Abstract: Provide are a peptide gel with practically sufficient mechanical strength and a self-assembling peptide capable of forming the peptide gel. The self-assembling peptide is formed of the following amino acid sequence: a1b1c1b2a2b3db4a3b5c2b6a4 where: a1 to a4 each represent a basic amino acid residue; b1 to b6 each represent an uncharged polar amino acid residue and/or a hydrophobic amino acid residue, provided that at least five thereof each represent a hydrophobic amino acid residue; c1 and c2 each represent an acidic amino acid residue; and d represents a hydrophobic amino acid residue.

Abstract: A gas-introduction system useful, for example, in biotechnology for supplying cells or microorganisms with oxygen. The gas-introduction system has a bubble column and a distributor. In the vessel there is situated a liquid medium which is to be supplied with gas. A vector is used as transport medium for the gas. The gas is introduced into the bubble column and here taken up by the vector. The vector is applied to the liquid surface via the distributor in droplet form, falls downward in the medium and releases to the medium some of the gas that has been taken up. At the bottom of the vessel is situated a collecting device in which the vector droplets coalesce and pass back into the bubble column. Disclosed are a bioreactor comprising the gas-introduction system and a method for introducing gas into a liquid medium, preferably an aqueous suspension containing cells or microorganisms.

Abstract: A method of producing a stably transformed corn plant in a single container is demonstrated. This method allows for the automation of the transformation process and reduces labor, material, and ergonomic costs associated with traditional plant tissue culture systems.

Abstract: A method for determining the effect of a plurality of culture conditions on a cell, comprising the steps of: a) providing a first set of groups of cell units each comprising one or more cells, and exposing said groups to desired culture conditions; (b) pooling two or more of said groups to form at least one second pool; (c) subdividing the second pool to create a further set of groups of cell units; (d) exposing said further groups to desired culture conditions; (e) optionally, repeating steps (b)-(d) iteratively as required; and (f) optionally assessing the effect on a given cell unit of the culture conditions to which it has been exposed.

Abstract: A flexible wall bioreactor is described that uses a small droplet size mist unit, a lower rate ambient air flow rate, and a flexible wall culture chamber to provide an environment that allows for the growth of dense root matrix, shoot cultures, and 2 and 3 dimensional animal tissues.

Abstract: The present invention provides a method of micropropagating a monocotyledonous plant comprising: (a) cultivating an explant of tissue from a monocotyledonous plant shoot tip on a primary medium, wherein the explant has been pretreated with a cold temperature and the primary medium comprises auxin or auxin and cytokinin, to produce a totipotent embryogenic cell culture; (b) treating the totipotent embryonic cell culture with a cold temperature; (c) maintaining the totipotent embryogenic cell culture by cultivation on a secondary medium, whereby a totipotent embryogenic cell culture of a monocotyledonous plant is produced and maintained; and (d) transferring the embryogenic cell culture of step (c) to a tertiary medium to continue multiplication and to produce a plantlet with roots and shoots, thereby micropropagating a monocotyledonous plant. The micropropagation techniques described herein provide plants for such purposes as development of elite plant lines, phytoremediation and biomass production.

Abstract: The present invention relates to a method of producing corosolic acid by using plant cells that produce corosolic acid. More particularly, the present invention relates to a method of producing corosolic acid by using plant cell suspension cultures comprising the steps of: inducing a callus from a tissue of a plant producing corosolic acid; preparing a cell line capable of being cultured in liquid culture medium from the induced callus; culturing the cell line in a suspension culture; and isolating corosolic acid from the culture solution. The present invention has advantage of maximizing productivity by utilizing two-stage culture, treatment with inducing agent, and high cell-density culture in the suspension culture of plant cells producing corosolic acid.

Abstract: This document discloses electroporation vessels, electrocompetent cells that have been aliquoted and frozen in electroporation vessels, and a number of other apparatuses, kits, and methods for electroporation. Some embodiments of electroporation vessels described herein may include a pair of opposing walls that are downwardly angled toward one another in a gap between two electrode surfaces. Further embodiments of devices and methods described herein may eliminate the need for an end user to transfer competent cells from a capped tube to electroporation cuvette, thereby saving time and producing less waste.

