Including Means For Fluid Passage Between Compartments (e.g., Between Wells, Etc.) Patents (Class 435/288.5)
  • Patent number: 10941393
    Abstract: Microfluidic devices and methods for the encapsulation of particles within liquid droplets are disclosed. The new methods and devices form 1-100 picoliter-size monodisperse droplets containing the particles, such as single cells, encapsulated in individual liquid droplets. The particles can be encapsulated in droplets of a fluid by passing a fluid containing the particles through a high aspect-ratio microchannel to order the particles in the fluid, followed by forming the fluid into droplets. The resulting fraction of the liquid droplets with a single particle (e.g., a cell) is higher than the corresponding fraction of single-particle liquid droplets predicted by Poisson statistics.
    Type: Grant
    Filed: January 4, 2019
    Date of Patent: March 9, 2021
    Assignee: The General Hospital Corporation
    Inventors: Jon F. Edd, Mehmet Toner, Dino DiCarlo, Daniel Irimia
  • Patent number: 10919036
    Abstract: A flow cell is provided that includes surface-attached structures in a chamber. The structures are movable in response to a magnetic or electric field. A target extraction or isolation system includes the flow cell and a driver configured for applying a magnetic or electric field to the interior of the flow cell to actuate movement of the structures. The flow cell may be utilized to extract or isolate a target from a sample flowing through the flow cell. Further, a microfluidic system is provided that includes surface-attached structures and a microarray, wherein actuated motion of the surface-attached structures is used to enhance flow, circulation, and/or mixing action for analyte capture on the microarray.
    Type: Grant
    Filed: April 12, 2018
    Date of Patent: February 16, 2021
    Assignees: REDBUD LABS, INC., The University of North Carolina at Chapel Hill
    Inventors: Richard Chasen Spero, Jay Kenneth Fisher, Richard Superfine
  • Patent number: 10900886
    Abstract: The method, device, and system relate to particle analysis, and in particular, to a microfluidic device designed for trapping particles for analysis. Particles include beads and cells.
    Type: Grant
    Filed: April 20, 2018
    Date of Patent: January 26, 2021
    Assignee: EMD Millipore Corporation
    Inventors: Philip Janmin Lee, Paul Ju-Sung Hung, Narendra Maheshri
  • Patent number: 10900896
    Abstract: A flow cell is provided that includes surface-attached structures in a chamber. The structures are movable in response to a magnetic or electric field. A target extraction or isolation system includes the flow cell and a driver configured for applying a magnetic or electric field to the interior of the flow cell to actuate movement of the structures. The flow cell may be utilized to extract or isolate a target from a sample flowing through the flow cell. Further, a microfluidic system is provided that includes surface-attached structures and a microarray, wherein actuated motion of the surface-attached structures is used to enhance flow, circulation, and/or mixing action for analyte capture on the microarray.
    Type: Grant
    Filed: September 19, 2016
    Date of Patent: January 26, 2021
    Assignee: REDBUD LABS, INC.
    Inventors: Richard Chasen Spero, Jay Kenneth Fisher, Richard Superfine
  • Patent number: 10884008
    Abstract: Provided are: a device for storing biochemical reagents wherein an amount of a reagent can be hermetically stored and dropped from a storage site without coming into contact with the outside air; and a biochemical analyzer using the device. The device (10) is constituted by: sticking a top sheet (32) to a base sheet (31) provided with a convex-shaped hollow pocket (16) in which a reagent can be housed; a reagent container as a PTP packaging sheet (30) wherein an opening of the pocket in the base sheet (31), in which a reagent is preliminarily housed, is hermetically sealed with the top sheet (32); and sticking the film sheet surface as the top sheet (32) of the PTP packaging sheet (30), in which the reagent is hermetically packaged, to a cartridge surface of a device body (20) to thereby hermetically seal the inside of the device body too.
    Type: Grant
    Filed: April 27, 2015
    Date of Patent: January 5, 2021
    Assignee: HITACHI HIGH-TECH CORPORATION
    Inventors: Taro Nakazawa, Motohiro Yamazaki, Tatsuya Yamashita, Yoshitaka Kodama
  • Patent number: 10766029
    Abstract: Devices are for assessing the migration response in the presence of a stable encapsulated gradient of a factor or factor combination, and quantifying the adherence response inside micro-channels in the presence of different factors. A platform is for obtaining information relating to migration score or the quantification of adhered cells through use of the devices, and it allows this information to be used to assess therapeutic potential. A method quantifies the cells migration response and the cell adherence response.
    Type: Grant
    Filed: February 22, 2016
    Date of Patent: September 8, 2020
    Assignee: CELLS FOR CELLS S.A.
