The Hetero Ring Has Eight Or More Ring Members And Only Oxygen As Ring Hetero Atoms (e.g., Erythromycin, Spiramycin, Nystatin, Etc.) Patents (Class 435/76)
  • Patent number: 10306887
    Abstract: Embodiments of the invention are directed of compositions for activation or inhibition of the Oct?2 receptor and uses thereof. The inventors have discovered that the beta adrenergic-like octopamine receptor Oct?2 serves as a key signaling molecule for ovulation and recruits protein kinase A and Ca2+/calmodulin-sensitive kinase II as downstream effectors for this activity. Oct?2R homozygous mutant females are sterile, and display normal courtship, copulation, sperm storage and post-mating rejection behavior but were unable to lay eggs.
    Type: Grant
    Filed: August 4, 2015
    Date of Patent: June 4, 2019
    Assignee: THE BOARD OF REGENTS OF THE UNIVERSITY OF TEXAS SYSTEMS
    Inventor: Kyung-an Han
  • Patent number: 9624270
    Abstract: Occidiofungin is a cyclic nonribosomally synthesized antifungal peptide with submicromolar activity. This invention is directed to compositions enriched for particular occidiofungin diastereomers/conformers, methods of making compositions enriched for particular diastereomers/conformers and microorganisms suitable for producing enriched compositions of particular diastereomers/conformers. Methods of treating fungal infections or plants infected by fungi are also provided.
    Type: Grant
    Filed: November 26, 2013
    Date of Patent: April 18, 2017
    Assignees: MISSISSIPPI STATE UNIVERSITY, THE TEXAS A&M UNIVERSITY SYSTEM
    Inventors: James Leif Smith, Akshaya Ravichandran, Shien Lu, Ganyu Gu
  • Patent number: 9394528
    Abstract: The present invention relates to 25 hitherto undescribed genes of B. licheniformis and gene products derived therefrom and all sufficiently homologous nucleic acids and proteins thereof. They occur in five different metabolic pathways for the formation of odorous substances. The metabolic pathways in question are for the synthesis of: 1) isovalerian acid (as part of the catabolism of leucine), 2) 2-methylbutyric acid and/or isobutyric acid (as part of the catabolism of valine and/or isoleucine), 3) butanol and/or butyric acid (as part of the metabolism of butyric acid), 4) propyl acid (as part of the metabolism of propionate) and/or 5) cadaverine and/or putrescine (as parts of the catabolism of lysine and/or arginine).
    Type: Grant
    Filed: December 27, 2006
    Date of Patent: July 19, 2016
    Assignee: BASF SE
    Inventors: Cornelius Bessler, Jörg Feesche, Stefan Evers, Karl-Heinz Maurer, Armin Ehrenreich, Birgit Veith, Heiko Liesegang, Anke Henne, Christina Herzberg, Gerhard Gottschalk
  • Patent number: 9040259
    Abstract: The present invention relates to production of polyketides and other natural products and to libraries of compounds and individual novel compounds. One important area is the isolation and potential use of novel FKBP-ligand analogs and host cells that produce these compounds. The invention is particularly concerned with methods for the efficient transformation of strains that produce FKBP analogs and recombinant cells in which cloned genes or gene cassettes are expressed to generate novel compounds such as polyketide (especially rapamycin) FKBP-ligand analogs, and to processes for their preparation, and to means employed therein (e.g. nucleic acids, vectors, gene cassettes and genetically modified strains).
    Type: Grant
    Filed: March 11, 2011
    Date of Patent: May 26, 2015
    Assignee: Buck Institute for Research on Aging
    Inventors: Matthew Alan Gregory, Sabine Gaisser, Hrvoje Petkovic, Steven Moss
  • Publication number: 20150141294
    Abstract: The present invention relates to methods of use of glycosyltransferases and related novel compounds. The invention exploits the reversibility of glycosyltransferases to generate new sugars, unnatural biomolecules and numerous one-pot reactions for generation of new biomolecules having varied backbones such as enediynes, vancomycins, bleomycins, anthracyclines, macrolides, pluramycins, aureolic acids, indolocarbazoles, aminglycosides, glycopeptides, polyenes, coumarins, benzoisochromanequinones, calicheamicins, erythromycin, avermectins, ivermectins, angucyclines, cardiac glycosides, steroids or flavinoids. In preferred embodiments, the invention specifically relates to biosynthesis of anticancer (the enediyne calicheamicin, CLM), anthelmintic agents (the macrolides avermectin, ivermectin and erythromycin) and antibiotic (the glycopeptide vancomycin, VCM) natural product-based drugs developed by reversible, bidirectional glycosyltransferase-catalyzed reactions.
