Type: GrantFiled: October 12, 2012Date of Patent: April 7, 2015Assignee: The Invention Science Fund I, LLCInventors: Roderick A. Hyde, Lowell L. Wood, Jr.
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Publication number: 20150093416Abstract: Autologous anti-cancer vaccines and methods of manufacture and treatment are provided, including expansion of individual patient-derived tumor cells in an immune-compromised animal(s) to attain, quantitatively and qualitatively, sufficient material for efficacious vaccine production and utilization, to elicit an immune response against micrometastases and/or recurrence in the individual patient following tumor excision.Type: ApplicationFiled: September 25, 2014Publication date: April 2, 2015Inventor: Michael G. Hanna, JR.
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Publication number: 20150093367Abstract: A system and method are provided that use the meridian flow defined in traditional Chinese medicine to target specific regions of the body to treat certain health conditions. A cellular mixture is injected into the body via an acupoint on a particular meridian in order to deliver the mixture to an organ or body region associated with the particular meridian. The cellular mixture can be a stem cell/PRP mixture.Type: ApplicationFiled: September 29, 2014Publication date: April 2, 2015Inventor: BILL AKPINAR
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Publication number: 20150093365Abstract: Provided herein are compositions containing photoactivatable caged tamoxifen and tamoxifen derivative molecules. Also provided are kits containing one of these compositions and a light source. Also provided are methods of optically inducing nuclear translocation of a fusion protein containing a mammalian estrogen receptor ligand binding domain in a eukaryotic cell and methods of optically inducing recombination in a eukaryotic cell that include contacting a eukaryotic cell with at least one of these compositions. Also provided are methods of treating breast cancer in a subject that include administering a photoactivatable caged tamoxifen or tamoxifen derivative molecule to a subject having breast cancer.Type: ApplicationFiled: March 14, 2013Publication date: April 2, 2015Inventors: Xin Lu, Sarit S. Agasti, Ronald A. DePinho, Ralph Weissleder
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Patent number: 8993292Abstract: Methods are provided for differential extraction of DNA from at least two different cell types. Systems for carrying out the differential extraction methods are also provided. A kit is also provided for differential extraction of DNA from at least two different cell types using a multi-compartment container.Type: GrantFiled: May 8, 2008Date of Patent: March 31, 2015Assignee: Applied Biosystems LLCInventor: Yingjie Liu
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Patent number: 8986607Abstract: A validatable method for determining a photochemically effective dose for inactivating pathogens in a fluid sample is described herein. In particular, the instant invention covers methods for determining a photochemically effective dose sufficient to inactivate pathogens in a biological sample while leaving biologically active substances of interest unaffected. A batch irradiation reactor effective for inactivating pathogens in biological samples is also described.Type: GrantFiled: February 27, 2004Date of Patent: March 24, 2015Assignees: Baxter International Inc., Baxter Healthcare SAInventors: Heinz Anderle, Peter Matthiessen, Hans-Peter Schwarz, Peter Turecek, Thomas Kreil, Daniel R. Boggs
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Publication number: 20150072394Abstract: The present disclosure provides opsins, including variant opsins with increased activity and/or increased trafficking to the plasma membrane. The opsins are useful in therapeutic and screening applications, which are also provided.Type: ApplicationFiled: December 12, 2012Publication date: March 12, 2015Inventors: Karl Deisseroth, Feng Zhang
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Patent number: 8975065Abstract: Methods and devices of performing reactions for which presence of light is desirable are provided. Biological or chemical materials such as algae are put in a chamber shaped as a meandering fluid channel. The algae can be combined with biomass such as human or animal waste and then subject to light, such as natural light or light coming from a LED, to produce fuel. Production of fuel can be optimized by controlling the height-to-width ratio of the channels.Type: GrantFiled: July 23, 2007Date of Patent: March 10, 2015Assignee: California Institute of TechnologyInventors: George Maltezos, Axel Scherer
