Bacillus (e.g., B. Subtilis, B. Thuringiensis, Etc.) Patents (Class 435/252.5)
  • Patent number: 10851387
    Abstract: Compositions and methods for conferring pesticidal activity to bacteria, plants, plant cells, tissues and seeds are provided. Compositions comprising a coding sequence for a toxin polypeptide are provided. The coding sequences can be used in DNA constructs or expression cassettes for transformation and expression in plants and bacteria. Compositions also comprise transformed bacteria, plants, plant cells, tissues, and seeds. In particular, isolated toxin nucleic acid molecules are provided. Additionally, amino acid sequences corresponding to the polynucleotides are encompassed, and antibodies specifically binding to those amino acid sequences. In particular, the present invention provides for isolated nucleic acid molecules comprising nucleotide sequences encoding the amino acid sequence shown in SEQ ID NO:4-7, or the nucleotide sequence set forth in SEQ ID NO:1, 2, or 3, as well as variants and fragments thereof.
    Type: Grant
    Filed: April 3, 2018
    Date of Patent: December 1, 2020
    Assignee: BASF AGRICULTURAL SOLUTIONS SEED, US LLC
    Inventor: Kimberly S. Sampson
  • Patent number: 10687529
    Abstract: The present application relates generically to compositions used for controlling agricultural pests and, in a particular, to compositions usable for combating nematodes. The application presents a feasible alternative to the use of nematotoxic compounds that are harmful to the environment and that may damage human health. This alternative includes a nematotoxic composition for controlling nematodes that may be used on plants, parts of plants and on the soil. The respective technology results from unexpected results achieved from experiments carried out with extracts of the plant Canavalia ensiformis, resulting in an unheard-of nematotoxic composition comprising a specific combination of substances that are naturally found in the extract and that act synergistically.
    Type: Grant
    Filed: December 26, 2013
    Date of Patent: June 23, 2020
    Assignees: EMPRESA BRASILEIRA DE PESQUISA AGROPECUÁRIA-EMBRAPA, THE UNIVERSITY OF MELBOURNE
    Inventors: Thales Lima Rocha, Raphael Garcia de Sousa Evaristo, Maria Fátima Grossi de Sá, Djair Dos Santos de Lima e Souza, Maria Cristina Mattar da Silva, Vera Lúcia Perussi Polez, Ute Roessner, Tony Bacic
  • Patent number: 10597627
    Abstract: The present invention provides an apparatus and a method which can be used for producing an organic substance effectively from a synthesis gas obtained from a waste incinerator. The apparatus 1 for producing an organic substance from waste, comprises a synthesis gas generation furnace 11, a fermenter 13, and a nutrient feeder. The synthesis gas generation furnace 11 generates a synthesis gas by partial oxidation of waste. The fermenter 13 contains a microorganism which produces an organic substance from the synthesis gas. The nutrient feeder 12 feeds a solid or liquid nutrient to the fermenter 13 when an amount of the synthesis gas supplied to the fermenter 13 is insufficient.
    Type: Grant
    Filed: July 27, 2015
    Date of Patent: March 24, 2020
    Assignee: SEKISUI CHEMICAL CO., LTD.
    Inventors: Kanetomo Satou, Norihide Nishiyama, Kokoro Hamachi, Tetsuya Ishii
  • Patent number: 10358654
    Abstract: Compositions having pesticidal activity and methods for their use are provided. Compositions include isolated and recombinant polypeptides having pesticidal activity, recombinant and synthetic nucleic acid molecules encoding the polypeptides, DNA constructs and vectors comprising the nucleic acid molecules, host cells comprising the vectors, and antibodies to the polypeptides. Polynucleotide sequences encoding the polypeptides can be used in DNA constructs or expression cassettes for transformation and expression in organisms of interest. The compositions and methods provided are useful for producing organisms with enhanced pest resistance or tolerance. Transgenic plants and seeds comprising a nucleotide sequence that encodes a pesticidal protein of the invention are also provided. Such plants are resistant to insects and other pests. Methods are provided for producing the various polypeptides disclosed herein, and for using those polypeptides for controlling or killing a pest.
    Type: Grant
    Filed: December 16, 2016
    Date of Patent: July 23, 2019
    Assignee: AGBIOME, INC.
    Inventors: Jessica Parks, Kira Bulazel Roberts, Rebecca E. Thayer
  • Patent number: 10117841
    Abstract: The present invention includes novel chemical formulations having antimicrobial activity and their methods of use thereof. In some embodiments, the formulation further comprises at least one fungus.
