Abstract: Glycoside hydrolases having at least two different hydrolytic activities are provided. In one embodiment, an isolated recombinant hydrolase having at least two activities selected from a group including asparagine derivatives, glutamine derivatives, and histidine derivatives is provided. Further, a method of generating free sugars from a mixture comprising asparagine derivatives, glutamine derivatives, and histidine derivatives is provided.
Type:
Application
Filed:
May 1, 2012
Publication date:
June 5, 2014
Applicants:
Sandia Corporation, The Regents of the University of California
Inventors:
Zhiwei Chen, Gregory D. Friedland, Swapnil R. Chhabra, Dylan C. Chivian, Blake A. Simmons
Abstract: The present invention relates to engineered multivalent and multispecific binding proteins, methods of making, and specifically to their uses in the prevention and/or treatment of acute and chronic inflammatory and other diseases.
Type:
Application
Filed:
February 10, 2014
Publication date:
June 5, 2014
Applicant:
AbbVie, Inc.
Inventors:
Chengbin Wu, Tariq Ghayur, Richard W. Dixon, Jochen G. Salfeld
Abstract: The present invention provides novel promoter and terminator sequences for use in gene expression in eukaryotic cells, such as algal cells. The invention further provides expression cassettes comprising a promoter, as described herein, operably linked to a gene. The invention further provides expression vectors and host eukaryotic cells, such as algal cells, for expressing a protein encoded by the gene; and methods for stably transforming eukaryotic algae such as Tetraselmis with transgenes.
Type:
Application
Filed:
December 4, 2012
Publication date:
June 5, 2014
Applicant:
ExxonMobil Research and Engineering Company
Inventors:
Jane C. Schneider, Soyan Lieberman, Bo Liu, Eric R. Moellering, John H. Verruto, Amanda Skaggs
Abstract: Testosteronan, a heparosan analog having the structure [-4-D-GlcUA-?1,4-D-GlcNAc-?1-]n, is produced by testosteronan synthase, a single protein that is a dual-action catalyst that utilizes UDP-GlcUA and UDP-GlcNAc to synthesize a polysaccharide having the structure [-4-D-GlcUA-?1,4-D-GlcNAc-?1-]n.
Type:
Application
Filed:
January 31, 2012
Publication date:
June 5, 2014
Applicant:
The Board of Regents of the University of Oklahoma
Abstract: The invention provides non-naturally occurring microbial organisms having a butadiene pathway. The invention additionally provides methods of using such organisms to produce butadiene.
Type:
Application
Filed:
October 21, 2013
Publication date:
June 5, 2014
Applicant:
Genomatica, Inc.
Inventors:
Mark J. Burk, Anthony P. Burgard, Jun Sun, Robin E. Osterhout, Priti Pharkya
Abstract: The present invention provides a novel biosynthetic pathway for the production of isoprene from 3-methyl-2-buten-1-ol or 2-methyl-3-buten-2-ol. Further embodiments provide non-naturally occurring microorganism that have been modified to produce isoprene from 3-methyl-2-buten-1-ol or 2-methyl-3-buten-2-ol and methods of producing isoprene using said microorganism.
Type:
Grant
Filed:
May 15, 2013
Date of Patent:
June 3, 2014
Assignee:
Glycos Biotechnologies, Inc.
Inventors:
Paul Campbell, Sebastian Bredow, Huaijin Zhou, Stephanie Doneske, Daniel J. Monticello
Abstract: The present invention provides a novel biosynthetic pathway for the production of isoprene from 3-methyl-2-buten-1-ol or 2-methyl-3-buten-2-ol. Further embodiments provide non-naturally occurring microorganism that have been modified to produce isoprene from 3-methyl-2-buten-1-ol or 2-methyl-3-buten-2-ol and methods of producing isoprene using said microorganism.
Type:
Grant
Filed:
May 15, 2013
Date of Patent:
June 3, 2014
Assignee:
Glycos Biotechnologies, Inc.
Inventors:
Paul Campbell, Sebastian Bredow, Huaijin Zhou, Stephanie Doneske, Daniel J. Monticello
Abstract: The present invention provides Relaxin fusion proteins, wherein a linker connects the carboxy-terminus of Relaxin with a proteinaceous half-life extending moiety and the linker comprises a protease cleavage site. Therefore, the invention provides Relaxin fusion polypeptides with extended half-life whereby the fusion protein by itself serves as a depot for release of the biologically active Relaxin. Furthermore, the invention provides nucleic acid sequences encoding the foregoing fusion polypeptides, vectors containing the same, cells expressing the Relaxin fusion polypeptides, pharmaceutical compositions and medical use of such fusion polypeptides.
