Transferase Other Than Ribonuclease (2.) Patents (Class 435/193)
-
Publication number: 20110143399Abstract: The present invention provides a method to directionally clone any linear template DNA molecule into any linearized vector. The vector ends may be generated from any restriction enzyme cleavage. The method does not require a ligation step nor the use of carefully controlled conditions as is required with methods involving specific exonucleases alone. It has been determined that specific DNA polymerases are able to efficiently join one or more linear DNA molecules sharing ends with appropriate complementation.Type: ApplicationFiled: July 15, 2009Publication date: June 16, 2011Applicant: University of GuelphInventors: David Evans, David O. Willer, Xiao-Dan Yao
-
Publication number: 20110144317Abstract: Method for preparing the disaccharide [?-D-Gldp(1?3)]-?-L-Rhap-YR wherein Y is selected from —O— and —S— and R is selected from the group consisting of: C1-C6 alkyl, C1-C6 alkenyl, aryl, allyl, —CO-alkyl (C1-C6), —CO-alkenyl (C1-C6), —CO-aryl comprising the step of using a mutant of a wild type glycoside hydrolase.Type: ApplicationFiled: March 12, 2009Publication date: June 16, 2011Inventors: Laurence Mulard, Isabelle Andre, Elise Champion, Claire Moulis, Sandrine Morel, Pierre Monsan, Magali Remaud-Simeon, Julien Boutet
-
Publication number: 20110143418Abstract: Orthogonal ribosome orthogonal mRNA pairs are provided, as are methods for their selection involving a novel positive-negative selection approach, and methods for their use. Also provided are cellular logic circuits involving orthogonal ribosomes.Type: ApplicationFiled: December 8, 2010Publication date: June 16, 2011Inventors: OLIVER RACKHAM, Jason W. Chin
-
Publication number: 20110136203Abstract: The invention is directed to a method and kit to control and modify the status of cells, such as human stem cells, by changing their glycosylation, in particular sialylation and fucosylation, levels in a reaction condition where culture medium reagents, such as divalent cations, are present and cells are kept non-adherent. The invention is further directed to novel stem cells, the glycosylation of which has been specifically altered.Type: ApplicationFiled: July 16, 2009Publication date: June 9, 2011Applicants: SUOMEN PUNAINEN RISTI, VERIPALVELU, GLYKOS FINLAND LTDInventors: Johanna Nystedt, Heidi Anderson, Leena Valmu, Jari Natunen, Tero Satomaa
-
Publication number: 20110136178Abstract: The invention relates to a method for the amplification of nucleic acid sequences by enzymatic DNA polymerase activity, wherein synthetic oligodeoxynucleotide compounds are used as primers, comprising sequences having the formula (I), wherein the sequence SeqA-Ni-SeqC has at least a length of 8 nucleotides and can hybridize with one of the nucleic acid sequences to be amplified, SeqA and SeqC are sequences of naturally occurring deoxyribonucleotide building blocks or the synthetic analogs thereof, N1 is a phosphoribonucleotide group covalently bound to R1 or a nucleotide analog covalently bound to R1, R1 is a molecule group, comprising at least ten atoms having an atomic weight of twelve and higher, and to reaction mixtures for carrying out the method.Type: ApplicationFiled: January 9, 2009Publication date: June 9, 2011Inventor: Olfert Landt
-
Patent number: 7955813Abstract: A method for the in situ production of an emulsifier in a foodstuff, wherein a lipid acyltransferase is added to the foodstuff. Preferably the emulsifier is produced without an increase or without a substantial increase in the free fatty acid content of the foodstuff. Preferably, the lipid acyltransferase is one which is capable of transferring an acyl group from a lipid to one or more of the following acyl acceptors: a sterol, a stanol, a carbohydrate, a protein or a sub-unit thereof, glycerol. Preferably, in addition to an emulsifier one or more of a stanol ester or a stanol ester or a protein ester or a carbohydrate ester or a diglyceride or a monoglyceride may be produced. One or more of these may function as an additional emulsifier.Type: GrantFiled: July 7, 2006Date of Patent: June 7, 2011Assignee: Danisco, A/SInventors: Arno De Kreij, Susan Mampusti Madrid, Jørn Dalgaard Mikkelsen, Jørn Borch Søe
-
