Acting On Beta-1, 4-glucosidic Bond (e.g., Cellulase, Etc. (3.2.1.4)) Patents (Class 435/209)
  • ENZYMES FOR THE TREATMENT OF LIGNOCELLULOSICS, NUCLEIC ACIDS ENCODING THEM AND METHODS FOR MAKING AND USING THEM
    Publication number: 20140223602
    Abstract: The invention provides polypeptides having a lignocellulolytic activity, e.g., a glycosyl hydrolase, a cellulase, an endoglucanase, a cellobiohydrolase, a beta-glucosidase, a xylanase, a mannanse, a xylosidase (e.g., a ?-xylosidase), an arabinofuranosidase, and/or a glucose oxidase activity, polynucleotides encoding these polypeptides, and methods of making and using these polynucleotides and polypeptides. In one aspect, the invention provides polypeptides that can enzymatically process (hydrolyze) sugarcane bagasse, i.e., for sugarcane bagasse degradation, or for biomass processing, and polynucleotides encoding these enzymes, and making and using these polynucleotides and polypeptides. In one embodiment, the invention provides thermostable and thermotolerant forms of polypeptides of the invention.
    Type: Application
    Filed: September 27, 2013
    Publication date: August 7, 2014
    Applicant: BP Corporation North America LLC
    Inventors: Cathy Chang, Justin T. Stege, Nahla M. Aboushadi, Gordana Djordjevic, Ellen G. Burke, Peter Luginbuhl, Mark Dycaico, Toby H. Richardson, John Poland, Hefner Ying, Stacy M. Miles
  • Surfactant tolerant cellulase and method for modification thereof
    Patent number: 8796005
    Abstract: A method for suppressing a reduction in an endoglucanase activity in the presence of a surfactant, characterized by modifying a protein having the endoglucanase activity in which the N-terminus is an amino acid other than pyroglutamic acid, to a protein having the N-terminus of pyroglutamic acid, is disclosed. Further, a modified protein having an endoglucanase activity wherein the N-terminal amino acid is converted into pyroglutamic acid by an amino acid modification, a polynucleotide encoding the protein, an expression vector comprising the polynucleotide, a host cell transformed with the expression vector, and a process for producing the protein by cultivating the host cell, are disclosed.
    Type: Grant
    Filed: September 19, 2013
    Date of Patent: August 5, 2014
    Assignee: Meiji Seika Pharma Co., Ltd.
    Inventors: Manabu Watanabe, Koji Yanai, Yumiko Tsuyuki
  • ENDOGENOUS DNASE ACTIVITY TO REDUCE DNA CONTENT
    Publication number: 20140212885
    Abstract: The application provides a method of reducing the DNA content of a protein preparation or a culture broth from a filamentous fungal host cell using an endogenous filamentous fungal host DNase activity.
    Type: Application
    Filed: September 20, 2012
    Publication date: July 31, 2014
    Applicant: Danisco US Inc.
    Inventors: Katherine M. Hoffmann, Douglas Ko, Michael Ward
  • Mutant cellobiohydrolase
    Patent number: 8790894
    Abstract: The invention relates to Mutant cellobiohydrolase, being a mutant of SEQ ID NO:1, having a substitution at position N247(I,F,H,W) of SEQ ID NO: 1, wherein the mutant cellobiohydrolase has at least 50% sequence identity with SEQ ID NO: 1, and wherein the mutant cellobiohydrolase has CBHI activity.
    Type: Grant
    Filed: January 30, 2012
    Date of Patent: July 29, 2014
    Assignee: DSM IP Assets B.V.
    Inventors: Jan Metske Van Der Laan, Margot Elisabeth Francoise Schooneveld-Bergmans, Denise Ilse Jacobs
  • Methods for Obtaining Positive Transformants of a Filamentous Fungal Host Cell
    Publication number: 20140206086
    Abstract: The present invention relates to methods for obtaining positive transformants of a filamentous fungal host cell, comprising: transforming a tandem construct into a population of cells of the filamentous fungal host a tandem construct and isolating a transformant of the filamentous fungal host cell comprising the tandem construct. The present invention also relates to such tandem constructs, filamentous fungal host cells comprising such tandem constructs, and methods of producing multiple recombinant proteins.
