Aspergillus Patents (Class 435/254.3)
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Publication number: 20120156249Abstract: The invention provides an isolated genetically modified non-mammalian organism, wherein the activity of acyl-CoA: sterol acyltransferase/sterol O-acyltransferase (EC 2.3.1.26) and/or diacylglycerol acyltransferase/diacylglycerol O-acyltranferase (EC 2.3.1.20) and/or lecithin cholesterol acyl transferase/phospholipid: diacylglycerol acyltransferase (EC 2.3.1.158) and/or acyl CoA-wax alcohol acyltransferase (EC 2.3.1.75) is reduced or abolished in comparison with a corresponding wildtype organism, methods of use of such an organism, shuttle vehicles for making such an organism and methods for producing such an organism.Type: ApplicationFiled: August 4, 2010Publication date: June 21, 2012Applicant: ORGANOBALANCE GMBHInventors: Christine Lang, Andreas Raab
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Publication number: 20120157384Abstract: The present invention pertains to PF4 muteins which comprise a substitution at position 67, e.g. a L67H substitution, compared to the sequence of the wild-type PF4 protein. Such PF4 muteins exhibit an increased anti-angiogenie activity and a reduced affinity for proteoglycans compared to the wild-type PF4 protein.Type: ApplicationFiled: July 26, 2010Publication date: June 21, 2012Inventors: Andreas Bikfalvi, Herve Prats, Cathy Quemener, Alexandre Dubrac
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Publication number: 20120156740Abstract: A non-naturally occurring microbial organism includes a microbial organism having a 1,4-cyclohexanedimethanol pathway that includes at least one exogenous nucleic acid encoding a 1,4-cyclohexanedimethanol pathway enzyme expressed in a sufficient amount to produce 1,4-cyclohexanedimethanol. A method for producing 1,4-cyclohexanedimethanol includes culturing a non-naturally occurring microbial organism having a 1,4-cyclohexanedimethanol pathway. The pathway includes at least one exogenous nucleic acid encoding a 1,4-cyclohexanedimethanol pathway enzyme expressed in a sufficient amount to produce 1,4-cyclohexanedimethanol, under conditions and for a sufficient period of time to produce 1,4-cyclohexanedimethanol.Type: ApplicationFiled: December 15, 2011Publication date: June 21, 2012Applicant: Genomatica, Inc.Inventors: Priti PHARKYA, Mark J. Burk
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Publication number: 20120156159Abstract: An acetyl xylan esterase variant having perhydrolytic activity is provided for producing peroxycarboxylic acids from carboxylic acid esters and a source of peroxygen. More specifically, a Thermotoga maritima acetyl xylan esterase gene was modified using error-prone PCR and site-directed mutagenesis to create an enzyme catalyst characterized by an increase in specific activity. The variant acetyl xylan esterase may be used to produce peroxycarboxylic acids suitable for use in a variety of applications such as cleaning, disinfecting, sanitizing, bleaching, wood pulp processing, and paper pulp processing applications.Type: ApplicationFiled: December 16, 2011Publication date: June 21, 2012Inventors: Robert DiCosimo, Mark Scott Payne, John Edward Gavagan
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Publication number: 20120156754Abstract: The invention provides variants of a Streptomyces sp. CBH2 that have improved properties compared to the wild type enzyme and methods of using the variants in the hydrolysis of substrates comprising cellulose.Type: ApplicationFiled: August 31, 2010Publication date: June 21, 2012Applicant: CODEXIS, INC.Inventors: Ish K. Dhawan, Erika N. Segraves
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Publication number: 20120156157Abstract: An acetyl xylan esterase variant having perhydrolytic activity is provided for producing peroxycarboxylic acids from carboxylic acid esters and a source of peroxygen. More specifically, a Thermotoga maritima acetyl xylan esterase gene was modified using error-prone PCR and site-directed mutagenesis to create an enzyme catalyst characterized by an increase in specific activity. The variant acetyl xylan esterase may be used to produce peroxycarboxylic acids suitable for use in a variety of applications such as cleaning, disinfecting, sanitizing, bleaching, wood pulp processing, and paper pulp processing applications.Type: ApplicationFiled: December 16, 2011Publication date: June 21, 2012Inventors: Robert DiCosimo, Mark Scott Payne, John Edward Gavagan
