Transferase Other Than Ribonuclease (2.) Patents (Class 435/193)
  • Patent number: 7906460
    Abstract: The present invention provides mutant nucleotidylyl-transferases, such as Ep, having altered substrate specificity; methods for their production; and methods of producing nucleotide sugars, which utilize these nucleotidylyl-transferases. The present invention also provides methods of synthesizing desired nucleotide sugars using natural and/or modified Ep or other nucleotidyltransferases; and nucleotide sugars synthesized by the present methods. The present invention further provides new glycosyl phosphates, and methods for making them.
    Type: Grant
    Filed: September 28, 2006
    Date of Patent: March 15, 2011
    Assignee: Sloan-Kettering Institute for Cancer
    Inventors: Jon Thorson, Dimitar B. Nikilov
  • Patent number: 7906307
    Abstract: The present invention relates to a method for the production of a variant lipid acyltransferase comprising the steps of: (i) selecting a parent enzyme which is a lipid acyltranserase enzyme; (ii) modifying one or more amino acids to produce a variant lipid acyltransferase; (iii) testing the activity of the variant lipid acyltransferase on a galactolipid and/or phospholipid and/or triglyceride substrate; (iv) selecting a variant enzyme with enhanced activity towards galactolipids compared with the parent enzyme; (v) providing a Bacillus licheniformis cell; (vi) transforming the Bacillus licheniformis cell with a heterologous nucleotide sequence encoding said variant lipid acyltransferase; and (iii) expressing said variant lipid acyltransferase in the cell under the control of a promoter sequence. The variant lipid acyltransferase can undergo post-translations modification, truncation and/or clipping, i.e., to remove a signal peptide.
    Type: Grant
    Filed: September 7, 2007
    Date of Patent: March 15, 2011
    Assignee: Danisco A/S
    Inventors: Jørn Borch Søe, Jørn Dalgaard Mikkelson, Arno de Kreij
  • Publication number: 20110059869
    Abstract: An object of the invention is to provide a method for increasing enzymatic reactivity to a target substance immobilized on a support; and a method for reducing or suppressing an inhibitory effect of a support on enzymatic reaction. The above object is achieved by a method for increasing enzymatic reactivity to a target substance immobilized on a support by allowing at least one substance selected from the group consisting of saccharides, amino acids, polyhydric alcohols and derivatives thereof to exist; and a method for reducing or suppressing an inhibitory effect of a support immobilized with a target substance on enzymatic reactivity to the target substance by allowing at least one substance selected from the group consisting of saccharides, amino acids, polyhydric alcohols and derivatives thereof to exist.
    Type: Application
    Filed: February 27, 2009
    Publication date: March 10, 2011
    Inventors: Miki Kojima, Piero Carninci, Yoshihide Hayashizaki, Matthias Harbers
  • Publication number: 20110059504
    Abstract: Disclosed herein are methods and compositions related to engineered fragments of the human transglutaminase-related protein family, described herein as engineered transglutaminase barrel proteins (ETBPs), that have utility as high affinity, high selectivity target-binding proteins offering advantages as antibody equivalents for therapeutic, analytical, manufacturing and research purposes. ETBPs differ from naturally occurring human transglutaminase fragments by the addition, deletion, replacement and/or substitution of the naturally occurring amino acid sequence. ETBPs can be easily expressed in prokaryotic cells and in many cases can be purified by a simple solubilization and precipitation method.
    Type: Application
    Filed: January 5, 2009
    Publication date: March 10, 2011
    Inventors: Elena V. Getmanova, Alexander Kovtur, Lin Sun, Edward Fritsch, Brian Seed
  • Publication number: 20110061130
    Abstract: The present disclosure relates to the isolation, purification, and characterization of a diacylglycerol acyltransferase 2 (DGAT2), and genes encoding DGAT2, from algae. DGAT2 can incorporate very long chain polyunsaturated fatty acids in to triacylglycerol more efficiently than DGAT1. The disclosure concerns methods of regulating seed oil content, fatty acid synthesis and fatty acid composition using the DGAT2 gene and to tissues and plants transformed with the gene. The disclosure also relates to transgenic plants, plant tissues and plant seeds having a genome containing an introduced DNA sequence of the disclosure, and a method of producing such plants and plant seeds.
    Type: Application
    Filed: December 16, 2008
    Publication date: March 10, 2011
    Inventors: Jitao Zou, Jingyu Xu, Zhifu Zheng
  • Publication number: 20110059473
    Abstract: The present invention relates to proteins with pteridine glycosyltransferase activity and an analysis method using the same. Since the proteins glycate tetrahydrobiopterin selectively through an enzyme reaction, the method enables quantitative analysis of tetrahydrobiopterin and oxides thereof at the same time or quantitative analysis of tetrabiopterin selectively.