Abstract: The present disclosure relates to variant CBH I polypeptides that have reduced product inhibition, and compositions, e.g., cellulase compositions, comprising variant CBH I polypeptides. The variant CBH I polypeptides and related compositions can be used in variety of agricultural and industrial applications. The present disclosure further relates to nucleic acids encoding variant CBH I polypeptides and host cells that recombinantly express the variant CBH I polypeptides.
Type:
Application
Filed:
October 6, 2011
Publication date:
May 29, 2014
Applicant:
BP Corporation North America Inc.
Inventors:
Justin T. Stege, Alexander Varvak, John Poland, Chris S. Lyon, Shaun Healey, Peter Luginhuhl
Abstract: The present invention concerns polypeptides comprising a variant Fc region. More particularly, the present invention concerns Fc region-containing polypeptides that have altered effector function as a consequence of one or more amino acid modifications in the Fc region thereof.
Abstract: The present invention relates to variants of alpha amylase. The present invention also relates to polynucleotides encoding the variants; nucleic acid constructs, vectors, and host cells comprising the polynucleotides; and methods of using the variants.
Type:
Application
Filed:
July 6, 2012
Publication date:
May 29, 2014
Applicant:
NOVOZYMES A/S
Inventors:
Tomoko Matsui, Aki Tomiki, Guillermo Coward-Kelly
Abstract: The present invention relates to pesticide preparations and methods based on RNAi technology. The invention discloses target genes (fragments) useful in the control of Lepidoptera insects. Nucleic acid inhibitors or hosts expressing the nucleic acid inhibitors, based on the nucleic acid sequences of these target genes, can effectively kill Lepidoptera insects. The invention also discloses applications using the nucleic acid inhibitors or hosts expressing the nucleic acid inhibitors.
Type:
Application
Filed:
March 19, 2012
Publication date:
May 29, 2014
Applicant:
Shanghi Institutes for Biological Sciences, Chines Academy of Science
Abstract: A non-naturally occurring microbial organism having an isopropanol, 4-hydroxybutryate, or 1,4-butanediol pathway includes at least one exogenous nucleic acid encoding an isopropanol, 4-hydroxybutryate, or 1,4-butanediol pathway enzyme expressed in a sufficient amount to produce isopropanol, 4-hydroxybutryate, or 1,4-butanediol. The aforementioned organisms are cultured to produce isopropanol, 4-hydroxybutryate, or 1,4-butanediol.
Type:
Application
Filed:
June 18, 2013
Publication date:
May 29, 2014
Applicant:
Genomatica, Inc.
Inventors:
John D. Trawick, Mark J. Burk, Anthony P. Burgard
Abstract: The present invention relates to isolated polypeptides having endoglucanase activity, catalytic domains, cellulose binding domains and polynucleotides encoding the polypeptides, catalytic domains or cellulose binding domains. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides, catalytic domains or cellulose binding domains.
Type:
Application
Filed:
April 26, 2012
Publication date:
May 29, 2014
Applicant:
Novozymes, Inc.
Inventors:
Nikolaj Spodsberg, Brett McBrayer, Pierre Cassland
Abstract: The subject matter of the invention is an electroconducting nanowire, comprising an amyloid fibre which results from the self-assembling of peptides comprising a prion domain or a derivative of this domain and which is functionalized by electron transporting peptides, each comprising amino acids bound to one or more atoms of iron. Its subject matter is also a method of manufacturing this nanowire as well as the uses of said nanowire. Uses: manufacture of sensors for the detection of chemical or biological species, of nano-batteries, of radio-identification systems, of molecular memory systems, etc.
Type:
Application
Filed:
March 6, 2012
Publication date:
May 29, 2014
Applicant:
COMMISSARIAT A L'ENERGIE ATOMIQUE ET AUX ENERGIES ALTERNATIVES
Inventors:
Vincent Forge, Christophe Horvath, Marc Fontecave, Nicolas Duraffourg
Abstract: The present invention relates to novel polynucleotide sequences comprising genes that encode novel lipolytic enzymes, as well as functional equivalents of the gene of the acid sequences with high homology thereto. The invention also relates to methods of using these lipolytic enzymes in industrial processes, for example in the diary or baking industry.
