Abstract: Polymeric thin-film tube connectors, bioreactors, systems and methods are described, the connectors allowing connection of a polymeric thin-film tube of a bioreactor chamber with a complementary part, typically, rigid port of flange of the bioreactor or bioreactor system. Further, bioreactors having a thin-film reactor chamber and one or more connectors are described. The thin-film reactor chamber can have a thin-film wall for enclosing culture medium and microorganisms. The connector can include a flexible boot. An opening of the thin-film wall couples to the flexible boot. The thin-film wall can extend through a reactor end of the flexible boot and can rest against an interior surface of the flexible boot. The connectors are particularly advantageous for connecting standard utilities in a bioreactor system to thin-film bioreactor chambers or capsules having a thin-film tubular opening.

Abstract: The present invention relates to the production of alkaloids from Liliaceae cell culture.

Abstract: The present invention provides novel lettuce cultivar Stage Coach and plant parts, seed, and tissue culture therefrom. The invention also provides methods for producing a lettuce plant by crossing the lettuce plants of the invention with themselves or another lettuce plant. The invention also provides lettuce plants produced from such a crossing as well as plant parts, seed, and tissue culture therefrom.

Abstract: In one aspect, a method is provided for producing an improved nutritive medium comprising an adsorbent material for culturing plant embryos.

Abstract: The present invention provides methods for transforming monocot plants via a simple and rapid protocol, to obtain regenerated plants capable of being planted to soil in as little as 4-8 weeks. Associated cell culture media and growth conditions are also provided, as well as plants and plant parts obtained by the method. Further, a method for screening recalcitrant plant genotypes for transformability by the methods of the present invention is also provided. Further, a system for expanding priority development window for producing transgenic plants by the methods of the present invention is also provided.

Abstract: A method of policy management and control on a mobile phone. The method comprises receiving a user interface change request by a launcher application executing on the mobile phone, requesting permissions from an interface control application programming interface (API) by the launcher application, receiving a response from the interface control application programming interface by the launcher application, where the response indicates if the user interface change is permitted, and enforcing the received permission by the launcher application, where enforcing the received permission comprises executing the user interface change request when the user interface change is permitted, and where enforcing the received permissions comprises not executing the user interface change request and presenting a message to a user of the mobile phone when the user interface change is restricted.

Abstract: The present invention relates to a method for fabricating a patterned substrate for a cell culture, comprising the steps of: (1) preparing a substrate; (2) depositing a plasma polymer layer by using a precursor material on the substrate; (3) placing a shadow mask having a predetermined pattern on the plasma polymer layer; (4) treating the substrate, having the shadow mask placed thereon, with a reactive gas using plasma; and (5) removing the shadow mask from the substrate, and a patterned substrate for the cell culture fabricated thereby. The invention also relates to a method for a cell culture with a pattern, comprising the step of culturing cells on the patterned substrate for the cell culture, and a patterned cell chip, and a method of screening a material having an activity of inducing or promoting angiogenesis using the patterned cell chip.

Abstract: The present invention provides novel snap pea cultivar SL3134 and plant parts, seed, and tissue culture therefrom. The invention also provides methods for producing a pea plant by crossing the pea plants of the invention with themselves or another pea plant. The invention also provides pea plants produced from such a crossing as well as plant parts, seed, and tissue culture therefrom.

Abstract: Disclosed herein are media, kits, systems and methods for achieving micropropagation of bamboo on a commercially-relevant scale.

Abstract: The present invention provides methods and devices for the rapid isolation of monocot plant embryos suitable for transformation or tissue culture. The invention includes mechanical devices for substantially isolating plant embryos for use as transformable explants. Media suitable for isolating plant embryos and methods for their preparation are also provided.