Patent number: 7955830Abstract: The present invention relates to isolated polypeptides having lipase activity and isolated polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods for producing and using the polypeptides.Type: GrantFiled: November 11, 2010Date of Patent: June 7, 2011Assignees: Novozymes, Inc., Novozymes A/SInventors: Debbie Yaver, Suzanne Otani, Janine Lin, Christopher Amolo, Kim Borch, Shamkant Anant Patkar, Michael Lamsa, Barbara Cherry
-
Patent number: 7955814Abstract: A method for the in situ production of an emulsifier in a foodstuff, wherein a lipid acyltransferase is added to the foodstuff. Preferably the emulsifier is produced without an increase or without a substantial increase in the free fatty acid content of the foodstuff. Preferably, the lipid acyltransferase is one which is capable of transferring an acyl group from a lipid to one or more of the following acyl acceptors: a sterol, a stanol, a carbohydrate, a protein or a sub-unit thereof, glycerol. Preferably, in addition to an emulsifier one or more of a stanol ester or a stanol ester or a protein ester or a carbohydrate ester or a diglyceride or a monoglyceride may be produced. One or more of these may function as an additional emulsifier.Type: GrantFiled: February 6, 2007Date of Patent: June 7, 2011Assignee: Danisco A/SInventors: Arno De Kreij, Susan Mampusti Madrid, Jørn Dalgaard Mikkelsen, Jørn Borch Søe, Mark Turner, Jonathan Goodwins
-
Publication number: 20110129879Abstract: Disclosed are hybrid genes of glucanase and dextransucrase, recombinant vectors comprising said hybrid genes, microorganisms which are transformed with said recombinant vectors, hybrid enzymes which are expressed from said hybrid genes, and processes for preparing isomalto-oligosaccharides or dextran using said microorganisms or enzymes. Expensive isomalto-oligosaccharides and low molecular weight dextran for clinical use can be produced simply and effectively from cheap substrate-sucrose, using a single bacterial strain or enzyme.Type: ApplicationFiled: April 28, 2007Publication date: June 2, 2011Applicant: INDUSTRY FOUNDATION OF CHONNAM NATIONAL UNIVERSITYInventors: Do Man Kim, Mi Young Seo
-
Publication number: 20110129895Abstract: A method is provided for purifying protein having steps of loading a sample containing proteins into a column containing an ion exchanger in a first direction to allow the ion exchanger to absorb proteins; and passing a salt solution through the column in a second direction opposite to the first direction to elute a target protein from the ion exchanger. The method is proven as capable of improving homogeneity of eluted target protein without combining minute and complicated techniques for protein purification. Therefore, the method is convenient, efficient and economic for purifying proteins.Type: ApplicationFiled: May 25, 2010Publication date: June 2, 2011Applicant: Tamkang UniversityInventors: Ming-Kai Chern, Wei-Jhy Shiah, Jyun-Jie Chen, Tzung-You Tsai, Hsin-Yin Lin, Chien-Wei Liu
-
Publication number: 20110126318Abstract: This invention relates to polynucleotide sequences encoding SUT2 or SUT4 sucrose transporter genes. Methods for increasing seed oil content and evaluating increased oil content in a plant seed are described. The compositions and methods disclosed herein employ a variety of sequences that encode sucrose transporters and a variety of sequences that influence fatty acid accumulation, including for example, DGAT, Lec1 and ODP1 transcription factor. In specific embodiments, overexpression of SUT2 and/or SUT4 sucrose transporters in combination with DGAT genes further increase plant seed oil production compared to a high oil plant comprising recombinant DNA constructs that do not overexpress SUT2 or SUT4 transporters.Type: ApplicationFiled: November 1, 2010Publication date: May 26, 2011Applicant: E.I. duPont de Nemours and CompanyInventors: Stephen M. Allen, Howard Glenn Damude, John D. Everard, Knut Meyer, Byung-Chun Yoo
-
Publication number: 20110124082Abstract: To obtain a mutant protein of an asparagine-linked glycoprotein, which has no N-linked sugar chain under ordinary circumstance, and remains a physiological activity of the glycoprotein before the mutation was introduced, at least one of the amino acids contained in the amino acid sequence motif (I) and/or (II) in the polypeptide of the asparagine-linked glycoprotein is substituted into another amino acid: (I) Asn Xa1 Xa2 (II) Xa3 Val Gly Asn Xa1 Xa2. In amino acid sequence motif (I) and (II), Xa1 represents an amino acid other than Pro, Xa2 represents Thr or Ser and Xa3 represents His or Asp.Type: ApplicationFiled: November 10, 2010Publication date: May 26, 2011Applicant: Seikagaku CorporationInventors: Jin-ichi Inokuchi, Satoshi Uemura