    Inventor: Juan Pablo Acevedo Cox
  • Patent number: 10761093
    Abstract: Disclosed is a system and method for a microdevice to separate blood cells based on differences in antigen expression. Specifically, cells of the same phenotype are separated based on whether or not they are activated during infection or resting. The device of the present disclosure takes a small sample of blood and provides differential cell counts that can be used to test for infection and inflammatory response. The device can be used to identify sepsis and other infections rapidly. By measuring differences in activated white cell counts such as neutrophils, the device of the present disclosure measures physiological response to infection in hospitalized patients recovering from burns, surgeries, etc.
    Type: Grant
    Filed: October 9, 2015
    Date of Patent: September 1, 2020
    Assignee: Texas Tech University System
    Inventor: Dimitri Pappas
  • Patent number: 10730041
    Abstract: Fluidic devices and related methods are generally provided. The fluidic devices described herein may be useful, for example, for diagnostic purposes (e.g., detection of the presence of one or more disease causing bacteria in a patient sample). Unlike certain existing fluidic devices for diagnostic purposes, the fluidic devices and methods described herein may be useful for detecting the presence of numerous disease causing bacteria in a patient sample substantially simultaneously (e.g., in parallel). In some embodiments, the fluidic devices and methods described herein provide highly sensitive detection of microbes in relatively large fluidic samples (e.g., between 0.5 mL and about 5 mL), as compared to certain existing fluidic detection (e.g., microfluidic) devices and methods. In an exemplary embodiment, increased detection sensitivity of microbial pathogens present in a patient sample (e.g., blood) is performed by selectively removing human nucleic acid prior to sensitive detection of microbial infection.
    Type: Grant
    Filed: March 14, 2017
    Date of Patent: August 4, 2020
    Assignee: HelixBind, Inc.
    Inventors: Alon Singer, Ranjit Prakash
  • Patent number: 10688492
    Abstract: Methods and systems are provided for isolating fetal cells from a maternal blood supply in order to perform non-invasive prenatal testing. In one example, a system for non-invasive prenatal testing includes a substrate coated with a cell-capturing surface, the cell-capturing surface including an array of pillar-like structures, each pillar-like structure including a plurality of intersecting arms.
    Type: Grant
    Filed: May 2, 2019
    Date of Patent: June 23, 2020
    Inventor: Lian Liu
  • Patent number: 10620108
    Abstract: Disclosed is a method of processing a specimen in which a target component in a specimen is processed using a specimen processing chip provided with a flow-path, the method including: introducing a fluid into a flow-path to form an interface that divides the fluid from a process liquid used for the processing of the target component with a rim of the interface on an inner wall of the flow-path, the process liquid containing particles including the target component; and moving the formed interface along the flow-path with the rim of the interface on the inner wall so as to force out the particles retained in the process liquid by the fluid.
    Type: Grant
    Filed: November 29, 2017
    Date of Patent: April 14, 2020
    Assignee: SYSMEX CORPORATION
    Inventors: Yasuko Kawamoto, Ayato Tagawa
  • Patent number: 10563164
    Abstract: Reader and plate methods, operations, and systems for observing a biological sample are shown and described. In one embodiment, a reader device includes selecting a plate type between at least two plate type selections on a user interface. One embodiment includes aligning a peel plate within a tray holder nest to define a semi-fixed processing position. One embodiment includes transporting a peel plate from a loading position into a focal alignment with an imaging device for monitoring biological growth, when present.
    Type: Grant
    Filed: October 11, 2016
    Date of Patent: February 18, 2020
    Assignee: Charm Sciences, Inc.
    Inventors: Richard T Skiffington, Robert S Salter, Robert J Markovsky, Stanley E Charm, Paul E Graham, Byron A. Roberts
  • Patent number: 10551382
    Abstract: The invention generally relates to methods for quantifying an amount of enzyme molecules. Systems and methods of the invention are provided for measuring an amount of target by forming a plurality of fluid partitions, a subset of which include the target, performing an enzyme-catalyzed reaction in the subset, and detecting the number of partitions in the subset. The amount of target can be determined based on the detected number.
    Type: Grant
    Filed: November 26, 2018
    Date of Patent: February 4, 2020
    Assignee: Bio-Rad Laboratories, Inc.
    Inventors: Darren Roy Link, Michael L. Samuels
  • Patent number: 10500167
    Abstract: The invention includes microfluidic methods and devices that allow for the continuous production of microfibers with embedded droplets aligned along the length of the fiber at specific positions. The invention allows for formation of single or multiple emulsions within a fiber. The various phases comprised within the fiber can vary in terms of in terms of hydrophobic/hydrophilic character, solid/fluid, or gel crosslink density, which allows for the introduction of heterogeneous microenvironments within the fiber, each of which with distinct solubility characteristics, permeability, and mechanical properties. Various compounds and materials can be encapsulated in the different microcompartments of the fiber for storage and delivery applications, as well as to provide multifunctionality to the fiber structure.
