Bacteria Is Bacillus Patents (Class 435/221)
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Patent number: 7320887Abstract: The invention relates to novel alkaline protease variants. These variants have, when enumerating the alkaline protease from Bacillus lentus, variations in amino acid position 61, positions 199 and/or 211 and, optionally, at least one modification that contributes to the stabilization of the molecule, said modification preferably being point mutations in positions 3 and/or 4. Particularly preferred are variants S3T/V41/G61A/V199] and S3T/V41/G61A/V1991/L211D of B. lentus alkaline protease. The invention also relates to the possible use of these enzymes in diverse technical processes and, in particular, to detergents and cleansers containing these novel alkaline protease variants.Type: GrantFiled: April 30, 2004Date of Patent: January 22, 2008Assignee: Henkel Kommanditgesellschaft auf AktienInventors: Beatrix Kottwitz, Karl-Heinz Maurer, Roland Breves
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Patent number: 7306937Abstract: Novel enzyme variants including protease variants derived from the DNA sequences of naturally-occurring or re-combinant non-human proteases are disclosed. The variant proteases, in general, are obtained by in vitro modification of a precursor DNA sequence encoding the naturally-occurring or recombinant protease to generate the substitution of a plurality of amino acid residues in the amino acid sequence of a precursor protease. Such variant proteases have properties which are different from those of the precursor protease, such as altered wash performance. The substituted amino acid residue correspond to positions 27, 45, 170, 181, 251 and 271 of Bacillus amyloliquefaciens subtilisin. Additional variants comprising at least one additional substitution at a position selected from 1, 14, 49, 61, 87, 100, 102, 118, 128, 204 and 258 of Bacillus amyloliquefaciens subtilisin are also described.Type: GrantFiled: January 16, 2003Date of Patent: December 11, 2007Assignee: Genencor International, Inc.Inventors: Ayrookaran J. Poulose, David A Estell, James T Kellis, Jr., Richard R. Bott
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Patent number: 7297512Abstract: From fish egg skin produced by utilization of roe grains of various fish, constituent proteins thereof are enzymatically degraded to obtain peptides and amino acids, of which effective utilization is achieved. The invention provides a method for producing amino acids and peptides (useful as nutrient enhancers for foods) from fish egg skins which comprises treating cold water-washed fish egg skins with ozonized water at room temperature or below, subsequently, degrading the resultant product with a proteolytic enzyme produced by Bacillus subtilis, or further treating with a proteolytic enzyme produced by Aspergillus oryzae, to degrade myogenic fiber proteins (contractile proteins: myosins) in the fish egg skin, and then concentrating/drying the degraded solution.Type: GrantFiled: December 12, 2003Date of Patent: November 20, 2007Assignee: Fuji Bio Technology Institute Co., Ltd.Inventor: Kunihiko Kodaka
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Patent number: 7297528Abstract: An alkaline protease having the following properties; a gene encoding the same; a microorganism producing the same; and washing compositions containing the same; (i) acting over a broad pH value range of 4 to 13 and achieving, at pH 6 to 12, 80% or more the activity at the optimum pH value; (ii) when treated at 40° C. for 30 minutes, being stable over a pH value range of 6 to 11; (iii) having an isoelectric point of about 8.9 to 9.1; and (iv) having casein digesting activity that is not inhibited by oleic acid. The alkaline protease of the present invention is highly stable to various surface active agents and fatty acids, and exhibits high stability to oxidizing agents, and is therefore useful as an enzyme to be used in detergents for automatic dishwashers and laundry detergents, both containing bleaching components.Type: GrantFiled: February 24, 2004Date of Patent: November 20, 2007Assignee: Kao CorporationInventors: Mikio Takaiwa, Mitsuyoshi Okuda, Katsuhisa Saeki, Hiromi Kubota, Jun Hitomi, Yasushi Kageyama, Shitsuw Shikata, Masafumi Nomura
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Patent number: 7288401Abstract: The present invention relates to enzymes produced by mutating the genes for a number of subtilases and expressing the mutated genes in suitable hosts are presented. The enzymes exhibit improved wash performance in any detergent in comparison to their wild type parent enzymes.Type: GrantFiled: April 12, 2005Date of Patent: October 30, 2007Assignee: Novozymes A/SInventors: Peter Kamp Hansen, Peter Bauditz, Frank Mikkelsen, Kim Vilbour Andersen