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Patent number: 8969059Abstract: The present invention includes methods, devices and systems for isolating a nucleic acid from a fluid comprising cells. In various aspects, the methods, devices and systems may allow for a rapid procedure that requires a minimal amount of material and/or results in high purity nucleic acid isolated from complex fluids such as blood or environmental samples.Type: GrantFiled: February 28, 2014Date of Patent: March 3, 2015Assignee: Biological Dynamics, Inc.Inventors: Rajaram Krishnan, David Charlot, Eugene Tu, James McCanna, Lucas Kumosa, Paul Swanson, Robert Turner, Kai Yang, Irina Dobrovolskaya, David Liu
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Patent number: 8969058Abstract: The present invention relates to systems for releasing genetic materials from a solid medium. The present invention also relates to methods for releasing genetic materials from a solid medium. The present invention further relates to methods for isolating genetic material from a biological sample.Type: GrantFiled: December 15, 2008Date of Patent: March 3, 2015Assignee: Canon U.S. Life Sciences, Inc.Inventor: Michele R. Stone
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Publication number: 20150056678Abstract: A method of treating a disease characterized by a pathological immune response in a subject in need thereof is disclosed. The method comprises administering to the subject a therapeutically effective amount of a cell preparation which comprises dying or dead leukocytes, the dying or dead leukocytes being capable of suppressing the pathological immune response, thereby treating the disease in the subject.Type: ApplicationFiled: September 29, 2014Publication date: February 26, 2015Inventor: Dror MEVORACH
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Publication number: 20150057486Abstract: The invention provides for the use of radiation sensitizing agents in combination with radiation for the treatment of neoplasia, methods for the identification of genotype-specific radiation sensitizing agents, and methods of identifying patients who could benefit from therapy with a genotype-specific radiation sensitizing agent.Type: ApplicationFiled: August 22, 2014Publication date: February 26, 2015Inventors: Mohamed Abazeed, Matthew Meyerson, Drew Adams
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Publication number: 20150053614Abstract: The present invention relates to the use of recombinant heme-containing horseradish peroxidase isoenzyme with improved technological properties such as altered glycosylation, improved catalytic properties or improved stability and different ranges of pH optima and improved surface interactions in the treatment of waste water.Type: ApplicationFiled: February 14, 2013Publication date: February 26, 2015Inventors: Martin Kulterer, Victoria Reichel, Volker Ribitsch, Anton Glieder, Florian Krainer
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Publication number: 20150041457Abstract: Systems and methods include directing limited frequencies of microwave signals toward target molecules, driving a motion of the target molecules to impact molecular recognition. In one implementation, a microwave spectra associated with the rotational modes of a target molecule is obtained. From peaks in the spectra, a mode of molecular movement is identified and a microwave signal profile is generated for driving a motion of binding portions of the molecule associated with the identified mode. Microwave signals are generated based on the signal profile for output by antennas. For example, the microwave signals can be can be used to impede the binding of quorum sensing molecules by receptors of P. aeruginosa. In one implementation, the antennas can be placed in a catheter for placement in a patient. In another implementation, the antennas can be placed in a sleeve or other device for use adjacent to the skin of a patient.Type: ApplicationFiled: February 14, 2013Publication date: February 12, 2015Inventors: William Hunt, Kyle Spencer Davis, Michelle LaPlacca, Chris Ward, John Alexander Herrmann
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Publication number: 20150037863Abstract: A method of forming a multilayer aggregate of objects in a channel including a liquid, the method including: a) providing objects at first and second superposed regions of the channel, b) obtaining first and second aggregates of objects, optionally by applying transverse acoustic waves, preferably stationary waves, within each region to objects, and c) bringing the first and second aggregates into contact to form the multilayer aggregate of objects by submitting the first and second aggregates to: gravity in absence of acoustic waves, or to acoustic waves, optionally stationary waves, inducing displacement of the first and second aggregates toward each other.Type: ApplicationFiled: February 5, 2013Publication date: February 5, 2015Inventors: Despina Bazou, Mauricio Hoyos, Luz Angelica Castro Camacho
