Abstract: Methods of in vitro propagation of plants of the genus Eriodictyon are described, including, in particular embodiments, plants of the species E. californicum, E. trichocalyx and E. sessilifolium. Methods of producing transgenic plants of the genus Eriodictyon are also described, along with methods of producing recombinant proteins in such plants. Compositions and methods for administering recombinant proteins produced in these plants are also described.

Abstract: A novel garden bean cultivar, designated H25101, is disclosed. The invention relates to the seeds of garden bean cultivar H25101, to the plants of garden bean line H25101 and to methods for producing a bean plant by crossing the cultivar H25101 with itself or another bean line. The invention further relates to methods for producing a bean plant containing in its genetic material one or more transgenes and to the transgenic plants produced by that method and to methods for producing other garden bean lines derived from the cultivar H25101.

Abstract: The invention provides seed and plants of the pea line designated 08530757. The invention thus relates to the plants, seeds and tissue cultures of pea line 08530757, and to methods for producing a pea plant produced by crossing a plant of pea line 08530757 with itself or with another pea plant, such as a plant of another line. The invention further relates to seeds and plants produced by such crossing. The invention further relates to parts of a plant of pea line 08530757, including the seed, pod, and gametes of such plants.

Abstract: The present invention relates to the production of novel, recombinant polynucleotides comprising the GIR1 ribozyme, or a variant thereof, vectors comprising such polynucleotides and recombinant host cells comprising such polynucleotides and/or such vectors. The invention furthermore relates to the use of said polynucleotides in the treatment of an individual suffering from a disease associated with or caused by instability of a transcript of said second subsequence such as cancer, cachexia, ?-Thallasemia or leukaemia.

Abstract: The present invention relates to the field of plant secondary metabolites. More particularly plant cell lines are established from the medicinal plant Veratrum californicum. Said plant cell lines can be used for the production of cyclopamine and related steroid alkaloids and their precursors.

Abstract: The present invention relates to the manipulation of the nitrogen metabolism in photosynthetic active organisms, preferably in plants. In particular, the present invention relates to a process for the enhanced nitrogen assimilation, accumulation and/or utilization and/or for the increased total nitrogen content in a photosynthetic active organism.

Abstract: The invention provides seed and plants of the pea line designated 08560865. The invention thus relates to the plants, seeds and tissue cultures of pea line 08560865, and to methods for producing a pea plant produced by crossing a plant of pea line 08560865 with itself or with another pea plant, such as a plant of another line. The invention further relates to seeds and plants produced by such crossing. The invention further relates to parts of a plant of pea line 08560865, including the seed, pod, and gametes of such plants.

Abstract: The invention provides seed and plants of the dry bean line designated 08520700. The invention thus relates to the plants, seeds and tissue cultures of dry bean line 08520700, and to methods for producing a dry bean plant produced by crossing a plant of dry bean line 08520700 with itself or with another dry bean plant, such as a plant of another line. The invention further relates to seeds and plants produced by such crossing. The invention further relates to parts of a plant of dry bean line 08520700, including the pods and gametes of such plants.

Abstract: A process for the production and maintenance of pluripotent and/or totipotent progenitor cereal cells from undifferentiated callus cells is described. Production of the progenitor cells takes place via direct organogenesis on a medium containing at least one auxin and at least one cytokinin. For example, the auxin may be 2,4-dichlorophenoxyacetic acid, indole-3-acetic acid, picloran, naphthelenacetic acid, indole-3-propionic acid, indole-3-butyric acid, phenyl acetic acid, benzofuran-3-acetic acid or phenyl butyric acid, and the cytokinin may be benzyl amino purine, benzyladenine, thidiazuron, zeatin, isopentyladenine, trans-zeatin or dimethylallyladenine. Processes for transformation of the undifferentiated callus cells and/or the progenitor cereal cells are also described. Typical cereal cells are sorghum, maize, wheat, barley, millet, rye, canola, alfalfa, triticale and rice.

Abstract: The invention provides seed and plants of the pea line designated 08540793. The invention thus relates to the plants, seeds and tissue cultures of pea line 08540793, and to methods for producing a pea plant produced by crossing a plant of pea line 08540793 with itself or with another pea plant, such as a plant of another line. The invention further relates to seeds and plants produced by such crossing. The invention further relates to parts of a plant of pea line 08540793, including the seed, pod, and gametes of such plants.