-
Patent number: 7947481Abstract: A vector of the present invention has DNA encoding a protein or a product having the same effect as the protein, the protein containing an amino acid sequence from amino acid numbers 47 to 802 in SEQ. ID. NO:2. Expression of the DNA gives human chondroitin synthase. By using human chondroitin synthase, it is possible to produce a saccharide chain having a repeating disaccharide unit of chondroitin. The DNA or part thereof may be used as a probe for hybridization for the human chondroitin synthase.Type: GrantFiled: January 30, 2008Date of Patent: May 24, 2011Assignee: Otsuka Chemical Co., Ltd.Inventors: Kazuyuki Sugahara, Hiroshi Kitagawa
-
Publication number: 20110118434Abstract: The present invention relates to a method for producing and preparing polyglycolate (PGA) from genetically engineered organisms. More specifically, the invention relates to a method comprising two steps; 1) culturing, in a medium containing glycolic acid or not, the microorganism expressing at least one gene encoding an enzyme(s) that converts glycolate into glycolyl-CoA, and a gene encoding polyhydroxyalkanoate (PHA) synthase which uses glycolyl-CoA as a substrate, 2) recovering the polyglycolate polymer.Type: ApplicationFiled: July 10, 2009Publication date: May 19, 2011Inventors: Philippe Soucaille, Wanda Dischert
-
Publication number: 20110117608Abstract: The invention is directed towards constructs for RNAi techniques. The invention provides a ribonucleic acid (RNA) for use as interfering RNA in gene silencing techniques to silence a target gene comprising in a 5? to 3? direction at least a first effector sequence, a second effector sequence, a sequence substantially complementary to the second effector sequence and a sequence substantially complementary to the first effector sequence, wherein the complementary sequences are capable of forming double stranded regions with their respective effector sequences and wherein at least one of these sequences is substantially identical to the predicted transcript of a region of the target gene, and a nucleic acid construct encoding such an RNA.Type: ApplicationFiled: October 28, 2010Publication date: May 19, 2011Applicant: BENITEC AUSTRALIA, LTD.Inventors: MICHAEL WAYNE GRAHAM, KENNETH CLIFFORD REED, ROBERT NORMAN RICE, BRUCE THOMAS HARRISON, PETRUS ROELVINK, DAVID SUHY, ALEXANDER KOLYKHALOV
-
Publication number: 20110119787Abstract: This invention relates to isolated nucleic acid fragments encoding fructosyltransferases. More specifically, this invention relates to polynucleotides encoding 1-FFTs, 6-SFTs, or 1-SSTs. The invention also relates to the construction of a recombinant DNA constructs encoding all or a portion of the fructosyltransferases, in sense or antisense orientation, wherein expression of the recombinant DNA construct results in production of altered levels of the fructosyltransferases in a transformed host cell.Type: ApplicationFiled: January 21, 2011Publication date: May 19, 2011Applicant: E.I. DU PONT DE NEMOURS AND COMPANYInventors: JOHAN M. STOOP, Stephen M. Allen, Perry G. Caimi, Karlene H. Butler
-
Patent number: 7943355Abstract: Isolated sulfotransferase nucleic acid molecules that include a nucleotide sequence variant and nucleotides flanking the sequence variant are described, as well as sulfotransferase allozymes. Methods for determining if a mammal is predisposed to cancer also are described.Type: GrantFiled: August 18, 2010Date of Patent: May 17, 2011Assignee: Mayo Foundation for Medical Education and ResearchInventors: Araba A Adjei, Eric D Wieben, Richard M Weinshilboum, Bianca A Thomae
-
Publication number: 20110110914Abstract: Methods and formulations for treating oncological disorders in humans using epimetabolic shifters, multidimensional intracellular molecules or environmental influencers are described.Type: ApplicationFiled: May 11, 2010Publication date: May 12, 2011Inventors: Niven Rajin Narain, John Patrick McCook, Rangaprasad Sarangarajan
-
