Bacteria Is Bacillus Patents (Class 435/221)
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Patent number: 8569035Abstract: The present invention relates to novel subtilase variants exhibiting alterations relative to the parent subtilase in one or more properties including: Wash performance, thermal stability, storage stability or catalytic activity. The variants of the invention are suitable for use in e.g. cleaning or detergent compositions, such as laundry detergent compositions and dishwash compositions, including automatic dishwash compositions.Type: GrantFiled: April 29, 2013Date of Patent: October 29, 2013Assignee: Novozymes A/SInventors: Tina Sejersgard Fano, Claus Von Der Osten, Malene Kappen Kruger, Mads Norregaard-Madsen
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Patent number: 8557553Abstract: The present disclosure relates to a method for reducing nuclease activity in a subtilisin solution obtained from a fermentation broth including: a) adjusting the pH of the subtilisin solution to a pH in the range of from pH 8.5 to pH 10.5; b) adding a polyol; and c) adjusting the temperature of the subtilisin solution to a temperature in the range of from 50° C. to 80° C. In accordance with the present disclosure, the nuclease activity is reduced to less than 5% of the initial value and the subtilisin activity is maintained at more than 60% of the initial value.Type: GrantFiled: August 19, 2008Date of Patent: October 15, 2013Assignee: Novozymes A/SInventors: Kim Uhre Hansen, Peter Rahbek Oestergaard
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Publication number: 20130260438Abstract: The present invention provides serine protease variants produced there from. Specifically, the present invention provides serine protease variants having one or more substitutions as compared to a reference serine protease. In addition, the present invention provides compositions comprising these serine protease variants. In some embodiments, the present invention provides cleaning compositions comprising at least one of these serine protease variants.Type: ApplicationFiled: May 5, 2011Publication date: October 3, 2013Applicant: Danisco US Inc.Inventors: Viktor Yuryevich Alekseyev, Neelam A Amin, Katherine Auguslyn, Luis Gustavo Cascao-Pereira, Katherine D. Collier, David A. Estell, James T. Kellis, JR., Ayrookaran J. Poulose, Philip Frank Souter, Glenn Steven Ward, Jian Yao
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Patent number: 8530218Abstract: The present invention provides methods and compositions for the production of mature proteases in bacterial host cells. The compositions include modified polynucleotides that encode modified proteases, which have at least one mutation in the pro region; the modified serine proteases encoded by the modified polynucleotides; expression cassettes, DNA constructs, and vectors comprising the modified polynucleotides that encode the modified proteases; and the bacterial host cells transformed with the vectors of the invention. The methods include methods for enhancing the production of mature proteases in bacterial host cells e.g. Bacillus sp. host cells. The produced proteases find use in the industrial production of enzymes, suitable for use in various industries, including but not limited to the cleaning, animal feed and textile processing industry.Type: GrantFiled: April 15, 2010Date of Patent: September 10, 2013Assignee: Danisco US Inc.Inventors: Eugenio Ferrari, Carol Fioresi, Anita van Kimmenade
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Patent number: 8530219Abstract: The present invention provides a Bacillus sp. subtilisin variant. In addition, the present invention provides compositions comprising this serine protease variant. In some embodiments, the present invention provides laundry and other non-automatic (i.e., hand) dishwashing cleaning compositions comprising this serine protease variant.Type: GrantFiled: November 10, 2009Date of Patent: September 10, 2013Assignee: Danisco US Inc.Inventors: David A. Estell, Frits Goedegebuur, Ayrookaran Poulose
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Publication number: 20130225466Abstract: The present invention relates to novel subtilase variants exhibiting alterations relative to the parent subtilase in one or more properties including: Wash performance, thermal stability, storage stability or catalytic activity. The variants of the invention are suitable for use in e.g. cleaning or detergent compositions, such as laundry detergent compositions and dishwash compositions, including automatic dishwash compositions.Type: ApplicationFiled: April 29, 2013Publication date: August 29, 2013Applicant: Novozymes A/SInventor: Novozymes A/S
