Enzyme Or Microbial Cell Is Entrapped Within The Carrier (e.g., Gel, Hollow Fibre) Patents (Class 435/182)
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Publication number: 20130004464Abstract: The present invention relates to the identification, isolation, expansion and characterization of a specific type of adult stem cell. These adult stem cells are characterised in that they naturally express many of the markers of totipotency, which have hitherto generally been limited to embryonic cell populations. The cells of the invention display an unprecedented capacity for multipotency; they are able to differentiate into cell types of mesodermal, endodermal and ectodermal origin. These adult stem cells may be used as therapeutic agents including, without limitation, for the regeneration of tissue, particularly for regeneration of damaged cardiac tissue, such as myocardium.Type: ApplicationFiled: April 13, 2012Publication date: January 3, 2013Inventor: Bernardo Nadal-Ginard
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Publication number: 20130005016Abstract: The invention relates to an amphiphilic polymer comprising at least 75% of amphiphilic monomers of formula (I), the average molar mass of the polymer being between 800 and 100 000, and also water-soluble complexes between the polymer according to the invention and a hydrophobic or amphiphilic compound, in particular a membrane protein, a concentrated aqueous solution of one or more such complex(es), a product comprising one or more such complex(es) attached to a substrate by means of the polymer according to the invention, and various uses of these products.Type: ApplicationFiled: November 16, 2010Publication date: January 3, 2013Applicant: Centre National de la Recherche Scientifique (CNRSInventors: Bernard Pucci, Jean-Luc Popot, Sharma Kshatrapati Shivaji, Fabrice Giusti, Paola Bazzacco, Gregory Durand
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Publication number: 20130005013Abstract: Disclosed are compositions of matter comprising at least one anaerobic degrading microorganism, particulate active carbon and a polymeric solid support wherein the anaerobic degrading microorganism and particulate active carbon are entrapped in the polymeric support and processes for preparing the same. The disclosed compositions of matter are used in processes of anaerobic degradation of waste materials resulting in high yields of biogas production.Type: ApplicationFiled: March 17, 2011Publication date: January 3, 2013Applicant: AgROBICS, LTD.Inventors: Isam Sabbah, Nedal Massalha, Ahlam Saliba
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Patent number: 8343742Abstract: This invention relates to a method for the encapsulation of cells in biologic compatible three dimensional scaffolds and the use of such cells encapsulated in a scaffold. The cells are embedded in a charged polymer that is complex coacervating with an oppositely charged polymer within biologic compatible scaffolds. The polymer complex embedding the cells is forming an ultra thin membrane on the surface of the three dimensional scaffold.Type: GrantFiled: January 25, 2010Date of Patent: January 1, 2013Assignee: Agency for Science, Technology and ResearchInventors: Hanry Yu, Yi Chin Toh
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Publication number: 20120309053Abstract: A 3-dimensional porous polymeric structure comprising a porous polymer structure optionally with particles within the pores of the polymer and wherein the pores have a narrow pore-size distribution. The structure may be made by closely packing particles in a zone to provide a 3-dimensional array, contacting a polymerisable monomer with the array such that the composition fills interstitial spaces between the particles and effecting polymerisation of the monomer whereby a polymer structure is formed around the particles and optionally removing the particles from the structure. The 3-dimensional porous structure may be used in solid phase synthesis, immobilisation, cell culturing and preparation of a stationary phase for chromatographic separation, as an absorbent, an insulating material or in tissue regeneration.Type: ApplicationFiled: September 16, 2010Publication date: December 6, 2012Applicant: SPHERITECH LTDInventor: Donald A. Wellings
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Patent number: 8323956Abstract: Enzymatic biosensors and methods of producing distal tips for biosensor transducers for use in detecting one or more analytes selected from organic compounds susceptible to dehalogenation, organic compounds susceptible to oxygenation, organic compounds susceptible to deamination, organosulfate compounds susceptible to hydrolysis, and organophosphate compounds susceptible to hydrolysis are disclosed herein, as well as biosensor arrays, methods of detecting and quantifying analytes within a mixture, and devices and methods for delivering reagents to enzymes disposed within the distal tip of a biosensor.Type: GrantFiled: January 22, 2009Date of Patent: December 4, 2012Assignee: Colorado State University Research FoundationInventors: Kenneth F. Reardon, Lawrence Philip Wackett
