Acting On Beta-1, 4-glucosidic Bond (e.g., Cellulase, Etc. (3.2.1.4)) Patents (Class 435/209)
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Publication number: 20150031080Abstract: The present invention relates to variants of a parent cellobiohydrolase II. The present invention also relates to polynucleotides encoding the variants; nucleic acid constructs, vectors, and host cells comprising the polynucleotides; and methods of using the variants.Type: ApplicationFiled: October 13, 2014Publication date: January 29, 2015Inventor: Mark Wogulis
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Patent number: 8940515Abstract: The present invention relates to isolated polypeptides having xylanase activity and isolated polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides.Type: GrantFiled: November 8, 2010Date of Patent: January 27, 2015Assignee: Novozymes, Inc.Inventors: Alfredo Lopez de Leon, Michael Rey
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Publication number: 20150024471Abstract: Whole corn kernels or particles thereof are enzymatically disassembled. The method can produce a solid starch fraction, a solid pericarp fraction, and a liquid fraction. A high purity starch solids product can be provided suitable for use as a feedstock in other chemical processes.Type: ApplicationFiled: October 10, 2014Publication date: January 22, 2015Inventors: Michael R. Ladisch, Youngmi Kim, Richard L. Hendrickson
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Publication number: 20150024439Abstract: The present invention relates to isolated polypeptides having cellulolytic enhancing activity and isolated polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods for producing and using the polypeptides.Type: ApplicationFiled: September 29, 2014Publication date: January 22, 2015Inventors: Kimberly Brown, Paul Harris, Elizabeth Zaretsky, Edward Re, Elena Vlasenko, Keith McFarland, Alfredo Lopez de Leon
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Publication number: 20150024465Abstract: The present invention relates to non-catalytic carbohydrate-binding modules (CBM) belonging to a new family of CBM's. A CBM of the invention was found attached to a glycosyl hydrolase family 61 (GH61) polypeptide and was shown to have little homology with known CBM's indicating that it is the first known member of a new family of CBM's. The present invention further relates to CBM's preferably exhibiting binding affinity for cellulose; to a method of producing such CBM's; and to methods for using such CBM's in the textile, detergent and cellulose fiber processing industries, for purification of polypeptides, immobilisation of active enzymes, baking, manufacturing of biofuel, modification of plant cell walls.Type: ApplicationFiled: September 29, 2014Publication date: January 22, 2015Inventors: Kirk Matthew Schnorr, Lars Lehmann Hylling Christensen
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Patent number: 8936929Abstract: The present invention relates to novel xylose-fermenting yeast strains (for example, yeast of the genus Saccharomyces, e.g., S. cerevisiae) with an enhanced ability to ferment the xylose (and/or another pentose sugar) present in a lignocellulosic hydrolysate to a fermentation product(s) (for example, an alcohol (e.g., ethanol) or a sugar alcohol (e.g., xylitol)).Type: GrantFiled: October 3, 2013Date of Patent: January 20, 2015Assignee: Iogen Energy CorporationInventors: Jennifer Donaldson, Jan-Maarten A. Geertman, Glenn D. Munkvold, Gary Pigeau, Philippe J. Dufresne, Loreta Gudynaite-Savitch
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Publication number: 20150017690Abstract: The present invention relates to a polypeptide which has ?-glucosidase activity, and which includes an amino acid sequence represented by SEQ ID NO: 1, a polypeptide including an amino acid sequence in which one or several amino acids are deleted, substituted, or added in the amino acid sequence represented by SEQ ID NO: 1, or a polypeptide including an amino acid sequence having 91% or greater sequence identity with the amino acid sequence represented by SEQ ID NO: 1. According to the present invention, a novel ?-glucosidase enzyme derived from Acremonium cellulolyticus, a polynucleotide encoding the ?-glucosidase, an expression vector for expressing the ?-glucosidase, a transformant incorporated with that expression vector, and a method for producing a cellulose degradation product using the ?-glucosidase can be provided.Type: ApplicationFiled: July 7, 2014Publication date: January 15, 2015Inventors: Maiko TANAKA, Shigenobu MITSUZAWA, Satoru SHINKAWA, Daisuke SHIBATA, Takeshi ARA, Migiwa TAKEDA
