Oxidoreductase (1. ) (e.g., Luciferase) Patents (Class 435/189)
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Publication number: 20120226062Abstract: Mutant delta-9 elongases having the ability to convert linoleic acid [18:2, LA] to eicosadienoic acid [20:2, EDA] and/or ?-linolenic [18:3, ALA] to eicosatrienoic acid [20:3, ETrA] are disclosed herein. Isolated nucleic acid fragments and recombinant constructs comprising such fragments encoding mutant delta-9 elongases, along with a method of making long chain polyunsaturated fatty acids [“PUFAs”] using these mutant delta-9 elongases in oleaginous yeast are also disclosed.Type: ApplicationFiled: August 26, 2011Publication date: September 6, 2012Applicant: E. I. DU PONT DE NEMOURS AND COMPANYInventors: Michael W. Bostick, Hongxian He, Yougen Li, Quinn Qun Zhu
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Patent number: 8257952Abstract: The present disclosure provides engineered ketoreductase enzymes having improved properties as compared to a naturally occurring wild-type ketoreductase enzyme. Also provided are polynucleotides encoding the engineered ketoreductase enzymes, host cells capable of expressing the engineered ketoreductase enzymes, and methods of using the engineered ketoreductase enzymes to synthesize a variety of chiral compounds.Type: GrantFiled: December 23, 2010Date of Patent: September 4, 2012Assignee: Codexis, Inc.Inventors: Onorato Campopiano, Emily Mundorff, Birthe Borup, Rama Voladri
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Publication number: 20120219988Abstract: The present invention provides a highly efficient method of polymerizing a protein and a composition of polymerizing a protein. For example, the present invention provides a method comprising contacting a tyrosinase derived from nameko mushroom (Pholiota microspora) with a protein to be polymerized. The present invention also provides a composition for polymerizing a protein, comprising a tyrosinase derived from nameko mushroom (Pholiota microspora). In the present invention, glutamyltransferase can be optionally used. In addition, the protein to be polymerized includes, but not limited to, fish meat protein, egg white protein, a soybean protein, collagen, casein and gelatin.Type: ApplicationFiled: February 23, 2012Publication date: August 30, 2012Applicants: ISHIKAWA PREFECTURAL UNIVERSITY, SUGIYO CO., LTD.Inventors: Yasuko KONISHI, Fumio NODA, Ryota OHARA
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Publication number: 20120220010Abstract: The present invention relates generally to hydrogen production for use in fuel cells, foodstuffs and chemical production, and more particularly, to biologically and photosynthetically produced hydrogen. Specifically, disclosed is a method for producing bacteria and green alga that can produce hydrogen in quantities that exceed four hundred percent of the hydrogen produced by green alga in nature; thus, producing organisms which can serve as hydrogen generators for fuel cells, chemical production and numerous other applications.Type: ApplicationFiled: February 24, 2012Publication date: August 30, 2012Inventors: Scott Plummer, Mark Plummer
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Patent number: 8252559Abstract: A method and system for selectively fluorinating organic molecules on a target site wherein the target site is activated and then fluorinated are shown together with a method and system for identifying a molecule having a biological activity.Type: GrantFiled: February 4, 2009Date of Patent: August 28, 2012Assignee: The California Institute of TechnologyInventors: Rudi Fasan, Frances H. Arnold
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Patent number: 8252579Abstract: The present invention concerns a new method of preparation of a strain of evolved micro-organisms for the production of 1,2-propanediol by the metabolism of a simple carbon source, which method comprises the growth under selection pressure in an appropriate growth medium containing a simple carbon source of an initial bacterial strain that has undergone the deletion of the gene tpiA and the deletion of at least one gene involved in the conversion of methylglyoxal (propanal) into lactate, in order to cause, in said initial strain, the evolution of one or more genes involved in the biosynthesis pathway from DHAP to methylglyoxal and then to 1,2-propanediol towards evolved genes that possess an improved “1,2-propanediol synthase activity”, the resulting strain or strains of evolved micro-organisms possessing an improved “1,2-propanediol synthase activity” then being selected and isolated.Type: GrantFiled: January 12, 2005Date of Patent: August 28, 2012Assignee: Metabolic ExplorerInventors: Isabelle Meynial-Salles, Benjamin Gonzalez, Philippe Soucaille