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Patent number: 7241575Abstract: The present invention relates to the identification of novel metallo-proteases (MP) in Gram-positive microorganisms. The present invention provides the nucleic acid and amino acid sequences for Bacillus (MP). The present invention also provides host cells having mutation or deletion of part or all of the gene encoding MP. The present invention also provides host cells further comprising nucleic acid encoding desired heterologous proteins such as enzymes. The present invention also provides cleaning compositions comprising an MP of the present invention.Type: GrantFiled: August 25, 2004Date of Patent: July 10, 2007Assignee: Genecor International, Inc.Inventor: David A. Estell
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Patent number: 7217554Abstract: The present invention relates to isolated proteases of the RP-II type and variants of RP-II proteases exhibiting improved properties in comparison to the parent RP-II protease, DNA constructs and vectors coding for the expression of said proteases and variants, host cells capable of expressing the proteases and variants from the DNA constructs, as well as a method of producing them by cultivating said host cells. The proteases may advantageously be used as constituents in detergent compositions and additives, optionally in combination with other enzymes such as proteases, lipases, cellulases, amylases, peroxidases or oxidases.Type: GrantFiled: August 11, 2003Date of Patent: May 15, 2007Assignee: Novozymes A/SInventors: Mads Nørregaard-Madsen, Peter Rahbek Østergaard, Claus Bo Vøge Christensen, Søren Flensted Lassen
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Patent number: 7198903Abstract: The present invention relates to the identification of the molecular binding domains between presenilins and its substrates such as amyloid precursor protein and telencephalin. These binding domains can be efficiently used in drug screening assays to screen for compounds capable of modulating the interaction between presenilins and type I transmembrane proteins. The invention further relates to compounds capable of modulating the interaction.Type: GrantFiled: September 15, 2003Date of Patent: April 3, 2007Assignee: Vlaams Interuniversitair Instituut voor Biotechnologie VZWInventors: Bart De Strooper, Wim Annaert
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Patent number: 7192757Abstract: The present invention relates to subtilase variants having a reduced tendency towards inhibition by substances present in eggs, such as trypsin inhibitor type IV-0. In particular, the variants comprise at least one additional amino acid residue between positions 42–43, 51–56, 155–161, 187–190, 216–217, 217–218 or 218–219 (in BASBPN numbering). These subtilase variants are useful exhibiting excellent or improved wash performance on egg stains when used in e.g. cleaning or detergent compositions, such as laundry detergent compositions and dishwash composition, including automatic dishwash compositions. Also, isolated DNA sequences encoding the variants, expression vectors, host cells, and methods for producing and using the variants of the invention. Further, cleaning and detergent compositions comprising the variants are disclosed.Type: GrantFiled: July 2, 2004Date of Patent: March 20, 2007Assignee: Novozymes A/SInventors: Mads Nørregard-Madsen, Line Bloch Larsen, Peter Kamp Hansen
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Patent number: 7189553Abstract: The present invention relates to enzymes produced by mutating the genes for a number of subtilisin proteases and expressing the mutated genes in suitable hosts are presented. The enzymes exhibit improved wash performance in comparison to their wild type parent enzymes. The enzymes are well-suited for use in detergent compositions.Type: GrantFiled: December 6, 2002Date of Patent: March 13, 2007Assignee: Novozymes A/SInventors: Dorrit Aaslyng, Sven Branner, Sven Hastrup, Leif Norskov-Lauritsen, Ole Hvilsted Olsen, Merete Simonsen, Eric Castelijn, Maarten Robert Egmond, Johan Haverkamp, John David Marugg, Arnoldus Theodorus Anthonius Mooren
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Patent number: 7183071Abstract: The present invention relates to in vitro and ex vivo methods of screening for modulators, homologues, and mimetics of lethal factor mitogen activated protein kinase kinase (MAPKK) protease activity, as well as methods of treating cancer by administering LF to transformed cells.Type: GrantFiled: January 5, 2006Date of Patent: February 27, 2007Assignee: The United States of America as represented by the Department of Health and Human ServicesInventors: Nicholas Duesbery, Craig Webb, Stephen Leppla, George Vande Woude
