Aspergillus Patents (Class 435/254.3)
-
Publication number: 20130065297Abstract: An isolated mutated green fluorescent protein (gfp) gene for chromosomal insertion into a bacterium, wherein the gene is capable of being expressed in bacteria and produce sufficient fluorescence under illumination from a UV lamp in a bacterial colony to be seen by the naked eye. A gene cassette for inserting a gene into a chromosome.Type: ApplicationFiled: August 3, 2012Publication date: March 14, 2013Inventors: Leonardo B. Pinheiro, Peter Bergquist, Moreland Gibbs, Graham Vesey
-
Publication number: 20130065279Abstract: The invention provides a non-naturally occurring microbial organism having a methacrylic acid, methacrylate ester, 3-hydroxyisobutyrate and/or 2-hydroxyisobutyrate pathway. The microbial organism contains at least one exogenous nucleic acid encoding an enzyme in a methacrylic acid pathway. The invention additionally provides a method for producing methacrylic acid, methacrylate ester, 3-hydroxyisobutyrate and/or 2-hydroxyisobutyrate. The method can include culturing methacrylic acid, methacrylate ester, 3-hydroxyisobutyrate and/or 2-hydroxyisobutyrate producing microbial organism, where the microbial organism expresses at least one exogenous nucleic acid encoding a methacrylic acid pathway enzyme in a sufficient amount to produce methacrylic acid, methacrylate ester, 3-hydroxyisobutyrate and/or 2-hydroxyisobutyrate, under conditions and for a sufficient period of time to produce methacrylic acid, methacrylate ester, 3-hydroxyisobutyrate and/or 2-hydroxyisobutyrate.Type: ApplicationFiled: March 30, 2012Publication date: March 14, 2013Applicant: Genomatica, Inc.Inventors: Mark J. BURK, Anthony P. Burgard, Robin E. Osterhout, Jun Sun, Priti Pharkya
-
Publication number: 20130066035Abstract: Provided herein are non-naturally occurring eukaryotic organisms that can be engineered to produce and increase the availability of cytosolic acetyl-CoA. Also provided herein are non-naturally occurring eukaryotic organisms having a 1,3-butanediol (1,3-BDO) pathway. and methods of using such organisms to produce 1,3-BDO.Type: ApplicationFiled: September 7, 2012Publication date: March 14, 2013Applicant: Genomatica, Inc.Inventors: Anthony P. Burgard, Mark J. Burk, Robin E. Osterhout, Priti Pharkya, Jingyi Li
-
Publication number: 20130065261Abstract: The purpose of the present invention is to provide an FAD-conjugated glucose dehydrogenase that is hard to be inhibited by the inhibitors such as 1,10-phenanthroline. The present invention relates to a modified glucose dehydrogenase (GLD), comprising an amino acid sequence of a wild-type FAD-conjugated glucose dehydrogenase (GLD) represented by SEQ ID NO: 1 having a substitution of at least one amino acid residue selected from the group consisting of amino acid residues at positions 298, 338, 340, 341, 343, 352, 354, 424, 426, 431 and 432, wherein the modified GLD has a reduced susceptibility to an inhibitor, as compared with the wild-type GLD, especially to said modified GLD, which has 40% or more of a relative activity when determined in a system wherein the inhibitor coexists at a final concentration of 1 mM based on an enzymatic activity when determined in a system wherein the inhibitor does not coexist.Type: ApplicationFiled: August 30, 2012Publication date: March 14, 2013Inventors: Michinari HONDA, Tsuyoshi Kameda, Fuminao Kobayashi
-
Publication number: 20130059034Abstract: The present invention relates to polypeptides, specifically polypeptides having transgalactosylating activity and nucleic acids encoding these, and their uses in e.g. dairy product.Type: ApplicationFiled: March 29, 2011Publication date: March 7, 2013Applicant: DUPONT NUTRITION BIOSCIENCES APSInventors: Morten Krog Larsen, Charlotte Horsmans Poulsen
-
Publication number: 20130059353Abstract: Provided herein are host cells comprising carbonic anhydrase activity, wherein the cells are capable of producing C4-dicarboxylic acid. Also provided are methods of producing C4-dicarboxylic acid comprising (a) cultivating the host cells having carbonic anhydrase activity in a medium under suitable conditions to produce C4-dicarboxylic acid; and (b) recovering the C4-dicarboxylic acid.Type: ApplicationFiled: August 17, 2012Publication date: March 7, 2013Applicant: NOVOZYMES, INC.Inventors: Sarah McFarland, Stephen Brown, Sheryl Luttringer
-
