Abstract: Methods to generate and isolate novel Synchronized in vitro cell strains of Muscadinia sp. “Noble”var. and North American grape germplasm containing flavonoid compounds.

Abstract: The present invention provides a method for the production of at least one fatty acid and/or oil from a plant cell suspension culture, the method comprising (i) maintaining a cell suspension culture of oil-producing plant cells under conditions such that the cultured cells synthesise and secrete at least one fatty acid and/or oil into the cell suspension culture medium; and (ii) extracting the thus secreted at least one fatty acid and/or oil from the cell suspension culture medium.

Abstract: The present invention provides a method for producing a true-to-type clone of an Aloe b?rb?densis mother plant by selecting an Aloe b?rb?densis mother plant; isolating a meristematic explant from the plant; culturing the meristematic explant in initiation medium to generate shoots, where the media lacks hormones; culturing the shoots in proliferation and elongation medium to generate elongated shoots, where the proliferation and elongation media comprises benzyl adenine (BA) and indole butyric acid (IBA); culturing the elongated shoots in rooting medium to generate plantlets, where the media lacks hormones; and culturing the plantlets to product a true-to-type clone of the Aloe b?rb?densis mother plant.

Abstract: This invention is a method of minimizing the variation of cell growth and production through homogeneous cell line development. To be more specific, it is the method of isolating and proliferating single cell clone from procambium to promote the stability of the plant-derived biologically active substances production by solving the problems of decrease in cell growth and the productivity during the long term culture.

Abstract: This invention is a method of minimizing the variation of cell growth and production through homogeneous cell line development. To be more specific, it is the method of isolating and proliferating single cell clone from cambium to promote the stability of the plant-derived biologically active substances production by solving the problems of decrease in cell growth and the productivity during the long term culture.

Abstract: Disclosed are bacteria and methods for promoting growth of Racomitrium canescens, tobacco, barley and soybean. The bacteria are selected from the methanol-utilizing bacteria deposited under Accession numbers FERM BP-11078, FERM BP-11079, FERM BP-11080 and FERM BP-11071, respectively which bacteria belong to the genus Methylobacterium. The methods are a method for promoting growth of protonemata of Racomitrium canescens which method is characterized by culturing the protonemata in the presence of the bacteria and a method for promoting growth of a seed plant selected from the group consisting of tobacco, barley and soybean which method is characterized by culturing seeds of the seed plant while contacting the seeds with the bacterium deposited under Accession number FERM BP-11078 which is one of the above-mentioned bacteria.

Abstract: The present invention provides a method of micropropagating a monocotyledonous plant comprising: (a) cultivating an explant of tissue from a monocotyledonous plant shoot tip on a primary medium, wherein the explant has been pretreated with a cold temperature and the primary medium comprises auxin or auxin and cytokinin, to produce a totipotent embryogenic cell culture; (b) treating the totipotent embryonic cell culture with a cold temperature; (c) maintaining the totipotent embryogenic cell culture by cultivation on a secondary medium, whereby a totipotent embryogenic cell culture of a monocotyledonous plant is produced and maintained; and (d) transferring the embryogenic cell culture of step (c) to a tertiary medium to continue multiplication and to produce a plantlet with roots and shoots, thereby micropropagating a monocotyledonous plant. The micropropagation techniques described herein provide plants for such purposes as development of elite plant lines, phytoremediation and biomass production.

Abstract: Provided are methods for introducing a sequence specific nuclease into a plant cell comprising a cell wall. Methods are provided for genetically or otherwise modifying plants and for treating or preventing disease in plant cells comprising a cell wall.

Abstract: This invention is intended to be used to search for a transcription factor having novel functions of increasing the weight of an individual plant, increasing the weight of a given tissue per individual plant, or improving the productivity of a given substance per individual plant and to improve such properties in the plant. The weight of an individual plant is increased, the weight of a given tissue per individual plant is increased, the productivity of a given substance per individual plant is improved, or the content of a given substance per given tissue of a plant is increased via expression of a transcription factor that has been modified to suppress transcription accelerating activity.

Abstract: The present invention describes a method for increasing the survival of the bacteria of Rhizobium genus, comprising the steps of: making the bacteria to grow in a chemically defined medium; keeping the bacteria in growth stationary phase for a proper period of time; exposing the bacteria to effective quantities of indole-3-acetic acid (IAA). Within the invention scope there is an alternative method to increase the survival of the bacteria of the Rhizobium genus by means of genetic engineering comprising the steps of: making a recombinant vector codifying enzymes able to produce IAA to express in effective way in said bacteria; making the bacteria to grow in chemically defined culture medium; keeping the bacteria in growth stationary phase for a proper period of time.