    Type: Application
    Filed: July 18, 2014
    Publication date: May 21, 2015
    Inventors: JON S. THORSON, CHANGSHENG ZHANG, BYRON R. GRIFFITH
  • Publication number: 20150056651
    Abstract: This invention relates to metabolically engineered microorganism strains, such as bacterial strains, in which there is an increased utilization of malonyl-CoA for production of a chemical product, which includes polyketides and 3-hydroxypropionic acid.
    Type: Application
    Filed: April 7, 2014
    Publication date: February 26, 2015
    Inventors: Michael D. Lynch, Ryan T. Gill, Tanya E.W. Lipscomb
  • Publication number: 20140371436
    Abstract: The present invention relates to a novel polyene compound similar to nystatin, a method for preparing the same, and an antifungal drug comprising the novel polyene compound as an active ingredient. Compared to nystatin comprising one sugar, NNP, which is the polyene compound similar to nystatin, displays 300 times higher solubility and ten times lower cytotoxicity while maintaining antifungal activity, and thus can be useful in developing a novel polyene antifungal agent having improved solubility and less toxicity in terms of pharmacokinetics.
    Type: Application
    Filed: August 31, 2012
    Publication date: December 18, 2014
    Applicant: INHA-INDUSTRY PARTNERSHIP INSTITUTE
    Inventors: Eung-Soo Kim, Kyu Boem Han, Shuangjun Lin, Dekun Kong, Linquan Bai, Zixin Deng, David H. Sherman, Mi-Jin Lee
  • Patent number: 8911970
    Abstract: Fermentation media containing an isoflavone-depleted soybean meal or isoflavone-depleted soybean meal product and at least one exogenous added ingredient that comprises a substrate for microbial growth are provided. Methods of making a fermentation medium comprising an isoflavone-depleted soybean meal or isoflavone-depleted soybean meal product and methods for obtaining a fermentation product are also provided. The present invention is further directed to fermentation broths obtained by the media and methods. The present invention is also directed to feed additives produced from fermentation broths obtained by the methods.
    Type: Grant
    Filed: March 19, 2013
    Date of Patent: December 16, 2014
    Assignee: Fermalogic, Inc.
    Inventor: J. Mark Weber
  • Publication number: 20140248667
    Abstract: The invention describes the integration of polynucleotides into chromosomal DNA of S. spinosa species, which are useful for the production of insecticides, integrants thereof, and also to the use of the integrants. The invention includes the stable integration and expression of an oxygen-binding protein, VHb, which results in increased spinosyn production.
    Type: Application
    Filed: May 16, 2014
    Publication date: September 4, 2014
    Inventors: Lei Han, Nigel Mouncey
  • Patent number: 8778896
    Abstract: Disclosed are levoisovalerylspiramycin I, II or III, preparations, preparing methods and uses thereof. The preparations comprise levoisovalerylspiramycin I, II or III and pharmaceutically acceptable carrier and/or adjuvant, wherein the purity of levoisovalerylspiramycin I, II or III is above 90 wt %. The levoisovalerylspiramycin I, II or III has a good antibacterial activity, and the preparations include solution for injection, powder for injection or lyophilized powder for injection.
    Type: Grant
    Filed: May 25, 2011
    Date of Patent: July 15, 2014
    Assignee: Shenyang Tonglian Group Co., Ltd.
    Inventors: Yang Jiang, Yuyou Hao
  • Patent number: 8759031
    Abstract: Novel extender units for Type I polyketide synthases are provided. Also provided are genes, compounds, and methods for generating these units, and for incorporation of the novel extender units into polyketides for the purpose of generating new structural derivatives of polyketide-containing products.
