Transferase Other Than Ribonuclease (2.) Patents (Class 435/193)
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Publication number: 20110283424Abstract: The present invention relates to genes for enzymes having the activity of transferring a methyl group to lignans, plants with an altered lignan composition using these methyltransferases, and so on. More particularly, the present invention relates to enzyme genes having the activity of synthesizing methylated lignans, preferably enzyme genes having the activity of synthesizing sesame-derived methylated lignans, and use thereof.Type: ApplicationFiled: June 6, 2011Publication date: November 17, 2011Applicant: Suntory Holdings LimitedInventor: Eiichiro ONO
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Patent number: 8058046Abstract: The invention relates to sesquiterpene synthases from Patchouli plants (Pogostemon cablin), and methods of their production and use. In one embodiment, the invention provides nucleic acids comprising a nucleotide sequence as described herein that encodes for at least one sesquiterpene synthase. In a further embodiment, the invention also provides for sesquiterpene synthases and methods of making and using these enzymes. For example, sesquiterpene synthases of the invention may be used to convert farnesyl-pyrophosphate to various sesquiterpenes including patchoulol, ?-curcumene and other germacrane-type sesquiterpenes.Type: GrantFiled: February 5, 2009Date of Patent: November 15, 2011Assignee: Firmenich SAInventors: Michel Schalk, Fabienne Deguerry
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Patent number: 8058053Abstract: The present invention relates to methods and genetically engineered methylotrophic yeast strains for producing glycoproteins with mammalian-like glycosylation. The present invention also relates to vectors useful for generating methylotrophic yeast strains capable of producing glycoproteins with mammalian-like glycosylation. Glycoproteins produced from the genetically engineered methylotrophic yeast strains are also provided.Type: GrantFiled: February 11, 2009Date of Patent: November 15, 2011Assignees: VIB, VZW, Universiteit Gent, Research Corporation Technologies, Inc.Inventors: Roland Contreras, Nico L. M. Callewaert, Wouter Vervecken, Vladimir Kaigorodov
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Patent number: 8058034Abstract: A D-aminotransferase can be modified so as to efficiently produce (2R,4R)-monatin having high sweetness intensity from 4-(indol-3-ylmethyl)-4-hydroxy-2-oxoglutaric acid by substituting an amino acid at least at one of positions (positions 100, 180 to 183, 243 and 244) involved in efficiently producing the (2R,4R)-monatin in an amino acid sequence of a wild-type D-aminotransferase represented in SEQ ID NO:2.Type: GrantFiled: April 12, 2010Date of Patent: November 15, 2011Assignee: Ajinomoto Co., Inc.Inventors: Masakazu Sugiyama, Kunihiko Watanabe, Tatsuki Kashiwagi, Ei-ichiro Suzuki
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Patent number: 8058038Abstract: 4-(Indol-3-ylmethyl)-4-hydroxy-2-oxoglutarate, which is useful as an intermediate in the synthesis of monatin, may be synthesized from indole pyruvic acid and pyruvic acid (and/or oxalacetic acid) by using a novel aldolase derived from the genus Pseudomonas, Erwinia, Flavobacterium, or Xanthomonas.Type: GrantFiled: December 19, 2007Date of Patent: November 15, 2011Assignee: Ajinomoto Co., Inc.Inventors: Masakazu Sugiyama, Kunihiko Watanabe, Shigeru Kawahara
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Patent number: 8058417Abstract: Production and use of a sulfur-containing compound, tropodithietic acid (TDA), from the roseobacter Silicibacter sp. TM1040 is described. Specifically, a biosynthetic and regulatory pathway for TDA biosynthesis in roseobacters is described. The TDA produced from roseobacters, specifically Silicibacter sp. TM1040, is shown to have antibacterial activity, in particular against Vibrio anguillarium, Vibrio cholerae, Vibrio coralliilyticus, Vibrio shiloi, Halomonas spp., Mycobacterium marinum, Mycobacterium tuberculosis, Pseudomonas elongate, Spongiobacter nikelotolerans, and Staphylococcus aureus (MRSA).Type: GrantFiled: May 26, 2009Date of Patent: November 15, 2011Inventors: Robert Belas, Haifeng Geng, Ryan Powell
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Publication number: 20110275135Abstract: Isolated and/or purified polypeptides and nucleic acid sequences encoding polypeptides from Alicyclobacillus acidocaldarius are provided. Further provided are methods for modulating or altering recombination inside or outside of a cell using isolated and/or purified polypeptides and/or nucleic acid sequences from Alicyclobacillus acidocaldarius.Type: ApplicationFiled: May 5, 2010Publication date: November 10, 2011Applicant: Battelle Energy Alliance, LLCInventors: Brady D. Lee, Deborah T. Newby, Jeffrey A. Lacey, David N. Thompson, Vicki S. Thompson, William A. Apel, Francisco F. Roberto, David W. Reed