Publication number: 20110111468Abstract: The present disclosure provides acyltransferases useful for synthesizing therapeutically important statin compoundType: ApplicationFiled: September 24, 2010Publication date: May 12, 2011Applicant: Codexis, Inc.Inventors: Lynne GILSON, Steven James COLLIER, Joly SUKUMARAN, Wan Lin YEO, Oscar ALVIZO, Ee Ling TEO, Robert John WILSON, Junye XU
-
Publication number: 20110111454Abstract: CgtB proteins with enhanced beta 1,3-galactosaminyltransferase activity, nucleic acids that encode the CgtB proteins and methods for use of the CgtB proteins.Type: ApplicationFiled: April 18, 2008Publication date: May 12, 2011Applicant: National Research Council of CanadaInventors: Stephane Bernatchez, Michel Gilbert, Warren Wakarchuk
-
Patent number: 7939301Abstract: The present invention is directed towards an asymmetric enzymic process for preparing optically active organic compounds. The process according to the invention is carried out in what are termed miniemulsions. The invention also relates to an enzymic reaction mixture which exhibits a miniemulsion.Type: GrantFiled: November 4, 2005Date of Patent: May 10, 2011Assignee: Evonik Degussa GmbHInventors: Harald Gröger, Karlheinz Drauz, Hendrik Hüsken, Katharina Landfester
-
Patent number: 7939305Abstract: The invention relates to genes which are coded for sequences specific to polyketide synthases (PKS). The thus synthesized PKS is characterized by the enzymatic capacity thereof to produce PUFAs (polyunsaturated fatty acids). The invention also relates to the identification of the corresponding DNA-sequences, in addition to the use of said nucleotide sequences for the production of recombined and/or transgenic organisms.Type: GrantFiled: April 8, 2005Date of Patent: May 10, 2011Assignee: Nutrinova Nutrition Specialties & Food Ingredients GmbHInventors: Markus Luy, Matthias Rüsing, Thomas Kiy
-
Patent number: 7939065Abstract: The invention provides a chondroitin sulfate synthesis promoter useful for the treatment of diseases such as articular disease and discopathy. The chondroitin sulfate synthesis promoter contains, as an active ingredient, chondroitin sulfate glucuronyltransferase protein and/or chondroitin sulfate N-acetylgalactosaminyltransferase-1 protein, or a gene encoding the enzyme protein(s).Type: GrantFiled: November 18, 2008Date of Patent: May 10, 2011Assignees: Tokyo Medical and Dental University, Seikagaku CorporationInventors: Kenichiro Sakai, Koji Kimata, Kenichi Shinomiya, Hideto Watanabe
-
Publication number: 20110104138Abstract: A method to treat a disease, disorder or condition caused by a negative-sense single-strand RNA virus in an individual in need, comprising at least the step of administering to said individual in need, an isolated 2?-5?-oligoadenylate synthetase like protein or an isolated polynucleotide encoding said 2?-5?-oligoadenylate synthetase like protein.Type: ApplicationFiled: November 3, 2009Publication date: May 5, 2011Applicant: INSTITUT PASTEURInventors: Jean-Jacques Raoul Panthier, Tania Zaverucha Do Valle, Agnes Marie Monique Billecocq, Michele Bouloy, Xavier Bernard Philippe Montagutelli
-
Publication number: 20110107455Abstract: The subject invention relates to a novel gene referred to herein as DSM-2. This gene was identified in Sterptomyces coelicolor A3. The DSM-2 protein is distantly related to PAT and BAR. The subject invention also provides plant-optimized genes encoding DSM-2 proteins, DSM-2 can be used as a transgenic trait to impart tolerance in plants and plant cells to the herbicides glufosinate and bialaphos. One preferred use of the subject genes are as selectable markers. The use of this gene as a selectable marker in a bacterial system can increase efficiency for plant transformations. Use of DSM-2 as the sole selection marker eliminates the need for an additional medicinal antibiotic marker (such as ampicillin resistance) during cloning. Various other uses are also possible according to the subject invention.Type: ApplicationFiled: December 7, 2007Publication date: May 5, 2011Applicant: Dow AgroSciences LLCInventors: Justin M. Lira, Terry R. Wright, Sean M. Russell, Donald J. Merlo, Steven Robert Webb, Nicole L. Arnold, Andrew E. Robinson, Kelley A. Smith
-