    Type: Application
    Filed: August 23, 2012
    Publication date: July 24, 2014
    Applicant: Novozymes, Inc.
    Inventor: Donna Moyer
  • Variant CBH2 cellulases and related polynucleotides
    Patent number: 8785170
    Abstract: The invention provides variants of a Streptomyces sp. CBH2 that have improved properties compared to the wild type enzyme and methods of using the variants in the hydrolysis of substrates comprising cellulose.
    Type: Grant
    Filed: August 31, 2010
    Date of Patent: July 22, 2014
    Assignee: Codexis, Inc.
    Inventors: Ish K. Dhawan, Erika N. Segraves
  • Extracellular secretion of recombinant proteins
    Patent number: 8785159
    Abstract: Nucleic acids encoding secretion signals, expression vectors containing the nucleic acids, and host cells containing the expression vectors are disclosed. Also disclosed are polypeptides that contain the secretion signals and methods of producing polypeptides, including methods of directing the extracellular secretion of the polypeptides. Exemplary embodiments include cellulase proteins fused to secretion signals, methods to produce and isolate these polypeptides, and methods to degrade lignocellulosic biomass.
    Type: Grant
    Filed: November 19, 2010
    Date of Patent: July 22, 2014
    Assignee: Alliance for Sustainable Energy, LLC
    Inventors: Jeffrey G. Linger, Aldis Darzins
  • GLUCANASES, NUCLEIC ACIDS ENCODING THEM AND METHODS FOR MAKING AND USING THEM
    Publication number: 20140199705
    Abstract: The invention relates to polypeptides having glucanase, e.g., endoglucanase, mannanase, xylanase activity or a combination of these activities, and polynucleotides encoding them. In one aspect, the glucanase activity is an endoglucanase activity (e.g., endo-1,4-beta-D-glucan 4-glucano hydrolase activity) and comprises hydrolysis of 1,4-beta-D-glycosidic linkages in cellulose, cellulose derivatives (e.g., carboxy methyl cellulose and hydroxy ethyl cellulose) lichenin, beta-1,4 bonds in mixed beta-1,3 glucans, such as cereal beta-D-glucans or xyloglucans and other plant material containing cellulosic parts. In addition, methods of designing new enzymes and methods of use thereof are also provided. In alternative aspects, the new glucanases e.g., endoglucanases, mannanases, xylanases have increased activity and stability at increased pH and temperature.
    Type: Application
    Filed: September 5, 2013
    Publication date: July 17, 2014
    Applicants: SYNGENTA PARTICIPATIONS AG, BP CORPORATION NORTH AMERICA INC.
    Inventors: Brian Steer, Walter Niles Callen, Shaun Healey, Derrick Pulliam
  • Polypeptides Having Beta-Glucosidase Activity, Beta-Xylosidase Activity, or Beta-Glucosidase and Beta-Xylosidase Activity and Polynucleotides Encoding Same
    Publication number: 20140201871
    Abstract: The present invention relates to isolated polypeptides having beta-glucosidase activity, beta-xylosidase activity, or beta-glucosidase and beta-xylosidase activity and isolated polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides.
    Type: Application
    Filed: March 25, 2014
    Publication date: July 17, 2014
    Applicant: Novozymes Inc.
    Inventor: Marc Dominique Morant
  • Polypeptides having endoglucanase activity and polynucleotides encoding same
    Patent number: 8778639
    Abstract: The present invention relates to isolated polypeptides having endoglucanase activity and polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides.
    Type: Grant
    Filed: February 12, 2013
    Date of Patent: July 15, 2014
    Assignee: Novozymes Inc.
    Inventor: Nikolaj Spodsberg
  • Polypeptides having cellobiohydrolase activity and polynucleotides encoding same
    Patent number: 8778641
    Abstract: The present invention relates to isolated polypeptides having cellobiohydrolase activity and polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides.
    Type: Grant
    Filed: February 12, 2013
    Date of Patent: July 15, 2014
    Assignee: Novozymes Inc.
