Oxidoreductase (1. ) (e.g., Luciferase) Patents (Class 435/189)
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Publication number: 20110197294Abstract: The invention provides methods and compositions for treating, ameliorating or preventing diseases or conditions caused by or aggravated by lost and/or impaired mitochondrial Complex I function, including treating, ameliorating or preventing an ischemia and/or reperfusion injury, Parkinson's disease, myopathic diseases, cardiolipin deficiency, neurodegenerative diseases, aging, diabetes, obesity, sepsis and other conditions in which mitochondrial Complex I function is lost and/or impaired.Type: ApplicationFiled: June 4, 2009Publication date: August 11, 2011Inventors: Roberta A. Gottlieb, Cynthia Perry
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Patent number: 7994396Abstract: Transgenic plants, and a method for making the same, wherein genes encoding the enzyme luciferase and its corresponding substrate luciferin are incorporated into a native plant genome. Once transformed into plant cells, these genes may be regulated such that under certain endogenous or exogenous conditions, their expression in the mature plant results in bioluminescence. Different luciferin/luciferase complexes and/or mechanisms of regulation may be utilized for these transgenic plants, depending on a variety of factors such as plant species and the circumstances under which a bioluminescent reaction is desired. Phototransformation may be utilized to vary the wavelength of light emitted from the mature plant.Type: GrantFiled: February 11, 2010Date of Patent: August 9, 2011Inventor: Bruce Eric Hudkins
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Patent number: 7993894Abstract: Nucleic acid molecules from nematodes encoding fatty acid desaturase polypeptides are described. Fatty acid desaturase-like polypeptide sequences are also provided, as are vectors, host cells, and recombinant methods for production of fatty acid desaturase-like nucleotides and polypeptides. Also described are screening methods for identifying inhibitors and/or activators of fatty acid desaturase-like polypeptides, as well as methods for antibody production.Type: GrantFiled: January 15, 2009Date of Patent: August 9, 2011Assignee: Divergence, Inc.Inventors: Andrew P. Kloek, Deryck J. Williams, Merry B. McLaird, John D. Bradley, Jennifer A. DaVila-Aponte, Siqun Xu, Anita M. Frevert
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Publication number: 20110189717Abstract: The invention relates to recombinant expression of a taxadiene synthase enzyme and a geranylgeranyl diphosphate synthase (GGPPS) enzyme in cells and the production of terpenoids.Type: ApplicationFiled: November 10, 2010Publication date: August 4, 2011Applicant: Massachusetts Institute of TechnologyInventors: Parayil K. Ajikumar, Gregory Stephanopoulos, Too Heng Phon
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Publication number: 20110189686Abstract: An in vitro or in vivo method for screening for candidate compounds for the preventive or curative treatment of acne, of seborrhoeic dermatitis or of skin disorders associated with hyperseborrhoea, includes determining the ability of a compound to modulate the expression or the activity of the CYP2B15 and/or glycerol-3-phosphate dehydrogenase 1 (GPD1) proteins.Type: ApplicationFiled: May 7, 2009Publication date: August 4, 2011Applicant: Galderma Research & DevelopmentInventors: Michel Rivier, Isabelle Carlavan, Jérôme Aubert
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Publication number: 20110191903Abstract: Methods for identifying one or more amino acid substitutions in an oxalate oxidase (OXOX) variant polypeptide that confer maintained or increased OXOX activity are described herein. Methods and compositions for increasing a plant's resistance to a pathogen using the modified OXOX variant polypeptides are provided. Transformed plants, plant cell, tissues, seed, and expression vectors are also provided.Type: ApplicationFiled: December 30, 2010Publication date: August 4, 2011Applicant: Pioneer Hi-Bred International, Inc.Inventors: James Joseph English, Xu Hu, Azalea Sukfun Ong, Daniel J. Thorpe, Gusui Wu
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Publication number: 20110189755Abstract: In a method for producing an additive for the enzymatic degradation of mycotoxins, in particular fumonisins, it is provided that at least one nucleic acid sequence of genes corresponding to sequences ID Nos. 1, 2, 4, 6, 8, 10, 12, 14, 16, 18, 20, 22 and 24 is provided, the at least one nucleic acid sequence is expressed in prokaryotic or eukaryotic host cells, and at least one thus prepared enzyme corresponding to sequences ID Nos. 3, 5, 7, 9, 11, 13, 15, 17, 19, 21, 23 and 25, or at least one complete recombinant host organism optionally along with a cosubstrate, are used in a vegetable raw material.Type: ApplicationFiled: September 18, 2009Publication date: August 4, 2011Inventors: Wulf-Dieter Moll, Doris Hartinger, Karin Grießler, Eva Maria Binder, Gred Schtzmayr