    Type: Application
    Filed: March 30, 2009
    Publication date: March 10, 2011
    Applicant: INJE UNIVERSITY INDUSTRY- ACADEMIC COOPERATION FOUNDATION
    Inventors: Young Shik Park, Jin Han
  • Patent number: 7901924
    Abstract: Methods of increasing the cellular pool of A-CoA and thus driving the metabolic pathways in the direction of A-CoA containing metabolites by overexpressing rate limiting enzymes in A-CoA synthesis. Methods of increasing intracellular levels of CoA and A-CoA through genetic engineering of bacterial strains in conjunction with supplementation with precursor molecules.
    Type: Grant
    Filed: December 19, 2008
    Date of Patent: March 8, 2011
    Assignee: Rice University
    Inventors: Ka-Yiu San, George Nelson Bennett, Ravishankar V. Vadali
  • Patent number: 7901912
    Abstract: The present invention provides a method for enzymatically producing uridine 5?-diphospho-N-acetylgalactosamine (UDP-GalNAc) (which is an important substrate for oligosaccharide synthesis) from uridine 5?-triphosphate (UTP) and N-acetylgalactosamine 1-phosphate (GalNAc 1-P), the method including using, as an enzyme, uridine 5?-diphospho-N-acetylglucosamine pyrophosphorylase (UDP-GlcNAc pyrophosphorylase) derived from a microorganism (exclusive of a pathogenic microorganism). The GalNAc 1-P employed can be prepared from N-acetylgalactosamine and a phosphate donor in a reaction system by use of N-acetylgalactosamine kinase. According to the present invention, uridine 5?-diphospho-N-acetylgalactosamine can be efficiently produced by use of a relatively inexpensive substrate.
    Type: Grant
    Filed: October 18, 2005
    Date of Patent: March 8, 2011
    Assignee: Yamasa Corporation
    Inventors: Kiyoshi Okuyama, Toshitada Noguchi
  • Patent number: 7901917
    Abstract: Compositions comprising truncated tryptophanyl-tRNA synthetase polypeptides useful for regulating angiogenesis, as well as nucleic acids encoding such tRNA synthetase polypeptides are described. Methods of making and using such compositions are also disclosed.
    Type: Grant
    Filed: April 17, 2009
    Date of Patent: March 8, 2011
    Assignee: The Scripps Research Institute
    Inventors: Paul Schimmel, Keisuke Wakasugi
  • Patent number: 7901928
    Abstract: Two acyltransferases are provided, suitable for use in the manufacture of microbial oils enriched in omega fatty acids in oleaginous yeast (e.g., Yarrowia lipolytica). Specifically, the genes encoding phophatidylcholine-diacylglycerol acyltransferase (PDAT) and diacylglycerol acyltransferase (DGAT2) have been isolated from Y. lipolytica. These genes encode enzymes that participate in the terminal step in oil biosynthesis in yeast. Each is expected to play a key role in altering the quantity of polyunsaturated fatty acids produced in oils of oleaginous yeasts.
    Type: Grant
    Filed: March 11, 2009
    Date of Patent: March 8, 2011
    Assignee: E. I. du Pont de Nemours and Company
    Inventors: Narendra S. Yadav, Hongxiang Zhang
  • Publication number: 20110053245
    Abstract: This invention relates generally to enzymes, polynucleotides encoding the enzymes, the use of such polynucleotides and polypeptides and more specifically to enzymes having isomerase activity, e.g., racemase activity, e.g., amino acid racemase activity, alanine racemase activity, and/or epimerase activity, and/or catalyze the re-, arrangement of atoms within a molecule, catalyze the conversion of one isomer into another, catalyze the conversion of an optically active substrate into a raceme, which is optically inactive, catalyze the interconversion of substrate enantiomers, catalyze the stereochemical inversion around the asymmetric carbon atom in a substrate having only one center of asymmetry, catalyze the stereochemical inversion of the configuration around an asymmetric carbon atom in a substrate having more than one asymmetric center, and/or catalyze the racemization of amino acids.
    Type: Application
    Filed: December 22, 2008
    Publication date: March 3, 2011
    Applicant: VERENIUM CORPORATION
    Inventors: David P. Weiner, Peter Luginbuhl, Analia Bueno, Joslin M. Cuenca, Mervyn L. De Souza, Sherry Kollmann
  • Publication number: 20110055963
    Abstract: A method of acylating the 3? position of anthocyanin using an enzyme that transfers an aromatic acyl group to a sugar at the 3? position of anthocyanin or a gene encoding the enzyme.