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Patent number: 7172890Abstract: The present invention relates to oxidoreductase apoenzyme variants which are enzymatically inactive but have coenzyme-binding properties. Further, the present invention relates to DNA sequences encoding these oxidoreductase apoenzyme variants, expression vectors containing such DNA sequences and the use of these oxidoreductase apoenzyme variants in diagnostic applications.Type: GrantFiled: October 28, 2004Date of Patent: February 6, 2007Assignee: Roche Diagnostics GmbHInventors: Zhixin Shao, Joachim Hoenes, Carina Horn, Wolfgang-Reinhold Knappe, Rainer Schmuck
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Patent number: 7163815Abstract: The present invention is directed to compositions and methods comprising NAD(P)H oxidases, particularly bacterial oxidases, nucleic acids, recombinant plasmid vectors and recombinant proteins therein encoded, and host cells comprising the oxidases and nucleic acids. The present invention also comprises an isolated bacterial oxidase that oxidizes both NADH and NADPH. Methods for producing the enzymes and enzymatic reactions comprising use of NAD(P)H oxidases and products of such reactions are also disclosed.Type: GrantFiled: January 28, 2005Date of Patent: January 16, 2007Assignee: Georgia Tech Research CorporationInventors: Bettina Riebel-Bommarius, Andreas Bommarius, Phillip Gibbs, William Wellborn
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Patent number: 7129076Abstract: Novel protease variants derived from the DNA sequences of naturally-occurring or recombinant non-human proteases are disclosed. The variant proteases, in general, are obtained by in vitro modification of a precursor DNA sequence encoding the naturally-occurring or recombinant protease to generate the substitution of a plurality of amino acid residues in the amino acid sequence of a precursor protease. Protease variants are provided that contain substitutions of the amino acids at one or more residue positions so that the substitution alters the charge at that position to make the charge more negative or less positive compared to a precursor protease and thus the protease variant is more effective in a low detergent concentration system than a precursor protease.Type: GrantFiled: April 25, 2003Date of Patent: October 31, 2006Assignee: Genencor International, Inc.Inventors: Ayrookaran J. Poulose, Volker Schellenberger, James T. Kellis, Jr., Christian Paech, Joanne Nadherny, Donald P. Naki, Katherine D. Collier, Robert M. Caldwell
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Patent number: 7109016Abstract: The present invention relates to subtilases having a reduced tendency towards inhibition by substances present in eggs, such as the trypsin inhibitor type IV-0. These subtilases are useful exhibiting excellent or improved wash performance on egg stains when used in e.g. cleaning or detergent compositions, such as laundry detergent compositions and dishwash compositions, including automatic dishwash compositions.Type: GrantFiled: August 16, 2001Date of Patent: September 19, 2006Assignee: Novozymes A/SInventors: Helle Outtrup, Poul Erik Pedersen, Marianne Vind Sørensen
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Patent number: 7101698Abstract: The present invention relates to an alkaline protease having a prepro sequence, wherein the prepro sequence is a mutant sequence of the amino acid sequence of SEQ ID NO: 1 or an amino acid sequence having 80% or higher homology to the amino acid sequence of SEQ ID NO: 1, in which amino acid residues at (a) position 52, (b) position 75, and (c) position 142 of SEQ ID NO: 1, or amino acid residues at positions corresponding to these positions are substituted by the following amino acid residues: at position (a): aspartic acid or arginine, at position (b): alanine or arginine, and at position (c): lysine; and the alkaline protease, when in a mature form, has the amino acid sequence of SEQ ID NO: 2 or an amino acid sequence having a homology of 80% or higher to this amino acid sequence. The present invention also relates to, for example, a gene encoding the alkaline protease. According to the present invention, an alkaline protease increased in production can be produced.Type: GrantFiled: June 9, 2003Date of Patent: September 5, 2006Assignee: Kao CorporationInventors: Tsuyoshi Sato, Mitsuyoshi Okuda, Yasushi Takimura, Nobuyuki Sumitomo, Masafumi Nomura, Tohru Kobayashi
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Patent number: 7098017Abstract: The present invention relates to enzymes produced by mutating the genes for a number of subtilases and expressing the mutated genes in suitable hosts are presented. The enzymes exhibit improved autoproteolytic stability in comparison to their wild type parent enzymes.Type: GrantFiled: September 6, 2001Date of Patent: August 29, 2006Assignee: Novozymes A/SInventors: Claus von der Osten, Torben Halkier, Carsten Andersen, Peter Bauditz, Peter Kamp Hansen