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Publication number: 20110191875Abstract: The invention provides compositions and methods for producing androstenedione (4-androstenedione), of improved purity and for modulating its production, for example by deletion or inactivation of ksdA, cxgA, cxgB, cxgC, or cxgD. The invention also provides methods and compositions, including nucleic acids that encode enzymes, for producing 1,4-androstadiene-3,17-dione (ADD) and related pathway compounds, including 20-(hydroxymethyl)pregna-4-en-3-one and 20-(hydroxymethyl)pregna-1,4-dien-3-one. The compositions of the invention include nucleic acids, probes, vectors, cells, transgenic plants and seeds, transgenic animals, kits and arrays.Type: ApplicationFiled: November 13, 2008Publication date: August 4, 2011Applicant: Verenium CorporationInventors: David Nunn, Catherine Pujol, Kelly Chatman
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Patent number: 7989193Abstract: The present invention relates a host cell comprising an expression vector comprising a nucleic acid molecule encoding a protein requiring gamma-carboxylation and associated expression control sequences and a nucleic acid molecule encoding a vitamin K epoxido reductase and associated expression control sequences and a nucleic acid molecule encoding a ?-glutamyl carboxylase and associated control sequences. The invention further relates to a method of producing a protein requiring gamma-carboxylation in high yields.Type: GrantFiled: April 10, 2006Date of Patent: August 2, 2011Assignee: MedImmune LimitedInventor: Ann Lövgren
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Patent number: 7989190Abstract: The present invention provides a firefly luciferase for inexpensive, highly accurate and highly sensitive nucleic acid analysis that uses dATP instead of an expensive reagent having low reactivity to DNA polymerase in the manner of dATP?S, a method of analyzing nucleic acid that uses that luciferase, and a kit for analyzing nucleic acid thereof. The present invention relates to a composition for analyzing nucleic acid that contains luciferase for which reactivity to dATP is equal to or less than 1/400 reactivity to ATP, a method of analyzing nucleic acid that comprises the use of that composition, and a kit for analyzing nucleic acid comprising that composition.Type: GrantFiled: November 19, 2008Date of Patent: August 2, 2011Assignee: Kikkoman CorporationInventors: Shigeya Suzuki, Yukako Kodama, Keiko Kurosawa
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Publication number: 20110183865Abstract: The inventors have found a novel fungal lipoxygenase from Manaporthe salvinii and determined its sequence. They have sequenced the gene and cloned it into E. coli and deposited the clone. Oligonucleotides probes based on the sequence information are useful for screening a eukaryotic library to obtain a lipoxygenase. The lipoxygenase is useful in baking and in a detergent.Type: ApplicationFiled: March 31, 2011Publication date: July 28, 2011Applicant: NOVOZYMES A/SInventors: AKIKO SUGIO, SHINOBU TAKAGI
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Publication number: 20110177540Abstract: An isolated recombinant luciferase having luciferase activity. The recombinant luciferase has an amino acid sequence which differs from the wild-type luciferase from Photinus pyralis, Luciola mingrelica, Luciola cruciata, Luciola lateralis, Hotaria parvula, Pyrophorus plagiophthalamus, Lampyris noctiluca, Pyrocoelia miyako or Photinus pennsylvanica. In the sequence of the recombinant luciferase, the amino acid residue corresponding to phenylalanine 295 in Photinus pyralis wild-type luciferase or to leucine 297 in Luciola mingrelica, Luciola cruciata or Luciola lateralis wild-type luciferases, is mutated compared to the corresponding amino acid which appears in the corresponding wild-type luciferase sequence. The recombinant luciferase has increased thermostability compared to the corresponding wild-type luciferase.Type: ApplicationFiled: February 9, 2011Publication date: July 21, 2011Inventors: David J. Squirrell, Melenie J. Murphy, Rachel L. Price, Christopher R. Lowe, Peter J. White, Laurence C. Tisi, James A. H. Murray