Abstract: Certain embodiments disclosed herein include, but are not limited to, at least one of compositions, methods, devices, systems, kits, or products regarding rejuvenation or preservation of germ cells or gametes. Certain embodiments disclosed herein include, but are not limited to, methods of modifying germ cells or gametes, or methods of administering modified germ cells or gametes to at least one biological tissue.

Abstract: The invention provides seed and plants of the pea line designated 08250838. The invention thus relates to the plants, seeds and tissue cultures of pea line 08250838, and to methods for producing a pea plant produced by crossing a plant of pea line 08250838 with itself or with another pea plant, such as a plant of another line. The invention further relates to seeds and plants produced by such crossing. The invention further relates to parts of a plant of pea line 08250838, including the seed, pod, and gametes of such plants.

Abstract: A novel garden bean cultivar, designated SB4372, is disclosed. The invention relates to the seeds of garden bean cultivar SB4372, to the plants of garden bean line SB4372 and to methods for producing a garden bean plant by crossing the cultivar SB4372 with itself or another garden bean line. The invention further relates to methods for producing a garden bean plant containing in its genetic material one or more transgenes and to the transgenic plants produced by that method and to methods for producing other garden bean lines derived from the cultivar SB4372.

Abstract: Disclosed herein are bioreactors including a first sheet and a second sheet (one or both of which is substantially transparent to light), wherein the second sheet is disposed adjacent to the first sheet, and the first and second sheets are sealed along a first longitudinal edge, a second longitudinal edge, a first horizontal edge, a second horizontal edge, and at least one intermediate horizontal seal between the first horizontal edge and the second horizontal edge, thereby forming at least two chambers for holding fluid in series along a vertical axis, wherein each of the two or more chambers is oriented at an angle relative to the vertical axis, wherein the angle is about 0° to about 90° and at least one of the chambers is oriented at an angle greater than 0°, and wherein there is at least one opening in each of the first horizontal edge, the second horizontal edge, and intermediate horizontal seal(s); a support structure comprising at least one horizontal support, wherein the horizontal support is located

Abstract: The invention provides seed and plants of the pea line designated 08240772. The invention thus relates to the plants, seeds and tissue cultures of pea line 08240772, and to methods for producing a pea plant produced by crossing a plant of pea line 08240772 with itself or with another pea plant, such as a plant of another line. The invention further relates to seeds and plants produced by such crossing. The invention further relates to parts of a plant of pea line 08240772, including the seed, pod, and gametes of such plants.

Abstract: A novel garden bean cultivar, designated SB4348, is disclosed. The invention relates to the seeds of garden bean cultivar SB4348, to the plants of garden bean line SB4348 and to methods for producing a garden bean plant by crossing the cultivar SB4348 with itself or another garden bean line. The invention further relates to methods for producing a garden bean plant containing in its genetic material one or more transgenes and to the transgenic plants produced by that method and to methods for producing other garden bean lines derived from the cultivar SB4348.

Abstract: A compact, high-yield, bio-reactor for use as small, medium and large-scale production unit for therapeutic proteins, proteins in general and enzymes based on controlled activity of the cells or micro organisms and the production of such as high value antibodies for pharmaceutical and/or bio-diagnostic applications.

Abstract: The invention relates to a bioreactor, to the use of the bioreactor for culturing microorganisms or cell cultures, and also to a method for culturing microorganisms or cell cultures.

Abstract: The invention provides seed and plants of the pea line designated 08250833. The invention thus relates to the plants, seeds and tissue cultures of pea line 08250833, and to methods for producing a pea plant produced by crossing a plant of pea line 08250833 with itself or with another pea plant, such as a plant of another line. The invention further relates to seeds and plants produced by such crossing. The invention further relates to parts of a plant of pea line 08250833, including the seed, pod, and gametes of such plants.