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Publication number: 20120156737Abstract: The invention relates to a method for preparing alpha-ketopimelic acid, comprising converting alpha-ketoglutaric acid into alpha-ketoadipic acid and converting alpha-ketoadipic acid into alpha-ketopimelic acid, wherein at least one of these conversions is carried out using a heterologous biocatalyst. The invention further relates to a heterologous cell, comprising one or more heterologous nucleic acid sequences encoding one or more heterologous enzymes capable of catalysing at least one reaction step in the preparation of alpha-ketopimelic acid from alpha-ketoglutaric acid.Type: ApplicationFiled: March 11, 2010Publication date: June 21, 2012Applicant: DSM IP ASSETS B.V.Inventors: Petronella Catharina Raemakers-Franken, Martin Schurmann, Axel Christoph Trefzer, Stefaan Marie Andre De Wildeman
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Publication number: 20120151633Abstract: The present invention relates to isolated polypeptides having endoglucanase activity and isolated polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods for producing and using the polypeptides.Type: ApplicationFiled: February 17, 2012Publication date: June 14, 2012Applicants: NOVOZYMES, INC., NOVOZYMES A/SInventors: Søren Flensted Lassen, Paul Harris, Elena Vlasenko, Kristian Bertel Romer Morkeberg Krogh
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Publication number: 20120149624Abstract: The present invention relates to novel subtilase variants exhibiting alterations relative to the parent subtilase in one or more properties including: Wash performance, thermal stability, storage stability or catalytic activity. The variants of the invention are suitable for use in e.g. cleaning or detergent compositions, such as laundry detergent compositions and dish wash compositions, including automatic dish wash compositions.Type: ApplicationFiled: December 20, 2010Publication date: June 14, 2012Applicant: Novozymes A/SInventors: Henriette Draborg, Peter Kamp Hansen, Mads Eskelund Bjornvad, Mads Norregaard-Madsen, Mikael Mikkelsen
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Publication number: 20120149064Abstract: A filamentous fungal cell is provided comprising at least one mutation, wherein the filamentous fungal cell has impaired ptrB activity and has altered expression of a protein of interest as compared to a corresponding parent filamentous fungal cell. In one embodiment, the altered expression of the protein of interest is enhanced expression of the protein of interest.Type: ApplicationFiled: November 17, 2009Publication date: June 14, 2012Applicant: Danisco Us Inc.Inventors: Huaming Wang, Guomin Tang, Aoquan Wang, Jinxiang Zhang
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Publication number: 20120149078Abstract: The present invention relates to isolated polypeptides having acetylxylan esterase activity and isolated polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides.Type: ApplicationFiled: March 1, 2012Publication date: June 14, 2012Applicant: NOVOZYMES A/SInventors: Michelle Maranta, Kimberly Brown
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Publication number: 20120148706Abstract: By combination of hydrophobic chromatography and strongly basic anion-exchange chromatography, a novel, highly hydrophobic ?-glucosidase was successfully identified from Acremonium cellulolyticus. Further, a gene corresponding to the identified ?-glucosidase was isolated. When multiple modifications were introduced into the base sequence of the gene, the gene was successfully expressed in Trichoderma viride at a high level, and the expression product successfully exhibited a high ?-glucosidase activity.Type: ApplicationFiled: August 17, 2010Publication date: June 14, 2012Applicant: MEIJI SEIKA PHARMA CO., LTD.Inventors: Fumikazu Yokoyama, Kengo Yokoyama, Nobuko Mazuka
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Publication number: 20120148585Abstract: The invention concerns bifunctional fusion molecules, and novel, safer and more efficacious methods for the treatment of immune disorders resulting from excessive or unwanted immune responses. The invention provides methods for the suppression of type I hypersensitive (i.e., IgE-mediated) allergic conditions, methods for the prevention of anaphylactic responses that occur as a result of traditional peptide immunotherapies for allergic and autoimmune disorders, and provides novel methods for the treatment of autoimmune conditions, where the methods have reduced risk of triggering an anaphylactic response. The invention provides novel therapeutic approaches for the treatment of allergic responses, including the prevention of anaphylactic response that can occur from environmental allergen exposure. The invention also provides methods for the treatment of autoimmune disorders such as multiple sclerosis, autoimmune type I diabetes mellitus, and rheumatoid arthritis.Type: ApplicationFiled: December 17, 2010Publication date: June 14, 2012Inventor: Andrew Saxon