Publication number: 20110104784Abstract: In one aspect, the present invention provides methods for storing a composition useful for synthesizing nucleic acid molecules. The methods of this aspect of the invention include the steps of: (a) freezing multiple aliquots of a liquid composition comprising from 1000 units/mL to 5000 units/mL of a reverse transcriptase, or from 10,000 units/mL to 50,000 units/mL of an RNA polymerase, wherein the multiple aliquots of the liquid composition are disposed within multiple receptacles defined by a container body; and (b) a step selected from the group consisting of (1) storing the frozen aliquots at a temperature below ?15° C., and (2) drying the frozen aliquots to produce dried aliquots of the composition, wherein each dried aliquot of the composition comprises an amount of water that is less than 0.1% by weight of the dried aliquot, and storing the dried aliquots at a temperature below ?15° C.Type: ApplicationFiled: October 15, 2010Publication date: May 5, 2011Applicant: LIFE TECHNOLOGIES CORPORATIONInventor: Mingjie ZHOU
-
Publication number: 20110097765Abstract: Described herein are compositions and methods for enhanced fermentation of molasses using transglucosidase.Type: ApplicationFiled: April 30, 2009Publication date: April 28, 2011Inventors: Gang Duan, Zhenhua Ruan, Jayarama K. Shetty, Hongxian Xu
-
Patent number: 7932077Abstract: Engineered strains of the oleaginous yeast Yarrowia lipolytica capable of producing greater than 25% eicosapentaenoic acid (EPA, an ?-3 polyunsaturated fatty acid) in the total oil fraction are described. These strains comprise various chimeric genes expressing heterologous desaturases, elongases and acyltransferases and optionally comprise various native desaturase and acyltransferase knockouts to enable synthesis and high accumulation of EPA. Production host cells are claimed, as are methods for producing EPA within said host cells.Type: GrantFiled: November 2, 2005Date of Patent: April 26, 2011Assignee: E. I. du Pont de Nemours and CompanyInventors: Howard Glenn Damude, Peter John Gillies, Daniel Joseph Macool, Stephen K. Picataggio, Dana M. Walters Pollak, James John Ragghianti, Zhixiong Xue, Narendra S. Yadav, Hongxiang Zhang, Quinn Qun Zhu
-
Publication number: 20110091952Abstract: Disclosed herein are methods of preparing alkenes from beta-hydroxy or beta-sulfate carboxylic acid or carboxylic acid derivatives using thioesterase and optionally a sulfotransferase.Type: ApplicationFiled: July 23, 2010Publication date: April 21, 2011Applicant: THE REGENTS OF THE UNIVERSITY OF MICHIGANInventors: David H. Sherman, Janet L. Smith, Liangcai Gu, Jennifer Gehret
-
Publication number: 20110093983Abstract: The present disclosure relates to the isolation of, purification of, characterization of, and uses for a glycerol-3-phosphate acyltransferase (TpGPAT1), and genes encoding TpGPAT1, from algae.Type: ApplicationFiled: March 26, 2009Publication date: April 21, 2011Applicant: National Research Council of CanadaInventors: Jitao Zou, Zhifu Zheng, Jingyu Xu
-
Patent number: 7927853Abstract: It is intended to obtain DNA ligase improved in binding ability and reactivity with DNA, particularly thermostable DNA ligase improved in binding ability and reactivity with DNA. The present invention provides a DNA ligase mutant improved in binding ability and reactivity with DNA, which is obtained by partially or completely deleting a C-terminal helix portion of DNA ligase. Particularly, the mutant is derived from a thermostable bacterium.Type: GrantFiled: June 23, 2006Date of Patent: April 19, 2011Assignee: Hitachi, Ltd.Inventors: Hirokazu Nishida, Yoshizumi Ishino, Kosuke Morikawa
-
Patent number: 7927601Abstract: The present invention relates generally to viral variants exhibiting reduced sensitivity to particular agents and/or reduced interactivity with immunological reagents. More particularly, the present invention is directed to hepatitis B virus (HBV) variants exhibiting complete or partial resistance to nucleoside or nucleotide analogs or other antagonists of HBV DNA polymerase activity and/or reduced interactivity with antibodies to viral surface components including reduced sensitivity to these antibodies. The present invention further contemplates assays for detecting such viral variants. These assays are useful in monitoring anti-viral therapeutic regimens and in developing new or modified vaccines directed against viral agents and in particular the resistant HBV variants of the present invention. The present invention also contemplates the use of the viral variants to screen for agents capable of inhibiting infection, replication and/or release of the virus.Type: GrantFiled: September 28, 2005Date of Patent: April 19, 2011Assignees: Melbourne Health, Fundacion Investigacion y Educacion En SidaInventors: Julie Sheldon, Berta Rodes, Vincent Soriano, Angeline Ingrid Bartholomeusz