Publication number: 20130059785Abstract: The present invention provides a polypeptide comprising (i) a leader sequence, the leader sequence comprising a (a) secretion pre sequence, and (b) the following motif: -X1-X2-X3-X4-X5- where X1 is phenylalanine, tryptophan, or tyrosine, X2 is isoleucine, leucine, valine, alanine or methionine, X3 is leucine, valine, alanine or methionine, X4 is serine or threonine and X5 is isoleucine, valine, alanine or methionine; and (ii) a desired protein heterologous to the leader sequence. A polypeptide of the invention may additionally comprise, as part of the leader sequence, a secretion pro sequence. The invention also provides a polynucleotide comprising a sequence that encodes a polypeptide of the invention and a cell, preferably a yeast cell, comprising said polynucleotide.Type: ApplicationFiled: August 8, 2012Publication date: March 7, 2013Applicant: NOVOZYMES BIOPHARMA DK A/SInventor: Darrell Sleep
-
Publication number: 20130058927Abstract: Humanized and variant anti-VEGF antibodies and various uses therefor are disclosed. The anti-VEGF antibodies have strong binding affinities for VEGF; inhibit VEGF-induced proliferation of endothelial cells in vitro; and inhibit tumor growth in vivo.Type: ApplicationFiled: October 31, 2012Publication date: March 7, 2013Applicant: GENENTECH, INC.Inventor: GENENTECH, INC.
-
Publication number: 20130061354Abstract: The invention relates to a polypeptide comprising the amino acid sequence set out in SEQ ID NO: 2 or an amino acid sequence encoded by the nucleotide sequence of SEQ ID NO: 1, or a variant polypeptide or variant polynucleotide thereof, wherein the variant polypeptide has at least 93% sequence identity with the sequence set out in SEQ ID NO: 2 or the variant polynucleotide encodes a polypeptide that has at least 93% sequence identity with the sequence set out in SEQ ID NO: 2. The invention features the full length coding sequence of the novel gene as well as the amino acid sequence of the full-length functional polypeptide and functional equivalents of the gene or the amino acid sequence. The invention also relates to methods for using the polypeptide in industrial processes. Also included in the invention are cells transformed with a polynucleotide according to the invention suitable for producing these proteins.Type: ApplicationFiled: February 11, 2011Publication date: March 7, 2013Applicant: DSM IP ASSETSInventors: Cornelis Maria Jacobus Sagt, Margot Elisabeth Francoise Schoonneveld-Bergmans, Johannes Andries Roubos, Alrik Pieter Los
-
Patent number: 8389269Abstract: The present invention relates to a method to improve the secretion of a protein of interest by a filamentous fungal cell comprising inducing a phenotype in the cell selected from the group consisting of a lowered ERAD, an elevated UPR that does not induce an elevated ERAD, wherein ERAD preferably is lowered. The invention further relates to the filamentous fungal cell comprising the phenotype described above. The invention also relates to polynucleotides and polypeptides whose expression can be modulated in the filamentous fungal cell to obtain the above-described phenotype.Type: GrantFiled: October 31, 2007Date of Patent: March 5, 2013Assignee: DSM IP Assets B.V.Inventors: Cornelis Maria Jacobus Sagt, Cornelis Theodorus Verrips, Walraven Henry Muller, Noël Nicolaas Maria Elisabeth Van Peij
-
Publication number: 20130052694Abstract: Provided are isolated cellobiohydrolases comprising a modified Glycoside Hydrolase (GH) Family 7 catalytic domain, a GH Family 7 catalytic domain and a modified Family 1 carbohydrate binding module (CBM), or both a modified Family 7 catalytic domain and a modified Family 1 CBM. Such isolated cellobiohydrolases exhibit from 45% to about 99.9% amino acid sequence identity to amino acids 1-436 of SEQ ID NO: 1 or to amino acids 1-438 of SEQ ID NO: 2 and improved activity on process substrates. Also provided are genetic constructs and genetically modified microbes for expressing the isolated cellobiohydrolases, a process for producing the isolated cellobiohydrolases, cellulase enzyme mixtures comprising the isolated cellobiohydrolase and a process for hydrolysing a cellulosic substrate with such cellulase enzyme mixtures.Type: ApplicationFiled: August 24, 2012Publication date: February 28, 2013Applicant: Iogen Energy CorporationInventors: Jacqueline Montalibet, Loreta Gudynaite-Savitch, Christopher Hill, Christopher D. Hindle, James A. Lavigne, Nabil Masri, Fuad Tahna, John J. Tomashek