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Publication number: 20120301942Abstract: A chip useful for treating cells and the like which has a mechanism and a structure wherein the size of a hole pattern is arbitrarily changed so that cells can easily move in and get out from the hole in scattering or collecting cells but can hardly get out from the hole during washing or antigen-stimulation. The chip comprises a crosslinked product of a temperature-responsive polymer as a constituting member and being provided with a film having a hole pattern on the surface of a baseboard. A method of producing the chip comprises applying a composition containing a crosslinkable temperature-responsive polymer on the surface of a baseboard to thereby form a coating film, crosslinking the coating film to thereby form the crosslinked product as described above and then forming a hole pattern on the coating film of the crosslinked product.Type: ApplicationFiled: August 8, 2012Publication date: November 29, 2012Applicants: TOYAMA PREFECTURE, NISSAN CHEMICAL INDUSTRIES, LTD.Inventors: Eiichi TAMIYA, Yoshiyuki YOKOYAMA, Satoshi FUJIKI, Katsumi TANINO, Atsushi MURAGUCHI, Hiroyuki KISHI, Yoshiharu TOKIMITSU, Shohei YAMAMURA
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Patent number: 8293510Abstract: The present invention is directed to a hydrogel network comprised of a physically cross-linked polymer and a chemically cross-linked polymer or physically entangled copolymer containing living cells, such as chondrocytes, encapsulated therein. In a preferred aspect, the physically cross-linked polymer is selected from the group consisting of thermally gelling polysaccharides and proteins, such as agarose or gelatin, and the chemically cross-linked or physically entangled polymer is synthesized from a water-soluble vinyl monomer, either as a homopolymer or copolymer, such as polyethylene glycol diacrylate (“PEG-DA”) and 2-hydroxyethyl methacrylate (“HEMA”).Type: GrantFiled: November 16, 2007Date of Patent: October 23, 2012Assignee: University of KansasInventors: Michael Detamore, Stevin H. Gehrke
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Patent number: 8283154Abstract: The present invention provides a stable complex microbial system, which simultaneously decomposes a plurality of organic contaminants even under a polluted environment with these contaminants and permits more effective decomposition of persistent organic contaminants such as PCNB and simazine. A support for holding a complexed accumulation of degrading bacteria, which contains a porous material provided as a support on which a degrading bacterium A capable of degrading at least one organic contaminant and a degrading bacterium B capable of degrading another organic contaminant are accumulated, is produced. The degrading bacterium A may be a PCNB-degrading bacterium, particularly a degrading bacterium containing a degrading bacterium having part or all of mycological characteristics of Nocardioides sp. PD653 and the degrading bacterium B may be a degrading bacterium containing a degrading bacterium having part or all of mycological characteristics of ?-Proteobacteria CDB21.Type: GrantFiled: September 16, 2009Date of Patent: October 9, 2012Assignees: National Institute for Agro-Environmental Sciences Independent Administrative Institute, Kowa Co., Ltd.Inventors: Kazuhiro Takagi, Naoki Harada, Yuuichi Yoshioka
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Publication number: 20120252090Abstract: A process for stabilizing a bioactive composition includes forming hydrogel matrix pores around protein molecules and reducing a water content within the hydrogel matrix pores while keeping the protein molecules biologically active.Type: ApplicationFiled: June 18, 2012Publication date: October 4, 2012Applicants: Regents of the University of Minnesota, Toyota Motor Corporation, Toyota Motor Engineering & Manufacturing North America, Inc.Inventors: Ping Wang, Songtao Wu, Hongfei Jia, Masahiko Ishii, Xiaodong Tong, Minjuan Zhang
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Patent number: 8252607Abstract: The disclosure provides a simple and effective way of synthesizing robust organic-inorganic hybrid gels and ultra-thin films using vaporization of a gel precursor. The gels are synthesized at relatively low temperature allowing the activity of the immobilized species to be maintained. The disclosure provides robust, synthetic, selective, active and/or passive transport systems in the form of functional biologically active species and mechanisms for forming them. These systems allow selective and passive or active transport of ionic, molecular and biological species through the incorporation of functional biological molecules and biomolecular assemblies in a rigid matrix.Type: GrantFiled: June 6, 2008Date of Patent: August 28, 2012Inventors: Gautam Gupta, Gabriel Lopez, Plamen Atanassov
