Including A Dish, Plate, Slide, Or Tray Patents (Class 435/288.3)
  • Patent number: 10870887
    Abstract: Described are methods and kits for identifying a subject at risk of, or having, a sensory neuropathy related disease, such as sensory neuropathies. In particular, the disclosure is based on the determination of mutations in the SPTLC2 gene causing sensory neuropathies.
    Type: Grant
    Filed: November 16, 2017
    Date of Patent: December 22, 2020
    Assignees: VIB VZW, UNIVERSITEIT ANTWERPEN, UNIVERSITY OF ZURICH, MEDICAL UNIVERSITY OF GRAZ
    Inventors: Annelies Rotthier, Vincent Timmerman, Michaela Auer-Grumbach, Thorsten Hornemann
  • Patent number: 10872414
    Abstract: An information processing apparatus according to an embodiment of the present technology includes a control unit. The control unit detects, on a basis of an optical image of a cell in culture captured at a first imaging interval, whether there is a change in a state of the cell, and switches, when detecting the change in the state, an imaging mode from the first imaging interval to a second imaging interval shorter than the first imaging interval.
    Type: Grant
    Filed: May 18, 2017
    Date of Patent: December 22, 2020
    Assignee: Sony Corporation
    Inventors: Suguru Aoki, Takeshi Ohashi, Naofumi Matsui, Tomoya Onuma
  • Patent number: 10815522
    Abstract: Pancreatic adenocarcinoma has the worst overall mortality of any solid tumor, with only 7% of patients surviving after 5 years. To evaluate the clinical implications of genomic alterations in this low cellularity tumor type, we deeply sequenced the genomes of 101 enriched pancreatic adenocarcinomas from patients who underwent potentially curative resections and used non-invasive approaches to examine tumor specific mutations in the circulation of these patients. These analyses revealed somatic mutations in chromatin regulating genes including MLL and ARID1A in 20% of patients that were associated with improved survival. Liquid biopsy analyses of cell free plasma DNA revealed that 43% of patients with localized disease had detectable circulating tumor DNA (ctDNA) in their blood at the time of diagnosis. Detection of ctDNA after resection predicted clinical relapse and poor outcome, and disease recurrence by ctDNA was detected 6.5 months earlier than with standard CT imaging.
    Type: Grant
    Filed: February 18, 2016
    Date of Patent: October 27, 2020
    Assignee: The Johns Hopkins University
    Inventors: Victor Velculescu, Mark Sausen, Vilmos Adleff, Jillian Phallen
  • Patent number: 10799523
    Abstract: The present invention relates to oligomer compounds (oligomers), which target Tau mRNA in a cell, leading to reduced expression of Tau protein. Reduction of Tau protein expression is beneficial for the treatment of certain medical disorders, e.g., a neurological disorder.
    Type: Grant
    Filed: February 4, 2016
    Date of Patent: October 13, 2020
    Assignee: F. HOFFMANN-LA ROCHE AG
    Inventors: Richard E. Olson, Angela M. Cacace, Peter Hagedorn, Anja Mølhart Høg, Dong Li, Jeffrey M. Brown, Marianne Lerbech Jensen, Niels Fisker Nielsen, Stephen E. Mercer
  • Patent number: 10787664
    Abstract: The present disclosure relates to isolated compounds including a nucleic acid sequence capable of hybridizing to an RNA sequence 10 to 270 nucleobases downstream of the transcription start site of a mammalian microRNA-379 transcript; method of treating diabetic nephropathy in a subject with the compounds; and method of inhibiting expression of a mammalian microRNA-379 megacluster with the compounds.
    Type: Grant
    Filed: May 25, 2016
    Date of Patent: September 29, 2020
    Assignee: City of Hope
    Inventors: Rama Natarajan, Mitsuo Kato
  • Patent number: 10760131
    Abstract: Disclosed are methods of, and assay and kits for, detecting and diagnosing arterial plaque rupture using the expression of miR-222, mi-221 and circ284. Also disclosed are methods of treating arterial plaque rupture and methods of identifying agents for use thin the treatment of arterial plaque rupture.
    Type: Grant
    Filed: February 23, 2016
    Date of Patent: September 1, 2020
    Inventors: Thomas Cooper Woods, Hernan Antonio Bazan
  • Patent number: 10739345
    Abstract: A splice variant of bcr-abl mRNA that produces BCR-ABL protein with a truncated C-terminus and its role in resistance to treatment with kinase inhibitors is disclosed. Vectors for expressing the truncated gene product are provided as well as recombinant cells that express the truncated gene product from a cDNA construct. Also provided are methods compositions and kits for detecting the BCR-ABL splice variant. Additionally, methods for screening BCR-ABL kinase domain inhibitors which rely on the recombinant cells and methods of predicting likelihood for resistance of a CML patient with a BCR/ABL translocation respond to treatment with one or more BCR-ABL kinase inhibitors are also disclosed.