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Publication number: 20110275526Abstract: A glycosyl transferase from Chinese hamster and related methods are described.Type: ApplicationFiled: November 10, 2010Publication date: November 10, 2011Applicant: MOMENTA PHARMACEUTICALS, INC.Inventor: James W. Meador, III
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Publication number: 20110275119Abstract: The present invention aims to synthesize a polypeptide having an unnatural structure at the N-terminus via a biosynthetic process by translation of amino acid sequence information encoded by a nucleic acid. A polypeptide having any amino acid at the N-terminus is synthesized by using an ARS ribozyme that catalyzes the acylation of tRNA with any amino acid to attach any amino acid to an initiator tRNA, thereby initiating a translation with the initiator tRNA.Type: ApplicationFiled: November 13, 2007Publication date: November 10, 2011Inventors: Hiroaki Suga, Hiroshi Murakami, Yuki Goto, Atsushi Ohta
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Patent number: 8053634Abstract: A method of acylating the 3? position of anthocyanin using an enzyme that transfers an aromatic acyl group to a sugar at the 3? position of anthocyanin or a gene encoding the enzyme.Type: GrantFiled: October 28, 2005Date of Patent: November 8, 2011Assignee: Suntory Holdings LimitedInventors: Yoshikazu Tanaka, Yukihisa Katsumoto, Masako Mizutani, Yuko Fukui, Junichi Togami
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Patent number: 8053637Abstract: Reported herein are methods for obtaining stably transformed callus in Taxus media ‘Hicksii’, including particularly methods that involve using needles, stem, or bark peel as explant material for transformation. Also provided are descriptions of several promoter activities in directing reporter gene expression in Taxus media cells, in particular cells in suspension cultures, callus and needles. Transgenic plants (e.g., Taxus plants), plant cells, cell lines, and tissues (including seeds) are also provided, in particular those that express one or more enzymes in a paclitaxel biosynthesis pathway.Type: GrantFiled: January 3, 2007Date of Patent: November 8, 2011Assignee: Dianaplantsciences, Inc.Inventors: Helena V. Mathews, Vaka S. Reddy, Allan H. Lammers
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Publication number: 20110271401Abstract: A process for the production of transgenic plants that have a high starch content and yield and a high amylose/amylopectin ratio. The alpha-1,4-glucan phosphorylases (GPs) catalyze the reversible cutting of bonds ?-1,4 of the non-reducing ends of homopolysaccharides with at least 5 glucose molecules such as starch, maltodextrin and glycogen, leading to production of glucose-1-phosphate. The GPs in bacteria and animal cells are responsible for the breakdown of glycogen. Although the increase in GP activity leads to a reduction in intracellular levels of glycogen in bacteria and animal cells, this invention discloses the production of plants that have high starch levels and yields and high amylose/amylopectin ratio, as result of the expression of genes coding for GPs.Type: ApplicationFiled: September 10, 2009Publication date: November 3, 2011Applicant: IDEN BIOTECHNOLOGY, S.L.Inventors: Francisco Javier Pozueta Romero, Miren Edurne Baroja Fernández, Francisco José Muñoz Pérez, Nora Alonso Casajús
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Publication number: 20110271404Abstract: The present invention relates generally to the field of molecular biology and concerns a method for enhancing various economically important yield-related traits in plants. More specifically, the present invention concerns a method for enhancing yield-related traits in plants by modulating expression in a plant of a nucleic acid encoding an ASPAT (Asparatate AminoTransferase) polypeptide. The present invention also concerns plants having modulated expression of a nucleic acid encoding an ASPAT polypeptide, which plants have enhanced yield-related traits relative to control plants. The invention also provides hitherto unknown ASPAT-encoding nucleic acids and constructs comprising the same, useful in performing the methods of the invention.Type: ApplicationFiled: September 21, 2009Publication date: November 3, 2011Applicant: BASF Plant Science GmbHInventors: Yves Hatzfeld, Valerie Frankard, Christophe Reuzeau