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Patent number: 8945894Abstract: A method for directing, enhancing, and accelerating mesenchymal stem cell functions using alternating electric current. Mesenchymal stem cells are preferentially directed to either osteoblast or chondrocyte lineages, but not to the adipocyte lineage. when exposed to alternating electric current.Type: GrantFiled: September 28, 2012Date of Patent: February 3, 2015Inventors: Courtney M. Creecy, Rena Bizios
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Publication number: 20150024008Abstract: Disclosed are cancer vaccines comprising senescent cells and methods of using and preparing the vaccines.Type: ApplicationFiled: November 21, 2012Publication date: January 22, 2015Inventors: Stephen Kron, Ralph R. Weichselbaum, Elena Efimova, Yuru Meng, Amy Flor
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Patent number: 8936935Abstract: The present invention relates to a population of monodisperse magnetic nanoparticles with a diameter between 1 and 100 nm which are coated with a layer with hydrophilic end groups. Herein the layer with hydrophilic end groups comprises an inner layer of monosaturated and/or monounsaturated fatty acids bound to said nanoparticles and bound to said fatty acids, an outer layer of a phospholipid conjugated to a monomethoxy polyethyleneglycol (PEG) comprising a hydrophilic end group, or comprises a covalently bound hydrophilic layer bound to said nanoparticles.Type: GrantFiled: May 19, 2011Date of Patent: January 20, 2015Assignees: IMEC, Katholieke Universiteit Leuven, K.U. Leuven R&DInventors: Deepak Balaji Thimiri Govinda Raj, Liesbet Lagae, Wim Annaert, Gustaaf Borghs
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Patent number: 8933395Abstract: The present invention provides a method and apparatus to identify at least one component from a plurality of components in a fluid mixture, the apparatus including a first input channel into which a first flow containing a plurality of components is introduced; a plurality of buffer input channels, into which additional flows of buffer solution are introduced, disposed on either side of the first input channel; wherein the first flow and the additional flows have a flow direction along a length of the apparatus; a detector apparatus which detects and identifies selected components of the plurality of components; a laser which emits a laser beam which damages or kills selected components of the plurality of components; and at least one channel disposed at the another end of the apparatus which is adapted to receive the first flow and the additional flows after operation of the laser on the selected components.Type: GrantFiled: January 31, 2014Date of Patent: January 13, 2015Assignee: Premium Genetics (UK) Ltd.Inventors: Daniel Mueth, Joseph Plewa, Jessica Shireman, Amy Anderson, Lewis Gruber, Neil Rosenbaum
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Patent number: 8932815Abstract: The present invention includes methods, devices and systems for isolating a nucleic acid from a fluid comprising cells. In various aspects, the methods, devices and systems may allow for a rapid procedure that requires a minimal amount of material and/or results in high purity nucleic acid isolated from complex fluids such as blood or environmental samples.Type: GrantFiled: June 20, 2014Date of Patent: January 13, 2015Assignee: Biological Dynamics, Inc.Inventors: Rajaram Krishnan, David J. Charlot, Eugene Tu, James McCanna, Lucas Kumosa, Paul D. Swanson, Robert Turner, Kai Yang, Irina Dobrovolskaya, David Liu, Juan Pablo Hinestrosa Salazar, Juscilene Menezes
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Patent number: 8931490Abstract: The present invention relates to signaling mechanisms that transduce magnetic inputs into physiological cellular outputs. More particularly, the present invention relates to systems and methods for non-invasively controlling cellular signaling functions and behaviors by harnessing receptor-mediated and intracellular molecular-mediated signal transduction using nanomagnetic cellular switches.Type: GrantFiled: April 9, 2008Date of Patent: January 13, 2015Assignee: Children's Medical Center CorporationInventors: Donald E. Ingber, Robert J. Mannix, Sanjay Kumar