    Type: Grant
    Filed: July 2, 2014
    Date of Patent: November 6, 2018
    Assignee: ECOPLANET ENVIRONMENTAL LLC
    Inventors: Gary A. Strobel, Bryan Blatt
  • Patent number: 10092008
    Abstract: The present invention relates to a method for increasing the vigor and/or crop yield of agricultural plants under essentially non-existent pathogen pressure, wherein the plants, the plant propagules, the seed of the plants and/or a locus where the plants are growing or are intended to grow are treated with an effective amount of a composition comprising a Bacillus subtilis strain with Accession No. NRRL B-21661, a mutant of the strain having all the identifying characteristics of the strain, or a cell-free extract thereof; wherein the vigor and/or crop yield of agricultural plants under essentially non-existent pathogen pressure is increased and the increase in vigor is characterized by less fertilizer needed.
    Type: Grant
    Filed: April 21, 2016
    Date of Patent: October 9, 2018
    Assignee: Bayer CropScience LP
    Inventors: Markus Frank, David Ernest Silva
  • Patent number: 9976158
    Abstract: A process and apparatus is provided which are effective for improving CO mass transfer. The process includes introducing syngas into a reactor vessel through a gas sparger located below a liquid level in the reactor vessel. The syngas being introduced at a flow rate effective for maintaining a pressure inside of the reactor vessel of at least about 1 psig. An agitation energy of about 0.01 to about 12 kWatts/m3 medium is provided. The process is effective for providing a volumetric CO mass transfer coefficient of about 100 to about 1500 per hour.
    Type: Grant
    Filed: May 15, 2012
    Date of Patent: May 22, 2018
    Inventors: Peter Simpson Bell, Ching-Whan Ko
  • Patent number: 9781938
    Abstract: The present invention relates to a method of eliciting plant immune responses based on seed priming by using the heat (high temperature)-treated culture solution of Bacillus spp, and the high pressure-sterilized culture solution of Bacillus sp. strains PB69 and 1628 which have been selected in the present invention exhibits an activity of eliciting induced systemic resistance similar to BTH, and also has an effect for inducing promoted plant growth and controlling plant diseases, and thus it is expected to contribute to enhance the crop productivity.
    Type: Grant
    Filed: November 13, 2013
    Date of Patent: October 10, 2017
    Assignee: KOREA RESEARCH INSTITUTE OF BIOSCIENCE AND BIOTECHNOLOGY
    Inventors: Choong Min Ryu, Geun Cheol Song
  • Patent number: 9315874
    Abstract: The present invention provides a mutant strain of Bacillus subtilis SFA-H31 and includes an optimized method for producing of acetoin using this strain. The advantage of this method to produce acetoin has been defined by its high-yield without mixture with diacetyl or 2,3-butanediol, both of which are usually accompanied with acetoin in other strain. This Bacillus subtilis mutant strain has been deposited in China General Microbiological Culture Collection (CGMCC) and the accession number is CGMCC No. 1869, in which the process of fermentation for acetoin production is composed of (1) strain activation, (2) seed culture, (3) fermentation, (4) quantification of substrates and products. Based on current method, the concentration of acetoin in the ferment broth can reach to 35-55 g/L and the conversion rate of glucose to acetoin is in the range of 40-50%.
    Type: Grant
    Filed: January 8, 2008
    Date of Patent: April 19, 2016
    Assignee: Shandong Food Fermentation Industry Research & Design Institute
    Inventors: Jianjun Liu, Xiangying Zhao, Jiaxiang Zhang, Yanjun Tian, Yanlei Han, Li Han
  • Patent number: 9238822
    Abstract: The present invention is directed to controlling pest infestation by inhibiting one or more biological functions in an invertebrate pest. The invention discloses methods and compositions for use in controlling pest infestation by feeding one or more different recombinant double stranded RNA molecules to the pest in order to achieve a reduction in pest infestation through suppression of gene expression. The invention is also directed to methods for making transgenic plants that express the double stranded RNA molecules, and to particular combinations of transgenic pesticidal agents for use in protecting plants from pest infestation.
    Type: Grant
    Filed: September 6, 2011
    Date of Patent: January 19, 2016
    Assignee: Monsanto Technology LLC
    Inventors: James A. Baum, Larry A. Gilbertson, David K. Kovalic, Thomas J. LaRosa, Maolong Lu, Tichafa R. I. Munyikwa, James K. Roberts, Wei Wu, Bei Zhang
  • Patent number: 9205116
    Abstract: Disclosed herein are microbiocidal compositions that include spore-forming strains of Bacillus. The compositions may be utilized to treat or prevent disease in aquatic animals such as farmed fish or crustaceans.