    Type: Grant
    Filed: July 7, 2017
    Date of Patent: August 11, 2020
    Assignee: Quest Diagnostics Investments Incorporated
    Inventor: Maher Albitar
  • Patent number: 10712276
    Abstract: A imaging device 1-D nanomaterials is provided. The device includes: a first light source, a second light source, a microscope with a liquid immersion object, and a carrier. The first light source is configured to provide a first incident light and the second light source is configured to provide a second incident light, the first incident light and the incident light are not parallel to each other. The carrier is configured to contain a 1-D nanomaterials sample and a liquid, both the 1-D nanomaterials sample and the liquid immersion object are immersed in the liquid.
    Type: Grant
    Filed: August 29, 2018
    Date of Patent: July 14, 2020
    Assignees: Tsinghua University, HON HAI PRECISION INDUSTRY CO., LTD.
    Inventors: Wen-Yun Wu, Dong-Qi Li, Jin Zhang, Kai-Li Jiang, Shou-Shan Fan
  • Patent number: 10697018
    Abstract: A method for assessing the predisposition of a subject to weight loss attainable by applying one or more dietary interventions to a subject and/or the predisposition of a subject to maintenance of weight loss following one or more dietary interventions which method comprises determining the nucleotide of the subject at one or more polymorphic positions selected from: (i) position 101 of SEQ ID NO:1 (rs953211) (ii) position 101 of SEQ ID NO:2 (rs1509290) (iii) position 101 of SEQ ID NO:3 (rs1509289) and/or detecting one or more biomarkers genetically linked to said polymorphic positions.
    Type: Grant
    Filed: November 9, 2015
    Date of Patent: June 30, 2020
    Assignee: Societe des Produits Nestle S.A.
    Inventors: Armand Valsesia, Jorg Hager
  • Patent number: 10640742
    Abstract: A hydraulic cell stretching device comprising a source of variable pressured hydraulic fluid hydraulically coupled to a flexing chamber. The flexing chamber has at least one cell well. The cell well has a membrane subjected to the variable pressured hydraulic fluid.
    Type: Grant
    Filed: September 25, 2015
    Date of Patent: May 5, 2020
    Assignee: Board of Supervisors of Louisiana State University and Agricultural and Mechanical College
    Inventors: Kevin John McCarthy, Deborah Jane Wassenhove-McCarthy
  • Patent number: 10533998
    Abstract: The invention generally relates to methods for quantifying an amount of enzyme molecules. Systems and methods of the invention are provided for measuring an amount of target by forming a plurality of fluid partitions, a subset of which include the target, performing an enzyme-catalyzed reaction in the subset, and detecting the number of partitions in the subset. The amount of target can be determined based on the detected number.
    Type: Grant
    Filed: June 1, 2012
    Date of Patent: January 14, 2020
    Assignee: Bio-Rad Laboratories, Inc.
    Inventors: Darren R. Link, Michael L. Samuels
  • Patent number: 10493455
    Abstract: An apparatus for isolating corneal endothelial cells (CECs) includes a housing with an interior wall that defines a hollow interior of the housing. At least four fluid channels are formed in the housing with two of the channels terminating in respective openings in an upper portion of the interior wall and two of the channels terminating in respective jets in the upper portion of the interior wall. A convex projection is centrally located on the circular base of the hollow interior and is configured to receive an inverted cornea. A circular insert for the housing has an opening therein in which the convex projection projects when the insert is mated with the lower portion of the interior wall. CECs are harvested from the inverted cornea.
    Type: Grant
    Filed: October 26, 2018
    Date of Patent: December 3, 2019
    Assignee: The Government of the United States, as represented by the Secretary of the Army
    Inventors: Robert Haggins, Jr., Erik Eaton, Jr., Zachary Murray, Timothy Varney
  • Patent number: 10407740
    Abstract: Oligomers useful for determining the presence of Trichomonas vaginalis in a test sample.
    Type: Grant
    Filed: May 22, 2017
    Date of Patent: September 10, 2019
    Assignee: GEN-PROBE INCORPORATED
    Inventors: Barbara Susan Weinbaum, Janel M. Dockter, Astrid R. W. Schroder
  • Patent number: 10351911
    Abstract: Provided are methods and kits for determining predisposition of a subject to develop a kidney disease, by identifying in a sample of the subject at least one APOL1 polypeptide variant which is characterized by a higher trypanolytic activity on Trypanosoma brucei rhodesiense as compared to the trypanolytic activity of wild type APOL1 polypeptide as set forth in SEQ ID NO:1 on the Trypanosoma brucei rhodesiense under identical assay conditions; or at least one APOL1 nucleotide mutation in the APLO1 genomic sequence set forth in SEQ ID NO:3, wherein the at least one nucleotide mutation or polypeptide variant being in linkage disequlibrium (LD) with the S342G mutation in the APOL1 polypeptide set forth in SEQ ID NO:1, wherein presence of the APOL1 polypeptide variant indicates increased predisposition of the subject to develop the kidney disease.