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Patent number: 8241890Abstract: A method and device are disclosed for industrial production of immobilized biocatalysts such as enzymes or microorganisms. In the method, a polyvinyl alcohol gel is produced containing the biocatalysts, and the gel is shaped in a stream of drying air. The device includes a continuous conveyor belt. A casting mechanism is positioned above the belt for applying a mixture containing a biocatalyst to the belt. Positioned for the belt to pass through following the casting mechanism, is a drying channel, a reswelling tank, a wiping and collecting device, a rinse box, and a drying channel.Type: GrantFiled: March 6, 2007Date of Patent: August 14, 2012Assignee: Lentikat's, A.S.Inventor: Radek Stloukal
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Publication number: 20120202261Abstract: This disclosure relates generally to methods, apparatuses, and cellular compositions and cellular products that use a unique trophoblast-containing 3D matrix as a compartmental chamber for growing embryoid bodies that can be induced to differentiate into organ-specific cell types.Type: ApplicationFiled: February 3, 2011Publication date: August 9, 2012Inventor: Yansong Gu
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Patent number: 8231889Abstract: Polyhydroxyalkanoates (PHAs) from which pyrogen has been removed are provided for use in numerous biomedical applications. PHAs which have been chemically modified to enhance physical and/or chemical properties, for targeting or to modify biodegradability or clearance by the reticuloendothelial system (RES), are described. Methods for depyrogenating PHA polymers prepared by bacterial fermentation processes are also provided, wherein pyrogens are removed from the polymers without adversely impacting the polymers' inherent chemical structures and physical properties. PHAs with advantageous processing characteristics, including low melting points and/or solubility in non-toxic solvents, are also described. PHAs are provided which are suitable for use in in vivo applications such as in tissue coatings, stents, sutures, tubing, bone and other prostheses, bone or tissue cements, tissue regeneration devices, wound dressings, drug delivery, and for diagnostic and prophylactic uses.Type: GrantFiled: February 4, 2011Date of Patent: July 31, 2012Assignee: Metabolix, Inc.Inventors: Simon F. Williams, David P. Martin, Tillman Gerngross, Daniel M. Horowitz
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Patent number: 8227238Abstract: A large amount of entrapping immobilization pellets with highly stable quality are produced inexpensively by high-speed treatment. There is provided a process for producing entrapping immobilization pellets in which microorganisms are entrapped and immobilized in an immobilizing agent, the process comprising polymerizing a mixture containing the microorganisms and a solution of the immobilizing agent in a forming frame into a gel to prepare a pellet block.Type: GrantFiled: March 13, 2009Date of Patent: July 24, 2012Assignee: Hitachi Plant Technologies, Ltd.Inventors: Naoki Abe, Koutarou Aoyama, Tatsuo Sumino, Hiroyoshi Emori, Takayuki Kouno
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Patent number: 8222015Abstract: A bioactive composition includes a hydrogel matrix. At least one protein is immobilized in the hydrogel matrix. The digestive protein has a half-life at least 1000 times longer than the half-life of a free digestive protein counterpart.Type: GrantFiled: May 9, 2008Date of Patent: July 17, 2012Assignees: Toyota Motor Engineering & Manufacturing North America, Inc., Toyota Motor Corporation, Regents of the University of MinnesotaInventors: Ping Wang, Songtao Wu, Hongfei Jia, Masahiko Ishii, Xiaodong Tong, Minjuan Zhang
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Patent number: 8202701Abstract: Compositions and methods for producing encapsulated cells having an average diameter of less than about 200 ?m are provided. Methods for using the disclosed encapsulated cells are also provided.Type: GrantFiled: October 7, 2005Date of Patent: June 19, 2012Assignee: Georgia Tech Research CorporationInventors: Barbara Dale Boyan, Ramsey Christian Kinney, Zvi Schwartz
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Patent number: 8202833Abstract: The present invention provides method and materials for forming a polymeric matrix having improved biocompatible properties. A polymerization accelerator is provided that includes an N-vinyl group and a biocompatible functional group. The polymerization accelerator is particularly useful for the polymerization of macromers, which can be used to form biocompatible polymeric coatings on the surface of biological materials, such as cells and tissue.Type: GrantFiled: November 26, 2003Date of Patent: June 19, 2012Assignee: Surmodics, Inc.Inventors: Dale G. Swan, Stephen J. Chudzik, Ronald F. Ofstead