    Type: Grant
    Filed: April 30, 2012
    Date of Patent: December 8, 2015
    Assignee: Auburn University
    Inventors: Jeffery Terhune, Mark Liles, Joseph Kloepper
  • Patent number: 9144588
    Abstract: Biologically pure cultures of Bacillus subtilis were isolated from the pericarp of nixtamalized corn. The Bacillus microorganisms were characterized and found to produce novel peptides which have antimicrobial activity. The Bacillus microorganisms are active against Gram-positive bacteria and may be used as an agent to prevent spoilage in foods. The Bacillus microorganisms may be included in probiotic food compositions to help maintain healthy gastrointestinal flora and modulate animal digestion.
    Type: Grant
    Filed: September 14, 2011
    Date of Patent: September 29, 2015
    Assignee: INVESTIGACION DE TECNOLOGIA AVANZADA, S.A. DE C.V.
    Inventors: Felipe A. Rubio, Manuel J. Rubio, Roberto Contreras, J. Fernando Ramirez
  • Publication number: 20150147768
    Abstract: The invention provides a culture plate made from a polymer incorporating a culture component releasable into culture media in the well, methods of culturing a microorganisms in the culture plate, and a methods of making the culture plate.
    Type: Application
    Filed: September 20, 2013
    Publication date: May 28, 2015
    Applicant: Danisco US Inc
    Inventors: Jimmy Chan, Rachel E. Muir, William Throndset
  • Publication number: 20150140550
    Abstract: Methods for aiding in the diagnosis of disorders including, but not limited to, PDDs (Pervasive Development Disorders), Dysautonomic disorders, Parkinson's disease and SIDS (Sudden Infant Death Syndrome). In one aspect, a diagnosis method comprises analyzing a stool sample of an individual for the presence of a biological marker (or marker compound) comprising one or more pathogens, which provides an indication of whether the invidual has, or can develop, a disorder including, but not limited to, a PDD, Dysautonomia, Parkinsons disease and SIDS. Preferably, the presence of one or more pathogens is determined using a stool immunoassay to determine the presence of antigens in a stool sample, wherein such antigens are associated with one or more pathogens including, but not limited to, Giardia, Cryptosporidium, E. histolytica, C. difficile, Adenovirus, Rotavirus or H. pylori.
    Type: Application
    Filed: October 30, 2014
    Publication date: May 21, 2015
    Inventor: Joan M. Fallon
  • Patent number: 9023339
    Abstract: A method for controlling nematodes in or on a plant, a plant part, and/or a locus for plant growth is provided, the method comprising applying to a plant, plant part, or locus for plant growth in need of protection from said nematodes an effective amount of Bacillus pumilus, a mutant of Bacillus pumilus, or at least one metabolite of Bacillus pumilus. In particular, the Bacillus pumilus strain may be strain QST2808.
    Type: Grant
    Filed: November 2, 2012
    Date of Patent: May 5, 2015
    Assignee: Bayer CropScience LP
    Inventors: Nathan Royalty Reed, Philip Thomas Varghese
  • Patent number: 9005954
    Abstract: The present invention relates to compositions and methods to identify novel bacteria and metabolites derived therefrom. More specifically, the invention describes a novel method to isolate bacteria producing metabolites of interest from environmental samples. Particularly, the invention discloses a method to select rare antibiotic producing bacteria. The invention can be used from any sample and allows the isolation of bacteria having e.g., pharmaceutical or agrochemical interest.
    Type: Grant
    Filed: January 18, 2010
    Date of Patent: April 14, 2015
    Assignees: Deinove, Centre National de la Recherche Scientifique
    Inventors: Jean-Paul Leonetti, Stéphanie Texier
  • Patent number: 8999318
    Abstract: A composition is provided that includes Bacillus subtilis 2084 (NRRL B-50013) or a strain having all of the identifying characteristics of the Bacillus subtilis 2084 (NRRL B-50013), B. subtilis 27 (NRRL B-50105) or a strain having all of the identifying characteristics of the B. subtilis 27 (NRRL B-50105), and B. licheniformis 21 (NRRL B-50134) or a strain having all of the identifying characteristics of the B. licheniformis 21 (NRRL B-50134). Animal bedding that includes the Bacillus strains is also provided, as well as a method of making the animal bedding. Also provided are methods of controlling odors from animal waste. A method of making a composition including the Bacillus strains is also provided.
    Type: Grant
    Filed: March 1, 2013
    Date of Patent: April 7, 2015
    Assignee: Dupont Nutrition Biosciences APS
    Inventors: Marianne Cain Bellot, Keith J. Mertz, Thomas G. Rehberger
  • Publication number: 20150087614
    Abstract: Provided herein are methods for inducing cell death in a gram positive bacterium and methods for reducing the proliferation of a gram positive bacterium that include contacting a gram positive bacterium with a dye (e.g., an azo dye) and a TarO inhibitor. Also provided are methods of treating a subject having a gram positive bacterial infection that include administering to a subject having a gram positive bacterial infection a dye (e.g., an azo dye) and a TarO inhibitor. Also provided are compositions containing a dye (e.g., an azo dye) and a TarO inhibitor, and kits containing at least one of these compositions.
