Triglyceride Splitting (e.g., Lipase, Etc. (3.1.1.3)) Patents (Class 435/198)
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Patent number: 8273555Abstract: Provided is a process for esterifying or transesterifying side chains in polymers, which involves esterifying or transesterifying the carboxyl group having the R4 radical of a copolymer obtained by polymerizing a (meth)acrylate of formula (I), and a monomer of formula (II): wherein R1 and R3 are independently H or CH3, R2 and R4 are independently H or an alkyl radical which is optionally substituted by functional groups such as acryloyl, ether, amino, epoxy, halogen or sulfonic acid groups, and n is from 1 to 40, with an alcohol in the presence of an enzyme which catalyzes the esterifying or transesterifying. Further provided is the esterified or transesterified polymer product and its use.Type: GrantFiled: November 24, 2006Date of Patent: September 25, 2012Assignee: BASF AktiengesellschaftInventors: Dietmar Haering, Lidcay Herrera Taboada, Bernhard Hauer, Thomas Friedrich, Alexander Goethlich
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Patent number: 8263370Abstract: A process for preparing urethane-containing (meth)acrylic esters (U) by reacting a urethane-containing alcohol (A) with a (meth)acrylic ester of a saturated alcohol (G) in the presence of at least one polymerization inhibitor (P) with an enzyme (E) as a catalyst in a reactor, wherein the (meth)acrylic ester of a saturated alcohol (G) and the urethane-containing alcohol (A) are passed continuously through at least one fixed bed reactor filled with an immobilized enzyme (E) as a catalyst.Type: GrantFiled: May 12, 2010Date of Patent: September 11, 2012Assignee: BASF SEInventors: Juergen Daeuwel, Steffen Maurer, Rainer Stuermer
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Publication number: 20120227120Abstract: Provided are hydrolases, including lipases, saturases, palmitases and/or stearatases, and polynucleotides encoding them, and methods of making and using these polynucleotides and polypeptides. Further provided are polypeptides, e.g., enzymes, having a hydrolase activity, e.g., lipases, saturases, palmitases and/or stearatases and methods for preparing low saturate or low trans fat oils, such as low saturate or low trans fat animal or vegetable oils, e.g., soy or canola oils.Type: ApplicationFiled: May 14, 2012Publication date: September 6, 2012Applicant: DSM IP ASSETS B.V.Inventors: Tim HITCHMAN, Christopher L. G. DAYTON, Katie A. KLINE, Jonathan LYON, Mark A. WALL, Nelson R. BARTON
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Patent number: 8257698Abstract: A number of soluble engineered forms of MGLL that are suitable for high-throughput screening and protein crystallization, as well as a crystallized form of monoacylglycerol lipase protein (MGLL) and descriptions of the X-ray diffraction patterns are disclosed. The engineered constructs of MGLL permit the expression and purification of protein suitable for crystallography or high-throughput screening and identification of ligands, which can function as active agents to MGLL. The X-ray diffraction patterns allow the three dimensional structure of MGLL to be determined at atomic resolution so that ligand binding sites on MGLL can be identified and the interactions of ligands with MGLL amino acid residues can be modeled. Models prepared using such maps permit the design of ligands which can function as active agents which include, but are not limited to, those that function as inhibitors of MGLL.Type: GrantFiled: April 24, 2009Date of Patent: September 4, 2012Assignee: Janssen Pharmaceutica N.V.Inventors: Bruce L. Grasberger, Celine Schalk-Hihi, Diane M. Maguire, Frank A. Lewandowski, Cynthia M. Milligan, Richard S. Alexander
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Publication number: 20120219666Abstract: The invention relates to a DNA sequence that encodes a polypeptide with lysophospholipase activity and was isolated from Aspergillus and sequences derived therefrom, polypeptides with lysophospholipase activity encoded by these sequences as well as the use of these polypeptides for improving the filterability of syrups consisting of wheat starch and for related applications.Type: ApplicationFiled: May 10, 2012Publication date: August 30, 2012Applicant: AB ENZYMES GMBHInventors: Khanh Q. Nguyen, Volker Marschner, Kornelia Titze, Bruno Winter
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Patent number: 8241849Abstract: The invention is directed to isolated genomic polynucleotide fragments that encode human lipoprotein-associated phospholipase A2, vectors and hosts containing the fragment and fragments hybridizing to noncoding regions as well as antisense oligonucleotides to these fragments. The invention is further directed to methods of using these fragments to obtain human lipoprotein-associated phospholipase A2 and to diagnose, treat, prevent and/or ameliorate a pathological disorder.Type: GrantFiled: November 25, 2008Date of Patent: August 14, 2012Assignee: Ryogen LLCInventor: James W Ryan