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Publication number: 20130196415Abstract: A microorganism belonging to the genus Bacillus, wherein the microorganism belonging to the genus Bacillus is any one of a Bacillus subtilis 104-1-3-1 strain (Accession No: NITE BP-680) and a derivative strain thereof, and wherein the microorganism belonging to the genus Bacillus produces a thrombolytic enzyme which decomposes waste.Type: ApplicationFiled: March 14, 2013Publication date: August 1, 2013Applicant: MICROBIAL CHEMISTRY RESEARCH FOUNDATIONInventor: MICROBIAL CHEMISTRY RESEARCH FOUNDATION
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Publication number: 20130171717Abstract: Novel enzyme variants including protease variants derived from the DNA sequences of naturally-occurring or recombinant non-human proteases are disclosed. The variant proteases, in general, are obtained by in vitro modification of a precursor DNA sequence encoding the naturally-occurring or recombinant protease to generate the substitution of a plurality of amino acid residues in the amino acid sequence of a precursor protease. Such variant proteases have properties which are different from those of the precursor protease, such as altered wash performance. The substituted amino acid residue correspond to positions 27, 45, 170, 181, 251 and 271 of Bacillus amyloliquefaciens subtilisin. Additional variants comprising at least one additional substitution at a position selected from 1, 14, 49, 61, 87, 100, 102, 118, 128, 204 and 258 of Bacillus amyloliquefaciens subtilisin are also described.Type: ApplicationFiled: December 21, 2012Publication date: July 4, 2013Applicant: DANISCO US INC.Inventor: Danisco US Inc.
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Publication number: 20130157341Abstract: Novel protease variants derived from the DNA sequences of naturally-occurring or recombinant non-human proteases are disclosed. The variant proteases, in general, are obtained by in vitro modification of a precursor DNA sequence encoding the naturally-occurring or recombinant protease to generate the substitution of a plurality of amino acid residues in the amino acid sequence of a precursor protease. Such variant proteases have properties which are different from those of the precursor protease, such as altered wash performance. The substituted amino acid residue equivalent to positions 7, 23, 26, 28, 29, 30, 31, 47, 66, 69, 73, 82, 85, 88, 90, 92, 93, 105, 113, 139, 148, 149, 150, 151, 178, 200, 201, 231, 233, 267 and/or 273 of Bacillus amyloliquefaciens subtilisin.Type: ApplicationFiled: November 7, 2012Publication date: June 20, 2013Applicant: DANISCO US INC.Inventor: Danisco US Inc.
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Publication number: 20130156884Abstract: A food supplement comprises at least one protease enzyme selected from (i) CAS #9001-92-7, IUB 3.4.23.18 protease from Aspergillus; (ii) CAS #9001-92-7, IUB 3.4.21.7 protease from Bacillus; (iii) CAS #9001-61-0, IUB 3.4.11.1 protease from Aspergillus; (iv) CAS #9074-07-1, IUB 3.4.21.63 protease from Aspergillus; (v) CAS #9073-78-3, IUB 3.4.24.27 protease from Aspergillus; (vi) CAS #9025-49-4, IUB 3.4.23.18 protease from Aspergillus; and (vii) combinations thereof. The food supplement may further comprise a protein, a stabilizer, a matrix modifier, a carrier, a preservative, a bulking agent, a dessicant, an emulsifier, an enzyme coating, or combinations thereof. Disclosed is a method of increasing protein absorption in the gastrointestinal system of a human being comprising the step of ingesting the food supplement as identified above.Type: ApplicationFiled: December 19, 2011Publication date: June 20, 2013Applicant: TRIARCO INDUSTRIESInventor: Mark L. Anderson
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Publication number: 20130122548Abstract: The present invention relates to an isolated mutant eubacterium comprising at least one mutation resulting in a substitution of at least one amino acid in the beta-subunit of the RNA-polymerase encoded for by the rpoB-gene providing an altered production of a product of interest when said production of a product of interest is compared to the production of the same product in an isogenic wild type strain grown at identical conditions, wherein the substitution of at least one amino acid occurs at any of positions 469, 478, 482, 485, or 487 of SEQ ID NO:2, or at the equivalent positions in any eubacterial RNA-polymererase beta-subunit family member. Another aspect of the invention relates to a process for producing at least one product of interest in a mutant eubacterium and to a use of the mutant eubacterium according to the invention for producing at least one product of interest.Type: ApplicationFiled: January 22, 2013Publication date: May 16, 2013Applicant: NOVOZYMES A/SInventor: Novozymes A/S