    Type: Grant
    Filed: April 24, 2016
    Date of Patent: July 16, 2019
    Assignees: Technion Research & Development Foundation Limited, Ramot at Tel-Aviv University Ltd., Walter Gabriel WASSER, Rambam Med-Tech Ltd.
    Inventors: Karl Skorecki, Shay Tzur, Saharon Rosset, Walter Gabriel Wasser, Doron M. Behar, Revital Shemer
  • Patent number: 10240118
    Abstract: A system for monitoring growth media within a controlled environment is provided and includes a well plate having a plate top and a plate side and defines at least one well cavity. The plate top includes a top opening and the plate side includes a side opening and the side opening is communicated with one of the at least one well cavity. The system includes a sensor assembly unit that includes a unit structure defining a reference material chamber containing a reference material, a sensor chamber having a chamber opening, and a base chamber. Additionally, the system includes a reference electrode communicated with the reference material and the base chamber. A media sensor is provided and is communicated with the chamber opening and a media sensor electrode communicated with the media sensor. The reference electrode and media sensor electrode are communicated with a processing device.
    Type: Grant
    Filed: April 28, 2016
    Date of Patent: March 26, 2019
    Inventor: Mark Langley
  • Patent number: 10233506
    Abstract: Compositions and methods for detecting Trichomonas vaginalis are provided.
    Type: Grant
    Filed: July 15, 2014
    Date of Patent: March 19, 2019
    Assignee: Cepheid
    Inventors: James Wang, Sudhir Alugupally, Rosa Yu, Sally Yousif
  • Patent number: 10138454
    Abstract: A cell-culture container includes a container main body and a presser member. The container main body has a recessed part for containing a cell-culture solution. The presser member is a member that is removably fitted onto an upper side of the recessed part. The presser member has a lower surface, which makes contact with an upper surface of the cell-culture solution in a fitted state, and a bubble-discharge part, which enables the discharge of bubbles in the cell-culture solution to the exterior. Such an arrangement enables accurate observation by the elimination of a meniscus in a culture container.
    Type: Grant
    Filed: June 10, 2015
    Date of Patent: November 27, 2018
    Assignee: NISSHA PRINTING CO., LTD.
    Inventors: Naoko Obi, Atsushi Onishi, Toshihiro Iga
  • Patent number: 10131895
    Abstract: The present disclosure provides for a polynucleotide sequences encoding a xylanase. More specifically, the present disclosure provides for polynucleotide sequences with codon mutations encoding a xylanase.
    Type: Grant
    Filed: March 10, 2014
    Date of Patent: November 20, 2018
    Assignee: BASF Enzymes LLC
    Inventor: Xuqiu Tan
  • Patent number: 10036074
    Abstract: This invention is related to the area of characterization of inflammation in relation with the gut microbiota, in metabolic and autoimmune disorders. In particular, it relates to the identification of gene signatures which can be used as a marker predictive of inflammation associated diseases, such as liver-related metabolic disorders, in particular to the evolution of benign steatosis towards its most severe forms (steatohepatitis and cirrhosis) or autoimmune disorders, in particular inflammatory bowel diseases (Crohn's and Ulcerative Colitis). These gene signatures can therefore be used as a means of diagnosis, prognosis, stratification for drug studies, for monitoring patient and for assigning an appropriate treatment.
    Type: Grant
    Filed: October 17, 2013
    Date of Patent: July 31, 2018
    Assignees: INSTITUT PASTEUR, INSTITUT NATIONAL DE LA SANTE ET DE LA RECHERCHE MEDICALE (INSERM)
    Inventors: Matthieu Pichaud, Pierre Rimbaud, Stanislav Ehrlich
  • Patent number: 10036071
    Abstract: Methods for detecting the amplifications of sequences in the BRCA1 locus, which sequences have ends consisting of or are framed with sequence stretches present at least twice in the BRCA1 locus, and which amplification results in at least two or at least three, especially three, tandem copies of the amplified sequence; methods for determining a predisposition to diseases or disorders associated with these amplifications, including predisposition to ovarian cancer or breast cancer and methods for detecting amplifications with similar features in other loci.