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Patent number: 8241876Abstract: A method is disclosed for the generation of triacylglycerols from gums that have been separated from an oil product. The gums are treated with an enzyme having PLC activity, which results in the formation of diacylglycerols and phosphates, and treated with an enzyme having PLA activity, which results in the formation of lyso-phospholipids and free fatty acids. The diacylglycerols and the free fatty acids from these two separate reactions then combine in the presence of the enzymes to generate new triacylglycerol molecules.Type: GrantFiled: January 7, 2008Date of Patent: August 14, 2012Assignee: Bunge Oils, Inc.Inventor: Christopher L. G. Dayton
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Patent number: 8236533Abstract: The present invention relates to a process for preparing a compound comprising an ?,? amide linkage between a cysteine moiety and a glutamic acid moiety, such as ?-glutamylcysteine or a ?-glutamylcysteine derivative, the process comprising providing a cysteine derivative, a ?-glutamyl donor and an enzyme capable of transferring the ?-glutamyl group to said cysteine derivative in a reaction environment promoting transfer of the ?-glutamyl group to said cysteine derivative. The invention also relates to compounds comprising an ?,? amide linkage between a cysteine moiety and a glutamic acid moiety, such as ?-glutamylcysteine or a ?-glutamylcysteine derivative, when obtained by processes of the invention, and uses thereof.Type: GrantFiled: March 31, 2006Date of Patent: August 7, 2012Assignee: NewSouth Innovations PTY LimitedInventors: Wallace John Bridge, Martin Hani Zarka
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Publication number: 20120196002Abstract: The present invention provides a transformed or transfected Trichoderma reesei cell comprising one or more of: (i) at least one heterologous nucleotide sequence encoding a lipolytic enzyme comprising an amino acid sequence shown as SEQ ID NO: 1 or SEQ ID NO:2, (ii) at least one heterologous nucleotide sequence encoding a lipolytic enzyme wherein the nucleotide sequence comprises the nucleotide sequence shown as SEQ ID NO: 3 or SEQ ID NO: 4, or (iii) at least one heterologous nucleotide sequence encoding a lipolytic enzyme wherein the nucleotide sequence comprises the nucleotide sequence which hybridizes to SEQ ID NO: 3 or SEQ ID NO: 4 or a nucleotide sequence which is at least 40% sequence identity to SEQ ID NO: 3 or SEQ ID NO: 4; and culturing the cell under conditions to allow for expression of said heterologous nucleotide sequence(s) encoding said lipolytic enzyme.Type: ApplicationFiled: April 23, 2010Publication date: August 2, 2012Applicant: DANISCO A/SInventors: Susan Madrid, Cherry Lin, Masoud Rajabi Zargahi, Rikke Høegh Lorentsen, Mai Faurschou Isaksen, Michael Ward
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Publication number: 20120192316Abstract: The present invention relates to isolated polypeptides having lipase activity and isolated polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods for producing and using the polypeptides.Type: ApplicationFiled: April 5, 2012Publication date: July 26, 2012Applicants: Novozymes A/S, Novozymes, Inc.Inventors: Debbie Yaver, Suzanne Otani, Janine Lin, Christopher Amolo, Kim Borch, Shamkant Anant Patkar, Michael Lamsa, Barbara Cherry
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Publication number: 20120183993Abstract: The invention relates to a process for the preparation of a fermentation product from ligno-cellulosic material, comprising the following steps: a) optionally pre-treatment b) optionally washing; c) enzymatic hydrolysis; d) fermentation; and e) optionally recovery of a fermentation product; wherein in step c) an enzyme composition is used that has a temperature optimum of 55 degrees C. or more, the hydrolysis time is 40 hours or more and the temperature is 50 degrees C. or more.Type: ApplicationFiled: October 5, 2010Publication date: July 19, 2012Applicant: DSM IP ASSETS B.V.Inventors: Johannes Petrus Smits, Elisabeth Maria Gierveld, Fop Cornelis Van Der Hor