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Patent number: 8440180Abstract: There is provided an anti-cancer composition comprising an aquatic microbial extract, and there is more particularly provided an anti-cancer composition comprising a Bacillus sp. strain extract.Type: GrantFiled: June 26, 2008Date of Patent: May 14, 2013Assignee: Ajou University Industry-Academic Cooperation FoundationInventors: Chul-Ho Kim, Ki-Woong Cho
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Publication number: 20130078705Abstract: A protease for treating nail abnormalities is a type of protease. Since nails are mostly composed of keratin protein, the optimum protease for decomposing the main component (keratin) of the nail is keratinase or serine protease which is functionally similar to keratinase. The serine protease include trypsin, chymotrypsin, elastase, subtilisin, etc, these enzymes can be used to treat pathologically or mechanically damaged nail tissue to thin or remove keratin which is the key component of the nail, thus facilitating drug delivery in later treatment.Type: ApplicationFiled: September 22, 2011Publication date: March 28, 2013Inventor: Hsuehkuan LU
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Patent number: 8394373Abstract: The present invention relates to a disintegrin and metalloproteinase containing thrombospondin 1-like domains (ADAMTS) and in particular to a novel ADAMTS13 protease and to nucleic acids encoding ADAMTS13 proteases. The present invention encompasses both native and recombinant wild-type forms of ADAMTS13, as well as mutant and variant forms including fragments, some of which posses altered characteristics relative to the wild-type ADAMTS13. The present invention also relates to methods of using ADAMTS13, including for treatment of TTP. The present invention also relates to methods for screening for the presence of TTP. The present invention further relates to methods for developing anticoagulant drugs based upon ADAMTS13.Type: GrantFiled: April 13, 2009Date of Patent: March 12, 2013Assignee: The Regents of the University of MichiganInventors: David Ginsburg, Gallia Levy, Han-Mou Tsai
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Patent number: 8389262Abstract: The present invention relates to subtilase subtilases with an altered immunogenicity, particularly subtilases with a reduced allergenicity. Furthermore, the invention relates to expression of said subtilase variants and subtilases and to their use, such as in detergents and oral care products.Type: GrantFiled: September 23, 2010Date of Patent: March 5, 2013Assignee: Novozymes A/SInventors: Stefan Minning, Henriette Draborg, Erwing Ludo Roggen, Nanna Kristensen Soni, Ninna Willestofte Berg, Stina Thulesen Lyngstrand
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Publication number: 20130045524Abstract: The present invention relates to novel JP170 like subtilases from wild-type bacteria, hybrids thereof and to methods of construction and production of these proteases. Further, the present invention relates to use of the claimed subtilases in detergents, such as a laundry or an automatic dishwashing detergent.Type: ApplicationFiled: October 24, 2012Publication date: February 21, 2013Applicant: NOVOZYMES A/SInventors: Preben Nielsen, Poul Erik Pedersen, Helle Outtrup
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Publication number: 20130029402Abstract: The present invention provides host cells that have been genetically manipulated to have an enhanced capacity to produce proteins of interest. In particular, the invention relates to modified Bacillus sp. Host cells that have at least one inactivated phr gene. The enhanced production of proteins of interest by the modified Bacillus sp. Host cells is further increased in modified Bacillus sp. Host cells that overexpress YmaH.Type: ApplicationFiled: September 19, 2012Publication date: January 31, 2013Applicant: DANISCO US INC.Inventor: Danisco US Inc.