    Type: Application
    Filed: October 28, 2005
    Publication date: March 3, 2011
    Applicant: International Flower Developments Proprietary Limited
    Inventors: Yoshikazu Tanaka, Yukihisa Katsumoto, Masako Mizutani, Yuko Fukui, Junichi Togami
  • Publication number: 20110053292
    Abstract: There is provided a novel method for amplifying mass spectrometric signals. More particularly, a novel method for detecting signals of a target molecule includes: i) allowing a test sample, in which it is required to determine whether or not a target molecule is present, to be contact with a gold particle whose surface is modified to selectively bind to the target molecule, ii) allowing a low molecular molecule engrafted to the gold particle to generate mass spectrometric signals after the interaction, such as binding, between the gold particle and the target molecule, and iii) amplifying the mass spectrometric signals by generating a great deal of mass spectrometric signals of the low molecular molecule even in the presence of a trace of the target molecule. Also, the assay system using the method and the gold particle prepared in the method are provided.
    Type: Application
    Filed: April 10, 2009
    Publication date: March 3, 2011
    Applicant: PROBIOND CO., LTD.
    Inventors: Hyung-Soon Park, Sang-Wan So, Woon-Seok Yeo, Soo-Jae Lee, Jung-Rok Lee, Ju-Hee Lee, Kwang-Pyo Kim
  • Patent number: 7897369
    Abstract: The present invention provides isolated polynucleotides and isolated polypeptides. The polypeptides of the present invention have isoprenoid wax ester synthase activity or isoprenoid acyl CoA-synthetase activity. The present invention also includes methods of using the polynucleotides and polypeptides of the present invention. For instance, the methods include producing biodiesel and producing wax esters.
    Type: Grant
    Filed: August 27, 2008
    Date of Patent: March 1, 2011
    Assignee: Regents of the University of Minnesota
    Inventors: Claudia Schmidt-Dannert, Erik K. Holtzapple
  • Publication number: 20110045542
    Abstract: The present invention relates to a reaction mixture for the amplification of nucleic acids, the non-methylated cytosine bases of which have been converted to uracil bases by means of a bisulfition reaction. The invention also discloses methods for amplifying bisulfited nucleic acid and for determining the nucleic acid methylation state, and also kits based on the reaction mixture according to the invention.
    Type: Application
    Filed: February 6, 2009
    Publication date: February 24, 2011
    Inventors: Christian Korfhage, Dirk Loeffert, Ralf Peist, Nicolas Rudinger
  • Patent number: 7892771
    Abstract: A crystalline structure of glutaminyl cyclase (QC) is described. Also described are the methods of preparing the crystalline structure of QC and the methods for identifying candidate inhibitors of QC. In addition, a structural basis for the rational design or identification of new inhibitors that may be used to treat QC-associated disorders is also described.
    Type: Grant
    Filed: April 24, 2009
    Date of Patent: February 22, 2011
    Assignee: Academia Sinica
    Inventors: Andrew H.-J. Wang, Kai-Fa Huang
  • Patent number: 7893234
    Abstract: An isolated polypeptide comprising an amino acid sequence that is at least 62% identical to SEQ ID NO:2. The polypeptide, when present in a cell, increases the cell's ability to tolerate glyphosate. Also disclosed are related nucleic acid, antibody, vector, transgenic plant, as well as uses thereof.
    Type: Grant
    Filed: May 11, 2007
    Date of Patent: February 22, 2011
    Assignee: Si Chuan Heben Biotic Engineering Co. Ltd.
    Inventors: Min Lin, Kexuan Tang, Yiping Wang, Jin Wang, Yu Zhu
  • Patent number: 7892838
    Abstract: The invention relates to a method for altering a protein X such as to modify the characteristics thereof by a) obtaining the mutants X* of the sequence coding for protein X, by means of aleatory mutagenesis, b) transformation of cells with a phenotype [P-] with vectors comprising the mutated nucleic acids obtained in step (a) which code for proteins X*, where P-signifies that said cells are auxotrophic for substance P, P begin the product of the action of X on the natural substrate thereof S, c) culturing said cells in a medium comprising a substrate S*, S* being an analogue of the natural substrate S of the protein X, d) selection of the cells [P-:: X*] which have survived step c) in which the proteins X* can biosynthesise the product P from the substrate S*.
    Type: Grant
    Filed: April 13, 2007
    Date of Patent: February 22, 2011
    Assignees: Institut Pasteur, Centre National de la Recherche Scientifique (CNRS)
    Inventors: Pierre-Alexandre Kaminski, Philippe Marliere
  • Publication number: 20110039732
    Abstract: The present invention provides methods for selectively amplifying a target population of nucleic acid molecules in a population of RNA template molecules (e.g., all mRNA molecules expressed in a cell type except for the most highly expressed mRNA species). The present invention also provides a first population of oligonucleotides including the nucleic acid sequences set forth in SEQ ID NOS:1-749 and a second population of oligonucleotides including the nucleic acid sequences set forth in SEQ ID NOS:750-1498. The first population of oligonucleotides can be used, for example, to prime the synthesis of first strand cDNA molecules complementary to mRNA molecules isolated from mammalian cells without priming the synthesis of cDNA molecules complementary to ribosomal RNA molecules.