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Patent number: 8048662Abstract: Isolated sulfotransferase nucleic acid molecules that include a nucleotide sequence variant and nucleotides flanking the sequence variant are described, as well as sulfotransferase allozymes. Methods for determining if a mammal is predisposed to cancer also are described.Type: GrantFiled: April 5, 2011Date of Patent: November 1, 2011Assignee: Mayo Foundation for Medical Education and ResearchInventors: Araba A. Adjei, Eric D. Wieben, Richard M. Weinshilboum, Bianca A. Thomae
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Patent number: 8048647Abstract: The present invention relates to a method for producing optically active IHOG, which can in turn be used for the production of monatin. The present invention further relates to a method for producing optically active monatin, and aldolase used for these methods. As such, the present invention enables the synthesis of 4-(Indole-3-ylmethyl)-4-hydroxy-2-oxoglutaric acid with high optical purity, which is useful as an intermediate in the synthesis of optically active monatin, from indole pyruvic acid and pyruvic acid (or oxaloacetic acid).Type: GrantFiled: December 11, 2009Date of Patent: November 1, 2011Assignee: Ajinomoto Co., Inc.Inventors: Masakazu Sugiyama, Kunihiko Watanabe, Kenichi Mori, Hiroyuki Nozaki
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Patent number: 8048653Abstract: The present invention relates to ?9 elongases, which have the ability to convert linoleic acid [18:2, LA] to eicosadienoic acid [20:2, EDA]. Isolated nucleic acid fragments and recombinant constructs comprising such fragments encoding ?9 elongase along with methods of making long-chain polyunsaturated fatty acids (PUFAs) using these ?9 elongases in plants and oleaginous yeast are disclosed.Type: GrantFiled: November 17, 2009Date of Patent: November 1, 2011Assignee: E.I. du Pont de Nemours and CompanyInventors: Howard Glenn Damude, Brian McGonigle, Zhixiong Xue, Quinn Qun Zhu
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Publication number: 20110262485Abstract: Novel molecules termed STING which include nucleic acids, polynucleotides, oligonucleotides, peptides, mutants, variants and active fragments thereof, modulate innate and adaptive immunity in a subject.Type: ApplicationFiled: August 4, 2009Publication date: October 27, 2011Applicant: University of MiamiInventor: Glen N. Barber
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Publication number: 20110262995Abstract: Compositions and methods for conferring herbicide resistance to bacteria, plants, plant cells, tissues and seeds are provided. Compositions include nucleic acid molecules encoding herbicide resistance or tolerance polypeptides, vectors comprising those nucleic acid molecules, and host cells comprising the vectors. The nucleotide sequences of the invention can be used in DNA constructs or expression cassettes for transformation and expression in organisms, including microorganisms and plants. Compositions also comprise transformed bacteria, plants, plant cells, tissues, and seeds. In particular, the present invention provides for isolated nucleic acid molecules comprising the nucleotide sequence set forth in SEQ ID NO:1 or 14, a nucleotide sequence encoding the amino acid sequence shown in SEQ ID NO:2, the herbicide resistance nucleotide sequence deposited in a bacterial host as Accession Nos. NRRL B-30931, as well as variants and fragments thereof.Type: ApplicationFiled: July 1, 2011Publication date: October 27, 2011Inventors: Cheryl Peters, Brian Vande Berg, Brian Carr, Daniel John Tomso
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Publication number: 20110265197Abstract: The present invention provides methods of detecting cancer using biomarkers.Type: ApplicationFiled: July 16, 2009Publication date: October 27, 2011Applicant: Dana-Farber Cancer Institute, Inc.Inventors: Ronald Depinho, Zhihu Ding, Lynda Chin
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Publication number: 20110263435Abstract: Genetic markers are disclosed with a useful association with boar taint that can be used for screening and selection of pigs for those with more favorable boar taint characteristics associated with androstenone/skatole metabolism. Specific polymorphic alleles of the 3?HSD, 3?HSD, CYP17A1, CYP2A, CYP2E1, CYTB5, BAC-CT and/or SULT1A1 genes are disclosed for tests to screen pigs to determine those more likely to produce desired boar taint traits.Type: ApplicationFiled: January 12, 2007Publication date: October 27, 2011Applicant: UNIVERSITY OF GUELPHInventors: E. James Squires, Dominique Rocha, John Peacock, Zhihong Lin, Nader Deeb