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Patent number: 7081359Abstract: The present invention provides genetically engineered Bacillus strains that can secrete large amount of Bacillus proteases in the extracellular culture medium. More particularly, this invention relates to a process of producing recombinant protease molecules of Bacillus origin in a Bacillus subtilis strain 168, utilizing a strong prophage promoter.Type: GrantFiled: May 17, 2002Date of Patent: July 25, 2006Assignee: Enzymes Technology LimitedInventor: Boon Leong Lim
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Patent number: 7078216Abstract: The present invention relates to the identification of a novel metalloproteases in gram positive microorganisms. The present invention provides the nucleic acid and amino acid sequences for the metalloprotease. The present invention also provides host cells having a mutation or deletion of part or all of the gene encoding the metalloprotease. The present invention provides host cells which further comprises a nucleic acid encoding desired heterologous proteins such as enzymes. The present invention also provides cleaning compositions, animal feeds and compositions used to treat a textile that include the metalloprotease of the present invention.Type: GrantFiled: October 31, 2002Date of Patent: July 18, 2006Assignee: Genencor International, Inc.Inventor: David A. Estell
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Patent number: 7037503Abstract: The invention provides mutant forms of pore-forming toxins. These mutant toxins may be used in vaccines for the prevention of bacterial infection. Additionally, dominant negative mutants may be administered as therapeutics for the treatment of bacterial infection.Type: GrantFiled: May 4, 2001Date of Patent: May 2, 2006Assignee: President and Fellows of Harvard CollegeInventors: R. John Collier, Bret R. Sellman
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Patent number: 7033817Abstract: The present invention relates to the identification of a novel metalloproteases in gram positive microorganisms. The present invention provides the nucleic acid and amino acid sequences for the metalloprotease. The present invention also provides host cells having a mutation or deletion of part or all of the gene encoding the metalloprotease. The present invention provides host cells which further comprises a nucleic acid encoding desired heterologous proteins such as enzymes. The present invention also provides cleaning compositions, animal feeds and compositions used to treat a textile that include the metalloprotease of the present invention.Type: GrantFiled: June 21, 2002Date of Patent: April 25, 2006Assignee: Genencor International, Inc.Inventor: David A. Estell
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Patent number: 7026153Abstract: The present invention relates to protease subtilase enzyme, characterized by an insertion in at least one active site loop. The enzymes exhibit improved wash performance in a detergent in comparison to its parent enzyme if it is a subtilase variant.Type: GrantFiled: March 31, 2003Date of Patent: April 11, 2006Assignee: Novozymes A/SInventors: Peter Kamp Hansen, Peter Bauditz, Frank Mikkelsen
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Patent number: 6960462Abstract: Acid-stable proteases of the subtilisin family, their use in animal feed, feed-additives and feed compositions containing such proteases, and methods for the treatment of vegetable proteins using such proteases.Type: GrantFiled: February 8, 2001Date of Patent: November 1, 2005Assignee: DSM IP Assets B.VInventors: Carsten Sjoeholm, Peter Rahbek Oestergaard, Anna-Marie Kluenter
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Patent number: 6955907Abstract: A novel alkaline protease VapK suitable for a laundry detergent is disclosed. The gene vapk coding for the protease VapK, the recombinant plasmids containing said gene, and the transformed V. metshnikovii KS1 (pSBCm) with said recombinant plasmid are also disclosed. In addition, a process for producing the protease VapK is disclosed.Type: GrantFiled: January 14, 2000Date of Patent: October 18, 2005Assignee: Cheil Jedang CorporationInventors: Ghee Hong Jin, Hyoung Suk Kim, Hyune Mo Rho, Hyune Whan Lee
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Patent number: 6946128Abstract: The present disclosure relates to subtilisin protease conjugate comprising a protease moiety and one or more addition moieties. Each addition moiety is covalently attached to an epitope protection position of the protease moiety. The protease conjugates have decreased immunogenicity relative to a parent protease. The present disclosure further relates to cleaning and personal care compositions comprising the protease conjugates.Type: GrantFiled: July 18, 2000Date of Patent: September 20, 2005Assignee: The Procter & Gamble CompanyInventors: Donn Nelton Rubingh, David John Weisgerber, Paul Elliott Correa