    Type: Application
    Filed: April 26, 2010
    Publication date: February 17, 2011
    Applicant: LIFE TECHNOLOGIES CORPORATION
    Inventors: Christopher Raymond, Christopher Armour, John Castle
  • Patent number: 7888090
    Abstract: The invention relates to AGT mutants showing, when compared to the wild type human AGT, two or more advantageous properties selected from (a) reduced DNA interaction; (b) localisation of the expressed protein in eukaryotic cells that is no longer restricted to the nucleus; (c) improved expression yield as soluble protein and improved stability in various hosts; (d) improved stability under oxidising conditions; (e) improved stability within cells after reaction with a substrate; (f) improved stability outside cells before and after reaction with a substrate; (g) improved in vitro solubility; (h) improved reactivity against O6-alkylguanine substrates; (1) reduced reactivity against DNA-based substrates; and (j) reduced reactivity against N9-substituted O6-alkylguanine substrates.
    Type: Grant
    Filed: March 1, 2005
    Date of Patent: February 15, 2011
    Assignee: Ecole Polytechnique Federale de Lausanne
    Inventors: Jan Barnikow, Christopher Chidley, Thomas Gronemeyer, Christian Heinis, Hughes Jaccard, Kai Johnsson, Alexandre Juillerat, Antje Keppler
  • Patent number: 7888081
    Abstract: Methods and systems for increasing the production of equilibrium reactions are described. In some embodiments, a method comprises removing enzymes from a reaction mixture once equilibrium is achieved, selectively re-adding enzymes and reactants for driving the product-forming reaction to product, and optionally recycling stabilized intermediates, by-products and/or co-products back into the reaction mixture. In some embodiments, a method comprises purifying the desired product from a reaction mixture and selectively recycling one or more components of the reaction mixture, such as original reactants, intermediates, co-products or by-products back into the reaction mixture as desired to improve titer. Systems for implementing the methods are also provided. In some embodiments the methods and systems are implemented for increasing the production of monatin and monatin derivatives, produced in multi-step equilibrium pathways.
    Type: Grant
    Filed: May 23, 2007
    Date of Patent: February 15, 2011
    Assignee: Cargill, Incorporated
    Inventors: Anil Bhagwan Khare, Brent H. Hilbert, Christopher Solheid, Sara C. McFarlan, Fernando A. Sanchez-Riera, Kent Floy, Paula M. Hicks
  • Patent number: 7888104
    Abstract: The invention relates to a novel enzyme obtained from Bacillus agaradherens (DSM 9948), which can be regarded as cyclodextrin glucanotransferase (CGTase) and also as ?-amylase. The invention also relates to sufficiently similar proteins or derivatives that exhibit amylolytic and/or CGTase activity, to the corresponding nucleic acids and micro-organisms that produce said proteins and derivatives, to methods for the production of said proteins and to diverse uses of the same. In addition, said proteins can be further developed for other, predominantly technical purposes. The invention relates in particular to detergents and cleaning agents containing CGTase of this type, to methods for cleaning textiles or hard surfaces using CGTase of this type or corresponding agents and to the use thereof for cleaning textiles or hard surfaces.
    Type: Grant
    Filed: November 17, 2001
    Date of Patent: February 15, 2011
    Assignee: Henkel AG & Co. KGaA
    Inventors: Beatrix Kottwitz, Karl-Heinz Maurer, Roland Breves, Irmgard Schmidt, Angrit Weber, Angela Hellebrandt, Laura Polanyi-Bald
  • Patent number: 7888063
    Abstract: This invention provides compositions and methods for producing translational components that expand the number of genetically encoded amino acids in eukaryotic cells. The components include orthogonal tRNAs, orthogonal aminoacyl-tRNA synthetases, orthogonal pairs of tRNAs/synthetases and unnatural amino acids. Proteins and methods of producing proteins with unnatural amino acids in eukaryotic cells are also provided.
    Type: Grant
    Filed: April 16, 2004
    Date of Patent: February 15, 2011
    Assignee: The Scripps Research Institute
    Inventors: Alexander Deiters, T. Ashton Cropp, Jason W. Chin, J. Christopher Anderson, Peter G. Schultz
  • Publication number: 20110033391
    Abstract: This invention relates generally to enzymes, polynucleotides encoding the enzymes, the use of such polynucleotides and polypeptides and more specifically to enzymes having transferase activity, e.g., transaminase activity, e.g., d-amino-acid transferase activity, and/or oxidoreductase activity, e.g., dehydrogenase activity, e.g., damino-acid dehydrogenase activity, and/or catalyze the transfer of a chemical group, catalyze transamination, catalyze the reaction: D-alanine+2-oxoglutarate<=>pyruvate+D-glutamate, and/or catalyze an oxidation-reduction reaction, catalyze the removal of hydrogen atoms, and/or catalyze the reaction: D-amino acid+H2O+acceptor<=>a 2-oxo acid+NH3+reduced acceptor.