Abstract: The present invention relates to a novel perfusion bioreactor allowing continuous medium feed and extraction of metabolites or other desired products from cells. The invention is useful for plant cell cultures but may also be used for mammalian cell cultures, insect cell cultures and bacterial cell cultures. The design of the reactor includes sedimentation columns mounted inside the bioreactor to separate single cells and cell aggregates from the culture medium at a very low shear stress. The operating conditions allow a stable cell/medium separation by maintaining the medium upward velocity equal to or slightly lower than the cell sedimentation velocity.

Abstract: The invention provides seed and plants of the dry bean line designated 08550813. The invention thus relates to the plants, seeds and tissue cultures of dry bean line 08550813, and to methods for producing a dry bean plant produced by crossing a plant of dry bean line 08550813 with itself or with another dry bean plant, such as a plant of another line. The invention further relates to seeds and plants produced by such crossing. The invention further relates to parts of a plant of dry bean line 08550813, including the pods and gametes of such plants.

Abstract: The invention provides seed and plants of the dry bean line designated 08530714. The invention thus relates to the plants, seeds and tissue cultures of dry bean line 08530714, and to methods for producing a dry bean plant produced by crossing a plant of dry bean line 08530714 with itself or with another dry bean plant, such as a plant of another line. The invention further relates to seeds and plants produced by such crossing. The invention further relates to parts of a plant of dry bean line 08530714, including the pods and gametes of such plants.

Abstract: The invention provides seed and plants of the dry bean line designated 08540800. The invention thus relates to the plants, seeds and tissue cultures of dry bean line 08540800, and to methods for producing a dry bean plant produced by crossing a plant of dry bean line 08540800 with itself or with another dry bean plant, such as a plant of another line. The invention further relates to seeds and plants produced by such crossing. The invention further relates to parts of a plant of dry bean line 08540800, including the pods and gametes of such plants.

Abstract: The invention provides seed and plants of pepper varieties designated 9954288, SBR 99-1274 and SBY 99-1273. The invention thus relates to the plants, seeds and tissue cultures of sweet pepper hybrid 9954288, pepper line SBR 99-1274 and pepper line SBY 99-1273, and to methods for producing a pepper plant produced by crossing a plant with itself or with another pepper plant, such as a plant of another line. The invention further relates to seeds and plants produced by such crossing. The invention further relates to parts of a plant of the invention, including the fruit and gametes of such plants.

Abstract: Contamination by microorganism of an aqueous proline solution is prevented by adjusting a proline concentration of the solution to 20% (W/W/) or more. Such solutions are useful as fertilizers and/or plan vitalizers. Preservation of the novel solutions are possible over a significant period of time, allowing for more flexibility in the distribution and application of commercial products made from the solutions.

Abstract: The present invention provides a novel method for culturing cells as well as a novel method for producing a recombinant protein by culturing cells at large scale (up to 1,500 L nominal volume and 750 L working volume), whereby an inflated bag provides a sterile, disposable cultivation chamber. The inflated bag is partially filled with liquid cultivation media and cells, and placed into a containment vessel. The containment vessel is positioned onto an orbitally shaken platform. The orbital shaking moves the containment vessel and thus the bag and induces thereby motion to the liquid contained therein (“shake mixing”). This motion (caused by orbital shaking) induces a dynamic force field that ensures cell suspension, bulk mixing, and oxygen transfer from the liquid surface to the respiring cells without damaging shear or foam generation.

Abstract: The present invention relates to binding proteins and, in particular, humanized antibodies that may be used, for example, in the diagnosis, treatment and prevention of Alzheimer's Disease and related conditions.

Abstract: This invention relates to polynucleotide sequences encoding a gene that can confer resistance to at least one herbicide. It further relates to plants and seeds of plants carrying chimeric genes comprising said polynucleotide sequences, which enhance or confer resistance to at least one herbicide, and methods of making said plants and seeds. The invention further presents sequences that can be used as molecular markers that in turn can be used to identify the region of interest in corn lines resulting from new crosses and to quickly and efficiently select the best lines for breeding strategies by avoiding sensitive lines.

Abstract: This invention provides cylindrical cell culture tubes with a cap having both a septum and gas exchange membranes. The culture tubes can be used to inoculate media, culture cells, harvest cells and store cells in the same container with reduced risk of contamination, while facilitating automated handling.

Abstract: The present invention provides methods and devices for the rapid isolation of monocot plant embryos suitable for transformation or tissue culture. The invention includes mechanical devices for substantially isolating plant embryos for use as transformable explants. Media suitable for isolating plant embryos and methods for their preparation are also provided.

Abstract: Systems and methods for hydroponically growing microorganisms within a self-contained air-supported structure, in which microorganisms are grown in an organic slurry, harvested, and processed to obtain and process and distribute molecules useful for biofuel or other purposes.