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Publication number: 20120183995Abstract: The present invention provides cells that have been genetically manipulated to have an altered capacity to produce expressed proteins. In particular, the present invention relates to Gram-positive microorganisms, such as Bacillus species having enhanced expression of a protein of interest, wherein one or more chromosomal genes have been inactivated, and preferably wherein one or more chromosomal genes have been deleted from the Bacillus chromosome. In some further embodiments, one or more indigenous chromosomal regions have been deleted from a corresponding wild-type Bacillus host chromosome.Type: ApplicationFiled: January 23, 2012Publication date: July 19, 2012Applicant: Danisco US Inc.Inventors: Eugenio Ferrari, Carole Harbison, M. Harunur Rashid, Walter Weyler
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Patent number: 8216586Abstract: The invention relates to a newly identified polynucleotide sequence comprising a gene that encodes a novel lipolytic enzyme from Aspergillus niger The invention features the full length nucleotide sequence of the novel gene, the cDNA sequence comprising the full length coding sequence of the novel lipolytic enzyme as well as the amino acid sequence of the full-length functional protein and functional equivalents thereof. The invention also relates to methods of using these enzymes in industrial processes and methods of diagnosing fungal infections. Also included in the invention are cells transformed with a polynucleotide according to the invention and cells wherein a lipolytic enzyme according to the invention is genetically modified to enhance its activity and/or level of expression.Type: GrantFiled: May 6, 2009Date of Patent: July 10, 2012Assignee: DSM IP Assets B.V.Inventors: Richard Albang, Andreas Fritz, Oliver Heinrich, Hilmar Ilgenfritz, Dieter Maier, Christian Wagner, Ulrike Folkers, Beatrix Gerhard, Fabio Spreafico, Lex De Boer, Roelf Bernhard Meima
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Publication number: 20120171189Abstract: The invention relates to functional polypeptides secreted from Botryospaeria rhodina CBS 274.96.Type: ApplicationFiled: February 8, 2012Publication date: July 5, 2012Applicant: NOVOZYMES A/SInventors: Kirk Matthew Schnorr, Lene Lange, Pernille Uldall Bolvig
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Publication number: 20120171738Abstract: The present invention relates to a biocatalytic method of preparing a mono-acylated polyol catalyzed by triacyl-glycerol lipase mutants, as for example derived from Candida antarctica lipase B (CALB); a biocatalytic method of enantioselectively preparing an asymmetric mono-acylated polyol, catalyzed by the same enzyme mutants; as well as the use of a mutated triacylglycerol lipase in a method of preparing mono-acylated polyols. The invention also provides novel mutants, coding sequences thereof, and recombinant microorganisms carrying said coding sequences.Type: ApplicationFiled: September 16, 2010Publication date: July 5, 2012Applicant: BASF SEInventors: Bernhard Hauer, Cecilia Kvarnström Branneby, Karl Hult, Anders Mangnusson, Anders Hamberg
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Publication number: 20120171736Abstract: The present invention discloses a powdery lipase preparation in which the particles constituting the powdery lipase have 3,000 to 40,000 pores/mm2 on the surface thereof, and each pore has a diameter of 0.5 ?m to 6 ?m. This powdery lipase preparation was able to improve lipase activity without using a soybean powder.Type: ApplicationFiled: November 26, 2010Publication date: July 5, 2012Applicant: The Nisshin OilliO Group, Ltd.Inventors: Satoshi Negishi, Yoshie Yamauchi, Yosuke Nakamura, Yuko Toyama
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Patent number: 8202715Abstract: The invention relates to a DNA sequence that encodes a polypeptide with lysophospholipase activity and was isolated from Aspergillus and sequences derived therefrom, polypeptides with lysophospholipase activity encoded by these sequences as well as the use of these polypeptides for improving the filterability of syrups consisting of wheat starch and for related applications.Type: GrantFiled: September 21, 2007Date of Patent: June 19, 2012Assignee: AB Enzymes GmbHInventors: Khanh Q. Nguyen, Volker Marschner, Kornelia Titze, Bruno Winter
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Patent number: 8198054Abstract: This disclosure relates to the synthesis of the compound of formula (I) according to Scheme A below: in which R1, R2 and R3, which may be identical or different, represent, individually and independently, an alkyl group, characterized by an enzymatic hydrolysis reaction that involves placing the compound of formula (II) in contact with an enzyme that performs a chemoselective hydrolysis of only one of the two ester functions of the compound of formula (II) to obtain the compound of formula (I).Type: GrantFiled: January 22, 2008Date of Patent: June 12, 2012Assignees: Zach System, Novacta Biosystem LimitedInventors: Alain Burgos, Jean-Claude Caille, Michelle Lorraine-Gradley
