Acting On Beta-1, 4-glucosidic Bond (e.g., Cellulase, Etc. (3.2.1.4)) Patents (Class 435/209)
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Patent number: 11624059Abstract: Detergent compositions may include endoglucanase variants and methods of using such detergent compositions. The endoglucanase may have an alteration at one or more positions where the variant has at least 60% but less than 100% sequence identity to SEQ ID NO: 2.Type: GrantFiled: August 3, 2018Date of Patent: April 11, 2023Assignee: Henkel AG & Co. KGaAInventors: Nina Mussmann, Susanne Wieland, Christian Degering, Rune Nygaard Monrad, Vasudeva P. Rao, Rajendra Kulothungan Sainathan, Sohel Dalal, Santhosh Vasu Mepadam, Allan Svendsen, Mette Louise Dissing Overgaard, Lars Anderson, Geetha Hiremath Mendez
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Patent number: 11384379Abstract: A method for producing an objective protein and a method for producing a disaccharide are provided. An objective protein is produced by culturing Talaromyces cellulolyticus in a culture medium containing an expression inducer such as gentiobiose. A disaccharide is produced from a saccharide raw material by enzymatic conversion using a disaccharide synthesizing enzyme.Type: GrantFiled: March 23, 2020Date of Patent: July 12, 2022Assignee: AJINOMOTO CO., INC.Inventors: Daiki Yahagi, Erika Yoshida, Hiroaki Fukada, Mitsunori Tokura, Uno Tagami, Masayuki Sugiki
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Patent number: 11365426Abstract: The present invention refers to a plant or plant part comprising a polynucleotide encoding a wildtype or mutated cellulose synthase (CESA) polypeptide, the expression of said polynucleotide confers to the plant or plant part tolerance to CESA-inhibiting herbicides, such as azines.Type: GrantFiled: March 20, 2020Date of Patent: June 21, 2022Assignee: BASF SEInventors: Stefan Tresch, Doreen Schachtschabel, Mihiret Tekeste Sisay, Jens Lerchl, Julia Major, Florian Vogt, Frederick Calo, Jill Marie Paulik
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Patent number: 11359188Abstract: The present invention relates to xanthan lyase variants and methods for obtaining xanthan lyase variants. The present invention also relates to polynucleotides encoding the variants; nucleic acid constructs, vectors, and host cells comprising the polynucleotides; and methods of using the variants.Type: GrantFiled: August 3, 2018Date of Patent: June 14, 2022Assignee: Novozymes A/SInventors: Rune Nygaard Monrad, Rajendra Kulothungan Sainathan, Geetha Hiremath Mendez, Sohel Dalal, Shilpi Agarwal, Allan Svendsen, Mette Louise Dissing Overgaard, Vasudeva Prahlada Rao
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Patent number: 11274122Abstract: The invention relates to a method for separating, from a culture medium, an enzymatic cocktail and the fungus Trichoderma Reesei, the culture medium resulting from enzyme production by the fungus, method in which: said culture medium is subjected, in a period of no longer than 30 h from the halting of production, to a separation on a filter press lined with a fabric having a porosity of 3-20 ?m, so as to obtain a filtrate having a corrected optical density OD at 600 nm of less than 2.5; and the liquid phase obtained is subjected to tangenital micro-filtration on a ceramic membrane having a cut-off limit of 0.5 to 1.4 ?m, so that the corrected OD at 600 nm does not exceed 0.1.Type: GrantFiled: July 11, 2017Date of Patent: March 15, 2022Assignees: IFP Energies Nouvelles, Institut National De La Recherche AgronomiqueInventors: Mohamed Fadhel Ben Chaabane, Romain Rousset
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Patent number: 11092609Abstract: The present invention is related to an in vitro method for diagnosing Gaucher's disease in a subject comprising a step of a) detecting a biomarker in a sample from the subject, wherein the biomarker is free lyso-Gb1.Type: GrantFiled: April 30, 2018Date of Patent: August 17, 2021Assignee: Centogene GmbHInventors: Arndt Rolfs, Hermann Mascher