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Patent number: 8044261Abstract: Proteins are provided herein, including proteins capable of catalyzing the acetylation of glyphosate and other structurally related proteins. Also provided are polynucleotides capable of encoding these proteins, compositions that include one or more of these proteins and/or polynucleotides, recombinant cells and transgenic plants comprising these compounds, diversification methods involving the compounds, and methods of using the compounds. Some of the methods and compounds provided herein can be used to render an organism, such as a plant, resistant to glyphosate.Type: GrantFiled: August 3, 2009Date of Patent: October 25, 2011Assignees: E. I. du Pont de Nemours, Verdia Inc., Pioneer Hi-Bred International, Inc.Inventors: Linda A. Castle, Dan Siehl, Lorraine Giver, Jeremy Minshull, Cristina Ivy, Yong Hong Chen, Phillip A. Patten, Nicholas B. Duck, Rebecca Gorton, Billy Fred McCutchen, Roger Kemble
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Patent number: 8043837Abstract: This invention relates to polypeptides having aldolase activity, including pyruvate activity such as, without limitation, HMG and/or KHG aldolase activity, polynucleotides encoding these polypeptides, and methods of making and using these polynucleotides and polypeptides. In some embodiments, the invention is directed to polypeptides having aldolase activity, including pyruvate activity such as, without limitation, HMG and/or KHG aldolase activity, including thermostable and thermotolerant activity, and polynucleotides encoding these enzymes, and making and using these polynucleotides and polypeptides. The polypeptides in accordance with the invention can be used in a variety of pharmaceutical, agricultural and industrial contexts.Type: GrantFiled: March 7, 2007Date of Patent: October 25, 2011Assignee: Cargill, IncorporatedInventors: Ellen Burke, Steven John Gort, Paula M. Hicks, Peter Luginbuhl, Sara C. McFarlan, Toby Richardson, Christopher Solheid, David Weiner, Lishan Zhao
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Publication number: 20110256589Abstract: The present invention provides improved variants of T7 RNA polymerase by introducing novel mutations which lead to improved thermostability of the enzyme. According to the invention, amino acid substitutions at the positions Val426, Ser633, Val650, Thr654, Ala702, Val795, and combinations thereof are advantageous.Type: ApplicationFiled: March 23, 2011Publication date: October 20, 2011Inventors: Harald Sobek, Johann-Peter Thalhofer, Rainer Mueller, Manfred Schmidt, Michael Greif, Armin Ruf, Christian Rudolph
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Publication number: 20110256606Abstract: The invention provides methods and compositions (such as for example, culture media) for culturing Clostridium difficile and producing the C. difficile Toxins A and B.Type: ApplicationFiled: September 24, 2009Publication date: October 20, 2011Applicant: Sanofi Pasteur Biologics Co.Inventors: Aiqi Fang, Andrew James White, Seshu Tummala, Lee Wilson
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Patent number: 8039237Abstract: Genetically engineered organisms for production of PHA copolymers containing 2-hydroxyacid monomers and the methods of making and using thereof have been developed. The copolymers containing 2-hydroxyacid monomers can be synthesized via biosynthesis by the action of a PHA polymerase in a living cell. By changing the genetic background of the cells, one can control specific metabolic pathways allowing control of the level of glycolic acid co-monomer in the PHA polymer.Type: GrantFiled: May 7, 2003Date of Patent: October 18, 2011Assignees: Metabolix, Inc., Tepha, Inc.Inventors: David P. Martin, Frank A. Skraly
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Patent number: 8039794Abstract: Methods are described for measuring the amount of a methylation TPMT enzyme product in a sample. More specifically, mass spectrometric methods are described for detecting and quantifying 6-MMP or isotopically labeled 6-MMP in a test sample utilizing mass spectrometric techniques and for using such methods to determine the activity of TPMT enzyme that is present in a sample.Type: GrantFiled: December 16, 2008Date of Patent: October 18, 2011Assignee: Quest Diagnostics Investments IncorporatedInventors: William F. Haddon, Nigel J. Clarke
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Publication number: 20110252509Abstract: This invention relates generally to a method for producing a transgenic cell with increased gamma-aminobutyric acid (GABA) content as compared to a corresponding non-transformed wild type cell.Type: ApplicationFiled: October 23, 2009Publication date: October 13, 2011Applicant: BASF Plant Sceince GmbHInventors: Janneke Hendriks, Hardy Schön, Oliver Thimm, Volker Haake, Gunnar Plesch, Piotr Puzio, Astrid Blau, Michael Manfred Herold, Birgit Wendel, Beate Kamlage, Florian Schauwecker