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Publication number: 20120149079Abstract: The present invention relates to isolated polypeptides having C4-dicarboxylic acid transporter activity and isolated polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides, and methods of producing C4-dicarboxylic acids, such as malic acid.Type: ApplicationFiled: February 24, 2012Publication date: June 14, 2012Applicant: NOVOZYMES, INC.Inventors: Amanda Fischer, Debbie Yaver
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Publication number: 20120148592Abstract: The present invention relates to novel humanized, chimeric and murine antibodies that have binding specificity for the human CC chemokine ligand 20 (CCL20). The present invention further relates to heavy chains and light chains of said antibodies. The invention also relates to isolated nucleic acids, recombinant vectors and host cells that comprise a sequence which encodes a heavy chain and/or a light chain of said antibodies, and to a method of preparing said antibodies. The anti-CCL20 antibodies of the invention can be used in therapeutic applications to treat, for example, inflammatory and autoimmune disorders and cancer.Type: ApplicationFiled: November 18, 2011Publication date: June 14, 2012Inventors: Toshio Imai, Brad Kline, Tetsu Kawano, Luigi Grasso, Yoshimasa Sakamoto, Jared Spidel, Miyuki Nishimura, Kenzo Muramoto, Tatsuo Horizoe
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Publication number: 20120151632Abstract: The present invention relates to phospholipase variants, polynucleotides encoding the variant and to nucleic acid constructs, vectors, and host cells comprising the polynucleotides, and methods of using the variant enzymes.Type: ApplicationFiled: June 8, 2010Publication date: June 14, 2012Applicant: NOVOZYMES A/SInventors: Leonardo De Maria, Jesper Vind, Morten Tovborg Jensen
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Publication number: 20120149087Abstract: The present disclosure relates to variants of a parent glucoamylase having altered properties (e.g., improved thermostability and/or specific activity). In particular, the present disclosure provides compositions comprising the variant glucoamylases, including starch hydrolyzing compositions and cleaning compositions. The disclosure also relates to DNA constructs encoding the variants and methods of producing the glucoamylase variants in host cells.Type: ApplicationFiled: September 30, 2011Publication date: June 14, 2012Applicant: Danisco US Inc.Inventors: Wolfgang Aehle, Richard R. Bott, Martijn Scheffers, Piet Van Solingen, Casper Vroemen
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Publication number: 20120141517Abstract: The present invention relates to peptides, nucleic acids and cells for use in immunotherapeutic methods. In particular, the present invention relates to the immunotherapy of cancer. The present invention furthermore relates to tumor-associated cytotoxic T cell (CTL) peptide epitopes, alone or in combination with other tumor-associated peptides that serve as active pharmaceutical ingredients of vaccine compositions that stimulate anti-tumor immune responses. The present invention relates to 30 peptide sequences and their variants derived from HLA class I and class II molecules of human tumor cells that can be used in vaccine compositions for eliciting anti-tumor immune responses.Type: ApplicationFiled: January 9, 2012Publication date: June 7, 2012Applicant: IMMATICS BIOTECHNOLOGIES GMBHInventors: Toni Weinschenk, Oliver Schoor, Claudia Trautwein, Norbert Hilf, Steffan Walter, Harpreet Singh
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Publication number: 20120141628Abstract: The invention relates to a method for the prevention or reduction of haze in a beverage by the addition of an prolyl-specific endoprotease and to new beverages obtainable by the method according to the invention. It also relates to new endoproteases. Sequence information of a genomic DNA, cDNA as well as protein sequences.Type: ApplicationFiled: January 13, 2012Publication date: June 7, 2012Applicant: DSM IP Assets B.V.Inventors: Michel LOPEZ, Luppo Edens