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Publication number: 20120215018Abstract: The present invention relates to methods of synthesizing long-chain polyunsaturated fatty acids, especially eicosapentaenoic acid, docosapentaenoic acid and docosahexaenoic acid, in recombinant cells such as yeast or plant cells. Also provided are recombinant cells or plants which produce long-chain polyunsaturated fatty acids. Furthermore, the present invention relates to a group of new enzymes which possess desatorase or elongase activity that can be used in methods of synthesizing long-chain polyunsaturated fatty acids.Type: ApplicationFiled: April 16, 2012Publication date: August 23, 2012Inventors: Surinder Pal Singh, Stanley Suresh Robert, Peter David Nichols, Susan Irene Ellis Blackburn, Xue-Rong Zhou, James Robertson Petrie, Allan Graham Green
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Publication number: 20120210465Abstract: The present invention relates to a method for the production of unsaturated fatty acids with at least two double bonds. The invention furthermore relates to the use of nucleic acid sequences SEQ ID NO: 1, 3, 5, 9, and 11 encoding polypeptides having desaturase or elongase activity in the method and for generating a transgenic organism, preferably a transgenic plant or a transgenic microorganism, with an increased content of fatty acids, oils or lipids with unsaturated C18-, C20-, or C22-fatty acids, and to their homologs or derivatives, to gene constructs encompassing these genes, and to their use alone or in combination with biosynthesis genes of polyunsaturated fatty acids. The invention also relates to multiexpression cassettes for seed-specific expression, and to vectors or organisms which encompass a desaturase gene alone or in combination with further desaturases and/or elongase genes or homologs using said expression cassettes.Type: ApplicationFiled: January 2, 2012Publication date: August 16, 2012Applicant: BASF Plant Science GmbHInventors: Jens Lerchl, Andreas Renz, Ernst Heinz, Frederic Domergue, Ulrich Zähringer
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Patent number: 8241884Abstract: The present invention relates to ?17 desaturases, which have the ability to convert ?-6 fatty acids into their ?-3 counterparts (i.e., conversion of arachidonic acid [20:4, ARA] to eicosapentaenoic acid [20:5, EPA]). Isolated nucleic acid fragments and recombinant constructs comprising such fragments encoding ?17 desaturases along with a method of making long-chain polyunsaturated fatty acids (PUFAs) using these ?17 desaturases in oleaginous yeast are disclosed.Type: GrantFiled: May 20, 2009Date of Patent: August 14, 2012Assignee: E I du Pont de Nemours and CompanyInventors: Narendra S. Yadav, Zhixiong Xue, Quinn Qun Zhu
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Patent number: 8241879Abstract: The technology relates in part to biological methods for producing adipic acid and engineered microorganisms capable of such production.Type: GrantFiled: September 26, 2011Date of Patent: August 14, 2012Assignee: Verdezyne, Inc.Inventors: Stephen Picataggio, Tom Beardslee
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Patent number: 8241845Abstract: The invention provides an isolated, novel steroid 5?-reductase enzyme termed SRD5AIII. The protein has an estimated molecular weight of 37 kDa and is capable of converting testosterone to dihydrotestosterone at a pH of about 7.0. Also provided is a method for identifying inhibitors of SRD5AIII by contacting SRD5AIII with a test compound and measuring the activity of the enzyme. A reduced activity relative to a control indicates that the test compound is an inhibitor of SRD5AIII. A method is also provided for detecting androgen stimulated prostate cancer or recurrent prostate cancer in an individual. The method comprises obtaining a prostate biopsy from an individual and determining the level of expression of SRD5AIII gene or protein relative to a normal control. An increased expression of SRD5AIII relative to the control is indicative of androgen stimulated prostate cancer or recurrent prostate cancer.Type: GrantFiled: March 16, 2009Date of Patent: August 14, 2012Assignee: Health Research Inc.Inventors: James L. Mohler, Mark Titus, Olga Kozyreva, O. Harris Ford, III, Elsbieta Kawinski, Yun Li