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Patent number: 6939703Abstract: The present invention relates to novel amylolytic enzymes having improved characteristics for the use in starch degradation, in textile or paper desizing and in household detergent compositions. The disclosed ?-amylases show surprisingly improved properties with respect to the activity level and the combination of thermostability and a higher activity level. These improved properties make them more suitable for the use under more acidic or more alkaline conditions. The improved properties allow also the reduction of the Calcium concentration under application conditions without a loss of performance of the enzyme.Type: GrantFiled: July 18, 2002Date of Patent: September 6, 2005Assignee: Genencor International, Inc.Inventors: Jan M. Van Der Laan, Wolfgang Aehle
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Patent number: 6929939Abstract: The present invention relates to novel protein variants that exhibit reduced allergenicity when compared to the parental proteins. Also included are DNA molecules that encode the novel variants, host cells comprising the DNA and methods of making proteins less allergenic.Type: GrantFiled: March 22, 2002Date of Patent: August 16, 2005Assignees: Genencor International, Inc., The Procter & Gamble CompanyInventors: David A. Estell, Grant C. Ganshaw, Fiona A. Harding, Edmund A. Larenas, Ayrookaran J. Poulose, Elizabeth E. Sikorski, Elliott P. Russell
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Patent number: 6927055Abstract: Novel protease variants derived from the DNA sequences of naturally-occurring or recombinant non-human proteases are disclosed. The variant proteases, in general, are obtained by in vitro modification of a precursor DNA sequence encoding the naturally-occurring or recombinant protease to generate the substitution of a plurality of amino acid residues in the amino acid sequence of a precursor protease. Such variant proteases have properties which are different from those of the precursor protease, such as altered wash performance. The substituted amino acid residue correspond to positions 62, 212, 230, 232, 252 and 257 of Bacillus amyloliquefaciens subtilisin.Type: GrantFiled: August 27, 2002Date of Patent: August 9, 2005Assignee: Genencor International, Inc.Inventors: Ayrookaran J. Poulose, Volker Schellenberger, James T. Kellis, Jr., Christian Paech, Joanne Nadherny, Donald P. Naki, Katherine D. Collier, Robert M. Caldwell, André C. Baeck
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Patent number: 6921657Abstract: The present invention relates to enzymes produced by mutating the genes for a number of subtilases and expressing the mutated genes in suitable hosts are presented. The enzymes exhibit improved wash performance in any detergent in comparison to their wild type parent enzymes.Type: GrantFiled: January 3, 2003Date of Patent: July 26, 2005Assignee: Novozymes A/SInventors: Peter Kamp Hansen, Peter Bauditz, Frank Mikkelsen, Kim Vilbour Andersen
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Patent number: 6908757Abstract: The present invention relates to variants of serine proteases having decreased immunogenicity relative to their corresponding wild-type proteases. More particularly, the present invention relates to variants having a modified amino acid sequence of a wild-type amino acid sequence, wherein the modified amino acid sequence comprises a deletion and, optionally, a substitution of one or more specifically identified positions corresponding to subtilisin BPN?. The invention further relates to mutant genes encoding such variants and cleaning and personal care compositions comprising such variants.Type: GrantFiled: March 26, 1999Date of Patent: June 21, 2005Assignee: The Procter & Gamble CompanyInventors: Donn Nelton Rubingh, Elizabeth Ellen Sikorski, Paul Elliott Correa
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Patent number: 6908991Abstract: The present invention relates to mutations of a subtilisin gene which result in changes in the chemical characteristics of subtilisin enzymes. Mutations at specific nucleic acids of the subtilisin gene result in amino acid substitutions and consequently, altered enzyme function. Some of these mutant enzymes exhibit physical properties advantageous to industrial applications, particularly in the detergent industry, providing subtilisin which is more stable to oxidation, possesses greater protease activity, and exhibits improved washability.Type: GrantFiled: December 6, 2002Date of Patent: June 21, 2005Assignee: Novozymes A/SInventors: Sven Hastrup, Sven Branner, Fanny Norris, Steffen Bjorn Petersen, Leif Norskov-Lauridsen, Villy Johannes Jensen, Dorrit Aaslyng
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Patent number: 6902922Abstract: New subtilisin homologues (both nucleic acids and proteins) are provided. Compositions which include these new proteins, recombinant cells, shuffling methods involving the new homologues, antibodies to the new homologues, and methods of using the homologues are also provided.Type: GrantFiled: April 2, 2001Date of Patent: June 7, 2005Assignees: Novozymes A/S, Maxygen, Inc.Inventors: Jon E. Ness, Mark Welch, Lorraine J. Giver, Joel R. Cherry, Torben V. Borchert, Willem P. C. Stemmer, Jeremy Minshull