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Patent number: 8198062Abstract: Provided are hydrolases, including lipases, saturases, palmitases and/or stearatases, and polynucleotides encoding them, and methods of making and using these polynucleotides and polypeptides. Further provided are polypeptides, e.g., enzymes, having a hydrolase activity, e.g., lipases, saturases, palmitases and/or stearatases and methods for preparing low saturate or low trans fat oils, such as low saturate or low trans fat animal or vegetable oils, e.g., soy or canola oils.Type: GrantFiled: August 29, 2008Date of Patent: June 12, 2012Assignee: DSM IP Assets B.V.Inventors: Timothy Hitchman, Christopher L. G. Dayton, Katie A. Kline, Jonathan Lyon, Mark Wall, Nelson R. Barton
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Publication number: 20120142073Abstract: Provided in part herein are compositions that include isolated microbial enzymes, such as proteases, lipases, useful in dehairing leather. Also provided are methods for isolating proteases, lipases, from microbes, as well as methods of reusing these enzymes effectively for several cycles in an environment friendly process.Type: ApplicationFiled: November 16, 2010Publication date: June 7, 2012Inventor: Shaon Ray Chaudhuri
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Patent number: 8187854Abstract: The invention provides variant lipases, preferably, variants with reduced tendency to odor generation obtained by introducing mutations in one or more regions identified in a parent lipase.Type: GrantFiled: January 22, 2007Date of Patent: May 29, 2012Assignee: Novozymes A/SInventors: Jesper Vind, Jürgen Carsten Franz Knötzel, Kim Borch, Allan Svendsen, Thomas Honger Callisen, Debbie Yaver, Mads Eskelund Bjornvad, Peter Kamp Hansen, Haiyan Ge, Michael Lamsa
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Patent number: 8183019Abstract: A process is disclosed for preparing (3R,4R)-3-hydroxy-4-hydroxymethylpyrrolidine, the compound of formula (I), or (3S,4S)-3-hydroxy-4-hydroxymethylpyrrolidine, the compound of formula (Ia) involving, as a key step, the enzyme-catalysed enantioselective hydrolysis of a racemic 3,4-trans-disubstituted pyrrolidinone compound of formula (II).Type: GrantFiled: June 3, 2005Date of Patent: May 22, 2012Assignee: Industrial Research LimitedInventors: Dirk Henning Lenz, Jennifer Mary Mason, Keith Clinch, Gary Brian Evans, Peter Charles Tyler
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Patent number: 8183018Abstract: A process for preparing (meth)acrylic esters (F) of alcohols (A) having at least one epoxy group, in which at least one alcohol (A) having at least one epoxy group is esterified with (meth)acrylic acid (S) or is transesterified with at least one (meth)acrylic ester (D) in the presence of at least one enzyme, the alcoholic leaving group being stable under the reaction conditions in the case of the transesterification.Type: GrantFiled: October 23, 2007Date of Patent: May 22, 2012Assignee: BASF AktiengesellschaftInventors: Dietmar Haering, Uwe Meisenburg, Mathieu Chabanas, Gunter Lipowsky
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Publication number: 20120122183Abstract: A method of flocculating a bacterial cell, producing a protein of interest, from a fermentation broth, comprising a) diluting the fermentation broth up to 1000% (w/w) with water; b) adding a divalent salt to a concentration in the fermentation broth of more than 10 milli moles per liter diluted fermentation broth; c) adjusting the phosphate concentration to a concentration in the fermentation broth of more than 10 milli moles per liter diluted fermentation broth; d) adjusting the pH of the diluted fermentation broth to a pH within the range of 6.1-10.5; and e) removing the bacterial cells, whereby a protein solution with a turbidity of less than 100 NTU is obtained.Type: ApplicationFiled: June 30, 2010Publication date: May 17, 2012Applicant: NOVOZYMES A/SInventors: Jakob Rauhe Pedersen, Peter Frode Pind
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Publication number: 20120115186Abstract: The present invention relates to a process for the production of one or more fermentation product from a sugar composition, comprising the following steps: a) fermentation of the sugar composition in the presence of a yeast belonging to the genera Saccharomyces, Kluyveromyces, Candida, Pichia, Schizosaccharomyces, Hansenula, Kloeckera, Schwanniomyces or Yarrowia, and b) recovery of the fermentation product, wherein the yeast comprises the genes araA, araB and araD and the sugar composition comprises glucose, galactose and arabinose.Type: ApplicationFiled: July 6, 2010Publication date: May 10, 2012Applicant: DSM IP ASSETS B.V.Inventors: Paul Klaassen, Gijsberdina Pieternella Van Suylekom, Bianca Elisabeth Maria Gielesen, Nicolette Jasmijn Broers, Beate Wiedemann, Wilhelmus Theodorus Antonius Maria De Laat