    Inventor: Nikolaj Spodsberg
  • Polypeptides having cellobiohydrolase activity and polynucleotides encoding same
    Patent number: 8778640
    Abstract: The present invention relates to isolated polypeptides having cellobiohydrolase activity and polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides.
    Type: Grant
    Filed: February 12, 2013
    Date of Patent: July 15, 2014
    Assignee: Novozymes Inc.
    Inventor: Nikolaj Spodsberg
  • Polypeptides having endoglucanse activity and polynucleotides encoding same
    Patent number: 8771993
    Abstract: The present invention relates to isolated polypeptides having endoglucanase activity and polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides.
    Type: Grant
    Filed: February 12, 2013
    Date of Patent: July 8, 2014
    Assignees: Novozymes A/S, Novozymes Inc.
    Inventor: Nikolaj Spodsberg
  • Polypeptides having cellobiohydrolase activity and polynucleotides encoding same
    Patent number: 8771994
    Abstract: The present invention relates to isolated polypeptides having cellobiohydrolase activity and polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides.
    Type: Grant
    Filed: February 12, 2013
    Date of Patent: July 8, 2014
    Assignee: Novozymes Inc.
    Inventor: Nikolaj Spodsberg
  • ?-Glucosidase variants
    Patent number: 8772010
    Abstract: The invention relates to recombinantly produced ?-Glucosidase Variants with enhanced thermoactivity compared to naturally occurring proteins. The invention also provides methods for producing a variant ?-glucosidase polypeptide with improved thermoactivity by identifying performance sensitive positions in a target ?-glucosidase polypeptide and substituting the residue at that position with a thermoactivity enhancing residue.
    Type: Grant
    Filed: June 16, 2010
    Date of Patent: July 8, 2014
    Assignee: Codexis, Inc.
    Inventors: Xiyun Zhang, Jie Yang, Dipnath Baidyaroy, Sally Rhiannon Postlethwaite, Attila Andor, Louis Clark, Lisa M. Newman
  • Alcohol product processes
    Patent number: 8772001
    Abstract: Processes for production of an alcohol product from granular starch comprising a pre-treatment at an elevated temperature below the initial gelatinization temperature of the granular starch followed by simultaneous saccharification and fermentation, and optionally recovery of ethanol, are described.
    Type: Grant
    Filed: June 13, 2008
    Date of Patent: July 8, 2014
    Assignee: Novozymes A/S
    Inventors: Hans Sejr Olsen, Sven Pedersen, Rikke Monica Festersen
  • TRANSFORMANT OF YEAST OF GENUS SCHIZOSACCHAROMYCES, METHOD FOR PRODUCING SAME, METHOD FOR PRODUCING ß-GLUCOSIDASE, AND CELLULOSE DEGRADATION METHOD
    Publication number: 20140186897
    Abstract: To provide a transformant of a yeast of the genus Schizosaccharomyces which can produce a modified-type ?-glucosidase with improved glucosidase activity, a method for producing such a transformant, a method for producing the modified-type ?-glucosidase, and a cellulose degradation method using the modified-type ?-glucosidase.
    Type: Application
    Filed: September 19, 2013
    Publication date: July 3, 2014
    Applicant: ASAHI GLASS COMPANY, LIMITED
    Inventors: Katsunori OKADA, Hideki Tohda
  • Process for production of an enzyme product
    Patent number: 8765442
    Abstract: The invention relates to a process for production of an enzyme product having a plurality of enzyme activities obtained by fermentation of an Aspergillus strain.
    Type: Grant
    Filed: December 21, 2009
    Date of Patent: July 1, 2014
    Assignee: DuPont Nutrition Biosciences ApS
    Inventor: Mette Zacho Grønfeldt
  • CBH1 homologs and varian CBH1 cellulase
    Patent number: 8765440
    Abstract: Disclosed are a number of homologs and variants of Hypocrea jecorina Cel7A (formerly Trichoderma reesei cellobiohydrolase I or CBH1), nucleic acids encoding the same and methods for producing the same. The homologs and variant cellulases have the amino acid sequence of a glycosyl hydrolase of family 7A wherein one or more amino acid residues are substituted and/or deleted.
    Type: Grant
    Filed: March 25, 2011
    Date of Patent: July 1, 2014
    Assignee: Danisco US Inc.