    Type: Application
    Filed: December 31, 2008
    Publication date: February 10, 2011
    Applicant: VERENIUM CORPORATION
    Inventors: David P. Weiner, Peter Luginbuhl, Analia Bueno, Joslin M. Cuenca, Erin Marasco
  • Publication number: 20110033873
    Abstract: The present invention relates to proteomic markers for early detection of hepatocellular carcinoma, compositions for detecting changes of these proteomic markers, kits for detection of hepatocellular carcinoma, methods for detecting proteomic markers including these compositions, methods for screening drugs for hepatocellular carcinoma using these proteomic markers, and antibodies specific for these proteomic markers.
    Type: Application
    Filed: March 12, 2009
    Publication date: February 10, 2011
    Inventors: Jin Young Park, Seok Joo Hong, Jongmin Kim, Youngtack Shim
  • Patent number: 7883881
    Abstract: The present invention relates to inhibitors of GSK-3 and methods for producing these inhibitors. The invention also provides pharmaceutical compositions comprising the inhibitors and methods of utilizing those compositions in the treatment and prevention of various disorders, such as diabetes and Alzheimer's disease. In addition, the invention relates to molecules or molecular complexes which comprise binding pockets of GSK-3? or its homologues. The invention relates to a computer comprising a data storage medium encoded with the structure coordinates of such binding pockets. The invention also relates to methods of using the structure coordinates to solve the structure of homologous proteins or protein complexes. The invention relates to methods of using the structure coordinates to screen for and design compounds that bind to GSK-3? protein or homologues thereof. The invention also relates to crystallizable compositions and crystals comprising GSK-3? protein or GSK-3? protein complexes.
    Type: Grant
    Filed: January 5, 2010
    Date of Patent: February 8, 2011
    Assignee: Vertex Pharmaceuticals Incorporated
    Inventors: Ernst ter Haar, Lovorka Swenson, Jeremy Green, Michael J. Arnost
  • Patent number: 7883866
    Abstract: Compositions and methods of producing components of protein biosynthetic machinery that include orthogonal tRNA's, orthogonal aminoacyl-tRNA synthetases, and orthogonal pairs of tRNA's/synthetases are provided. Methods for identifying these orthogonal pairs are also provided along with methods of producing proteins using these orthogonal pairs.
    Type: Grant
    Filed: March 15, 2010
    Date of Patent: February 8, 2011
    Assignee: Ambrx, Inc.
    Inventors: Andrew Paulsel, Ho Sung Cho
  • Patent number: 7883860
    Abstract: The present invention provides the identification of human Ras palmitoyl acyl transfersase complexes, and nucleic acids coding therefor. In addition, methods of screening for modulators of human Ras palmitoyl acyl transfersase, including high throughput yeast screens, are also provided.
    Type: Grant
    Filed: December 30, 2008
    Date of Patent: February 8, 2011
    Assignees: Washington University, The University of Iowa Research Foundation
    Inventors: Robert J. Deschenes, Sandra Lobo, John Swarthout, Maurine E. Linder
  • Publication number: 20110030098
    Abstract: The invention provides method for producing a plant cell or plant with increased phlorizin or phloretin glycosyltransferase activity, the method comprising transformation of a plant cell or plant with a polynucleotide encoding a polypeptide with phloretin glycosyltransferase activity. The invention also provides host cells, plant cells and plants, genetically modified to contain and or express the polynucleotides.
    Type: Application
    Filed: June 18, 2010
    Publication date: February 3, 2011
    Inventors: Helene Jugde, Ross Atkinson
  • Publication number: 20110027789
    Abstract: The present invention relates to methods and kits for preserving genomic DNA sequence complexity within chemically and/or enzymatically converted DNA by an enzyme or series of enzymes that adds a methyl group to a cytosine outside of CpG dinucleotide sequences of genomic DNA. Further, the present invention relates to methylation analysis of the genomic DNA.
    Type: Application
    Filed: August 2, 2010
    Publication date: February 3, 2011
    Applicant: EPIGENOMICS AG
    Inventor: Joern Lewin
  • Publication number: 20110027833
    Abstract: The present invention provides mutants of DNA polymerases having an increased rate of incorporation of nucleotides into nucleic acids undergoing polymerization and having an enhanced resistance to inhibitors of DNA polymerase activity. The mutant polymerases are well suited for fast PCR applications, for PCR amplification of targets in samples that contain inhibitors of wild-type polymerases, and for fast PCR amplification of samples containing DNA polymerase inhibitors. In exemplary embodiments, the mutants are mutants of Taq DNA polymerase.
    Type: Application
    Filed: August 2, 2010
    Publication date: February 3, 2011
    Inventors: Holly Hogrefe, Michelle Cayouette, Jeffrey Fox, Connie J. Hansen, Jennifer Lapira, Bahram Arezi, Nancy McKinney
  • Publication number: 20110028423
    Abstract: The present invention provides an agent that modulates physiological condition of pests, wherein the agent has an ability to modulate the activity of an insect choline acetyltransferase; a method for assaying pesticidal activity of a test substance, which comprises measuring the activity of a choline acetyltransferase in a reaction system in which the choline acetyltransferase contacts with a test substance, and the like.