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Publication number: 20120141487Abstract: The present invention relates to isolated nucleic acid molecules which encode an antigen from a Moraxella catarrhalis (Meat) species, a vector which comprises such nucleic acid molecule, and a host cell comprising such vector. Furthermore, the invention provides antigens from a Moraxella catarrhalis species, as well as fragments and variants thereof, a process for producing such antigens, and a process for producing a cell which expresses such antigen. More specifically, such antigens are produced by or associated with bacterial infections caused by Moraxella catarrhalis.Type: ApplicationFiled: May 28, 2010Publication date: June 7, 2012Applicant: Intercell AGInventors: Alexander Von Gabain, Eszter Nagy, Andreas Meinke, Sanja Selak, Markus Hanner, Margarita Smidt, Julia Flor, Birgit Noiges, Wolfgang Schueler, Ulrike Schirmer, Verena Salletmayer, Mario Aistleithner, Stefan Seidel, Martin Oleksiewicz
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Publication number: 20120142051Abstract: Disclosed are: transcription regulatory factors capable of regulating the transcription or expression of genes for mannanases or cellulases, as mentioned below; and others. Specifically disclosed is a protein selected from the following proteins (a), (b) and (c): (a) a protein comprising the amino acid sequence depicted in SEQ ID NO:2; (b) a protein which comprises an amino acid sequence produced by deleting, substituting or adding one or several amino acid residues (e.g., 1 to 5 amino acid residues) in the amino acid sequence depicted in SEQ ID NO:2 and which is capable of regulating the transcription of genes for mannanases or cellulases; and (c) a protein which comprises an amino acid sequence having a 70% or higher sequence identity to the amino acid sequence depicted in SEQ ID NO:2 and which is capable of regulating the transcription of genes for mannanases or cellulases, or a partial fragment of the protein. Also specifically disclosed are a gene encoding the protein, and others.Type: ApplicationFiled: March 2, 2010Publication date: June 7, 2012Applicant: Noda Institute for Science ResearchInventors: Masahiro Ogawa, Yasuji Koyama, Masayuki Machida
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Publication number: 20120135453Abstract: Provided herein are methods for the treatment or prevention of a fungal infection in a host comprising the administration to the host a therapeutically or prophylactically effective amount of a CCA1 inhibitor.Type: ApplicationFiled: May 26, 2011Publication date: May 31, 2012Applicant: UCB Pharma SAInventor: Katherine Ann Vousden
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Publication number: 20120134993Abstract: Disclosed are bispecific binding proteins comprising a antibody/soluble receptor bispecific binding protein that reduces the biological activity of both VEGF-A and FGF. The FGF binding moieties are generally soluble FGFR3 or FGFR2. An Fc polypeptide is fused to the C-terminus of the FGF binding moiety and VEGF-A binding moiety are polypeptides fused using peptide or polypeptide linker sequences, and can be expressed as single bispecific binding protein. The bispecific antibody/soluble receptor binding proteins can be used to treat cancers characterized by solid tumor growth as well as other diseases.Type: ApplicationFiled: March 26, 2010Publication date: May 31, 2012Inventors: Qi Pan, Carl W. Birks, Pallavur V. Sivakumar
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Publication number: 20120134972Abstract: The present invention provides isolated polypeptides comprising a fragment of the amino acid sequence of SEQ ID NO:1, or a variant, derivative or fusion thereof, which is capable of binding specifically to and lysing cells of Clostridium difficile, wherein the polypeptide exhibits greater lytic activity on cells of Clostridium difficile than the polypeptide of SEQ ID NO: 1. The invention further provides means for producing the same, methods for killing bacterial cells such as cells of Clostridium difficile, as well as methods for diagnosing, treating and preventing diseases and conditions associated with infection of the same.Type: ApplicationFiled: May 26, 2010Publication date: May 31, 2012Applicant: PLANT BIOSCIENCE LIMITEDInventors: Melinda Mayer, Arjan Narbad