    Type: Application
    Filed: March 14, 2013
    Publication date: March 26, 2015
    Inventors: Michael S. Gilmore, Takashi Suzuki
  • Publication number: 20150079059
    Abstract: This invention relates to a sterilization agent for livestock bedding or a composting accelerator for organic waste or livestock bedding, comprising at least one Bacillus coagulans thermophilic bacterium or a combination of the at least one Bacillus coagulans thermophilic bacterium and at least one Bacillus subtilis mesophilic bacterium, as an active ingredient, wherein the Bacillus coagulans thermophilic bacterium as a preferable bacterium is viable at a temperature of 40 to 60° C. and the Bacillus subtilis mesophilic bacterium is viable at a temperature of 30 to 50° C., and the mesophilic bacterium also has a sterilizing effect on harmful bacteria in bedding; and to a method for sterilizing livestock bedding or a method for accelerating composting of organic waste or livestock bedding by using these agents each as an active ingredient.
    Type: Application
    Filed: September 15, 2014
    Publication date: March 19, 2015
    Applicant: CALPIS CO., LTD.
    Inventors: Taisuke Uesugi, Gentaro Yasuda
  • Publication number: 20150079661
    Abstract: The present invention is a bacteria and nutrient delivery composition containing bran. The subject composition is preferably made in the form of a tablet that is structurally stable without being excessively hard. The tablets preferably have a configuration that reduces the likelihood of premature shearing in tableting presses or jamming in feeder devices for biomass generators. Methods of manufacturing the bacterial delivery composition in a structurally stable form that maintains bacterial viability are also provided.
    Type: Application
    Filed: April 25, 2014
    Publication date: March 19, 2015
    Applicant: NCH Corporation
    Inventors: Judith G. Pruitt, Robert Clarence Pearce, III, John Ray Roheim
  • Patent number: 8962291
    Abstract: The invention relates to malodor controlling bacteria and related methods and compositions for the control and prevention of malodor.
    Type: Grant
    Filed: February 15, 2012
    Date of Patent: February 24, 2015
    Assignees: Novozymes Bioag A/S, Henkel AG & Co. KGAA
    Inventors: Sarah C. McHatton, I. Michelle Williams, Alejandro Penaloza-Vazquez
  • Publication number: 20150037302
    Abstract: The present invention relates to a method of producing biosurfactants, such as surfactin, comprising culturing at least one biosurfactant-producing microbe in a liquid culture medium comprising vinasse as a carbon source. Methods of using the crude biosurfactant containing culture broth in tertiary oil recovery and as antibacterial compositions in tertiary oil recovery are also described. Methods of using the culture broth residue after isolation of the biosurfactants as fertilizer and compositions for this use are also described.
    Type: Application
    Filed: January 25, 2013
    Publication date: February 5, 2015
    Inventors: Michael Paul Bralkowski, Sarah Ashley Brooks, Stephen M. Hinton, David Matthew Wright, Shih-Hsin Yang
  • Publication number: 20150037307
    Abstract: The present invention relates to methods of improving the environment within a poultry farming facility including reducing ammonia production in a poultry facility, inhibiting urease enzymes in poultry litter, reducing levels of pathogenic bacteria in poultry litter, improving productivity of poultry farms, reducing or preventing pododermatitis in poultry reared in mass production poultry facilities and controlling pests in poultry litter. Compositions, suitable for use in such methods, comprising at least one microorganism of the genus Bacillus and at least one biosurfactant wherein the biosurfactant is present in an amount of 2 mg/L to 7000 mg/L are also described.
    Type: Application
    Filed: January 25, 2013
    Publication date: February 5, 2015
    Inventors: Michael Paul Bralkowski, Sarah Ashley Brooks, Stephen M. Hinton, David Matthew Wright, Shih-Hsin Yang
  • Publication number: 20150004673
    Abstract: The present invention relates to natural crude salt formulations arid their cost-effective preparation. In particular, the invention discloses the fractionation of seawater into three fractions which can thereafter be suitably combined to reconstitute seawater in its pristine form for its various applications such as growth of marine microbes, flora and fauna in a marine environment, especially where such environments have to be created in locations far removed from the sea and/or where there is a requirement to modify the composition of seawater to better its performance. The fractions can also be blended appropriately to exclude the major constituent, namely sodium chloride, and thereby be useful for mineralization/re-mineralization of waters which are deficient in mineral nutrients such as calcium, magnesium, potassium, sulphate and bicarbonate including rain water and desalinated waters obtained through thermal/RO desalination.