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Patent number: 6897049Abstract: The present invention relates to a novel methods and compositions for producing hyper and hypo allergenic compositions. Specifically, the present invention comprises neutralizing or reducing the ability of T-cells to recognize epitopes and thus prevent sensitization of an individual to the protein. Alternatively, T-cell epitopes are mutated to produce increased immunogenic reactions.Type: GrantFiled: January 23, 2001Date of Patent: May 24, 2005Assignee: Genencor International, Inc.Inventors: David A. Estell, Fiona A. Harding
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Patent number: 6893855Abstract: The present invention relates to subtilase variants having a reduced tendency towards inhibition by substances present in eggs, such as trypsin inhibitor type IV-0. In particular, the variants comprise at least one additional amino acid residue between positions 42-43, 51-56, 155-161, 187-190, 216-217, 217-218 or 218-219 (in BASBPN numbering). These subtilase variants are useful exhibiting excellent or improved wash performance on egg stains when used in e.g. cleaning or detergent compositions, such as laundry detergent compositions and dishwash composition, including automatic dishwash compositions. Also, isolated DNA sequences encoding the variants, expression vectors, host cells, and methods for producing and using the variants of the invention. Further, cleaning and detergent compositions comprising the variants are disclosed.Type: GrantFiled: October 12, 2001Date of Patent: May 17, 2005Assignee: Novozymes A/SInventors: Mads Nørregaard-Madsen, Line Bloch Larsen, Peter Kamp Hansen
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Patent number: 6893835Abstract: The present invention relates to in vitro and ex vivo methods of screening for modulators, homologues, and mimetics of lethal factor mitogen activated protein kinase kinase (MAPKK) protease activity, as well as methods of treating cancer by administering LF to transformed cells.Type: GrantFiled: March 5, 2002Date of Patent: May 17, 2005Assignee: The United States of America as represented by the Department of Health and Human ServicesInventors: Nicholas Duesbery, Craig Webb, Stephen Leppla, George Vande Woude
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Patent number: 6863918Abstract: An improved infant formula resulting in reduced constipation, abdominal discomfort and gastrointestinal problems, comprises at least one protein component having a phosphorus content of less than 0.75 g P/100 g protein, and at least one lipid component that can be easily digested by an infant. Preferably, it further comprises at least one prebiotic component, and at least one viscosity-improving component. The protein fraction of the formula is preferably a hydrolysate prepared by hydrolysing a protein starting material, especially a whey protein with a combination of at least one endo- and at least one exoproteinase.Type: GrantFiled: December 13, 2000Date of Patent: March 8, 2005Assignee: N.V. NutriciaInventors: Jacob Geert Bindels, Antonie Van Baalen, Robert Johan Joseph Hageman, Peti Huybers, Liliane Marie-Rose Louisa Dominique Dumon
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Patent number: 6849441Abstract: There are provided hyperthermostable proteases having an amino acid sequences represented by SEQ ID NOs: 1, 3 and 5 of the Sequence Listing or functional equivalents thereof and hyperthermostable protease genes encoding those hyperthermostable protease. There is also disclosed a process for preparation of a hyperthermostable protease by culturing a transformant containing the gene.Type: GrantFiled: April 24, 2001Date of Patent: February 1, 2005Assignee: Takara Shuzo Co., LTDInventors: Hikaru Takakura, Mio Morishita, Katsuhiko Yamamoto, Masanori Mitta, Kiyozo Asada, Susumu Tsunasawa, Ikunoshin Kato
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Patent number: 6846656Abstract: The present invention provides a process for economically producing N-acetylneuraminic acid without using expensive materials such as pyruvic acid and phosphoenolpyruvic acid. The process comprises: allowing (i) a culture of a microorganism having N-acetylneuraminic acid aldolase activity or N-acetylneuraminic acid synthetase activity, or a treated matter of the culture, (ii) a culture of a microorganism capable of producing pyruvic acid or a treated matter of the culture, or a culture of a microorganism capable of producing phosphoenolpyruvic acid or a treated matter of the culture, (iii) N-acetylmannosamine, and (iv) an energy source which is necessary for the formation of pyruvic acid or phosphoenolpyruvic acid to be present in an aqueous medium to form and accumulate N-acetylneuraminic acid in the aqueous medium; and recovering N-acetylneuraminic acid from the aqueous medium.Type: GrantFiled: August 25, 2000Date of Patent: January 25, 2005Assignee: Kyowa Hakko Kogyo Co., Ltd.Inventors: Satoshi Koizumi, Kazuhiko Tabata, Tetsuo Endo, Akio Ozaki