-
Host Cells and Methods for Producing 1-Deoxyxylulose 5-phosphate (DXP) and/or a DXP Derived Compound
Publication number: 20130052692Abstract: The present invention provides for a genetically modified host cell capable of producing 1-deoxyxylulose 5-phosphate or 1-deoxy-D-xylulose 5-phosphate (DXP) (12), and optionally one or more DXP derived compounds, comprising: (a) a mutant RibB, or functional variant thereof, capable of catalyzing xylulose 5-phosphate and/or ribulose 5-phosphate to DXP, or (b) a YajO, or functional variant thereof, and a XylB, or functional variant thereof.Type: ApplicationFiled: August 16, 2012Publication date: February 28, 2013Applicant: THE REGENTS OF THE UNIVERSITY OF CALIFORNIAInventors: James Kirby, Jeffrey L. Fortman, Minobu Nishimoto, Jay D. Keasling -
Publication number: 20130052679Abstract: A method for analyzing L-threonine contained in an specimen, which includes the steps of mixing a sample containing the specimen with an L-threonine dehydrogenase derived from Cupriavidus necator and a coenzyme NAD+ and analyzing the amount of NADH or 2-amino-3-oxobutyric acid after a predetermined period; an L-threonine dehydrogenase derived from Cupriavidus necator, which is a novel L-threonine dehydrogenase (TDH; EC 1.1.1.103) and can be utilized in the above-mentioned analysis method; a method for preparing a gene or the like to be used in the preparation of the enzyme, or a method for preparing the enzyme; an L-threonine analysis kit which includes (A) the L-threonine dehydrogenase and (B) a coenzyme NAD+; an enzyme preparation for use in the analysis of L-threonine, which includes the L-threonine dehydrogenase contained in a buffer solution; and an enzyme sensor utilizing the L-threonine dehydrogenase.Type: ApplicationFiled: September 4, 2012Publication date: February 28, 2013Inventors: Yasuhisa Asano, Techawaree Ueatrongchit
-
Publication number: 20130052686Abstract: The present invention relates to isolated polypeptides having alpha-L-arabinofuranosidase activity and isolated polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides.Type: ApplicationFiled: October 26, 2012Publication date: February 28, 2013Applicant: NOVOZYMES A/SInventors: Michelle Maranta, Kimberly Brown, James Langston
-
Publication number: 20130045323Abstract: The present invention relates to methods for increasing the protein, oil, and/or amino acid content of a plant. The methods involve the manipulation of the expression level of trehalose-6-phosphate synthase (TPS) homologs. Expression cassettes for achieving such gene expression manipulation and transgenic plant cells and plants comprising the constructs and cassettes are also provided. Methods of plant breeding using these plants have also been developed.Type: ApplicationFiled: August 16, 2012Publication date: February 21, 2013Applicant: BASF PLANT SCIENCE COMPANY GMBHInventor: Heiko Härtel
-
Publication number: 20130045504Abstract: The present invention provides systems for producing engineered oleaginous yeast or fungi that express carotenoidsType: ApplicationFiled: August 15, 2012Publication date: February 21, 2013Applicant: DSM IP ASSETS B.V.Inventors: Richard Bailey, Kevin T. Madden, Joshua Trueheart
-
Publication number: 20130045219Abstract: This invention provides binding proteins, including antibodies, antibody derivatives and antibody fragments, that specifically bind a CD154 (CD40L) protein. This invention also provides a chimeric, humanized or fully human antibody, antibody derivative or antibody fragment that specifically binds to an epitope to which a humanized Fab fragment comprising a variable heavy chain sequence according to SEQ ID NO: 1 and comprising a variable light chain sequence according to SEQ ID NO: 2 specifically binds. CD154 binding proteins of this invention may elicit reduced effector function relative to a second anti-CD154 antibody. CD154 binding proteins of this invention are useful in diagnostic and therapeutic methods, such as in the treatment and prevention of diseases including those that involve undesirable immune responses that are mediated by CD154-CD40 interactions.Type: ApplicationFiled: October 22, 2012Publication date: February 21, 2013Applicants: BIOGEN IDEC MA INC., UCB PHARMA S.A.Inventors: UCB Pharma S.A., Biogen Idec MA Inc.