    Type: Application
    Filed: December 27, 2012
    Publication date: January 1, 2015
    Inventors: Pushpito Kumar Ghosh, Sumesh Chandra Upadhyay, Sandhya Chandrikaprasad Mishra, Vadakke Puthoor Mohandas, Divesh Narayan Srivastava, Vinod Kumar Shahi, Rahul Jasvantrai Sanghavi, Sreekumaran Thampy, Babulal Surabhai Makwana, Imran Pancha, Ruma Pal, Ramkrishna Sen
  • Publication number: 20140370146
    Abstract: A bacillus composition characterized by fast germination and outgrowth in bile salts (simulated gut environment) and by producing a compound of interest. The bacillus composition may be used as supplement in animal feed where it has a probiotic (health promoting) effect and increases the digestion and availability of nutrients from animal feeds.
    Type: Application
    Filed: May 21, 2014
    Publication date: December 18, 2014
    Applicant: Chr. Hansen A/S
    Inventors: Inge KNAP, Ane KNARREBORG, Thomas Dyrmann LESER, Lund BENTE, Mette Dines CANTOR, Patrick DERKX
  • Publication number: 20140335262
    Abstract: [Object] To obtain natto having reduced viscosity and natto odor. [Solving Means] Provided are Bacillus subtilis AZ-5512 strain (FERM P-22135) and natto produced by using the strain.
    Type: Application
    Filed: September 11, 2012
    Publication date: November 13, 2014
    Applicant: AZUMA FOODS CO., LTD.
    Inventors: Noriei Okuhata, Yoshihiro Ushiku, Yoshiko Hayashi, Keiko Yoshihara, Sugio Watanabe
  • Publication number: 20140315716
    Abstract: In one aspect, the present invention relates to novel Bacillus strains ENV 734 (NRRL B-50800), ENV 735 (NRRL B-50801), ENV 736 (NRRL B-50802), and ENV 737 (NRRL B-50803). These strains possess a high degree of homology with both Bacillus simplex and Bacillus butanolivorans, but unexpectedly exhibit desirable high salt tolerance as well as low temperature growth and urease production. In another aspect, the present invention relates to compositions comprising at least one of such strains and an acceptable carrier, and methods of preparing the compositions. Such compositions are suitable for use in industrial, agricultural, aquacultural, environmental, wastewater treatment and/or probiotic applications. Methods for enhancing the growth of a plant propagative material and methods for treating wastewater are also provided.
    Type: Application
    Filed: April 17, 2014
    Publication date: October 23, 2014
    Applicant: Envera, LLC
    Inventors: Michael Matheny, Zivile Panaviene
  • Publication number: 20140308733
    Abstract: The present application relates to a method for inducing germination of a spore forming bacterium, specifically, a method for inducing germination of a spore forming bacterium, comprising: using one or more compounds having a phenolic backbone to induce germination of a spore forming bacterium selected from the group consisting of the genus Bacillus, the genus Geobacillus, and the genus Aeribacillus; to a germination inducer for a spore forming bacterium; and to a kit for use in germination of a spore forming bacterium.
    Type: Application
    Filed: September 6, 2012
    Publication date: October 16, 2014
    Inventors: Toki Nishiyama, Nobuhisa Ashida, Gentaro Yasuda, Yukie Tadano
  • Patent number: 8852917
    Abstract: The present invention relates to a new bacteriocin, to microbial strains which can produce it and to uses of the bacteriocin and the strains. The bacteriocin is effective against Clostridium difficile and Listeria monocytogenes amongst other organisms.
    Type: Grant
    Filed: November 28, 2008
    Date of Patent: October 7, 2014
    Assignees: University College Cork, TEAGASC, The Agriculture and Food Development Authority
    Inventors: Colin Hill, Mary Rea, Paul Ross
  • Publication number: 20140295482
    Abstract: Methods and compositions for enhancing or promoting germination of bacterial spores, and yeasts are disclosed herein. The composition of the present invention comprises an extract obtained from banana or any member belonging to the genus Musa that may be used alone or in a growth medium to promote and enhance germination of bacterial spores, growth of bacterial, yeast, and fungal cell cultures.
    Type: Application
    Filed: November 2, 2012
    Publication date: October 2, 2014
    Inventor: Mark Lyte
  • Publication number: 20140273160
    Abstract: The present invention relates to novel bacteria strains that can be used in biological control against mosquito larvae (Culex spp.). The protein obtained from a novel B. sphaericus spp. isolates with the invention is used as larvicide, the step of isolating the protein at product obtaining stage is eliminated. By means of the invention, thee bacterial strains (MIB 5,6,7) investigated for biological control of mosquitoes are effective in both polluted and fresh water.