    Type: Application
    Filed: November 21, 2008
    Publication date: February 3, 2011
    Applicant: SUMITOMO CHEMICAL COMPANY, LIMITED
    Inventors: Junko Otsuki, Marc Van De Craen, Annelies Roobrouck, Guy Nys, Bert Demey
  • Patent number: 7880057
    Abstract: The present invention relates to a new plant breeding process. The process improves the agronomic performance of crop plants by using genetic material that is also used in classical breeding. Instead of sexually recombining entire genomes at random, as is done in classical breeding, specific genetic elements are rearranged in vitro and inserted back into individual plant cells. Plants obtained through this new plant breeding process do not contain foreign nucleic acid but only contain nucleic acid from the plant species selected for transformation or plants that are sexually compatible with the selected plant species. Plants developed through this new plant breeding process are provided. In particular, potato plants displaying improved tuber storage and health characteristics are provided.
    Type: Grant
    Filed: January 31, 2007
    Date of Patent: February 1, 2011
    Assignee: J.R. Simplot Company
    Inventors: Caius Rommens, Kathy M. M. Swords
  • Patent number: 7879586
    Abstract: A gene encoding a methylated catechin synthase that can effectively synthesize methylated catechins having high antiallergic activity. The gene encoding a methylated catechin synthase contains at least one nucleotide sequence selected from the group consisting of SEQ ID NOs: 1, 3 and 5.
    Type: Grant
    Filed: November 14, 2005
    Date of Patent: February 1, 2011
    Assignees: Incorporated Administrative Agency National Agriculture and Food Research Organization, Asahi Breweries, Ltd.
    Inventors: Mari Yamamoto, Masanobu Kirita, Manabu Sami, Mitsuo Ikeda
  • Patent number: 7879591
    Abstract: Lysophosphatidic acid acyltransferase [“LPAAT”] participates in the second step of oil biosynthesis and is expected to play a key role in altering the quantity of long-chain polyunsaturated fatty acids [“LC-PUFAs”] produced in oils of oleaginous organisms. An LPAAT isolated from Mortierella alpina [“MaLPAAT1”] that is suitable for use in the manufacture of oils enriched in LC-PUFAs in oleaginous organisms is disclosed. Most desirably, the substrate specificity of the instant MaLPAAT1 will be particularly useful to enable increased C18 to C20 elongation conversion efficiency and increased ?4 desaturation conversion efficiency in recombinant host cells producing LC-PUFAs.
    Type: Grant
    Filed: June 16, 2009
    Date of Patent: February 1, 2011
    Assignee: E.I. du Pont de Nemours and Company
    Inventors: Howard Glenn Damude, Zhixiong Xue, Narendra S. Yadav, Quinn Qun Zhu
  • Patent number: 7880058
    Abstract: This invention pertains to nucleic acid fragments encoding plant proteins that are homologs to the cis-prenyltransferases UPP synthase from the bacterium Micrococcus luteus or Dedol-PP synthase from yeast Saccharomyces cerevisiae. More specifically, this invention pertains to cis-prenyltransferase homologs from wheat, grape, soybean, rice, African daisy, rubber tree latex and pot marigold.
    Type: Grant
    Filed: August 23, 2007
    Date of Patent: February 1, 2011
    Assignee: Pioneer Hi-Bred International, Inc.
    Inventors: David L Hallahan, Christopher Coldren, Dennis Flint, Hong Wang
  • Publication number: 20110020311
    Abstract: A novel etiological hypothesis for Multiple Sclerosis (MS) is proposed describing autoimmune attack of ATP: Cob (I) alamin adenosyltransferase (ATR) thereby inhibiting synthesis of (5?-deoxy-5?-adenosyl) cobamide (referred to as 5?-deoxyadenosylcobalmin or AdoCbl) from Vitamin B12 providing a basis for therapeutic design and diagnostic methods. Pharmaceutical compositions for therapy of MS, inflammatory neurological diseases and neurodegenerative diseases utilizing AdoCbl, growth hormone, immunomodulators, interleukins, other therapeutic agents, and physiotherapy are described. Encompassed in embodiments are diagnostic and therapeutic methods based on the amino acid sequence which binds AdoCbl in ATR. A scoping experiment in therapy, parenteral injection of AdoCbl, has been accomplished with a clinically definite MS patient in the Secondary Progressive stage of MS. The dramatic improvement in the patient's condition strongly supports the etiological hypothesis.