Abstract: A method to generate siRNAs in vivo is described, as are constructs and compositions useful in the method. The method does not depend on the use of DNA or synthetic constructs that contain inverted duplications or dual promoters so as to form perfect or largely double-stranded RNA. Rather, the method depends on constructs that yield single-stranded RNA transcripts, and exploits endogenous or in vivo-produced miRNAs or siRNAs to initiate production of siRNAs. The miRNAs or siRNAs guide cleavage of the transcript and set the register for production of siRNAs (usually 21 nucleotides in length) encoded adjacent to the initiation cleavage site within the construct. The method results in specific formation of siRNAs of predictable size and register (phase) relative to the initiation cleavage site. The method can be used to produce specific siRNAs in vivo for inactivation or suppression of one or more target genes or other entities, such as pathogens.

Abstract: The present invention provides transgenic plants transformed with exogenous nucleic acid encoding a Dunaliella plasma membrane (PM)-ATPase. The transgenic plant has increased tolerance to salt as compared to a corresponding non-transgenic plant. The present invention also provides nucleic acids encoding a chimeric PM-ATPase, which comprise a first portion encoding a plant PM-ATPase or a fragment thereof, and a second portion encoding a Dunaliella PM-ATPase or a fragment thereof. The present invention also discloses a method of producing a transgenic plant having an increased tolerance to salt as compared to a corresponding non-transgenic plant, a method of modifying a plant capacity to survive salt shock, and a method of modifying plant recovery after exposure to salt stress, by introducing into one or more cells of a plant an exogenous nucleic acid encoding a Dunaliella PM-ATPase.

Abstract: The invention comprises of an implementation of the lyophilization process in a method for a large-scale preservation of the entire biological material prior to the extraction and/or the utilization/manipulation of any substances of the biological content. The drying-conservation of all the natural substances is achieved via deep-freezing evaporation followed by complete drying of the biological material by the means of sublimation, and other additional manipulations and procedures suitable for the preparation of various commercially viable products further on.

Abstract: A soybean cultivar designated S120116 is disclosed. The invention relates to the seeds of soybean cultivar S120116, to the plants of soybean cultivar S120116, to the plant parts of soybean cultivar S120116, and to methods for producing progeny of soybean cultivar S120116. The invention also relates to methods for producing a soybean plant containing in its genetic material one or more transgenes and to the transgenic soybean plants and plant parts produced by those methods. The invention also relates to soybean cultivars or breeding cultivars, and plant parts derived from soybean cultivar S120116. The invention also relates to methods for producing other soybean cultivars, lines, or plant parts derived from soybean cultivar S120116, and to the soybean plants, varieties, and their parts derived from use of those methods. The invention further relates to hybrid soybean seeds, plants, and plant parts produced by crossing cultivar S120116 with another soybean cultivar.

Abstract: A photobioreactor apparatus in which a reaction chamber contains a slurry of water, nutrients and photosynthetic organisms. The sidewall of the reaction chamber is constructed of translucent material, thereby permitting light to be conveyed into the slurry to promote photosynthesis. The interior of the reaction chamber is in fluid communication with a gas dispenser. A lift member is disposed in the reaction chamber and gas is introduced in the slurry beneath the lift member, thereby providing buoyancy to lift the lift member, thereby providing agitation and movement of the slurry within the photobioreactor and enhancing growth of photosynthetic organisms.

Abstract: A heat exchanger system includes a heat exchanger having a body plate with a first side face and an opposing second side face, the body plate bounding a fluid channel disposed between the opposing side faces and extending between an inlet port and an outlet port, and a first side plate having an inside face. A flexible first bag includes of one or more sheets of polymeric material, the first bag bounding a fluid pathway that extends between a fluid inlet and a fluid outlet, the first bag being removably retained between the body plate and the first side plate so that at least a portion of the first bag rests against the first side face of the body plate when the fluid pathway of the first bag is filled with a fluid.

Abstract: A soybean cultivar designated 25261523 is disclosed. The invention relates to the seeds of soybean cultivar 25261523, to the plants of soybean cultivar 25261523, to the plant parts of soybean cultivar 25261523, and to methods for producing progeny of soybean cultivar 25261523. The invention also relates to methods for producing a soybean plant containing in its genetic material one or more transgenes and to the transgenic soybean plants and plant parts produced by those methods. The invention also relates to soybean cultivars or breeding cultivars, and plant parts derived from soybean cultivar 25261523. The invention also relates to methods for producing other soybean cultivars, lines, or plant parts derived from soybean cultivar 25261523, and to the soybean plants, varieties, and their parts derived from use of those methods. The invention further relates to hybrid soybean seeds, plants, and plant parts produced by crossing cultivar 25261523 with another soybean cultivar.