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Patent number: 8198062Abstract: Provided are hydrolases, including lipases, saturases, palmitases and/or stearatases, and polynucleotides encoding them, and methods of making and using these polynucleotides and polypeptides. Further provided are polypeptides, e.g., enzymes, having a hydrolase activity, e.g., lipases, saturases, palmitases and/or stearatases and methods for preparing low saturate or low trans fat oils, such as low saturate or low trans fat animal or vegetable oils, e.g., soy or canola oils.Type: GrantFiled: August 29, 2008Date of Patent: June 12, 2012Assignee: DSM IP Assets B.V.Inventors: Timothy Hitchman, Christopher L. G. Dayton, Katie A. Kline, Jonathan Lyon, Mark Wall, Nelson R. Barton
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Patent number: 8192970Abstract: The method for treating microcystin-containing water which detoxifies microcystin in the microcystin-containing water, the method comprises the step of: bringing the microcystin-containing water into contact with a Sphingomonas bacterium to degrade biologically the microcystin in the microcystin-containing water, wherein: the Sphingomonas bacterium is a strain FERM P-19480 which is deposited as strain MDB1 with International Patent Organism Depositary, National Institute of Advanced Industrial Science and Technology.Type: GrantFiled: December 31, 2007Date of Patent: June 5, 2012Assignee: Hitachi Plant Technologies, Ltd.Inventors: Tatsuo Sumino, Takako Ogasawara, Ho-Dong Park
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Patent number: 8178332Abstract: The present invention generally relates to the removal of carbon dioxide from a gas stream, particularly a flue gas, hydrogen gas from a reformer, natural gas, or gas from a cement kiln. Immobilized enzymes for use in carbon capture and other systems are also disclosed.Type: GrantFiled: August 15, 2011Date of Patent: May 15, 2012Assignee: Akermin, Inc.Inventors: Wayne L. Gellett, Tracy L. Bucholz, Richard T. Zvosec, Joshua Schumacher, Robert A. Clayton, Robert P. Shirtum
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Publication number: 20120107900Abstract: The present invention provides electrospun polymer fibers comprising bacteria-containing hydrogel particles. Bacteria-containing hydrogel particles are produced by crosslinking water-soluble polymers to form hydrogels and mixing them with a bacteria suspension. The crosslinking is suitable to be carried out either chemically before the addition of the bacteria suspension or physically before or after this addition. Subsequently, these hydrogel particles are electrospun together with an electrospinnable polymer solution to form fibers or fibre non-wovens. The bacteria which are located in these hydrogel particles or in the electrospun polymer fibers comprising these particles, respectively, are capable of surviving for a long period (several months) without the supply of water or cell-culture media and are simultaneously protected against the effect of solvents, for example alcohols, acetone, chlorinated hydrocarbons, ethers and toluene, which would otherwise kill said bacteria.Type: ApplicationFiled: December 18, 2009Publication date: May 3, 2012Applicant: Philipps-Universitat MarburgInventors: Andreas Greiner, Seema Agarwal, Marco Gensheimer
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Patent number: 8143043Abstract: Positively-charged initiator polymers having a polymerization initiator group and a cationic portion are provided. The initiator polymers can be used with a polymerizable material for the formation of a polymeric matrix on a surface. The initiator polymers are particularly useful for cell encapsulation using macromers.Type: GrantFiled: April 2, 2009Date of Patent: March 27, 2012Assignee: Surmodics, Inc.Inventors: Stephen J. Chudzik, Ronald F. Ofstead, Dale G. Swan
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Patent number: 8143042Abstract: The present inventions in various aspects provide elastic biodegradable polymers. In various embodiments, the polymers are formed by the reaction of a multifunctional alcohol or ether and a difunctional or higher order acid to form a pre-polymer, which is cross-linked to form the elastic biodegradable polymer. In preferred embodiments, the cross-linking is performed by functionalization of one or more OR groups on the pre-polymer backbone with vinyl, followed by photopolymerization to form the elastic biodegradable polymer composition or material. Preferably, acrylate is used to add one or more vinyls to the backbone of the pre-polymer to form an acrylated pre-polymer. In various embodiments, acrylated pre-polymers are co-polymerized with one or more acrylated co-polymers.Type: GrantFiled: January 12, 2007Date of Patent: March 27, 2012Assignee: Massachusetts Institute of TechnologyInventors: Christopher J. Bettinger, Joost P. Bruggeman, Lino Da Silva Ferreira, Jeffrey M. Karp, Robert S. Langer, Christiaan Nijst, Andreas Zumbuehl, Jason Burdick, Sonia J. Kim