    Type: Grant
    Filed: April 9, 2008
    Date of Patent: June 24, 2014
    Assignee: Wisconsin Alumni Research Foundation
    Inventors: Michael G. Thomas, Jo Handelsman, Yolande Alia Chan, Angela M. Podevels
  • Patent number: 8759032
    Abstract: A genetically engineered strain WSJ-IA for producing isovaleryl spiramycin I. Also provided is a method for preparing the strain, including the steps of: (a) constructing a recombinant plasmid including a double gene ist-acyB2; (b) transforming the plasmid into an isovaleryl spiramycin I-producing strain to obtain the strain WSJ-IA. The level of isovaleryl spiramycin I produced by fermentation of the strain WSJ-IA is increased 1.7 times and the fermentation potency thereof increased 4.14 times in comparison with the strain exclusively including a single gene ist.
    Type: Grant
    Filed: July 21, 2011
    Date of Patent: June 24, 2014
    Assignee: Shen Yang Tonglian Group Co., Ltd.
    Inventors: Yiguang Wang, Yang Jiang, Shengwu Yang, Xiaofeng Zhao
  • Patent number: 8741603
    Abstract: The invention describes the integration of polynucleotides into chromosomal DNA of S. spinosa species, which are useful for the production of insecticides, integrants thereof, and also to the use of the integrants. The invention includes the stable integration and expression of an oxygen-binding protein, VHb, which results in increased spinosyn production.
    Type: Grant
    Filed: May 3, 2011
    Date of Patent: June 3, 2014
    Assignee: Agrigenetics Inc.
    Inventors: Lei Han, Nigel Mouncey
  • Publication number: 20140134672
    Abstract: Bioreactors, and particularly, photobioreactors having a reactor chamber and surge driver, and methods for using these devices, for example, for the production of carbon-based products are provided. The reactor chamber provides a housing for microorganisms and culture medium. The surge driver produces a surge of the microorganisms and/or culture medium in the reactor chamber.
    Type: Application
    Filed: July 5, 2012
    Publication date: May 15, 2014
    Inventors: Max B. Tuttman, David A. St. Angelo
  • Patent number: 8664186
    Abstract: The present invention relates to Stambomycin compounds, their derivatives and their pharmaceutically acceptable salt thereof.
    Type: Grant
    Filed: July 23, 2010
    Date of Patent: March 4, 2014
    Assignees: Universite de Lorraine, University of Warwick, Institut National de la Recherche Agronomique
    Inventors: Bertrand Aigle, Gregory Challis, Luisa Laureti, Lijiang Song, Pierre Leblond
  • Patent number: 8624009
    Abstract: The invention provides, biologically active spinosyns, hybrid spinosyn polyketide synthases capable of functioning in Saccharopolyspora spinosa to produce the spinosyns, and methods of controlling insects using the spinosyns.
    Type: Grant
    Filed: March 28, 2011
    Date of Patent: January 7, 2014
    Assignee: Dow AgroSciences, LLC.
    Inventors: Lesley S. Burns, Paul R. Graupner, Paul Lewer, Christine J. Martin, William A. Vousden, Clive Waldron, Barrie Wilkinson
  • Publication number: 20130288308
    Abstract: Fermentation media containing an isoflavone-depleted soybean meal or isoflavone-depleted soybean meal product and at least one exogenous added ingredient that comprises a substrate for microbial growth are provided. Methods of making a fermentation medium comprising an isoflavone-depleted soybean meal or isoflavone-depleted soybean meal product and methods for obtaining a fermentation product are also provided. The present invention is further directed to fermentation broths obtained by the media and methods. The present invention is also directed to feed additives produced from fermentation broths obtained by the methods.
    Type: Application
    Filed: March 19, 2013
    Publication date: October 31, 2013
    Inventor: J. Mark Weber
  • Publication number: 20130266986
    Abstract: The present invention relates to a process for the production of tiacumicin B which comprises fermentation of a micro-organism of the species Dactylosporangium aurantiacum in a culture broth containing polysaccharides such as cellulose and derivatives thereof.