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Publication number: 20150017691Abstract: The present invention relates to a polypeptide which has ?-glucosidase activity, and which includes an amino acid sequence represented by SEQ ID NO: 1, a polypeptide including an amino acid sequence in which one or several amino acids are deleted, substituted, or added in the amino acid sequence represented by SEQ ID NO: 1, or a polypeptide including an amino acid sequence having 92% or greater sequence identity with the amino acid sequence represented by SEQ ID NO: 1. According to the present invention, a novel ?-glucosidase enzyme derived from Acremonium cellulolyticus, a polynucleotide encoding the ?-glucosidase, an expression vector for expressing the ?-glucosidase, a transformant incorporated with that expression vector, and a method for producing a cellulose degradation product using the ?-glucosidase can be provided.Type: ApplicationFiled: July 7, 2014Publication date: January 15, 2015Inventors: Maiko TANAKA, Shigenobu MITSUZAWA, Satoru SHINKAWA, Daisuke SHIBATA, Takeshi ARA, Migiwa TAKEDA
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Publication number: 20150020235Abstract: A method for carrying out recombination at a target locus in a Rasamsonia cell, which method comprises: providing two or more nucleic acids which, when taken together, comprise: (a) sequences capable of homologous recombination with sequences flanking the target locus; (b) two or more site-specific recombination sites; (c) a sequence encoding a recombinase which recognizes the site-specific recombination sites; and (d) a sequence encoding a marker, wherein the two or more nucleic acids are capable of homologous recombination with each other so as to give rise to a single nucleic acid, and wherein at least two of the two or more nucleic acids each comprise a sequence encoding a non-functional portion of the marker; and recombining in a Rasamsonia cell the said two or more nucleic acids with each other and with the sequences flanking the target locus so that a contiguous nucleic acid sequence encoding a functional marker and the sequence encoding the recombinase are inserted at the target locus, said marker-encType: ApplicationFiled: March 12, 2013Publication date: January 15, 2015Inventors: Alrik Pieter Los, Yvonne Johannes Odilia Arendsen, Adrianus Wilhelmus Hermanus Vollebregt
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Publication number: 20150017692Abstract: The present invention relates to a polypeptide which has ?-glucosidase activity, and which includes an amino acid sequence represented by SEQ ID NO: 1, a polypeptide including an amino acid sequence in which one or several amino acids are deleted, substituted, or added in the amino acid sequence represented by SEQ ID NO: 1, or a polypeptide including an amino acid sequence having 92% or greater sequence identity with the amino acid sequence represented by SEQ ID NO: 1. According to the present invention, a novel ?-glucosidase enzyme derived from Acremonium cellulolyticus, a polynucleotide encoding the ?-glucosidase, an expression vector for expressing the ?-glucosidase, a transformant incorporated with that expression vector, and a method for producing a cellulose degradation product using the ?-glucosidase can be provided.Type: ApplicationFiled: July 7, 2014Publication date: January 15, 2015Inventors: Maiko TANAKA, Shigenobu MITSUZAWA, Satoru SHINKAWA, Daisuke SHIBATA, Takeshi ARA, Migiwa TAKEDA
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Publication number: 20150017689Abstract: The present invention relates to a polypeptide which has ?-glucosidase activity, and which includes an amino acid sequence represented by SEQ ID NO: 1, a polypeptide including an amino acid sequence in which one or several amino acids are deleted, substituted, or added in the amino acid sequence represented by SEQ ID NO: 1, or a polypeptide including an amino acid sequence having 90% or greater sequence identity with the amino acid sequence represented by SEQ ID NO: 1. According to the present invention, a novel ?-glucosidase enzyme derived from Acremonium cellulolyticus, a polynucleotide encoding the ?-glucosidase, an expression vector for expressing the ?-glucosidase, a transformant incorporated with that expression vector, and a method for producing a cellulose degradation product using the ?-glucosidase can be provided.Type: ApplicationFiled: July 3, 2014Publication date: January 15, 2015Inventors: Maiko TANAKA, Shigenobu MITSUZAWA, Satoru SHINKAWA, Daisuke SHIBATA, Takeshi ARA, Migiwa TAKEDA