-
Publication number: 20130040345Abstract: The invention relates to a host cell comprising at least four different heterologous polynucleotides chosen from the group of polynucleotides encoding cellulases, hemicellulases and pectinases, wherein the host cell is capable of producing the at least four different enzymes chosen from the group of cellulases, hemicellulases and pectinases, wherein the host cell is a filamentous fungus and is capable of secretion of the at least four different enzymes. This host cell can suitably be used for the production of an enzyme composition that can be used in a process for the saccharification of cellulosic material.Type: ApplicationFiled: February 11, 2011Publication date: February 14, 2013Applicant: DSM IP ASSETS B.V.Inventors: Alrik Pieter Los, Cornelis Maria Jacobus Sagt, Margot Elisabeth Francoise Schoonneveld-Bergmans, Robbertus Antonius Damveld
-
Publication number: 20130040371Abstract: Provided are: an NAS variant characterized by having sweetness independent of pH in the case of combination with a polypeptide NBS variant by causing substitution mutation or deletion mutation in one or several amino acids out of the amino acid residues constituting a polypeptide NAS; an NBS variant characterized by having sweetness independent of pH in the case of combination with a polypeptide NAS variant by causing substitution mutation or deletion mutation in one or several amino acids out of the amino acid residues constituting a polypeptide NBS; a dimer comprising a combination of the NAS variant and the NBS variant; a recombinant vector containing a gene encoding the NAS variant and a gene encoding the NBS variant; and a transformant containing the vector.Type: ApplicationFiled: July 9, 2012Publication date: February 14, 2013Inventors: Keiko ABE, Kenichiro Nakajima, Tomiko Asakura, Takumi Misaka, Sho Ishii
-
Publication number: 20130040346Abstract: The present invention relates to variants of a parent cellobiohydrolase. The present invention also relates to polynucleotides encoding the cellobiohydrolase variants; nucleic acid constructs, vectors, and host cells comprising the polynucleotides; and methods of using the cellobiohydrolase variants.Type: ApplicationFiled: March 29, 2011Publication date: February 14, 2013Applicant: NOVOZYMES, INC.Inventor: Mark Wogulis
-
Publication number: 20130034894Abstract: A highly active allene oxide synthase that can be used in the production of a plant growth regulating agent (KODA) is provided.Type: ApplicationFiled: March 30, 2011Publication date: February 7, 2013Inventors: Mineyuki Yokoyama, Sari Kamichi, Kazuteru Takagi
-
Publication number: 20130029381Abstract: The invention provides non-naturally occurring microbial organisms comprising a 1,4-butanediol (BDO), 4-hydroxybutyryl-CoA, 4-hydroxybutanal or putrescine pathway comprising at least one exogenous nucleic acid encoding a BDO, 4-hydroxybutyryl-CoA, 4-hydroxybutanal or putrescine pathway enzyme expressed in a sufficient amount to produce BDO, 4-hydroxybutyryl-CoA, 4-hydroxybutanal or putrescine and further optimized for expression of BDO. The invention additionally provides methods of using such microbial organisms to produce BDO, 4-hydroxybutyryl-CoA, 4-hydroxybutanal or putrescine.Type: ApplicationFiled: April 13, 2012Publication date: January 31, 2013Applicant: GENOMATICA, INC.Inventors: Robert Haselbeck, John D. Trawick, Wei Niu, Anthony P. Burgard
-
Publication number: 20130031676Abstract: The present invention relates to isolated polypeptides having glucoamylase activity and isolated polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides.Type: ApplicationFiled: April 11, 2011Publication date: January 31, 2013Applicant: Novozymes A/SInventors: Ming Li, Junxin Duan, Zheng Liu, Shiro Fukuyama, Keiichi Ayabe, Guillermo Coward-Kelly, Randall Deinhammer
-
Publication number: 20130023014Abstract: An object is to identify endoglucanase and ?-glucosidase genes by isolating genomic DNA containing cellulase genes, which are classified into endoglucanases or ?-glucosidases, from Acremonium cellulolyticus, and sequencing the nucleotide sequences thereof. The inventors intensively compared the amino acid sequences of known endoglucanases and ?-glucosidases with each other to find conserved region of amino acid sequences in Acremonium cellulolyticus, and various primers were designed based on the information. PCR was carried out using the various primers thus designed and genomic DNA or cDNA as a template. As a result, gene fragments of endoglucanases and ?-glucosidases were obtained. Primers were designed based on the gene fragments, and PCR was carried out to amplify nine genes of endoglucanases and ?-glucosidases. The nucleotide sequences thereof were sequenced, and the present invention was completed.Type: ApplicationFiled: March 31, 2010Publication date: January 24, 2013Applicant: MEIJI SEIKA PHARMA CO., LTD.Inventor: Fumikazu Yokoyama