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Patent number: 11053488Abstract: Disclosed herein are cellulase variants, or active fragments thereof, and polynucleotides encoding same, where the cellulase variants, or active fragments thereof, have endoglucanase activity. Also disclosed herein are compositions comprising the cellulase variants, or active fragments thereof; vectors and/or host cells comprising the polynucleotides encoding the cellulase variants, or active fragments thereof; and methods for making and/or using the cellulase variants, or active fragments thereof and/or compositions containing same; where the cellulase variants, or active fragments thereof, have endoglucanase activity.Type: GrantFiled: December 14, 2017Date of Patent: July 6, 2021Assignee: DANISCO US INCInventors: Richard Bott, David A. Estell, Neeraj Pandey, Sina Pricelius, Jian Yao
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Patent number: 11001827Abstract: The present disclosure relates to detergent compositions comprising xanthan lyase variants and methods for use of said compositions.Type: GrantFiled: August 24, 2017Date of Patent: May 11, 2021Assignee: Henkel AG & Co. KGaAInventors: Nina Mussmann, Susanne Wieland, Daniela Herbst, Lars Anderson, Jens Erik Nielsen, Allan Svendsen, Rune Nygaard Monrad, Rajendra Kulothungan Sainathan, Geetha Hiremath Mendez, Sohel Dalal, Shilpi Agarwal, Kasper D. Rand, Pernille F. Jensen
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Patent number: 10988747Abstract: The present disclosure relates to a detergent composition comprising endoglucanase variants and methods for use of said compositions.Type: GrantFiled: August 24, 2017Date of Patent: April 27, 2021Assignee: Henkel AG & Co. KGaAInventors: Nina Mussmann, Susanne Wieland, Daniela Herbst, Lars Anderson, Jens Erik Nielsen, Allan Svendsen, Rune Nygaard Monrad, Lars Giger, Vasudeva P. Rao, Sohel Dalal, Santhosh Vasu Mepadam, Marie T. Kruse, Kasper D. Rand, Pernille F. Jensen
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Patent number: 10907140Abstract: A mutant ?-glucosidase polypeptide exhibits enhanced thermostability and has the amino acid sequence: MAKFPRDFVWGTATSSYQIEGAVNEDGRTPSIWDTFSKTX1GKTYKGHT GDVACDHYHRYKEDVEILKEIGVKAYRFSIAWPRIFPEEGKYNPKGMDF YKKLIDELQKRDIX2PAATIYHWDLPQWAYDKGGGWLNRESIKWYVEYA TKLFEELGDAIPLWITHNEPWCSSILSYGIGEHAPGHKNYREALIAAHH ILLSHGEAVKAFREMNIKGSKIGITLNLTPAYPASEKEEDKLAAQYADG FANRWELDPIFKGNYPEDMMELYSKIIGEFDFIKEGDLETISVPIDFLG X3NYYTRSIVKYDEDSMLKAENVPGPGKRTEMGWEISPESLYDLLKRLD REYTKLPMYITENGAAFKDEVTEDGRVHDDERIEYIKEHLKAAAKFIGE GGNLKGYFVWSLMDNFEWAHGYSKRFGIVYVDYX4TQKRILKDSALWYK EVIX5DDGIED, wherein X1 is selected from E, P, T, M, A, S and G; X2 is selected from V, K, R and H; X3 is selected from I, L, M, P, T and A; X4 is selected from T, E, D, N, Q, M and P; and X5 is selected from L, R, K and H.Type: GrantFiled: September 5, 2016Date of Patent: February 2, 2021Assignee: Technische Universitaet MuenchenInventors: Vladimir Zverlov, Wolfgang Schwarz, Roman Prechtl, Benedikt Leis, Claudia Held, Wolfgang Liebl
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Patent number: 10808235Abstract: The application relates to a polypeptide having beta-glucosidase activity, its method of production and its uses.Type: GrantFiled: December 8, 2016Date of Patent: October 20, 2020Assignee: DSM IP Assets B.V.Inventor: Johannes Gustaaf Ernst Van Leeuwen
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Patent number: 10745679Abstract: The present invention relates to the expression and optimization of enzymes involved in the breakdown of lignocellulosic biomass. Disclosed are variants of the exoglucanase 1 of Trichoderma reesei, as well as the use of said variants with improved efficiency in methods for breaking down cellulose and for producing biofuel.Type: GrantFiled: June 12, 2015Date of Patent: August 18, 2020Assignees: IFP Energies Nouvelles, Centre National De La Recherche Scientifique—CNRS, ProteusInventors: Cecile Persillon, Christophe Ullmann, Celine Ayrinhac, Olivier Bonzom, Sebastien Fort, Sylvie Armand, Stephanie Pradeau, Antoine Margeot, Lionel Rousseau, Françoise Le Roux