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Publication number: 20110177552Abstract: Methods and systems that include a method comprising: providing a system comprising one or more plastid proteins comprising a signal sequence and one or more chloroplast processing enzymes; and allowing at least one of the one or more chloroplast processing enzymes to cleave at least a portion of a signal sequence from at least one of the one or more plastid proteins.Type: ApplicationFiled: January 18, 2011Publication date: July 21, 2011Inventors: Caroline Virginia McNeil, Alyssa Baevich, Seiichi Paul Tillich Matsuda
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Patent number: 7981652Abstract: The present invention relates to modified cytochrome P450 monooxygenases with an altered substrate profile, to nucleic acid sequences coding therefor, to expression constructs and vectors, to recombinant microorganisms which comprise these vectors, and to processes for the microbiological production of terminally or subterminally hydroxylated aliphatic carboxylic acids.Type: GrantFiled: April 13, 2009Date of Patent: July 19, 2011Assignee: BASF AktiengesellschaftInventors: Bernhard Hauer, Juergen Pleiss, Ulrich Schwaneberg, Jutta Schmitt, Markus Fischer, Rolf Schmid, Qing-shan Li
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Patent number: 7981651Abstract: Lactobacillus acidophilus NCFM nucleic acid molecules, polypeptides, fragments and variants thereof are provided in the current invention. In addition, fusion proteins, antigenic peptides, and antibodies are encompassed. The invention also provides recombinant expression vectors containing a nucleic acid molecule of the invention and cells comprising the expression vectors. Methods for producing the polypeptides of the invention and methods for their use are further provided.Type: GrantFiled: October 17, 2008Date of Patent: July 19, 2011Assignee: North Carolina State UniversityInventors: Todd R. Klaenhammer, Eric Altermann, Andrea Azcarate-Peril
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Publication number: 20110171669Abstract: The present invention relates to an isolated nucleic acid and polypeptide sequence that encodes for a luciferase of Luciola italica, as well as mutants thereof. The luciferase proteins of the present invention have been found to have extended bioluminescence emission that is red- or blue-shifted, and are useful as a bioluminescent marker or as an additive to selected materials.Type: ApplicationFiled: October 1, 2010Publication date: July 14, 2011Inventors: Bruce R. Branchini, Tara L. Southworth, Jennifer P. DeAngelis, Aldo Roda, Elisa Michelini
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Publication number: 20110171698Abstract: ?-Amyrin, a precursor in biosynthesis of soyasapogenol B, is biosynthesized by cyclization of 2,3-oxidosqualene which is generated by the mevalonate pathway, and soyasapogenol B is biosynthesized by two hydroxylations of ?-amyrin. However, a gene of 22-hydroxylase involved in the sequence of reactions has not been identified. The present inventors identified a gene encoding the hydroxylase for oleanene triterpenes at C-22, and found that oleanene triterpenes could be hydroxylated at C-22 by co-expressing this gene together with one or more specific genes. Further, the present inventors found that soyasapogenol B could be efficiently produced in large quantities by co-expressing this gene for 22-hydroxylase with a gene for 24-hydroxylase.Type: ApplicationFiled: January 20, 2010Publication date: July 14, 2011Applicant: MEIJI SEIKA KAISHA, LTD.Inventors: Yutaka Ebizuka, Masaaki Shibuya, Eriko Wakita
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Publication number: 20110173724Abstract: The present invention provides a protein having an activity of oxidizing a dammarane-type triterpene, a gene encoding the same, and use of the protein and the gene. The present invention specifically relates to a protein obtainable from a plant belonging to the genus Glychyrrhiza, which has an activity of oxidizing a dammarane-type triterpene, a gene encoding the same, and use of the protein and the gene. The protein is shown in SEQ ID NO:1, 2, or 13, and the gene encoding the same is shown in SEQ ID NO:3, 4, or 14, respectively. Furthermore, a transformant into which the gene is introduced can be produced, by which a triterpene oxidase can be obtained.Type: ApplicationFiled: August 6, 2008Publication date: July 14, 2011Applicant: NATIONAL UNIVERSITY CORPORATION CHIBA UNIVERSITYInventors: Toshiya Muranaka, Hikaru Seki, Kiyoshi Ohyama, Hiroshi Sudo, Satoru Sawai, Kazuki Saito