Abstract: Provide are a peptide gel with practically sufficient mechanical strength and a self-assembling peptide capable of forming the peptide gel. The self-assembling peptide is formed of the following amino acid sequence: a1b1c1b2a2b3db4a3b5c2b6a4 where: a1 to a4 each represent a basic amino acid residue; b1 to b6 each represent an uncharged polar amino acid residue and/or a hydrophobic amino acid residue, provided that at least five thereof each represent a hydrophobic amino acid residue; c1 and c2 each represent an acidic amino acid residue; and d represents a hydrophobic amino acid residue.

Abstract: This invention relates to isolated nucleic acid fragments encoding polypeptides involved in post-transcriptional gene silencing. The invention also relates to construction of a recombinant DNA construct encoding all or a portion of the polypeptide involved in post-transcriptional gene silencing, in sense or antisense orientation, wherein expression of the recombinant DNA construct results in production of altered levels in a transformed host cell of the polypeptide involved in post-transcriptional gene silencing.

Abstract: According to the invention, there is provided seed and plants of the corn variety designated CV636214. The invention thus relates to the plants, seeds and tissue cultures of the variety CV636214, and to methods for producing a corn plant produced by crossing a corn plant of variety CV636214 with itself or with another corn plant, such as a plant of another variety. The invention further relates to corn seeds and plants produced by crossing plants of variety CV636214 with plants of another variety, such as another inbred line. The invention further relates to the inbred and hybrid genetic complements of plants of variety CV636214.

Abstract: A soybean cultivar designated 17110882 is disclosed. The invention relates to the seeds of soybean cultivar 17110882, to the plants of soybean cultivar 17110882, to the plant parts of soybean cultivar 17110882, and to methods for producing progeny of soybean cultivar 17110882. The invention also relates to methods for producing a soybean plant containing in its genetic material one or more transgenes and to the transgenic soybean plants and plant parts produced by those methods. The invention also relates to soybean cultivars or breeding cultivars, and plant parts derived from soybean cultivar 17110882. The invention also relates to methods for producing other soybean cultivars, lines, or plant parts derived from soybean cultivar 17110882, and to the soybean plants, varieties, and their parts derived from use of those methods. The invention further relates to hybrid soybean seeds, plants, and plant parts produced by crossing cultivar 17110882 with another soybean cultivar.

Abstract: A novel garden bean cultivar, designated SB4534, is disclosed. The invention relates to the seeds of garden bean cultivar SB4534, to the plants of garden bean line SB4534 and to methods for producing a garden bean plant by crossing the cultivar SB4534 with itself or another garden bean line. The invention further relates to methods for producing a garden bean plant containing in its genetic material one or more transgenes and to the transgenic plants produced by that method and to methods for producing other garden bean lines derived from the cultivar SB4534.

Abstract: The present invention relates to the preparation of plant cell cultures belonging to the Dendrobium Phalaenopsis, Ansellia, Polyrrhiza, Vanilla, Cattleya and Vanda genera and their use in cosmetic, nutritional and pharmaceutical fields. In particular, the present invention regards a selected cell culture of meristematic cells of plants belonging to the Dendrobium Phalaenopsis, Ansellia, Polyrrhiza, Vanilla, Cattleya and Vanda genera characterized in that it contains an amount of phenylpropanoids greater than 0.1% expressed on the cell dry weight and an amount of hydrosoluble polysaccharides greater than 5% expressed on the cell dry weight, as well as preparations, pharmaceutical and cosmetic compositions or food supplements containing the cell culture.

