Including Condition Or Time Responsive Control Means Patents (Class 435/286.1)
  • Patent number: 11312539
    Abstract: A bioreactor configured to contain a volume of liquid is provided. The bioreactor includes a collapsible bag able to contain the volume of liquid, a support structure surrounding and containing the collapsible bag, a first sparger connected to the collapsible bag, the first sparger having a first aperture size, and a second sparger connected to the collapsible bag, the second sparger having a second aperture size that is different from the first aperture size.
    Type: Grant
    Filed: July 2, 2020
    Date of Patent: April 26, 2022
    Inventors: Parrish M. Galliher, Geoffrey L. Hodge, Michael Fisher
  • Patent number: 11308121
    Abstract: A method and system for performing operations on cells on an instrument comprises providing a peer to peer network of instruments for performing operations on cells, maintaining a distributed database in at least a plurality of the instruments in the peer to peer network, storing at least one protocol in the database for use by at least one instrument in the peer to peer network for performing operations on cells and storing results from the performance of the operations on cells by the at least one instrument in the distributed database. The results stored in the distributed database is then usable to later authenticate the results from the at least one instrument.
    Type: Grant
    Filed: November 1, 2019
    Date of Patent: April 19, 2022
    Assignee: Thrive Bioscience, Inc.
    Inventors: Howard Cannon, Thomas Forest Farb
  • Patent number: 11274274
    Abstract: The invention discloses a bioreactor apparatus (1;101;201;301) for cultivation of cells comprising: a) a disposable bioreactor vessel (2) with one or more walls (3,4,5) defining an inner volume (6), at least one port (10) in a wall, wherein the disposable bioreactor vessel is positioned in a rigid support structure (8;108); and b) a heater (9;109;209;309), capable of heating an amount of culture medium to a target temperature in the range of 55-95° C., while the amount of culture medium is being confined in or conveyed to the inner volume.
    Type: Grant
    Filed: April 12, 2016
    Date of Patent: March 15, 2022
    Inventors: Colin R. Tuohey, Richard Lee Damren, Thomas John Erdenberger, Parish M. Galliher, Patrick M. Guertin
  • Patent number: 11253854
    Abstract: A microfluidic device may include a first fluid chamber, a second fluid chamber, a first microfluidic passage extending between the first fluid chamber and the second fluid chamber, a second microfluidic passage extending from the second fluid chamber, a first fluid actuator adjacent the first microfluidic passage and proximate the first fluid chamber to inertially pump fluid away from the first fluid chamber and a second fluid actuator adjacent the first microfluidic passage and proximate the second fluid chamber to menially pump fluid towards the first fluid chamber.
    Type: Grant
    Filed: October 20, 2017
    Date of Patent: February 22, 2022
    Assignee: Hewlett-Packard Development Company, L.P.
    Inventors: Pavel Kornilovich, Alexander N. Govyadinov
  • Patent number: 11230690
    Abstract: A data collection hub and method wherein a controller is configured to receive data from a plurality of sensors sensing conditions related to the performance of operations on cells by at least one instrument. A database stores the data from the plurality of sensors and the controller is configured to compare data from the plurality of sensors for a past operation with the data for a more recent operation.
    Type: Grant
    Filed: November 1, 2019
    Date of Patent: January 25, 2022
    Assignee: Thrive Bioscience, Inc.
    Inventors: Howard Cannon, Thomas Forest Farb
  • Patent number: 11118154
    Abstract: Aspects of the present invention relate to a networked cell culture incubator and to methods for operating such an incubator. In one aspect, the cell culture incubator includes a network interface for communicating with a source of parameter data utilized successfully by other incubators. The incubator receives appropriate parameter data and conducts the incubation process as prescribed by the parameter data so as to provide an improved environment for cell culture growth. The incubator may share its own parameter data with the data source for use by other incubators. The incubator and data source may share other forms of data as well.
    Type: Grant
    Filed: November 4, 2016
    Date of Patent: September 14, 2021
    Assignee: Thrive Bioscience, Inc.
    Inventor: Howard Cannon
  • Patent number: 11045566
    Abstract: A sterile packaging unit includes a disposable bioprocessing component (20) and a packaging (30). The disposable bioprocessing component (20) has a component wall that encloses a processing space and on which is secured a sensor system (40) with at least one sensor head (T, P, I, L, D) and with attached sensor electronics (42) that include a memory unit (44). The packaging (30) hermetically encloses the disposable bioprocessing component (20) and has a flexible packaging wall. The disposable bioprocessing component (20) and the packaging (30) are sterilized by being jointly irradiated with ionizing radiation. The sensor electronics (42) are connected electrically to a contact unit (34) extending through the packaging wall.