    Type: Application
    Filed: July 27, 2009
    Publication date: January 27, 2011
    Inventor: James Layne Boucher
  • Publication number: 20110023194
    Abstract: The ADP-ribosyltransferase, Vip2, exerts its intracellular toxicity in insects by modifying actin and preventing actin polymerization. Due to the nature of this toxin, expression of Vip2 in planta is lethal to the plant. Described herein are methods of making zymogens of toxic proteins that are benign in a non-target organism and are activated in a target organism. Disclosed herein are methods of engineering a random propeptide library at a terminus of a toxic protein and selecting for malfunctional variants in yeast. Using this method a selected proenzyme possesses reduced enzymatic activity as compared to the wild-type Vip2 protein, but remains a potent toxin towards corn rootworm larvae. The Vip2 zymogen can be proteolytically activated by corn rootworm digestive proteases.
    Type: Application
    Filed: December 10, 2008
    Publication date: January 27, 2011
    Applicant: SYNGENTA PARTICIPATIONS AG
    Inventors: Jucovic Milan, Jeng Shong Chen, Narenka V. Palekar, Frederick S. Walters
  • Publication number: 20110021610
    Abstract: Nucleic acid molecules from hop (Humulus lupulus) have been isolated and characterized wherein said nucleic acid molecules encode polypeptides having aromatic prenyltransferase activity Expression or over-expression of said nucleic acid molecules alters the level of terpenophenolic compounds The polypeptides may be used in vivo or in vitro to produce terpenophenolic compounds (e g, prenylated acylphloroglucmols and prenylflavonoids) such as prenyl-PIVP, prenyl-PIBP, humulone, lupulone, desmethylxanthohumol and xanthohumol
    Type: Application
    Filed: March 16, 2009
    Publication date: January 27, 2011
    Applicant: NATIONAL RESEARCH COUNCIL OF CANADA
    Inventors: Jonathan Page, Enwu Liu, Jana Nagel
  • Publication number: 20110014679
    Abstract: A DNA polymerase reaction system which provides high DNA polymerase activity even at a high temperature and at a high salt concentration. A DNA polymerase reaction system that is constructed from a DNA polymerase, a clamp, and a clamp loader without intein sequence, the DNA polymerase being from Pyrococcus horikoshii, a hyperthermophilic archaeon.
    Type: Application
    Filed: August 25, 2010
    Publication date: January 20, 2011
    Applicant: National Inst. of Adv Industrial Science and Tech.
    Inventors: Ikuo MATSUI, Yuji Urushibata
  • Publication number: 20110014678
    Abstract: Isolated sulfotransferase nucleic acid molecules that include a nucleotide sequence variant and nucleotides flanking the sequence variant are described, as well as sulfotransferase allozymes. Methods for determining if a mammal is predisposed to cancer also are described.
    Type: Application
    Filed: August 18, 2010
    Publication date: January 20, 2011
    Applicant: MAYO FOUNDATION FOR MEDICAL EDUCATION AND RESEARCH
    Inventors: Araba A. Adjei, Eric D. Wieben, Richard M. Weinshilboum, Bianca A. Thomae
  • Publication number: 20110014677
    Abstract: A DNA fragment containing a gene which encodes a specific gene regulatory region alone or the gene regulatory region together with a signal peptide; a recombinant vector containing the DNA fragment; a transformant containing the recombinant vector; and a method of producing a recombinant protein by using the transformant. According to the invention, it is possible to produce a protein in a large amount at a high efficiency regardless of the kind of the recombinant protein.
    Type: Application
    Filed: July 9, 2008
    Publication date: January 20, 2011
    Inventors: Yuji Hatada, Yukari Ohta, Yuko Hidaka, Nobuyuki Nakamura
  • Publication number: 20110016585
    Abstract: The present disclosure relates to isolated polynucleotides encoding a delta 9-elongase, delta 9-elongases encoded by the isolated polynucleotides, expression vectors comprising the isolated polynucleotides, host cells comprising the expression vectors, and methods for producing delta 9-elongase and polyunsaturated fatty acids.
    Type: Application
    Filed: July 17, 2009
    Publication date: January 20, 2011
    Applicant: ABBOTT LABORATORIES
    Inventors: Suzette Pereira, Tapas Das, Padmavathy Krishnan, Pradip Mukerji
  • Publication number: 20110014196
    Abstract: The invention relates to compounds comprising a plurality of enzyme substrates suitably linked and further carrying one or more cargo entities. In particular such compounds have the structure (substrate)n-linker-(cargo)m wherein “substrate” is a substrate specific for an enzyme-type protein; n is 2 or more; “linker” is a linking unit consisting of 1 to 300 atoms; “cargo” is a drug, a label detectable by a fluorescence detector, magnetic resonance imaging (MRI), positron emission tomography (PET) or scintigraphy, or a functional group which can be transformed into a drug or a detectable label; and m is 1 or more. The invention further relates to a corresponding molecular shuttles having the structure (fusion protein)n-linker-(cargo)m wherein “fusion protein” is a proteinaceous binding entity fused to an enzyme-type protein for which specific substrates exist. The proteinaceous binding entity is designed to bind to a target structure in vitro or in vivo, for example a cellular receptor.