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Patent number: 10738292Abstract: The present invention relates to a novel endoglucanase gene (GH5 family) from the soil metagenome. More specifically, this invention provides a recombinant plasmid and the recombinant hosts for the expression of novel gene sequence having cellulase activity. The cellulase in the invention has high specific activity towards ?-1,4 linkages in substrates such as carboxy-methyl cellulose and barley-?-glucan etc. This novel cellulase can have many industrial applications eg. food and feed industry, detergent, textile and biofuel industry etc.Type: GrantFiled: November 11, 2016Date of Patent: August 11, 2020Assignee: Council of Scientific & Industrial ResearchInventors: Roma Garg, Vijaya Brahma, Lata Verma, Girish Sahni
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Patent number: 10597673Abstract: The present invention refers to a plant or plant part comprising a polynucleotide encoding a wildtype or mutated cellulose synthase (CESA) polypeptide, the expression of said polynucleotide confers to the plant or plant part tolerance to CESA-inhibiting herbicides, such as azines.Type: GrantFiled: April 22, 2015Date of Patent: March 24, 2020Assignee: BASF SEInventors: Stefan Tresch, Doreen Schachtschabel, Mihiret Tekeste Sisay, Jens Lerchl, Julia Major, Florian Vogt, Frederick Calo, Jill Marie Paulik
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Patent number: 10538753Abstract: Disclosed herein are methods of making multifunctional microbial cellulases. The engineered multifunctional microbial cellulases disclosed herein exhibit improved activity over native cellulases.Type: GrantFiled: March 6, 2018Date of Patent: January 21, 2020Assignee: Alliance for Sustainably Enegry, LLCInventors: Roman Brunecky, Venkataramanan Subramanian, Stephen Robert Decker, Michael E. Himmel
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Patent number: 10526568Abstract: The present invention is directed to a cleaning composition including one or more Class III or Class IV BslA (Biofilm surface layer A) proteins and a surfactant system including one or more anionic surfactants and one or more co-surfactants, in a weight of ratio of less than 9:1. Methods of making and using such compositions are also provided.Type: GrantFiled: November 13, 2018Date of Patent: January 7, 2020Assignee: The Procter & Gamble CompanyInventors: Jean-Luc Philippe Bettiol, Denis Alfred Gonzales, Juan Esteban Velasquez, Nicholas William Geary
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Patent number: 10494622Abstract: The aim of the invention is to improve the secretion of a protein from a host cell in order to increase the product yield of protein in a fermentation process. This is achieved by an expression vector comprising a) a promoter sequence and b) a nucleic acid sequence that codes for a protein. The protein comprises a signal peptide and an additional amino acid sequence, and the signal peptide comprises an amino acid sequence that is at least 80% identical to the amino acid sequence specified in SEQ ID NO: 2, at least 80% identical to the amino acid sequence specified in SEQ ID NO: 4, at least 80% identical to the amino acid sequence specified in SEQ ID NO: 6, or the signal peptide comprises an amino acid sequence that is structurally homologous to at least one of said sequences.Type: GrantFiled: September 22, 2017Date of Patent: December 3, 2019Assignee: BASF SEInventors: Christian Degering, Thorsten Eggert, Stefan Evers, Karl-Heinz Maurer, Johannes Bongaerts