    Type: Application
    Filed: July 5, 2012
    Publication date: September 18, 2014
    Applicant: YEDITEPE UNIVERSITESI
    Inventors: Fikrettin Sahin, Gulengul Duman, Muge Yazici
  • Patent number: 8835145
    Abstract: The invention relates to a thrombolytic enzyme referred to as Thrombinase having a molecular weight of 31,000 to 32,000. Such a thrombolytic enzyme can be used for dissolving blood clots. The process comprises culturing a filtrate of Bacillus sphaericus sero type H5a 5b, removing the cell, subjecting the cell supernatant to filtration, salting out the retentate, subjecting the precipitate to dialysis, reprecipitating the precipitate and then reconstituting in buffer and finally decolorizing, purifying and dialyzing.
    Type: Grant
    Filed: October 12, 2007
    Date of Patent: September 16, 2014
    Assignees: National Research Development Corporation, India and Malladi Drugs Pharmaceuticals Ltd.
    Inventors: Subrahamanyam Chivukula Sekar, Sundaramurthy Suresh Babu, Sita Mahadevan
  • Patent number: 8821854
    Abstract: The present invention describes compositions and methods for inhibiting microbial infections associated with the use of sanitary products, such as diapers, bandages, sanitary napkins, tampons, and the like. The present invention comprises providing for use a sanitary product containing an effective amount of a viable, non-pathogenic, lactic acid-producing bacteria, such as Bacillus coagulans, or an extracellular product thereof, useful for inhibiting growth of parasites and pathogens on the epithelial tissue in contact with the sanitary product during use of the product. The present invention also provides for enhancing biodegradation of sanitary products after use and disposal. Also described herein are methods using the product and systems containing the compositions.
    Type: Grant
    Filed: April 10, 2006
    Date of Patent: September 2, 2014
    Assignee: Ganeden Biotech, Inc.
    Inventors: Sean Farmer, Andrew R. Lefkowitz
  • Patent number: 8815548
    Abstract: The invention features methods for producing isoprene from cultured cells using a feedback-resistant mevalonate kinase polypeptide, such as an archaeal mevalonate kinase polypeptide. The resulting isoprene compositions may have increased yields and/or purity of isoprene.
    Type: Grant
    Filed: December 28, 2012
    Date of Patent: August 26, 2014
    Assignees: Danisco US Inc., The Goodyear Tire & Rubber Company
    Inventors: Zachary Q. Beck, Anthony R. Calabria, Michael C. Miller, Alex T. Nielsen, Dmitrii V. Vaviline
  • Patent number: 8802079
    Abstract: A bacillus composition characterized by fast germination and outgrowth in bile salts (simulated gut environment) and by producing a compound of interest. The bacillus composition may be used as supplement in animal feed where it has a probiotic (health promoting) effect and increases the digestion and availability of nutrients from animal feeds.
    Type: Grant
    Filed: December 16, 2009
    Date of Patent: August 12, 2014
    Assignee: Chr. Hansen A/S
    Inventors: Inge Knap, Ane Knarreborg, Thomas Dyrmann Leser, Lund Bente, Mette Dines Cantor, Patrick Derkx
  • Patent number: 8802389
    Abstract: The subject invention relates in part to the surprising and unexpected discovery that insects that are resistant to Bacillus thuringiensis Cry toxins have measurably altered alkaline phosphatase (ALP) activity as compared to insects that are susceptible to Cry toxins. This and other surprising discoveries reported herein have broad implications in areas such as managing and monitoring the development of insect resistance to B.t. toxins. For example, the subject invention provides a simple and fast assay (enzymatic or otherwise) for detecting ALP activity levels and thereby monitoring the development of resistance by insects to crystal protein insect toxins. There was no prior motivation or suggestion to go about resistance monitoring using this simple and easy approach.
    Type: Grant
    Filed: October 17, 2011
    Date of Patent: August 12, 2014
    Assignee: University of Georgia Research Foundation, Inc.
    Inventors: Michael J. Adang, Rebecca McNall, Juan Luis Jurat Fuentes
  • Patent number: 8801864
    Abstract: An aqueous composition is disclosed, comprising at least one strain of Class 1 Bacillus bacterial spores, selected from Bacillus Cirulans, Bacillus Megaterium, Bacillus Licheniformis, Bacillus Pumilus, Bacillus Sphaericus, Bacillus Subtilis sub species 10144 and Bacillus Subtilis sub species 8646, a terpene and one or more surfactants. The terpene is preferably d-limonene. A method for cleaning surfaces is also disclosed, which method comprises deploying an aqueous composition comprising at least one strain of Class 1 Bacillus bacterial spores, selected from Bacillus Cirulans, Bacillus Megaterium, Bacillus Lichenforms, Bacillus Pumilus, Bacillus Sphaericus, Bacillus Subtilis sub species 10144 and Bacillus Subtilis sub species 8646, a terpene and one or more surfactants to a surface.