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Publication number: 20120107905Abstract: The present invention relates to fungal serine protease variants, which comprise an amino acid substitution of valine at position 208 of the parent Fusarium equiseti Fe_RF6318 serine protease, wherein the position of the substitution corresponds to the amino acid sequence of the mature Fe_RF6318 enzyme defined in SEQ ID NO:2. The variants have improved thermal stability and/or detergent stability compared to the parent Fe_RF6318 enzyme. Preferably the substitution is V208I and more preferably the variants comprise additional amino acid changes which further increase the stability. Also disclosed are nucleic acid sequences encoding said protease variants as well as recombinant vectors and host cells for the production of the variants.Type: ApplicationFiled: October 28, 2011Publication date: May 3, 2012Applicant: AB ENZYMES OYInventors: Kari Juntunen, Leena Valtakari, Nina Hakulinen, Marja Paloheimo
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Publication number: 20120100581Abstract: The invention provides novel polypeptides having phospholipase activity, including, e.g., phospholipase A, B, C and D activity, patatin activity, phosphatidic acid phosphatases (PAP)) and/or lipid acyl hydrolase (LAH) activity, nucleic acids encoding them and antibodies that bind to them. Industrial methods, e.g., oil degumming, and products comprising use of these phospholipases are also provided.Type: ApplicationFiled: April 20, 2011Publication date: April 26, 2012Applicant: VERENIUM CORPORATIONInventors: SVETLANA GRAMATIKOVA, GEOFF HAZLEWOOD, DAVID LAM, NELSON R. BARTON, BLAKE G. STURGIS, DAN E. ROBERTSON, JINCAI LI, JOEL A. KREPS, RODERICK JAMES FIELDING, ROBERT C. BROWN, AMIT VASAVADA, XUQIU TAN, ADRIAN BADILLO, WILHELMUS P. VAN HOEK, GISELLE JANSSEN, CHARLES ISAAC, MARK J. BURK
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Publication number: 20120097194Abstract: Disclosed herein are materials including a polymeric materials such as a coating, a plastic, a laminate, a composite, an elastomer, an adhesive, or a sealant; a surface treatment such as a coating, a textile finish or a wax; a filler for such a polymeric material or a surface treatment, which includes an enzyme such as an esterase (e.g., a lipolytic enzyme, an organophosphorus compound degradation enzyme), wherein the enzyme decontaminates a chemical from the surface of the material. Also disclosed herein are methods of cleaning a surface of a material that comprises an enzyme.Type: ApplicationFiled: January 11, 2011Publication date: April 26, 2012Applicant: REACTIVE SURFACES, LTD.Inventors: C. Steven McDaniel, Eric B. Williams, James W. Rawlins, Melinda Wales
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Publication number: 20120093850Abstract: Staphylococcus aureus Hla polypeptides having various deletions, insertions, and/or mutations which are useful for immunisation are provided herein. Also provided herein are Hla heptamers which are non-haemolytic. Additionally, an effective Staphylococcus aureus vaccine may require several antigenic components, and so various combinations of S. aureus antigens, including Hla polypeptides having deletions, insertions, and/or mutations, are identified for use in immunisation. These polypeptides may optionally be used in combination with S. aureus saccharides.Type: ApplicationFiled: September 15, 2011Publication date: April 19, 2012Applicant: NOVARTIS AGInventors: Fabio BAGNOLI, Massimiliano Biagini, Luigi Fiaschi, Guido Grandi, Ravi Pratap Narayan Mishra, Nathalie Norais, Maria Scarselli
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Publication number: 20120094358Abstract: Biomass (e.g., plant biomass, animal biomass, microbial, and municipal waste biomass) is processed to produce useful products, such as food products and amino acids.Type: ApplicationFiled: December 6, 2011Publication date: April 19, 2012Applicant: XYLECO, INC.Inventor: Marshall Medoff
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Patent number: 8148096Abstract: A method for the detection and/or measurement in vitro of a lipase or phospholipase activity, including the addition of a sample likely to contain said lipase or phospholipase into the wells of microtitration plates coated with a layer of a lipid substrate which is able to be hydrolyzed by the lipase or phospholipase by releasing ?-eleostearic acid, and the detection and/or the measurement of the lipase or phospholipase activity by UV spectrophotometry of the ?-eleostearic acid released during the previous stage. The application of this method to the in vitro diagnosis of pathologies linked to an increase in the plasma lipase level.Type: GrantFiled: February 10, 2006Date of Patent: April 3, 2012Assignee: Centre National de la Recherche ScientifiqueInventors: Robert Verger, Carole Serveau-Avesque, Henri Chahinian