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Publication number: 20150010960Abstract: An object of the present invention is to provide a soft biomass decomposition method, a production method for a target substance from soft biomass, and an enzyme or group of enzymes for decomposing soft biomass. Provided is a soft biomass decomposition method, including a step of bringing an enzyme selected from specific exocellulase, endocellulase, and processive endocellulase into contact with soft biomass such as bagasse and rice straw. Also provided is a production method for a target substance from soft biomass by incorporating the soft biomass decomposition method as a step. Further provided is an enzyme or group of enzymes for decomposing soft biomass selected from specific exocellulase, endocellulase, and processive endocellulase.Type: ApplicationFiled: January 29, 2013Publication date: January 8, 2015Applicant: NATIONAL UNIVERSITY CORPORATION MIE UNIVERSITYInventors: Yutaka Tamaru, Takeshi Katsuyama
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Publication number: 20150010981Abstract: The present invention provides various GH61 protein variants comprising various amino acid substitutions. The GH61 protein variants have an improved ability to synergize with cellulase enzymes, thereby increasing the yield of fermentable sugars obtained by saccharification of biomass. In some embodiments, sugars obtained from saccharification are fermented to produce numerous end-products, including but not limited to alcohol.Type: ApplicationFiled: September 25, 2014Publication date: January 8, 2015Inventors: Jie Yang, Xiyun Zhang, Jungjoo Yoon, Kripa K. Rao, John H. Grate, David M. Elgart, Dipnath Baidyaroy
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Publication number: 20150010961Abstract: The present invention relates to a family 5 glycoside hydrolase variant having endoglucanase activity, polynucleotides encoding the family 5 glycoside hydrolase variant, vectors, host cells comprising the polynucleotides, and methods for using the family 5 glycoside hydrolase variant.Type: ApplicationFiled: September 25, 2014Publication date: January 8, 2015Inventors: John Poland, Justin T. Stege
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Publication number: 20150004656Abstract: The present invention relates to isolated polypeptides having cellulolytic enhancing activity and isolated polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides.Type: ApplicationFiled: September 15, 2014Publication date: January 1, 2015Inventors: Lan Tang, Ye Liu, Junxin Duan, Yu Zhang, Christian Joergensen, Randall Kramer
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Publication number: 20150004655Abstract: The present invention relates to cellobiohydrolase variants. The present invention also relates to polynucleotides encoding the variants; nucleic acid constructs, vectors, and host cells comprising the polynucleotides; and methods of producing and using the variants.Type: ApplicationFiled: December 20, 2012Publication date: January 1, 2015Inventor: Mark Wogulis
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Publication number: 20150004670Abstract: Methods of continuous production of a product of interest using a filamentous fungus are provided. The fungus is auxotrophic for a nutrient that is required for fungal growth, but not for fungal synthesis of the product of interest. Under growth limiting culture conditions, in which the nutrient is absent from the culture medium, the amount of fungus remains constant or nearly constant and nutrients are not used up for growth but instead are available for synthetic pathways which produce the product of interest.Type: ApplicationFiled: June 27, 2014Publication date: January 1, 2015Inventors: MICHAEL MUELLER, MARK WILKINS, ROLF A. PRADE