Abstract: Described herein is the isolation and identification of a number of sulfur oxidizing plant growth promoting rhizobacteria: RAY12, identified as Achromobacter piechaudii; RAY28, identified as Agrobacterium tumefaciens, RAY132, identified as Stenotrophomonas maltophilia; and RAY209, identified as Delftia acidovorans. The PGPR act to oxidize elemental sulfur which in turn provides sulfate for the plants. As a result of this arrangement, plants are able to grow more efficiently and effectively and have enhanced growth characteristics, for example, but by no means limited to, increased vigor, early emergence, increased emergence rate, increased biomass, increased plant leaf area, higher crop yield, increased pod number, increased pod weight, increased root biomass, increased seed weight, increased macro- and micro-nutrient uptake and the like. The sulfur-oxidizing PGPR may be applied to seeds, seed pieces, carrier materials, roots and planting soil.

Abstract: Described herein is the isolation and identification of a number of sulfur oxidizing plant growth promoting rhizobacteria: RAY12, identified as Achromobacter piechaudii; RAY28, identified as Agrobacterium tumefaciens, RAY132, identified as Stenotrophomonas maltophilia; and RAY209, identified as Delftia acidovorans. The PGPR act to oxidize elemental sulfur which in turn provides sulfate for the plants. As a result of this arrangement, plants are able to grow more efficiently and effectively and have enhanced growth characteristics, for example, but by no means limited to, increased vigor, early emergence, increased emergence rate, increased biomass, increased plant leaf area, higher crop yield, increased pod number, increased pod weight, increased root biomass, increased seed weight, increased macro- and micro-nutrient uptake and the like. The sulfur-oxidizing PGPR may be applied to seeds, seed pieces, carrier materials, roots and planting soil.

Abstract: The present invention relates to improved methods for the incorporation of DNA into the genome of a wheat plant based on a D-alanine or D-serine selection. Preferably, the transformation is mediated by Agrobacterium.

Abstract: The present invention provides a method and a culture medium for the production of food biomass by directly culturing seed kernel tissue, or seed cotyledonary differentiated tissue. The culture medium of the present invention contains at least DKW culture medium, Vitamin MS culture medium with an enriched concentration of thiamine, sacarose, kinetine, adenine, 2,4diclorofenoxiacético (2,4D), L-Glutamine, cysteine, ascorbic acid, and Gelrite®.

Abstract: The present invention provides a method and a culture medium for the production of food biomass by directly culturing seed kernel tissue, or seed cotyledonary differentiated tissue. The culture medium of the present invention contains at least DKW culture medium, Vitamin MS culture medium with an enriched concentration of thiamine, sacarose, kinetine, adenine, 2,4diclorofenoxiacético (2,4D), L-Glutamine, cysteine, ascorbic acid, and Gelrite®.

Abstract: Described herein is the isolation and identification of a number of sulfur oxidizing plant growth promoting rhizobacteria: RAY12, identified as Achromobacter piechaudii; RAY28, identified as Agrobacterium tumefaciens, RAY132, identified as Stenotrophomonas maltophilia; and RAY209, identified as Delftia acidovorans. The PGPR act to oxidize elemental sulfur which in turn provides sulfate for the plants. As a result of this arrangement, plants are able to grow more efficiently and effectively and have enhanced growth characteristics, for example, but by no means limited to, increased vigor, early emergence, increased emergence rate, increased biomass, increased plant leaf area, higher crop yield, increased pod number, increased pod weight, increased root biomass, increased seed weight, increased macro- and micro-nutrient uptake and the like. The sulfur-oxidizing PGPR may be applied to seeds, seed pieces, carrier materials, roots and planting soil.

Abstract: Described herein is the isolation and identification of a number of sulfur oxidizing plant growth promoting rhizobacteria: RAY12, identified as Achromobacter piechaudii; RAY28, identified as Agrobacterium tumefaciens, RAY132, identified as Stenotrophomonas maltophilia; and RAY209, identified as Delftia acidovorans. The PGPR act to oxidize elemental sulfur which in turn provides sulfate for the plants. As a result of this arrangement, plants are able to grow more efficiently and effectively and have enhanced growth characteristics, for example, but by no means limited to, increased vigor, early emergence, increased emergence rate, increased biomass, increased plant leaf area, higher crop yield, increased pod number, increased pod weight, increased root biomass, increased seed weight, increased macro- and micro-nutrient uptake and the like. The sulfur-oxidizing PGPR may be applied to seeds, seed pieces, carrier materials, roots and planting soil.