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Publication number: 20120202256Abstract: A method of producing human metabolites of simvastatin or lovastatin, and more particularly, a method of producing human metabolites of simvastatin and lovastatin by using bacterial cytochrome P450 BM3(CYP102A1) or mutants thereof, and a composition and kit therefor.Type: ApplicationFiled: August 24, 2010Publication date: August 9, 2012Applicant: GLO BIOTECHInventors: Chul Ho Yun, Keon Hee Kim, Dong Hyun Kim, Ji Yeon Kang, Sun Ha Park
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Patent number: 8236551Abstract: The present application provides genetically modified fungal organisms that produce enzyme mixtures exhibiting enhanced hydrolysis of cellulosic material to glucose, enzyme mixtures produced by the genetically modified fungal organisms, and processes for producing glucose from cellulose using such enzyme mixtures.Type: GrantFiled: November 1, 2011Date of Patent: August 7, 2012Assignee: Codexis, Inc.Inventors: Ish Kumar Dhawan, Dipnath Baidyaroy, Andrew Shaw, Oleh Tanchak, Christopher Hill, Chengsong Liu, Amala Chokshi, Brian R. Scott
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Patent number: 8236540Abstract: The invention relates to the nucleotide and amino acid sequences, and to the activity and use, of the luciferases LuAL, Lu164, Lu16, Lu39, Lu45, Lu52 and Lu22.Type: GrantFiled: October 22, 2007Date of Patent: August 7, 2012Assignee: Bayer Intellectual Property GmbHInventors: Stefan Golz, Bernd Kalthof, Svetlana Markova, Ludmila Frank, Eugene Vysotski
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Publication number: 20120196347Abstract: Isolated nucleic acid fragments and recombinant constructs comprising such fragments encoding delta-8 desaturases along with a method of making long-chain polyunsaturated fatty acids (PUFAs) and using these delta-8 desaturases in plants.Type: ApplicationFiled: April 18, 2012Publication date: August 2, 2012Inventors: HOWARD GLENN DAMUDE, QUINN QUN ZHU
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Publication number: 20120190635Abstract: The present invention provides regio- and stereoselective oxidation of unactivated C—H bonds using an engineered mutant cytochrome P450 monooxygenase and an engineered substrate.Type: ApplicationFiled: September 27, 2010Publication date: July 26, 2012Applicant: THE REGENTS OF THE UNIVERSITY OF MICHIGANInventors: Shengying Li, David H. Sherman, John Montgomery, Mani R. Chaulagain, Allison R. Knauff
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Patent number: 8227206Abstract: The present invention disclosed a method to detect pregnancy and identify pseudopregnancy, pregnancy and pregnancy loss using ceruloplasmin as a non-invasive marker in animal urine samples. More specifically, this invention provide provides a method to use a non-invasive marker of inflammation, the acute phase protein ceruloplasmin, in urine samples to detect pregnancy and to distinguish between pregnancy and pseudopregnancy in mammals. The steps of this method include: collecting fresh urine sample from a mammal, adding a ceruloplasmin substrate to the urine sample, measuring oxidase activity of said ceruloplasmin through a sequential reading of absorbance of a photometric product using a spectrophotometer to determine the concentration of ceruloplasmin in the at least one urine sample.Type: GrantFiled: March 23, 2012Date of Patent: July 24, 2012Assignee: Memphis Zoo, Inc.Inventor: Erin Lynn Willis
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Patent number: 8227227Abstract: The present invention relates to cells producing at least one polypeptide of interest and expressing one or more recombinant nuclease encoding gene(s) thereby producing the nuclease(s), and methods for producing a polypeptide of interest essentially free from contaminating DNA, said method comprising the steps of: (a) cultivating a cell that produces at least one polypeptide of interest and expresses one or more recombinant nuclease encoding gene(s) thereby producing the nuclease(s); and (b) isolating the polypeptide of interest.Type: GrantFiled: November 30, 2007Date of Patent: July 24, 2012Assignee: Novozymes A/SInventors: Michael Dolberg Rasmussen, Jon Martin Persson