    Type: Grant
    Filed: December 6, 2017
    Date of Patent: December 10, 2019
    Assignee: THE TRUSTEES OF PRINCETON UNIVERSITY
    Inventors: Howard A. Stone, Janine K. Nunes, Eujin Um, Tamara Pico
  • Patent number: 10434515
    Abstract: A microfluidic device can include a temperature regulator positioned to generate a temperature gradient across a thermally controlled portion of the microfluidic device from a high temperature region to a low temperature region. Additionally, the microfluidic device can include a bi-directional microfluidic serpentine pathway having a first terminus and a second terminus. The bi-directional microfluidic serpentine pathway can be oriented along a longitudinal direction transverse to the temperature gradient. Further, the bi-directional microfluidic serpentine pathway can include a plurality of oscillation segments fluidly coupling the first terminus to the second terminus and forming a flow path adapted to oscillate a fluid between the high temperature region and the low temperature region. The bi-directional microfluidic serpentine pathway can have a uniform cross section to facilitate bi-directional flow.
    Type: Grant
    Filed: October 3, 2017
    Date of Patent: October 8, 2019
    Assignee: University of Utah Research Foundation
    Inventors: Raheel Samuel, Bruce Gale, Alex Jafek, James Trauba, Kenneth Aston
  • Patent number: 10345207
    Abstract: The method is for quantification of sub-visible particles. A filter membrane is provided that has a plurality of pores defined therethrough. The filter membrane is in operational engagement with a vacuum chamber. The pores are sealed with a sealant. A sample droplet, containing a liquid and sub-visible particles, is applied onto the filter membrane. The liquid dissolves the sealant in pores disposed directly below the sample droplet. The liquid flows through the pores in which the sealant has been dissolved and the sub-visible particles remain on top of the filter membrane. The particles are enumerated in an electron microscope.
    Type: Grant
    Filed: February 15, 2019
    Date of Patent: July 9, 2019
    Assignee: INTELLIGENT VIRUS INC.
    Inventors: Rickard Nordstrom, Ida-Maria Sintorn, Lars Haag
  • Patent number: 10247648
    Abstract: The method is for quantification of sub-visible particles. A filter membrane is provided that has a plurality of pores defined therethrough. The filter membrane is in operational engagement with a vacuum chamber. The pores are sealed with a sealant. A sample droplet, containing a liquid and sub-visible particles, is applied onto the filter membrane. The liquid dissolves the sealant in pores disposed directly below the sample droplet. The liquid flows through the pores in which the sealant has been dissolved and the sub-visible particles remain on top of the filter membrane. The particles are enumerated in an electron microscopy.
    Type: Grant
    Filed: October 21, 2016
    Date of Patent: April 2, 2019
    Assignee: Intelligent Virus Imaging Inc.
    Inventors: Rickard Nordstrom, Ida-Maria Sintorn, Lars Haag
  • Patent number: 10228317
    Abstract: Systems and methods for sorting particles are described. In one implementation, a system for sorting particles include a microfluidic device, a detection system, and an optical switching system. The microfluidic device includes a sample channel for passing through the particles in a fluid medium, a plurality of output channels fluidly connected to the sample channel at a plurality of junctions, and an actuation channel. The detection system detects a particle passing through the sample channel. The optical switching system deflects the particle to a target output channel based on results detected by the detection system. The optical switching system includes a modulation device configured to direct a laser pulse to an actuation region in the actuation channel corresponding to the target output channel. Advantageously, the systems and methods allow for accurate and dynamic sorting of a mixture of cells into a plurality of subpopulations using a simple microfluidic device.
    Type: Grant
    Filed: July 21, 2017
    Date of Patent: March 12, 2019
    Assignee: Verily Life Sciences LLC
    Inventors: Michael Brundage, Supriyo Sinha
  • Patent number: 10155207
    Abstract: The invention generally relates to methods for forming mixed droplets. In certain embodiments, methods of the invention involve forming a droplet, and contacting the droplet with a fluid stream, wherein a portion of the fluid stream integrates with the droplet to form a mixed droplet.
    Type: Grant
    Filed: June 2, 2016
    Date of Patent: December 18, 2018
    Assignee: Bio-Rad Laboratories, Inc.
    Inventors: Yevgeny Yurkovetsky, Darren R. Link, Jonathan William Larson
  • Patent number: 10139411
    Abstract: The invention generally relates to methods for quantifying an amount of enzyme molecules. Systems and methods of the invention are provided for measuring an amount of target by forming a plurality of fluid partitions, a subset of which include the target, performing an enzyme-catalyzed reaction in the subset, and detecting the number of partitions in the subset. The amount of target can be determined based on the detected number.