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Publication number: 20110172107Abstract: A high throughput RNAi-based assay for identify factors involved in maintaining epigenetic silencing is disclosed. The assay measures reactivation of a silent reporter gene in cells, resulting from RNAi-based knockdown in target mRNA. RNAi-based screening of these silent reporter cells has identified known enzymes that place or remove epigenetic marks on histones, as well as non-enzymatic proteins that function in silencing or in transfer of marks during S-phase. In addition, the screen has been used to identify a number of novel gene products involved in epigenetic silencing, which are also disclosed.Type: ApplicationFiled: April 30, 2009Publication date: July 14, 2011Applicant: FOX CHASE CANCER CENTERInventors: Richard A. Katz, Anna Marie Skalka
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Patent number: 7977075Abstract: The subject invention provides materials and methods wherein unique and advantageous combinations of gene mutations are used to direct carbon flow from sugars to a single product. The techniques of the subject invention can be used to obtain products from native pathways as well as from recombinant pathways. In preferred embodiments, the subject invention provides new materials and methods for the efficient production of acetate and pyruvic acid.Type: GrantFiled: September 22, 2008Date of Patent: July 12, 2011Assignee: University of Florida Research Foundation, Inc.Inventors: Thomas B. Causey, Lonnie O'Neal Ingram, Keelnatham Shanmugam, Shengde Zhou
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Patent number: 7977078Abstract: The present disclosure provides engineered ketoreductase enzymes having improved properties as compared to a naturally occurring wild-type ketoreductase enzyme. Also provided are polynucleotides encoding the engineered ketoreductase enzymes, host cells capable of expressing the engineered ketoreductase enzymes, and methods of using the engineered ketoreductase enzymes to synthesize chiral compounds.Type: GrantFiled: August 24, 2008Date of Patent: July 12, 2011Assignee: Codexis, Inc.Inventors: Jack Liang, Stephane J. Jenne, Emily Mundorff, Rama Voladri, James Lalonde, Gjalt Huisman
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Patent number: 7977083Abstract: Recombinant microorganisms are useful for producing xylitol by fermentation of arabinose. The recombinant microorganisms are produced by transformation of host microorganisms with heterologous polynucleotide sequences coding for each of L-xylulose reductase, D-tagatose 3-epimerase, and L-arabinose isomerase, which transformants express the heterologous polynucleotides at a sufficient functional level to be effective to produce xylitol from arabinose. Production of xylitol is effected by contacting these recombinant microorganisms with a substrate comprising arabinose under conditions effective to produce xylitol from arabinose.Type: GrantFiled: July 12, 2007Date of Patent: July 12, 2011Assignee: The United States of America as represented by the Secretary of AgricultureInventors: Yoshikiyo Sakakibara, Badal C. Saha, Paul Taylor
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Publication number: 20110165619Abstract: Proteins having a cofactor can be secreted in an improved manner in a microorganism belonging to the genus Streptomyces provided that the microorganism contains a nucleic acid sequence which is not naturally present in it and which comprises at least the following sequence sections: a) nucleic acid sequence coding for a protein containing a cofactor, and b) a nucleic acid sequence which is at least 20% identical to the sequence given in SEQ ID NO. 1, or at least 20% identical to the sequence given in SEQ ID NO. 3, or a nucleic acid sequence which is structurally homologous to at least one of these sequences, wherein the amino acid sequence encoded by nucleic acid sequence b) functionally cooperates with the amino acid sequence encoded by nucleic acid sequence a) so that at least the amino acid sequence encoded by nucleic acid sequence a) is secreted by the microorganism.Type: ApplicationFiled: November 29, 2010Publication date: July 7, 2011Inventors: JOHANNES BONGAERTS, STEFAN EVERS, KERSTIN FOH, KARL-HEINZ MAURER
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Publication number: 20110162105Abstract: The present invention relates to nucleic acids derived from Drechslera tritici-repentis, Cylindorcarpon herteronema, Diploida natalensis, Stagonospora nodorum, Microdochium nivalae and Periplaneta americana. The invention also relates to the individual coding sequences and to proteins encoded by these sequences in combination with other sequences as well as to a process for converting oleic acid to linoleic acid to Iinoleic acid and the production of arachidonic acid, eicosapentaenoic acid and/or docosahexaenoic acid in a plant.Type: ApplicationFiled: August 25, 2009Publication date: June 30, 2011Applicants: BASF Plant Science GmbH, Bioriginal Food & Science Corp.Inventors: Jörg Bauer, Toralf Senger, Thorsten Zank, Xiao Qiu, Guohai Wu