    Type: Grant
    Filed: September 30, 2011
    Date of Patent: August 12, 2014
    Assignee: Cleveland Biotech Limited
    Inventor: Anthony Brooke
  • Patent number: 8802420
    Abstract: Some HBL and NHE enterotoxins are known to cause food-borne diseases in humans. Enterotoxin-deficient mutants of member strains of the Bacillus cereus group that do not produce HBL, HBLa1, HBLa2, or NHE enterotoxins are disclosed. Enterotoxin-deficient mutants are suitable for use as biocontrol agents. Methods for making the mutants and for using the mutants are described.
    Type: Grant
    Filed: June 7, 2011
    Date of Patent: August 12, 2014
    Assignee: Wisconsin Alumni Research Foundation
    Inventors: Jo Emily Handelsman, Amy Klimowicz, Changhui Guan
  • Publication number: 20140220662
    Abstract: In one aspect, the present invention is directed to a dried intimate mixture comprising a bacteria spore and a germinative compound, and methods for preparing the intimate mixture. In another aspect, this invention is directed to a composition comprising such an intimate mixture. The invention also relates to methods for increasing the germination, growth, metabolism, and/or enzyme activity of a bacteria spore comprising preparing an intimate mixture of a bacteria spore and a germinative compound.
    Type: Application
    Filed: February 6, 2014
    Publication date: August 7, 2014
    Applicant: Envera, LLC
    Inventor: Tommie Eugene Hashman
  • Publication number: 20140212376
    Abstract: The present invention relates to the field of biotechnology, and particularly relates to a bionematicide composition broad spectrum action on phytopathogenic nematodes which comprises one or more bacterial strains isolated from Chilean soil belonging to the genus Bacillus, and the method of use of this composition for the protection of plants against nematode attack.
    Type: Application
    Filed: January 30, 2014
    Publication date: July 31, 2014
    Inventors: Paulo Valdes Escobar, Eduardo Cuevas Donoso, Gustavo Prats Lobos
  • Publication number: 20140206048
    Abstract: The present invention provides compositions and methods for the use of oil-containing materials as feedstocks for the production the bioproducts by biofermentation. In one preferred embodiment, surfactants are not used in compositions and the methods of the invention. In one preferred embodiment the oil-containing feedstocks are the by-products of other industrial processes including microbial, plant and animal oil processing.
    Type: Application
    Filed: January 22, 2014
    Publication date: July 24, 2014
    Applicant: Glycos Biotechnologies, Inc.
    Inventors: Daniel J. Monticello, Ryan Black, Paul Campbell
  • Publication number: 20140189895
    Abstract: The present invention provides a method of artificially repressing gene expression, which is simpler to design than conventional methods (the RNAi, ribozyme and antisense methods) and which allows for easier confirmation of the effect. A method of inhibiting the translation reaction of a target gene, comprising cutting out a part of the poly(A) tail and/or 3?-terminal sequence of the target mRNA is provided.
    Type: Application
    Filed: July 17, 2012
    Publication date: July 3, 2014
    Applicants: YOSHINDO INC., PUBLIC UNIVERSITY CORPORATION YOKOHAMA CITY UNIVERSITY, TOKYO INSTITUTE OF TECHNOLOGY
    Inventors: Tadashi Wada, Kei Takeda, Hiroshi Handa
  • Publication number: 20140179528
    Abstract: The present invention relates to the Bacillus sp. strains AT-332 (NITE BP-1095) and AT-79 (NITE BP-1094) isolated from nature; and a plant disease control agent, a nematode control agent and a plant growth promoter containing the strains as active bacteria. The Bacillus sp. strains AT-332 and AT-79 strain are effective in controlling both a wide range of various plant diseases and nematode-damage and capable of promoting the growth of useful plants, due to a culture containing a secondary metabolite of the strains or cultivated and isolated live bacteria of the strains being introduced to a plant body or to the culture soil.
    Type: Application
    Filed: May 21, 2012
    Publication date: June 26, 2014
    Applicant: SDS BIOTECH K.K.