Abstract: Described herein is the isolation and identification of a number of sulfur oxidizing plant growth promoting rhizobacteria: RAY12, identified as Achromobacter piechaudii; RAY28, identified as Agrobacterium tumefaciens, RAY132, identified as Stenotrophomonas maltophilia; and RAY209, identified as Delftia acidovorans. The PGPR act to oxidize elemental sulfur which in turn provides sulfate for the plants. As a result of this arrangement, plants are able to grow more efficiently and effectively and have enhanced growth characteristics, for example, but by no means limited to, increased vigor, early emergence, increased emergence rate, increased biomass, increased plant leaf area, higher crop yield, increased pod number, increased pod weight, increased root biomass, increased seed weight, increased macro- and micro-nutrient uptake and the like. The sulfur-oxidizing PGPR may be applied to seeds, seed pieces, carrier materials, roots and planting soil.

Abstract: A device, system and method for axenically culturing and harvesting cells and/or tissues, including bioreactors and fermentors. The device is preferably disposable but nevertheless may be used continuously for a plurality of consecutive culturing/harvesting cycles prior to disposal of same. This invention also relates to batteries of such devices which may be used for large-scale production of cells and tissues. According to preferred embodiments of the present invention, the present invention is adapted for use with plant cell culture.

Abstract: Described herein is the isolation and identification of a number of sulfur oxidizing plant growth promoting rhizobacteria: RAY12, identified as Achromobacter piechaudii; RAY28, identified as Agrobacterium tumefaciens, RAY132, identified as Stenotrophomonas maltophilia; and RAY209, identified as Delftia acidovorans. The PGPR act to oxidize elemental sulfur which in turn provides sulfate for the plants. As a result of this arrangement, plants are able to grow more efficiently and effectively and have enhanced growth characteristics, for example, but by no means limited to, increased vigor, early emergence, increased emergence rate, increased biomass, increased plant leaf area, higher crop yield, increased pod number, increased pod weight, increased root biomass, increased seed weight, increased macro- and micro-nutrient uptake and the like. The sulfur-oxidizing PGPR may be applied to seeds, seed pieces, carrier materials, roots and planting soil.

Abstract: The present invention relates to the commercially viable process for in-vitro mass culture of Chlorophytum borivilianum. The present invention process for in-vitro mass culture of Chlorophytum borivilianum is simple, faster and suitable for production of disease free root tubers of uniform quality. The process of the present invention for in-vitro mass culture of Chlorophytum borivilianum employs media with low concentration of nutrients and phytohormones.

Abstract: The subject invention relates to recovery of cryopreserved plant cell cultures after cryopreservation. The use of canine IL-4 and human gamma interferon is exemplified in some preferred embodiments.

Abstract: The present invention relates to the production of proteins in a cell or host cell. The invention uses a TRAnscription Pause (TRAP) sequence to enhance a protein expression characteristic of a protein expression unit. The TRAP sequence is thought to prevent, at least in part, formation of antisense RNA or to, at least in part, prevent transcription to enter the protein expression unit. In one embodiment, the invention provides a method for expression of at least one protein of interest in a cell comprising providing the cell with at least one protein expression unit that comprises a promoter functionally linked to an open reading frame encoding at least one protein of interest, characterized in that the protein expression unit further comprises at least one TRAP sequence and wherein the TRAP sequence is functionally located downstream of the open reading frame and at least in part prevents formation of antisense RNA.

Abstract: This invention is a method of minimizing the variation of cell growth and production through homogeneous cell line development. To be more specific, it is the method of isolating and proliferating single cell clone from the procambium to promote the stability of the plant-derived biologically active substances production by solving the problems of decrease in cell growth and the productivity during the long term culture.

Abstract: A method of ex-vivo expanding a population of stem cells, while at the same time inhibiting differentiation of the stem cells.

Abstract: Methods are taught for culturing mammalian cells, preferably human cells, to improve production of proteins, recombinant or endogenous. Methods are also provided for the growth and long-term survival of cell lines, particularly cell lines established from primary culture. Cell culture media are also provided, contained varying levels of selected amino acids, supplemented with various growth factors and trace elements. In additionally, the media are optionally serum-free, and preferably use an energy source other than glucose. The media are particularly suitable for the primary culture and long-term culture of human fetal cells.

Abstract: The present invention relates to excision of explant material comprising meristematic tissue from seeds, and storage of such material prior to subsequent use in plant tissue culture and genetic transformation. Methods for tissue preparation, storage, and transformation are disclosed, as is transformable meristem tissue produced by such methods, and apparati for tissue preparation.