    Type: Grant
    Filed: September 22, 2016
    Date of Patent: June 29, 2021
    Assignee: Sartorius Stedim Biotech GmbH
    Inventors: Marco Leupold, Wei Gao, Stefan Schlack, Christian Grimm
  • Patent number: 10994277
    Abstract: An environmental control system for use in a clinical analyzer module includes an upper deck environmental subsystem comprising a far field sensor, one or more heaters, one or more spine cooling fans, and one or more in-line fluid heat exchangers. The far field sensor is configured to acquire measurements of ambient temperature in the upper deck environmental subsystem. The heaters are configured to generate hot airflow based on the measurements of ambient temperature from the far field sensor. The spine cooling fans are configured to operate in a manner that mixes the hot airflow from the heaters with cool airflow based on the measurements of ambient temperature from the far field sensor. The in-line fluid heat exchangers are configured to heat fluids used in reactions performed on the clinical analyzer module to a constant temperature.
    Type: Grant
    Filed: July 19, 2017
    Date of Patent: May 4, 2021
    Assignee: Siemens Healthcare Diagnostics Inc.
    Inventors: Michael Starr, Dimitri Shishkin, Shriram Patel, Beri Cohen, William Carpenter
  • Patent number: 10968422
    Abstract: A device for treating at least one biological cell includes at least one treatment support comprising a receiving surface enabling the adhesion of the at least one biological cell, at least one actuator capable of deforming said support in order to apply a stress to said at least one cell, and means of controlling said at least one actuator such that the actuator deforms the treatment support according to a given amplitude of deformation and for a given duration so as to generate at least one transitory pore in a membrane of the biological cell.
    Type: Grant
    Filed: October 27, 2016
    Date of Patent: April 6, 2021
    Assignees: Commissariat A L'Energie Atomique et aux Energies Alternatives, INSTITUT NATIONAL DE LA SANTE ET DE LA RECHERCHE MEDICALE (INSERM)
    Inventors: Fabrice Casset, Arnaud Millet
  • Patent number: 10955835
    Abstract: The present set of embodiments relate to an environmental condition monitoring and control system employing a software based virtual transmitter for a bioreactor or mixer. The system includes a probe for measuring a condition in a bioreactor that can generate a signal. Measured conditions may include pH, DO, temperature, and pressure. The signal can travel to an electronic card capable of converting the signal from analog to digital and the card can then send the converted signal to a digital controller. The condition monitoring and control system eliminates hardware redundancies and increases versatility by then displaying the converted signal in a software-based virtual transmitter on a non-dedicated human machine interface.
    Type: Grant
    Filed: December 13, 2018
    Date of Patent: March 23, 2021
    Assignee: Finesse Solutions, Inc.
    Inventors: Mark Selker, Barbara Paldus
  • Patent number: 10919042
    Abstract: A chemical and/or biochemical apparatus (10) for receiving a plurality of reaction vessels in which chemical and/or biochemical reactions may take place includes a thermal mount (14) having a plurality of wells (26) for receiving the reaction vessels (12), a thermal module (16) having a first side thermally coupled to the thermal mount (14), a first heat sink (18) thermally coupled to a second side of the thermal module, the heat sink (18) having a body and a plurality of thermally conductive fins (32) extending outwards from the body of the first heat sink (18), and a printed circuit board (54) having electronic components for controlling at least the thermal module (16), an excitation light source (62), and a light sensor (52). A first set of light waveguides (60) is provided for delivering excitation light to a reaction vessel, and a second set of light waveguides (38) is provided for receiving light from a reaction vessel and for delivering the light to the light sensor (52).
    Type: Grant
    Filed: April 7, 2015
    Date of Patent: February 16, 2021
    Assignee: IT-IS International Limited
    Inventors: James Richard Howell, Benjamin Masterman Webster
  • Patent number: 10829814
    Abstract: Presented are methods and compositions for obtaining sequence information from one or more individual cells. The methods are useful for obtaining sequence information for a single nucleotide sequence, and for multiplex generation of sequence information from one or more individual cells.
    Type: Grant
    Filed: June 17, 2015
    Date of Patent: November 10, 2020
    Assignees: Illumina, Inc., The Regents of the University of Caliofrnia
    Inventors: Jian-Bing Fan, Kun Zhang
  • Patent number: 10731919
    Abstract: A cooler includes a housing having a bottom surface, a front wall, a rear wall, two opposing sidewalls and an open top in communication with an interior storage chamber. A pivotal lid encloses the open top to provide selective access to the interior storage chamber. The lower surface of the lid includes a sensor that measures temperature, pressure and humidity within the interior chamber, and transmits the measured data to a portable electronic device to allow a remote user to monitor the condition of stored ice without opening the lid.