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Patent number: 8142769Abstract: The invention relates to developments in the treatment of diabetes in mammals. Particularly it relates to a method of preparing a xenotransplantable porcine islet preparation capable upon xenotransplantation of producing porcine insulin in an appropriate recipient mammal, the method including or comprising the steps of: (I) harvesting the pancreas of piglets at or near full term gestation, and (ii) extracting pancreatic ? islet cells from the harvested pancreas wherein the islets (at least at some stage in the performance of the method) are exposed to nicotinamide. Further, the invention relates to a method of encapsulation of a xenotransplantable porcine islet preparation, and transplantation of such a preparation, or a capsule containing such a preparation, into an appropriate recipient mammal.Type: GrantFiled: October 31, 2007Date of Patent: March 27, 2012Assignee: Diabcell Pty LtdInventors: Robert Bartlett Elliott, Riccardo Calafiore, Gusseppe Basta
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Patent number: 8137972Abstract: Scaffolds for tissue engineering prepared from biocompatible, biodegradable polymer-based, lighter than or light as water microcarriers and designed for cell culturing in vitro in a rotating bioreactor are provided. Methods for preparation and use of these scaffolds as tissue engineering devices are also provided.Type: GrantFiled: May 30, 2007Date of Patent: March 20, 2012Assignees: Drexel University, The Wistar Institute, The Trustees Of The University of PennsylvaniaInventors: Cato T. Laurencin, Solomon R. Pollack, Elliot Levine, Edward Botchwey, Helen H. Lu, Mohammed Yusuf Khan
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Publication number: 20120064026Abstract: A method for preventing and controlling the formation of biofouling on an object that is immersed or partially immersed in a water environment in which there is one or more organic or inorganic compounds capable of releasing gas, comprising the step of applying to the surface of the object a composition comprising a polymeric resin and one or more enzymes or a paint or a coating and one or more enzymes, wherein the one or more enzymes are adapted to catalyze a reaction of the one or more compounds that leads to the formation of gas.Type: ApplicationFiled: May 19, 2010Publication date: March 15, 2012Inventors: Alejandro Hochkoeppler, Lucio Panizza, Elena Roda, Alessandra Stefan
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Patent number: 8110385Abstract: A linear biodevice is provided with fibers that have a curved portion in its cross section approximately perpendicular to the longitudinal direction and approximately spherical adhesive cells that adhere to the periphery of the fiber. A membrane-like biodevice is provided with a sheet that has an opening and approximately spherical adhesive cells that adhere to the opening. A curved portion is present in the cross section on an inner edge of the opening. A bioreactor is provided with a membrane-like biodevice in which spherical adhesive cells grow densely so as to close the openings of the sheet.Type: GrantFiled: June 30, 2005Date of Patent: February 7, 2012Assignee: Japan Agency for Marine-Earth Science and TechnologyInventors: Satoshi Konishi, Tetsuya Miwa
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Patent number: 8110213Abstract: Polymeric materials are used to make a pliable, non-toxic, injectable porous template for vascular ingrowth. The pore size, usually between approximately 100 and 300 microns, allows vascular and connective tissue ingrowth throughout approximately 10 to 90% of the matrix following implantation, and the injection of cells uniformly throughout the implanted matrix without damage to the cells or patient. The introduced cells attach to the connective tissue within the matrix and are fed by the blood vessels. The preferred material for forming the matrix or support structure is a biocompatible synthetic polymer which degrades in a controlled manner by hydrolysis into harmless metabolites, for example, polyglycolic acid, polylactic acid, polyorthoester, polyanhydride, or copolymers thereof. The rate of tissue ingrowth increases as the porosity and/or the pore size of the implanted devices increases.Type: GrantFiled: July 15, 2008Date of Patent: February 7, 2012Assignees: Massachusetts Institute of Technology, Children's Medical Center CorporationInventors: Antonios G. Mikos, Robert S. Langer, Joseph P. Vacanti, Linda G. Griffith, Georgios Sarakinos
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Patent number: 8093027Abstract: The present invention concerns a biocomposite useful in artificial tissue replacement, methods for its production, and methods of use. The biocomposite can be implanted into humans or animals as an artificial tissue for treatment of a tissue defect. The biocomposite can be formed or manipulated into a desired shape for implantation.Type: GrantFiled: September 13, 2007Date of Patent: January 10, 2012Assignee: University of South FloridaInventors: Edward Turos, Thomas J. Koob, Kerriann Robyn Greenhalgh