Abstract: There is provided a process for effecting growth of phototrophic biomass within the reaction zone of a photobioreactor, comprising, after effecting at least a reduction in the molar rate of supply of carbon dioxide to the reaction zone of the photobioreactor, supplying a gaseous photobioreactor exhaust, that includes diatomic (or molecular) oxygen being generated by photosynthesis effected within the reaction zone by the supplied carbon dioxide, to a combustion zone of a combustor.

Abstract: The present invention relates to the field of somatic embryo production, particularly to methods for the regeneration of Jatropha through somatic embryogenesis. More specifically, the present invention relates to a method and media compositions for regeneration of plants of Jatropha curcas. The method is well suited for Jatropha curcas transformation and for producing clonal planting stock useful for large scale Jatropha curcas plantation.

Abstract: A method of classifying plant embryos according to their quality based on a general form of Lorenz-Bayes classifier is disclosed. First, image or spectral data of plant embryos of known quality are acquired, and the data are divided into two classes according to the embryos' known quality. Second, metrics are calculated from the acquired image or spectral data in each class. Third, multi-dimensional histograms of multiple metrics are prepared for both classes. Fourth, the difference or some other measure of comparison between the two multi-dimensional histograms is obtained. Fifth, image or spectral data of a plant embryo of unknown quality are obtained and metrics are calculated therefrom. Sixth, the embryo of unknown quality is assigned to a class based on its calculated metrics and the result of the comparison as calculated in the fourth step above.

Abstract: The invention relates to a reactor driven in an oscillatory-rotary manner about its fixed, preferably vertical, axis, for preferably biotechnological and pharmaceutical applications. By means of its process-intensifying properties for mixing, suspension, gaseous material transport, heat transfer, irradiation and particle retention, the applicability on the industrial scale is ensured. The reactor which succeeds without a shaft seal permits particularly robust production with respect to sterile technique with avoidance of cleaning and cleaning validation required when the reactor is constructed as a single-use reactor.

Abstract: Disclosed are methods for obtaining a natural product preparation with reduced glucosinolate contamination from a plant of Brassicaceae. The methods can include cultivating a plant callus from a plant capable of producing a desired natural product, selecting a callus with reduced glucosinolate production, and cultivating the selected callus in a liquid medium. The method can also include recovering the natural product from the culture. Also disclosed are methods for obtaining cabbage anthocyanin with reduced glucosinolate contamination. The methods can include cultivating a red cabbage plant callus with reduced glucosinolate production in a liquid medium to obtain a suspension culture and cultivating the suspension culture in a medium lacking a nitrogen source. The method can also include recovering the anthocyanin with reduced glucosinolate contamination from the culture. Finally, several specific low-glucosinolate cell lines are described.

Abstract: The invention provides seed and plants of the pea line designated EX 08260893. The invention thus relates to the plants, seeds and tissue cultures of pea line EX 08260893, and to methods for producing a pea plant produced by crossing a plant of pea line EX 08260893 with itself or with another pea plant, such as a plant of another line. The invention further relates to seeds and plants produced by such crossing. The invention further relates to parts of a plant of pea line EX 08260893, including the seed, pod, and gametes of such plants.

Abstract: The invention discloses a multi-layer cell freezing vial and method of ohtention thereof. The method of obtention is a cell freezing and thawing process that avoids the need of incorporating new fresh medium to the thawed cells for the dilution of the cryoprotective agent. This is achieved by the previous freezing of an extra layer of culture medium in addition to the frozen ceil solution containing said cryoprotective agent. At the time of thawing, the culture medium and the cell solution mix themselves, turning the concentration of said cryoprotective agent to a non-toxic dilution and achieving the effective exclusion of said cryoprotective agent from the living cells.

Abstract: The invention provides seed and plants of the bean line designated RX 08075309. The invention thus relates to the plants, seeds and tissue cultures of bean line RX 08075309, and to methods for producing a bean plant produced by crossing a plant of bean line RX 08075309 with itself or with another bean plant, such as a plant of another line. The invention further relates to seeds and plants produced by such crossing. The invention further relates to parts of a plant of bean line RX 08075309, including the pods and gametes of such plants.