    Type: Grant
    Filed: March 14, 2014
    Date of Patent: July 31, 2018
    Assignee: GENOMIC VISION
    Inventors: Maurizio Ceppi, Jennifer Abscheidt, Emmanuel Conseiller, Etienne Rouleau
  • Patent number: 10030271
    Abstract: Using an RT-PCR platform, detection of gene transcripts highly expressed in prostate tissue and expressed in peripheral blood mononuclear cells (PBMC) from patients with mCRPC can provide a more reliable and robust prediction of poor overall survival than that of CTC enumeration in mCRPC. Disclosed is the identification of five genes, KLK3, KLK2, HOXB13, GHRL2 and FOXA1, the detection of two (2) or more transcripts of which predicts overall poor survival. The test is performed on blood samples that have been collected in collection tubes that stabilize intracellular RNA, require minimal on-site processing and can be easily stored and shipped for subsequent extraction of total RNA from whole blood for RT-PCR.
    Type: Grant
    Filed: August 19, 2013
    Date of Patent: July 24, 2018
    Assignee: MEMORIAL SLOAN-KETTERING CANCER CENTER
    Inventors: Howard Scher, Martin Fleisher, Daniel Danila
  • Patent number: 10029253
    Abstract: Micro droplets are accurately placed in a reaction well. The reaction well (5) is formed in one surface of a well base (3). A channel base (11) is placed on the well base (3). The channel base (11) has, in its surface joined to the well base (3), a liquid introduction channel (12a) and a reaction well air vent channel (18a). The channel base (11) also has a recess (27) formed opposite to the reaction well (5) and recessed upward from the upper surface of the liquid introduction channel (12a). When viewed from above, a shoulder part (26) of the recess (27) is placed near the connection part between the reaction well (5) and the liquid introduction channel (12a) and is closer to the center of the reaction well (5) than a shoulder part (16) of the reaction well (5).
    Type: Grant
    Filed: October 11, 2013
    Date of Patent: July 24, 2018
    Assignee: SHIMADZU CORPORATION
    Inventors: Masakazu Akechi, Masaki Kanai, Takahiro Nishimoto, Nobuhiro Hanafusa
  • Patent number: 10017830
    Abstract: The present invention provides a group of specific probes directed to cleavage site of hemagglutinin precursor protein of avian influenza virus subtypes H5, and provides a method for rapid pathotyping of H5 avian influenza virus. The present invention further provides a kit containing the probes and the kit is easy-to-use, low-cost, high sensitivity, enabled the molecular pathotyping of H5 viruses by a simpler and faster means that conventional methods.
    Type: Grant
    Filed: May 23, 2014
    Date of Patent: July 10, 2018
    Assignee: National Taiwan University
    Inventors: Lih-Chiann Wang, Chien-Hao Huang, Ching-Ho Wang
  • Patent number: 9994916
    Abstract: Methods and compositions for detection of Neisseria gonorrhoeae are disclosed herein. In some embodiments, the presence or absence of N. gonorrhoeae in a sample is determined using nucleic acid-based testing methods using primers and/or probes that bind to opcA gene region of N. gonorrhoeae.
    Type: Grant
    Filed: March 7, 2014
    Date of Patent: June 12, 2018
    Assignee: Becton, Dickinson and Company
    Inventors: Keith Edward Thornton, Paul Madepogu, Danielle Koffenberger
  • Patent number: 9982305
    Abstract: This invention relates to the use of human neutrophil marker interleukin 8 receptor B (IL8Rb) for detection of bladder cancer, and more particularly to the use of EL8Rb for discriminating between transitional cell carcinoma of the bladder (TCC) and inflammatory conditions of the bladder.
    Type: Grant
    Filed: November 11, 2011
    Date of Patent: May 29, 2018
    Assignee: PACIFIC EDGE LIMITED
    Inventors: Parry John Guilford, Mark Dalphin, Laimonis Kavalieris, Paul O'Sullivan
  • Patent number: 9976191
    Abstract: The present invention provides a rice whole genome breeding chip and the application thereof. The rice whole genome breeding chip of the present invention is Rice60K, an SNP chip manufactured based on Infinium technique. Each chip can detect 24 samples simultaneously and contains 58,290 SNP sites. The marker sites have DNA sequences represented by SEQ ID NO.1-58290. The chip can be used in molecular marker fingerprint analysis of the rice variety resources, in genotype identification of the hybrid progeny population, in identification of the variety authenticity, in analysis and screening of the genetic background of the breeding materials, and in association analysis of the agronomic traits, having wide application prospects.