    Type: Grant
    Filed: January 4, 2016
    Date of Patent: August 4, 2020
    Inventors: John Gutierrez, Aamar Quershi
  • Patent number: 10704020
    Abstract: An incubator includes: an insulated box including an incubation space surrounded by multiple inner faces; a humidifying unit that humidifies an incubation space; a first heating unit that heats a first inner face among the multiple inner faces, with electric power supplied; a second heating unit that heats a second inner face, different from the first inner face, with electric power supplied; and a control unit that controls the magnitude of electric power supplied to each of the first heating unit and the second heating unit. The control unit repeatedly changes the magnitude of electric power supplied to the first heating unit at first timing, and also repeatedly changes the magnitude of electric power supplied to the second heating unit at second timing different from the first timing.
    Type: Grant
    Filed: March 13, 2019
    Date of Patent: July 7, 2020
    Inventors: Hironobu Sekine, Koichi Kobayashi
  • Patent number: 10698190
    Abstract: Optical systems and apparatuses configured for enabling substantially simultaneous observation of a plurality of points in an array from a common reference point. Without the optical systems and apparatuses disclosed herein, less than all of the plurality of points can be observed substantially simultaneously from the common reference point.
    Type: Grant
    Filed: March 14, 2018
    Date of Patent: June 30, 2020
    Assignee: BioFire Defense, LLC
    Inventors: Richard David Abbott, Patrick L. Riley, Zackery Kent Evans, Lyle M. Nay
  • Patent number: 10668469
    Abstract: A biological sorting apparatus is disclosed, which includes a light-induced dielectrophoretic chip, a supporting platform, an injecting unit and a projection module. The light-induced dielectrophoretic chip is configured to generate an internal electric field to perform sorting on a fluid including first microparticles and second microparticles. The supporting platform is utilized to support the light-induced dielectrophoretic chip thereon and has an opening. The injecting unit is configured to inject the fluid into the light-induced dielectrophoretic chip. The projection module is disposed below the supporting platform and is configured to project a light pattern onto a projection area of the light-induced dielectrophoretic chip through the opening of the supporting platform, such that the light-induced dielectrophoretic chip produces a light-induced effect to change the internal electric field, thereby sorting out the first microparticles and the second microparticles.
    Type: Grant
    Filed: April 19, 2017
    Date of Patent: June 2, 2020
    Inventors: Hung-Wei Wu, Cheng-Yuan Hung, Chang-Sin Ye
  • Patent number: 10642123
    Abstract: According to one embodiment, a method includes receiving light on a photoconductive layer of an electrophoretic deposition (EPD) device, the EPD device having a chamber defined by a first sheet, a second sheet and a spacer between the first and second sheets, where the first sheet is nonopaque and includes the photoconductive layer, where the second sheet is nonopaque and spaced from the first sheet, where a fluidic solution having a plurality of particles is in the chamber. The particles in the solution are attracted from suspension to illuminated portions of the photoconductive layer in the absence of an external voltage applied to the first and second sheets. The particles become deposited on the illuminated portions of the photoconductive layer.
    Type: Grant
    Filed: June 24, 2019
    Date of Patent: May 5, 2020
    Assignee: Lawrence Livermore National Security, LLC
    Inventors: Jeronimo Mora, Brian Giera, Jessica Katheryn Dudoff, Elaine Lee
  • Patent number: 10633624
    Abstract: Systems, methods, and apparatuses of controlling fluid flow are disclosed. An apparatus includes a first microplate having a first open portion and defining one or more first wells therein, a second microplate having a second open portion and defining one or more second wells therein, and a pneumatic lid constructed of styrene ethylene butylene styrene (SEBS). The pneumatic lid extends over the first open portion and the second open portion and includes one or more microfluidic channels that fluidly couple the one or more first wells to the one or more second wells. The pneumatic lid provides an airtight seal over the first microplate and the second microplate.
    Type: Grant
    Filed: July 1, 2019
    Date of Patent: April 28, 2020
    Inventors: Kirk S. Schroeder, Bradley D. Neagle, Eric Endsley, Daniel Appledorn, Keith Morton
  • Patent number: 10627337
    Abstract: A cell survival rate determining device includes a distribution acquisition unit acquiring a particle size distribution in a solution containing particles including cultured cells; a classification unit classifying the particles into a small particle group and a large particle group using a predetermined threshold in the distribution; a ratio calculation unit calculating the value of a ratio between the number of particles belonging to the small particle group and the number of particles belonging to the large particle group; and a survival rate determination unit determining the survival rate of the cultured cells from the value of the ratio using a pre-acquired relationship between the ratio and cell survival rate.