Abstract: The present invention provides a method for improving the preparation and use of growth media by the uses of specific pellet formulations, which especially are tablets of different sizes, which contain the growth medium or parts thereof and are sterilized with the standard methods of pharmaceutical technology. Specifically these pellet formulations are applied to control a cell culture in a way that the adaptation phase is shorter or that the growth is controlled by a release of certain components at a certain time and in a certain concentration during the process, and nutrients (e.g., nitrogen) can be packed into the cultivation vessel in amounts sufficient for high cell densities without the risk of intoxication of the organism.

Abstract: A process to prepare a recombined transgenic Zea mays plant or plant cell from a first transgenic Zea mays plant cell, wherein the transgene in the recombinant plant or plant cell has an altered genetic structure relative to the genetic structure of the transgene in the first transgenic plant cell, due to homologous recombination-mediated transgene deletion.

Abstract: The present invention provides a method of micropropagating a monocotyledonous plant comprising: (a) cultivating an explant of tissue from a monocotyledonous plant shoot tip on a primary medium, wherein the explant has been pretreated with a cold temperature and the primary medium comprises auxin or auxin and cytokinin, to produce a totipotent embryogenic cell culture; (b) treating the totipotent embryonic cell culture with a cold temperature; (c) maintaining the totipotent embryogenic cell culture by cultivation on a secondary medium, whereby a totipotent embryogenic cell culture of a monocotyledonous plant is produced and maintained; and (d) transferring the embryogenic cell culture of step (c) to a tertiary medium to continue multiplication and to produce a plantlet with roots and shoots, thereby micropropagating a monocotyledonous plant. The micropropagation techniques described herein provide plants for such purposes as development of elite plant lines, phytoremediation and biomass production.

Abstract: Disclosed herein is mesoscale bioreactor platform comprising an upwards open chamber for a biological cell, which chamber via a first port is in communication with a first channel for conducting an influent stream of a liquid into the chamber and via a second port is in communication with a second channel for conducting an effluent stream of a liquid away from the chamber, which chamber is provided with a closure comprising a water-immiscible liquid, and wherein said first channel is in fluid communication with a reservoir for a liquid and said second channel is in fluid communication with a waste container. Furthermore, a method for modifying the interaction of a content of a chamber with the surroundings is described as well as method of culturing a biological cell.

Abstract: A method for promoting maturation and development of vigorous conifer (gymnosperm) somatic embryos comprising the use of S(+)-ABA as the substantive form of ABA.

Abstract: The present invention provides methods for regrowth of conifer embryogenic cells. The methods comprise the steps of (a) contacting cryopreserved conifer embryogenic cells with a liquid transition medium, and (b) culturing the contacted conifer embryogenic cells in a regrowth medium to generate regrowth of the conifer embryogenic cells. Generally, the cryopreserved conifer embryogenic cells are contacted with the liquid transition medium for less than about 24 hours, such as for about one hour.

Abstract: The subject invention provides simple and consistent methods to break suspension cell aggregates to single cells with intact primary cell walls. The subject invention relates in part to cell separation of suspension cell aggregates cultured in medium containing pectin-degrading enzymes or tubulin de-polymerizing compounds including colchicine. The subject invention also relates to novel uses of compounds for such purposes. Another aspect of the subject invention relates to transformation of the subject, isolated cells. Such processes simplify and integrate single-cell-based transformation and selection processes into transgenic and transplastomic event-generation work processes. The subject invention also removes technical constraints and produces marker-free and uniformly expressing transgenic lines in a high throughput fashion to support various needs of animal health, biopharma, and trait and crop protection platforms.

Abstract: The present invention provides methods and devices for the rapid isolation of monocot plant embryos suitable for transformation or tissue culture. The invention includes mechanical devices for substantially isolating plant embryos for use as transformable explants. Media suitable for isolating plant embryos and methods for their preparation are also provided.