    Type: Grant
    Filed: January 25, 2017
    Date of Patent: November 27, 2018
    Assignee: Raindance Technologies, Inc.
    Inventors: Darren Roy Link, Michael L. Samuels
  • Patent number: 10118174
    Abstract: The present invention provides microfabricated substrates and methods of conducting reactions within these substrates. The reactions occur in plugs transported in the flow of a carrier-fluid.
    Type: Grant
    Filed: November 29, 2017
    Date of Patent: November 6, 2018
    Assignee: The University of Chicago
    Inventors: Rustem F. Ismagilov, Joshua David Tice, Helen Song Baca, Lewis Spencer Roach
  • Patent number: 10105702
    Abstract: Techniques are provided for generating, manipulating, and measuring fluidic droplets in mixed phase systems based on establishing transient continuities between otherwise spatially separated phases. In certain methods of the invention, electrodes in contact with the continuous phases allow electrical monitoring of continuity or proximity of separated phases as a means to characterize droplets. In other methods of the invention, fluidic continuity provides a means for generating droplets, injecting or extracting the contents of droplets, and sorting droplets. Chemical techniques are also provided that use these droplet-based methods, or others, to quantify and identify nucleic acids through incorporation into hydrogel particles. The nucleic acids are entrapped either actively by chemical incorporation during gel polymerization or passively by chain entanglement.
    Type: Grant
    Filed: March 17, 2014
    Date of Patent: October 23, 2018
    Assignee: Lariat Biosciences, Inc.
    Inventor: Jonathan W. Larson
  • Patent number: 10010882
    Abstract: A microfluidic device can comprise at least one swept region that is fluidically connected to unswept regions. The fluidic connections between the swept region and the unswept regions can enable diffusion but substantially no flow of media between the swept region and the unswept regions. The capability of biological micro-objects to produce an analyte of interest can be assayed in such a microfluidic device. Biological micro-objects in sample material loaded into a microfluidic device can be selected for particular characteristics and disposed into unswept regions. The sample material can then be flowed out of the swept region and an assay material flowed into the swept region. Flows of medium in the swept region do not substantially affect the biological micro-objects in the unswept regions, but any analyte of interest produced by a biological micro-object can diffuse from an unswept region into the swept region, where the analyte can react with the assay material to produce a localized detectable reaction.
    Type: Grant
    Filed: October 22, 2014
    Date of Patent: July 3, 2018
    Assignee: Berkeley Lights, Inc.
    Inventors: Mark P. White, Eric D. Hobbs, J. Tanner Nevill, Daniele Malleo, Steven W. Short
  • Patent number: 9968902
    Abstract: Certain aspects of the present invention provide devices and methods for preparing oligonucleotides and for assembling nucleic acid molecules using microfluidic devices.
    Type: Grant
    Filed: November 20, 2015
    Date of Patent: May 15, 2018
    Assignee: Gen9, Inc.
    Inventor: Larry Li-Yang Chu
  • Patent number: 9957472
    Abstract: A microfluidic platform for single-cell capture, stimulation, and imaging. It passively traps 4,000 single cells on a 4.5 mm2 footprint in 30 seconds, with a single-cell loading efficiency of 95%. The array format and optimized geometry allows for easy, robust and efficient single-cell loading, while maintaining captured cells in a low shear stress environment for long-term studies. Because cells are captured sequentially, the system is adequate for rare cell samples. Trapped cells can be exposed to various environmental conditions and chemical stimulus and their dynamic response can be monitored over time. The information gained from high-throughput, single-cell time lapsed imaging presents new opportunities in quantifying cellular responses, as averaged information by other measurement methods eliminates sub-population phenotypes.
    Type: Grant
    Filed: September 24, 2012
    Date of Patent: May 1, 2018
    Assignee: Georgia Tech Research Corporation
    Inventors: Kwanghun Chung, Catherine Rivet, Hang Lu, Melissa Kemp
  • Patent number: 9839893
    Abstract: The present invention provides microfabricated substrates and methods of conducting reactions within these substrates. The reactions occur in plugs transported in the flow of a carrier-fluid.
    Type: Grant
    Filed: August 26, 2015
    Date of Patent: December 12, 2017
    Assignee: The University of Chicago
    Inventors: Rustem F. Ismagilov, Joshua David Tice, Helen Song, Lewis Spencer Roach
  • Patent number: 9782736
    Abstract: A method and an apparatus for producing various types of microdroplets are provided. The apparatus has a cross intersection portion 7 at which a first continuous phase 2, a first dispersion phase 4, and a second dispersion phase 6 intersect with each other; a first liquid feed device 12 controlling the first dispersion phase 4; a second liquid feed device 13 controlling the second dispersion phase 6; and a control device 11 connected to the first liquid feed device 12 and the second liquid feed device 13, in which the first liquid feed device 12 and the second liquid feed device 13 are controlled by a signal from the control device 11 so that microdroplets 9 formed of the first dispersion phase 4 and microdroplets 10 formed of the second dispersion phase 6 are sequentially produced.