    Inventors: Frits Goedegebuur, Peter Gualfetti, Colin Mitchinson, Paulien Neefe
  • ENHANCED HETEROLOGOUS PROTEIN PRODUCTION IN KLUYVEROMYCES MARXIANUS
    Publication number: 20140178933
    Abstract: An expression vector which is capable of overexpressing a protein of interest in a host cell, a host cell comprising the expression vector, and a method of producing a protein of interest are provided.
    Type: Application
    Filed: April 30, 2012
    Publication date: June 26, 2014
    Applicants: RESEARCH & BUSINESS FOUNDATION SUNGKYUNKWAN UNIVERSITY, Samsung Electronics Co., Ltd.
    Inventors: Hyun Min Koo, Byung Jo Yu, Ki Sung Lee, Jae Chan Park, Dae Hyuk Kweon, Sung Minm Park
  • CELLULASE COMPOSITION CONTAINING CELLULASE AND PAPERMAKING POLYMERS FOR PAPER DRY STRENGTH APPLICATION
    Publication number: 20140174685
    Abstract: Disclosed herein are cellulase compositions useful as papermaking performance additives for improving paper dry strength of a paper product and reducing refining energy in papermaking processes, and improving paper production. These cellulase compositions are formulated using cellulase, papermaking contaminant control polymers, protein stabilizers and cellulase enhancers. These cellulase compositions measure higher in endo-cellulase activity with better stability than conventional cellulase, and have shown differentiating performance in improving paper dry strength properties versus cellulase alone.
    Type: Application
    Filed: October 8, 2013
    Publication date: June 26, 2014
    Applicant: HERCULES INCORPORATED
    Inventors: Qu-Ming Gu, Frank J. Sutman
  • STABILIZED CHLORINE DIOXIDE FOR CONTAMINATION CONTROL IN ZYMOMONAS FERMENTATION
    Publication number: 20140178956
    Abstract: Though chlorine dioxide is generally used to control bacterial contamination, a method was developed which allows the use of stabilized chlorine dioxide (SCD) for controlling contamination during fermentation that uses the bacteria Zymomonas as the biocatalyst, even though Zymomonas is sensitive to chlorine dioxide. Parameters were identified for inoculating a composition for fermentation with Zymomonas cells after a time period has elapsed following SCD addition.
    Type: Application
    Filed: December 20, 2012
    Publication date: June 26, 2014
    Applicant: E I DU PONT DE NEMOURS AND COMPANY
    Inventors: Maria C. Leana, Brian G. Lefebvre
  • Polypeptides Having Cellulase Activity and Polynucleotides Encoding Same
    Publication number: 20140179588
    Abstract: The present invention relates to isolated polypeptides having cellulase activity. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides.
    Type: Application
    Filed: August 10, 2012
    Publication date: June 26, 2014
    Applicant: Novozymes A/S
    Inventors: Kirk Matthew Schnorr, Lars Anderson, Maria Leonor Quintais Cancela Da Fonseca, Ricardo Leite
  • MUTANT ENDOGLUCANASE
    Publication number: 20140178947
    Abstract: Endoglucanase characterized by a decreased degree of activity inhibition by a lignin-derived aromatic compound, and prepared by substituting tryptophan at position 273 in the amino acid sequence of wild-type thermophilic bacterium-derived endoglucanase with an amino acid other than aromatic amino acids.
    Type: Application
    Filed: March 4, 2014
    Publication date: June 26, 2014
    Applicants: National Institute of Advanced Industrial Science and Technology, Toray Industries, Inc.
    Inventors: Hiroyuki Kurihara, Takeshi Tsukada, Katsushige Yamada, Yumiko Mishima, Yuka Maeno, Kazuhiko Ishikawa
  • CSNR-deficient actinobacteria for the production of an enzyme having chitosanase activity
    Patent number: 8759033
    Abstract: The present invention relates to genetically modified actinobacteria for the production of an enzyme having chitosanase activity. The genetically modified actinobacteria have a reduced (or abolished) activity of the CsnR polypeptide. Such reduced activity can be obtained by reducing the capacity of expressing the csnR gene, its corresponding transcript or expressing a dominant-negative CsnR polypeptide. Such genetically modified actinobacteria are less dependent (and, in some embodiment, totally independent) on the presence of chitosan in the culture medium for producing an enzyme having chitosanase activity. In addition, the genetically modified bacteria produce less proteases in the culture medium and ultimately provide a chitosanase end-product with higher purity.