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Publication number: 20110159529Abstract: The fusion protein comprising (1) a first region comprising the amino acid sequence of SEQ ID NO: 18 and (2) a second region comprising an amino acid sequence for a polypeptide containing at least one cysteine residue for binding to other useful compound via the thiol group can be modified by chemical modification, and thus has a high catalytic ability for a luminescence activity and is highly available for general purposes.Type: ApplicationFiled: December 22, 2010Publication date: June 30, 2011Applicant: CHISSO CORPORATIONInventors: Satoshi INOUYE, Yuiko SAHARA, Junichi SATO
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Publication number: 20110155576Abstract: A homogeneously-structured catalyst/enzyme composite structure formed by electrophoresis deposition (EPD) method. Catalyst and enzyme are simultaneously deposited onto the electrode surface by the EPD method, so as to form a film of catalyst/enzyme composite thereon. The film of catalyst/enzyme composite includes enzyme for catalyzing the biochemical reaction, and catalyst for increasing the rate of the electrochemical reaction, which are homogeneously mixed and forms a stable and three-dimensional structure. Also, this homogeneously-structured catalyst/enzyme composite is applicable as a working electrode of the bioreceptor in a mini-biosensor.Type: ApplicationFiled: September 22, 2010Publication date: June 30, 2011Applicant: National Taiwan University of Science and TechnologyInventors: Bing-Joe Hwang, Min-Hsin Yeh, Shih-Hong Chang, Chung-Chiun Liu, Chao-Shan Chou
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Patent number: 7968325Abstract: Coexpression of a polyhydroxyalkanoic acid synthase and a either a fatty acid:acyl-CoA transferase or an acyl-CoA synthetase in cells enables the biosynthesis of polyester materials. Plasmids, bacteria, materials, and methods for the preparation of polyesters are disclosed.Type: GrantFiled: June 14, 2004Date of Patent: June 28, 2011Assignee: Metabolix, Inc.Inventors: Silke Hein, Brigitte Sohling, Gerhard Gottschalk, Alexander Steinbuchel
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Publication number: 20110154513Abstract: An object of the present invention is to develop a probe for measuring in real time the kinetics of CREB or actin closely related to brain functions such as memory formation in live animals.Type: ApplicationFiled: August 12, 2009Publication date: June 23, 2011Applicant: University of ToyamaInventors: Tetsuya Ishimoto, Hisashi Mori, Hironori Izumi
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Patent number: 7964381Abstract: The present invention relates to genetically modified proteins with uricolytic activity. More specifically, the invention relates to proteins comprising truncated urate oxidases and methods for producing them, including PEGylated proteins comprising truncated urate oxidases.Type: GrantFiled: September 10, 2010Date of Patent: June 21, 2011Assignee: Savient Pharmaceuticals, Inc.Inventors: Jacob Hartman, Simona Mendelovitz
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Patent number: 7960155Abstract: The invention relates to novel cytochrome P450 monooxygenases comprising a modified substrate specificity, to nucleotide sequences which code therefor, to expression constructs and vectors containing these sequences, and to microorganisms transformed therewith. The invention also relates to methods for microbiologically oxidizing different organic substrates, such as methods for producing indigo and indirubin.Type: GrantFiled: July 27, 2000Date of Patent: June 14, 2011Assignee: BASF AktiengesellschaftInventors: Bernhard Hauer, Juergen Pleiss, Ulrich Schwaneberg, Jutta Schmitt, Markus Fischer, Rolf Schmid, Qing-shan Li, Sabine Lutz-Wahl, Daniel Appel
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Patent number: 7960156Abstract: A DNA encoding a glucose dehydrogenase enzyme with high substrate specificity and recombinant vectors and transformants for expression of the DNA are disclosed. By culturing the transformant, the glucose dehydrogenase enzyme can be produced at a low cost. The glucose dehydrogenase enzyme produced is not affected by oxygen dissolved in a measurement sample and, in particular, has superior thermal stability.Type: GrantFiled: April 21, 2010Date of Patent: June 14, 2011Inventor: Koji Sode