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Patent number: 8080385Abstract: A polymer matrix that may coated on an electrode is created by co-crosslinking (1) an adduct of a polyaniline formed by templated oxidative polymerization on a polymer acid; (2) a water-soluble crosslinker; and (3) a redox enzyme. The polymer matrix may be hydrated, and the absorbed water may make it permeable to, for example, glucose. The polyaniline may be polyaniline itself or a substituted polyaniline; the water-soluble crosslinker may be poly(ethylene glycol) diglycidyl ether, and the redox enzyme may be glucose oxidase. The polymer matrix may be produced by co-crosslinking (1) an adduct of an electrically conductive polymer and a polymer acid; (2) a water-soluble crosslinker; and (3) a redox enzyme in a single step at an about neutral pH, curing by drying. After hydration, the crosslinked polymer matrix may form a 3-dimensional glucose-permeable bioelectrocatalyst, catalyzing the electrooxidation of glucose.Type: GrantFiled: May 2, 2008Date of Patent: December 20, 2011Assignee: Abbott Diabetes Care Inc.Inventors: Adam Heller, Benjamin J. Feldman, Nicolas Mano, Yueh-Lin Loo
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Patent number: 8076113Abstract: A granule with an allergenic component has reduced dust by including antifoam added during the production of the granule. The antifoam may be dispersed throughout the granule or added to one of the components of the granule. The granule with antifoam produces at least 30% less dust than a comparable granule produced according to a process in which no antifoam is added.Type: GrantFiled: April 1, 2002Date of Patent: December 13, 2011Assignee: Danisco US Inc.Inventors: Douglas A. Dale, Thomas S. Green
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Publication number: 20110287509Abstract: The present invention provides a stable and viable biocatalyst with high activity and operational stability and method of immobilization thereof. The immobilization process is based on the principle of entrapment of partially purified enzyme precipitate which is simultaneously aggregated by cross linking agent like glutaraldehyde and further entrapped in a combination of natural polymer like gelatin and synthetic polymer like polyvinyl alcohol with effective gelation under mild conditions of temperature and pH, resulting in a stable biocatalyst. The enzymes immobilized by the above process include Penicillin acylase from rE. coli RE III (pKA18), Novel Penicillin acylase from Achromobacter sp (CCM4834) expressed in rE. coli BL21 (pK1P1) CCM 7394 and rE. coli RE III (pKX1P1).Type: ApplicationFiled: November 14, 2008Publication date: November 24, 2011Applicants: Mikrobiologicky Ustav Avcr V.V.I., Fermenta Biotech LimitedInventors: Anupama Datla, Williams Rajasekar Vyasarayani, Trupti Krishnakant Ashar, Kyslik Pavel, Becka Stanislav
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Patent number: 8030095Abstract: A gel microdrop composition is provided. In certain embodiments, the gel microdrop composition contains a polymer matrix, an effector particle that releases an effector molecule into the polymer matrix, a first reporter particle that emits a first optically detectable signal and a second reporter particle that emits a second optically detectable signal that is distinguishable from the first optically detectable signal, where the effector particle and said first and second reporter particles are encapsulated by the polymer matrix. Methods of screening that employ the gel microdrop composition and methods of making the gel microdrop composition are also disclosed.Type: GrantFiled: March 2, 2009Date of Patent: October 4, 2011Assignee: Crystal Bioscience Inc.Inventor: William Don Harriman
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Patent number: 8029816Abstract: The present invention is directed to medical devices including coatings. The coatings include a topcoat which includes a copolymer comprising a block of an elastin pentapeptide. The topcoat is over a layer of poly(vinyl alcohol) on a hydrophobic coating or over a porous coating comprising pores or depots that include a bioactive agent.Type: GrantFiled: May 10, 2007Date of Patent: October 4, 2011Assignee: Abbott Cardiovascular Systems Inc.Inventors: Syed Faiyaz Ahmed Hossainy, Yiwen Tang, Lothar W. Kleiner
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Patent number: 8030041Abstract: A large amount of entrapping immobilization pellets with highly stable quality are produced inexpensively by high-speed treatment. There is provided a process for producing entrapping immobilization pellets in which microorganisms are entrapped and immobilized in an immobilizing agent, the process comprising polymerizing a mixture containing the microorganisms and a solution of the immobilizing agent in a forming frame into a gel to prepare a pellet block.Type: GrantFiled: June 2, 2006Date of Patent: October 4, 2011Assignee: Hitachi Plant Technologies, Ltd.Inventors: Naoki Abe, Koutarou Aoyama, Tatsuo Sumino, Hiroyoshi Emori, Takayuki Kouno