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Chemically modified mutant serine hydrolases show improved catalytic activity and chiral selectivity
Patent number: 8357524Abstract: This invention provides novel chemically modified mutant serine hydrolases that catalyze a transamidation and/or a transpeptidation and/or a transesterification reaction. The modified serine hydrolases have one or more amino acid residues in a subsite replaced with a cysteine, wherein the cysteine is modified by replacing the thiol hydrogen in the cysteine with a substituent group providing a thiol side chain comprising a moiety selected from the group consisting of a polar aromatic substituent, an alkyl amino group with a positive charge, and a glycoside. In particularly preferred embodiments, the substitutents include an oxazolidinone, a C1 to C15 alkyl amino group with a positive charge, or a glycoside.Type: GrantFiled: July 30, 2012Date of Patent: January 22, 2013Assignee: Danisco US Inc.Inventors: John Bryan Jones, Michael Dickman, Richard C. Lloyd -
Patent number: 8343735Abstract: The present invention provides methods of altering the production of desired polypeptides in a host cell. In particular, the present invention provides polynucleotides encoding truncated SecG proteins capable of facilitating the secretion of desired proteases by a bacterial host cell, such as Bacillus species, as well as expression vectors and a host cell containing the polynucleotides.Type: GrantFiled: May 12, 2008Date of Patent: January 1, 2013Assignee: Danisco US Inc.Inventors: Anita Van Kimmenade, Carol Fioresi, Caroline Peres, Eugenio Ferrari
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Patent number: 8318470Abstract: The present invention relates to novel JP170 like subtilases from wild-type bacteria, hybrids thereof and to methods of construction and production of these proteases. Further, the present invention relates to use of the claimed subtilases in detergents, such as a laundry or an automatic dishwashing detergent.Type: GrantFiled: January 5, 2012Date of Patent: November 27, 2012Assignee: Novozymes A/SInventors: Preben Nielsen, Poul Erik Pedersen, Helle Outtrup
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Patent number: 8241885Abstract: The present invention is directed to the identification of a protease prodomain that is capable of binding a corresponding protease with high affinity. The protease prodomain of the present invention is fused to a second protein to form a protease prodomain fusion protein. The presence of a protease prodomain protein in a fusion protein allows for easy and selective purification of the second protein by incubation with the corresponding protease.Type: GrantFiled: September 22, 2010Date of Patent: August 14, 2012Assignee: University of Maryland, College ParkInventor: Philip N. Bryan
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Patent number: 8232087Abstract: The present invention relates to novel JP170 like subtilases from wild-type bacteria, hybrids thereof and to methods of construction and production of these proteases. Further, the present invention relates to use of the claimed subtilases in detergents, such as a laundry or an automatic dishwashing detergent.Type: GrantFiled: February 9, 2011Date of Patent: July 31, 2012Assignee: Novozymes A/SInventors: Preben Nielsen, Poul Erik Pedersen, Helle Outtrup
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Publication number: 20120172280Abstract: The present invention relates to protease variants. The present invention also relates to polynucleotides encoding the variant protease variants and to nucleic acid constructs, vectors, and host cells comprising the polynucleotides, and methods of using the variant enzymes, such as, in laundry and detergent compositions.Type: ApplicationFiled: September 24, 2010Publication date: July 5, 2012Applicant: NOVOZYMES A/SInventors: Jurgen Carsten Franz Knotzel, Maria Norman Hockauf, Lars Beier, Astrid Benie
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Publication number: 20120142076Abstract: The present invention provides host cells that have been genetically manipulated to have an enhanced capacity to produce proteins of interest. In particular, the invention relates to modified Bacillus sp. Host cells that have at least one inactivated phr gene. The enhanced production of proteins of interest by the modified Bacillus sp. Host cells is further increased in modified Bacillus sp. Host cells that overexpress YmaH.Type: ApplicationFiled: June 2, 2010Publication date: June 7, 2012Applicant: Danisco US Inc.Inventors: Cristina Bongiorni, Eugenio Ferrari