-
Patent number: 7927837Abstract: Disclosed are: (A) a polypeptide consisting of the amino acid sequence of SEQ ID NO:2, or (B) a polypeptide comprising an amino acid sequence of SEQ ID NO:2 including deletion, substitution or addition of one or several amino acid residues and having chondroitin synthase activity; a nucleic acid encoding the polypeptide; a method for producing the polypeptide, comprising at least the steps of: (1) expressing the nucleic acid to produce the polypeptide; and (2) collecting the polypeptide produced in the step (1); and a crystal of the polypeptide. The crystal may be a monoclinic or tetragonal crystal.Type: GrantFiled: June 12, 2007Date of Patent: April 19, 2011Assignee: Seikagaku CorporationInventors: Yoshimitsu Kakuta, Takuo Osawa, Nobuo Sugiura, Koji Kimata
-
Patent number: 7927859Abstract: The invention relates to increasing the yield of succinate in bacteria by increasing the intracellular availability of cofactors such as NADH.Type: GrantFiled: August 20, 2004Date of Patent: April 19, 2011Assignee: Rice UniversityInventors: Ka-Yiu San, George N. Bennett, Ailen Sánchez
-
Publication number: 20110086013Abstract: The present invention is directed to a polynucleotide sequence of a novel acylglycerol acyltransferase-like protein MGAT-X1. The invention also provides the human MGAT-X1 associated with the dermatological diseases, urological diseases, muscle-skeleton disorders, hematological diseases, cancer, reproduction disorders, neurological diseases, metabolic diseases, cardiovascular diseases or gastroenterological diseases. The invention also provides assays for the identification of compounds useful for the modulation of dermatological diseases, urological diseases, muscle-skeleton disorders, hematological diseases, cancer, reproduction disorders, neurological diseases, metabolic diseases, cardiovascular diseases or gastroenterological diseases for treating of such diseases associated with expression of the MGAT-X1. The invention also features compounds which bind to and/or activate or inhibit the activity of MGAT-X1 as well as pharmaceutical compositions comprising such compounds.Type: ApplicationFiled: August 31, 2010Publication date: April 14, 2011Applicant: BAYER SCHERING PHARMA AGInventors: Stefan Golz, Ulf Brüggemeier, Bernhard Weingärtner, Andreas Geerts
-
Publication number: 20110086407Abstract: The present invention relates to an isolated polynucleotide of the complete chromosome of Bacillus licheniformis. The present invention also relates to isolated genes of the chromosome of Bacillus licheniformis which encode biologically active substances and to nucleic acid constructs, vectors, and host cells comprising the genes as well as methods for producing biologically active substances encoded by the genes and to methods of using the isolated genes of the complete chromosome of Bacillus licheniformis.Type: ApplicationFiled: December 17, 2010Publication date: April 14, 2011Applicants: Novozymes A/S, Novozymes, Inc.Inventors: Randy Berka, Michael Rey, Preethi Ramaiya, Jens Tønne Andersen, Michael Dolberg Rasmussen, Peter Bjarke Olsen
-
Patent number: 7923225Abstract: The present invention relates to a method for the enzymatic production of 2-hydroxy-2-methyl carboxylic acids from 3-hydroxy carboxylic acids, where a 3-hydroxy carboxylic acid is produced in an aqueous reaction solution and/or is added to this reaction solution and is incubated. The aqueous reaction solution comprises a unit having 3-hydroxy-carboxylate-CoA mutase activity which has both 3-hydroxy-carbonyl-CoA ester-producing and 3-hydroxy-carbonyl-CoA ester-isomerizing activity and converts the 3-hydroxy carboxylic acid into the corresponding 2-hydroxy-2-methyl carboxylic acid which is isolated as acid or in the form of its salts. In a preferred embodiment, the unit having 3-hydroxy-carboxylate-CoA mutase activity is a unit which includes an isolated cobalamin-dependent mutase and where appropriate a 3-hydroxy-carbonyl-CoA ester-producing enzyme or enzyme system or a microorganism including them.Type: GrantFiled: March 23, 2007Date of Patent: April 12, 2011Assignee: Evonik Roehm GmbHInventors: Roland H. Mueller, Thore Rohwerder