    Type: Grant
    Filed: April 18, 2018
    Date of Patent: April 21, 2020
    Inventor: Norio Hasegawa
  • Patent number: 10604733
    Abstract: An optofluidic photoreactor including an optical waveguide having an input, characterized by an evanescent optical field confined along an outer surface of the optical waveguide produced by radiation propagating in the optical waveguide, means for inputting light to the input of the optical waveguide, and a photoactive material disposed substantially only within the evanescent field. A method for optically activating a photoactive material in an optofluidic photoreactor to convert carbon dioxide and water into other molecules that may be useful as a fuel or a chemical feedstock.
    Type: Grant
    Filed: November 15, 2016
    Date of Patent: March 31, 2020
    Inventors: David Erickson, Perry M. Schein
  • Patent number: 10501719
    Abstract: Methods, apparatus, systems and articles of manufacture are disclosed herein to implement flexible bioreactor control systems. An example apparatus disclosed herein includes a processor coupled to a memory, the processor programmed to determine whether the map value included in the process task object is a valid map value, the process task object to correspond to a task executed by a bioreactor, a control device or a measurement device of the bioreactor control system configuration, in response to determining the map value is a valid map value, decode the map value to identify the source location of a first input of the process task object, pull a value from the source location to update the input value of the process task object, and facilitate execution of the process task with the input value.
    Type: Grant
    Filed: September 16, 2013
    Date of Patent: December 10, 2019
    Inventor: Scott A. Mangiacotti
  • Patent number: 10493419
    Abstract: Aspects of the present teachings describe a method and apparatus for automatically controlling a block temperature to reduce undershooting and overshooting of the temperatures of a sample contained in the block and participating in a polymerase chain reaction (PCR). The adaptive thermal block temperature control begins when a sample temperature enters a sample window region between a preliminary setpoint temperature and a target setpoint temperature for the sample. Based on thermodynamic behavior of the sample and the predetermined phase of PCR, predicting a time period measured subsequent to the preliminary setpoint temperature when the sample will reach the target setpoint suitable for the predetermined phase of PCR. During this time period, varying the block temperature ramp rate with a series of cooling and heating changes to ensure the block temperature reaches the target setpoint temperature at approximately the same time as the sample reaches the same.
    Type: Grant
    Filed: May 16, 2016
    Date of Patent: December 3, 2019
    Assignee: Applied Biosystems, LLC
    Inventors: Chee Kiong Lim, Chee Wee Ching
  • Patent number: 10076731
    Abstract: The present patent application describes a stirrer comprising a combination of at least one axially-conveying element and at least one radially-conveying element relative to the rotary shaft of the stirrer wherein the largest diameter of the at least one axially-conveying element is equal to or less than the inner diameter di of the radially-conveying element. In one embodiment the stirrer according to the invention is a combination of one anchor stirrer with at least one inclined-blade stirrer. Furthermore the use of the stirrer according to the invention for the culture of cells in a dialysis method is described.
    Type: Grant
    Filed: June 2, 2010
    Date of Patent: September 18, 2018
    Inventors: Marco Jenzsch, Max Lechner, Michael Pohlscheidt, Christoph Reese, Alexander Scholz, Hermann Tebbe
  • Patent number: 10052629
    Abstract: A hand-held molecular diagnostic test device includes a housing, an amplification (or PCR) module, and a detection module. The amplification module is configured to receive an input sample, and defines a reaction volume. The amplification module includes a heater such that the amplification module can perform a polymerase chain reaction (PCR) on the input sample. The detection module is configured to receive an output from the amplification module and a reagent formulated to produce a signal that indicates a presence of a target amplicon within the input sample. The amplification module and the detection module are integrated within the housing.
    Type: Grant
    Filed: May 4, 2017
    Date of Patent: August 21, 2018
    Assignee: Click Diagnostics, Inc.
    Inventors: Boris Andreyev, Keith E. Moravick, Brian Ciopyk, Victor Briones, Gregory Loney, Adam de la Zerda, Jesus Ching, Steven Chu, David Swenson, Helen Huang
  • Patent number: 10012640
    Abstract: A cell culture assay device can include: a substrate having a plurality of discrete microfluidic networks and a plurality of wells over the discrete microfluidic networks, each discrete microfluidic network having one or more wells fluidly coupled thereto, the wells extending upward from the discrete microfluidic networks; and a manifold body coupled with the substrate and having at least one fluid conduit pair for each microfluidic network and/or each well, each fluid conduit pair including a fluid inlet conduit and a fluid outlet conduit fluidly coupled to a corresponding microfluidic network and/or well. The substrate can be formed from a substrate base having the microfluidic networks coupled to a well plate having the wells associated with the microfluidic networks.