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Patent number: 8227228Abstract: The present invention relates to novel D-amino acid oxidase isolated and purified from Candida intermedia, a gene encoding the D-amino acid oxidase, a recombinant plasmid containing the gene, and a transformant into which the D-amino acid oxidase gene has been introduced, as well as a production method of D-amino acid oxidase including culturing the transformant. Moreover, the present invention relates to a production method of L-amino acids, 2-oxo acids or cyclic imines, which include reacting racemic amino acids with the D-amino acid oxidase, more preferably, a production method of L-amino acids, which includes reacting racemic amino acid with the D-amino acid oxidase, amino acid dehydrogenase and an enzyme having a coenzyme-regenerating activity. According to the present invention, L-amino acids, 2-oxo acids or cyclic imines can be produced with good efficiency in an industrial scale.Type: GrantFiled: August 2, 2006Date of Patent: July 24, 2012Assignee: Kaneka CorporationInventors: Hiroyuki Kanamaru, Makoto Ueda, Ryuuji Miki, Hirokazu Nanba
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Patent number: 8227218Abstract: The invention relates to a method for the enzymatic reduction of alkyne derivatives of the formula (1), wherein R1 represents H, C1-C6-alkyl, C2-C6-alkenyl, or an optionally substituted carbocyclic or heterocyclic, aromatic or non-aromatic group, R2 represents H, C1-C6-alkyl, C2-C6-alkenyl, by reaction in the presence of special reductases.Type: GrantFiled: August 30, 2007Date of Patent: July 24, 2012Assignee: BASF SEInventors: Rainer Stürmer, Bernhard Hauer, Bettina Rosche, André Mueller
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Patent number: 8227229Abstract: The present disclosure provides engineered ketoreductase enzymes having improved properties as compared to a naturally occurring wild-type ketoreductase enzyme. Also provided are polynucleotides encoding the engineered ketoreductase enzymes, host cells capable of expressing the engineered ketoreductase enzymes, and methods of using the engineered ketoreductase enzymes to synthesize chiral compounds.Type: GrantFiled: May 18, 2011Date of Patent: July 24, 2012Assignees: Codexis, Inc., Pfizer, Inc.Inventors: Jack Liang, Stephane J. Jenne, Emily Mundorff, Rama Voladri, James LaLonde, Gjalt W. Huisman
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Publication number: 20120184015Abstract: Nucleic acids encoding cytochrome P450 variants are provided. The cytochrome P450 variants of have a higher alkane-oxidation capability, alkene-oxidation capability, and/or a higher organic-solvent resistance than the corresponding wild-type or parent cytochrome P450 enzyme. A preferred wild-type cytochrome P450 is cytochrome P450 BM-3. Preferred cytochrome P450 variants include those having an improved capability to hydroxylate alkanes and epoxidate alkenes comprising less than 8 carbons, and have amino acid substitutions corresponding to V78A, H236Q, and E252G of cytochrome P450 BM-3. Preferred cytochrome P450 variants also include those having an improved hydroxylation activity in solutions comprising co-solvents such as DMSO and THF, and have amino acid substitutions corresponding to T235A, R471A, E494K, and S1024E of cytochrome P450 BM-3.Type: ApplicationFiled: December 12, 2011Publication date: July 19, 2012Applicant: THE CALIFORNIA INSTITUTE OF TECHNOLOGYInventors: Edgardo T. Farinas, Frances H. Arnold, Ulrich Schwaneberg, Anton Glieder
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Publication number: 20120184006Abstract: In some embodiments, the present invention relates to isolated enzymes useful in reducing a fatty acyl-CoA to a corresponding fatty alcohol in a single biosynthetic step, polynucleotides encoding the enzymes, and methods for making and using these polynucleotides and enzymes. In some embodiments, the invention provides for isolated or recombinant enzymes capable of reducing a fatty acyl-CoA to a fatty alcohol. In still another embodiment, the invention provides for isolated or recombinant polynucleotides encoding an enzyme capable of reducing a fatty acyl-CoA to a fatty alcohol. In other embodiments, the invention provides for methods of making or using enzymes capable of reducing fatty acyl-CoA to a fatty alcohol, and methods of making using polynucleotides that encode the enzymes.Type: ApplicationFiled: January 14, 2012Publication date: July 19, 2012Applicant: UTAH STATE UNIVERSITYInventors: Robert M. Willis, Bradley D. Wahlen, Lance C. Seefeldt, Brett M. Barney