    Type: Grant
    Filed: February 7, 2013
    Date of Patent: May 22, 2018
    Assignees: China National Seed Group Co., Ltd., Frontier Laboratories of Systems Crop Design Co., Ltd., Huazhong Agricultural University, Shenzhen Institute of Molecular Crop Design
    Inventors: Fasong Zhou, Haodong Chen, Weibo Xie, Hang He, Huihui Yu, Xiaoyan Tang, Jing Li, Junli Zhou, Yuqing He, Wei Chen, Qifa Zhang, Xingwang Deng
  • Patent number: 9783807
    Abstract: A nucleic acid molecule utilizable for Salmonella detection is provided. The nucleic acid molecule which binds to Salmonella includes any of the following polynucleotides (a) to (d): (a) a polynucleotide composed of any of base sequences of SEQ ID NOs: 1 to 17; (b) a polynucleotide composed of a base sequence obtained by deletion, substitution, insertion, and/or addition of one or more bases in any of the base sequences in the polynucleotide (a) and is bound to Salmonella; (c) a polynucleotide composed of a base sequence having an identity of 80% or more to any of the base sequences in the polynucleotide (a) and is bound to Salmonella; and (d) a polynucleotide composed of a base sequence complementary to a polynucleotide which hybridizes to the polynucleotide (a) composed of any of the base sequences under stringent conditions and is bound to Salmonella.
    Type: Grant
    Filed: November 6, 2013
    Date of Patent: October 10, 2017
    Assignee: NEC SOLUTION INNOVATORS, LTD.
    Inventors: Hirotaka Minagawa, Jou Akitomi, Naoto Kaneko, Makio Furuichi, Katsunori Horii, Iwao Waga
  • Patent number: 9765291
    Abstract: An apparatus for collecting or culturing cells or cell colonies includes: a common substrate formed from a flexible resilient polymeric material and having a plurality of wells formed therein; and a plurality of rigid cell carriers releasably connected to said common substrate, with said carriers arranged in the form of an array, and with each of the carriers resiliently received in one of the wells. A method of collecting or culturing cells or cell colonies with such an apparatus is carried out by depositing a liquid media carrying cells on the apparatus so that said cells settle on or adhere to said the carriers; and then (c) releasing at least one selected carrier having said cells thereon by gradual application of release energy to each carrier from the cavity in which it is received (e.g., by pushing with a probe).
    Type: Grant
    Filed: May 18, 2015
    Date of Patent: September 19, 2017
    Assignee: The University of North Carolina at Chapel Hill
    Inventors: Nancy Allbritton, Christopher Sims, Yuli Wang, Pavak Kirit Shah
  • Patent number: 9709548
    Abstract: Systems and methods for improved monitoring of excitation-contraction coupling and excitable cells are provided, which provide millisecond time resolution. The system is capable of continuously monitoring excitation-contraction coupling in a relatively high-throughput manner. The system includes a device for monitoring cell-substrate impedance, an impedance analyzer capable of impedance measurements at millisecond time resolution, electronic circuitry that can engage the device and selectively connect two or more electrode arrays of the device to the impedance analyzer and a software program that controls the electronic circuitry and records and analyzes data obtained from the impedance analyzer.
    Type: Grant
    Filed: May 5, 2009
    Date of Patent: July 18, 2017
    Assignee: ACEA Biosciences, Inc.
    Inventors: Xiaobo Wang, Yama A. Abassi, Biao Xi, Wen Fu Zhang, Xiao Xu
  • Patent number: 9670554
    Abstract: Oligomers useful for determining the presence of Trichomonas vaginalis in a test sample.
    Type: Grant
    Filed: July 21, 2014
    Date of Patent: June 6, 2017
    Assignee: GEN-PROBE INCORPORATED
    Inventors: Barbara Susan Weinbaum, Janel M. Dockter, Astrid R. W. Schroder
  • Patent number: 9624556
    Abstract: Described herein are novel polynucleotides associated with viral infections. The polynucleotides are miRNAs and miRNA precursors. Related methods and compositions that can be used for diagnosis, prognosis, and treatment of those medical conditions are disclosed. Also described herein are methods that can be used to identify modulators of viral infections.
    Type: Grant
    Filed: July 13, 2015
    Date of Patent: April 18, 2017
    Assignee: Rosetta Genomics Ltd.
    Inventors: Itzhak Bentwich, Amir Avniel, Yael Karov, Ranit Aharonov
  • Patent number: 9612234
    Abstract: A method of determining one or more beating parameters for use in cardiomyocyte beating analysis including: providing a cell analysis device including wells, each well including a sensor capable of monitoring beating of cardiomyoctes in millisecond time resolution; adding cardiomyocytes to the wells; monitoring the beating of the cardiomyocytes in millisecond time resolution to obtain a plurality of beating measurements; and calculating one or more beating parameters from the plurality of beating measurements.
    Type: Grant
    Filed: May 17, 2011
    Date of Patent: April 4, 2017
    Assignee: ACEA Biosciences, Inc.