    Type: Grant
    Filed: April 25, 2014
    Date of Patent: October 10, 2017
    Assignee: JAPAN SCIENCE AND TECHNOLOGY AGENCY
    Inventors: Toru Torii, Toshiro Higuchi, Takashi Nishisako, Shingo Okushima
  • Patent number: 9757728
    Abstract: According to the invention, generally, a microfluidic aliquoting (MA) chip, adapted to fit in a Petri dish, has a center well (inlet) connected by a plurality of microchannels to a plurality of side wells (outlets). A relatively large (such as 120 ?L) cell suspension having several cells may be injected into the inlet of the MA chip, and single cells may be substantially simultaneously and uniformly distributed, via positive pressure-driving flow, to the several (such as 120) side wells having single cells in less than 1 minute. The MA Chip has a high efficiency in cell recovery. Due to rapid isolation and easy identification of single cells, high cell viability, high enrichment factor, and convenient transfer of submicroliter single-cell suspension, MA Chips are well compatible with CTC isolation from blood, single-cell cloning, PCR, and sequencing.
    Type: Grant
    Filed: January 26, 2016
    Date of Patent: September 12, 2017
    Inventors: Lidong Qin, Kai Zhang
  • Patent number: 9719971
    Abstract: The present disclosure relates to a microfluidic flame ionization detector for use in small scale separations, such as, for example, microfluidic gas chromatography and microfluidic carbon dioxide based fluid chromatography. In some arrangements, the microfluidic counter-current flame ionization detector employs a non-parallel arrangement for the introduction of combustion gases into the combustion chamber. In other arrangements, the detector housing is configured to incorporate at least one of the detector electrodes within the housing using electrically isolating fittings.
    Type: Grant
    Filed: September 11, 2014
    Date of Patent: August 1, 2017
    Assignee: Waters Technologies Corporation
    Inventors: Michael O. Fogwill, Joseph D. Michienzi, James P. Murphy, Geoff Gerhardt
  • Patent number: 9707554
    Abstract: The present invention relates to a disposable device (100) for amplifying at least one target nucleic acid present in a liquid and biological sample of interest, which consists of a solid body (2), at least one fluid channel (3) connecting an inlet (4), via which all or part of the sample of interest can be drawn up and/or discharged, and an outlet (5), which is itself connected to a means for the drawing up/discharging of the said sample of interest, the fluid channel (3) further comprising from the inlet (4) to the outlet (5): a first compartment (8) containing all or part of the thermostable constituents, a means (15) for mixing the constituents with the sample of interest, a second compartment (9) containing all or part of the non-thermostable constituents, and in addition, at least one zone intended for heating the said sample of interest (6) mixed with the said amplification constituents in order to allow the amplification of the target nucleic acid.
    Type: Grant
    Filed: September 17, 2010
    Date of Patent: July 18, 2017
    Assignee: bioMérieux, S.A.
    Inventors: Patrick Broyer, Laurent Drazek, Agnès Dupont Filliard, Michel Guy, Frédéric Pinston, Magaly Ponsard-Fillette, Thierry Kollaroczy
  • Patent number: 9637715
    Abstract: Microfluidic devices, systems, and methods providing for an invasion assay using microfluidic culture systems.
    Type: Grant
    Filed: April 1, 2012
    Date of Patent: May 2, 2017
    Assignee: EMD Millipore Corporation
    Inventors: Paul J. Hung, Philip J. Lee
  • Patent number: 9617520
    Abstract: Apparatuses, systems, and methods for generating concentration gradients of soluble molecules are disclosed herein. Devices and methods for generating in vitro blood vessels are also disclosed.
    Type: Grant
    Filed: September 16, 2015
    Date of Patent: April 11, 2017
    Assignee: SNU R&DB Foundation
    Inventors: Noo Li Jeon, Ju Hun Yeon, Qing Ping Hu, Sudong Kim, Hyun Jae Lee
  • Patent number: 9556470
    Abstract: The invention generally relates to methods for quantifying an amount of enzyme molecules. Systems and methods of the invention are provided for measuring an amount of target by forming a plurality of fluid partitions, a subset of which include the target, performing an enzyme-catalyzed reaction in the subset, and detecting the number of partitions in the subset. The amount of target can be determined based on the detected number.