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Patent number: 8148097Abstract: An in vitro screening method for identifying a compound with iPLA2? modulating activity.Type: GrantFiled: November 12, 2008Date of Patent: April 3, 2012Assignee: Washington UniversityInventors: Richard W. Gross, Christopher Jenkins
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Patent number: 8148118Abstract: The present invention relates to a recyclable method to prepare chirally pure epoxides directly from olefins using a novel chiral acid 2,3:4,6-di-O-isopropylidene-2-keto-L-gulonic acid monohydrate as chiral inducer and anhydrous hydrogen peroxide in the form of urea hydrogen peroxide adduct (UHP) as oxidant and lipase enzyme as catalyst. Under the influence of lipase, the chiral acid 2,3:4,6-di-O-isopropylidene-2-keto-L-gulonic acid monohydrate is converted to per-acid in situ by the oxidant urea hydrogen peroxide which in turn transfers oxygen to olefin inducing chirality to the product epoxide. Using this technique, important epoxide building blocks of different industrially important compounds can be prepared with good yield and moderate enantioselectivity. Chiral epoxidation of olefins with environment friendly catalytic system that can be recycled is highly desirable in the context of Green Chemistry for preparation of many industrially important compounds.Type: GrantFiled: November 27, 2009Date of Patent: April 3, 2012Assignee: Council of Scientific & Industrial ResearchInventors: Amrit Goswami, Kuladip Sarma, Ajit Kumar Hazarika
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Publication number: 20120079626Abstract: We describe a plant lipase polypeptide and nucleic acids that encode said polypeptide which has homology to a patatin and which has phospholipase and/or triacylglycerol lipase activity.Type: ApplicationFiled: December 2, 2011Publication date: March 29, 2012Applicant: UNIVERSITY OF YORKInventor: Peter Eastmond
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Publication number: 20120058930Abstract: The present invention relates to isolated polypeptides having lipase activity and isolated polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods for producing and using the polypeptides.Type: ApplicationFiled: November 10, 2011Publication date: March 8, 2012Applicants: NOVOZYMES A/S, NOVOZYMES, INC.Inventors: Debbie Yaver, Suzanne Otani, Janine Lin, Christopher Amolo, Kim Borch, Shamkant Anant Patkar, Michael Lamsa, Barbara Cherry
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Publication number: 20120058514Abstract: The objective of the present invention is to obtain a low temperature producing enzyme and more particularly, the enzyme provides a bifunctional purpose of varying its enzyme activity into activity of another enzyme. The present invention has overcome problems such as ability of obtaining an enzyme at low temperature.Type: ApplicationFiled: September 8, 2010Publication date: March 8, 2012Applicant: UNIVERSITY PUTRA MALAYSIAInventors: RAJA NOOR ZALIHA, ABU BAKAR SALLEH, MAHIRAN BASRI, MOHD SHUKURI BIN MOHAMAD ALI
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Publication number: 20120053080Abstract: Methods for detecting cancer as well as methods of diagnosis of cancer by detecting proteins secreted into biological fluids are disclosed The invention was first applied to detecting proteins secreted into serum and urine However, it is understood that the methods have broader application to developing tools and systems for detecting proteins secreted into other biological fluids such as, but not limited to, saliva, spinal fluid, seminal fluid, vaginal fluid, and ocular fluid Reliable detection of proteins secreted into biological fluids provided by embodiments of the methods will enable more timely and accurate detection and diagnosis of cancer.Type: ApplicationFiled: February 19, 2010Publication date: March 1, 2012Inventors: Juan Cui, J. David Puett, Seulgi Hong, Ying Xu
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Patent number: 8110386Abstract: A lipase powder which is a granulated substance containing a lipase and a solid content of animal milk, a lipase composition wherein said lipase powder is immersed or impregnated in fatty oil, and a method for producing the lipase powder which comprises the step of adding animal milk or cream derived from the animal milk to an aqueous solution containing a lipase, and the step of spray-drying, freeze-drying or solvent-precipitating the mixture thereof are provided. According to the present invention, a lipase powder of which lipase activity and stability are improved can be provided.Type: GrantFiled: December 11, 2008Date of Patent: February 7, 2012Assignee: The Nisshin Oillio Group, Ltd.Inventors: Junko Suzuki, Satoshi Negishi, Yuri Arai, Chika Sakurai