    Type: Grant
    Filed: February 8, 2013
    Date of Patent: June 24, 2014
    Assignee: Socpra Sciences et Genie S.E.C.
    Inventors: Ryszard Brzezinski, Marie-Pierre Dubeau
  • Cellobiohydrolase variants
    Patent number: 8759064
    Abstract: The invention relates to recombinant expression of variant forms of C1 CBH1a and homologs thereof, having improved thermostability, low-pH tolerance, specific activity and other desirable properties. Also provided are methods for producing ethanol and other valuable organic compounds by combining cellobiohydrolase variants with cellulosic materials.
    Type: Grant
    Filed: May 13, 2011
    Date of Patent: June 24, 2014
    Assignee: Codexis, Inc.
    Inventors: Vesna Mitchell, Grzegorz Wojciechowski, Oscar Alvizo
  • Process for the hydrolysis of cellulose
    Patent number: 8758517
    Abstract: There is disclosed a process for the hydrolysis of cellulose comprising the sequential steps (a) mixing cellulose with a viscosity below 900 ml/g with an aqueous solution to obtain a liquid, wherein particles comprising cellulose in said liquid have a diameter of maximum 200 nm, wherein the temperature of the aqueous solution is below 35° C., and wherein the pH of the aqueous solution is above 12, (b) subjecting the liquid to at least one of the steps: (i) decreasing the pH of the liquid with at least 1 pH unit and (ii) increasing the temperature by at least 20° C., and (c) hydrolyzing the cellulose. Moreover there is disclose glucose manufactured according to the method and ethanol manufactured from the glucose. Advantages include that the cellulose is hydrolyzed faster and to a greater extent after the treatment. The yield is increased.
    Type: Grant
    Filed: April 16, 2010
    Date of Patent: June 24, 2014
    Assignee: Re:Newcell Lux S.a.r.l
    Inventors: Gunnar Henriksson, Mikael Lindstrom
  • Polypeptides having xylanase activity and polynucleotides encoding same
    Patent number: 8759041
    Abstract: The present invention relates to isolated polypeptides having xylanase activity and polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides.
    Type: Grant
    Filed: February 12, 2013
    Date of Patent: June 24, 2014
    Assignee: Novozymes Inc.
    Inventor: Nikolaj Spodsberg
  • Polypeptides having cellobiohydrolase activity and polynucleotides encoding same
    Patent number: 8759040
    Abstract: The present invention relates to isolated polypeptides having cellobiohydrolase activity and polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides.
    Type: Grant
    Filed: February 12, 2013
    Date of Patent: June 24, 2014
    Assignee: Novozymes Inc.
    Inventor: Nikolaj Spodsberg
  • Polypeptides having cellobiohydrolase activity and polynucleotides encoding same
    Patent number: 8759023
    Abstract: The present invention relates to isolated polypeptides having cellobiohydrolase activity and polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides.
    Type: Grant
    Filed: January 26, 2012
    Date of Patent: June 24, 2014
    Assignee: Novozymes Inc.
    Inventor: Nikolaj Spodsberg
  • Mashing Process
    Publication number: 20140170712
    Abstract: The present invention relates to a mashing and filtration step in a brewing process and to a composition useful in the mashing and filtration step of a brewing process.
    Type: Application
    Filed: February 27, 2014
    Publication date: June 19, 2014
    Applicant: Novozymes A/S
    Inventors: Rikke Monica Festersen, Anders Viksoe Nielsen, Christel Thea Joergensen, Lars Lehmann Hylling Christensen
  • Polypeptides having cellobiohydrolase activity and polynucleotides encoding same
    Patent number: 8753860
    Abstract: The present invention relates to isolated polypeptides having cellobiohydrolase activity and isolated polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides.