-
Patent number: 7923600Abstract: The present invention provides a convenient method for producing a transformed plant by expressing a hormone gene positioned within a plasmid backbone that also carries a P-DNA or T-DNA to obtain backbone-free transformed plants.Type: GrantFiled: February 2, 2007Date of Patent: April 12, 2011Assignee: J.R. Simplot CompanyInventor: Craig Richael
-
Publication number: 20110081703Abstract: Provided is a chimeric isoprenoid synthase polypeptide including a first domain from a first isoprenoid synthase joined to a second domain from a second, heterologous, isoprenoid synthase, whereby the chimeric isoprenoid synthase is capable of catalyzing the production of isoprenoid reaction products that are not produced in the absence of the second domain of the second, heterologous, isoprenoid synthase. Also provided is a chimeric isoprenoid synthase polypeptide including an asymmetrically positioned heterologous domain, whereby the chimeric isoprenoid synthase is capable of catalyzing the production of isoprenoid reaction products that are not produced when the domain is positioned at its naturally-occurring site in the isoprenoid synthase polypeptide.Type: ApplicationFiled: November 23, 2010Publication date: April 7, 2011Inventors: Joseph Chappell, Kyoungwhan Back
-
Publication number: 20110081343Abstract: The present invention includes isolated anti-Langerin vaccines, methods for making and using an isolated anti-Langerin antibody or binding fragment thereof and one or more antigenic peptides at the carboxy-terminus of the isolated anti-Langerin antibody, wherein when two or more antigenic peptides are present, the peptides are separated by the one or more linker peptides that comprise at least one glycosylation site. The present invention also includes isolated vectors for the expression of the anti-Langerin antigen delivery vectors and their manufactures and use.Type: ApplicationFiled: September 14, 2010Publication date: April 7, 2011Applicant: BAYLOR RESEARCH INSTITUTEInventors: Jacques F. Banchereau, Gerard Zurawski, Sandra Zurawski, Eynav Klechevsky, SangKon Oh
-
Publication number: 20110083230Abstract: According to this invention, a gene cluster involved in the non-mevalonate pathway of Hevea brasiliensis was obtained and nucleotide sequences of these genes were determined. The gene cluster according to this invention involved in the IPP biosynthesis in the non-mevalonate pathway is involved in the biosynthesis of vitamin E and carotenoids. Therefore, the Hevea brasiliensis obtained by introducing the gene cluster of the present invention can be expected to produce high-quality rubber with improved permanence.Type: ApplicationFiled: September 2, 2010Publication date: April 7, 2011Applicant: BRIDGESTONE CORPORATIONInventors: Eiichiro FUKUSAKI, Tomoki Sando, Norie Watanabe, Akio Kobayashi, Teuku Tajuddin
-
Patent number: 7919284Abstract: A microorganism belonging to the family Enterobacteriaceae, which has an L-amino acid-producing ability and has been modified so that the kdp system is enhanced, is cultured in a medium to produce and accumulate an L-amino acid in the medium or cells of the microorganism, and the L-amino acid is collected from the medium or cells to produce the L-amino acid.Type: GrantFiled: July 6, 2009Date of Patent: April 5, 2011Assignee: Ajinomoto Co., Inc.Inventors: Rie Takikawa, Yoshihiko Hara
-
Publication number: 20110076730Abstract: Improved enzyme systems, recombinant cells, and processes employing the same to produce biosynthetic D-1,2,4-butanetriol; D-1,2,4-butanetriol prepared thereby and derivatives thereof; D-1,2,4-butanetriol trinitrate prepared therefrom; and enzymes and genes useful in the enzyme systems and recombinant cells.Type: ApplicationFiled: July 19, 2007Publication date: March 31, 2011Applicant: Board of Trustees of Michigan State UniversityInventors: John W. Frost, Wei Niu
-