Abstract: The present invention relates to isolated polypeptides having cellulolytic enhancing activity and isolated polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides.

Abstract: The present invention relates to the field of somatic embryo production, particularly to methods for the regeneration of Jatropha through somatic embryogenesis. More specifically, the present invention relates to a method and media compositions for regeneration of plants of Jatropha curcas. The method is well suited for Jatropha curcas transformation and for producing clonal planting stock useful for large scale Jatropha curcas plantation.

Abstract: The present invention relates to boron transporters and, in particular, to boron transporters derived from plants. The present invention also relates to methods that utilise boron transporters, such as methods for modulating boron transport in cells and methods for determining the level or rate of boron transport in a cell or organism on the basis of the expression level of one or more boron transporters.

Abstract: The present invention provides an in vitro flavonoid-rich rhizome tissue of Neomarica gracilis, which is obtained from a tissue culture preparation of an N. gracilis tissue capable of proliferating, such as a root, a leaf, a basal portion of a leaf, and/or a rhizome. The in vitro flavonoid-rich rhizome tissue of N. gracilis contains tectorigenin, which is distinctively different from the naturally grown rhizome of N. gracilis which contains no tectorigenin. The present invention further provides a method for cultivating the in vitro flavonoid-rich rhizome tissue, a method for extracting the tectorigenin from the flavonoid-rich rhizome tissue, and quantitative methods for determining the amount of tectorigenin in the in vitro flavonoid-rich rhizome tissue.

Abstract: A hypobaric apparatus and methods capable of inducing and maintaining in stored horticultural cellular tissue controlled pervaporation of undesired chemical compounds without reaching the boiling point of intracellular water molecules, comprising a hypobaric chamber constructed to provide a leak rate of less than 4.0 mm Hg per hour, sensors and regulators coupled to the chamber and to a controller to measure and regulate target correlates. Commodities processed, including processing storage periods longer than any known involving controlled atmosphere, exhibit an absence of low oxygen injury, high carbon dioxide injury, chilling injury, leaf abscission, leaf de-greening, fungal decay, bacterial decay, gravitational curving, geotropic curvatures, leaf epinasty, stem epinasty, flower fading, senescence, live invertebrates at any life stage, fumigant chemical compounds, volatile fungicides, volatile bactericides; no advance in ripening; minimal water loss.

Abstract: The illustrated embodiment comprises a biosensor utilizing plant cells and nutrient media for maintaining the plant cells in a live condition. A light source having desired optical characteristics is directed onto the plant cells and light spectra transmitted from the cells is detected by a photodetector. A controller analyzes signals from the photodetector to detect a state change in the plant cells in response to exposure to an agent.

Abstract: Bioreactors suitable for housing a predetermined volume of culture medium comprising a plurality of containers of approximately equal internal volume in which the culture medium resides, wherein the predetermined volume of culture medium is retained in approximately equal amounts within each of the plurality of containers during operation of the bioreactor, and wherein the internal volume of each container is selected so that the amount of culture medium in each container exceeds 50 vol. % of the container internal volume during operation of the bioreactor, and related methods of use.

Abstract: The present invention relates to a cell line derived from the quiescent center of a plant and a method for isolating the same, more specifically, relates to a quiescent center-derived homogeneous cell line of single-cell origin, which is obtained from the quiescent center of a plant without needing a separate de-differentiation process, and a method for isolating the same.

Abstract: The present invention relates to a method of stabilizing a biological sample, having the steps of preparation of a biological sample, and of contacting the biological sample with a composition, having a substance according to the following structural formula: in which R1 is a hydrogen residue or a methyl residue, R2 and R3 are identical or different hydrocarbon residues with a length of the carbon chain of 1-20, and R4 is an oxygen, sulfur or selenium residue (FIG. 1).

Abstract: A handheld tool is disclosed which may be used to transfer a plurality of plant tissue explants from a first container to a second container. The handheld tool may include a disposable tip member which couples the plurality of plant tissue explants through use of negative pressure. An automated system which transfers a plurality of plant tissue explants from a first container to a second container is also disclosed. The automated system may include a first presentment system which moves the first container to a region, a second presentment system which moves the second container to the region, and a robot system that transfers the plurality of plant tissue explants from the first container to the second container.