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Publication number: 20110138490Abstract: The present invention relates to a polynucleotide from Emiliana huxleyi which codes for a desaturase and which can be employed for the recombinant production of polyunsaturated fatty acids. The invention furthermore relates to vectors, host cells and transgenic nonhuman organisms which comprise the polynucleotide according to the invention, and to the polypeptides encoded by the polynucleotide. The invention furthermore relates to antibodies against the polypeptide according to the invention. Finally, the invention also relates to production methods for the polyunsaturated fatty acids and for oil, lipid and fatty acid compositions and to their use as drugs, cosmetics, foodstuffs, feedstuffs, preferably fish food, or food supplements.Type: ApplicationFiled: April 29, 2009Publication date: June 9, 2011Inventors: Joerg Bauer, Johnathan A. Napier, Olga Sayanova
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Patent number: 7955828Abstract: The invention relates to the use of methionine synthase inhibitors for the treatment of fungal diseases of crops. The invention further relates to methods for treatment of crops against fungal diseases comprising the application of a methionine synthase inhibitor also methods for the identification of novel fungicidal compounds comprising a step for identification of methionine synthase inhibitors.Type: GrantFiled: December 20, 2005Date of Patent: June 7, 2011Assignee: Bayer Cropscience AGInventors: Michel Droux, Marc-Henri Lebrun
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Publication number: 20110131671Abstract: The present invention relates to a polymorphic CYP2C8-polynucleotide. Moreover, the invention relates to genes or vectors comprising the polynucleotides of the invention and to a host cell genetically engineered with the polynucleotide or gene of the invention. Further, the invention relates to methods for producing molecular variant polypeptides or fragments thereof, methods for producing cells capable of expressing a molecular variant polypeptide and to a polypeptide or fragment thereof encoded by the polynucleotide or the gene of the invention or which is obtainable by the method or from the cells produced by the method of the invention. Furthermore, the invention relates to an antibody which binds specifically the polypeptide of the invention. Moreover, the invention relates to a transgenic non-human animal. The invention also relates to a solid support comprising one or a plurality of the above mentioned polynucleotides, genes, vectors, polypeptides, antibodies or host cells.Type: ApplicationFiled: September 10, 2010Publication date: June 2, 2011Inventors: Anja Penger, Reimund Sprenger, Ulrich Brinkmann
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Publication number: 20110131681Abstract: Oxygenic photosynthesis is the major site of production of reactive oxygen species (ROS). Under high temperature stress, increased ROS damage the photosynthetic machinery, membranes and proteins of plants. The present invention is directed to methods for increasing the stress tolerance of plants by expressing PvGrx5 in the plants.Type: ApplicationFiled: July 16, 2009Publication date: June 2, 2011Inventors: Bala Rathinasabapathi, Sabarinath Sundaram
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Publication number: 20110129895Abstract: A method is provided for purifying protein having steps of loading a sample containing proteins into a column containing an ion exchanger in a first direction to allow the ion exchanger to absorb proteins; and passing a salt solution through the column in a second direction opposite to the first direction to elute a target protein from the ion exchanger. The method is proven as capable of improving homogeneity of eluted target protein without combining minute and complicated techniques for protein purification. Therefore, the method is convenient, efficient and economic for purifying proteins.Type: ApplicationFiled: May 25, 2010Publication date: June 2, 2011Applicant: Tamkang UniversityInventors: Ming-Kai Chern, Wei-Jhy Shiah, Jyun-Jie Chen, Tzung-You Tsai, Hsin-Yin Lin, Chien-Wei Liu
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Patent number: 7951210Abstract: The invention relates to catalytic systems which are used to generate colors on a support. The inventive means are characterized in that they comprise one or more deactivated oxidation catalysts. The invention can be used to color organic or inorganic supports.Type: GrantFiled: May 13, 2005Date of Patent: May 31, 2011Assignees: Doublet Luc, DoubletInventors: Daniel Thomas, Catherine Bedel-Cloutour, Sylviane Pulvin-Houde, Laurent Bedouet