Patent number: 7914985Abstract: Novel alanine transaminase (ALT) polypeptides and the use thereof as a diagnostic marker to predict and monitor tissue damage and/or tissue malfunction. The ALT polypeptides are murine and/or rattus ALT polypeptides and said ALT polypeptides are used to detect, predict and/or determine hepatic processes of an animal, particularly mice and/or rats.Type: GrantFiled: April 19, 2005Date of Patent: March 29, 2011Assignee: University of Maryland, BaltimoreInventors: Da-Wei Gong, Alan R. Shuldiner, Rong-Ze Yang
-
Publication number: 20110070595Abstract: The present invention relates to immobilization compounds and methods useful for the identification of PARP interacting compounds or for the purification or identification of PARP proteins.Type: ApplicationFiled: May 14, 2009Publication date: March 24, 2011Inventors: Gerard Drewes, Valerie Reader
-
Patent number: 7910345Abstract: Compositions and methods of producing components of protein biosynthetic machinery that include orthogonal tRNAs, orthogonal aminoacyl-tRNA synthetases, and orthogonal pairs of tRNAs/synthetases, which incorporate keto amino acids into proteins are provided. Methods for identifying these orthogonal pairs are also provided along with methods of producing proteins with keto amino acids using these orthogonal pairs.Type: GrantFiled: August 7, 2008Date of Patent: March 22, 2011Assignee: The Scripps Research InstituteInventors: Peter G. Schultz, Lei Wang
-
Patent number: 7910346Abstract: The present invention relates to compositions and methods for the diagnosis and treatment of obesity and related metabolic disorders. The invention provides isolated nucleic acids molecules, designated DGAT2 family member nucleic acid molecules, which encode diacylglycerol acyltransferase family members. The invention also provides recombinant expression vectors containing DGAT2 family member nucleic acid molecules, host cells into which the expression vectors have been introduced, and nonhuman transgenic animals in which a DGAT2 family member gene has been introduced or disrupted. The invention still further provides isolated DGAT2 family member proteins, fusion proteins, antigenic peptides and anti-DGAT2 family member antibodies. Methods of use of the provided DGAT2 family member compositions for screening, diagnostic and therapeutic methods in connection with obesity disorders are also disclosed.Type: GrantFiled: February 17, 2009Date of Patent: March 22, 2011Assignee: Millennium Pharmaceuticals, Inc.Inventors: Ruth E. Gimeno, Rosana Kapeller-Libermann, Zhidan Wu, Brian K. Hubbard
-
Publication number: 20110064663Abstract: The invention relates generally to beta (1,4)-galactosyltransferase I mutants having altered donor and acceptor specificities, and methods of use thereof. In addition, the invention relates to methods for synthesizing oligosaccharides using the beta (1,4)-galactosyltransferase I mutants and to using the beta (1,4)-galactosyltransferase I mutants to conjugate agents, such as therapeutic agents or diagnostic agents, to acceptor molecules.Type: ApplicationFiled: August 22, 2007Publication date: March 17, 2011Applicants: SECRETARY, DEPARTMENT OF HEALTH AND HUMAN SERVICESInventors: Pradman K. Qasba, Boopathy Ramakrishnan, Elizabeth Boeggeman
-
Patent number: 7906707Abstract: Nucleic acid molecules are described which encode polypeptides having the enzymatic activity of a fructosyltransferase. Also, vectors, host cells and transgenic plants are described which contain such nucleic acid molecules. Furthermore, processes for producing polyfructose, particularly that of the inulin type, using the hosts described and/or the fructosyltransferase produced by them are described.Type: GrantFiled: April 12, 2007Date of Patent: March 15, 2011Assignee: Max-Planck-Gesellschaft zur Forderund der Wissenschaften B.V.Inventors: Arnd G. Heyer, Jochen Rehm, Regina Wendenburg
-
Patent number: 7906329Abstract: The present invention relates to the field glycosylation engineering of proteins. More particular, the present invention is directed to the glycosylation engineering of proteins to provide proteins with improved therapeutic properties, e.g., antibodies, antibody fragments, or a fusion protein that includes a region equivalent to the Fc region of an immunoglobulin, with enhanced Fc-mediated cellular cytotoxicity.Type: GrantFiled: May 14, 2003Date of Patent: March 15, 2011Assignee: Glycart Biotechnology AGInventors: Pablo Umana, Joel Jean-Mairet, James E Bailey