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Publication number: 20120183993Abstract: The invention relates to a process for the preparation of a fermentation product from ligno-cellulosic material, comprising the following steps: a) optionally pre-treatment b) optionally washing; c) enzymatic hydrolysis; d) fermentation; and e) optionally recovery of a fermentation product; wherein in step c) an enzyme composition is used that has a temperature optimum of 55 degrees C. or more, the hydrolysis time is 40 hours or more and the temperature is 50 degrees C. or more.Type: ApplicationFiled: October 5, 2010Publication date: July 19, 2012Applicant: DSM IP ASSETS B.V.Inventors: Johannes Petrus Smits, Elisabeth Maria Gierveld, Fop Cornelis Van Der Hor
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Publication number: 20120185965Abstract: The invention in principle pertains to the field of recombinant manufacture of fatty acids. It provides novel nucleic acid molecules comprising nucleic acid sequences encoding fatty acid desaturases, elongases, acyltransferases, terminator sequences and high expressing seed-specific promoters operatively linked to the said nucleic acid sequences wherein nucleic acid expression enhancing nucleic acids (NEENAs) are functionally linked to said promoters.Type: ApplicationFiled: August 27, 2010Publication date: July 19, 2012Applicant: BASF Plant Science Company GmbHInventors: Toralf Senger, Jörg Bauer, Josef Martin Kuhn
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Patent number: 8222016Abstract: Disclosed are: a recombinant C-terminal ?-amidated enzyme derivative which lacks the formation of at least one disulfide bond among five disulfide bonds occurring in a C-terminal ?-amidated enzyme derived from Xenopus laevis; DNA encoding the derivative; an expression vector carrying the DNA; a bacterium Escherichia coli transformed with the expression vector; and a method for producing the derivative by using the bacterium Escherichia coli.Type: GrantFiled: June 27, 2008Date of Patent: July 17, 2012Assignee: Daiichi Sankyo Company, LimitedInventors: Takeshi Okumura, Kazuaki Furukawa, Masayuki Yabuta
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Patent number: 8222017Abstract: Methods for the evolution of NADPH binding ketol-acid reductoisomerase enzymes to acquire NADH binding functionality are provided. Specific mutant ketol-acid reductoisomerase enzymes isolated from Pseudomonas that have undergone co-factor switching to bind NADH are described.Type: GrantFiled: November 15, 2011Date of Patent: July 17, 2012Assignee: Butamax(TM) Advanced Biofuels LLCInventors: Yougen Li, Der-Ing Liao, Mark J. Nelson, Daniel P. Okeefe, Jean-Francois Tomb
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Patent number: 8222010Abstract: Disclosed herein are methods of manufacturing renewable chemicals through the manufacture of novel triglyceride oils followed by chemical modification of the oils. Methods such as transesterification, hydrogenation, hydrocracking, deoxygenation, isomerization, interesterification, hydroxylation, hydrolysis and saponification are disclosed. Novel oils containing fatty acid chain lengths of C8, C10, C12 or C14 are also disclosed and are useful as feedstocks in the methods of the invention.Type: GrantFiled: December 29, 2010Date of Patent: July 17, 2012Assignee: Solazyme, Inc.Inventors: Scott Franklin, Aravind Somanchi, Karen Espina, George Rudenko, Penelope Chua
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Publication number: 20120178141Abstract: Briefly described, embodiments of this disclosure include polynucleotides that encode mutant Cnidarian luciferases that exhibit modulated properties as compared to the corresponding wild-type luciferases, and the modulated properties include at least one of: modulated stability; enhanced light output; and modulated emission maximum. Embodiments of the present disclosure also include polypeptides or fragments thereof encoded by the polynucleotides, constructs including the polynucleotide, expression cassettes, cells, methods of producing the polynucleotides and polypeptides, antibodies, transgenic cells and/or animals, kits, and the like.Type: ApplicationFiled: March 22, 2012Publication date: July 12, 2012Applicants: The Regents of the University of California, The Board of Trustees of the Leland Stanford Junior UniversityInventors: Sanjiv S. Gambhir, Andreas M. Loening, Anna M. Wu
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Patent number: 8216815Abstract: The disclosure relates to methods of producing fatty alcohols from recombinant host cells comprising genes encoding heterologous fatty acyl-CoA reductase (FAR) enzymes. The disclosure further relates to FAR enzymes and functional fragments thereof derived from marine bacterium and particularly marine gamma proteobacterium such as Marinobacter and Oceanobacter; polynucleotides encoding the FAR enzymes and vectors and host cells comprising the same.Type: GrantFiled: June 29, 2010Date of Patent: July 10, 2012Assignee: Codexis, Inc.Inventors: Robert McDaniel, Behnaz Behrouzian, Louis Clark, Douglas Hattendorf, Fernando Valle