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Publication number: 20120128723Abstract: The present invention provides the amino acid and nucleic acid sequences of heavy chain and light chain complementarity determining regions of a cancer specific antibody. In addition, the invention provides cancer specific antibodies and immunoconjugates comprising the cancer specific antibody attached to a toxin or label, and methods and uses thereof. The invention also relates to diagnostic methods and kits using the cancer specific antibodies of the invention. Further, the invention provides a novel cancer-associated antigen and its uses thereof.Type: ApplicationFiled: November 16, 2011Publication date: May 24, 2012Applicant: VIVENTIA BIOTECHNOLOGIES INC.Inventors: Francina C. CHAHAL, Joycelyn ENTWISTLE, Jeannick CIZEAU, Nicholas Ronald GLOVER, Glen Christopher MACDONALD
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Publication number: 20120128669Abstract: Amino acid sequences are provided that are directed against and/or that can specifically bind protein F of hRSV, as well as to compounds or constructs, and in particular proteins and polypeptides, that comprise or essentially consist of one or more such amino acid sequences. The amino acid sequences, polypeptides and therapeutic compounds and compositions provided by the invention show an improved stability, less immunogenicity and/or improved affinity and/or avidity for protein F of hRSV. The invention also relates to the uses of such amino acid sequences, polypeptides, compounds or constructs for prophylactic and/or therapeutic purposes.Type: ApplicationFiled: June 7, 2010Publication date: May 24, 2012Applicant: Ablynx N.V.Inventors: Erik Depla, Catelijne Stortelers, Stephanie Staelens
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Publication number: 20120129778Abstract: The disclosure provides an anti-TGFbetaRII immunoglobulin single variable domain. The disclosure also provides a polypeptide, ligand or pharmaceutical composition for treating a disease associated with TGFbeta signaling and a disease selected from the group of: tissue fibrosis, such as pulmonary fibrosis, including idiopathic pulmonary fibrosis, liver fibrosis, including cirrhosis and chronic hepatitis, rheumatoid arthritis, ocular disorders, or fibrosis of the skin, including keloid of skin, and kidney such as nephritis, kidney fibrosis and nephrosclerosis, and a vascular condition, such as restenosis.Type: ApplicationFiled: July 27, 2010Publication date: May 24, 2012Applicant: Glaxo Group LimitedInventors: Caroline J. Dimech, Adriaan Allart Stoop, Rudolf Maria De Wildt, Steve Holmes
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Publication number: 20120122191Abstract: The present invention relates to methods for producing a polypeptide, comprising: (a) cultivating a fungal host cell in a medium conducive for the production of the polypeptide, wherein the fungal host cell comprises a nucleic acid construct comprising a first nucleotide sequence encoding a signal peptide operably linked to a second nucleotide sequence encoding the polypeptide, wherein the first nucleotide sequence is foreign to the second nucleotide sequence and the 3? end of the first nucleotide sequence is immediately upstream of the initiator codon of the second nucleotide sequence. The present invention also relates to the isolated signal peptide sequences and to constructs, vectors, and fungal host cells comprising the signal peptide sequences operably linked to nucleotide sequences encoding polypeptides.Type: ApplicationFiled: January 13, 2012Publication date: May 17, 2012Applicant: NOVOZYMES, INC.Inventors: Suchindra Maiyuran, Ana Fidantsef, Howard Brody
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Publication number: 20120122157Abstract: The present invention relates to a method for producing a polypeptide comprising using a signal peptide, to nucleic acid constructs comprising a first nucleotide sequence encoding the signal peptide and a second nucleotide sequence encoding a polypeptide which is foreign to the first nucleotide sequence. Furthermore, it also relates to expression vectors and host cells comprising the nuclei acid construct.Type: ApplicationFiled: January 24, 2012Publication date: May 17, 2012Applicant: NOVOZYMES A/SInventors: Tomoko Matsui, Henriette Draborg, Steffen Danielsen