Type:
Application
Filed:
December 6, 2013
Publication date:
May 29, 2014
Applicant:
DSM IP ASSETS B.V.
Inventors:
Jan Metske VAN DER LAAN, Yulia M. EFIMOVA, Karin TURK, Albertus Alard VAN DIJK, Margot Elisabeth Francoise SCHOONEVELD-BERGMANS, Arie Gerrit TERDU, Arjen SEIN
Abstract: The present invention provides methods and compositions of variant polypeptides having isoprene synthase activity with improved solubility. In particular, the present invention provides isoprene synthase variant for increased isoprene production in recombinant host cells.
Type:
Grant
Filed:
October 26, 2012
Date of Patent:
May 27, 2014
Assignees:
Danisco US Inc., The Goodyear Tire & Rubber Company
Abstract: A polynucleotide encoding a biosensor polypeptide comprising a modified circularly-permuted thermostable luciferase and a linker linking the C-terminal portion of the thermostable luciferase to the N-terminal portion of the thermostable luciferase. The modified circularly-permuted thermostable luciferase is modified relative to a parental circularly-permuted thermostable luciferase. The linker contains a sensor region capable of interacting with a target molecule in a cell. The modified circularly-permuted thermostable luciferase has an enhanced response after interaction of the biosensor with the target molecule relative to the parental circularly-permuted thermostable luciferase in the presence of the target molecule. Alternatively, the modified circularly-permuted thermostable luciferase has an enhanced response after interaction of the biosensor with the target molecule relative to the modified circularly-permuted thermostable luciferase in the absence of the target molecule.
Type:
Grant
Filed:
May 11, 2011
Date of Patent:
May 27, 2014
Assignee:
Promega Corporation
Inventors:
Brock Binkowski, Braeden Butler, Lance P. Encell, Frank Fan, Brad Hook, Paul Otto, Gediminas Vidugiris, Susan Wigdal, Kristopher Zimmerman
Abstract: The invention relates to binding compounds that specifically bind to human TSLP, as well as uses thereof, e.g., in the treatment of inflammatory disorders and allergic inflammatory response.
Abstract: The invention provides means and methods for producing one or more Ig-like molecules in a single host cell. Novel CH3 mutations enabling the production of monospecific and/or bispecific Ig-like molecules of interest are also provided.
Type:
Application
Filed:
November 15, 2013
Publication date:
May 22, 2014
Applicant:
MERUS B.V.
Inventors:
Cornelis A. DE KRUIF, Linda Johanna Aleida HENDRIKS, Ton LOGTENBERG
Abstract: The present invention relates to methods for producing a polypeptide having biological activity, comprising: (a) cultivating a fungal host cell in a medium conducive for the production of the polypeptide, wherein the fungal host cell comprises a first polynucleotide encoding the polypeptide operably linked to a second polynucleotide encoding a variant signal peptide or a variant prepropeptide; and (b) isolating the secreted polypeptide having biological activity from the cultivation medium.
Abstract: The invention pertains to/the field of production of natural products and, in particular, in the field of production of cornexistin and hydroxycornexistin. It provides polynucleotides encoding polypeptides involved in the biosynthesis of cornexistin and hydroxycornexistin as well as vectors and recombinant microorganisms comprising such polynucleotides. Also provided are methods for the production of natural products, in particular methods for the production of cornexistin and hydroxycornexistin, using such polynucleotides and polpeptides encoded therein, as well as vectors and recombinant microorganisms comprising such polynucleotides and polypeptides.
Type:
Application
Filed:
November 19, 2013
Publication date:
May 22, 2014
Applicant:
BASF SE
Inventors:
Oskar Zelder, Birgit Hoff, Hartwig Schröder, Andrea Molt, Holger Hartmann, Klaus Ditrich, Michael Breuer, Rüdiger Reingruber, Jakob Weber
Abstract: The present invention demonstrates the utility of carbonic acid amides such as urea or its derivatives, carbamates, carbodiimides & thiocarbamides as nitrogenous supplements in fermentation media for production of recombinant proteins to achieve enhanced bioconversion rates and peptides like insulin and insulin analogues, exendin and enzymes such as lipase using methanol inducible fungal expression systems such as Pichia.