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Publication number: 20140377811Abstract: The present invention concerns micro-organisms which present cellulases on their surface. Corresponding micro-organisms were produced with the aid of corresponding plasmids which encode a section comprising a signal peptide, a heterologous cellulase, an optional protease recognition site, a transmembrane linker and a transporter domain of an autotransporter or a variant thereof. Such micro-organisms were advantageously used in the conversion of cellulose into cellobiose and/or glucose. It was also possible to recover the micro-organisms from the reaction mixture following conversion from simple substrates. Also, a combination of various micro-organisms, which were populated with exocellulases, endocellulases and beta-glucosidases, were used to produce glucose from cellulose or wood.Type: ApplicationFiled: March 11, 2014Publication date: December 25, 2014Applicant: ZYRUS Beteiligungsgesellschaft mbH & Co. Patente I KGInventors: Michael Jurgens, Andreas Rohardt, Ruth Maas, Tatjana Brossette
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Patent number: 8916366Abstract: The invention relates to a multi-cellulase enzyme composition for the enzymatic hydrolysis of cellulosic biomass said composition comprising a cellobiohydrolase (CBH) enzyme, an endoglucanase (EG) enzyme and a ?-glucosidase (BG) enzyme.Type: GrantFiled: November 18, 2010Date of Patent: December 23, 2014Assignee: Codexis, Inc.Inventors: Kripa Rao, Ish Dhawan, Sally Postlethwaite, Jie Yang
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Patent number: 8916363Abstract: This invention provides novel enzyme compositions using newly identified and isolated C. lucknowense enzymes, including CBH Ib CBH IIb, EG II, EG VI, ?-glucosidase, and xylanase II in conjunction with previously identified enzymes CBH Ia, CBH IIa (previously described as Endo 43), and EG V. These enzyme compositions demonstrate an extremely high ability to convert lignocellulosic biomass (e.g., Avicel, cotton, Douglas fir wood pretreated by organosolv) to glucose. CBH Ia and IIb, which both have a cellulose-binding module (CBM) displayed a pronounced synergism with three major endoglucanases (EG II, EG V, EG VI) from the same fungus in hydrolysis of cotton as well as a strong synergy with each other. The enzyme compositions are effective in hydrolysis of the lignocellulosic biomass.Type: GrantFiled: October 20, 2010Date of Patent: December 23, 2014Assignee: Dyadic International (USA), Inc.Inventors: Alexander V. Gusakov, Tatyana N. Salanovich, Alexey I. Antonov, Boris B. Ustinov, Oleg N. Okunev, Richard P. Burlingame, Mark A. Emalfarb, Marco A. Baez, Arkady P. Sinitsyn
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Publication number: 20140370552Abstract: The present invention provides endoglucanase 1b (EG1b) variants suitable for use in saccharification reactions. The present application further provides genetically modified fungal organisms that produce EG1b variants, as well as enzyme mixtures exhibiting enhanced hydrolysis of cellulosic material to fermentable sugars, enzyme mixtures produced by the genetically modified fungal organisms, and methods for producing fermentable sugars from cellulose using such enzyme mixtures.Type: ApplicationFiled: December 18, 2012Publication date: December 18, 2014Inventors: Ee Lui Ang, Ellen D. Eberhard, Xiyun Zhang, Wei Zhang, Jing Tian, Derek J. Smith, Vesna Mitchell
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Publication number: 20140363846Abstract: The present invention provides a fermentation process for producing a cellulase enzyme mixture comprising the steps of (a) providing a fungal cell of the genus Myceliophthora or a taxonomically equivalent genus; (b) culturing the fungal cell in a submerged liquid batch culture in which the carbon source comprises about 0% of cellulase-inducing carbon source; (c) culturing the fungal cell from the batch culture from step (b) in a submerged liquid fed-batch and/or continuous culture; and (d) providing the culture of step (c) with a feed solution containing a about 100 wt % non-inducing carbon source (such as glucose, dextrose, sucrose, molasses, fructose, glycerol, xylose, or a combination thereof), wherein the feed solution is provided at a rate that maintains the concentration of non-inducing carbon source in the culture below that which would otherwise repress production of the cellulase enzyme mixture, so as to produce a culture filtrate containing at least 10 g protein/L.Type: ApplicationFiled: August 24, 2012Publication date: December 11, 2014Applicant: IOGEN ENERGY CORPORATIONInventors: Jason B. Edwards, Glenn Munkvold