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Publication number: 20120122171Abstract: The invention provides a non-naturally occurring microbial biocatalyst including a microbial organism having a 4-hydroxybutanoic acid (4-HB) biosynthetic pathway having at least one exogenous nucleic acid encoding 4-hydroxybutanoate dehydrogenase, succinyl-CoA synthetase, CoA-dependent succinic semialdehyde dehydrogenase, or ?-ketoglutarate decarboxylase, wherein the exogenous nucleic acid is expressed in sufficient amounts to produce monomeric 4-hydroxybutanoic acid (4-HB).Type: ApplicationFiled: January 11, 2012Publication date: May 17, 2012Applicant: GENOMATICA, INC.Inventors: Mark J. Burk, Stephen J. Van Dien, Anthony P. Burgard, Wei Niu
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Publication number: 20120122184Abstract: A new strain Bacillus sp. P203 (Bacillus plakortiensis) is disclosed. Isolated mature functional polypeptide which is obtainable from the bacterium strain Bacillus sp. P203 deposited under accession number DSM 17419 are disclosed.Type: ApplicationFiled: January 26, 2012Publication date: May 17, 2012Applicant: NOVOZYMES A/SInventors: Preben Nielsen, Reinhard Wilting, Martin Borchert
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Publication number: 20120115203Abstract: A highly active L-isoleucine dioxygenase from Bacillus thuringiensis is provided. A method for manufacturing (2S,3R,4S)-4-hydroxy-L-isoleucine or a salt thereof by reacting L-isoleucine in an aqueous solvent in the presence of L-isoleucine dioxygenase and isolating (2S,3R,4S)-4-hydroxy-L-isoleucine is also provided.Type: ApplicationFiled: January 5, 2012Publication date: May 10, 2012Inventors: Tomohiro Kodera, Sergey Vasilievich Smirnov, Natalia Nikolaevna Samsonova, Veronika Aleksandrovna Kotliarova, Natalia Yurievna Rushkevich, Yury Ivanovich Kozlov, Sakayu Shimizu, Jun Ogawa, Makoto Hibi, Vitaly Grigorievich Paraskevov
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Publication number: 20120114659Abstract: Monoclonal antibodies that act as potentiators, stimulators and agonists of guanylyl cyclase receptors are disclosed.Type: ApplicationFiled: November 17, 2009Publication date: May 10, 2012Applicants: MORPHOSYS AG, BOEHRINGER INGELHEIM PHARMACEUTICALS INC.Inventors: Alisa Waterman, Daniel Rajotte, Tobias Litzenburger, Alexandra Kraus
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Publication number: 20120114799Abstract: The invention relates to Citrobacter phytases derived from Citrobacter amalonaticus, Citrobacter gillenii, and related phytases. The phytases belong to the acid histidine phosphatase family, are acid-stable, and expectedly of a high specific activity. The invention also relates to the corresponding DNA, the recombinant and wild-type production of the phytases, as well as the use thereof, in particular in animal feed.Type: ApplicationFiled: November 3, 2011Publication date: May 10, 2012Applicant: Novozymes A/SInventors: Monica Takamiya Wik, Carsten Sjøholm
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Publication number: 20120107903Abstract: A mutant glucose dehydrogenase having an amino acid sequence at least 80% identical to SEQ ID NO:3 and having glucose dehydrogenase activity, wherein amino acid residues corresponding to positions 326, 365 and 472 of said amino acid sequence are replaced with glutamine, tyrosine and tyrosine, respectively, and wherein said mutant glucose dehydrogenase shows an improved substrate specificity to glucose and a reduced reactivity to disaccharides.Type: ApplicationFiled: October 26, 2011Publication date: May 3, 2012Applicants: BIOENGINEERING LABORATORIES, LLC, ARKRAY, Inc.Inventors: Koji Sode, Katsuhiro Kojima
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Publication number: 20120107905Abstract: The present invention relates to fungal serine protease variants, which comprise an amino acid substitution of valine at position 208 of the parent Fusarium equiseti Fe_RF6318 serine protease, wherein the position of the substitution corresponds to the amino acid sequence of the mature Fe_RF6318 enzyme defined in SEQ ID NO:2. The variants have improved thermal stability and/or detergent stability compared to the parent Fe_RF6318 enzyme. Preferably the substitution is V208I and more preferably the variants comprise additional amino acid changes which further increase the stability. Also disclosed are nucleic acid sequences encoding said protease variants as well as recombinant vectors and host cells for the production of the variants.Type: ApplicationFiled: October 28, 2011Publication date: May 3, 2012Applicant: AB ENZYMES OYInventors: Kari Juntunen, Leena Valtakari, Nina Hakulinen, Marja Paloheimo