Abstract: This invention relates generally to enzymes, polynucleotides encoding the enzymes having isomerase activity, e.g., racemase activity, e.g., amino acid racemase activity, alanine racemase activity, and/or epimerase activity, and/or catalyze the re-arrangement of atoms within a molecule, catalyze the conversion of one isomer into another, catalyze the conversion of an optically active substrate into a raceme, which is optically inactive, catalyze the interconversion of substrate enantiomers, catalyze the stereochemical inversion around the asymmetric carbon atom in a substrate having only one center of asymmetry, catalyze the stereochemical inversion of the configuration around an asymmetric carbon atom in a substrate having more than one asymmetric center, and/or catalyze the racemization of amino acids.
Type:
Application
Filed:
August 14, 2013
Publication date:
May 22, 2014
Applicant:
Verenium Corporation
Inventors:
David P. WEINER, Ellen G. BURKE, Peter LUGINBUHL, Analia BUENO, Joslin M. CUENCA, Mervyn L. DE SOUZA, Sherry KOLLMANN
Abstract: The invention provides a method for producing 3?-phosphoadenosine 5?-phosphosulfate (PAPS), the method including subjecting ATP to sulfation and phosphorylation by use of adenosine 5?-triphosphate sulfurylase (ATPS) and adenosine 5?-phosphosulfate kinase (APSK), wherein an adenosine 5?-triphosphate (ATP) supply/regeneration system including adenosine 5?-monophosphate (AMP), polyphosphate, polyphosphate-driven nucleoside 5?-diphosphate kinase (PNDK), and polyphosphate:AMP phosphotransferase (PAP), or an adenosine 5?-triphosphate (ATP) supply/regeneration system including adenosine 5?-monophosphate (AMP), polyphosphate, polyphosphate-driven nucleoside 5?-diphosphate kinase (PNDK), and adenylate kinase (ADK) is employed instead of ATP.
Abstract: Disclosed herein are polypeptides, polynucleotides encoding, cells and organisms comprising novel DNA-binding domains, including TALE DNA-binding domains. Also disclosed are methods of using these novel DNA-binding domains for modulation of gene expression and/or genomic editing of endogenous cellular sequences.
Type:
Application
Filed:
October 28, 2013
Publication date:
May 15, 2014
Applicant:
Sangamo BioSciences, Inc.
Inventors:
Philip D. Gregory, Jeffrey C. Miller, David Paschon, Edward J. Rebar, Siyuan Tan, Fyodor Urnov, Lei Zhang
Abstract: Many fungal secondary metabolites are of industrial interest, such as antibiotics, while others are undesirable compounds such as mycotoxins. Overexpression of mtfA enhances production of fungal compounds with applications in the medical field, and overexpression or impaired mtfA expression decreases the production of compounds that negatively affect health/agriculture/economy such as mycotoxins.
Type:
Application
Filed:
November 2, 2013
Publication date:
May 15, 2014
Applicant:
Board of Trustees of Northern Illinois University
Inventors:
Ana M. Calvo-Byrd, Vellaisamy Ramamoorthy
Abstract: The present disclosure generally relates to microorganisms (e.g., non-naturally occurring microorganisms) that comprise one or more polynucleotides coding for enzymes in a pathway that catalyze a conversion of a carbon source (e.g., a fermentable carbon source) to butadiene and succinate and the use of such microorganisms for the production of butadiene and succinate.
Type:
Application
Filed:
October 2, 2013
Publication date:
May 15, 2014
Applicant:
Braskem S/A AP 09
Inventors:
Mateus Schreiner Garcez Lopes, Avram Michael Slovic
Abstract: The present invention relates to isolated polypeptides having bicarbonate transporter activity and isolated polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides, and methods of producing C4-dicarboxylic acids, such as malic acid.
Type:
Application
Filed:
January 21, 2014
Publication date:
May 15, 2014
Applicant:
Novozymes, Inc.
Inventors:
Amanda Fischer, Stephen Brown, Sheryl Luttringer
Abstract: Disclosed herein are polypeptides, polynucleotides encoding, cells and organisms comprising novel DNA-binding domains, including TALE DNA-binding domains. Also disclosed are methods of using these novel DNA-binding domains for modulation of gene expression and/or genomic editing of endogenous cellular sequences.