Abstract: The invention provides seed and plants of the bean line designated 08550815. The invention thus relates to the plants, seeds and tissue cultures of bean line 08550815, and to methods for producing a bean plant produced by crossing a plant of bean line 08550815 with itself or with another bean plant, such as a plant of another line. The invention further relates to seeds and plants produced by such crossing. The invention further relates to parts of a plant of bean line 08550815, including the pods and gametes of such plants.

Abstract: A system for mixing a liquid solution or suspension includes a support housing bounding a compartment and a collapsible bag disposed within the compartment. A mixer is disposed within the collapsible bag while a sparger delivers a gas to the lower end of the collapsible bag. A gas outlet line extends from an upper end of the collapsible bag to a condenser assembly. A gas exhaust line and a fluid collection line both extend from the condenser assembly.

Abstract: A novel garden bean cultivar, designated SB4355, is disclosed. The invention relates to the seeds of garden bean cultivar SB4355, to the plants of garden bean line SB4355 and to methods for producing a garden bean plant by crossing the cultivar SB4355 with itself or another garden bean line. The invention further relates to methods for producing a garden bean plant containing in its genetic material one or more transgenes and to the transgenic plants produced by that method and to methods for producing other garden bean lines derived from the cultivar SB4355.

Abstract: The present invention provides a method for reducing or inhibiting fucose modification to a N-linked sugar chain in a plant, and a transgenic plant, or the like, in which the fucose modification has been reduced or inhibited. Transcription or translation of a gene coding for an enzyme involved in synthesis of sugar nucleotide GDP-fucose is inhibited by post-transcriptional gene silencing (PTGS), virus-induced gene silencing (VIGS) or transcriptional gene silencing (TGS), and an amount of GDP-fucose in a plant cell is reduced, to reduce or inhibit fucose modification to a sugar chain including glycoprotein sugar chain, glycolipid sugar chain, oligosaccharide, polysaccharide, and the like, thereby enabling production of a glycoprotein, or the like, from which fucose modification, which may become an allergen, has been removed, and enabling clinical application of a medicinal glycoprotein produced with a plant.

Abstract: The present disclosure relates to compositions, systems, and methods for preserving and/or stabilizing a cell (e.g., a whole cell). A cell and/or macromolecule stabilizing composition may include a chelator, a chelator enhancing component, and optionally a base (e.g., a purine base or a pyrimidine base). A cell stabilizing method may include contacting a cell with a cell and/or macromolecule stabilizing composition. A cell stabilizing system may include a container suitable for receiving a sample containing a cell and a cell and/or macromolecule stabilizing composition. A cell may be preserved and/or stabilized under ambient conditions (e.g., without refrigeration). A cell may include a protein, a nucleic acid, and/or another biomolecule marker of cell preservation and/or stabilization. A composition may be configured to preserve and/or stabilize one or more cells for analysis by flow cytometry and simultaneously preserve and/or stabilize one or more intracellular nucleic acids for molecular analysis.

Abstract: The invention relates to a process for reducing the amount of deposits during the culture of organisms, and in particular of cell cultures, where these have a tendency towards agglomeration or adhesion to the bioreactor and to its elements, or where these are readily susceptible to agglomeration or adhesion of cell debris or of substances.

Abstract: The invention relates to a process for increasing the amount of enzymes in a grain by germinating the grain in conditions in which the grain is wounded. The invention also relates to hydrolases obtainable from grains for use in food processing and manufacturing industries, to the germination of grains such as barley and to isolation of enzymes from grains.

Abstract: A device, system and method for axenically culturing and harvesting cells and/or tissues, including bioreactors and fermentors. The device is preferably disposable but nevertheless may be used continuously for a plurality of consecutive culturing/harvesting cycles prior to disposal of same. This invention also relates to batteries of such devices which may be used for large-scale production of cells and tissues. According to preferred embodiments of the present invention, the present invention is adapted for use with plant cell culture.