Abstract: The present invention relates to a cell line derived from the cambium of an herbaceous plant having a storage root and a method for isolating the same. More specifically, relates to a cambium-derived homogeneous cell line having the ability to divide, which is obtained from the cambium-containing storage tissue of an herbaceous plant having a storage root without a separate dedifferentiation process, and to a method for isolating the same. The cell line derived from the cambium of an herbaceous plant having a storage root has active division ability and is homogeneous. Also, it is stable during culture, because it has not undergone a dedifferentiation process. Thus, through the optimization of proliferation thereof, the cell line can be allowed to proliferate in a large amount within a short time.

Abstract: A method of sowing a somatic plant embryo or germinant of a conifer species to facilitate subsequent development of the embryo or germinant into an autotrophic seedling. The method involves the following steps carried out ex vitro in non-sterile conditions: providing a nutrient medium comprising particles of a solid component present within a flowable or semi-solid component containing water and a carbohydrate nutrient for the embryo or germinant, dispensing a quantity of the nutrient medium onto a surface of a porous solid growth substrate for the somatic plant embryo or germinant, and contacting the plant embryo or germinant with the nutrient medium. The nutrient medium has a fluidity such that at least some of the flowable or semi-solid component containing the carbohydrate nutrient remains in contact with the embryo or germinant at least until the embryo or germinant establishes vigorous growth under environmental conditions effective for such growth.

Abstract: The present invention relates to cell, tissue or derivatives thereof, preserving compositions for cells in culture, storage, or lyophilization. According to the invention ergothioneine is added as a supplement to such extender or preservative compositions. Sperm cells so treated showed increased motility when thawed compared to cells that were not treated with ergothioneine.

Abstract: The present invention is directed to methods and compositions of endophytic fungi that confer stress tolerance to inoculated plants, including both monocots and dicots. In particular, Fusarium species, isolated from the dunegrass, Leymus mollis, growing in plant communities on Puget Sound beaches of Washington State. Upon inoculating a target plant or plant part with the endophytic fungi, the resulting plant shows stress tolerance, particularly drought and salinity tolerance.

Abstract: One of the problems to be resolved by the present invention is to stably and sustainably produce active ingredients contained in Bignoniaceous plants of which a mass production is difficult in a conventional manner. The present invention relates to a method for efficiently preparing an anticancer active ingredient NQ801 by a cell cultivation of Bignoniaceous plants under specific culture conditions. An ingredient-production system in the present invention has an anticancer activity.

Abstract: A description is given of a bioreactor (1), in particular for NMR spectroscopy, comprising a container (7) capable of containing a cell culture, a first inlet line (6) for the inward flow of a culture medium to the inside of the container and a second outlet line (9, 10) for the outward flow of the culture medium from the container (7). The first line (6), an inlet line, is connected to a spiral-shaped device (12) which has a form such that when the medium is made to flow inside the first line (6) and made to flow out of the second line (9; 10), hydrostatic thrust and hydrodynamic forces produce with respect to the cells a condition of simulated reduced gravity inside the container (7).

Abstract: The invention provides seed and plants of the bean line designated RS08051531. The invention thus relates to the plants, seeds and tissue cultures of bean line RS08051531, and to methods for producing a bean plant produced by crossing a plant of bean line RS08051531 with itself or with another bean plant, such as a plant of another line. The invention further relates to seeds and plants produced by such crossing. The invention further relates to parts of a plant of bean line RS08051531, including the pods and gametes of such plants.

Abstract: A novel garden bean cultivar, designated SB4285, is disclosed. The invention relates to the seeds of garden bean cultivar SB4285, to the plants of garden bean line SB4285 and to methods for producing a garden bean plant by crossing the cultivar SB4285 with itself or another garden bean line. The invention further relates to methods for producing a garden bean plant containing in its genetic material one or more transgenes and to the transgenic plants produced by that method and to methods for producing other garden bean lines derived from the cultivar SB4285.

Abstract: A tissue culture device includes a container having one or more upstanding walls extending upwardly from a floor. The floor of the container has a media having nutrients or growth substances therein. A plurality of plant tissues are within the container compartment and are placed upon a screen between the plant tissues and the media. The screen is tamped downwardly onto the media so that the plant tissues can get nutrients from the media, and so that waste products are transferred into the media. The screen is also removable through the open end of the container so as to remove all of the plurality of the plant tissues from the container at once. The plant tissue device can then be placed in another container having any cultured media as needed for the correct maintenance, propagation and development of plant tissue in culture.

Abstract: The present invention relates to a method of producing corosolic acid by using plant cells that produce corosolic acid. More particularly, the present invention relates to a method of producing corosolic acid by using plant cell suspension cultures comprising the steps of: inducing a callus from a tissue of a plant producing corosolic acid; preparing a cell line capable of being cultured in liquid culture medium from the induced callus; culturing the cell line in a suspension culture; and isolating corosolic acid from the culture solution. The present invention has advantage of maximizing productivity by utilizing two-stage culture, treatment with inducing agent, and high cell-density culture in the suspension culture of plant cells producing corosolic acid.