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Patent number: 8906689Abstract: The present invention relates to variant endoglucanases having improved thermoactivity, improved thermostability, and improved viscosity reduction activity over wild-type M. thermophila endoglucanase.Type: GrantFiled: December 20, 2011Date of Patent: December 9, 2014Assignee: Codexis, Inc.Inventors: Xiyun Zhang, Sachin Patil, Jie Yang, Ish Kumar Dhawan
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Publication number: 20140349373Abstract: The present invention concerns a process for the production of an enzymatic cocktail by submerged culture with a cellulolytic microorganism, comprising two phases: a phase a) for growth of said microorganism in the presence of at least one carbonaceous growth substrate in a closed reactor, said growth phase being carried out with a concentration of carbonaceous growth substrate in the range 10 to 90 g/L; a phase b) for the production of the enzymatic cocktail, in which at least one carbonaceous inducer substrate is supplied, said carbonaceous inducer substrate being at least one solid residue obtained from the step for enzymatic hydrolysis of lignocellulosic materials which have undergone a pre-treatment step, said production phase being carried out with a concentration of carbonaceous production substrate in the range 150 to 400 g/L.Type: ApplicationFiled: November 27, 2012Publication date: November 27, 2014Applicant: IFP ENERGIES NOUVELLESInventors: Fadhel Ben Chaabane, Sylvain Louret
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Publication number: 20140342410Abstract: The present invention relates to isolated polypeptides having cellobiohydrolase activity and polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides.Type: ApplicationFiled: May 14, 2014Publication date: November 20, 2014Applicant: Novozymes Inc.Inventor: Nikolaj Spodsberg
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Patent number: 8889119Abstract: This disclosure relates to enhancing growth and/or activity of lactobacilli using a prebiotic formulation which includes iso-malto oligosaccharides and ?-galactosidase; and to enhancing growth and/or activity of bifidobacteria using a prebiotic formulation which includes iso-malto oligosaccharides and ?-glucanase. Other combinations of fibers and enzymes are described below which also stimulate growth and activity of lactobacilli or bifidobacteria. These combinations of enzymes and prebiotics can be taken separately or added to foods, including desserts.Type: GrantFiled: September 7, 2013Date of Patent: November 18, 2014Assignee: Master Supplements, Inc.Inventors: Randolph S. Porubcan, Sonja L. Yonak
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Publication number: 20140335573Abstract: Fusion proteins including a type-II cohesin module that are capable of integrating into native and designer cellulosomes. ?-glucosidases modified to include a type-II cohesin module and polynucleotides encoding same. Multi-enzyme complexes including the fusion proteins, and methods for biomass degradation utilizing same.Type: ApplicationFiled: July 30, 2014Publication date: November 13, 2014Inventors: Edward A. Bayer, Gilad Gefen, Michael Anbar
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Publication number: 20140335570Abstract: The present invention relates to isolated polypeptides having xylanase activity and polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides.Type: ApplicationFiled: May 8, 2014Publication date: November 13, 2014Applicant: Novozymes Inc.Inventor: Nikolaj Spodsberg
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Publication number: 20140329293Abstract: The present invention relates to isolated polypeptides having cellobiohydrolase activity and polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides.Type: ApplicationFiled: February 12, 2013Publication date: November 6, 2014Applicants: NOVOZYMES INC., NOVOZYMES A/SInventor: Nikolaj Spodsberg
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Publication number: 20140329283Abstract: The present invention relates to new enzymes with improved properties and to compositions comprising these enzymes suitable for use in the production of a food, feed, or malt beverage product, such as in a brewing process.Type: ApplicationFiled: September 14, 2012Publication date: November 6, 2014Applicant: DUPONT NUTRITION BIOSCIENCES APSInventors: Jens Frisbæk Sørensen, Lone Brønd Miller