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Publication number: 20110251094Abstract: The application discloses new biomarkers for hypertensive disorders of pregnancy and particularly preeclampsia; methods for the diagnosis, prediction, prognosis and/or monitoring said disorders based on measuring said biomarkers; and kits and devices for measuring said biomarker and/or performing said methods.Type: ApplicationFiled: April 13, 2011Publication date: October 13, 2011Applicant: Pronota N.V.Inventor: Koen Kas
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Publication number: 20110244547Abstract: Isolated sulfotransferase nucleic acid molecules that include a nucleotide sequence variant and nucleotides flanking the sequence variant are described, as well as sulfotransferase allozymes. Methods for determining if a mammal is predisposed to cancer also are described.Type: ApplicationFiled: April 5, 2011Publication date: October 6, 2011Applicant: MAYO FOUNDATION FOR MEDICAL EDUCATION AND RESEARCHInventors: Araba A. Adjei, Eric D. Wieben, Richard M. Weinshilboum, Bianca A. Thomae
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Patent number: 8030028Abstract: Compositions and methods of producing components of protein biosynthetic machinery that include orthogonal lysyl-tRNAs, orthogonal lysyl-aminoacyl-tRNA synthetases, and orthogonal pairs of lysyl-tRNAs/synthetases, which incorporate homoglutamines into proteins are provided in response to a four base codon. Methods for identifying these orthogonal pairs are also provided along with methods of producing proteins with homoglutamines using these orthogonal pairs.Type: GrantFiled: November 12, 2009Date of Patent: October 4, 2011Assignee: The Scripps Research InstituteInventors: J. Christopher Anderson, Ning Wu, Stephen Santoro, Peter G. Schultz
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Patent number: 8030043Abstract: The present invention provides a novel ?-galactoside-?2,3-sialyltransferase and a nucleic acid encoding the sialyltransferase. The present invention also provides a microorganism producing the sialyltransferase, as well as a method for producing the sialyltransferase using such a microorganism. The present invention further provides a vector carrying a nucleic acid encoding the sialyltransferase, and a host cell transformed with the vector, as well as a method for producing a recombinant ?-galactoside-?2,3-sialyltransferase. The present invention further provides a method for preparing a sialylsugar chain, which uses the sialyltransferase of the present invention.Type: GrantFiled: March 31, 2006Date of Patent: October 4, 2011Assignee: Japan Tobacco Inc.Inventors: Takeshi Yamamoto, Hiroshi Tsukamoto, Yoshimitsu Takakura
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Patent number: 8030044Abstract: The present invention relates to a method of producing a variant lipid acyltransferase enzyme by selecting a parent enzyme which is a lipid acyltransferase enzyme having the amino acid sequence motif GDSX, modifying one or more amino acids to produce a variant lipid acyltransferase, testing the variant lipid acyltransferase for activity on a galactolipid substrate, a phospholipid substrate and/or a triglyceride substrate, selecting a variant enzyme with an enhanced activity towards galactolipids compared with the parent enzyme, and/or preparing a quantity of the variant enzyme. In some embodiments, the variant lipid acyltransferase enzyme may include the amino acid sequence motif GDSX, wherein X is one or more of the following amino acid residues L, A, V, I, F, Y, X, Q, T N, M or S, and wherein the variant enzyme has one or more amino acid modifications compared with a parent sequence.Type: GrantFiled: February 29, 2008Date of Patent: October 4, 2011Assignee: Danisco A/SInventors: Jørn Borch Søe, Jørn Dalgaard Mikkelson, Arno de Kreij
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Patent number: 8030045Abstract: The present disclosure provides novel beta-alanine/alpha ketoglutarate aminotransferase nucleic acid and protein sequences having increased biological activity. Also provided are cells containing such enzymes, as well as methods of their use, for example to produce malonyl semialdehyde and downstream products thereof, such as 3-hydroxypropionic acid and derivatives thereof.Type: GrantFiled: August 17, 2007Date of Patent: October 4, 2011Assignee: Cargill, IncorporatedInventors: Holly Jean Jessen, Hans H. Liao, Steven John Gort, Olga V. Selifonova