-
Publication number: 20130025005Abstract: The present invention relates to isolated polypeptides having lipase activity and isolated polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods for producing and using the polypeptides.Type: ApplicationFiled: August 30, 2012Publication date: January 24, 2013Applicants: Novozymes A/S, Novozymes, Inc.Inventors: Debbie Yaver, Suzanne Otani, Janine Lin, Christopher Amolo, Kim Borch, Shamkant Anant Patkar, Michael Lamsa, Barbara Cherry
-
Publication number: 20130022607Abstract: The present invention relates to isolated antibodies and antigen-binding fragments that selectively bind human MAdCAM.Type: ApplicationFiled: August 31, 2012Publication date: January 24, 2013Inventor: Michael J. Briskin
-
Publication number: 20130023017Abstract: There is provided a method for culturing a microorganism in which a particular polynucleotide or a recombinant vector comprising it/them is introduced with an intermediate compound necessary for biosynthesis of pyripyropene. A. The method of the present invention allows for the production of pyripyropene.Type: ApplicationFiled: January 19, 2011Publication date: January 24, 2013Inventors: Hiroyuki Anzai, Kentaro Yamamoto, Kazuhiko Oyama, Mariko Tsuchida, Kimihiko Goto, Masaaki Mitomi
-
Publication number: 20130023028Abstract: The invention relates to a method of preparing a variant of a parent polypeptide comprising: (a) providing an amino acid sequence of a parent polypeptide; (b) substituting at least one amino acid residue at a position in the sequence corresponding to any of positions: 41, 83, 129, 207 or 284 in SEQ ID No: 2; (c) selecting a variant with lipolytic activity, which compared to the parent polypeptide has improved stability, and has an amino acid sequence with at least 60% identity to the mature polypeptide of SEQ ID No: 2; and (d) recovering the variant.Type: ApplicationFiled: December 2, 2010Publication date: January 24, 2013Applicants: Novozymes South Asia Pvt. Ltd., Novozymes A/SInventors: Allan Svendsen, Sangeeta Naik, Saikia Rakhi, Aditya Basu, Pritish Paul, Santhosh Maypadum Vasu, Leonardo De Maria, Michael Skjot
-
Publication number: 20130022539Abstract: The invention provides isolated anti-ovarian, pancreatic, lung or breast cancer antigen (Ovr115) antibodies that bind to Ovr115 on a mammalian cell in vivo. The invention also encompasses compositions comprising an anti-Ovr115 antibody and a carrier. These compositions can be provided in an article of manufacture or a kit. Another aspect of the invention is an isolated nucleic acid encoding an anti-Ovr115 antibody, as well as an expression vector comprising the isolated nucleic acid. Also provided are cells that produce the anti-Ovr115 antibodies. The invention encompasses a method of producing the anti-Ovr115 antibodies. Other aspects of the invention are a method of killing an Ovr115-expressing cancer cell, comprising contacting the cancer cell with an anti-Ovr115 antibody and a method of alleviating or treating an Ovr115-expressing cancer in a mammal, comprising administering a therapeutically effective amount of the anti-Ovr115 antibody to the mammal.Type: ApplicationFiled: June 14, 2012Publication date: January 24, 2013Applicant: diaDexus, Inc.Inventors: Glenn Pilkington, Gilbert-Andre Keller, Wenlu Li, Laura Corral, Iris Simon
-
Publication number: 20130018177Abstract: The present invention relates to eukaryotic host cells which have been modified to produce sialylated glycoproteins by the heterologous expression of a set of glycosyltransferases, including sialyltransferase and/or trans-sialidase, to become host-strains for the production of mammalian, e.g., human therapeutic glycoproteins. Novel eukaryotic host cells expressing a CMP-sialic acid biosynthetic pathway for the production of sialylated glycoproteins are also provided. The invention provides nucleic acid molecules and combinatorial libraries which can be used to successfully target and express mammalian enzymatic activities (such as those involved in sialylation) to intracellular compartments in a eukaryotic host cell. The process provides an engineered host cell which can be used to express and target any desirable gene(s) involved in glycosylation.Type: ApplicationFiled: July 20, 2012Publication date: January 17, 2013Applicant: GlycoFi, Inc.Inventor: Stephen R. Hamilton