Abstract: The present invention provides a method of micropropagating a monocotyledonous plant comprising: (a) cultivating an explant of tissue from a monocotyledonous plant shoot tip on a primary medium, wherein the explant has been pretreated with a cold temperature and the primary medium comprises auxin or auxin and cytokinin, to produce a totipotent embryogenic cell culture; (b) treating the totipotent embryonic cell culture with a cold temperature; (c) maintaining the totipotent embryogenic cell culture by cultivation on a secondary medium, whereby a totipotent embryogenic cell culture of a monocotyledonous plant is produced and maintained; and (d) transferring the embryogenic cell culture of step (c) to a tertiary medium to continue multiplication and to produce a plantlet with roots and shoots, thereby micropropagating a monocotyledonous plant. The micropropagation techniques described herein provide plants for such purposes as development of elite plant lines, phytoremediation and biomass production.

Abstract: Solanum viarum is an alkaloid producing plant of the family Solanaceae with varied therapeutic uses. In order to grow the plant in large areas; one needs to have an efficient system of vegetative multiplication, which ensures its genetic uniformity, and true to the type nature. In nature largely seeds propagate plant, which could be result of cross-pollination which may result in genetic drift. The present invention provides an efficient micropropagation system, with high level of multiplication at relatively low cost of production. The multiplication ratio was as high as 1:6 and almost 95% the plants were viable and successfully cultivated in field. The present invention provides an ideal way of mass cultivation of the selected elite plant material.

Abstract: It is intended to provide a pure line plant which is required in constructing a seed propagation variety of orchid. An auxin solution is dropped to unfertilized orchid flowers so as to form seeds based on parthenogenesis. Then these seeds are germinated and haploid plants are selected from orchid plants thus grown. The germinating seeds judges as haploid plants are grown to give pure line plants having doubled chromosomes. Thus, a seed propagation variety of orchid is obtained.

Abstract: A tissue culture micropropagation process for Hoodia plants, which achieves new shoots in the multiplication stage of the process by using an inventive combination of phytohormones.

Abstract: An in vitro tissue culture process of rooting Hoodia explants by using an inventive combination of phytohormones.

Abstract: The present invention relates to a petunia plant, seed, variety and hybrid. More specifically, the invention relates to a petunia plant having an allele which results in a petunia plant with altered flower color and/or altered flower color pattern. The invention also relates to crossing petunia plants containing the allele with petunia or Calibrachoa plants lacking the allele to produce novel types of petunia and Calibrachoa-petunia inter-generic hybrid plants.

Abstract: This invention is a method of minimizing the variation of cell growth and production through homogeneous cell line development. To be more specific, it is the method of isolating and proliferating single cell clone from the procambium or cambium to promote the stability of the plant-derived biologically active substances production by solving the problems of decrease in cell growth and the productivity during the long term culture. Key words: Procambium or cambium, single cell clone, cell aggregation, dedifferentiation, cryoperservation, variability.

Abstract: A process of mechanical separation of embryos from seeds for genetic transplantation employs counter-rotating cylinders together with one or more culling, hydration, separation, and viability testing steps to provide high-throughput of viable, transplantable tissue.

Abstract: A substantially microbe-free composition containing plant growth regulators is produced by culturing a fungal spawn on high carbohydrate medium under suitable conditions. The culture filtrate is sterilized, formulated, and used to enhance plant growth.

Abstract: The present invention relates to an efficient method of in-vitro micropropagation of Piper longum plants. In particular, the present invention is directed towards the novel method for micropropagation of Piper longum from lateral bud (meristematic) explant (starting material), by culturing the explants on different medias. This method results in mass production of the plant Piper longum in a short span of time.

Abstract: The present invention provides an in vitro resveratrol-rich callus tissue of Vitis thunbergii Sieb. et Zucc. which is a callus tissue developed from a tissue culture system containing one or more plant growth regulators (PGRs) and cultured from a stem or a petiole tissue explant of a wild type of V. thunbergii or a cultivated plantlet of V. thunbergii. The cultivated plantlet is in turn derived from a shoot of the wild type of V. thunbergii cultivated in a plantlet culture system containing no PGRs. The in vitro resveratrol-rich callus tissue of V. thunbergii is characterized by its containing at least about 1,000 to 10,000 mg/kg of dried weight of resveratrol, predominantly in the form of trans-resveratrol and/or resveratrol-O-glucoside, and being ready for harvest or subculture in about 30 days.