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Patent number: 6846664Abstract: The present invention relates to a novel strain of alkalothermophilic Bacillus sp. isolated from a hot spring at Vajeshwari, District Thane, The State of Maharashtra, India and deposited at American Type Culture Centre (ATCC), bearing accession No. PTA 972, said strain of Bacillus sp. having the following characteristics (i) aerobic, (ii) gram positive, (iii) motile, (iv) spore forming, (v) capable of growing in a alkaline medium at pH 8-10, and (vi) exhibiting negative reaction towards production of indole, hydrogen, sulfide, ammonia and urease and positive reaction for hydrolysis of starch, production of catalase, hydrolysis of casein and reduction of nitrate.Type: GrantFiled: June 12, 2002Date of Patent: January 25, 2005Assignee: Council for Scientific and Industrial ResearchInventors: Chandravanu Dash, Sangita Uday Phadtare, Absar Ahmad, Vasanti Vishnu Deshpande, Mala Balchandra Rao
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Patent number: 6838425Abstract: The present invention relates to cleaning compositions comprising a protease variant.Type: GrantFiled: January 15, 2002Date of Patent: January 4, 2005Assignee: The Procter & Gamble CompanyInventors: Chanchal Kumar Ghosh, André Cesar Baeck, Ryohei Ohtani, Alfred Busch, Michael Stanford Showell, Ayrookaran J. Poulose, Volker Schellenberger, James T. Kellis, Jr., Christian Paech, Joanne Nadherny, Donald P. Naki, Katherine D. Collier, Robert M. Caldwell
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Publication number: 20040253703Abstract: The present invention relates to novel aminopeptidase derived from Bacillus licheniformis, a gene encoding the aminopeptidase, an expression vector containing the gene, a cell transformant transfected with the expression vector and a process for preparing a natural type protein using thereof. More particularly, the present invention relates to a gene encoding aminopeptidase which is cloned and manufactured using the recombinant DNA technique, an expression vector containing the gene, a cell transformant transfected with the expression vector and a recombinant aminopeptidase which is necessary to produce recombinant human growth hormone in a natural type protein and can be expressed in a high yield more stably and advantageously, compared with conventional methods for the purification.Type: ApplicationFiled: December 19, 2003Publication date: December 16, 2004Inventors: Young-Phil Lee, Seung-won Lee, Chul-ho Jung, Hyung-Cheol Kim, Song-Yong Choi, Jin-Suk Kim, Hyun-Sik Kim, Jung-Woo Seo
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Publication number: 20040253665Abstract: The present invention relates to a novel enzyme that allows peptide to be produced easily, inexpensively and at high yield without going through a complex synthesis method. More particularly, the present invention provides a novel enzyme that catalyzes a peptide-producing reaction from a carboxy component and an amine component, a microbe that produces the enzyme, and a method for inexpensive production of peptides using this enzyme or microbe. The novel enzyme that efficiently produces peptide was discovered from a newly discovered microbe belonging to the genus Empedobacter, and a method was found that allows peptides to be produced inexpensively and easily.Type: ApplicationFiled: April 8, 2004Publication date: December 16, 2004Applicant: AJINOMOTO CO., INC.Inventors: Kenzo Yokozeki, Sonoko Suzuki, Seiichi Hara, Satoshi Katayama
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Publication number: 20040253704Abstract: The present invention relates to a surface expression vector having pgsBCA, a gene coding poly-gamma-glutamate synthetase and a method for expression of target protein at the surface of microorganism using the vector. The vector, in which foreign genes are inserted, transforms microorganisms and makes foreign proteins expressed stably on the surface of microorganisms.Type: ApplicationFiled: February 12, 2004Publication date: December 16, 2004Inventors: Moon-Hee Sung, Seung-Pyo Hong, Jong-Su Lee, Chang-Min Jung, Chul-Joong Kim, Kenji Soda, Makoto Ashiuchi
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Patent number: 6831053Abstract: The present invention relates to bleaching compositions comprising a protease variant.Type: GrantFiled: April 20, 2000Date of Patent: December 14, 2004Assignee: The Procter & Gamble CompanyInventors: Chanchal Kumar Ghosh, André Cesar Baeck, Ryohei Ohtani, Alfred Busch, Michael Stanford Showell, Ayrookaran J. Poulose, Volker Schellenberger, James T. Kellis, Jr., Christian Paech, Joanne Nadherny, Donald P. Naki, Katherine D. Collier, Robert M. Caldwell