-
Publication number: 20130017581Abstract: There is provided a nucleic acid construct comprising a pyripyropene biosynthetic gene cluster and a marker gene. The nucleic acid construct according to the present invention provides an inexpensive and highly productive method for producing pyripyropene.Type: ApplicationFiled: January 19, 2011Publication date: January 17, 2013Inventors: Sato Aihara, Naomi Sumida, Koichiro Murashima, Kouji Yanai, Hiroyuki Anzai, Kentaro Yamamoto
-
Publication number: 20130017224Abstract: Nucleic acid molecules comprising a nucleotide sequence encoding RANTES and fragments and variants thereof are disclosed. Additionally, nucleic acid molecules and compositions comprising the nucleotide sequence encoding RANTES and fragments and variants thereof in combination with nucleic acid sequences encoding immunogens are provided. Recombinant viral vectors comprising the nucleotide sequence encoding RANTES and fragments and variants thereof with or without a nucleic acid sequence encoding immunogens are also provided as are live attenuated pathogens comprising a nucleotide sequence encoding RANTES and fragments and variants thereof. Methods of modulating immune responses and of inducing an immune response against an immunogen are also disclosed.Type: ApplicationFiled: February 8, 2011Publication date: January 17, 2013Inventors: David B. Weiner, Jean D. Boyer, Michele Kutzler
-
Publication number: 20130017209Abstract: The invention relates to biparatopic A-beta binding polypeptides and, more specifically, to biparatopic A-beta binding polypeptides comprising at least two immunoglobulin single variable domains binding to different epitopes of A-beta. The invention also relates to specific sequences of such polypeptides, methods of their production, and methods of using them, including methods of treatment of diseases such as Alzheimer's Disease.Type: ApplicationFiled: September 14, 2012Publication date: January 17, 2013Applicant: BOEHRINGER INGELHEIM INTERNATIONAL GMBHInventors: John E. PARK, Cornelia DORNER-CIOSSEK, Stefan HOERER, Lothar KUSSMAUL, Martin LENTER, Katharina ZIMMERMANN, Gerald BESTE, Toon LAEREMANS, Pascal MERCHIERS, Jo VERCAMMEN
-
Publication number: 20130018174Abstract: The present invention provides efficient methods based on alteration of the protein A-binding ability, for producing or purifying multispecific antibodies having the activity of binding to two or more types of antigens to high purity through a protein A-based purification step alone. The methods of the present invention for producing or purifying multispecific antibodies which feature altering amino acid residues of antibody heavy chain constant region and/or variable region. Multispecific antibodies with an altered protein A-binding ability, which exhibit plasma retention comparable or longer than that of human IgG1, can be efficiently prepared in high purity by introducing amino acid alterations of the present invention into antibodies.Type: ApplicationFiled: December 24, 2010Publication date: January 17, 2013Applicant: CHUGAI SEIYAKU KABUSHIKI KAISHAInventors: Tomoyuki Igawa, Zenjiro Sampei, Tetsuya Wakabayashi, Eriko Ito
-
Publication number: 20130011876Abstract: The invention relates to a process for preparing filamentous fungal strains having a sexual cycle, wherein an acceptor filamentous fungal strain, having no or one type MAT locus, is subjected to recombination in which one or more mating type locus genes and optionally sex related genes, from other species than the acceptor filamentous fungal strain, is introduced into the acceptor filamentous fungal strain to produce a filamentous fungal strain having a sexual cycle.Type: ApplicationFiled: January 12, 2011Publication date: January 10, 2013Applicant: DSM IP ASSETTS, B.V.Inventors: Peter Jozef Ida Van De Vondervoort, Herman Jan Pel, Paul Stanley Dyer
-