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Patent number: 7951997Abstract: Methods for the production of reversibly male-sterile plants by introduction of a polynucleotide encoding a cytokinin oxidase are disclosed along with nucleic acid constructs and transformed cells useful in the production of such plants. Also disclosed is the use of plants containing recombinant nucleic acid sequences in preventing pollination of plants with pollen containing one or more transgenes and in the introduction of economically important traits into elite varieties of plants.Type: GrantFiled: April 16, 2007Date of Patent: May 31, 2011Assignee: Monsanto Technology LLCInventors: Shihshieh Huang, Lyle Dean Crossland, Roy O. Morris, Nordine Cheikh
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Patent number: 7951562Abstract: This invention relates to a multifunctional enzyme having lysyl hydroxylase and glycosyltransferase activity and to the use of the enzyme in glycosylating hydroxylysine residues. In particular this invention relates to a method for producing glycosyltransferase activity, which comprises that a nucleotide sequence encoding lysyl hydroxylase and glycosyltransferase activity is introduced and expressed in a chosen host and the protein product is recovered from the host cell or from the culture medium.Type: GrantFiled: October 31, 2007Date of Patent: May 31, 2011Assignee: Oulun YliopistoInventors: Raili Myllyla, Jari Heikkinen, Maija Risteli, Chunguang Wang
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Publication number: 20110126325Abstract: The present invention relates to enzymes which possess hydroxylase activity, in particular 42 hydroxylase activity, that can be used in methods of synthesizing modified fatty acids.Type: ApplicationFiled: April 24, 2009Publication date: May 26, 2011Inventors: Xue-Rong Zhou, Surinder Pal Singh, Allan Green, Anthony Richard Ashton, Andrew John William Rodgers, Geoffrey John Dumsday, Florian Hans Maximilian Graichen
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Patent number: 7947480Abstract: This invention is directed to nucleic acids which encode the proteins that direct the synthesis of the orthosomycin everninomicin and to use of the nucleic acids and proteins to produce compounds exhibiting antibiotic activity based on the everninomycin structure. The DNA sequence for the gene clusters responsible for encoding everninomicin biosynthetic genes, which provide the machinery for producing everninomicin, are provided. Thus, this invention provides the nucleic acid sequences needed to synthesize novel everninomicin-related compounds based on everninomicin, arising from modifications of the DNA sequence designed to change glycosyl and modified orsellinic acid groups contained in everninomicin. A Micromonospora site-specific integrase gene is also provided, which can be incorporated in a vector for integration into any actinomycete, and, particularly into Monospora.Type: GrantFiled: September 3, 2010Date of Patent: May 24, 2011Assignee: Schering CorporationInventors: Thomas J. Hosted, Tim X. Wang, Ann C. Horan
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Publication number: 20110117624Abstract: The present invention relates to isolated proteases of the RP-II type and variants of RP-II proteases exhibiting improved properties in comparison to the parent RP-II protease, DNA constructs and vectors coding for the expression of said proteases and variants, host cells capable of expressing the proteases and variants from the DNA constructs, as well as a method of producing them by cultivating said host cells. The proteases may advantageously be used as constituents in detergent compositions and additives, optionally in combination with other enzymes such as proteases, lipases, cellulases, amylases, peroxidases or oxidases.Type: ApplicationFiled: January 26, 2011Publication date: May 19, 2011Applicant: Novozymes A/SInventors: Mads Norregaard-Madsen, Peter Rabbek Østergaard, Claus Bo Voge Christensen, Søren Flensted Lassen
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Patent number: 7943338Abstract: The invention relates to methods and materials involved in diagnosing and treating autoimmune conditions. In particular, the invention relates to methods and materials involved in diagnosing arthritis conditions that are accompanied by an NADPH oxidase deficiency, methods and materials involved in treating, preventing, or delaying the onset of arthritis conditions that are accompanied by an NADPH oxidase deficiency, and methods and materials involved in identifying agonists and antagonists of NADPH oxidase activity.Type: GrantFiled: March 8, 2010Date of Patent: May 17, 2011Assignee: Arexis ABInventors: Rikard Holmdahl, Peter Olofsson
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Publication number: 20110111453Abstract: The present invention relates to enzyme compositions for high temperature saccharification of cellulosic material and to uses thereof.Type: ApplicationFiled: November 5, 2010Publication date: May 12, 2011Applicant: Novozymes, Inc.Inventors: Brett McBrayer, Tarana Shaghasi, Elena Vlasenko