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Publication number: 20110236953Abstract: We have performed a proteomic analysis of embryonic cerebrospinal fluid (e-CSF) in human and rats. Based on this discovery, the invention features methods and compositions for cell culture including components of e-CSF or fragments thereof. Also provided are methods for extraction of e-CSF.Type: ApplicationFiled: August 4, 2008Publication date: September 29, 2011Applicant: Beth Israel Deaconness Medical CenterInventors: Christopher A. Walsh, Mauro Zappaterra, Maria Lehtinen
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Patent number: 8026090Abstract: Disclosed are: (A) a polypeptide consisting of the amino acid sequence of SEQ ID NO:2, or (B) a polypeptide comprising an amino acid sequence of SEQ ID NO:2 including deletion, substitution or addition of one or several amino acid residues and having chondroitin synthase activity; a nucleic acid encoding the polypeptide; a method for producing the polypeptide, comprising at least the steps of: (1) expressing the nucleic acid to produce the polypeptide; and (2) collecting the polypeptide produced in the step (1); and a crystal of the polypeptide. The crystal may be a monoclinic or tetragonal crystal.Type: GrantFiled: March 11, 2011Date of Patent: September 27, 2011Assignee: Seikagaku CorporationInventors: Yoshimitsu Kakuta, Takuo Osawa, Nobuo Sugiura, Koji Kimata
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Patent number: 8021857Abstract: Novel proteins are provided herein, including proteins capable of catalyzing the acetylation of glyphosate and other structurally related proteins. Also provided are novel polynucleotides capable of encoding these proteins, compositions that include one or more of these novel proteins and/or polynucleotides, recombinant cells and transgenic plants comprising these novel compounds, diversification methods involving the novel compounds, and methods of using the compounds. Some of the novel methods and compounds provided herein can be used to render an organism, such as a plant, resistant to glyphosate.Type: GrantFiled: August 3, 2009Date of Patent: September 20, 2011Assignees: Pioneer Hi-Bred International, Inc., E.I. du Pont de Nemours, Verdia Inc.Inventors: Linda A. Castle, Dan Siehl, Lorraine Giver, Jeremy Minshull, Cristina Ivy, Yong Hong Chen, Phillip A. Patten, Nicholas B. Duck, Rebecca Gorton, Billy Fred McCutchen, Roger Kemble
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Patent number: 8022101Abstract: A method of treatment of native, non-denatured tissue to increase resistance to tearing, fissuring, rupturing, and/or delamination, comprising the step of: contacting at least a portion of the tissue with an effective amount of a reagent that increases crosslinks in the tissue.Type: GrantFiled: February 28, 2007Date of Patent: September 20, 2011Assignee: Orthopeutics, L.P.Inventor: Thomas P. Hedman
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Publication number: 20110223647Abstract: Compositions and methods for conferring herbicide resistance to bacteria, plants, plant cells, tissues and seeds are provided. Compositions include nucleic acid molecules encoding herbicide resistance or tolerance polypeptides, vectors comprising those nucleic acid molecules, and host cells comprising the vectors. The nucleotide sequences of the invention can be used in DNA constructs or expression cassettes for transformation and expression in organisms, including microorganisms and plants. Compositions also comprise transformed bacteria, plants, plant cells, tissues, and seeds. In particular, the present invention provides for isolated nucleic acid molecules comprising the nucleotide sequence set forth in SEQ ID NO:1, 3, 4, 6, 7, 9, 10, 12, 13, 15, 17, 18, 20, 21, or 23, a nucleotide sequence encoding the amino acid sequence shown in SEQ ID NO:2, 5, 8, 11, 14, 16, 19, or 22, the herbicide resistance nucleotide sequence deposited in a bacterial host as Accession Nos.Type: ApplicationFiled: April 28, 2011Publication date: September 15, 2011Inventors: Cheryl Peters, Brian Vande Berg, Brian Carr, Daniel John Tomso
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Publication number: 20110223648Abstract: Compositions and methods for conferring herbicide resistance or tolerance to bacteria, plants, plant cells, tissues and seeds are provided. Compositions include polynucleotides encoding herbicide resistance or tolerance polypeptides, vectors comprising those polynucleotides, and host cells comprising the vectors. The nucleotide sequences of the invention can be used in DNA constructs or expression cassettes for transformation and expression in organisms, including microorganisms and plants. Compositions also include transformed bacteria, plants, plant cells, tissues, and seeds. In particular, isolated polynucleotides encoding glyphosate resistance or tolerance polypeptides are provided, particularly polypeptide variants of SEQ ID NO:2 and 4. Additionally, amino acid sequences corresponding to the polynucleotides are encompassed.Type: ApplicationFiled: May 5, 2011Publication date: September 15, 2011Inventors: Volker Heinrichs, Laura Cooper Schouten, Brian Vande Berg