    Type: Grant
    Filed: February 12, 2013
    Date of Patent: June 17, 2014
    Assignee: Novozymes A/S
    Inventors: Marc D. Morant, Paul Harris
  • MODIFIED GLUCOSIDASE, BETA, ACID POLYNUCLEOTIDES FOR TREATING PROTEIN DEFICIENCY
    Publication number: 20140155473
    Abstract: The invention relates to compositions and methods for the preparation, manufacture and therapeutic use of polynucleotides, primary transcripts and mmRNA molecules.
    Type: Application
    Filed: February 3, 2014
    Publication date: June 5, 2014
    Applicant: Moderna Threrapeutics, Inc.
    Inventors: Stephane Bancel, Tirtha Chakraborty, Antonin de Fougerolles, Susan Whoriskey
  • STRAIN OF BACILLUS SUBTILIS AND APPLICATIONS THEREOF
    Publication number: 20140154750
    Abstract: The present invention is directed to a strain of Bacillus subtilis and applications using the strain to raise the efficiency and/or yield of generation of glucose produced by the hydrolysis of cellulose.
    Type: Application
    Filed: May 30, 2013
    Publication date: June 5, 2014
    Applicant: NATIONAL CENTRAL UNIVERSITY
    Inventors: SHIR-LY HUANG, SHENG-HSIN CHOU
  • GLYCOSIDE HYDROLASES HAVING MULTIPLE HYDROLASE ACTIVITIES
    Publication number: 20140154752
    Abstract: Glycoside hydrolases having at least two different hydrolytic activities are provided. In one embodiment, an isolated recombinant hydrolase having at least two activities selected from a group including asparagine derivatives, glutamine derivatives, and histidine derivatives is provided. Further, a method of generating free sugars from a mixture comprising asparagine derivatives, glutamine derivatives, and histidine derivatives is provided.
    Type: Application
    Filed: May 1, 2012
    Publication date: June 5, 2014
    Applicants: Sandia Corporation, The Regents of the University of California
    Inventors: Zhiwei Chen, Gregory D. Friedland, Swapnil R. Chhabra, Dylan C. Chivian, Blake A. Simmons
  • Human lysosomal proteins from plant cell culture
    Patent number: 8741620
    Abstract: A device, system and method for producing glycosylated proteins in plant culture, particularly proteins having a high mannose glycosylation, while targeting such proteins with an ER signal and/or by-passing the Golgi. The invention further relates to vectors and methods for expression and production of enzymatically active high mannose lysosomal enzymes using transgenic plant root, particularly carrot cells. More particularly, the invention relates to host cells, particularly transgenic suspended carrot cells, vectors and methods for high yield expression and production of biologically active high mannose Glucocerebrosidase (GCD). The invention further provides for compositions and methods for the treatment of lysosomal storage diseases.
    Type: Grant
    Filed: July 23, 2012
    Date of Patent: June 3, 2014
    Assignee: Protalix Ltd.
    Inventors: Yoseph Shaaltiel, Gideon Baum, Daniel Bartfeld, Sharon Hashmueli, Ayala Lewkowicz
  • Polypeptides Having Endoglucanase Activity And Polynucleotides Encoding Same
    Publication number: 20140150137
    Abstract: The present invention relates to isolated polypeptides having endoglucanase activity, catalytic domains, cellulose binding domains and polynucleotides encoding the polypeptides, catalytic domains or cellulose binding domains. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides, catalytic domains or cellulose binding domains.
    Type: Application
    Filed: April 26, 2012
    Publication date: May 29, 2014
    Applicant: Novozymes, Inc.
    Inventors: Nikolaj Spodsberg, Brett McBrayer, Pierre Cassland
  • VARIANT CBH I POLYPEPTIDES WITH REDUCED PRODUCT INHIBITION
    Publication number: 20140147894
    Abstract: The present disclosure relates to variant CBH I polypeptides that have reduced product inhibition, and compositions, e.g., cellulase compositions, comprising variant CBH I polypeptides. The variant CBH I polypeptides and related compositions can be used in variety of agricultural and industrial applications. The present disclosure further relates to nucleic acids encoding variant CBH I polypeptides and host cells that recombinantly express the variant CBH I polypeptides.