Abstract: The invention provides seed and plants of the bean line designated FIVC6V1002. The invention thus relates to the plants, seeds and tissue cultures of bean line FIVC6V1002, and to methods for producing a bean plant produced by crossing a plant of bean line FIVC6V1002 with itself or with another bean plant, such as a plant of another line. The invention further relates to seeds and plants produced by such crossing. The invention further relates to parts of a plant of bean line FIVC6V1002, including the pods and gametes of such plants.

Abstract: The invention provides seed and plants of the bean line designated FMGC6V0958. The invention thus relates to the plants, seeds and tissue cultures of bean line FMGC6V0958, and to methods for producing a bean plant produced by crossing a plant of bean line FMGC6V0958 with itself or with another bean plant, such as a plant of another line. The invention further relates to seeds and plants produced by such crossing. The invention further relates to parts of a plant of bean line FMGC6V0958, including the pods and gametes of such plants.

Abstract: The invention provides seed and plants of the bean line designated FMGC6V1006. The invention thus relates to the plants, seeds and tissue cultures of bean line FMGC6V1006, and to methods for producing a bean plant produced by crossing a plant of bean line FMGC6V1006 with itself or with another bean plant, such as a plant of another line. The invention further relates to seeds and plants produced by such crossing. The invention further relates to parts of a plant of bean line FMGC6V1006, including the pods and gametes of such plants.

Abstract: Cell cultures of Vaccinium myrtillus configured to grow in suspension culture in a liquid medium. The cells are derived from one or more V. myrtillus plant parts, such as an edible plant part (e.g., a leaf part or a berry part) or a stem part. The cells are adapted to grow to a high density is a relatively short period of time (e.g., about 7 days). In addition, the cells are adapted to produce high concentrations of polyphenols and/or procyanidins and essentially no anthocyanin. Methods for production of polyphenols and/or procyanidins from Vaccinium myrtillus cells grown in suspension culture are disclosed.

Abstract: An apparatus for preparing plant tissue (e.g., somatic embryos, embryogenic tissue, organogenic tissue, vegetative tissue, seeds, etc.) for plant production includes a first station having a first rack system configured to support at least one culture vessel, a second station having an automated member configured to manipulate the at least one culture vessel and a third station having a second rack system configured to support the at least one culture vessel after being manipulated by the automated member. The second station can be selectively adjusted to perform more than one operation required in the plant development. According to exemplary embodiments, the apparatus may include more than one second station (e.g., operational stations, etc.).

Abstract: The invention provides seed and plants of the bean line designated FIVC6V0999. The invention thus relates to the plants, seeds and tissue cultures of bean line FIVC6V0999, and to methods for producing a bean plant produced by crossing a plant of bean line FIVC6V0999 with itself or with another bean plant, such as a plant of another line. The invention further relates to seeds and plants produced by such crossing. The invention further relates to parts of a plant of bean line FIVC6V0999, including the pods and gametes of such plants.

Abstract: The invention provides seed and plants of squash hybrid PX 13056719 and the parent lines thereof. The invention thus relates to the plants, seeds and tissue cultures of squash hybrid PX 13056719 and the parent lines thereof, and to methods for producing a squash plant produced by crossing such plants with themselves or with another squash plant, such as a plant of another genotype. The invention further relates to seeds and plants produced by such crossing. The invention further relates to parts of such plants, including the fruit and gametes of such plants.

Abstract: The invention provides seed and plants of squash hybrid RX 04858033 and the parent lines thereof. The invention thus relates to the plants, seeds and tissue cultures of squash hybrid RX 04858033 and the parent lines thereof, and to methods for producing a squash plant produced by crossing such plants with themselves or with another squash plant, such as a plant of another genotype. The invention further relates to seeds and plants produced by such crossing. The invention further relates to parts of such plants, including the fruit and gametes of such plants.