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Publication number: 20110224106Abstract: A method is provided for generating single stranded circular nucleic acid from a sample of target nucleic acid. A complex comprising a transposase and a plurality of hairpin polynucleotides is formed with each of the hairpin polynucleotides having a duplex region comprising a transposase recognition sequence. The complex is mixed with the target nucleic acid, thereby fragmenting the target nucleic acid and ligating the hairpin polynucleotides to the target nucleic acid to form hairpin-linked nucleic acid fragments, each having a nucleobase segment gap between each fragment and its corresponding hairpin polynucleotide. The hairpin-linked fragments are contacted with a ligase, thereby ligating the hairpin-linked fragments together to form single-stranded circular nucleic acid comprising a pair of opposing loops and an intervening duplex region comprising a pair of nucleobase segment gaps.Type: ApplicationFiled: March 9, 2011Publication date: September 15, 2011Applicant: Ibis Biosciences, Inc.Inventors: Mark W. Eshoo, John Picuri
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Publication number: 20110223646Abstract: The present invention provides enhanced methods of producing soluble, active eukaryotic glycosyltransferases in prokaryotic microorganisms that have an oxidizing environment.Type: ApplicationFiled: March 28, 2011Publication date: September 15, 2011Applicant: BIOGENERIX AGInventors: Marc F. Schwartz, Tarik Soliman
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Patent number: 8017835Abstract: The present invention relates to transformed plants with an altered terpene content, preferably over-accumulating a mono- or sesqui-terpene. By transformation of plants with genes encoding terpene synthases (TS), and prenyl transferases (PRT), plants accumulating at least 1000 ng/per g of fresh leaf of a specific terpene were obtained. The present invention provides an advantageous system for production of terpenes in that any desired mono- or sesqui-terpene at the choice of the skilled person can be produced in plants. Preferably, the transformed plants contain at least one recombinant plastid targeted TS and PRT.Type: GrantFiled: April 18, 2006Date of Patent: September 13, 2011Assignee: University of Kentucky Research FoundationInventors: Joe Chappell, Shuiqin Wu, Michel Schalk, Athony Clark
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Patent number: 8017362Abstract: The present invention provides a method for producing L-methionine by culturing a microorganism in a medium to produce and accumulate L-methionine in the medium, and collecting the L-methionine from the medium, where the microorganism is deficient in a repressor of L-methionine biosynthesis system and has L-methionine productivity.Type: GrantFiled: September 8, 2009Date of Patent: September 13, 2011Assignee: Ajinomoto Co., Inc.Inventors: Yoshihiro Usuda, Osamu Kurahashi
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Patent number: 8017593Abstract: The invention provides a method for inhibiting ocular neovascularization in a patient. The method comprises administering to a patient an ocular neovascularization inhibiting amount of a water-soluble polypeptide selected from the group consisting of SEQ ID NO: 12, SEQ ID NO: 7, and an ocular neovascularization inhibiting fragment thereof, which includes at least one of amino acid residue signature sequences HVGH (SEQ ID NO:10) and KMSAS (SEQ ID NO:11). A method for assaying the angiogenesis inhibiting activity of a composition is also provided.Type: GrantFiled: January 13, 2009Date of Patent: September 13, 2011Assignee: The Scripps Research InstituteInventors: Paul Schimmel, Keisuke Wakasugi, Martin Friedlander
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Publication number: 20110217235Abstract: The invention generally features compositions and methods based on the structure-based design of alpha 1-3 N-Acetylgalactosaminyltransferase (alpha 3 GaINAc-T) enzymes from alpha 1-3 galactosyltransferase (a3Gal-T) that can transfer 2?-modified galactose from the corresponding UDP-derivatives due to substitutions that broaden the alpha 3Gal-T donor specificity and make the enzyme a3 GaINAc-T.Type: ApplicationFiled: August 22, 2007Publication date: September 8, 2011Applicant: Health and Human ServicesInventors: Pradman K. Qasba, Boopathy Ramakrishnan, Elizabeth Boeggeman, Marta Pasek