    Inventors: Yusuke Amaki, Keijitsu Tanaka, Motoki Tanaka, Akitomo Takahashi
  • Patent number: 8748153
    Abstract: A biosorbent for removing cationic and/or anionic metals from aqueous solutions, and a process for the production of the biosorbent. The biosorbent includes bacterial aggregates of Bacillus sp. VCHB-10, deposited as NRRL-B-30881, and treated with polyethyleneimine and glutaraldehyde. Among the metals in their cationic form, the following are considered: cations of Ag, Al, Au, Co, Cd, Cu, Cr, Fe, Hg, Mn, Ni, Pb, Pd, Pt, U, Th and Zn. Among the metals in their anionic form, the following are considered: anions of As, Cr and Mo. Removal or recovery of metals from wastewater using the biosorbent is also described.
    Type: Grant
    Filed: May 11, 2011
    Date of Patent: June 10, 2014
    Assignees: Universidad de Chile, Biotecnologia del Agua Ltda.
    Inventors: Davor Cotoras Tadic, Pabla Viedma, Juan Manuel Gajardo Sandoval
  • Patent number: 8748158
    Abstract: Designed to afford a novel microorganism promoting gastric juice secretion and having platelet increasing activity, and to afford a pharmaceutical agent composed of a product secreted by the novel microorganism. The novel microorganism, international deposit number: NITE BP-295, belongs to the species Bacillus pumilus, and is characterized by taking either form of coccus and bacillus, and makes figure-eight movement. The novel microorganism of the present invention is the novel microorganism having the gene represented by SEQ ID No: 1, and an object of the present invention is to afford the microorganism and a gastric juice secretion-promoting composition composed of the product secreted by the novel microorganism.
    Type: Grant
    Filed: August 10, 2007
    Date of Patent: June 10, 2014
    Inventor: Yoshichika Kameyama
  • Publication number: 20140154750
    Abstract: The present invention is directed to a strain of Bacillus subtilis and applications using the strain to raise the efficiency and/or yield of generation of glucose produced by the hydrolysis of cellulose.
    Type: Application
    Filed: May 30, 2013
    Publication date: June 5, 2014
    Applicant: NATIONAL CENTRAL UNIVERSITY
    Inventors: SHIR-LY HUANG, SHENG-HSIN CHOU
  • Patent number: 8741625
    Abstract: Thermophilic microorganisms having a deodorizing ability for short chain fatty acids, which are the offensive odor components, are provided. Disclosed are thermophilic microorganisms having a deodorizing ability for short chain fatty acids, which belong to Bacillus licheniformis.
    Type: Grant
    Filed: November 9, 2011
    Date of Patent: June 3, 2014
    Assignee: Toyota Jidosha Kabushiki Kaisha
    Inventors: Nobuki Tada, Hibiki Matsushita, Toshiaki Kimura
  • Publication number: 20140144838
    Abstract: The present invention provides a bio-augmentation composition for improving the hydrocarbon degradation efficiency of effluent treatment plant for hydrocarbon degradation in wastewater generated from hydrocarbon processing industry and a method thereof. The composition comprises a synergistic combination of selective microorganisms to develop a consortium enabling effective degradation of hydrocarbons present in wastewater and converting thereof into harmless and environment friendly substances. The invention also provides for the said microorganisms and their isolations.
    Type: Application
    Filed: April 2, 2012
    Publication date: May 29, 2014
    Applicant: INDIAN OIL CORPORATION LTD.
    Inventors: Manoj Kumar, Mahendra Pratap Singh, Ashok Kumar Tiwari, Deepak Kumar Tuli, Ravinder Kumar Malhotra, Anand Kumar
  • Publication number: 20140147873
    Abstract: In various embodiments a system is provided that displays heterologous proteins on the surface of a Gram-positive microorganism. In certain embodiments the system displays proteins using a sortase transpeptidase to covalently anchor proteins to the cell wall of the microbe. Novel bacterial strains are provided to exploit this system to display cellulase enzymes and multi-enzyme complexes on the surface of Gram-positive microorganisms (e.g., Bacillus subtilis) through their non-covalent interaction with a scaffoldin protein that is covalently anchored to the cell wall by the sortase transpeptidase. The surface displayed protein complexes contain enzymes capable of degrading cellulose into its component sugars at accelerated rates as compared to solutions of purified enzymes.
    Type: Application
    Filed: February 29, 2012
    Publication date: May 29, 2014
    Applicant: THE REGENTS OF THE UNIVERSITY OF CALIFORNIA
    Inventors: Robert T. Clubb, Timothy Anderson
  • Patent number: 8728460
    Abstract: A biological soil nutrient system that combines beneficial soil fungi and bacteria in a growth promoting nutrient medium, embedded in an inorganic porous ceramic particle for direct delivery during soil aerification to the rhizosphere of adventitious plants, including sports turf, landscape and agricultural applications.
    Type: Grant
    Filed: September 9, 2010
    Date of Patent: May 20, 2014
    Assignee: Profile Products, L.L.C.
    Inventor: Kevin S. Spittle