    Type: Application
    Filed: October 6, 2011
    Publication date: May 29, 2014
    Applicant: BP Corporation North America Inc.
    Inventors: Justin T. Stege, Alexander Varvak, John Poland, Chris S. Lyon, Shaun Healey, Peter Luginhuhl
  • Cellulose and ligno-cellulose active proteins
    Patent number: 8735100
    Abstract: Methods to digest carbohydrates, especially lignocelluloses and hemicelluloses, using fungal proteins previously not recognized as having this activity are described.
    Type: Grant
    Filed: March 17, 2010
    Date of Patent: May 27, 2014
    Assignee: Nederlandse Organisatie Voor Toegepast -Natuurwetenschappelijk Onderzoek TNO
    Inventor: Peter Jan Punt
  • Methods for Enhancing the Degradation of Cellulosic Material with Chitin Binding Proteins
    Publication number: 20140141471
    Abstract: The present invention relates to methods for degrading or converting a cellulosic material and for producing substances from the cellulosic material.
    Type: Application
    Filed: May 18, 2012
    Publication date: May 22, 2014
    Applicant: Novozymes, Inc.
    Inventors: Feng Xu, William Widner, Ani Tejirian
  • YEAST CELL CAPABLE OF CONVERTING SUGARS INCLUDING ARABINOSE AND XLOSE
    Publication number: 20140141473
    Abstract: Yeast cell belonging to the genus Saccharomyces having introduced into its genome at least one xylA gene and at least one of each of araA, araB and araD genes and that is capable of consuming a mixed sugar mixture comprising glucose, xylose and arabinose, wherein the cell co-consumes glucose and arabinose, has genetic variations obtained during adaptive evolution and has a specific xylose consumption rate in the presence of glucose that is 0.25 g xylose/h, g DM or more.
    Type: Application
    Filed: April 20, 2012
    Publication date: May 22, 2014
    Applicant: DSM IP ASSETS B.V.
    Inventors: Paul Klaassen, Bianca Elisabeth Maria Gielesen, Gijsberdina Pieternella Van Suylekom, Panagiotis Sarantinopoulos, Wilbert Herman Marie Heijne, Aldo Greeve
  • METHOD FOR REDUCING VISCOSITY IN SACCHARIFICATION PROCESS
    Publication number: 20140134677
    Abstract: The present invention relates to compositions that can be used in hydrolyzing biomass such as compositions comprising a polypeptide having glycosyl hydrolase family 61/endoglucanase activity, methods for hydrolyzing biomass material, and methods for reducing viscosity of biomass mixture using a composition comprising a polypeptide having glycosyl hydrolase family 61/endoglucanase activity.
    Type: Application
    Filed: March 16, 2012
    Publication date: May 15, 2014
    Applicant: DANISCO US INC.
    Inventors: Colin Mitchinson, Mian Li, Bradley R. Kelemen, Suzanne E. Lantz, Keith D. Wing, William D. Hitz
  • Xylanase having improved enzymatic activity
    Patent number: 8722382
    Abstract: A xylanase having increased enzymaic activity is disclosed. The xylanase has a modified amino acid sequence of SEQ ID NO: 2, wherein Tryptophan at position 125 is substituted with Phenylalanine and Phenylalanine at position 163 is substituted with Tryptophan.
    Type: Grant
    Filed: September 27, 2013
    Date of Patent: May 13, 2014
    Assignee: Dongguan APAC Biotechnology Co., Ltd.
    Inventors: Rey-Ting Guo, Ya-Shan Cheng, Jian-Wen Huang, Tzu-Hui Wu, Hui-Lin Lai, Cheng-Yen Lin, Ting-Yung Huang
  • MICROPELLET COMPOSITIONS COMPRISING PANCREATIN CONTAINING DIGESTIVE ENZYME MIXTURE
    Publication number: 20140127307
    Abstract: The present invention relates to a small particle size composition comprising pancreatin containing digestive enzymes for use in patients in need, including pediatric, geriatric, and adult patients, particularly those patients with dysphagia or wherein enteral administration using such composition would be suitable. In addition, the invention is directed to the composition as particles, such as micropellets or microgranules having a high potency, high useable yield and at least 10%-90% of 400-800 ?m. Furthermore, the composition optionally has an improved enteric coating and concomitant improved stability and enzyme activity compared to conventional prepared enterically coated pancreatic enzyme particles.