Publication number: 20130011873Abstract: DNA and recombinant DNA that encode a peptide-forming enzyme, a method for producing a peptide-forming enzyme, and a method for producing a dipeptide are disclosed. A method for producing a dipeptide includes producing a dipeptide from a carboxy component and an amine component by using a culture of a microbe belonging to the genus Sphingobacterium and having the ability to form the dipeptide from the carboxy component and the amine component, a microbial cell separated from the culture, treated microbial cell product of the microbe or a peptide-forming enzyme derived from the microbe.Type: ApplicationFiled: September 12, 2012Publication date: January 10, 2013Applicant: Ajinomoto Co., Inc.Inventors: Seiichi HARA, Kenzo Yokozeki, Isao Abe, Naoto Tonouchi, Yasuko Jojima
-
Publication number: 20130011891Abstract: The invention provides non-naturally occurring microbial organisms having a butadiene or crotyl alcohol pathway. The invention additionally provides methods of using such organisms to produce butadiene or crotyl alcohol.Type: ApplicationFiled: June 19, 2012Publication date: January 10, 2013Inventors: Mark J. Burk, Anthony P. Burgard, Robin E. Osterhout, Jun Sun, Priti Pharkya
-
Publication number: 20130014293Abstract: The present invention relates to variants of a parent xylanase. The present invention also relates to polynucleotides encoding the variants; nucleic acid constructs, vectors, and host cells comprising the polynucleotides; and methods of using the variants.Type: ApplicationFiled: March 2, 2011Publication date: January 10, 2013Applicants: NOVOZYMES A/S, NOVOZYMES, INC.Inventors: Janine Lin, Jung Ye, Aubrey Jones, Suzanne Otani, Tia Heu, Pierre Cassland, Esben Friis
-
Patent number: 8349601Abstract: The present invention is related to glucoamylases having at least 80% sequence identity to a Trichoderma glucoamylase having the sequence of SEQ ID NO: 4 and biologically functional fragments thereof. The invention is also related to DNA sequences coding for the glucoamylases, vectors and host cells incorporating the DNA sequences, enzyme compositions and methods of using the glucoamylases in various applications.Type: GrantFiled: March 16, 2010Date of Patent: January 8, 2013Assignee: Danisco US Inc.Inventors: Nigel Dunn-Coleman, Paulien Neefe-Kruithof, Craig E. Pilgrim, Piet van Solingen, Donald E. Ward
-
Publication number: 20130004564Abstract: The present invention relates to novel peptides having antimicrobial activity, and compositions containing the same.Type: ApplicationFiled: December 13, 2010Publication date: January 3, 2013Applicants: UNIVERSITE DES SCIENCES ET TECHNOLOGIES DE LILLE, CENTRE NATIONAL DE LA RECHERCHE SCIENTIFIQUEInventors: Aurélle Tasiemski, Michel Salzet, Françoise Gaill
-
Publication number: 20130005012Abstract: In vivo methods for production of styrene via a recombinant host cell can include cell expression of at least one polynucleotide encoding a polypeptide having cinnamic acid decarboxylase activity, and at least one polynucleotide encoding a polypeptide having phenylalanine ammonia lyase activity.Type: ApplicationFiled: June 22, 2012Publication date: January 3, 2013Applicant: PHYTOGENE, INC.Inventor: Xiaodan Yu
-
Publication number: 20130005029Abstract: Described are variants (mutants) of a parent alpha-amylase having alpha-amylase activity and exhibiting altered properties relative to the parent alpha-amylase, and methods of use, thereof.Type: ApplicationFiled: July 2, 2012Publication date: January 3, 2013Applicant: Danisco US Inc., Genencor DivisionInventors: Luis G. CASCAO-PEREIRA, Claudine Chang, Clement Choy, James T. Kellis, JR., Brain E. Jones, Melodie Estabrook, Marc Kolkman, Chris Leeflang, Casper Vroemen, Walter Weyler
-
Patent number: 8343767Abstract: The present invention relates to methods for increasing homologous recombination of a nucleic acid sequence introduced into a host cell, comprising: (a) introducing into a population of filamentous fungal host cells a first nucleic acid sequence encoding a recombination protein and a second nucleic acid sequence comprising one or more regions which are homologous with the genome of the filamentous fungal host cell, wherein (i) the recombination protein promotes the recombination of the one or more regions with the corresponding homologous region in the host's genome to incorporate the second nucleic acid sequence by homologous recombination, and (ii) the number of host cells comprising the incorporated second nucleic acid sequence in the population is increased at least 20% compared to the same population without the first nucleic acid sequence; (b) and isolating from the population a filamentous fungal cell comprising the incorporated second nucleic acid sequence.Type: GrantFiled: March 29, 2012Date of Patent: January 1, 2013Assignee: Novozymes, Inc.Inventors: Paul Harris, Howard Brody