    Type: Grant
    Filed: February 14, 2014
    Date of Patent: July 3, 2018
    Inventors: Kapil Pant, Balabhaskar Prabhakarpandian
  • Patent number: 10005998
    Abstract: The invention relates to bioengineering and medical modular systems for the realization of self-contained equipment for influencing biological media, cells, tissue and tissue-like structures as objects. These modular systems distinguish themselves, in particular, by the fact that the user-specific equipment for influencing the objects can be realized in a simple and economically effective way. A tub-shaped main body for placement of elements that can be combined with one another is provided for this. Furthermore, at least one container for a liquid medium, a pumping system for a liquid medium, connection elements, filters for the inlet and outlet of gas, at least one filter with a connecting element for a medium, elements for a connection to at least one bioreactor, at least one gas inlet unit or a combination of at least one bioreactor and at least one gas inlet unit are components of the modular system.
    Type: Grant
    Filed: January 23, 2013
    Date of Patent: June 26, 2018
    Assignee: Alpha Plan GmbH
    Inventor: Hans Nagels
  • Patent number: 9994996
    Abstract: A raw-material supply device for supplying a biomass raw material under pressure, wherein the device is provided with: a screw body, provided inside a screw feeder and adapted for compressing, rotating, and transporting the powdered biomass raw material using a motor from a normal-pressure feed section for the fed biomass raw material to a high-pressure discharge section; and a pressing plug provided on the tip section side of the screw body, the pressing plug having a plug tip for maintaining a high-temperature/high-pressure field against the discharge force of compressed consolidated biomass.
    Type: Grant
    Filed: February 28, 2013
    Date of Patent: June 12, 2018
    Inventors: Minoru Genta, Seiichi Terakura, Ryosuke Uehara, Seiji Kobayashi
  • Patent number: 9921576
    Abstract: A bioreactor monitoring and/or control system utilizing only non-dedicated user input and information display devices, a digital controller and software, which system comprises: i) at least one diagnostic probe for measuring operating conditions in a bioreactor; ii) signal conditioning and communication electronics which supply operating current and/or voltage to the probe and which convert the diagnostic signal into a format accessible by a digital controller which directly receives the format converted signal and transmits it to software which enables the display device to show the converted signal, and also instruct the controller to implement changes in the operating conditions in the reactor, and iii) a software-based virtual transmitter which substantially replicates the keyboard, display, menu-tree and response of a physical sensor transmitter.
    Type: Grant
    Filed: May 5, 2008
    Date of Patent: March 20, 2018
    Assignee: Finesse Solutions, Inc.
    Inventors: Mark Selker, Barbara Paldus
  • Patent number: 9815056
    Abstract: Single-sided optoelectrowetting (SSOEW)-configured substrates are provided, as well as microfluidic devices that include such substrates. The substrates can include a planar electrode, a photoconductive (or photosensitive) layer, a dielectric layer (single-layer or composite), a mesh electrode, and a hydrophobic coating. Fluid droplets can be moved across the hydrophobic coating of such substrates in a light-actuated manner, upon the application of a suitable AC voltage potential across the substrate and the focusing of light into the photoconductive layer of the substrate in a location proximal to the droplets. Walls can be disposed upon the substrates to form the microfluidic devices. Together the walls and substrate can form a microfluidic circuit, through which droplets can be moved.
    Type: Grant
    Filed: December 4, 2015
    Date of Patent: November 14, 2017
    Inventors: Ming-Chiang Wu, Jodi Tsu-An Loo, Shao Ning Pei, Gaetan L. Mathieu, Jian Gong, Randall D. Lowe, Jr., Justin K. Valley
  • Patent number: 9643835
    Abstract: In one aspect, single-sided microfluidic devices are described herein. In some embodiments, a single-sided microfluidic device comprises a substrate, a photoconductive layer positioned over the substrate, electrical contacts in electrical communication with the photoconductive layer, and a dielectric assembly positioned over the photoconductive layer. The dielectric assembly comprises a hydrophobic surface for receiving a liquid. In some embodiments, the dielectric assembly has an effective capacitance of about 10 ?F/m2 to about 10,000 ?F/m2 and/or an average thickness between about 20 nm and about 2000 nm.