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Patent number: 7939301Abstract: The present invention is directed towards an asymmetric enzymic process for preparing optically active organic compounds. The process according to the invention is carried out in what are termed miniemulsions. The invention also relates to an enzymic reaction mixture which exhibits a miniemulsion.Type: GrantFiled: November 4, 2005Date of Patent: May 10, 2011Assignee: Evonik Degussa GmbHInventors: Harald Gröger, Karlheinz Drauz, Hendrik Hüsken, Katharina Landfester
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Patent number: 7939305Abstract: The invention relates to genes which are coded for sequences specific to polyketide synthases (PKS). The thus synthesized PKS is characterized by the enzymatic capacity thereof to produce PUFAs (polyunsaturated fatty acids). The invention also relates to the identification of the corresponding DNA-sequences, in addition to the use of said nucleotide sequences for the production of recombined and/or transgenic organisms.Type: GrantFiled: April 8, 2005Date of Patent: May 10, 2011Assignee: Nutrinova Nutrition Specialties & Food Ingredients GmbHInventors: Markus Luy, Matthias Rüsing, Thomas Kiy
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Patent number: 7939250Abstract: The invention relates to a novel polypeptide vitamin K epoxide recycling polypeptide (VKORC1) as a target for coumarin and its derivatives. The invention further provides methods for identifying coumarin derivatives, and also claims VKORC1 polypeptides and VKORC1 nucleic acids containing a sequence abnormality associated with a VKORC1 associated deficiency such as warfarin resistance, wherein the VKORC1 polypeptides and VKORC1 nucleic acids can be used for diagnosing these deficiencies. Moreover, the invention relates to methods for identifying coumarin derivatives usable in pest control of rodents.Type: GrantFiled: October 14, 2004Date of Patent: May 10, 2011Assignees: Baxter International Inc., Baxter Healthcare S.A.Inventors: Johannes Oldenburg, Clemens R. Müller-Reible, Andreas Fregin, Simone Rost, Tim-Matthias Strom
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Patent number: 7939307Abstract: Modified CueO having excellent enzymatic activity and a composition for dyeing keratin fiber which contains the enzyme. A recombinant protein having an enzymatic activity for oxidizing p-phenylenediamine, the protein is obtained by removing from CueO at least one member selected from the group consisting of helix 5, helix 6, and helix 7; and the composition for dyeing keratin fiber containing the recombinant protein and an oxidation dye.Type: GrantFiled: April 14, 2006Date of Patent: May 10, 2011Assignees: National University Corporation Kanazawa University, Mandom CorporationInventors: Kaori Otsuka, Yoshio Tsujino, Yusuke Konno, Shinji Kurose, Kunishige Kataoka, Takeshi Sakurai
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Patent number: 7939320Abstract: The invention relates to a DNA vector comprising (a) a DNA sequence coding for the phytoene desaturase protein that is modified in one position by an amino add exchange providing resistance, and (b) a multiple cloning site into which any DNA sequence can be cloned. The invention also relates to the use of said DNA vector for transforming enkaryotic cells, transformation methods, and transgenic plant cells produced in said manner.Type: GrantFiled: October 12, 2006Date of Patent: May 10, 2011Assignee: Peter und Traudl Engelhorn-Stiftung zur Forderung der Biotechnologie und CentechnikInventor: Jens Steinbrenner
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Publication number: 20110104360Abstract: The invention provides an enzyme having a lignan-hydroxylating activity, particularly an enzyme capable of catalyzing the reaction of transferring a hydroxyl group to a lignan, an enzyme capable of catalyzing the hydroxylation of piperitol to 9-hydroxylpiperitol or pinoresinol to 9-hydroxylpinoresinol. The invention provides a polypeptide having a lignan-hydroxylating activity; a polynucleotide encoding the same; a vector or transformant comprising the polynucleotide; a method for producing a polypeptide having a lignan-hydroxylating activity which comprises using the transformant; and so on. The transformant wherein the polynucleotide is expressibly introduced is useful for the hydroxylation of a lignan or for the production of a product using the same in the food sector and a variety of industry sectors.Type: ApplicationFiled: December 17, 2008Publication date: May 5, 2011Applicant: SUNTORY HOLDINGS LIMITEDInventors: Eiichiro Ono, Asako Okada, Yuko Fukui