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Publication number: 20120122183Abstract: A method of flocculating a bacterial cell, producing a protein of interest, from a fermentation broth, comprising a) diluting the fermentation broth up to 1000% (w/w) with water; b) adding a divalent salt to a concentration in the fermentation broth of more than 10 milli moles per liter diluted fermentation broth; c) adjusting the phosphate concentration to a concentration in the fermentation broth of more than 10 milli moles per liter diluted fermentation broth; d) adjusting the pH of the diluted fermentation broth to a pH within the range of 6.1-10.5; and e) removing the bacterial cells, whereby a protein solution with a turbidity of less than 100 NTU is obtained.Type: ApplicationFiled: June 30, 2010Publication date: May 17, 2012Applicant: NOVOZYMES A/SInventors: Jakob Rauhe Pedersen, Peter Frode Pind
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Publication number: 20120122184Abstract: A new strain Bacillus sp. P203 (Bacillus plakortiensis) is disclosed. Isolated mature functional polypeptide which is obtainable from the bacterium strain Bacillus sp. P203 deposited under accession number DSM 17419 are disclosed.Type: ApplicationFiled: January 26, 2012Publication date: May 17, 2012Applicant: NOVOZYMES A/SInventors: Preben Nielsen, Reinhard Wilting, Martin Borchert
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Publication number: 20120107907Abstract: The present invention provides methods and compositions comprising at least one neutral metalloprotease enzyme that has improved storage stability. In some embodiments, the neutral metalloprotease finds use in cleaning and other applications. In some particularly preferred embodiments, the present invention provides methods and compositions comprising neutral metalloprotease(s) obtained from Bacillus sp. In some more particularly preferred embodiments, the neutral metalloprotease is obtained from B. amyloliquefaciens. In still further preferred embodiments, the neutral metalloprotease is a variant of the B. amyloliquefaciens neutral metalloprotease. In yet additional embodiments, the neutral metalloprotease is a homolog of the B. amyloliquefaciens neutral metalloprotease. The present invention finds particular use in applications including, but not limited to cleaning, bleaching and disinfecting.Type: ApplicationFiled: January 6, 2012Publication date: May 3, 2012Inventors: Andrew Shaw, Louise Wallace, David A. Estell, Ronald W. J. Hommes, Sang-Kyu Lee, Hiroshi Oh, Eugene S. Sadlowski
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Chemically modified mutant serine hydrolases show improved catalytic activity and chiral selectivity
Patent number: 8148128Abstract: This invention provides novel chemically modified mutant serine hydrolases that catalyze a transamidation and/or a transpeptidation and/or a transesterification reaction. The modified serine hydrolases have one or more amino acid residues in a subsite replaced with a cysteine, wherein the cysteine is modified by replacing the thiol hydrogen in the cysteine with a substituent group providing a thiol side chain comprising a moiety selected from the group consisting of a polar aromatic substituent, an alkyl amino group with a positive charge, and a glycoside. In particularly preferred embodiments, the substitutents include an oxazolidinone, a C1 to C15 alkyl amino group with a positive charge, or a glycoside.Type: GrantFiled: September 19, 2008Date of Patent: April 3, 2012Assignee: The Governing Council of the University of TorontoInventors: John Bryan Jones, Michael Dickman, Richard C. Lloyd -
Publication number: 20120052553Abstract: Novel protease variants derived from the DNA sequences of naturally-occurring or recombinant non-human proteases are disclosed. The variant proteases, in general, are obtained by in vitro modification of a precursor DNA sequence encoding the naturally-occurring or recombinant protease to generate the substitution of a plurality of amino acid residues in the amino acid sequence of a precursor protease. Such variant proteases have properties which are different from those of the precursor protease, such as altered wash performance. The substituted amino acid residue equivalent to positions 7, 23, 26, 28, 29, 30, 31, 47, 66, 69, 73, 82, 85, 88, 90, 92, 93, 105, 113, 139, 148, 149, 150, 151, 178, 200, 201, 231, 233, 267 and/or 273 of Bacillus amyloliquefaciens subtilisin.Type: ApplicationFiled: October 13, 2010Publication date: March 1, 2012Applicant: Danisco US Inc.Inventor: Ayrookaran J. Poulose