    Type: Grant
    Filed: September 3, 2013
    Date of Patent: May 9, 2017
    Assignee: University of North Carolina at Charlotte
    Inventors: Srinivas Akella, Vasanthsekar Shekar
  • Patent number: 9616426
    Abstract: A heater for heating fluidic elements and fluids is provided. The heater quickly and efficiently heats elements and samples without occupying a lot of space in in vitro diagnostic environments. The heater includes an induction coil, sized and configured to allow for a fluidic element to be placed therein, and induction circuitry coupled to the induction coil that facilitates induction heating through electromagnetic induction. A current is generated to pass through the induction coil, creating a field within the induction coil that generates heat that is transferrable to conductive objects placed within the field. In this manner, heat is transferred to the fluidic element and to fluids in contact with the fluidic element.
    Type: Grant
    Filed: August 23, 2013
    Date of Patent: April 11, 2017
    Assignee: Siemens Healthcare Diagnostics Inc.
    Inventor: Paul Dulaff
  • Patent number: 9505003
    Abstract: A portable real-time heating and detection device includes a body, a cover and a detection unit. The body has an opening, and a base. The cover has a control unit and a fix unit. The detection unit is disposed on the base of the body and has a thermostat, an optical excitation, an optical detection, and a circuit board. The thermostat is disposed close to the opening and has at least one thermostat zone. The optical exciter is disposed between the thermostat and the base. The optical detector is disposed between the thermostat and the opening. The circuit board is electrical coupled to the control unit, the thermostat, the optical excitation, and the optical detector, respectively.
    Type: Grant
    Filed: December 24, 2014
    Date of Patent: November 29, 2016
    Inventors: Chih-Hsiang Sung, Tseng-Huang Liu, Ruey-Shyan Hong, Ting-Hsuan Chen, Ping-Jung Wu, Kuo-Hsing Wen, Wan-Chi Chang
  • Patent number: 9348130
    Abstract: A device for holding filters for a microscope includes a filter wheel (20) rotatable about an axis of rotation (16) and a drive unit (14) for rotating the filter wheel (20). The filter wheel (20) comprises a basic body (44) rotatable about the axis of rotation (16) and at least one segment (46-54) selectively connectable to the basic body. The segment (46-54) comprises at least two housing areas (58, 59) each holding at least one filter (11). Another housing area may provide a transmission range (36, 59) for unfiltered transmission of light. A second rotatable filter wheel may be arranged at a location displaced along the axis of rotation relative to the filter wheel, and may have its own respective transmission range for unfiltered transmission of light, whereby a filter on either filter wheel may be aligned with the transmission range on the other filter wheel.
    Type: Grant
    Filed: June 13, 2014
    Date of Patent: May 24, 2016
    Assignee: Leica Microsystems CMS GmbH
    Inventors: Ingo Böhm, Dirk-Oliver Fehrer
  • Patent number: 9316586
    Abstract: This invention provides a system for performing PCR, and real time PCR in particular, with great speed and specificity. The system employs a heat block containing a liquid composition to rapidly transfer heat to and from reaction vessels. The system makes use of the reflective properties of the liquid metal to reflect signal from the PCR into the vessel and out the top. In this way, the signal can be measured by an optical assembly in real time without removing the vessels from the heat block.
    Type: Grant
    Filed: March 9, 2012
    Date of Patent: April 19, 2016
    Assignee: California Institute of Technology
    Inventors: George Maltezos, Matthew Johnston, David Goodwin, Axel Scherer, Christopher I. Walker
  • Patent number: 9303257
    Abstract: A cell cultivating platform includes a substrate having a surface, at least one actuator moveable relative to the substrate, and a deformable material layer positioned above at least a portion of the supporting surface and the at least one actuator. The deformable material layer positioned opposite the substrate surface includes a biocompatible supportive surface suitable for supporting cultivated cells. The supportive surface deforms or otherwise deflects in response to activation of the at least one actuator, effectively releasing at least a portion of the cultured cells from the supportive surface. Suitable actuators include piezoelectric actuators that can be selectively energized according to one or more patterns to facilitate separation of cells from the supportive surface. Such activation cycles can be repeated.
    Type: Grant
    Filed: July 1, 2010
    Date of Patent: April 5, 2016
    Inventors: Toshimi Fukui, Motoyuki Toki
  • Patent number: 9266104
    Abstract: The present invention provides thermocycling devices useful for amplification of nucleic acids in droplets. The thermocycling device utilizes the flow of one or more fluids through a main compartment at temperatures sufficient to conduct a polymerase chain reaction. Methods of amplifying nucleic acids in droplets are also provided.
    Type: Grant
    Filed: February 10, 2012
    Date of Patent: February 23, 2016
    Assignee: Raindance Technologies, Inc.