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Publication number: 20140329284Abstract: The present invention relates to chimeric polypeptides having beta-glucosidase activity.Type: ApplicationFiled: September 28, 2012Publication date: November 6, 2014Inventors: Mark Wogulis, David Osborn, Tia Heu
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Publication number: 20140329292Abstract: This invention relates to novel enzymes and novel methods for producing the same. More specifically this invention relates to a variety of fungal enzymes. Nucleic acid molecules encoding such enzymes, compositions, recombinant and genetically modified host cells, and methods of use are described. The invention also relates to a method to convert lignocellulosic biomass to fermentable sugars with enzymes that degrade the lignocellulosic material and novel combinations of enzymes, including those that provide a synergistic release of sugars from plant biomass. The invention also relates to a method to release cellular content by degradation of cell walls. The invention also relates to methods to use the novel enzymes and compositions of such enzymes in a variety of other processes, including washing of clothing, detergent processes, biorefining, deinking and biobleaching of paper and pulp, and treatment of waste streams.Type: ApplicationFiled: September 6, 2013Publication date: November 6, 2014Applicant: DYADIC INTERNATIONAL, INC.Inventors: Mark A. EMARLFARB, Alexander Vasilievich Gusakov, Peter J. Punt, Jan Cornelis Verdoes, Arkady Panteleimonovich Sinitsyn, Elena Vlasenko, Sandra Wihelmina Agnes Hinz, Mark Gosink, Zhijie Jiang
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Publication number: 20140331364Abstract: Provided are isolated polypeptides having beta-glucosidase activity and polynucleotides encoding the polypeptides. Also provided are nucleic acid constructs, vectors and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides.Type: ApplicationFiled: December 19, 2012Publication date: November 6, 2014Inventors: Ye Liu, Junxin Duan, Yu Zhang, Lan Tang
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Publication number: 20140322765Abstract: Nucleic acid sequences encoding chimeric polypeptides that exhibit enhanced cellulase activities are disclosed herein. These nucleic acids may be expressed in hosts such as fungi, which in turn may be cultured to produce chimeric polypeptides. Also disclosed are chimeric polypeptides and their use in the degradation of cellulosic materials.Type: ApplicationFiled: December 17, 2012Publication date: October 30, 2014Inventors: William S. Adney, Gregg T. Beckham, Eric Jarvis, Michael E. Himmel, Stephen R. Decker, Jeffrey G. Linger, Kara Podkaminer, John O. Baker, Larry Taylor, II, Qi Xu, Arjun Singh
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Publication number: 20140322753Abstract: The present invention relates to isolated polypeptides having endoglucanase activity and isolated polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods for producing and using the polypeptides.Type: ApplicationFiled: June 30, 2014Publication date: October 30, 2014Inventors: Paul Harris, Elena Vlasenko, Marcus Sakari Kauppinnen, Elizabeth Zaretsky, Sarah Teter, Kimberly Brown
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Publication number: 20140322755Abstract: The present disclosure provides a sophorolipid composition that can be used for inducing protein expression in a fermentation host. The sophorolipid composition described herein can be prepared from a natural sophorolipid mixture. Acid treatment of the natural sophorolipid mixture results in a mixture of monoacetylated, deacetylated, and/or diacetylated sophorolipids. The chemically modified sophorolipid composition, or isolated components of the chemically modified sophorolipid composition, can be used as inducers for protein production in filamentous fungi.Type: ApplicationFiled: June 25, 2012Publication date: October 30, 2014Inventor: Tom Tao Huang
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Publication number: 20140322796Abstract: The invention relates to recombinant expression of variant forms of C1 CBH1a and homologs thereof, having improved thermostability, low-pH tolerance, specific activity and other desirable properties. Also provided are methods for producing ethanol and other valuable organic compounds by combining cellobiohydrolase variants with cellulosic materials.Type: ApplicationFiled: May 9, 2014Publication date: October 30, 2014Applicant: Codexis, Inc.Inventors: Vesna Mitchell, Grzegorz Wojciechowski, Oscar Alvizo