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Publication number: 20120009324Abstract: Disclosed herein is an isolated nucleic acid sequence encoding a recombinant polypeptide that has pancreatic lipase activity. Also disclosed herein are a recombinant vector and a recombinant host cell for producing the aforesaid recombinant polypeptide. The aforesaid recombinant polypeptide is adapted for preparation of an animal feed that is able to facilitate utilization of fats therein regarding pigs (especially postweaning pigs) and to enhance growth performance of the pigs.Type: ApplicationFiled: February 16, 2011Publication date: January 12, 2012Applicant: Livestock Research Institute Council of AgricultureInventor: Fang-Chueh Liu
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Publication number: 20120009613Abstract: Use of an inhibitor or activator of the triglyceride hydrolyse activity of a protein comprising a polypeptide strand encoded by the DNA sequence according to SEQ No. 1 for the preparation of a pharmaceutical composition for the treatment of medical disorders where it is desirable to modulate the activity of a protein encoded by the DNA sequence according to SEQ No. 1.Type: ApplicationFiled: June 3, 2011Publication date: January 12, 2012Applicant: Karl-Franzens-Universitat GrazInventors: Rudolpf ZECHNER, Robert Zimmermann, Juliane G. Strauss, Gunter Hammerle, Achim Lass
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Publication number: 20120003715Abstract: A method for expressing and industrial scale production of recombinant lysosomal enzymes utilizing insect larva including the steps of infecting an insect larva population of the species Spodoptera littoralis via a recombinant baculovirus liquid suspension which permits the expression of at least one gene coding for a protein of interest, at least one of these genes coding for a lysosomal enzyme expressed in the insect larva, collecting of the previously infected insect larva expressing in a sufficient significant quantity the protein of interest and recovering the protein of interest from the collected insect larva.Type: ApplicationFiled: March 13, 2009Publication date: January 5, 2012Applicant: Bioorganic Research and Services S.L.Inventors: Victor Infante Viñolo, Rosa Maria Osuna Aguilar, Juan Jose Infante Viñolo
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Publication number: 20120003716Abstract: Method for producing Yarrowia lipolytica acid-resistant recombinant lipase utilizing a culture medium without any products of animal origin or non-characterized mixtures such as tryptone, peptone or lactoserum, in addition to its uses. Recombinant strain of Yarrow lipolytica producing an excessive amount of lipase Lip2 referred to as YL-LIP2-6C and filed with the Collection Nationale de Cultures de Microorganismes (C.N.C.M.) under number I 3542, on Dec. 15, 2005 and its uses.Type: ApplicationFiled: June 15, 2006Publication date: January 5, 2012Applicant: LABORATOIRES MAYOLY SPINDLERInventors: Yves Leblond, Nicolas Mouz, Alain Marty, Jean-Louis Uribelarrea
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Patent number: 8084025Abstract: A therapeutic agent for the treatment of the symptoms of addiction and the method for preparing the therapeutic agent is disclosed. The therapeutic agent is a stable pharmaceutical preparation containing, but not limited to, digestive/pancreatic enzymes. The therapeutic agent may be manufactured by a variety of encapsulation technologies. Delivery of the therapeutic agent may be made orally, through injection, by adherence of a medicated patch or other method. Further, a method of using of a biomarker, the presence of chymotrypsin in the gastrointestinal tract to determine the presence of symptoms of addiction, and the likelihood of relapsing into addiction is disclosed.Type: GrantFiled: April 20, 2009Date of Patent: December 27, 2011Assignee: Curemark LLCInventor: Joan M. Fallon
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Publication number: 20110312028Abstract: The present invention provides a novel nucleic acid sequence, designated ELIP, encoding a lipolytic enzyme and the corresponding encoded amino acid sequences. The invention also provides expression vectors and host cells comprising a nucleic acid sequence encoding at least one novel lipolytic enzyme, recombinant lipolytic enzyme proteins and methods for producing the same.Type: ApplicationFiled: May 24, 2010Publication date: December 22, 2011Inventors: BRIAN E. JONES, WILLIAM D. GRANT, SHAUN HEAPHY, SUSAN GRANT