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Patent number: 6815193Abstract: Novel protease variants derived from the DNA sequences of naturally-occurring or recombinant non-human proteases are disclosed. The variant proteases, in general, are obtained by in vitro modification of a precursor DNA sequence encoding the naturally-occurring or recombinant protease to generate the substitution of a plurality of amino acid residues in the amino acid sequence of a precursor protease. Such variant proteases have properties which are different from those of the precursor protease, such as altered wash performance.Type: GrantFiled: November 2, 2001Date of Patent: November 9, 2004Assignee: Genencor International, Inc.Inventors: Ayrookaran J. Poulose, Volker Schellenberger, James T. Kellis, Jr., Christian Paech, Joanne Nadherny, Donald P. Naki, Katherine D. Collier, Robert M. Caldwell, André C. Baeck
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Patent number: 6808913Abstract: The present invention relates to mutations of a subtilisin gene which result in changes in the chemical characteristics of subtilisin enzymes. Mutations at specific nucleic acids of the subtilisin gene result in amino acid substitutions and consequently, altered enzyme function. Some of these mutant enzymes exhibit physical properties advantageous to industrial applications, particularly in the detergent industry, providing subtilisin which is more stable to oxidation, possesses greater protease activity, and exhibits improved washability.Type: GrantFiled: November 27, 2002Date of Patent: October 26, 2004Assignee: Novozymes A/SInventors: Sven Hastrup, Sven Branner, Fanny Norris, Steffen Bjørn Petersen, Leif Nørskov-Lauridsen, Villy Johannes Jensen, Dorrit Aaslyng
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Patent number: 6780629Abstract: The present invention relates to subtilase enzymes of the I-S1 and I-S2 sub-groups having an additional amino acid in the active site loop (c) region from positions 125 to 132. The variant subtilases of the present invention exhibit improved wash performance in a detergent in comparison to its parent enzyme.Type: GrantFiled: September 13, 2002Date of Patent: August 24, 2004Assignee: Novozymes A/SInventors: Peter Kamp Hansen, Peter Bauditz, Frank Mikkelsen, Kim Vilbour Andersen, Carsten Andersen, Mads Norregaard-Madsen
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Patent number: 6777218Abstract: The present invention relates to novel subtilases having an improved wash performance on egg stains. The present invention also relates to isolated nucleic acid sequences encoding the subtilases, nucleic acid constructs, recombinant expression vectors, host cells comprising the nucleic acid construct, and methods for producing and using the subtilases of the invention. Further, the present invention relates to cleaning and detergent compositions comprising the subtilase enzymes of the invention as well as to use of such enzymes in detergent compositions and for removal of egg stains.Type: GrantFiled: March 23, 2000Date of Patent: August 17, 2004Assignees: Novozymes A/S, Maxygen, IncInventors: Frank Mikkelsen, Tina Sejersgård Fanø, Jon E. Ness, Mark D. Welch, Lorraine J. Giver, Jeremy S. Minshull, Torben V. Borchert, Joel Cherry
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Patent number: 6773907Abstract: The present invention relates to subtilase enzymes of the I-S1 and I-S2 sub-groups having an additional amino acid in the active site loop (b) region from positions 95 to 103. The variant subtilases of the present invention exhibit improved wash performance in a detergent in comparison to its parent enzyme.Type: GrantFiled: September 12, 2002Date of Patent: August 10, 2004Inventors: Peter Kamp Hansen, Peter Bauditz, Frank Mikkelsen, Kim Vilbour Andersen, Carsten Andersen, Mads Norregaard-Madsen
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Patent number: 6770469Abstract: A novel enzyme which has an activity to release side chain carboxyl groups and ammonia from a protein by acting upon side chain amido groups in the protein. This invention relates to a method for the production of an enzyme, which comprises culturing in a medium a strain that belongs to a bacterium classified into Cytophagales or Actinomycetes and has the ability to produce an enzyme having a property to deamidate amido groups in protein, thereby effecting production of said enzyme, and subsequently collecting said enzyme from the culture mixture. It also relates to a method for the modification of protein making use of a novel enzyme which directly acts upon amido groups in protein as well as to an enzyme which has a property to deamidate amido groups in protein and a gene which encodes said enzyme.Type: GrantFiled: February 27, 2001Date of Patent: August 3, 2004Assignee: Amano Pharmaceutical Co., Ltd.Inventors: Shotaro Yamaguchi, Akira Matsuura