-
Publication number: 20120329126Abstract: The present invention provides DNA polymerases having increased efficiency of amplification of long amplicons. The present invention also provides for methods of amplifying target nucleic acid molecules with the DNA polymerases for increasing the efficiency of amplification of long amplicons.Type: ApplicationFiled: June 21, 2011Publication date: December 27, 2012Applicant: Bio-Rad Laboratories, Inc.Inventors: Yan Wang, John Sullivan
-
Publication number: 20120329119Abstract: The invention provides non-naturally occurring microbial organisms having a propylene pathway. The invention additionally provides methods of using such organisms to produce propylene.Type: ApplicationFiled: June 19, 2012Publication date: December 27, 2012Inventors: Anthony P. Burgard, Robin E. Osterhout, Jun Sun, Priti Pharkya, Mark J. Burk
-
Publication number: 20120328626Abstract: Described is a method for increasing the N-glycosylation site occupancy of a therapeutic glycoprotein produced in recombinant host cells modified as described herein and genetically engineered to express the glycoprotein compared to the N-glycosylation site occupancy of the therapeutic glycoprotein produced in a recombinant host cell not modified as described herein. In particular, the method provides recombinant host cells that overexpress a heterologous single-subunit oligosaccharyltransferase, which in particular embodiments is capable of functionally suppressing the lethal phenotype of a mutation of at least one essential protein of the yeast oligosaccharyltransferase (OTase) complex, for example, the Leishmania major STT3D protein, in the presence of expression of the host cell genes encoding the endogenous OTase complex.Type: ApplicationFiled: February 23, 2011Publication date: December 27, 2012Inventors: Natarajan Sethuraman, Byung-Kwon Choi, Bianka Prinz, Michael Meehl, Terrance Stadheim
-
Publication number: 20120331588Abstract: The present invention relates to isolated polypeptides having endoglucanase activity and isolated polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides.Type: ApplicationFiled: August 30, 2012Publication date: December 27, 2012Applicant: Novozymes A/SInventors: Alfredo Lopez de Leon, Michael Rey
-
Publication number: 20120329104Abstract: A modified microorganism having enhanced xylose utilization, an expression vector for constructing the modified microorganism, and a method of producing a chemical using the same are disclosed.Type: ApplicationFiled: June 27, 2012Publication date: December 27, 2012Applicants: SEOUL UNIVERSITY RESEARCH AND BUSINESS FOUNDATION, SAMSUNG ELECTRONICS CO., LTD.Inventors: Jae Young KIM, Hyun Min KOO, Young kyoung PARK, Jae Chan PARK, Sung Min PARK, Jin Ho SEO, Yong Cheol PARK
-
Publication number: 20120322148Abstract: This invention relates to the field of biotechnology or genetic engineering. Specifically, this invention relates to the field of gene expression. More specifically, this invention relates to a novel inducible gene expression system and methods of modulating gene expression in a host cell for applications such as gene therapy, large scale production of proteins and antibodies, cell-based high throughput screening assays, functional genomics and regulation of traits in transgenic plants and animals.Type: ApplicationFiled: May 3, 2012Publication date: December 20, 2012Applicant: Intrexon CorporationInventors: Subba Reddy Palli, Marianna Zinovjevna Kapitskaya, Dean Ervin Cress
-
Publication number: 20120321636Abstract: Compositions and methods relating to antibodies that specifically bind to TGF-beta binding proteins are provided. These methods and compositions relate to altering bone mineral density by interfering with the interaction between a TGF-beta binding protein sclerostin and a TGF-beta superfamily member, particularly a bone morphogenic protein. Increasing bone mineral density has uses in diseases and conditions in which low bone mineral density typifies the condition, such as osteopenia, osteoporosis, and bone fractures.Type: ApplicationFiled: August 22, 2012Publication date: December 20, 2012Applicant: UCB Manufacturing, Inc.Inventors: David G. Winkler, Jiye Shi, John Latham