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Patent number: 8216811Abstract: Described herein are novel nucleic acids, proteins and methods that can be used to provide new catalysts with desirable traits for industrial processes. In particular, novel reductases isolated from the environment using PCR methods are described.Type: GrantFiled: March 4, 2011Date of Patent: July 10, 2012Assignee: Danisco US Inc.Inventors: Mark Donnelly, William H. Eschenfeldt, Jonathan Trent
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Patent number: 8216550Abstract: Novel luciferins, methods of making luciferins, and uses of the same are disclosed.Type: GrantFiled: February 14, 2011Date of Patent: July 10, 2012Assignee: University of MassachusettsInventor: Stephen C. Miller
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Publication number: 20120174242Abstract: An isolated polynucleotide encoding a modified luciferase polypeptide and substrates. The OgLuc variant polypeptide has at least 60% amino acid sequence identity to SEQ ID NO: 1 and at least one amino acid substitution at a position corresponding to an amino acid in SEQ ID NO: 1. The OgLuc variant polypeptide has at least one of enhanced luminescence, enhanced signal stability, and enhanced protein stability relative to the corresponding polypeptide of the wild-type Oplophorus luciferase.Type: ApplicationFiled: November 2, 2011Publication date: July 5, 2012Inventors: Brock Binkowski, Lance P. Encell, Mary Hall, Matthew B. Robers, Michael R. Slater, Keith V. Wood, Monika G. Wood
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Publication number: 20120171735Abstract: The invention relates to production of triacylglycerols in cells.Type: ApplicationFiled: June 15, 2010Publication date: July 5, 2012Applicant: Massachusetts Institute of TechnologyInventors: Anthony John Sinskey, Daniel MacEachran, Kazuhiko Kurosawa, Paolo Boccazzi, Jason W. Holder
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Publication number: 20120172244Abstract: Genetic biomarkers for left side colon cancer (LCC) (such as expression levels of an RNA transcript or expression product of NOX4, MMP3, or a combination) and right side colon cancer (RCC) (such as expression levels of an RNA transcript or expression product of CDCX2, FAM69A, or a combination), are disclosed. Methods for using the biomarkers in providing a prognosis of relapse-free survival probability in patients having LCC or RCC are also presented. Prognostic panels using gene expression values of the biomarkers are also presented. Computer implemented methods employing the biomarkers, and as well as for determining relapse-free survival probability in a patient having RCC or LCC are provided. A genetic method for classifying a colon cancer tissue as a RCC or as a LCC is also disclosed.Type: ApplicationFiled: December 20, 2011Publication date: July 5, 2012Inventors: Steven Buechler, Amanda Hummon
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Publication number: 20120164696Abstract: The invention relates to recombinant expression of a variant form of a fungal C1 strain ?-glucosidase. The invention also relates to the generation of fermentable sugars from biomass and the production of biofuels by fermentation of the sugars using genetically modified organisms expressing the ?-glucosidase variant. The invention provides methods for producing a fermentable sugar, such as glucose, from cellobiose by contacting cellobiose with a recombinant ?-glucosidase variant protein, such as a variant protein secreted by a recombinant host cell into culture medium. Methods of the invention may be used for conversion of a biomass substrate to a fermentable sugar, and ultimately to ethanol or other biofuel.Type: ApplicationFiled: March 9, 2012Publication date: June 28, 2012Applicant: Codexis, Inc.Inventors: Jie Yang, Xiyun Zhang, Attila Andor
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Publication number: 20120164678Abstract: The invention relates to recombinant expression of a steviol or steviol glycosides biosynthetic pathway enzymes in cells and the production of steviol or steviol glycosides.Type: ApplicationFiled: November 29, 2011Publication date: June 28, 2012Applicant: Massachusetts Institute of TechnologyInventors: Gregory Stephanopoulos, Parayil K. Ajikumar