    Type: Application
    Filed: October 2, 2008
    Publication date: January 20, 2011
    Applicant: COVALYS BIOSCIENCES AG
    Inventors: Andreas Brecht, Florent Beaufils
  • Publication number: 20110014223
    Abstract: The present invention relates to the fields of molecular biology, immunology and medicine. Peptide sequences that belong to the beta amyloid peptide are exposed on the surface of a multimeric structure derived from the E2 component of alpha keto acid dehydrogenase. The molecules are useful for the production of vaccines for the treatment and/or prevention of Alzheimer's disease.
    Type: Application
    Filed: January 27, 2009
    Publication date: January 20, 2011
    Applicant: CONSIGLIO NAZIONALE DELLE RICERCHE
    Inventors: Antonella Prisco, Piergiuseppe De Berardinis, Diana De Falco, Antonella Caivano
  • Patent number: 7872111
    Abstract: A protein participating in the addition of mannose phosphate to a sugar chain of a glycoprotein originating in a yeast belonging to the genus Pichia; a gene coding this protein; a mutant of this gene; a vector carrying the mutant gene; a yeast strain belonging to the genus Pichia having been transformed by this vector; a process for producing a protein with reduction of an acidic sugar chain by using the transformed yeast strain; and a glycoprotein thus produced, are described.
    Type: Grant
    Filed: August 1, 2008
    Date of Patent: January 18, 2011
    Assignee: Mitsubishi Tanabe Pharma Corporation
    Inventors: Masami Miura, Masaaki Hirose, Taeko Miwa, Hiroyuki Irie, Shinobu Kuwae, Kenmi Miyano, Wataru Otani, Hideyuki Ohi
  • Patent number: 7871804
    Abstract: The invention relates to a method for producing polyunsaturated fatty acids in an organism, according to which nucleic acids coding for polypeptides with an acyl-CoA:lysophospholipid-acyltransferase activity are introduced into the organism. Advantageously, the nucleic acid sequences can be expressed in the transgenic organism optionally together with other nucleic acid sequences coding for polypeptides of the fatty acid or lipid metabolism. The invention also relates to the inventive nucleic acid sequences, nucleic acid constructs containing the inventive nucleic acid sequences, vectors containing the inventive nucleic acid sequences and/or the nucleic acid constructs, and transgenic organisms containing the nucleic acid sequences, nucleic acid constructs and/or vectors. The invention further relates to oils, lipids and/or fatty acids produced according to the inventive method, and to the use of the same.
    Type: Grant
    Filed: December 20, 2005
    Date of Patent: January 18, 2011
    Assignee: BASF Plant Science GmbH
    Inventors: Petra Cirpus, Jörg Bauer, Ernst Heinz, Amine Abbadi, Jelena Kirsch
  • Patent number: 7871805
    Abstract: The present invention provides glucose dehydrogenase which is excellent in heat resistance and substrate specificity and is not affected by dissolved oxygen. Specifically, the present invention relates to glucose dehydrogenase characterized by being derived from an eukaryotic organism and keeping 90% or more activity after being treated with heat at 55° C. for 15 minutes in a liquid form compared with the activity before being treated.
    Type: Grant
    Filed: January 9, 2009
    Date of Patent: January 18, 2011
    Assignee: Toyo Boseki Kabushiki Kaisha
    Inventors: Hiroshi Aiba, Hiroshi Kawaminami, Takahide Kishimoto, Yoshiaki Nishiya
  • Publication number: 20110008844
    Abstract: A sialyltransferase having the following physico-chemical properties: (1) Activity: transfers Sialic acid from a sialic acid donor selectively to a 3-hydroxyl group of a galactose residue contained in lactosylceramide as a sialic acid acceptor to produce ganglioside GM3; (2) Optimal Reaction pH: 6.0 to 7.0; and (3) Inhibition and Activation: the activity increases at least 1.5 times with 10 mM of Mn2+ as compared with the case in the absence thereof.
    Type: Application
    Filed: July 22, 2010
    Publication date: January 13, 2011
    Inventor: Masaki Saito
  • Publication number: 20110008795
    Abstract: Disclosed is a polynucleotide marker or a protein marker for use in the specific detection of an IL-17-producing helper T-cell (a Th17 cell). Also disclosed is a method for detecting a Th17 cell, which is characterized by detecting the occurrence of the polynucleotide marker or the protein marker.
    Type: Application
    Filed: February 27, 2009
    Publication date: January 13, 2011
    Applicant: SYSMEX CORPORATION
    Inventors: Masafumi Ikeda, Hitoshi Uga, Satoshi Tanaka, Yoshiaki Miyamoto, Masatoshi Yanagida, Hirokazu Kurata, Masakazu Kadowaki