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Publication number: 20120107873Abstract: The present invention relates to a method of producing a recombinant sweet protein in a filamentous fungus. The invention also relates to isolated polynucleotides encoding the sweet protein and to nucleic acid constructs, vectors, and host cells comprising the polynucleotides, as well as methods of using the sweet protein produced by the method of the invention.Type: ApplicationFiled: August 5, 2010Publication date: May 3, 2012Applicant: NOVOZYMES A/SInventors: Jesper Vind, Jeppe Wegener Tams, Lars Beier, Carsten Lillelund Olsen
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Publication number: 20120100250Abstract: The present invention relates to carbohydrate oxidases. The present invention also relates to polynucleotides encoding the variant carbohydrate oxidases and to nucleic acid constructs, vectors, and host cells comprising the polynucleotides, and methods of using the variant enzymes, such as, in preparing dough and dough product compositions.Type: ApplicationFiled: July 9, 2010Publication date: April 26, 2012Applicant: NOVOZYMES A/SInventors: Lars Henrik Oestergaard, Leonardo De Maria
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Publication number: 20120100596Abstract: The present invention relates to isolated polypeptides having organophosphorous hydrolase activity and isolated polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides.Type: ApplicationFiled: May 6, 2010Publication date: April 26, 2012Applicant: NOVOZYMES A/SInventors: Steffen Danielsen, Lars Kobberoee Skov, Ricardo Leite, Vincent Laize, M Leonor Cancela Da Fonseca
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Publication number: 20120094329Abstract: The disclosure provides methods and materials for increasing the expression of a protein of interest such as an antibody by a cell. ABC50 expression or activity is increased which increases expression of the protein or antibody of interest. The disclosure also provides methods and materials for increasing the sensitivity of a cell to an endoplasmic reticulum stress agent such as Econozole by decreasing the level of ABC50.Type: ApplicationFiled: May 5, 2010Publication date: April 19, 2012Applicant: UNIVERSITY HEALTH NETWORKInventor: Stuart A. Berger
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Publication number: 20120093811Abstract: The invention relates to an isolated antibody that specifically binds vascular endothelial growth factor-D (VEGF-D) and to a humanized antibody that specifically binds VEGF-D.Type: ApplicationFiled: April 1, 2010Publication date: April 19, 2012Inventors: Jason W. Simmonds, Andrew J. Pow, Vincent Emil W. Batori, Irene Koukoulas, George Kopsidas
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Patent number: 8158395Abstract: The present invention relates to isolated polypeptides having C4-dicarboxylic acid transporter activity and isolated polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides, and methods of producing C4-dicarboxylic acids, such as malic acid.Type: GrantFiled: June 21, 2011Date of Patent: April 17, 2012Assignee: Novozymes, Inc.Inventors: Amanda Fischer, Debbie Yaver
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Publication number: 20120090054Abstract: The present invention relates to isolated polypeptides having endoglucanase activity and isolated polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods for producing and using the polypeptides.Type: ApplicationFiled: December 19, 2011Publication date: April 12, 2012Applicants: Novozymes, Inc., Novozymes A/SInventors: Paul Harris, Elena Valsenko, Elizabeth Zaretsky, Marcus Sakari Kauppinnen
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Publication number: 20120088277Abstract: The invention relates to a variant of a parent fungal glucoamylase, which exhibits improved thermal stability and/or increased specific activity using saccharide substrates.Type: ApplicationFiled: December 19, 2011Publication date: April 12, 2012Applicant: NOVOZYMES A/SInventors: Bjarne Roenfeldt Nielsen, Allan Svendsen, Henrik Pedersen, Jesper Vind, Hanne Vang Hendriksen, Torben Peter Frandsen
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Publication number: 20120082996Abstract: IL-21 variants nucleic acid sequences are provided that encode a peptide having deletions and zero to ten conservative amino acid substitutions in the region of amino acid residues 65-96 of SEQ ID. NO:2.Type: ApplicationFiled: September 19, 2011Publication date: April 5, 2012Inventors: Siv Annegrethe Hjorth, Kent Bondensgaard, Dennis Madsen