Type:
Application
Filed:
October 28, 2013
Publication date:
May 15, 2014
Applicant:
Sangamo BioSciences, Inc.
Inventors:
Philip D. Gregory, Jeffrey C. Miller, David Paschon, Edward J. Rebar, Siyuan Tan, Fyodor Urnov, Lei Zhang
Abstract: The present invention is related to a fungal serine protease enzyme, which comprises an amino acid sequence the mature Fa_RF7182 enzyme having an amino acid sequence of SEQ ID NO: 18. The serine protease is obtainable from Fusarium acuminatum, more preferably from the deposited strain CBS 124084. Also disclosed are nucleic acid sequences encoding said protease, such as plasmid pALK2530 comprising the nucleotide sequence SEQ ID NO:12 deposited in Escherichia coli RF7803 under accession number DSM 22208 and plasmid pALK2531 comprising the full-length gene SEQ ID NO: 13 deposited in E. coli RF7879 under accession number DSM 22209, as well as fungal hosts, such as Trichoderma. Said protease is useful as an enzyme preparation applicable in detergent compositions and for treating fibers, for treating wool, for treating hair, for treating leather, for treating food or feed, or for any applications involving modification, degradation or removal of proteinaceous material.
Type:
Application
Filed:
October 30, 2013
Publication date:
May 15, 2014
Applicant:
AB Enzymes Oy
Inventors:
Kari Juntunen, Leena Valtakari, Susanna Makinen, Jarno Kallio, Jari Vehmaanpera, Pentti Ojapalo, Marja Paloheimo, George Szakacs
Abstract: The present disclosure relates to methods of controlling the sulfide (S2˜) content in systems, such as oil and gas reservoirs and pipelines, by the use of chlorine oxyanions and microorganisms with (per)chlorate-reducing activity.
Type:
Application
Filed:
May 31, 2012
Publication date:
May 15, 2014
Applicant:
The Regents of the University of California
Abstract: The present invention is related to a fungal serine protease enzyme, which comprises an amino acid sequence of the mature Fe_RF6318 enzyme having an amino acid sequence of SEQ ID NO: 15. The serine protease is obtainable from Fusarium equiseti, more preferably from the deposited strain CBS 119568. Also disclosed are nucleic acid sequences encoding said protease, such as plasmid pALK2521 comprising the nucleotide sequence SEQ ID NO:9 deposited in E. coli RF7664 under accession number DSM 22171 and plasmid pALK2529 comprising the full-length gene SEQ ID NO: 10 deposited in E. coli RF7800 under accession number DSM 22172. Said protease is useful as an enzyme preparation applicable in detergent compositions and for treating fibers, for treating wool, for treating hair, for treating leather, for treating food or feed, or for any applications involving modification, degradation or removal of proteinaceous material.
Type:
Application
Filed:
October 29, 2013
Publication date:
May 15, 2014
Applicant:
AB Enzymes Oy
Inventors:
Kari Juntunen, Leena Valtakari, Susanna Makinen, Jarno Kallio, Jari Vehmaanpera, Pentti Ojapalo, Marja Paloheimo
Abstract: The present invention relates to engineered multivalent and multispecific binding proteins, methods of making, and specifically to their uses in the prevention, diagnosis, and/or treatment of disease.
Type:
Application
Filed:
October 17, 2013
Publication date:
May 15, 2014
Applicant:
ABBVIE INC.
Inventors:
Tariq Ghayur, Junjian Liu, Sharmila Manoj, Susan E. Brophy
Abstract: The present invention relates to isolated polypeptides having bicarbonate transporter activity and isolated polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides, and methods of producing C4-dicarboxylic acids, such as malic acid.
Type:
Grant
Filed:
February 28, 2012
Date of Patent:
May 13, 2014
Assignee:
Novozymes, Inc.
Inventors:
Amanda Fischer, Stephen Brown, Sheryl Luttringer
Abstract: Described are compositions and methods relating to variant filamentous fungi having altered growth characteristics. Such variants are well-suited for growth in submerged cultures, e.g., for the large-scale production of enzymes and other proteins for commercial applications.
Type:
Application
Filed:
April 20, 2012
Publication date:
May 8, 2014
Applicant:
DANISCO US INC.