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Patent number: 8927508Abstract: There are provided methods of generating antibodies in a mammal against recombinant antigens using DNA plasmids capable of expressing said antigens in cells of said mammal, comprising: injecting into tissue of said mammal a DNA plasmid comprising an encoding sequence operably linked to a promoter, electroporating said tissue with an electroporation device capable of delivering an electrical pulse effective to electroporate cells of said tissue to allow entry of said DNA plasmid and expression of said antigen, and allowing said mammal to respond to said expressed antigen in order to generate antibodies to said antigen. Furthermore, there are provided methods of isolating antibodies specific against desired antigens wherein said antibodies are generated in a mammal using DNA plasmids capable of expressing said antigens.Type: GrantFiled: November 14, 2008Date of Patent: January 6, 2015Assignee: VGX Pharmaceuticals, Inc.Inventors: Ruxandra Draghia-Akli, Amir S. Khan
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Patent number: 8926815Abstract: Method for the selection or the processing of first particles sensitive to the application of an external stimulus including the step of producing, through the application of the external stimulus, the permeabilization of at least a selected first particle, consisting in the organization of the first particles through a first force field, to generate a second force field substantially placed in proximity of at least a selected first particle to be permeabilized.Type: GrantFiled: April 13, 2007Date of Patent: January 6, 2015Assignee: Silicon Biosystems S.p.AInventors: Mélanie Abonnenc, Nicoló Manaresi, Gianni Medoro
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Patent number: 8921041Abstract: The present invention includes devices and methods for transfecting a cell or cell population and dynamic monitoring of cellular events. A variety of microelectronic devices are provide that incorporate functions such as electroporation, modulation of a transmembrane potential and dynamic monitoring of cellular functions and mechanisms.Type: GrantFiled: May 17, 2012Date of Patent: December 30, 2014Assignee: ACEA Biosciences, Inc.Inventors: Xiaobo Wang, Yama A. Abassi, Josephine Atienza, Xiao Xu, Junquan Xu
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Publication number: 20140377831Abstract: The invention provides a method of enhancing a directional migration of stem cells, comprising providing one or more stem cell(s) and irradiating the stem cells with an effective energy of green light thereby enhancing stem cell directionally migrating, wherein the orientation of migration of stem cells is opposite to the green light source. Also provided is a stem cell treated by the method of the invention and a device for enhancing a directional migration of stem cells.Type: ApplicationFiled: October 31, 2013Publication date: December 25, 2014Applicant: TAIPEI MEDICAL UNIVERSITYInventor: JENNIFER HUI-CHUN HO
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Publication number: 20140377832Abstract: The invention relates to induction of reprogramming of somatic cells, by methods which require mild growth conditions. Disclosed are methods of inducing dedifferentiation of mesenchymal stromal cell (MSC), by seeding or incubating mesenchymal stromal cells (MSCs) at low density, and without introduction or expression of exogenous genes in the cells.Type: ApplicationFiled: January 14, 2013Publication date: December 25, 2014Inventors: Dov Zipori, Ofer Shoshani
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Publication number: 20140363412Abstract: Embodiments of the disclosure relate to platelet activation and/or aggregation using electric pulses. In one embodiment, a platelet-containing sample is exposed to electric pulses. At least one of the electric pulses has a duration greater than 1 microsecond and a field strength below 50 kV/cm. In another embodiment, the electric pulses may be designed with particular field strengths and durations.Type: ApplicationFiled: June 6, 2013Publication date: December 11, 2014Inventors: Vasile Bogdan Neculaes, Antonio Caiafa, Brian Duh-Lan Lee, Nicole Evelyn LaPlante, Andrew Soliz Torres, Allen Lawrence Garner
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Patent number: 8906659Abstract: Aspects of the present invention are related to methods comprising contacting an endothelial cell in an endothelial cell population with a non-thermal plasma to release an angiogenic growth factor. The released angiogenic growth factor may induce endothelial cell proliferation. In certain embodiments, the angiogenic growth factor is fibroblast growth factor-2. Preferably, the non-thermal plasma may be an atmospheric pressure dielectric barrier discharge. Additional aspects of the present invention are directed to methods for treating a disease comprising promoting angiogenesis by contacting an endothelial cell in a endothelial cell population with a non-thermal plasma to release an angiogenic growth factor. The angiogenic growth factor may induce endothelial cell proliferation.Type: GrantFiled: March 16, 2010Date of Patent: December 9, 2014Assignee: Drexel UniversityInventors: Alisa Morss Clyne, Gennady Friedman, Alexander Fridman, Sameer Kalghatgi