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Patent number: 10385324Abstract: The present invention relates to variants of cellobiohydrolase, preferably Cbh1, which have greater cellobiohydrolase activity. The invention also relates to a genetic construct, a host cell and to an enzyme composition comprising said variants. The invention further relates to a procedure for producing fermentable sugar and a procedure for producing a bioproduct, such as bioethanol, from cellulose material with the cellobiohydrolase variants, the host cell or the enzyme composition comprising said variants.Type: GrantFiled: April 8, 2016Date of Patent: August 20, 2019Assignee: ABENGOA BIOENERGÍA NUEVAS TECNOLOGÍAS, S.A.Inventors: Bruno Díez García, Noelia Valbuena Crespo, Francisco Manuel Reyes Sosa, Antonio Javier Moreno Pérez, Dolores Pérez Gómez, Ana Isabel Platero Gómez, Lucia Martín Pérez, Sandra Gavaldá Martín, Laura Viñas De La Cruz, Laura Sánchez Zamorano, Consolación Álvarez Núñez, María de los Ángeles Bermúdez Alcántara, Javier Rocha Martín, Laura Ledesma García, Juan Luis Ramos Martín, Laura Benítez Casanova, Macarena López Morales
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Patent number: 10385346Abstract: A xylosidase having improved enzymatic activity is disclosed. The amino acid sequence of the xylosidase is a modified amino acid sequence of SEQ ID NO: 2, wherein the modification is a substitution of phenylalanine at position 35 with glutamate, and/or a substitution of glutamine at position 41 with histidine.Type: GrantFiled: October 11, 2018Date of Patent: August 20, 2019Assignee: DONGGUAN APAC BIOTECHNOLOGY CO., LTD.Inventors: Ya-Shan Cheng, Tzu-Hui Wu, Cheng-Yen Lin, Hui-Lin Lai, Cheng-Bin Zheng, Ting-Yung Huang, I-Hsuan Lin, Jian-Wen Huang, Chun-Chi Chen, Rey-Ting Guo
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Patent number: 10174301Abstract: The present invention relates to isolated polypeptides having protease activity and isolated polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides in e.g. animal feed and detergents.Type: GrantFiled: December 20, 2012Date of Patent: January 8, 2019Assignee: Novozymes A/SInventors: Tine Hoff, Carsten Sjoeholm, Peter Rahbek Oestergaard, Katrine Pontoppidan
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Patent number: 10059932Abstract: An object is to provide a mutant of a cellulase-producing microorganism which produces a cellulase capable of preferentially producing a cello-oligosaccharide during the selective production of the cello-oligosaccharide through enzymolysis of a cellulosic material in the presence of the cellulase, a method for producing the cellulase and a method for producing a cello-oligosaccharide using the cellulase. The present invention relates to a mutant of a cellulase-producing microorganism, in which cellobiohydrolase and ?-glucosidase genes are disrupted.Type: GrantFiled: March 14, 2014Date of Patent: August 28, 2018Assignees: NAGAOKA UNIVERSITY OF TECHNOLOGY, INPEX CORPORATIONInventors: Wataru Ogasawara, Takashi Yamaguchi, Yosuke Shida, Yoshiro Ishii, Tatsuki Wakayama, Yoshiro Konda
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Patent number: 10041055Abstract: The present invention relates to polypeptides having mannanase activity, catalytic domains, and carbohydrate binding modules, and polynucleotides encoding the polypeptides, catalytic domains, and carbohydrate binding modules. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides, catalytic domains, and carbohydrate binding modules.Type: GrantFiled: September 19, 2014Date of Patent: August 7, 2018Assignee: NOVOZYMES A/SInventors: Kristian Bertel Roemer M. Krogh, Nikolaj Spodsberg, Klaus Gori, Pernille Von Freiesleben
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Patent number: 10006014Abstract: A novel insecticidal chitinase protein from fern Tectaria sp., a process for preparation of the insecticidal protein and nucleic acid sequence encoding for said insecticidal protein and its application for insect control purposes.Type: GrantFiled: March 29, 2016Date of Patent: June 26, 2018Assignee: Council of Scientific & Industrial ResearchInventors: Pradhyumna K. Singh, Santosh K. Upadhyay, Chandrashekar Krishnappa, Sharad Saurabh, Rahul Singh, Preeti Rai, Harpal Singh, Manisha Mishra, Ajit P. Singh, Praveen C. Verma, Kuttan P. Narayanan, Rakesh Tuli