    Inventors: Nan Li, Xiaobo Wang, Yama A. Abassi, Biao Xi, Wen Fu Zhang, Xiao Xu
  • Patent number: 9506105
    Abstract: A method for amplifying a target nucleic acid present in a sample includes introducing, via an entrance opening of a planar fluidic assembly, a sample containing one or more target nucleic acids into a flow channel of the planar fluidic assembly, the flow channel extending from the entrance opening to an exit vent and having a substantially uniform cross-section. A plurality of nucleic acid primers that are complementary to a portion of the one or more target nucleic acids are disposed at locations within and along the flow channel. The method further includes subjecting the sample introduced into the flow channel to a primer-based amplification reaction using the nucleic acid primers, wherein the primer-based amplification reaction produces amplified product of the one or more target nucleic acids, and retaining the amplified product at one or more of the locations within the flow channel during the primer-based amplification reaction.
    Type: Grant
    Filed: August 11, 2014
    Date of Patent: November 29, 2016
    Assignees: APPLIED BIOSYSTEMS, LLC, UNITED STATES OF AMERICA, AS REPRESENTED BY THE SECRETARY, DEPARTMENT OF HEALTH AND HUMAN SERVICES
    Inventors: James F. Brown, Jonathan E. Silver
  • Patent number: 9447474
    Abstract: Provided is a method for determining the presence or absence of an individual's DNA in a sample containing DNA from two or more contributors. A panel of a plurality of single nucleotide polymorphisms (SNPs) is used. For each SNP in the panel, it is determined whether the minor allele of the SNP is present in the sample, and whether the minor allele is present in the individual's DNA. If the number of minor alleles that are present in the individual's DNA that are also present in the DNA sample is above a predetermined threshold, the individual's DNA is concluded to be present in the sample. Also provided is an array of DNA molecules for use in the method, as well as a method for estimating the number of individuals contributing to a DNA containing sample.
    Type: Grant
    Filed: December 2, 2010
    Date of Patent: September 20, 2016
    Assignee: YISSUM RESEARCH DEVELOPMENT COMPANY OF THE HEBREW UNIVERSITY OF JERUSALEM, LTD.
    Inventors: Ariel Darvasi, Lev Voskoboinik
  • Patent number: 9347097
    Abstract: An arrangement for determining the concentration of nucleic acids in a sample in a microfluidic device. In at least one embodiment, the arrangement having a) a first chamber into which a the sample is introduced, b) an element in the first chamber for carrying out a number of cycles of an amplification reaction to be carried out in cycles for amplifying nucleic acids, c) a second chamber, connectable to the first chamber by way of fluidic communication, equipped with an element to determine concentrations of amplified nucleic acids, d) a control unit to introduce a defined volume of a solution containing reagents for the amplification reaction into the first chamber and to transfer a defined volume from said first chamber to the second chamber.
    Type: Grant
    Filed: March 20, 2014
    Date of Patent: May 24, 2016
    Assignee: Boehringer Ingelheim Vetmedica GmbH
    Inventors: Walter Gumbrecht, Jörn Mosner, Sebastian Schmidt
  • Patent number: 9342734
    Abstract: The present invention generally relates to systems and methods for counting biomolecules or cells. In certain embodiments, the invention provides a cell counting or biomolecule counting system including: a covered chamber having a known height and configured to hold a suspension of biomolecules or cells in a sample; at least one fluorescent light source connected to at least one fluorescent light beam narrowing device; a bright-field light source connected to a bright-field light beam narrowing device; a microscope objective; a detection device; a fluorescent filter assembly to allow only excitation light to illuminate the sample and allow only emission light from the sample to be imaged by the detection device; and a movable light shutter to block bright-field light during fluorescent detection.
    Type: Grant
    Filed: August 6, 2014
    Date of Patent: May 17, 2016
    Assignee: Nexcelom bioscience LLC
    Inventors: Bo Lin, Peter Y. Li, Jean Qiu, Timothy Smith, Todd Sobolewski, Alnoor Pirani
  • Patent number: 9103785
    Abstract: A method and apparatus for performing melting curve analyses of nucleic acids on a microarray is described. The present method includes varying the temperature of a fluid on a microarray to dissociate and remove target DNA, scanning the mircoarray for fluorescence, collecting the target DNA removed from the microarray, and reusing the collected target DNA and the microarray. The apparatus of the present disclosure includes a microarray stage, a light source and detector, and a temperature controller, wherein the temperature controller is configured to adjust the temperature of a fluid within a sample chamber on the microarray such that the temperature of the fluid is varied during the analysis such that target DNA is dissociated from the microarray, and wherein the light source is directed to the microarray and the resulting fluorescence is perceived by the detector.