Abstract: The invention provides methods for producing pine somatic embryos using a liquid development medium and/or a liquid stratification medium. In a first aspect, the methods comprise the step of culturing embryogenic cells in, or on, a liquid development medium to produce cotyledonary pine somatic embryos. In another aspect, the methods comprise the step of culturing pine cotyledonary somatic embryos in, or on, a liquid stratification medium to produce stratified cotyledonary somatic embryos. The invention also provides methods for producing pine somatic embryos in bioreactors.

Abstract: A method for storing a water-soluble undissolved silica-based material, optionally with an incorporated functional agent. The method includes immersing the material in an aqueous liquid phase which is initially saturated with a water-soluble silica-based material which is the same or different than the undissolved silica-based material, such that the undissolved silica-based material does not essentially dissolve into the liquid phase during storage, or the proportion of the amount of the initially undissolved silica-based material to the amount of the liquid phase is such that the liquid phase will be saturated or essentially saturated by a dissolved portion of the water-soluble undissolved silica-based material during storage, which dissolved portion of said water soluble undissolved silica-based material is less than 20%. Also disclosed is a storage package which may preferably be used to store functional agents, delivery devices or implants.

Abstract: A method of formulating high ambient temperature (room temperature and above) stable biologics (biologically active macromolecules, enzymes, serums, vaccines, viruses, pesticides, drug delivery systems, liposomes, cells suspensions, sperm, erythrocytes, other blood cells, stem cells, multicellular tissues, skin, heart valves) including secondary drying comprising at least two steps of stability drying at elevated temperature: 35° C., 40° C., 45° C., 50° C., and higher temperatures. The method could be applied to stabilize biologics encapsulated in alginate gel microspheres for better oral delivery. The method encompasses the following: microspheres are formulated using a cryo-encapsulation procedure comprised of mixing drops of frozen preservation mixture (To form the preservation mixture, biologics are mixed with preservation solutions containing sodium alginate.) with frozen drops of a calcium solution (i.e. calcium gluconate) and subsequent warming to form the gel particles.

Abstract: The invention provides seed and plants of the pea line designated 08550821. The invention thus relates to the plants, seeds and tissue cultures of pea line 08550821, and to methods for producing a pea plant produced by crossing a plant of pea line 08550821 with itself or with another pea plant, such as a plant of another line. The invention further relates to seeds and plants produced by such crossing. The invention further relates to parts of a plant of pea line 08550821, including the seed, pod, and gametes of such plants.

Abstract: The invention provides seed and plants of the pea line designated 08510617. The invention thus relates to the plants, seeds and tissue cultures of pea line 08510617, and to methods for producing a pea plant produced by crossing a plant of pea line 08510617 with itself or with another pea plant, such as a plant of another line. The invention further relates to seeds and plants produced by such crossing. The invention further relates to parts of a plant of pea line 08510617, including the seed, pod, and gametes of such plants.

Abstract: The invention provides seed and plants of the pea line designated Crescendo. The invention thus relates to the plants, seeds and tissue cultures of pea line Crescendo, and to methods for producing a pea plant produced by crossing a plant of pea line Crescendo with itself or with another pea plant, such as a plant of another line. The invention further relates to seeds and plants produced by such crossing. The invention further relates to parts of a plant of pea line Crescendo, including the seed, pod, and gametes of such plants.

Abstract: The invention provides seed and plants of the pea line designated 08240773. The invention thus relates to the plants, seeds and tissue cultures of pea line 08240773, and to methods for producing a pea plant produced by crossing a plant of pea line 08240773 with itself or with another pea plant, such as a plant of another line. The invention further relates to seeds and plants produced by such crossing. The invention further relates to parts of a plant of pea line 08240773, including the seed, pod, and gametes of such plants.

Abstract: The invention provides seed and plants of the bean line designated RS08051272. The invention thus relates to the plants, seeds and tissue cultures of bean line RS08051272, and to methods for producing a bean plant produced by crossing a plant of bean line RS08051272 with itself or with another bean plant, such as a plant of another line. The invention further relates to seeds and plants produced by such crossing. The invention further relates to parts of a plant of bean line RS08051272, including the pods and gametes of such plants.

Abstract: The invention provides seed and plants of the bean line designated EX08061812. The invention thus relates to the plants, seeds and tissue cultures of bean line EX08061812, and to methods for producing a bean plant produced by crossing a plant of bean line EX08061812 with itself or with another bean plant, such as a plant of another line. The invention further relates to seeds and plants produced by such crossing. The invention further relates to parts of a plant of bean line EX08061812, including the pods and gametes of such plants.