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Patent number: 10006068Abstract: Disclosed herein is a pulverulent ceramide producing method that enables easily and efficiently extracting and separating ceramide from sugar beet pulp, and efficiently pulverizing the ceramide by spray drying. The pulverulent ceramide can be efficiently obtained by a process that includes concentrating, with and/or without adding water, a sugar beet pulp ethanol extract obtained by extraction of a sugar beet pulp (for example, such as a beet fiber) with ethanol, adding pectinase to the resulting concentrate and performing an enzymatic reaction, performing emulsification after inactivating the enzyme, and pulverizing the resulting emulsion using spray drying.Type: GrantFiled: July 29, 2014Date of Patent: June 26, 2018Assignees: Nippon Beet Sugar Manufacturing., Co., Ltd., T. HASEGAWA CO., LTD.Inventors: Keisuke Takahashi, Taizo Nagura, Hiroto Kikuchi, Teruhisa Ohashi, Natsumi Suzuki, Tadashi Yoshimoto, Tomohiro Hosogai
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Patent number: 9994836Abstract: The invention relates to novel variants of fungal endoglucanases, their production and means for their production. Especially the invention relates to variants of Acremonium thermophilum Cel45A. The invention further relates to enzyme preparations and detergent compositions comprising at least one novel variant endoglucanase as well as to processes for treating cellulosic material therewith. The novel variant endoglucanase polypeptides have improved performance in textile applications, especially in biofinishing and biostoning, and in detergent applications, in fabric care and color maintenance, especially in prevention and removal of fuzz and pills, in color care and revival.Type: GrantFiled: October 23, 2015Date of Patent: June 12, 2018Assignee: AB Enzymes OyInventors: Kari Juntunen, Marika Alapuranen, Leena Valtakari, Hanna-Mari Meriläinen, Terhi Puranen
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Patent number: 9969993Abstract: The present invention relates to cellulolytic compositions for degrading or converting cellulose-containing material and methods of producing and using the compositions.Type: GrantFiled: May 30, 2008Date of Patent: May 15, 2018Assignee: Novozymes, Inc.Inventors: Sandra Merino, Keith McFarland, Joel Cherry, Sarah Teter
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Patent number: 9926548Abstract: Polypeptides having endoglucanase activity, catalytic domains, and polynucleotides encoding the polypeptides or catalytic domains. Nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides or catalytic domains.Type: GrantFiled: October 23, 2014Date of Patent: March 27, 2018Assignee: Novozymes A/SInventors: Weijian Lai, Lan Tang, Marc Dominique Morant, Paul Harris
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Patent number: 9926547Abstract: The present invention relates to isolated polypeptides having endoglucanase activity, catalytic domains, cellulose binding domains and polynucleotides encoding the polypeptides, catalytic domains or cellulose binding domains. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides, catalytic domains or cellulose binding domains.Type: GrantFiled: April 26, 2012Date of Patent: March 27, 2018Assignee: Novozymes, Inc.Inventors: Nikolaj Spodsberg, Brett McBrayer, Pierre Cassland
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Patent number: 9771569Abstract: The present invention relates to isolated polypeptides having xylanase activity and isolated polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides.Type: GrantFiled: May 4, 2016Date of Patent: September 26, 2017Assignee: NOVOZYMES A/SInventors: Alfredo Lopez de Leon, Michael Rey
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Patent number: 9688974Abstract: An Aspergillus mutant strain characterized in that it is an auxotrophic mutant strain of Aspergillus oryzae strain AOK27L.Type: GrantFiled: January 20, 2015Date of Patent: June 27, 2017Assignee: HONDA MOTOR CO., LTD.Inventors: Satoru Shinkawa, Shigenobu Mitsuzawa, Maiko Tanaka