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Publication number: 20140322762Abstract: The present invention relates to the production of sugar hydrolysates from cellulosic material. The method may be used e.g. for producing fermentable sugars for the production of bioethanol from lignocellulosic material. Cellulolytic enzymes and their production by recombinant technology is described, as well as uses of the enzymes and enzyme preparations.Type: ApplicationFiled: October 3, 2013Publication date: October 30, 2014Applicant: ROAL OYInventors: Jari VEHMAANPERÄ, Marika ALAPURANEN, Terhi PURANEN, Matti SIIKA-AHO, Jarno KALLIO, Satu HOOMAN, Sanni VOUTILAINEN, Teemu HALONEN, Liisa VIIKARI
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Publication number: 20140323356Abstract: A thermostable glycosidase enzymes derived from various Thermococcus, Staphylothermus and Pyrococcus organisms is disclosed. The enzymes are produced from native or recombinant host cells and can be utilized in the food processing industry, pharmaceutical industry and in the textile industry, detergent industry and in the baking industry.Type: ApplicationFiled: May 5, 2014Publication date: October 30, 2014Applicant: BP Corporation North America Inc.Inventors: Edward J. Bylina, Ronald V. Swanson, Eric J. Mathur, David E. Lam
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Publication number: 20140325711Abstract: The present invention relates to isolated polypeptides having lysozyme activity and polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides.Type: ApplicationFiled: November 23, 2012Publication date: October 30, 2014Inventors: Kirk Matthew Schnorr, Jens Erik Nielsen, Mikkel Klausen
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Publication number: 20140322764Abstract: What is aimed at is provision of an inexpensive and efficient saccharification method for lignocellulose using a thermostable xylanase and provision of a mutant xylanase that has a substitute amino acid residue, and that exhibits stable activity even under severe conditions in which enzymes easily inactivate, and that provides an initial rate of reaction not significantly reduced as compared to a wild-type xylanase corresponding to the mutant xylanase. Provided is a method of producing a saccharified product of lignocellulose, including contacting a lignocellulosic raw material with a thermostable xylanase, and a mutant xylanase that provides an initial rate of reaction that is at least 70% of that provided by a wild-type xylanase corresponding thereto, that has a xylanase activity after heat treatment at 50° C. for 24 hours that is at least 50% of its xylanase activity before the heat treatment, and that has a substitute amino acid residue.Type: ApplicationFiled: November 22, 2012Publication date: October 30, 2014Inventors: Hisaaki Yanai, Hiroki Tamai, Masami Osabe, Fumikazu Yokoyama, Kaoru Okakura, Atsushi Inoue
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Patent number: 8871479Abstract: The invention relates to applications of the cellulase Cel5H of Saccharophagus degradans and its homologues, functional fragments and/or variants and engineered forms thereof, in the context of recombinant, more particularly solventogenic microorganisms, more particularly C. acetobutylicum. The invention also characterizes a novel domain of the Cel5H cellulase with a putative cellulose-binding module function, and its uses in chimeric proteins for depolymerization of cellulose containing substrates.Type: GrantFiled: November 26, 2009Date of Patent: October 28, 2014Assignees: Total Marketing Services, Le Centre National de Recherche Scientifiques (CNRS), L'Institut National des Sciences Appliquees (INSA), Universite d'Aix-MarseilleInventors: Henri-Pierre Fierobe, Angélique Chanal-Vial, Anne-Laure Molinier, Chantal Tardif, Luc Dedieu