    Type: Grant
    Filed: June 25, 2009
    Date of Patent: August 11, 2015
    Assignee: Emergence Genomics, LLC
    Inventors: Michael Okura, Bruce J. Richardson
  • Patent number: 9023640
    Abstract: A portable system for real time detection of the presence of an infectious agent in a biological sample employs a reagent which detects the presence of a specific infectious agent in the sample, and emits a detectable signal when the reagent reacts with the sample and detects the presence of the infectious agent. A test cartridge has a reaction chamber for receiving the sample and the reagent. The reaction chamber has a predetermined internal geometry and at least one inner surface. Introducing the sample and the reagent into the test cartridge mixes the sample and the reagent. A testing unit receives the test cartridge, and includes a sensor for detecting an emitted detectable signal. The detection of the emitted detectable signal is indicative of the presence of the infectious agent in the sample.
    Type: Grant
    Filed: December 11, 2012
    Date of Patent: May 5, 2015
    Assignee: Fundamental Solutions Corporation
    Inventors: Marvin R. Williams, Charles McBrairty, Daniel W. Pfautz, Thomas J. Zupancic, Lingchun Zeng, Andrew Weiman, Richard S. Brody, Joseph Kittle, Anthony Truscott, Robert Baranowski
  • Patent number: 9017610
    Abstract: Systems and methods analyzing body fluids such as blood and bone marrow are disclosed. The systems and methods may utilize an improved technique for applying a monolayer of cells to a slide to generate a substantially uniform distribution of cells on the slide. Additionally aspects of the invention also relate to systems and methods for utilizing multi color microscopy for improving the quality of images captured by a light receiving device.
    Type: Grant
    Filed: April 27, 2009
    Date of Patent: April 28, 2015
    Assignee: Roche Diagnostics Hematology, Inc.
    Inventors: James Winkelman, Milenko Tanasijevic, David Zahniser
  • Publication number: 20150111200
    Abstract: The present teachings provide for systems, and components thereof, for detecting and/or analyzing light. These systems can include, among others, optical reference standards utilizing luminophores, such as nanocrystals, for calibrating, validating, and/or monitoring light-detection systems, before, during, and/or after sample analysis.
    Type: Application
    Filed: September 29, 2014
    Publication date: April 23, 2015
    Inventors: J. Michael PHILLIPS, Kevin S. Bodner, Aldrich N. K. Lau, Mark F. Oldham, Donald R. Sandell, David H. Tracy, Steven J. Boege
  • Patent number: 8993241
    Abstract: Provided is a device and a method whereby plural kinds of reaction operations and washing operations can be conducted in parallel without washing or replacing an instrument used in transferring a solution in each operation. A reaction device having a plurality of projecting barriers provided in a line on one surface of a substrate. The projecting barrier has a cutoff portion and an inner space capable of holding a droplet and having a contact angle to pure water of from 90 to 150 degrees. A reaction method using the reaction device wherein a substance immobilized on magnetic beads is sequentially transferred in and between droplets of a solution containing a surface tension reducing agent that are held in the spaces for holding a droplet by means of a magnet located on the opposite surface of the substrate to thereby conduct reactions and washings.
    Type: Grant
    Filed: January 16, 2009
    Date of Patent: March 31, 2015
    Assignee: National University Corporation University of Toyama
    Inventors: Masaharu Isobe, Nobuyuki Kurosawa
  • Patent number: 8993311
    Abstract: A disposable cartridge is described which is equipped with a plurality of microfabricated particle sorting structures. The disposable cartridge may include passageways which connect fluid reservoirs in the cartridge with corresponding microfluidic passageways on the particle sorting structure. A flexible gasket may prevent leakages and allow the fluid to cross the gasket barrier through a plurality of holes in the gasket, allowing fluid to be transferred from the reservoirs to the microfabricated particle sorting structures. The plurality of particle sorting structures may be arranged in the disposable cartridge in order to perform multiple separation operations, such as a sequential or parallel sorting operation.
    Type: Grant
    Filed: May 23, 2012
    Date of Patent: March 31, 2015
    Assignee: Innovative Micro Technology
    Inventors: John S. Foster, Daryl W. Grummitt, John C. Harley, James P. Linton, Jaquelin K. Spong, Douglas L. Thompson
  • Publication number: 20150087011
    Abstract: A device for analysis and monitoring of toxicity in waters is described. The device is specifically devised for determining toxicity in waters in several samples at the same time and in quick time with a high degree of accuracy and precision.
    Type: Application
    Filed: May 14, 2013
    Publication date: March 26, 2015
    Inventor: Pompeo Moscetta
  • Publication number: 20150064776
    Abstract: Provided is a sample dish for detecting microbes. The sample dish includes a base having an opening extending therethrough, an outer side wall at the outer periphery of the base, and an inner side wall at the inner periphery of the base defined by the opening. The sample dish may be used in conjunction with a microbial test unit including a housing defining an internal cavity configured to contain a sample dish, the unit including a cuff extending from an outlet of the housing into the internal cavity. The cuff may be an open-ended member configured to extend through an opening of the sample dish and provide fluid communication between the internal cavity and the outlet. The microbial test unit provides the ability to test compressed gas directly input to the unit.