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Publication number: 20120082622Abstract: Isolated polynucleotides encoding Macaca fascicularis CCL17 (CynoCCL17), polypeptides obtainable from expression of these polynucleotides, recombinant cells, and methods of use are disclosed.Type: ApplicationFiled: September 26, 2011Publication date: April 5, 2012Inventors: Michael Naso, Mary Ryan, Sandra Santulli-Marotto, Bethany Swencki-Underwood
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Patent number: 8148155Abstract: The present invention relates to methods for increasing homologous recombination of a nucleic acid sequence introduced into a host cell, comprising: (a) introducing into a population of filamentous fungal host cells a first nucleic acid sequence encoding a recombination protein and a second nucleic acid sequence comprising one or more regions which are homologous with the genome of the filamentous fungal host cell, wherein (i) the recombination protein promotes the recombination of the one or more regions with the corresponding homologous region in the host's genome to incorporate the second nucleic acid sequence by homologous recombination, and (ii) the number of host cells comprising the incorporated second nucleic acid sequence in the population is increased at least 20% compared to the same population without the first nucleic acid sequence; (b) and isolating from the population a filamentous fungal cell comprising the incorporated second nucleic acid sequence.Type: GrantFiled: April 21, 2003Date of Patent: April 3, 2012Assignee: Novozymes, Inc.Inventors: Paul Harris, Howard Brody
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Publication number: 20120077731Abstract: The present invention relates to amino acid sequences that are directed against/and or that can specifically bind Interleukin-6 Receptor (IL-6R) with improved affinity and/or avidity, and/or that have an improved efficacy and/or potency, and which are capable of (partially, or preferably totally) blocking the IL-6/IL-6R interaction and/or inhibit signalization through IL-6, 1L-6R and/or the IL-6/IL-6R complex. The invention further relates to compounds or constructs, and in particular proteins and polypeptides, that comprise or essentially consist of one or more such amino acid sequences.Type: ApplicationFiled: April 12, 2010Publication date: March 29, 2012Applicant: Ablynx N.V.Inventors: Els Anna Alice Beirnaert, Cedric Jozef Neotere Ververken, Joost Alexander Kolkman, Maarten Van Roy
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Publication number: 20120071419Abstract: The present invention relates to a method for preparing a target-specific non-antibody protein, and more particularly, to a method for preparing a target-specific non-antibody protein comprising the steps of: selecting non-antibody proteins having a structural complementarity with the target site of a target protein in a non-antibody protein library; calculating a binding energy of the selected non-antibody protein and the target protein; selecting a non-antibody protein having a favorable binding energy among the selected non-antibody proteins; selecting amino acid residues having a high binding energy among the interfacial amino acid residues of the selected non-antibody protein and the target protein; and substituting the selected amino acid residues with the amino acid residues having a low binding energy.Type: ApplicationFiled: April 14, 2010Publication date: March 22, 2012Applicant: POSTECH ACADEMY-INDUSTRY FOUNDATIONInventors: Yoon Sup Choi, Jun Ho Chung, Ji Ho Yoo, Hyun Soo Cho, Su Min Yoon, Kyung Lock Kim, Sung Ho Ryu, Sang Uk Kim
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Publication number: 20120071394Abstract: The present invention provides a polypeptide having a biological activity of the Chemotaxis Inhibitory Protein of Staphylococcus aureus (‘CHIPS’), the polypeptide comprising or consisting of the amino acid sequence of SEQ ID NO: 2, or a fragment or variant thereof having a biological activity of CHIPS, wherein the fragment or variant retains amino acid substitutions K40E, D42V, N77H, K100R, K105R, N111 K and/or G112A relative to the wildtype CHIPS protein of SEQ ID NO:1. In one embodiment, polypeptide consists of the amino acid sequence of SEQ ID NO: 2. Related aspects of the invention provide pharmaceutical compositions comprising a polypeptide of the invention, together with methods or making and using the same.Type: ApplicationFiled: November 30, 2009Publication date: March 22, 2012Inventors: Christina Furebring, Anna Rosén, Karin Haraldsson, Erika Gustafsson, Björn Ulrik Walse