    Inventor: Darren Roy Link
  • Patent number: 9206383
    Abstract: Disclosed are bioreactor devices, systems and methods. A bioreactor system can include one or more bioreactor modules that can be individually controllable and identifiable. A bioreactor module can be connected to one or more functional modules such as a pump module, a stimulation signal generation module, a motor module, a mechanical transmission module, a gas exchange module, a temperature module, a humidity module and/or a CO2 module, among others. The bioreactor and functional modules can include standard or universal connectors to facilitate connection and movement of modules. The bioreactor system can be controlled and/or monitored by a controller that can individually identify and control each connected module and that can be adapted to collect signal data from sensors embedded in any of the modules.
    Type: Grant
    Filed: December 6, 2010
    Date of Patent: December 8, 2015
    Inventors: Gordana Vunjak-Novakovic, Warren Grayson, Qun Wan, Donald O. Freytes, Amandine Godier-Furnémont, Nina Tandon, Keith Yeager, George Eng, Sarindr Bhumiratana, Robert Maidhof
  • Patent number: 9090485
    Abstract: A core column simulator device and methods of assessing the efficacy of a wastewater biotreatment system using the core column simulator device are disclosed.
    Type: Grant
    Filed: October 12, 2010
    Date of Patent: July 28, 2015
    Inventor: Carl E. Adams
  • Patent number: 9060474
    Abstract: Systems and methods that facilitate the creation and harvesting of algae using tufted products. One exemplary tufted product comprises a substrate and tufts tufted through the substrate. The use of a tufted product provides various advantages with respect to the creation and harvesting of algae. Among other things, such products can be configured to improve the amount of algal-growing surface area provided and other growing environment characteristics and to facilitate the harvesting of the algae from the tufted product by facilitating the release of all or most of the algae from attachment to the tufted product.
    Type: Grant
    Filed: May 1, 2014
    Date of Patent: June 23, 2015
    Assignee: Interface, Inc.
    Inventors: William N. Jones, Daniel Price, James Hobbs
  • Patent number: 9045724
    Abstract: The present invention is directed to a photobioreactor for continuous or semi-continuous flow culturing photosynthesizing biomass and related method. In particular, the invention relates to a photobioreactor for continuous or semi-continuous flow culturing photosynthesizing algal biomass and related method. The photobioreactor comprises a gas feeding portion (60), a biomass-directing flow propulsion device (47) on a lighting system (5).
    Type: Grant
    Filed: June 6, 2011
    Date of Patent: June 2, 2015
    Inventor: Jean-Louis Roux Dit Buisson
  • Patent number: 9040272
    Abstract: The present invention provides a method and system for using eye-safe infrared energy from a Class I laser to manipulate cells in culture. The laser energy produces one or more phase boundary propulsion events, which generate hydrodynamic forces sufficient to manipulate cells at the focal point.
    Type: Grant
    Filed: June 13, 2011
    Date of Patent: May 26, 2015
    Assignee: Hamilton Thorne, Inc.
    Inventors: Diarmaid H. Douglas-Hamilton, Thomas G. Kenny
  • Publication number: 20150140545
    Abstract: An improved actuator for use in a microfluidic particle sorting system utilizes a staggered packing scheme for a plurality of actuators used to selectively deflect a particle in an associated sorting channel from a stream of channels. An actuator block may be provided for housing a two-dimensional array of actuators, each configured to align with an actuation port in an associated sorting chip containing a plurality of sorting channels. The actuator block may include a built-in stressing means to pre-stress each actuator housed by the block. An actuator comprising a piezo-electric stack may employ contact-based electrical connection rather than soldered wires to improve packing density. The actuator may be an external actuator. That is, the external actuator is external to the substrate in which the sorting channels are formed.
    Type: Application
    Filed: October 17, 2014
    Publication date: May 21, 2015
    Inventors: Andrew Johnson, John R. Gilbert, Manish Deshpande, Hugh Lewis, Bernard Bunner
  • Patent number: 9034639
    Abstract: A device is provided for use with a tissue penetrating system and/or a metering device for measuring analyte levels. The device comprises a cartridge and a plurality of analyte detecting members mounted on the cartridge. The cartridge may have a radial disc shape. The cartridge may also be sized to fit within the metering device. The analyte detecting members may be optical system using fluorescence lifetime to determine analyte levels. In one embodiment, the device may also include a fluid spreader positioned over at least a portion of the analyte detecting member to urge fluid toward one of the detecting members. A plurality of analyte detecting members may be used. Each analyte detecting member may be a low volume device.