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Publication number: 20110219475Abstract: The present invention relates to nucleic acid fragments encoding amino acid sequences for organic acid biosynthetic enzymes in plants, and the use thereof for the modification of, for example, organic acid biosynthesis and secretion in plants. In particularly preferred embodiments, the invention relates to the combinatorial expression of citrate synthase (CS) and/or malate dehydrogenase (MDH) and/or phosphoenolpyruvate carboxylase (PEPC) in plants to modify, for example, organic acid synthesis and secretion.Type: ApplicationFiled: March 8, 2011Publication date: September 8, 2011Applicant: AGRICULTURE VICTORIA SERVICES PTY LTDInventors: German Spangenberg, Michael Emmerling, Eng Kok Ong, Ramiro Mendez, Stephen Panter, Marcel Labandera
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Publication number: 20110219476Abstract: The present invention provides a novel glucuronosyltransferase, a polynucleotide encoding the same (e.g., a polynucleotide comprising a polynucleotide consisting of the nucleotide sequence at positions 1 to 1362 in the nucleotide sequence represented by SEQ ID NO: 7, or a polynucleotide comprising a polynucleotide encoding a protein having the amino acid sequence represented by SEQ ID NO: 8); and so on. A novel glucuronosyltransferase having a broad substrate specificity and others can thus be provided.Type: ApplicationFiled: September 4, 2008Publication date: September 8, 2011Inventors: Eiichiro Ono, Yuko Fukui
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Patent number: 8013114Abstract: The present invention is directed to fusion proteins that can be used to assay gene transfer and expression both in vitro and in vivo. The fusion proteins contain a reporter protein, e.g. a somatostatin receptor, fused to a second protein, which may be a protein fusion tag. Alternatively, a fusion protein may be fused to a leader sequence. A leader sequence may localize an expressed protein, e.g localize a fusion protein to the cell membrane. The invention includes nucleic acids encoding the fusion proteins and methods of assaying for gene expression.Type: GrantFiled: December 13, 2006Date of Patent: September 6, 2011Assignee: The Board of Regents of the University of Texas SystemInventor: Vikas Kundra
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Patent number: 8008547Abstract: Proteins are provided herein, including proteins capable of catalyzing the acetylation of glyphosate and other structurally related proteins. Also provided are polynucleotides capable of encoding these proteins, compositions that include one or more of these proteins and/or polynucleotides, recombinant cells and transgenic plants comprising these compounds, diversification methods involving the compounds, and methods of using the compounds. Some of the methods and compounds provided herein can be used to render an organism, such as a plant, resistant to glyphosate.Type: GrantFiled: April 1, 2009Date of Patent: August 30, 2011Assignees: E.I. duPont de Nemours, Verdia Inc., Pioneer Hi-Bred International Inc,Inventors: Linda A. Castle, Dan Siehl, Lorraine Giver, Jeremy Minshull, Cristina Ivy, Yong Hong Chen, Phillip A. Patten, Rebecca Gorton, Nicholas B. Duck, Billy Fred McCutchen, Roger Kemble
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Publication number: 20110207157Abstract: The invention relates to new proteins called alkylcytosine transferases (ACTs) derived from O6-alkylguanine-DNA alkyltransferase, and to substrates for ACTs specifically transferring a label to these ACTs and to fusion proteins comprising these. The substrates according of the invention are substituted cytosines of formula (I) wherein R1 is an aromatic or a heteroaromatic group, or an optionally substituted unsaturated alkyl, cycloalkyl or heterocyclyl group with the double bond connected to OCH2—; R2 is a linker; and L is a label or a plurality of same or different labels. The invention further relates to methods of transferring label L from these substrates of formula (I) to ACTs and ACT fusion proteins. The system of ACT-compound of formula (I) is particularly suitable for double labelling studies together with the known system O6-alkylguanine-DNA alkyltransferase (AGT)-benzylguanines.Type: ApplicationFiled: July 24, 2007Publication date: August 25, 2011Applicant: Ecole Polytechnique Federale de LausanneInventors: Arnaud Gautier, Kai Johnsson, Maik Kindermann, Alexandrc Juillerat, Florent Beaufils