Abstract: The invention provides seed and plants of the pea line designated 08520702. The invention thus relates to the plants, seeds and tissue cultures of pea line 08520702, and to methods for producing a pea plant produced by crossing a plant of pea line 08520702 with itself or with another pea plant, such as a plant of another line. The invention further relates to seeds and plants produced by such crossing. The invention further relates to parts of a plant of pea line 08520702, including the seed, pod, and gametes of such plants.

Abstract: The invention provides seed and plants of the pea line designated 08540794. The invention thus relates to the plants, seeds and tissue cultures of pea line 08540794, and to methods for producing a pea plant produced by crossing a plant of pea line 08540794 with itself or with another pea plant, such as a plant of another line. The invention further relates to seeds and plants produced by such crossing. The invention further relates to parts of a plant of pea line 08540794, including the seed, pod, and gametes of such plants.

Abstract: The invention provides seed and plants of the pea line designated 08560899. The invention thus relates to the plants, seeds and tissue cultures of pea line 08560899, and to methods for producing a pea plant produced by crossing a plant of pea line 08560899 with itself or with another pea plant, such as a plant of another line. The invention further relates to seeds and plants produced by such crossing. The invention further relates to parts of a plant of pea line 08560899, including the seed, pod, and gametes of such plants.

Abstract: The invention provides seed and plants of the bean line designated 08560863. The invention thus relates to the plants, seeds and tissue cultures of bean line 08560863, and to methods for producing a bean plant produced by crossing a plant of bean line 08560863 with itself or with another bean plant, such as a plant of another line. The invention further relates to seeds and plants produced by such crossing. The invention further relates to parts of a plant of bean line 08560863, including the pods and gametes of such plants.

Abstract: This invention provides novel methods for improving plant quality and yield in the presence of pathogens. The method increases the levels of pathogenesis-related proteins, such as PR1, phenylalanine ammonia lyase, or plant cell wall proteins such as hydroxyproline-rich glycoproteins, in a plant by contacting the plant with a plant systemic inducer and a reactive oxygen species wherein the amount of the reactive oxygen species is sufficient to increase the amount of the pathogenesis-related protein above the level induced by the plant systemic inducer in the absence of the reactive oxygen species. A preferred reactive oxygen species is peracetic acid; a preferred plant systemic inducer is salicylic acid.

Abstract: In one aspect, the present invention provides methods of storing conifer somatic embryo germinants germinated on a sterile germination medium for delayed transplanting into a growth medium. The methods of the invention comprise the steps of: (a) placing the germinants while still on sterile germination medium into a cold environment in which the temperature is in the range of 0.5° C. to 10° C. for a time period up to six months, said germinants comprising a visible, well-defined epicotyl and radicle; and (b) placing the germinants in water for a time greater than about 1 hour at a temperature below about 24° C. prior to transplantation into growth medium.

Abstract: The present invention relates to a novel transformation system for generating transformed corn plants. In particular, the invention relates to a rapid selection system at an elevated temperature that allows faster and more efficient transformation.

Abstract: The present invention aims to provide a method for increasing transformation efficiency in plants when compared to conventionally known Agrobacterium-mediated methods. In the present invention, one of the features is to comprise a coculture step for culturing an Agrobacterium-inoculated plant tissue with a coculture medium containing 3,6-dichloro-o-anisic acid.

Abstract: The promoter of a soybean metallothionein protein (MTH1) and fragments thereof and their use in promoting the expression of one or more heterologous nucleic acid fragments in a tissue-independent or constitutive manner in plants are described.

Abstract: The invention provides seed and plants of the bean line designated EX 15340804. The invention thus relates to the plants, seeds and tissue cultures of bean line EX 15340804, and to methods for producing a bean plant produced by crossing a plant of bean line EX 15340804 with itself or with another bean plant, such as a plant of another line. The invention further relates to seeds and plants produced by such crossing. The invention further relates to parts of a plant of bean line EX 15340804, including the pods and gametes of such plants.

Abstract: The invention provides seed and plants of the pea line designated 08520689. The invention thus relates to the plants, seeds and tissue cultures of pea line 08520689, and to methods for producing a pea plant produced by crossing a plant of pea line 08520689 with itself or with another pea plant, such as a plant of another line. The invention further relates to seeds and plants produced by such crossing. The invention further relates to parts of a plant of pea line 08520689, including the seed, pod, and gametes of such plants.