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Publication number: 20120159838Abstract: The present invention relates to a process for producing enzymes and single cell oil. The process comprises that microorganisms capable of producing both single cell oil and enzymes are cultivated under conditions suitable for single cell oil production and enzyme production in a single cell oil production process. A microorganism culture comprising single cell oil and enzymes is obtained and at least part of the microorganism culture, of the supernatant and/or microorganism cells separated from the microorganism culture, of protein fraction enriched from the supernatant, and/or of protein fraction obtained from the cells is used as an enzyme preparation or as a source of enzymes. Single cell oil is recovered from the microorganism cells and used as biofuel, component of biofuel or as a starting material for biofuel production. Enzymes produced according to the process are used in the same or in another industrial process.Type: ApplicationFiled: December 21, 2011Publication date: June 28, 2012Applicant: NESTE OIL OYJInventors: Annika Malm, Simo Laakso, Ossi Pastinen, Heidi Kahelin, Miia Mujunen
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Patent number: 8206957Abstract: A process for preparing optically active saturated aldehydes or alcohols of the formula (2) from ?,?-unsaturated aldehydes of the formula (1) by reduction in the presence of an enoate reductase (i) having the polypeptide sequence SEQ ID No. 1 or 2, or (ii) having a polypeptide sequence which is at least 80% identical to the sequence of SEQ ID No. 1 or 2.Type: GrantFiled: December 19, 2006Date of Patent: June 26, 2012Assignee: BASF SEInventors: Andreas Schädler, Thomas Friedrich, Rainer Stürmer, Sabine Rinck
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Patent number: 8206961Abstract: A codon optimized and stabilized luciferase gene based upon the sequence of the natural luciferase gene isolated from Luciola cruciata (Japanese firefly) and a novel recombinant DNA characterized by incorporating this new gene coding for a novel luciferase into a vector DNA for improved activities in mammalian cells, are disclosed. This new luciferase exhibits long-wavelength light emission, as well as improved thermostability and higher expression levels in mammalian cell systems, compared to native luciferase.Type: GrantFiled: May 24, 2010Date of Patent: June 26, 2012Assignee: Marker Gene Technologies, Inc.Inventors: Daniel J. Coleman, John J. Naleway, Gabriele M. Cook
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Patent number: 8206954Abstract: An L-amino acid is produced by culturing a bacterium belonging to the family Enterobacteriaceae, which has an L-amino acid-producing ability and inherently has a native activity of a glucose dehydrogenase that uses pyrroloquinoline quinone as a coenzyme, but has been modified so that the activity of the glucose dehydrogenase is reduced, in a medium, and collecting the L-amino acid from the medium.Type: GrantFiled: March 1, 2011Date of Patent: June 26, 2012Assignee: Ajinomoto Co., Inc.Inventors: Rie Takikawa, Yoshihiko Hara, Gen Nonaka, Kazuhiro Takumi
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Patent number: 8206959Abstract: The invention is related to processing enzyme comprising an N-terminally attached tag derived from highly basic proteins from thermophilic bacteria. The processing enzymes are useful for modifying proteins. They can be produced in high yields and can be effectively separated from the modified protein after use.Type: GrantFiled: November 11, 2010Date of Patent: June 26, 2012Assignee: Novo Nordisk Health Care AGInventor: Allan Christian Shaw
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Patent number: 8206970Abstract: Methods for the fermentive production of four carbon alcohols are provided. Specifically, butanol, preferably 2-butanol is produced by the fermentive growth of a recombinant bacteria expressing a 2-butanol biosynthetic pathway. The recombinant microorganisms and methods of the invention can also be adapted to produce 2-butanone, an intermediate in the 2-butanol biosynthetic pathways disclosed herein.Type: GrantFiled: April 30, 2007Date of Patent: June 26, 2012Assignee: Butamax(TM) Advanced Biofuels LLCInventors: Andrew C. Eliot, Vasantha Nagarajan, Lixuan Lisa Huang