    Type: Application
    Filed: November 2, 2012
    Publication date: May 8, 2014
    Applicant: APTALIS PHARMA LIMITED
    Inventors: Gopi M. VENKATESH, Craig Kramer, Flavio Fabiani, Luigi Mapelli, Giovanni Ortenzi, Massimo Latino
  • MUTANT B-GLUCOSIDASE, ENZYME COMPOSITION FOR DECOMPOSING BIOMASS, AND METHOD OF PRODUCING SUGAR SOLUTION
    Publication number: 20140127759
    Abstract: A ?-glucosidase exhibits high activity in the presence of biomass and has high thermal stability compared to conventional enzymes. The ?-glucosidase includes substitutions and/or deletions of amino acids, at least three amino acids selected from the group consisting of Glu39, Asp169, Arg170, Arg220, Tyr227, and Glu330, of the parent ?-glucosidase with other amino acids and exhibits a decomposition activity.
    Type: Application
    Filed: January 14, 2014
    Publication date: May 8, 2014
    Applicant: Toray Industries, Inc.
    Inventors: Hiroyuki Kurihara, Katsushige Yamada, Kazuhiko Ishikawa, Yumiko Mishima, Yasunobu Wada, Yuji Kado
  • Variant CBH2 cellulases
    Patent number: 8715997
    Abstract: Described herein are variants of H. jecorina CBH2, a Cel6A enzyme. The present invention provides novel cellobiohydrolases that have altered thermostability.
    Type: Grant
    Filed: July 7, 2011
    Date of Patent: May 6, 2014
    Assignee: Danisco US Inc.
    Inventors: Wolfgang Aehle, Frits Goedegebuur, Lydia Dankmeyer, Colin Mitchinson, Paulien Neefe, Bradley Kelemen, Robert Caldwell, Pauline Teunissen
  • Variants of a family 44 xyloglucanase
    Patent number: 8709777
    Abstract: The present invention relates to variants of a parent xyloglucanase. The present invention also relates to polynucleotides encoding the variant xyloglucanases and to nucleic acid constructs, vectors, and host cells comprising the polynucleotide.
    Type: Grant
    Filed: June 4, 2009
    Date of Patent: April 29, 2014
    Assignee: Novozymes A/S
    Inventors: Werner Besenmatter, Esben Peter Friis, Keith Gibson, Frank Winther Rasmussen, Michael Skjoet
  • RECOMBINANT THERMOASCUS AURANTIACUS BETA-GLUCOSIDASE VARIANTS FOR PRODUCTION OF FERMENTABLE SUGARS FROM CELLULOSIC BIOMASS
    Publication number: 20140113352
    Abstract: The present invention provides compositions and methods for the expression of recombinant ?-glucosidase variants, as well as their use in the production of fermentable sugars from cellulosic biomass.
    Type: Application
    Filed: December 19, 2013
    Publication date: April 24, 2014
    Applicant: Codexis, Inc.
    Inventors: Dipnath Baidyaroy, Louis Clark, Lisa M. Newman
  • METHODS AND COMPOSITIONS FOR THE PROVISION OF PROTEINS DEFICIENT IN LYSOSOMAL STORAGE DISEASES
    Publication number: 20140112896
    Abstract: Nucleases and methods of using these nucleases for inserting a sequence encoding a therapeutic protein such as an enzyme into a cell, thereby providing proteins or cell therapeutics for the provision of proteins lacking or deficient in subjects with a lysosomal storage disease and treatment and/or prevention of lysosomal storage diseases.
    Type: Application
    Filed: July 11, 2013
    Publication date: April 24, 2014
    Inventor: Edward J. Rebar
  • Polypeptides having cellulolytic enhancing activity and polynucleotides encoding same
    Publication number: 20140115739
    Abstract: The present invention relates to isolated polypeptides having cellulolytic enhancing activity and isolated polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides.
    Type: Application
    Filed: October 11, 2013
    Publication date: April 24, 2014
    Inventors: Suchindra Maiyuran, Randall Kramer, Paul Harris