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Patent number: 8021869Abstract: Cell storage and delivery systems and methods for storing and delivering viable cells to a mammal are disclosed. The cell storage and delivery systems include a biodegradable and/or bioabsorbable fibrous matrix physically associated with viable cells to contain and release the cells at a controlled rate. The biodegradable and/or bioabsorbable matrix can be formed by electrospinning fibers of biodegradable and/or bioabsorbable fiberizable material. The methods include methods for storing viable cells and for delivering viable cells to a mammal using the cell storage and delivery system.Type: GrantFiled: November 20, 2007Date of Patent: September 20, 2011Assignee: The Research Foundation of State University of New YorkInventors: Benjamin Chu, Benjamin S. Hsiao, Michael Hadjiargyrou, Dufei Fang, Xinhua Zong, Kwangsok Kim
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Patent number: 7998714Abstract: The present invention generally relates to the removal of carbon dioxide from a gas stream, particularly a flue gas, hydrogen gas from a reformer, natural gas, or gas from a cement kiln. Immobilized enzymes for use in carbon capture and other systems are also disclosed.Type: GrantFiled: September 29, 2009Date of Patent: August 16, 2011Assignee: Akermin, Inc.Inventors: Wayne L. Gellett, Tracy L. Bucholz, Richard T. Zvosec, Joshua Schumacher, Robert A. Clayton, Robert P. Shirtum
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Publication number: 20110124079Abstract: Sorption reinforced catalytic coating system for the degradation of threat agents including a synzyme coating about a material configured for the degradation of the threat agents, the synzyme coating including bucket-shaped molecules configured for the sorption of the threat agents A binding agent is configured for synzyme immobilization to maximize loading and retention of the synzyme coating on the material.Type: ApplicationFiled: June 17, 2010Publication date: May 26, 2011Inventors: Yongwoo Lee, Tomasz Modzelewski, John P. Puglia, Steven E. Weiss, Robert A. Reinstein
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Publication number: 20110117623Abstract: The present invention provides a thermoresponsive nanoparticle useful for the stabilization of enzymes in environments having a temperature greater than thirty degrees Centigrade. The thermoresponsive nanoparticle has (a) a functionalized enzyme conjugate having one or more enzymes or biological catalysts, the enzymes or biological catalysts are modified with palmitic acid N-hydroxysuccinimide ester and acryclic acid N-hydroxysuccinimide ester, and (b) a thermally responsive polymer, wherein the functionalized enzyme conjugate is encapsulated within the thermally responsive polymer. A nanocatalyst is provided that has one or more proteins. The proteins are covalently immobilized and encapsulated within a thermally responsive polymer shell. The proteins are one or more enzymes or biological catalysts. A method for protecting the proteins is also set forth.Type: ApplicationFiled: November 11, 2010Publication date: May 19, 2011Inventors: Jeremy P. Walker, Anna M. Leech
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Patent number: 7943353Abstract: A multi-layered microcapsule has an inner extracellular matrix and an outer shell. The inner extracellular matrix includes a first inner layer of biopolymer and a second intermediate layer of polymer that provides partial immune-protection and holds the first layer in place. The outer shell can form an exoskeleton to provide mechanical stability. Each of the individual layers can be varied to optimize mechanical stability, cell function, and immuno-protection.Type: GrantFiled: July 23, 2009Date of Patent: May 17, 2011Assignees: Agency for Science, Technology and Research, The National University of SingaporeInventors: Hanry Yu, Kam W Leong, Ser-Mien Chia, Andrew C. A. Wan
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Patent number: 7931894Abstract: It is intended to provide a method of three-dimensionally culturing normal joint chondrocytes; the production and supply of chondrocytes; and a transplantation material to be used in an injured site in a joint tissue.Type: GrantFiled: June 1, 2005Date of Patent: April 26, 2011Assignee: Otsuka Pharmaceutical Co., Ltd.Inventors: Shusa Ohshika, Keiichi Takagaki, Yoko Takagaki, legal representative, Yasuyuki Ishibashi, Satoshi Toh
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Patent number: 7927588Abstract: A skin dressing comprises a first dressing component (16) carrying oxidoreductase enzyme in dried condition; and a second dressing component (18) carrying a source of water, such that when the first and second dressing components are placed in fluid communication with each other, water migrates from the second component towards the first comportent and acts to hydrate enzyme carried by the first component, at least at the surface of the first component. The dressing components are kept separate before use, e.g. being sealed in separate sterile, water-impervious packages such as laminated aluminum foil pouches. In use of the dressing, the second dressing component is located on the skin of a human or animal, e.g. over a wound to be treated or on a region of skin to be treated for cosmetic or therapeutic purposes such as for treatment of acne or other skin conditions. The first dressing component is placed on top of the second component in fluid communication therewith.Type: GrantFiled: June 4, 2004Date of Patent: April 19, 2011Assignee: Archimed LLPInventors: Paul James Davis, Andrew John Austin