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Patent number: 8119386Abstract: The present invention relates to isolated proteases of the RP-II type and variants of RP-II proteases exhibiting improved properties in comparison to the parent RP-II protease, DNA constructs and vectors coding for the expression of said proteases and variants, host cells capable of expressing the proteases and variants from the DNA constructs, as well as a method of producing them by cultivating said host cells. The proteases may advantageously be used as constituents in detergent compositions and additives, optionally in combination with other enzymes such as proteases, lipases, cellulases, amylases, peroxidases or oxidases.Type: GrantFiled: January 26, 2011Date of Patent: February 21, 2012Assignee: Novozymes AlsInventors: Mads Norregaard-Madsen, Peter Rahbek Ostergaard, Claus Bo Voge Christensen, Soren Flensted Lassen
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Patent number: 8114656Abstract: The present invention provides methods and compositions comprising at least one neutral metalloprotease enzyme that has improved storage stability. In some embodiments, the neutral metalloprotease finds use in cleaning and other applications. In some particularly preferred embodiments, the present invention provides methods and compositions comprising neutral metalloprotease(s) obtained from Bacillus sp. In some more particularly preferred embodiments, the neutral metalloprotease is obtained from B. amyloliquefaciens. In still further preferred embodiments, the neutral metalloprotease is a variant of the B. amyloliquefaciens neutral metalloprotease. In yet additional embodiments, the neutral metalloprotease is a homolog of the B. amyloliquefaciens neutral metalloprotease. The present invention finds particular use in applications including, but not limited to cleaning, bleaching and disinfecting.Type: GrantFiled: May 28, 2009Date of Patent: February 14, 2012Assignee: Danisco US Inc.Inventors: Andrew Shaw, Louise Wallace, David A. Estell, Ronaldus W. J. Hommes, Sang-Kyu Lee, Hiroshi Oh, Eugene S. Sadlowski
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Patent number: 8101563Abstract: The present invention relates to the identification of novel metallo-proteases (MP) in Gram-positive microorganisms. The present invention provides the nucleic acid and amino acid sequences for Bacillus MP. The present invention also provides host cells having a mutation or deletion of part or all of the gene encoding MP. The present invention also provides host cells further comprising nucleic acid encoding desired heterologous proteins such as enzymes. The present invention also provides cleaning compositions comprising an MP of the present invention.Type: GrantFiled: December 8, 2004Date of Patent: January 24, 2012Assignee: Danisco US Inc.Inventor: David A. Estell
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Publication number: 20120009651Abstract: The present invention provides methods and compositions comprising at least one thermolysin-like neutral protease enzyme with improved storage stability and/or catalytic activity. In some embodiments, the thermolysin finds use in cleaning and other applications comprising detergent. In some particularly preferred embodiments, the present invention provides methods and compositions comprising thermolysin formulated and/or engineered to resist detergent-induced inactivation.Type: ApplicationFiled: October 28, 2008Publication date: January 12, 2012Applicant: DANISCO US INC.Inventors: David A. Estell, Ronaldus W.J. Hommes, Amy D. Liu, Andrew Shaw
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Publication number: 20120003718Abstract: The present invention provides engineered protease variants. In particular, the protease variants comprise combinable mutations at selected surface positions that affect the charge and/or hydrophobicity of the enzyme to enhance at least one desired property of the resulting variant enzyme in a chosen application. Compositions comprising the protease variants, and methods for using the same are also provided.Type: ApplicationFiled: November 10, 2009Publication date: January 5, 2012Applicant: Danisco US Inc.Inventors: Luis G. Cascao-Pereira, David A. Estell, James T. Kellis, JR.