Inventors:
Elizabeth A. Bodie, Robert James Pratt II
Abstract: A ?-glucosidase exhibits high activity in the presence of biomass and has high thermal stability compared to conventional enzymes. The ?-glucosidase includes substitutions and/or deletions of amino acids, at least three amino acids selected from the group consisting of Glu39, Asp169, Arg170, Arg220, Tyr227, and Glu330, of the parent ?-glucosidase with other amino acids and exhibits a decomposition activity.
Abstract: The invention provides materials and methods for promoting weight loss or preventing weight gain, and in the treatment of diabetes, metabolic syndrome and associated disorders. In particular, the invention provides novel glucagon analogue peptides effective in such methods. The peptides may mediate their effect by having increased selectivity for the GLP-1 receptor as compared to human glucagon.
Type:
Application
Filed:
January 7, 2014
Publication date:
May 8, 2014
Inventors:
Eddi MEIER, Ditte Riber, Marie Skovgaard, Bjarne Due Larsen, Jens Rosengren Daugaard
Abstract: It is disclosed herein that SPANX-B is uniquely expressed in a number of human tumors and that SPANX-B is an immunogenic antigen that is recognized by human T cells inducing helper CD4+ and cytolytic CD8+ T cell responses. Specific SPANX-B polypeptides and polynucleotides are disclosed that can be used to generate an immune response. In several embodiments, these polypeptides can be used for the treatment of a variety of cancers, including melanoma, colon carcinoma, ovarian cancer, breast cancer, myeloma, lung carcinoma and renal cancer.
Type:
Application
Filed:
January 14, 2014
Publication date:
May 8, 2014
Applicant:
The United States of America, as represented by the Secretary, Department of Health and Human Serv
Abstract: A novel transformation system in the field of filamentous fungal hosts for expressing and secreting heterologous proteins or polypeptides is described. The invention also covers a process for producing large amounts of polypeptide or protein in an economical manner. The system comprises a transformed or transfected fungal strain of the genus Chrysosporium, more particularly of Chrysosporium lucknowense and mutants or derivatives thereof. It also covers transformants containing Chrysosporium coding sequences, as well expression-regulating sequences of Chrysosporium genes. Also provided are novel fungal enzymes and their encoding sequences and expression-regulating sequences.
Type:
Application
Filed:
September 17, 2012
Publication date:
May 8, 2014
Inventors:
Mark Aaron Emalfarb, Richard Paul Burlingame, Philip Terry Olson, Arkady Panteleimonovich Sinitsyn, Martine Parriche, Jean Christophe Bousson, Christine Marie Pynnonen, Peter Jan Punt, Cornelia Maria Johanna Van Zeijl
Abstract: The invention features methods producing isoprene and a co-product, such as ethanol, 1,3-propanediol, or hydrogen from cultured cells. The invention also provides compositions that include these cultured cells. The invention provides compositions comprising isoprene and ethanol, isoprene and 1,3-propanediol, and isoprene and hydrogen. Additionally, the invention provides methods of co-producing isoprene and ethanol, isoprene and 1,3-propanediol, and isoprene and hydrogen by culturing cells under conditions suitable for co-production of isoprene and ethanol, isoprene and 1,3-propanediol, and isoprene and hydrogen.
Type:
Application
Filed:
January 6, 2014
Publication date:
May 8, 2014
Applicants:
The Goodyear Tire & Rubber Company, Danisco US Inc.
Inventors:
Gopal K. CHOTANI, Joseph C. MCAULIFFE, Caroline M. PERES, Karl J. SANFORD, Derek H. WELLS
Abstract: The present invention pertains to a method of expressing a protein of interest, preferably a heterologous protein, in preferably a plant. In a preferred embodiment said plant is a doubled haploid homozygous transgenic Nicotiana tabacum plant silenced for Ntp303. Furthermore, the invention relates to said plant with or without nucleic acid constructs according to the invention. Propagation, harvest and tissue material of said transgenic Nicotiana tabacum plant is also a part of the invention.
Type:
Application
Filed:
November 5, 2013
Publication date:
May 8, 2014
Applicant:
R1 B3 Holding B.V.
Inventors:
Marinus Maria Antonius Van Herpen, Jozef Maurinus Raymond Hulzink, Anton Felix Croes
Abstract: Methods to convert lignocellulosic biomass to fermentable sugars with enzymes that degrade the lignocellulosic material are provided, as well as novel combinations of enzymes, including those that provide a synergistic release of sugars from plant biomass.