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Patent number: 7927827Abstract: A system for the detection of ligands comprising at least one receptor and an amplification mechanism coupled to the receptor wherein an amplified signal is produced as a result of receptor binding a ligand. Examples of suitable amplification mechanisms include antibody-embedded liquid crystalline materials; use of alpha-2-macroglobulin to encage an enzyme, whereby the enzyme is separated from its substrate by an receptor; and a receptor engineered to inhibit the active of site of an enzyme only in the absence of a ligand. Also provided are methods for the automatic detection of ligands.Type: GrantFiled: May 21, 2007Date of Patent: April 19, 2011Assignee: Kent State UniversityInventors: Gary D. Niehaus, Christopher J. Woolverton, Kathleen J. Doane, Steven P. Schmidt, Steven A. Signs
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Patent number: 7919293Abstract: The invention discloses the nanoparticles composed of chitosan, poly-glutamic acid, and at least one protein drug or bioactive agent characterized with a positive surface charge and their enhanced permeability for paracellular protein drug and bioactive agent delivery.Type: GrantFiled: March 6, 2009Date of Patent: April 5, 2011Assignees: GP Medical, Inc., National Tsing Hua UniversityInventors: Hsing-Wen Sung, Mei-Chin Chen, Yu-Hsin Lin, Hsiang-Fa Liang, Hosheng Tu
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Patent number: 7906135Abstract: Polyhydroxyalkanoates (PHAs) from which pyrogen has been removed are provided for use in numerous biomedical applications. PHAs which have been chemically modified to enhance physical and/or chemical properties, for targeting or to modify biodegradability or clearance by the reticuloendothelial system (RES), are described. Methods for depyrogenating PHA polymers prepared by bacterial fermentation processes are also provided, wherein pyrogens are removed from the polymers without adversely impacting the polymers' inherent chemical structures and physical properties. PHAs with advantageous processing characteristics, including low melting points and/or solubility in non-toxic solvents, are also described. PHAs are provided which are suitable for use in vivo applications such as in tissue coatings, stents, sutures, tubing, bone and other prostheses, bone or tissue cements, tissue regeneration devices, wound dressings, drug delivery, and for diagnostic and prophylactic uses.Type: GrantFiled: May 15, 2007Date of Patent: March 15, 2011Assignee: Metabolix, Inc.Inventors: Simon F. Williams, David P. Martin, Tillman Gerngross, Daniel M. Horowitz
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Patent number: 7897042Abstract: Entrapping immobilization pellets for purifying breeding water in an aquarium to breed aquatic animals, wherein the entrapping immobilization pellets have a phosphorus content of 0.05 mass % or less.Type: GrantFiled: March 23, 2009Date of Patent: March 1, 2011Assignee: Hitachi Plant Technologies, Ltd.Inventors: Shinichi Yoshikawa, Naoki Abe, Kazuichi Isaka
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Patent number: 7871638Abstract: This invention relates to a scaffold consisting of a biodegradable polymeric material with a composition gradient of calcium phosphate that is capable of effectively regenerating the hard/soft tissue interface and an implant for hard/soft tissue filling with the utilization of such scaffold.Type: GrantFiled: May 2, 2003Date of Patent: January 18, 2011Assignees: Japan Science and Technology Agency, National Institute for Materials ScienceInventors: Junzo Tanaka, Tetsushi Taguchi
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Patent number: 7851189Abstract: A tissue engineering composition adapted for application to an interior surface of a body lumen of a patient. The composition comprises (a) a carrier medium that is adapted to flow and to stably adhere the composition to the body lumen and (b) microcapsules, which are dispersed within the carrier medium and which contain one or more living cells encapsulated within a coating that includes a biodegradable polymer. The composition promotes growth of the cells on the lumen surface subsequent to application of the composition to the lumen.Type: GrantFiled: March 7, 2005Date of Patent: December 14, 2010Assignee: Boston Scientific Scimed, Inc.Inventors: Toby M. Freyman, Joseph A. Levendusky
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Patent number: 7842185Abstract: Entrapping immobilization pellets in which microorganisms are entrapped and immobilized, the entrapping immobilization pellets comprising entrapped and immobilized purified water sludge containing nitrifying bacteria.Type: GrantFiled: March 20, 2007Date of Patent: November 30, 2010Assignee: Hitachi Plant Technologies, Ltd.Inventors: Naoki Abe, Koutarou Aoyama, Tatsuo Sumino