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Patent number: 9687531Abstract: Methods to introduce highly phosphorylated mannopyranosyl oligosaccharide derivatives containing mannose-6-phosphate (M6P), or other oligosaccharides bearing other terminal hexoses, to carbonyl groups on oxidized glycans of glycoproteins while retaining their biological activity are described. The methods are useful for modifying glycoproteins, including those produced by recombinant protein expression systems, to increase uptake by cell surface receptor-mediated mechanisms, thus improving their therapeutic efficacy in a variety of applications.Type: GrantFiled: August 20, 2014Date of Patent: June 27, 2017Assignee: Genzyme CorporationInventor: Yunxiang Zhu
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Patent number: 9683224Abstract: The present invention relates to new enzymes with improved properties and to compositions comprising these enzymes suitable for use in the production of a food, feed, or malt beverage product, such as in a brewing process.Type: GrantFiled: September 14, 2012Date of Patent: June 20, 2017Assignee: DuPont Nutrition Biosciences ApSInventors: Jens Frisbæk Sørensen, Lone Brønd Miller
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Patent number: 9663774Abstract: The present invention relates to isolated polypeptides having endoglucanase activity, catalytic domains, cellulose binding domains and polynucleotides encoding the polypeptides, catalytic domains or cellulose binding domains. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides, catalytic domains or cellulose binding domains.Type: GrantFiled: May 29, 2015Date of Patent: May 30, 2017Assignee: NOVOZYMES, INC.Inventors: Nikolaj Spodsberg, Tarana Shagasi
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Patent number: 9663808Abstract: The present invention relates to compositions comprising: a polypeptide having cellulolytic enhancing activity and an organic compound. The present invention also relates to methods of using the compositions.Type: GrantFiled: September 29, 2014Date of Patent: May 30, 2017Assignees: Novozymes, Inc., Novozymes A/SInventors: Jason Quinlan, Feng Xu, Matthew Sweeney, Katja Salomon Johansen
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Patent number: 9644223Abstract: A hyperthermostable endoglucanase including an endoglucanase catalytic domain, the endoglucanase catalytic domain including: (A) a polypeptide including an amino acid sequence represented by SEQ ID NO: 1; (B) a polypeptide including an amino acid sequence in which at least one amino acid is deleted, substituted, or added in the amino acid sequence represented by SEQ ID NO: 1, and having hydrolytic activity using p-nitrophenyl-?-D-cellobioside as a substrate at least under conditions of a temperature of 100° C. and a pH of 5.5; or (C) a polypeptide including an amino acid sequence having at least 70% sequence identity with the amino acid sequence represented by SEQ ID NO: 1, and having hydrolytic activity using p-nitrophenyl-?-D-cellobioside as a substrate at least under conditions of a temperature of 100° C. and a pH of 5.5.Type: GrantFiled: August 3, 2015Date of Patent: May 9, 2017Assignee: Honda Motor Co., Ltd.Inventors: Asuka Yamaguchi, Jiro Okuma, Yoshitsugu Hirose, Migiwa Suda, Yasuhiro Kondo, Tomohiko Kato, Daisuke Shibata
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Patent number: 9624518Abstract: The present invention relates to methods for degrading or converting a cellulosic material and for producing substances from the cellulosic material under high temperature conditions.Type: GrantFiled: April 27, 2012Date of Patent: April 18, 2017Assignee: Novozymes, Inc.Inventors: Tarana Shaghasi, Brett McBrayer