    Type: Grant
    Filed: June 1, 2012
    Date of Patent: January 31, 2017
    Assignee: Raindance Technologies, Inc.
    Inventors: Darren Link, Michael L. Samuels
  • Patent number: 9530043
    Abstract: A cell analysis apparatus may include: an image processing unit imaging cultured cell colonies to convert the imaged cell colonies into digital signals; an outline extracting unit extracting outlines of the cell colonies from image data obtained from the image processing unit; a size calculating unit calculating sizes of the cell colonies from the extracted outlines; and a selecting unit comparing the sizes of the cell colonies with a preset value to select grown cell colonies.
    Type: Grant
    Filed: July 11, 2014
    Date of Patent: December 27, 2016
    Assignees: Samsung Electro-Mechanics Co., Ltd., Samsung Life Public Welfare Foundation
    Inventors: Dong Woo Lee, Do Hyun Nam, Bo Sung Ku, Yeon Sook Choi
  • Patent number: 9377440
    Abstract: An apparatus and method are disclosed for the precise selection and extraction of a selected analyte in a focused zone produced by isoelectric focusing performed in micro-channels. A cross-channel microfluidic device comprises a sample mixture introduction and separation channel and an extraction channel, which are in fluid communication with each other at a point of intersection. Means are provided for selectively moving the pattern of separated zones following cIEF to the intersection point, and means are provided for applying an extraction pressure to direct a single zone containing a selected analyte into and then out of the extraction channel for collection.
    Type: Grant
    Filed: October 3, 2012
    Date of Patent: June 28, 2016
    Assignee: ProteinSimple
    Inventors: Jiaqi Wu, Tiemin Huang, Arthur H. Watson
  • Patent number: 9315850
    Abstract: A method for transferring a filter membrane (130) bearing a retentate to a reagent pad (310), in which the membrane is mounted in a support frame (120) having a shoulder (121) and a skirt (122) projecting from the shoulder, the pad being mounted on a cassette (300) of complementary form to said skirt, the skirt being adapted to slide sealingly on the cassette until it comes into abutment formed by the shoulder, the method comprising the steps of sealingly engaging the skirt of the membrane support frame on the cassette, establishing a pressure difference between the cassette and the membrane support frame such that the membrane adopts the shape of a dome pointing towards the pad, while maintaining the pressure difference, applying a force on the frame so as to make the skirt slide on the cassette until contact is established between the end of the dome and the pad, maintaining a force on the frame so as to move it downward until it comes into abutment formed by the shoulder while maintaining the pressure diff
    Type: Grant
    Filed: January 12, 2011
    Date of Patent: April 19, 2016
    Assignee: EMD Millipore Corporation
    Inventors: Gael Waiche, Monika Kukuczka
  • Patent number: 9205432
    Abstract: Disclosed is an apparatus for self-extracting a cell using a magnetic field. The apparatus for self-extracting the cell using the magnetic field according to the present invention includes a flow path casing including an upper substrate and a lower substrate having a magnetic property combined with each other, and a fluid path formed to fluidize a cell solution therein; a separation portion disposed on the fluid path and provided with a separation channel selectively passing only an effective cell that is a separation target in the cell included in the cell solution therethrough; and a magnetic field control portion forming the magnetic field in the flow path portion to separate the cell blocking the separation channel from the separation channel.
    Type: Grant
    Filed: July 11, 2012
    Date of Patent: December 8, 2015
    Assignee: KOREA INSTITUTE OF MACHINERY & MATERIALS
    Inventors: Sung Hwan Chang, Yeong-Eun Yoo, Jung Yup Kim, Seung Min Hyun, Kyung-Hyun Whang
  • Patent number: 9132394
    Abstract: System, including methods and apparatus, for detection of spaced droplets.
    Type: Grant
    Filed: March 25, 2011
    Date of Patent: September 15, 2015
    Assignee: Bio-Rad Laboratories, Inc.
    Inventors: Anthony J. Makarewicz, Jr., Amy L. Hiddessen
  • Patent number: 9086371
    Abstract: The invention relates to fluidics as used in medical and diagnostic equipment and relates further to means for purifying, abstracting, filtering, detecting and/or measuring analytes in liquid samples.
    Type: Grant
    Filed: July 17, 2012
    Date of Patent: July 21, 2015
    Assignee: OSMETECH TECHNOLOGY, INC.