Abstract: The invention provides seed and plants of the pea line designated 08270930. The invention thus relates to the plants, seeds and tissue cultures of pea line 08270930, and to methods for producing a pea plant produced by crossing a plant of pea line 08270930 with itself or with another pea plant, such as a plant of another line. The invention further relates to seeds and plants produced by such crossing. The invention further relates to parts of a plant of pea line 08270930, including the seed, pod, and gametes of such plants.

Abstract: The present invention is a method for isolating a desired cell, which comprises selectively applying culture conditions including a culture medium to a sample potentially containing various cells, with addition of a cell enlarger simultaneously with, before or after the application. Thereby, it is possible to conveniently and efficiently obtain unknown but useful microorganisms occurring in a natural environment that are less competitive.

Abstract: The present invention relates to nutritive medium, medium supplement, media subgroup and buffer formulations. The present invention provides powder nutritive medium, medium supplement and medium subgroup formulations, e.g., cell culture medium supplements (including powdered sera such as powdered fetal bovine serum (FBS)), medium subgroup formulations and cell culture media comprising all of the necessary nutritive factors that facilitate the in vitro cultivation of cells. The invention further provides powder buffer formulations that produce particular ionic and pH conditions upon reconstitution with a solvent. The invention provides methods for production of media, media supplement, media subgroup and buffer formulations, and also provides kits and methods for cultivation of prokaryotic and eukaryotic cells, particularly bacterial cells, yeast cells, plant cells and animal cells (including human cells) using these dry powder nutritive media, media supplement, media subgroup and buffer formulations.

Abstract: The present invention relates to isolated polypeptides having alpha-L-arabinofuranosidase activity and isolated polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides.

Abstract: A method for promoting maturation and development of vigorous conifer (gymnosperm) somatic embryos comprising the use of S(+)-ABA as the substantive form of ABA.

Abstract: In one aspect, the present invention provides methods of producing a synchronized population of conifer somatic embryo germinants in preparation for transplantation. The methods of the invention comprise the steps of: (a) culturing conifer somatic embryos on sterile germination medium for a sufficient period of time to produce germinants comprising a visible, well-defined epicotyl stem of a desired length; (b) selecting a plurality of germinants having an epicotyl stem of the desired length; (c) storing the selected germinants having an epicotyl stem of the desired length produced in step (a) while still on the sterile germination medium from step (a) in a cold environment in which the temperature is in the range of 0.5° C. to 10° C. for a time period up to six months; and (d) transferring the germinants stored according to step (c) from the germination medium into water for a time of up to about four weeks at a temperature of about 0.5° C. to about 5° C.

Abstract: The present invention relates to an antioxidant, anti-inflammatory or anti-aging composition containing any one or more of a Taxus cambium- or procambium-derived cell line, an extract thereof, a lysate thereof and a culture medium thereof. The composition according to the present invention has minimized side effects compared to existing antioxidants and anti-inflammatory agents, is involved in intracellular metabolism to reduce intracellular reactive oxygen species, and reduces and induces aging-related signals. Thus, the composition of the preset invention is useful for preventing and delaying aging. In addition, the composition of the present invention has the effect of inhibiting melanogenesis, and thus is useful as a whitening cosmetic composition.

Abstract: The invention provides Brassica lines having an average Sclerotinia sclerotiorum Disease Incidence (SSDI %) score which is less than about 60% of the SSDI % score of appropriate check varieties. Methodologies to screen for Sclerotinia resistance in the greenhouse, growth chamber and field are also provided.

Abstract: The present invention relates to a commercially viable process for in vitro mass culture of Jatropha curcas. The process for in vitro mass culture of Jatropha curcas is simple, faster, and suitable for production of disease-free root tubers of uniform quality and employs media with a reduced concentration of phytohormones.