Type:
Application
Filed:
November 5, 2012
Publication date:
May 8, 2014
Applicant:
DYADIC INTERNATIONAL, INC.
Inventors:
Marco A. Baez-Vasquez, Richard Burlingame, Jon K. Magnuson, Marion Bradford, Arkady Panteleimonovich Sinitsyn
Abstract: The invention provides materials and methods for promoting weight loss or preventing weight gain, and in the treatment of diabetes, metabolic syndrome and associated disorders. In particular, the invention provides novel glucagon analogue peptides effective in such methods. The peptides may mediate their effect by having increased selectivity for the GLP-1 receptor as compared to human glucagon.
Type:
Application
Filed:
January 7, 2014
Publication date:
May 8, 2014
Inventors:
Eddi MEIER, Ditte Riber, Marie Skovgaard, Bjarne D. Larsen, Jens R. Daugaard, Trine S. R. Neerup
Abstract: Described is a method for increasing the N-glycosylation site occupancy of a therapeutic glycoprotein produced in recombinant host cells modified as described herein and genetically engineered to express the glycoprotein compared to the N-glycosylation site occupancy of the therapeutic glycoprotein produced in a recombinant host cell not modified as described herein. In particular, the method provides recombinant host cells that overexpress a heterologous single-subunit oligosaccharyltransferase, which in particular embodiments is capable of functionally suppressing the lethal phenotype of a mutation of at least one essential protein of the yeast oligosaccharyltransferase (OTase) complex, for example, the Leishmania major STT3D protein, in the presence of expression of the host cell genes encoding the endogenous OTase complex.
Abstract: A recombinant filamentous fungal cell (e.g. Aspergillus) having one or more inactivated chromosomal genes is provided. The chromosomal genes in some embodiments correspond to derA, derB, htmA, mnn9, mnn10, ochA, dpp4, dpp5, pepAa, pepAb, pepAc, pepAd, pepF and combinations thereof. The recombinant fungal cells may include further inactivated chromosomal genes which correspond to pepA, pepB, pepC and pepD. The recombinant filamentous fungal cells may include a heterologous nucleic acid encoding a protein of interest. Also provided are methods of producing a protein of interest in said recombinant filamentous fungal cell.
Abstract: The present invention relates to an isolated polypeptide comprising (a) the amino acid sequence set forth in SEQ ID NO: 21; or (b) an amino acid sequence that is at least 95% identical to SEQ ID NO: 21, and to therapeutic treatments based thereon.
Abstract: The invention found that partial deletion of the glyceraldehyde-3-phosphate dehydrogenase (gpd) promoter can enhance gene expression (even heterologous gene expression) in basidiomycetous fungi. With the discovery of these gpd promoters, an expression system can be constructed for the expression of a heterologous gene in mushroom. Accordingly, the invention provides a truncated glyceraldehyde-3-phosphate dehydrogenase promoter and a construct comprising the promoter of the invention operably linked to a heterologous transcribable polynucleotide molecule and a mushroom comprising the construct.
Abstract: The present invention relates to antibodies and antigen-binding portions thereof that specifically bind to insulin-like growth factor I receptor (IGF-IR), which is preferably human IGF-IR. The invention also relates to human anti-IGF-IR antibodies, including chimeric, bispecific, derivatized, single chain antibodies or portions of fusion proteins. The invention also relates to isolated heavy and light chain immunoglobulin molecules derived from anti-IGF-IR antibodies and nucleic acid molecules encoding such molecules. The present invention also relates to methods of making anti-IGF-IR antibodies, pharmaceutical compositions comprising these antibodies and methods of using the antibodies and compositions thereof for diagnosis and treatment. The invention also provides gene therapy methods using nucleic acid molecules encoding the heavy and/or light immunoglobulin molecules that comprise the human anti-IGF-IR antibodies.
Type:
Application
Filed:
December 19, 2013
Publication date:
May 1, 2014
Applicants:
PFIZER INC., AMGEN FREMONT INC.
Inventors:
Bruce D. Cohen, Jean Beebe, Jr., Penelope E. Miller, James D. Moyer, Jose Ramon Corvalan, Michael Gallo