    Type: Grant
    Filed: June 26, 2012
    Date of Patent: May 19, 2015
    Inventors: Dominique M. Freeman, Dirk Boccker, Robert Jones, David Cullen, Malcolm MacLood, William Carlson, Michael J. Owen, Christopher Dryer
  • Patent number: 9034635
    Abstract: A thermocycler apparatus and method for rapidly performing the PCR process employs at least two thermoelectric modules which are in substantial spatial opposition with an interior space present between opposing modules. One or multiple sample vessels are placed in between the modules such that the vessels are subjected to temperature cycling by the modules. The sample vessels have a minimal internal dimension that is substantially perpendicular to the modules that facilitates rapid temperature cycling. In embodiments of the invention the sample vessels may be deformable between: a) a shape having a wide mouth to facilitate filling and removing of sample fluids from the vessel, and b) a shape which is thinner for conforming to the sample cavity or interior space between the thermoelectric modules of the thermocycler for more rapid heat transfer.
    Type: Grant
    Filed: February 19, 2009
    Date of Patent: May 19, 2015
    Assignee: Streck, Inc.
    Inventors: Joel R. Termaat, Hendrik J. Viljoen, Scott E. Whitney
  • Patent number: 9029130
    Abstract: A contacting-type conductivity sensor includes an electrically-insulative plastic body and a plurality of electrodes. The plurality of conductive electrodes is disposed in the plastic body. Each electrode is constructed of plastic and fused with the electrically-insulative plastic body. A method of manufacturing the conductivity sensor is provided along with a single-use bioreactor employing the sensor.
    Type: Grant
    Filed: December 14, 2011
    Date of Patent: May 12, 2015
    Assignee: Rosemount Analytical Inc.
    Inventors: Chang-Dong Feng, Barry W. Benton
  • Publication number: 20150125937
    Abstract: A method and apparatus for managing a zone. Information is obtained about an environment in the zone with a sensor system. A determination is made by an analyzer system as to whether a contagious condition is present in the zone using the information from the sensor system. An action is performed by a management system in response to the contagious condition being present.
    Type: Application
    Filed: January 7, 2015
    Publication date: May 7, 2015
    Inventors: Leora Peltz, Shawn Hyunsoo Park
  • Publication number: 20150111196
    Abstract: A microfluidic chip orients and isolates components in a sample fluid mixture by two-step focusing, where sheath fluids compress the sample fluid mixture in a sample input channel in one direction, such that the sample fluid mixture becomes a narrower stream bounded by the sheath fluids, and by having the sheath fluids compress the sample fluid mixture in a second direction further downstream, such that the components are compressed and oriented in a selected direction to pass through an interrogation chamber in single file formation for identification and separation by various methods. The isolation mechanism utilizes external, stacked piezoelectric actuator assemblies disposed on a microfluidic chip holder, or piezoelectric actuator assemblies on-chip, so that the actuator assemblies are triggered by an electronic signal to actuate jet chambers on either side of the sample input channel, to jet selected components in the sample input channel into one of the output channels.
    Type: Application
    Filed: December 22, 2014
    Publication date: April 23, 2015
    Inventors: Zheng XIA, Yu ZHOU, John LARSEN, Guocheng SHAO, Shane PETERSON, Marjorie FAUST
  • Publication number: 20150111286
    Abstract: The present invention relates to a method and a system for reducing foam formation in slurry pits of swine rearing facilities with slatted floors.
    Type: Application
    Filed: November 21, 2013
    Publication date: April 23, 2015
    Applicant: Biocover A/S
    Inventor: Morten Toft
  • Publication number: 20150093740
    Abstract: A totipotent plant tissue multiplication system can include a sterile enclosure and a bioreactor in the sterile enclosure. The bioreactor can include a number of ports. A sensor can be inserted into a first port. A matter insertion system can input matter into the bioreactor via tubing configured to be connected to a second port. The sensor can be removed from the first port during operation of the matter insertion system. The tubing can be disconnected from the second port during operating of the sensor and the culture transfer system.
    Type: Application
    Filed: September 30, 2014
    Publication date: April 2, 2015
    Inventor: Anthony P. Swanda
  • Patent number: 8993308
    Abstract: A cryopreservation storage device (100), in particular for cryopreservation of biological samples, comprises a plurality of multi sample modules (20) being adapted for accommodating the biological samples and sample memories, a module control device (30) controlling an access to sample memories accommodated by the multi sample modules (20), and a data interface (41) for accessing to the module control device (30), wherein the module control device (30) includes a data management processor (31), which can be controlled via the data interface (41). Furthermore, a cryopreservation apparatus including at least one cryopreservation storage device (100) and a method for cryopreservation of biological samples are described.
    Type: Grant
    Filed: June 15, 2007
    Date of Patent: March 31, 2015
    Assignee: Fraunhofer-Gesellschaft zur Foerderung der angewandten Forschung e.V.
    Inventors: Heiko Zimmermann, Günter R. Fuhr, Stephen Shirley, Frank Ihmig