    Inventors: Charles E Clemens, Robert Mucic, Rudolph A. Montalvo, Clark Foster, Gary R. Gust, Thomas P. Robinson, Gary T. Olsen
  • Patent number: 9067189
    Abstract: A microfluidic device includes a plurality of reagent sources for a feeding a plurality of reagents, each reagent source feeding a corresponding reagent among the plurality of reagents. A macro-chamber receives one or more reagents among the plurality of reagents from the plurality of reagent sources. A microfluidic reactor is coupled to the macro-chamber and the plurality of reagent sources and configured to receive one or more reagents among the plurality of reagents from at least one of the macro-chamber, the plurality of reagent sources, and react the one or more reagents to generate reaction content. The macro-chamber is further configured to receive the reaction content from the microfluidic reactor. A system configured to control at least one of flow of a dry gas in and out of the macro-chamber, surface area of at least one of the one or more reagents, and the reaction content in the macro-chamber.
    Type: Grant
    Filed: March 30, 2012
    Date of Patent: June 30, 2015
    Assignee: General Electric Company
    Inventors: Victor Donald Samper, Ruben Julian Horvath-Klein, Marko Klaus Baller, Christian Friedrich Peter Rensch, Christoph Boeld
  • Patent number: 9040288
    Abstract: This patent application describes an integrated apparatus for processing polynucleotide-containing samples, and for providing a diagnostic result thereon. The apparatus is configured to receive a microfluidic cartridge that contains reagents and a network for processing a sample. Also described are methods of using the apparatus.
    Type: Grant
    Filed: March 26, 2007
    Date of Patent: May 26, 2015
    Assignee: HandyLab, Inc.
    Inventors: Kalyan Handique, Sundaresh N. Brahmasandra, Karthik Ganesan, Betty Wu, Nikhil Phadke, Gene Parunak, Jeff Williams
  • Patent number: 9040285
    Abstract: A sensing device including an inlet port for receiving a fluid, a measurement chamber for sensing the fluid, a fluid channel coupling the inlet port and the measurement chamber for transporting the fluid from the inlet port to the measurement chamber, and a fluid stop unit for stopping and controllably releasing the flow of fluid between the inlet port and the measurement chamber.
    Type: Grant
    Filed: December 7, 2009
    Date of Patent: May 26, 2015
    Assignee: Koninklijkle Philips N.V.
    Inventors: Albert Hendrik Jan Immink, Albert F. Gootzen, Dirk Jan Nelly Vananderoye, Petrus Johannes Wilhelmus Van Lankvelt
  • Patent number: 9023641
    Abstract: The present invention relates to an leucocyte antigen mediated microfluidic assay and a microfluidic device for analyzing a subjects' body fluids containing leucocytes to determine if the subject has been previously exposed to a predetermined antigen.
    Type: Grant
    Filed: May 13, 2005
    Date of Patent: May 5, 2015
    Assignee: Advanced Animal Diagnostics
    Inventors: Rodolfo R. Rodriguez, Douglas J. Darr
  • Patent number: 9023640
    Abstract: A portable system for real time detection of the presence of an infectious agent in a biological sample employs a reagent which detects the presence of a specific infectious agent in the sample, and emits a detectable signal when the reagent reacts with the sample and detects the presence of the infectious agent. A test cartridge has a reaction chamber for receiving the sample and the reagent. The reaction chamber has a predetermined internal geometry and at least one inner surface. Introducing the sample and the reagent into the test cartridge mixes the sample and the reagent. A testing unit receives the test cartridge, and includes a sensor for detecting an emitted detectable signal. The detection of the emitted detectable signal is indicative of the presence of the infectious agent in the sample.
    Type: Grant
    Filed: December 11, 2012
    Date of Patent: May 5, 2015
    Assignee: Fundamental Solutions Corporation
    Inventors: Marvin R. Williams, Charles McBrairty, Daniel W. Pfautz, Thomas J. Zupancic, Lingchun Zeng, Andrew Weiman, Richard S. Brody, Joseph Kittle, Anthony Truscott, Robert Baranowski
  • Patent number: 9017995
    Abstract: A liquid-transport device has a liquid-tight support (12) on which there is applied a start zone (24; 24?) for applying transport liquid to be transported and a target zone (26, 28; 26a-e) into which the transport liquid is to be transported and also a conduction zone that extends between the start zone (24, 24?) and the target zone (26, 28; 26a-e) and that has a microporous transport layer (14) in which the transport liquid flows by capillary force from the start zone (24; 24?) to the target zone (26, 28; 26a-e). The conduction zone has a multiplicity of open flow channels separated from one another by microporous bridges having open-pored side walls.
    Type: Grant
    Filed: May 19, 2011
    Date of Patent: April 28, 2015
    Assignee: Sartorius Stedim Biotech GmbH
    Inventors: Karl Pflanz, Eric Jallerat, Darius Haeusler, Markus Hollas, Uwe Andag
  • Patent number: 9017941
    Abstract: A method for nucleic acid analysis including injecting a slight amount of nucleic acid sample for analysis, characterized in that most of a nucleic acid sample which is not used, excluding the slight amount of the nucleic acid sample which is used for analysis, can be obtained as a pure product which is not contaminated with a fluorescent material, and a microchip for analyzing nucleic acid which enables such method for nucleic acid analysis, are provided. The method for nucleic acid analysis may analyze at least two different nucleic acid samples in a continuous manner by sequentially injecting the at least two different nucleic acid samples.