    Type: Application
    Filed: August 19, 2014
    Publication date: March 5, 2015
    Inventors: John D'Antonio, H. Lee Scott
  • Patent number: 8968674
    Abstract: Provided herein is a fluid sensor, which includes a closed reaction unit in which reaction of a fluid sample takes place. The reaction unit is tapered on a side through which the fluid is injected so as to prevent generation of air bubbles during the injection of the fluid. Thus, the sensor has improved sensitivity.
    Type: Grant
    Filed: March 1, 2011
    Date of Patent: March 3, 2015
    Assignee: Samsung Electronics Co., Ltd.
    Inventors: Hun Joo Lee, Soo Suk Lee, Jung Nam Lee
  • Patent number: 8962341
    Abstract: An assay system designed to detect a protein biomarker in urine that is diagnostic for interstitial cystitis (IC). The presence of a 9 amino acid glycopeptide, antiproliferative factor (APF), in urine is unique to patients with IC. Urine samples from patients who exhibit symptoms consistent with IC are added to the assay system. Binding of APF to the cytoskeletal associated protein 4 (CKAP4) is positive for the presence of APF in urine and diagnostic for IC. The diagnostic system is a significant and surprising advance in diagnosis of IC and has commercial applications relevant to women and men who suffer from symptoms consistent with IC.
    Type: Grant
    Filed: December 10, 2009
    Date of Patent: February 24, 2015
    Assignees: The Commonwealth Medical College, University of Florida Research Foundation, Inc.
    Inventors: David Alan Zacharias, Sonia Lobo Planey
  • Patent number: 8962258
    Abstract: Provided are a multiple immunoassay apparatus on a chip in which a structure comprising multiple microfluidic channels is associated with a tissue sample, which allows immunohistochemical reactions to be conducted therein, to examine various markers specific for certain diseases, and a method for performing multiple immunoassays using the same. The multiple immunoassay apparatus comprises: at least one antibody-introducing unit through which at least one antibody is introduced into the apparatus; at least one reaction unit in which the antibody reacts with a sample in an immunohistochemical pattern; and at least one fluid outlet through which a fluid including the antibody is discharged outside the apparatus.
    Type: Grant
    Filed: August 29, 2008
    Date of Patent: February 24, 2015
    Assignees: Korea Advanced Institute of Science and Technology, National Cancer Center
    Inventors: Je-Kyun Park, MinSeok Kim, Eun Sook Lee, Sun-Young Kong, Solm Kwon
  • Publication number: 20150050684
    Abstract: The present invention relates to an apparatus that uses shadow images of cells to continuously measure cell activity at a high processing rate in order to provide cell activity and cell number results. According to one embodiment of the present invention, instead of a highly experienced examiner or technician using a microscope, ELISA reader, etc. having to collect various cell activity measurements and cell numbers, the collection of said information can be automated so as to reduce cost and largely reduce errors in measurements through the development of computer software coupled with hardware using low cost and compact optoelectronic components and simple image processing techniques.
    Type: Application
    Filed: December 27, 2012
    Publication date: February 19, 2015
    Inventors: Sung-Kyu Seo, Geon-Soo Jin, Un-Hwan Ha, Se-Hwan Paek, Seung-Pil Pack
  • Patent number: 8956861
    Abstract: Provided are a container tray in which a position and orientation of a container is hardly displaced from a predetermined position and orientation; a tray base used together with the container tray; and an observation unit. The container tray 8 includes a mounting plate 81 having a mounting surface 811 on which the container is to be mounted, an elastic body 82, and a biasing mechanism 83, 842. The elastic body 82 is arranged on the mounting surface 811 of the mounting plate 81 around a mounting region R where the container is to be mounted. The biasing mechanism 83, 842 is capable of switching states between a biased state in which the elastic body 82 is biased inward by applying a pressing force to the elastic body 82 from outside and a bias released state in which the bias on the elastic body 82 is released.
    Type: Grant
    Filed: September 14, 2012
    Date of Patent: February 17, 2015
    Assignee: Panasonic Healthcare Holdings Co., Ltd.
    Inventor: Mikio Houjou
  • Publication number: 20150044762
    Abstract: The present invention provides a device for detecting aerobic bacteria, anaerobic bacteria and fungi in a targeted material which comprises of a plurality sided slide, and a vial, wherein a first side of said plurality sided slide is coated with a first predetermined medium for aerobic bacteria growth and a second side of said plurality sided slide is coated with a second predetermined medium for fungal (mold and yeast) growth, and wherein at the bottom of said vial a third predetermined medium is embedded to determine a level of presence of anaerobic bacteria.
    Type: Application
    Filed: October 26, 2014
    Publication date: February 12, 2015
    Inventor: Saeed Mir Heidari