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Patent number: 8871486Abstract: The present invention relates to methods for producing a secreted polypeptide having biological activity, comprising: (a) transforming a fungal host cell with a fusion protein construct encoding a fusion protein, which comprises: (i) a first polynucleotide encoding a signal peptide; (ii) a second polynucleotide encoding at least a catalytic domain of an endoglucanase or a portion thereof; and (iii) a third polynucleotide encoding at least a catalytic domain of a polypeptide having biological activity; wherein the signal peptide and at least the catalytic domain of the endoglucanase increases secretion of the polypeptide having biological activity compared to the absence of at least the catalytic domain of the endoglucanase; (b) cultivating the transformed fungal host cell under conditions suitable for production of the fusion protein; and (c) recovering the fusion protein, a component thereof, or a combination thereof, having biological activity, from the cultivation medium.Type: GrantFiled: May 16, 2014Date of Patent: October 28, 2014Assignee: Novozymes, Inc.Inventor: Sandra Merino
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Publication number: 20140317786Abstract: The present invention relates to isolated polypeptides having beta-glucosidase activity, beta-xylosidase activity, or beta-glucosidase and beta-xylosidase activity and isolated polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides.Type: ApplicationFiled: November 16, 2012Publication date: October 23, 2014Inventor: Marc Morant
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Publication number: 20140315313Abstract: Described are compositions and methods relating to variant filamentous fungi having altered growth characteristics. Such variants are well-suited for growth in submerged cultures, e.g., for the large-scale production of enzymes and other proteins for commercial applications.Type: ApplicationFiled: April 20, 2012Publication date: October 23, 2014Applicant: DANISCO US INC.Inventors: Elizabeth A. Bodie, Robert James Pratt, II
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Publication number: 20140315246Abstract: The present invention provides a novel endo-?-N-acetylglucosaminidase (Endo-Om) using a transformant produced by cloning an endo-?-N-acetylglucosaminidase (Endo-Om) gene originated from a methylotrophic yeast Ogataea minuta IFO10746 strain. The Endo-Om according to the present invention has a specific activity 13-fold higher than that of known Endo-M and a Vmax value 55-fold higher than that of the known Endo-M, and is useful for the analysis of the structures of sugar chains, including complex type sugar chains, in glycoproteins and the modification of the sugar chains.Type: ApplicationFiled: October 3, 2012Publication date: October 23, 2014Applicant: National Institute of Advanced Industrial Science and TechnologyInventors: Yasunori Chiba, Satoshi Murakami, Hisashi Narimatsu
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Publication number: 20140308713Abstract: A variant Cel5a endoglucanase has increased thermostability, increased enzymatic activity and/or increased expression in a host, relative to wild type Cel5a. The improved variant Cel5a endoglucanase may be used to hydrolyze more cellulose at a higher temperature for a more efficient and cost-effective production of biofuels as compared to wild type Cel5a.Type: ApplicationFiled: April 7, 2014Publication date: October 16, 2014Inventors: Toni M. Lee, Stephen L. Mayo, Frances H. Arnold, Devin Trudeau
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Publication number: 20140308701Abstract: The present invention relates to recombinant filamentous fungal host cells producing cellulolytic enzyme compositions and methods of producing and using the compositions.Type: ApplicationFiled: August 23, 2012Publication date: October 16, 2014Inventors: Jeffrey Shasky, Amanda Fischer, Suchindra Maiyuran
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Publication number: 20140308705Abstract: The present invention relates to isolated polypeptides having cellulolytic enhancing activity and polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides.Type: ApplicationFiled: September 20, 2012Publication date: October 16, 2014Inventors: Marc Morant, Tarana Shaghasi
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Publication number: 20140304859Abstract: Provided are isolated polypeptides having endoglucanase activity and polynucleotides encoding the polypeptides. Also provided are nucleic acid constructs, vectors and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides.Type: ApplicationFiled: December 14, 2012Publication date: October 9, 2014Applicant: Novozymes Inc.Inventors: Ye Liu, Yu Zhang, Junxin Duan, Lan Tang