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Patent number: 8206967Abstract: The present invention relates to compositions and methods for producing recombinant human thrombin using recombinant ecarin.Type: GrantFiled: July 3, 2008Date of Patent: June 26, 2012Assignee: MedImmune LimitedInventors: Anna Harrysson, Ann Lövgren
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Publication number: 20120159676Abstract: An object of the present invention is to provide DNA of a glycoalkaloid biosynthetic enzyme of a solanaceous plant (Solanaceae) such as a potato. The present invention relates to a protein having glycoalkaloid biosynthetic enzyme activity of a solanaceous plant such as a potato and a method for producing/detecting a novel organism using a gene encoding the protein.Type: ApplicationFiled: August 30, 2010Publication date: June 21, 2012Applicant: KIRIN HOLDINGS KABUSHIKI KAISHAInventors: Naoyuki Umemoto, Katsunori Sasaki
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Publication number: 20120157326Abstract: The invention is in the field of nucleic acid amplification, hi particular, methods are described which utilise stem primers that improve the rapid and specific amplification of a test sample.Type: ApplicationFiled: June 15, 2010Publication date: June 21, 2012Inventors: Laurence Carlo Tisi, Olga Gandelmann, Guy Kiddle, Cathal Joseph McElgunn
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Publication number: 20120159660Abstract: The amino acid and nucleic acid sequences of a ?5-desaturase enzyme and a ?8-desaturase enzyme are disclosed. The nucleic acid sequences can be used to design recombinant DNA constructs and vectors. These vectors can then be used to transform various organisms, including for example, plants and yeast. The transformed organisms will then produce polyunsaturated fatty acids. The amino acid sequences are useful for generating enzyme-specific antibodies that are useful for identifying the desaturases.Type: ApplicationFiled: February 27, 2012Publication date: June 21, 2012Inventors: John A. Browse, James G. Wallis, Jennifer L. Watts
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Publication number: 20120151630Abstract: The invention provides isolated and at least partially-purified dicamba-degrading enzymes, isolated DNA molecules coding for dicamba-degrading enzymes, DNA constructs coding for dicamba-degrading enzymes, transgenic host cells comprising DNA coding for dicamba-degrading enzymes, and transgenic plants and plant parts comprising one or more cells comprising DNA coding for dicamba-degrading enzymes. Expression of the dicamba-degrading enzymes results in the production of dicamba-degrading organisms, including dicamba-tolerant plants. Finally, the invention provides a method of selecting transformed plants and plant cells based on dicamba tolerance and a method of selecting or screening transformed host cells, intact organisms and parts of organisms based on the fluorescence of 3,6-dichlorosalicylic acid produced as a result of dicamba degradation.Type: ApplicationFiled: January 12, 2012Publication date: June 14, 2012Applicant: BOARD OF REGENTS OF UNIVERSITY OF NEBRASKAInventors: Donald P. Weeks, Xiao-Zhuo Wang, Patricia L. Herman
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Publication number: 20120149073Abstract: The disclosure relates to engineered ketoreductase polypeptides and processes of using the polypeptides for production of phenylephrine.Type: ApplicationFiled: August 19, 2010Publication date: June 14, 2012Applicant: CODEXIS, INC.Inventors: Oscar Alvizo, Steven J. Collier, Joerg Hennemann, Seong Ho Oh, Wenjuan Zha
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Patent number: RE43497Abstract: The present invention relates to methods for screening molecules and methods to detect protein-protein interactions and means used therein. More specifically, the present invention relates to methods for screening candidate drugs for treating or detecting MIF (macrophage migration inhibitor factor) related diseases. In certain aspects, the present invention involves detecting MIF/Jab1 (c-Jun activation domain binding protein) interactions as a basis for modulating cellular regulatory pathways and for identifying candidate drugs for MIF-related diseases. The invention also provides methods for the identification of molecules which dissociate or prevent interaction or binding between MIF and Jab1.Type: GrantFiled: November 2, 2000Date of Patent: June 26, 2012Assignee: Fraunhofer-Gesellschaft zur Forderung der Angewandten Forschung E.V.Inventors: Herwig Brunner, Jürgen Bernhagen, Robert Kleemann, Ralf Mischke, Afroditi Kapurniotu