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Patent number: 8901913Abstract: Among others things, techniques, systems, and apparatus are disclosed for recording electrophysiological signals. In one aspect, a microelectrode sensing device includes a printed circuit board (PCB), a chip unit electrically connected to the PCB, and a cell culture chamber positioned over the chip unit and sealed to the PCB with the chip unit between the PCB and the cell culture chamber. The chip unit includes a substrate; a conductive layer positioned over the substrate that includes one or more recording electrodes; an insulation layer positioned over the conductive layer; another conductive layer positioned over the insulation layer that includes positioning electrodes; and another insulation layer positioned over the other conductive layer. The recording and positioning electrodes are electrically independent so as to independently receive a stimulus signal at each recording electrode and positioning electrode and independently detect a sensed signal at each recording electrode.Type: GrantFiled: April 16, 2009Date of Patent: December 2, 2014Assignee: CapitalBio CorporationInventors: Jing Zhu, Zhongyao Yu, Xueling Quan, Guangxin Xiang, Yuming Hu, Wanli Xing, Jing Cheng
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Publication number: 20140342427Abstract: A selectively addressable optical biomolecular carrier and its method of use for reconfiguring gene circuits are described. One carrier is a plasmon resonant nanoantenna formed from a gold metal nanorod coated with a cationic phospholipid bilayer with an aspect ratio between 2.0 and 8.0 and plasmon resonance wavelength in the near infrared range. Biomolecules such as siRNA adhere to the carrier and are introduced into a cell. The biomolecules are released from the nanoantenna carriers with exposure to light at the plasmon resonance wavelength. The nanoantenna efficiently converts absorbed optical energy to surface localized heat releasing the biomolecules at a time determined by the user. The carrier can be used to modify gene circuits by allowing temporal control over the genes within a selected gene circuit through the optical release of interfering nucleotides. Optical silencing of endogenous genes with siRNA released from nanoantenna carriers was used to illustrate the methods.Type: ApplicationFiled: May 27, 2014Publication date: November 20, 2014Applicant: THE REGENTS OF THE UNIVERSITY OF CALIFORNIAInventors: Luke P. Lee, Somin Eunice Lee
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Patent number: 8883471Abstract: A material comprising positively and negatively charged nanoparticles, wherein one of said nanoparticles contained a magnetically responsive element, are combined with a support molecule, which is a long natural or synthetic molecule or polymer to make a magnetic nanoparticle assembly. When the magnetic nanoparticle assembly is combined with cells, it will magnetize those cells. The magnetized cells can then be washed to remove the magnetic nanoparticle assembly and the magnetized cells manipulated in a magnetic field.Type: GrantFiled: September 27, 2010Date of Patent: November 11, 2014Assignee: N3D Biosciences, Inc.Inventor: Glauco R. Souza
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Patent number: 8877469Abstract: A method for producing foams containing biological materials is described. A solid or semi-solid paste is formed by combining biologically active material with a protecting agent in an aqueous solvent. The paste formed is allowed to set, and may optionally then be apportioned into the desired shape. The paste may be frozen to allow formation of ice crystals to act as porogens. Subsequently, the paste is exposed to travelling wave radiant energy under vacuum (t-REV) for drying. This causes the solvent to boil off, leaving dried material containing the biologically active material, the protecting agent, and a relatively low water content. Biologically active materials which can be used include cells, microbial cultures, live attenuated microbes, probiotics, yeasts, enzymes, vaccines, proteins, and any heat-sensitive biological material. By directing energy via a travelling wave through a sample, good control of temperature and process conditions can be achieved.Type: GrantFiled: February 1, 2007Date of Patent: November 4, 2014Assignee: Enwave CorporationInventors: Timothy D. Durance, Parastoo Yaghmaee, Shafique Ahmad, Guopeng Zhang