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Publication number: 20110312057Abstract: A substrate or coating is provided that includes a lipase with enzymatic activity toward a component of a fingerprint. Also provided is a process for facilitating the removal of fingerprints is provided wherein an inventive substrate or coating including a lipase is capable of enzymatically degrading of one or more components of the fingerprint to facilitate fingerprint removal from the substrate or said coating. Applying heat to the substrate or coating increases the rate of fingerprint removal.Type: ApplicationFiled: June 21, 2010Publication date: December 22, 2011Applicants: Toyota Motor Engineering & Manufacturing North America, Inc., Regents of the University of Minnesota, Dept. of Bioproducts and Biosystems Engineering BioTechnolo, Toyota Motor CorporationInventors: Andreas Buthe, Ping Wang, Songtao Wu, Hongfei Jia, Masahiko Ishii, Minjuan Zhang
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Publication number: 20110312043Abstract: In an alcohol fermentation process, oil derived from biomass is hydrolyzed into an extractant available for in situ removal of a product alcohol such as butanol from a fermentation broth. The glycerides in the oil can be catalytically (e.g., enzymatically) hydrolyzed into free fatty acids, which form a fermentation product extractant having a partition coefficient for a product alcohol greater than a partition coefficient of the oil of the biomass for the product alcohol. Oil derived from a feedstock of an alcohol fermentation process can be hydrolyzed by contacting the feedstock including the oil with one or more enzymes whereby at least a portion of the oil is hydrolyzed into free fatty acids forming a fermentation product extractant, or the oil can be separated from the feedstock prior to the feedstock being fed to a fermentation vessel, and the separated oil can be contacted with the enzymes to form the fermentation product extractant.Type: ApplicationFiled: June 17, 2011Publication date: December 22, 2011Applicant: BUTAMAX(TM) ADVANCED BIOFUELS LLCInventors: KEITH H. BURLEW, ROBERT DICOSIMO, MICHAEL CHARLES GRADY
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Patent number: 8080400Abstract: A number of soluble engineered forms of MGLL that are suitable for high-throughput screening and protein crystallization, as well as a crystallized form of monoacylglycerol lipase protein (MGLL) and descriptions of the X-ray diffraction patterns are disclosed. The engineered constructs of MGLL permit the expression and purification of protein suitable for crystallography or high-throughput screening and identification of ligands, which can function as active agents to MGLL. The X-ray diffraction patterns allow the three dimensional structure of MGLL to be determined at atomic resolution so that ligand binding sites on MGLL can be identified and the interactions of ligands with MGLL amino acid residues can be modeled. Models prepared using such maps permit the design of ligands which can function as active agents which include, but are not limited to, those that function as inhibitors of MGLL.Type: GrantFiled: April 24, 2009Date of Patent: December 20, 2011Assignee: Janssen Pharmaceutica N.V.Inventors: Carsten Schubert, Bruce L. Grasberger, Celine Schalk-Hihi, Diane M. Maguire, Frank A. Lewandowski, Cynthia M. Milligan, Richard S. Alexander
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Patent number: 8076123Abstract: This invention relates to an oil degumming method that is free of emulsification. This method includes the steps of treating crude oil first with immobilized phospholipase and then extracting the phospholipase-treated crude oil with pure water or an aqueous solution.Type: GrantFiled: October 26, 2007Date of Patent: December 13, 2011Assignee: Oilseeds Biorefinery CorporationInventor: Chih-chung Chou
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Publication number: 20110300201Abstract: The invention provides methods, compositions, systems, and kits that include an enzyme/substrate co-delivery system. The liquid delivery system includes at least one enzyme encapsulated in a water-soluble polymeric matrix and a substrate for the enzyme in a carrier liquid in which the polymeric matrix is insoluble. When water is added, the polymeric matrix is solubilized and enzyme is released from the matrix, permitting catalytic action upon the substrate.Type: ApplicationFiled: November 3, 2009Publication date: December 8, 2011Applicant: DANISCO US INC.Inventors: Nathaniel T. Becker, Michael Stoner, Mee-Young Yoon
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Publication number: 20110296557Abstract: The present invention relates to isolated polypeptides having lipase activity and isolated polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides.Type: ApplicationFiled: December 9, 2009Publication date: December 1, 2011Applicant: Novozymes, Inc.Inventor: Michael Rey