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Patent number: 8084241Abstract: The present invention relates to methods for producing variants of a parent TY145 subtilase and of a parent BPN? subtilase and to TY145 and BPN? variants having altered properties as compared to the parent TY145/BPN? subtilase.Type: GrantFiled: September 10, 2010Date of Patent: December 27, 2011Assignee: Novozymes A/SInventors: Allan Svendsen, Henriette Draborg
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Publication number: 20110281328Abstract: The present invention provides a Bacillus sp. subtilisin variant. In addition, the present invention provides compositions comprising this serine protease variant. In some embodiments, the present invention provides laundry and other non-automatic (i.e., hand) dishwashing cleaning compositions comprising this serine protease variant.Type: ApplicationFiled: November 10, 2009Publication date: November 17, 2011Applicant: Danisco US Inc.Inventors: David A. Estell, Frits Goedegebuur, Ayrookaran Joseph Poulose
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Publication number: 20110262999Abstract: The present invention provides protease variants, compositions comprising protease variants, and methods of using such protease variants and compositions.Type: ApplicationFiled: December 9, 2010Publication date: October 27, 2011Applicant: Danisco US Inc.Inventors: Joshua R. Basler, Luis G. Cascão-Pereira, David A. Estell, James T. Kellis, JR., Alexander Pisarchik, Ayrookaran J. Poulose, Daniel Torres Pazmino
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Publication number: 20110256609Abstract: The present invention provides variant subtilisins and compositions comprising at least one variant subtilisin set forth herein, as well as methods for using these variants and compositions. In some embodiments, the present invention provides variant subtilisins suitable for laundry cleaning applications.Type: ApplicationFiled: June 3, 2009Publication date: October 20, 2011Inventors: Joshua R. Basler, Luis G. Cascao-Pereira, David A. Estell, James T. Kellis, JR.
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Publication number: 20110251073Abstract: The present invention provides methods for protein engineering. Specifically, the invention provides methods utilizing site evaluation libraries to design libraries that optimize two or more properties of a protein. The present invention also provides variant subtilisins suitable for various uses.Type: ApplicationFiled: June 3, 2009Publication date: October 13, 2011Inventors: Luis G. Cascao-Pereira, David A. Estell, Frits Goedegebuur, James T. Kellis, JR.
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Publication number: 20110230386Abstract: The present invention relates to enzymes produced by mutating the genes for a number of subtilases and expressing the mutated genes in suitable hosts are presented. The enzymes exhibit improved stability and/or improved wash performance in any detergent in comparison to their wild type parent enzymes. The enzymes are well-suited for use in any detergent and for some in especially liquid or solid shaped detergent compositions.Type: ApplicationFiled: May 25, 2011Publication date: September 22, 2011Applicants: Novozymes A/S, Unilever PLCInventors: Laurens Nicolaas Sierkstra, Jan Klugkist, Peter Markvardsen, Claus Von der Osten, Peter Bauditz
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Patent number: 8008057Abstract: The present invention relates to methods for producing variants of a parent JP170 subtilase and of a parent BPN? subtilase and to JP170 and BPN? variants having altered properties as compared to the parent JP170/BPN? subtilase.Type: GrantFiled: March 10, 2009Date of Patent: August 30, 2011Assignee: Novozymes A/SInventors: Allan Svendsen, Stefan Minning
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Patent number: 8008241Abstract: An automatic dishwashing detergent composition comprising: a. at least 0.1 mg of active protease per gram of composition, wherein the protease is a variant of a protease that has at least 70% identity with the amino acid sequence of SEQ ID NO:1. wherein said variant comprise variations in one or more of the following positions: 32, 33, 48-54, 58-62, 94-107, 116, 123-133, 150, 152-156, 158-161, 164, 169, 175-186, 197, 198, 203-216 as compared with the protease in SEQ ID NO:1; and b. at least 0.05 mg of an active low temperature amylase per gram of composition.Type: GrantFiled: March 4, 2009Date of Patent: August 30, 2011Assignee: The Procter & Gamble CompanyInventor: Philip Frank Souter