    Type: Grant
    Filed: March 7, 2013
    Date of Patent: April 28, 2015
    Assignee: Postech Academy-Industry Foundation
    Inventors: Jong Hoon Hahn, Byoung Joo Kwak, Han-Ok Kim
  • Patent number: 9012208
    Abstract: A self-contained apparatus for isolating nucleic acid, cell lysates and cell suspensions from unprocessed samples apparatus, to be used with an instrument, includes at least one input, and: (i) a macrofluidic component, including a chamber for receiving an unprocessed sample from a collection device and at least one filled liquid purification reagent storage reservoir; and (ii) a microfluidic component in communication with the macrofluidic component through at least one microfluidic element, the microfluidic component further comprising at least one nucleic acid purification matrix; and (iii) at least one interface port to a drive mechanism on the instrument for driving said liquid purification reagent, through the microfluidic element and the nucleic acid purification matrix, wherein the only inputs to the apparatus are through the chamber and the interface port to the drive mechanism.
    Type: Grant
    Filed: February 3, 2010
    Date of Patent: April 21, 2015
    Assignee: NetBio, Inc.
    Inventors: Richard F. Selden, Eugene Tan
  • Patent number: 9005991
    Abstract: Devices and methods for testing a biological sample for an analyte of interest are provided that make use of a reaction chamber for receiving a reaction mixture, a housing positioned below the reaction chamber for receiving a test cassette, and a door that is positioned in the top portion of the housing. The door includes a ramp that extends downwardly into the housing such that biasing the ramp upwards opens the door into the cavity of the reaction chamber and places the reaction chamber in fluid communication with the insertion area to thereby allow the reaction mixture to flow onto a test cassette and allow for the determination of an amount of an analyte of interest.
    Type: Grant
    Filed: January 5, 2012
    Date of Patent: April 14, 2015
    Assignee: American Bio Medica Corporation
    Inventors: Douglas Casterlin, Larry Ferringo
  • Patent number: 8999636
    Abstract: A chamber in which an agent, like genomic DNA, may be harvested and optionally manipulated rapidly (e.g., on the order of a few hours), without shearing or fragmentation.
    Type: Grant
    Filed: January 8, 2008
    Date of Patent: April 7, 2015
    Assignee: Toxic Report LLC
    Inventors: Vishal Patil, Emilia Mollova, Rudolf Gilmanshin
  • Patent number: 8993311
    Abstract: A disposable cartridge is described which is equipped with a plurality of microfabricated particle sorting structures. The disposable cartridge may include passageways which connect fluid reservoirs in the cartridge with corresponding microfluidic passageways on the particle sorting structure. A flexible gasket may prevent leakages and allow the fluid to cross the gasket barrier through a plurality of holes in the gasket, allowing fluid to be transferred from the reservoirs to the microfabricated particle sorting structures. The plurality of particle sorting structures may be arranged in the disposable cartridge in order to perform multiple separation operations, such as a sequential or parallel sorting operation.
    Type: Grant
    Filed: May 23, 2012
    Date of Patent: March 31, 2015
    Assignee: Innovative Micro Technology
    Inventors: John S. Foster, Daryl W. Grummitt, John C. Harley, James P. Linton, Jaquelin K. Spong, Douglas L. Thompson
  • Patent number: 8993310
    Abstract: A device for diagnosis of physiologic status and/or selection of best spermatozoa of a semen sample based on chemotaxis, and the procedure of thereof, enabling by a simple and inexpensive device the diagnosis and selection of the best spermatozoa in only one step. Only needed are: the present device, a regular light microscope, and personnel with elementary knowledge of laboratory management. The device is of the type having two communicated compartments (1a, 1b), and where said compartments (1a, 1b) communication occurs through a duct or bridge (2) located in the lower part, and above the lower level of mentioned compartments (1a, 1b); in the entrances of said compartments (1a, 1b) appropriate closing means (3, 4) and appropriate air output ducts (5) are placed communicating the top end of compartments (1a, 1b) with the exterior.
    Type: Grant
    Filed: March 3, 2010
    Date of Patent: March 31, 2015
    Assignees: Consejo Nacional de Investigaciones Cientificas y Technicas (CONICET), Inis Biotech LLC
    Inventors: Laura Cecilia Giojalas, Héctor Alejandro Guidobaldi, Laura Virginia Gatica, Maria Eugenia Teves, Maria del Mar Montesinos, Diego Rafael Uñates