Abstract: A process of priming mature, imbibed, germinated somatic embryos of a conifer species. The process comprises fully immersing mature, imbibed, germinated somatic conifer embryos in a sterile liquid nutrient medium, bubbling an oxygen-containing gas through the liquid nutrient medium containing the germinants to form an aerated embryo-containing nutrient medium, optionally exposing the aerated germinant-containing nutrient medium to light, at least intermittently, continuing the immersion, bubbling and optional exposure to light for a period of time effective to produce at least some successfully primed conifer germinants that are adapted for subsequent planting and conversion to viable seedlings, and removing the successfully primed conifer germinants from the nutrient medium.

Abstract: The present invention relates to culturing isolated microspores and the subsequent generation of doubled-haploid plant lines that are suitable for the rapid selection of plants with improved composition and agronomic performance. Processes developed for the recovery of microspore-derived embryos from fennel and caraway may be adapted to related species in the Apiaceae family including, but not limited to, fennel, carrot, dill, anise, lovage, parsnip, and laceflower.

Abstract: The present invention provides a method of micropropagating a monocotyledonous plant comprising: (a) cultivating an explant of tissue from a monocotyledonous plant shoot tip on a primary medium, wherein the explant has been pretreated with a cold temperature and the primary medium comprises auxin or auxin and cytokinin, to produce a totipotent embryogenic cell culture; (b) treating the totipotent embryonic cell culture with a cold temperature; (c) maintaining the totipotent embryogenic cell culture by cultivation on a secondary medium, whereby a totipotent embryogenic cell culture of a monocotyledonous plant is produced and maintained; and (d) transferring the embryogenic cell culture of step (c) to a tertiary medium to continue multiplication and to produce a plantlet with roots and shoots, thereby micropropagating a monocotyledonous plant. The micropropagation techniques described herein provide plants for such purposes as development of elite plant lines, phytoremediation and biomass production.

Abstract: A determinate pea plant, where peas of the pea plant have a wrinkled-seed phenotype, and where the pea plant is slow-maturing.

Abstract: This disclosure describes an improved heat transfer system for use in reaction vessels used in chemical and biological processes. In one embodiment, a heat transfer baffle comprising two sub-assemblies adjoined to one another is provided.

Abstract: In various embodiments, methods described herein comprise the use of water-soluble cationic fullerene derivatives for improving plant genetic transformation. Cationic Fullerene derivatives of the invention possess DNA binding and compaction activity and provide a new method to deliver DNA into plant cells for plant transformation. Water-soluble fullerene derivatives of the invention with anionic or non-polar substituents possess antioxidant (free radical scavenging) activity, provide improved yields and efficiency of plant transformation methods such as biolistic, Agrobacterium tumefaciens, or electroporation methods by limiting cellular damage and resulting cell death leading to higher yields of viable transformed cells in the process.

Abstract: The invention provides a nutrient medium useful for sowing a somatic plant embryo or germinant of conifer species, which comprises particles of a solid component present within a flowable or semi-solid component containing water and a carbohydrate nutrient for the embryos or germinants. The nutrient medium has a fluidity such that at least some of the flowable or semi-solid component containing the carbohydrate nutrient remains in contact with the embryos or germinant at least until the embryo or germinant establishes vigorous growth under environmental conditions effective for such growth. The particles of the solid component are adapted to remain in contact with the embryo or germinant after of the flowable or semi-solid material dissipates, thereby providing continuing physical support for the embryo or germinant after the dissipation.

Abstract: A method of mass producing potato seedlings, involves collecting growing points of seed potatoes and culturing the growing points in a liquid or solid medium; introducing in vitro plantlets obtained from the culture of the growing points to solid culture; and removing the in vitro plantlets from the solid culture, and planting through stem cutting and acclimatizing the in vitro plantlets in deep flow culture, in which a nutrient solution is circulating. Upon planting in hydroponic facilities, such potato seedlings have high adaptability to the external environment and thus rapidly, uniformly generate roots in a short time. The rapid root anchoring prevents planted seedlings from withering, leading to death, growing poorly, and the like. The direct planting of in vitro plantlets through stem cutting without a separate acclimatization process shortens the overall production period of potato seedlings by omitting the acclimatization process.