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Patent number: 9617528Abstract: Provided are isolated polypeptides having endoglucanase activity, catalytic domains, cellulose binding domains and polynucleotides encoding the polypeptides, catalytic domains or cellulose binding domains. Also provided are nucleic acid constructs, vectors and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides catalytic domains or cellulose binding domains.Type: GrantFiled: February 19, 2013Date of Patent: April 11, 2017Assignee: Novozymes A/SInventors: Weijian Lai, Nikolaj Spodsberg, Lars Anderson
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Patent number: 9593324Abstract: The present invention relates to the production of sugar hydrolysates from cellulosic material. The method may be used e.g. for producing fermentable sugars for the production of bioethanol from lignocellulosic material. Cellulolytic enzymes and their production by recombinant technology is described, as well as uses of the enzymes and enzyme preparations.Type: GrantFiled: October 3, 2013Date of Patent: March 14, 2017Assignee: Roal OyInventors: Jari Vehmaanperä, Marika Alapuranen, Terhi Puranen, Matti Siika-Aho, Jarno Kallio, Satu Jamsa, Sanni Voutilainen, Teemu Halonen, Liisa Viikari
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Patent number: 9562222Abstract: The present invention relates to isolated polypeptides having beta-glucosidase activity, beta-xylosidase activity, or beta-glucosidase and beta-xylosidase activity and isolated polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides.Type: GrantFiled: November 16, 2012Date of Patent: February 7, 2017Assignees: NOVOZYMES A/S, NOVOZYMES, INC.Inventor: Marc Morant
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Patent number: 9447400Abstract: The present disclosure is generally directed to enzymes and in particular beta-glucosidase variants. Also described are nucleic acids encoding beta-glucosidase variants, compositions comprising beta-glucosidase variants, methods of using beta-glucosidase variants, and methods of identifying additional useful beta-glucosidase variants.Type: GrantFiled: November 19, 2010Date of Patent: September 20, 2016Assignee: DANISCO US INC.Inventors: Richard R. Bott, Thijs Kaper, Bradley Kelemen, Frits Goedegebuur, Ronaldus Wilhelmus Hommes, Slavko Kralj, Paulien Kruithof, Igor Nikolaev, Wilhelmus Antonious Hendricus Van Der Kley, Johannes Franciscus Thomas Van Lieshout, Sander Van Stigt Thans, Gudrun Vogtentanz, Mats Sandgren
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Patent number: 9410165Abstract: The present invention relates to isolated polypeptides having cellulolytic enhancing activity and isolated polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods for producing and using the polypeptides.Type: GrantFiled: September 29, 2014Date of Patent: August 9, 2016Assignee: Novozymes, Inc.Inventors: Kimberly Brown, Paul Harris, Elizabeth Zaretsky, Edward Re, Elena Vlasenko, Keith McFarland, Alfredo Lopez de Leon
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Patent number: 9394555Abstract: The present invention relates to compositions comprising: a polypeptide having cellulolytic enhancing activity and a dioxy compound. The present invention also relates to methods of using the compositions.Type: GrantFiled: August 5, 2011Date of Patent: July 19, 2016Assignee: NOVOZYMES, INC.Inventors: Matthew Sweeney, Feng Xu, Jason Quinlan
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Patent number: 9353360Abstract: The present invention relates to isolated polypeptides having xylanase activity and isolated polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides.Type: GrantFiled: January 26, 2015Date of Patent: May 31, 2016Assignee: Novozymes, Inc.Inventors: Alfredo Lopez de Leon, Michael Rey
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Patent number: 9328456Abstract: The present invention relates to the method for manufacturing textile, by treating textile with an isolated polypeptide having endoglucanase activity, especially in biostoning and bio-polishing process.Type: GrantFiled: August 16, 2013Date of Patent: May 3, 2016Assignee: Novozymes A/SInventors: Weijian Lai, Lan Tang, Kirk Matthew Schnorr, Lars Anderson