Abstract: Scalable up and down nesting integrated electronic enclosures with form factors including asteroids and/or dumbbells and/or approximated tessellation(s)/tiling(s) or combinations thereof with thermal management, wiring, sliding fit, manual and/or automated full range vertical to horizontal positioning, access and structural systems for individual modules and intra- and inter-planar stacks, columns, rows, arrays and associated infrastructures.

Abstract: The present invention provides methods and compositions for improving plant health. In particular, application of dicamba or another substrate of DMO, or metabolites thereof including DCSA, to a plant confers tolerance to, or defense against, abiotic or biotic stresses such as oxidative stress including herbicide application, and plant disease, and enhances crop yield. Such application may be in combination with the application of another herbicide such as glyphosate.

Abstract: A method of producing a stably transformed corn plant in a single container is demonstrated. This method allows for the automation of the transformation process and reduces labor, material, and ergonomic costs associated with traditional plant tissue culture systems.

Abstract: The invention provides seed and plants of the bean line designated FIVC6V0998. The invention thus relates to the plants, seeds and tissue cultures of bean line FIVC6V0998, and to methods for producing a bean plant produced by crossing a plant of bean line FIVC6V0998 with itself or with another bean plant, such as a plant of another line. The invention further relates to seeds and plants produced by such crossing. The invention further relates to parts of a plant of bean line FIVC6V0998, including the pods and gametes of such plants.

Abstract: The invention relates to a rucola plant of the species Diplotaxis tenuifolia which may comprise a genetic determinant that leads to expression of resistance against Peronospora parasitica, which genetic determinant is obtainable from a Diplotaxis tenuifolia plant which may comprise said genetic determinant, representative seed of which was deposited with the NCIMB under deposit number NCIMB 41811. The invention further provides seeds that may grow into a plant that is resistant to Peronospora parasitica, to progeny of the rucola plant to propagation material suitable for producing the plant or seed and to parts of the plant and food products which may comprise the plant parts.

Abstract: The invention provides seed and plants of the pea line designated EX08240782. The invention thus relates to the plants, seeds and tissue cultures of pea line EX08240782, and to methods for producing a pea plant produced by crossing a plant of pea line EX08240782 with itself or with another pea plant, such as a plant of another line. The invention further relates to seeds and plants produced by such crossing. The invention further relates to parts of a plant of pea line EX08240782, including the seed, pod, and gametes of such plants.

Abstract: Biocontainers, as well as methods and platforms for rocking the biocontainers, are disclosed.

Abstract: The present invention relates to a cell line derived from the cambium of an herbaceous plant having a storage root and a method for isolating the same. More specifically, relates to a cambium-derived homogeneous cell line having the ability to divide, which is obtained from the cambium-containing storage tissue of an herbaceous plant having a storage root without a separate dedifferentiation process, and to a method for isolating the same. The cell line derived from the cambium of an herbaceous plant having a storage root has an active division ability and is homogeneous. Also, it is stable during culture, because it has not undergone a dedifferentiation process. Thus, through the optimization of proliferation thereof, the cell line can be allowed to proliferate in a large amount within a short time.

Abstract: Certain embodiments disclosed herein include, but are not limited to, at least one of compositions, methods, devices, systems, kits, or products regarding rejuvenation or preservation of germ cells or gametes. Certain embodiments disclosed herein include, but are not limited to, methods of modifying germ cells or gametes, or methods of administering modified germ cells or gametes to at least one biological tissue.

Abstract: Disclosed herein are media, kits, systems and methods for achieving micropropagation of bamboo on a commercially-relevant scale.

Abstract: A fluid mixing system includes a support housing having an interior surface bounding a chamber. A flexible bag is disposed within the chamber of the support housing, the flexible bag having an interior surface bounding a compartment. An impeller is disposed within the chamber of the flexible bag. A drive shaft is coupled with the impeller such that rotation of the drive shaft facilitates rotation of the impeller. A drive motor assembly is coupled with the draft shaft and is adapted to rotate the drive shaft. An adjustable arm assembly is coupled with the drive motor assembly and is adapted to move the drive motor assembly which in turn moves the position of the drive shaft and impeller. An electrical controller can control movement of the adjustable arm.