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Patent number: 8877470Abstract: The present invention includes methods, devices and systems for isolating a nucleic acid from a fluid comprising cells. In various aspects, the methods, devices and systems may allow for a rapid procedure that requires a minimal amount of material and/or results in high purity nucleic acid isolated from complex fluids such as blood or environmental samples.Type: GrantFiled: March 7, 2014Date of Patent: November 4, 2014Assignee: Biological Dynamics, Inc.Inventors: Rajaram Krishnan, David Charlot, Eugene Tu, James McCanna, Lucas Kumosa, Paul Swanson, Robert Turner, Kai Yang, Irina Dobrovolskaya, David Liu
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Publication number: 20140322784Abstract: Systems and methods generally useful in medicine, cellular biology, nanotechnology, and cell culturing are discussed. In particular, at least in some embodiments, systems and methods for magnetic guidance and patterning of cells and materials are discussed. Some specific applications of these systems and methods may include levitated culturing of cells away from a surface, making and manipulating patterns of levitated cells, and patterning culturing of cells on a surface. Specifically, a method of culturing cells is presented. The method may comprise providing a plurality of cells, providing a magnetic field, and levitating at least some of the plurality of cells in the magnetic field, wherein the plurality of cells comprise magnetic nanoparticles. The method may also comprise maintaining the levitation for a time sufficient to permit cell growth to form an assembly.Type: ApplicationFiled: July 15, 2014Publication date: October 30, 2014Inventors: Glauco R. SOUZA, Renata Pasqualini, Wadih ARAP, Thomas Charles KILLIAN, Robert M. RAPHAEL, Daniel Joshua STARK
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Publication number: 20140325692Abstract: The present invention provides methods for light-dependent gene regulation using a light-responsive DNA-binding protein. Also provided are related nucleic acid molecules, and protein molecules, such as those encoding or comprising the light-responsive DNA-binding protein or DNA-binding sites recognizing the light-responsive DNA-binding protein. Kits using the present light-dependent gene regulation system are further provided by the present invention.Type: ApplicationFiled: November 16, 2012Publication date: October 30, 2014Inventors: Kevin H. Gardner, Laura B. Motta-Mena, Brian D. Zotowski
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Patent number: 8871481Abstract: The present invention includes methods, devices and systems for isolating a nucleic acid from a fluid comprising cells. In various aspects, the methods, devices and systems may allow for a rapid procedure that requires a minimal amount of material and/or results in high purity nucleic acid isolated from complex fluids such as blood or environmental samples.Type: GrantFiled: May 6, 2014Date of Patent: October 28, 2014Assignee: Biological Dynamics, Inc.Inventors: Rajaram Krishnan, David Charlot, Eugene Tu, James McCanna, Lucas Kumosa, Paul Swanson, Robert Turner, Kai Yang, Irina Dobrovolskaya, David Liu
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Publication number: 20140314810Abstract: The invention provides methods of producing vaccines directed against methicillin-resistant Staphylococcus aureus (MRSA) or Venezuelan equine encephalitis virus (VEEV), with the methods comprising culturing, harvesting and/or suspending the MRSA or VEEV in the presence of a radiation-protective composition and irradiating the MRSA with a dose of radiation sufficient to render the MRSA or VEEV replication-deficient and/or non-infective. The radiation-protective compositions used in the methods of the present invention comprise at least one nucleoside, at least one antioxidant and at least one small peptide.Type: ApplicationFiled: November 1, 2012Publication date: October 23, 2014Applicant: United States Department of Health and Human ServicesInventors: Michael Daly, Elena K Gaidamakova, Ian A. Myles, Patricia A. Valdez, Sandip K. Datta
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Patent number: 8846331Abstract: Described herein are various methods, devices and systems for performing asynchronous magnetic bead rotation (AMBR) to detect and monitor cellular growth and/or behavior. Cluster rotation of magnetic particles for AMBR is descried. In particular, described herein are systems for the parallel analysis of multiple wells of a sample plate. Also described herein are methods for controlling the illumination and imaging of rotating magnetic particles.Type: GrantFiled: August 29, 2011Date of Patent: September 30, 2014Assignee: The Regents of the University of MichiganInventors: Brandon H. McNaughton, Paivo Kinnunen, Raoul Kopelman, Alan Hunt, Roy Clarke, Irene Sinn, Remy Elbez, Theodore Albertson