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Patent number: 9295274Abstract: Methods for hydrolyzing okara using endopeptidase and/or endoglucanase enzymes are disclosed. The hydrolyzed okara has a diminished water-holding capacity and is useful for supplementing the protein and fiber content of food products.Type: GrantFiled: April 14, 2008Date of Patent: March 29, 2016Assignee: Delavau L.L.C.Inventors: Gregory E. Urbanski, Kevin W. Lang
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Patent number: 9267126Abstract: The present invention relates to isolated polypeptides having endoglucanase activity and isolated polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides.Type: GrantFiled: August 30, 2011Date of Patent: February 23, 2016Assignee: NOVOZYMES, INC.Inventor: Nikolaj Spodsberg
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Patent number: 9260705Abstract: The present invention relates to cellobiohydrolase variants having improved thermostability in comparison to wild-type CBH2a.Type: GrantFiled: December 31, 2014Date of Patent: February 16, 2016Assignee: Codexis, Inc.Inventors: Dipnath Baidyaroy, Louis Clark, David Elgart, Rama Voladri, Xiyun Zhang
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Patent number: 9249474Abstract: Methods for consolidated pretreatment and hydrolysis of genetically engineered plants expressing cell wall degrading enzymes are provided. Expression cassettes and vectors for making transgenic plants are described. Plants engineered to express one or more cell wall degrading enzymes using expression cassettes and vectors of the invention are also provided.Type: GrantFiled: March 7, 2012Date of Patent: February 2, 2016Assignee: Agrivida, Inc.Inventors: R. Michael Raab, Dongcheng Zhang, Oleg Bougri
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Patent number: 9238806Abstract: The present invention relates to a depilling composition comprising an enzyme mixture that comprises a Family 45 cellulase and one or more additional cellulases selected from a Family 5 cellulase, a Family 6 cellulase or a combination thereof. The enzyme mixture may be characterized in that the Family 45 and Family 5 cellulases or the Family 45 and Family 6 cellulases are present at a weight ratio that exhibits synergy in an assay that measures specific depilling activity. The enzyme mixture may be secreted by a genetically modified microbe overexpressing the foregoing cellulases. Also provided is a process for depilling that comprises a step of contacting cellulose-containing goods with the depilling composition.Type: GrantFiled: February 9, 2012Date of Patent: January 19, 2016Assignee: Novozymes A/SInventors: Christopher Hill, John J. Tomashek, Loreta Gudynaite-Savitch, Guylaine Therrien, Sophie Calixte
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Patent number: 9234216Abstract: In alternative embodiments, the invention provides polypeptides having a lignocellulolytic (lignocellulosic) activity, e.g., a ligninolytic and cellulolytic activity, including, e.g., a glycosyl hydrolase, a cellulase, an endoglucanase, a cellobiohydrolase (cbhl) (e.g., an exo-cellobiohydrolase, e.g., having an “exo” activity that can processively release cellobiose units ?-1,4 glucose-glucose disaccharide), a beta-glucosidase, a xylanase, a mannanse, a xylosidase (e.g., a (?-xylosidase) and/or an arabinofuranosidase activity, polynucleotides encoding these polypeptides, and methods of making and using these polynucleotides and polypeptides. In one embodiment, the invention provides thermostable and thermotolerant forms of polypeptides of the invention. The polypeptides and nucleic acids of the invention are used in a variety of pharmaceutical, agricultural and industrial contexts; for example, as enzymes for the bioconversion of a biomass, e.g.Type: GrantFiled: October 6, 2011Date of Patent: January 12, 2016Assignee: BP Corporation North America Inc.Inventors: Justin T. Stege, Alexander Varvak, John Poland, Chris S. Lyon, Shaun Healey, Peter Luginbuhl