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Publication number: 20110171718Abstract: The invention relates to modified polynucleotides encoding modified proteases, and methods for altering the production of proteases in microorganisms. In particular, the modified polynucleotides comprise one or more mutations that encode modified proteases having modifications of the pre-pro region that enhance the production of the active enzyme. The present invention further relates to methods for altering the production of proteases in microorganisms, such as Bacillus species.Type: ApplicationFiled: April 15, 2010Publication date: July 14, 2011Applicant: DANISCO US INC.Inventors: Alexander Pisarchik, Brian F. Schmidt
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Publication number: 20110159485Abstract: The present teachings relate to the extraction of nucleic acid from solid materials. Provided are useful compositions, methods and kits for obtaining nucleic acids from a solid biological sample or an adhesive material having a biological material adherent or embedded within the adhesive substrate. The extracted nucleic acid can be used in downstream applications such as genotyping, detection, quantification, and identification of the source of the biological material.Type: ApplicationFiled: September 14, 2010Publication date: June 30, 2011Applicant: LIFE TECHNOLOGIES CORPORATIONInventors: James Stray, Jason Yingjie Liu, Maxim Brevnov, Jaiprakash Shewale, Allison Holt
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Patent number: 7951574Abstract: The present invention relates to novel JP170 like subtilases from wild-type bacteria, hybrids thereof and to methods of construction and production of these proteases. Further, the present invention relates to use of the claimed subtilases in detergents, such as a laundry or an automatic dishwashing detergent.Type: GrantFiled: December 1, 2010Date of Patent: May 31, 2011Assignee: Novozymes A/SInventors: Preben Nielsen, Poul Erik Pedersen, Helle Outtrup
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Patent number: 7951573Abstract: The present invention relates to novel subtilases from wild-type strains of Bacillus, especially the Bacillus strains ZI344, EP655, P203, EP63, ZI120, ZI130, ZI1342 and ZI140, and to methods of construction and production of these proteases. Further, the present invention relates to use of the claimed subtilases in detergents, such as a laundry detergent or an automatic dishwashing detergent.Type: GrantFiled: November 18, 2010Date of Patent: May 31, 2011Assignee: Novozymes A/SInventors: Preben Nielsen, Martin Borchert
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Publication number: 20110104786Abstract: The present invention provides methods and compositions comprising at least one neutral metalloprotease enzyme in the relative absence of serine protease enzyme contaminants. In some embodiments, the neutral metalloprotease finds use in cleaning and other applications. In some particularly preferred embodiments, the present invention provides methods and composi-tions comprising Bacillus strains engineered to be deficient in multiple serine proteases, and their use in production of recombinant neutral metalloprotease(s).Type: ApplicationFiled: October 6, 2008Publication date: May 5, 2011Inventors: Anita Van Kimmenade, Edwin Lee, Andrew Shaw, Louise Wallace
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Publication number: 20110097783Abstract: The present invention relates to the identification of novel metallo-proteases (MP) in Gram-positive microorganisms. The present invention provides the nucleic acid and amino acid sequences for Bacillus MP. The present invention also provides host cells having a mutation or deletion of part or all of the gene encoding MP. The present invention also provides host cells further comprising nucleic acid encoding desired heterologous proteins such as enzymes. The present invention also provides cleaning compositions comprising an MP of the present invention.Type: ApplicationFiled: December 8, 2004Publication date: April 28, 2011Inventor: David A. Estell
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Publication number: 20110097440Abstract: The present invention relates to a method for the improvement of the short bite and texture parameters of bakery products by adding thereto at least one intermediate thermostable or thermostable serine or metallo-protease.Type: ApplicationFiled: May 14, 2009Publication date: April 28, 2011Applicant: PURATOS N.V.Inventors: Bruno Van Winckel, Fabienne Verte
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Publication number: 20110081454Abstract: The present invention provides methods and compositions comprising at least one neutral metalloprotease enzyme that has improved stability in the presence of a metal chelator. In some embodiments, the neutral metalloprotease finds use in cleaning and other applications comprising citrate. In some particularly preferred embodiments, the present invention provides methods and compositions comprising variant neutral metalloprotease(s) engineered to resist citrate-induced autolysis.Type: ApplicationFiled: October 23, 2008Publication date: April 7, 2011Inventors: Ronaldus W. J. Hommes, Amy D. Liu, Andrew Shaw, Louise Wallace