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Publication number: 20140256015Abstract: An objective of the present invention is to provide a caged compound that can be photoactivated selectively for specific target cell types and can be used in an individual organism. The objective can be achieved by a compound represented by general formula K-Q-X, which is prepared by binding bioactive substance X, photocleavable protecting group Q, and compound K, which can be an enzyme substrate and is dissociated from Q-X by an enzyme reaction, wherein: Q is a protecting group that is photocleaved by light with a specific wavelength and then dissociated from X, when K is not bound thereto; X is a substance that does not express bioactivity when Q is bound thereto, but expresses bioactivity when Q is dissociated therefrom; and K is dissociated from Q by the above enzyme, so as to form a compound represented by Q-X.Type: ApplicationFiled: February 25, 2014Publication date: September 11, 2014Applicant: Toho UniversityInventors: Toshiaki FURUTA, Masaki SONE, Akinobu SUZUKI, Eri OKA
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Patent number: 8822211Abstract: The invention concerns a device and a method for concentrating pathogenic germs potentially present in blood products or derivatives and for detecting said germs comprising the following steps: (a) subjecting a sample of said blood product to a blood cell aggregating treatment, (b) eliminating the aggregates formed at step (a) by passing the treated sample over a first filter allowing through the contaminating germs but not the cell aggregates, (c) selectively lyzing the residual cells of the filtrate obtained at step (b), (d) recuperating the contaminating germs by passing the lysate of step (c) over a second filter to detect the contaminating germs possibly trapped.Type: GrantFiled: June 11, 2013Date of Patent: September 2, 2014Assignee: Becton Dickinson Infusion Therapy Systems Inc.Inventors: Jean-Pierre Hermet, Isabelle Besson-Faure, Sebastien Ribault, Yann Godfrin, Anne Monnot des Angles
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Publication number: 20140242662Abstract: A method of growing cells on a droplet actuator is provided. The method may include providing a droplet actuator including a cell culture droplet including a cell culture medium and one or more cells; and maintaining the droplet at a temperature suitable for causing the cells to grow in the cell culture medium on the droplet actuator. Related methods, droplet actuators, and systems are also provided.Type: ApplicationFiled: March 26, 2014Publication date: August 28, 2014Applicant: Advanced Liquid LogicInventors: Michael G. Pollack, Vamsee K. Pamula, Allen E. Eckhardt
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Patent number: 8816234Abstract: A method for sorting particles in a fluid. The method involves generating an optical landscape using an acousto-optic device. Preferably, the optical landscape is arranged to sort particles based on size or refractive index or shape.Type: GrantFiled: September 20, 2007Date of Patent: August 26, 2014Inventors: Michael MacDonald, Kishan Dholakia, Daniel Rhodes, Graham Milne
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Patent number: 8815231Abstract: Systems and methods generally useful in medicine, cellular biology, nanotechnology, and cell culturing are discussed. In particular, at least in some embodiments, systems and methods for magnetic guidance and patterning of cells and materials are discussed. Some specific applications of these systems and methods may include levitated culturing of cells away from a surface, making and manipulating patterns of levitated cells, and patterning culturing of cells on a surface. Specifically, a method of culturing cells is presented. The method may comprise providing a plurality of cells, providing a magnetic field, and levitating at least some of the plurality of cells in the magnetic field, wherein the plurality of cells comprise magnetic nanoparticles. The method may also comprise maintaining the levitation for a time sufficient to permit cell growth to form an assembly.Type: GrantFiled: March 24, 2011Date of Patent: August 26, 2014Assignees: William Marsh Rice University, Board of Regents, The University of Texas SystemInventors: Glauco R. Souza, Renata Pasqualini, Wadih Arap, Thomas Charles Killian, Robert M. Raphael, Daniel Joshua Stark