    Type: Application
    Filed: April 3, 2013
    Publication date: October 10, 2013
    Applicant: OLON S.p.A.
    Inventors: Antonella Malcangi, Guido Trione
  • Publication number: 20130172215
    Abstract: This disclosure concerns methods for producing a spinosyn producing strain that comprises modifying a nucleic acid molecule encoding for spinactin by introducing, mutating, deleting, replacing or inactivating a nucleic acid sequence encoding one or more activities encoded by said nucleic acid molecule. Methods for producing a modified Saccharopolyspora spinosa organism are also disclosed.
    Type: Application
    Filed: December 13, 2012
    Publication date: July 4, 2013
    Applicant: DOW AGROSCIENCES LLC
    Inventor: DOW AGROSCIENCES LLC
  • Publication number: 20130150316
    Abstract: The present invention relates to levocarrimycin, its pharmaceutical compositions, preparation methods and application. Levocarrimycin is a mixture of isovalerylspiramycin III, II and I as main components and contains some isobutyrylspiramycin III and II, butyrylspiramycin III and II, propionylspiramycin III and II, as well as acetylspiramycin III and II, among which, the content of isovalerylspiramycin III is no less than 30 wt %, the total content of isovalerylspiramycin III, II and I is no less than 60 wt %, and the content of acylspiramycin is 80-98 wt %. Specific optical rotation of said levocarrimycin is [?]D=?52°˜?57° in the solution of 0.02 g/ml chloroform at temperature of 25° C. The present invention also relates to the crystalline compound of isovalerylspiramycin III, II or I in levocarrimycin, and pharmaceutical compositions containing the said levocarrimycin.
    Type: Application
    Filed: May 25, 2011
    Publication date: June 13, 2013
    Applicant: Shenyang Toglian Group Co., Ltd.
    Inventors: Yang Jiang, Yuyou Hao
  • Publication number: 20130123477
    Abstract: Methods, processes and materials for the production and recovery of Tiacumicins produced by culturing a microorganism belonging to the species Dactylosporangium aurantiacum subspecies hamdenensis having the ability to produce and accumulate one or more Tiacumicin in a nutrient medium comprising a carbon source, a nitrogen source, trace elements such as inorganic salts, and an adsorbent, wherein said nitrogen source comprises fish powder, and wherein said Tiacumicin is produced in a yield greater than about 50 mg/L broth.
    Type: Application
    Filed: June 12, 2012
    Publication date: May 16, 2013
    Applicant: OPTIMER PHARMACEUTICALS, INC.
    Inventors: Youe-Kong Shue, Chi-Jen Frank Du, Ming-His Chiou, Mei-Chiao Wu, Yuan-Ting Chen, Franklin W. Okumu, Jonathan James Duffield
  • Publication number: 20130122541
    Abstract: This invention relates to metabolically engineered microorganism strains, such as bacterial strains, in which there is an increased utilization of malonyl-CoA for production of a chemical product, which includes polyketides and 3-hydroxypropionic acid.
    Type: Application
    Filed: January 27, 2011
    Publication date: May 16, 2013
    Inventors: Michael D. Lynch, Ryan T. Gill, Tanya E.W. Lipscomb
  • Publication number: 20130065848
    Abstract: Disclosed are levoisovalerylspiramycin I, II or III, preparations, preparing methods and uses thereof. The preparations comprise levoisovalerylspiramycin I, II or III and pharmaceutically acceptable carrier and/or adjuvant, wherein the purity of levoisovalerylspiramycin I, II or III is above 90 wt %. The levoisovalerylspiramycin I, II or III has a good antibacterial activity, and the preparations include solution for injection, powder for injection or lyophilized powder for injection.
    Type: Application
    Filed: May 25, 2011
    Publication date: March 14, 2013
    Applicant: Shenyang Tonglian Group Co., Ltd.
    Inventors: Yang Jiang, Yuyou Hao
  • Patent number: 8318684
    Abstract: Seven novel antibiotic substances can be produced by cultivation of a microbial strain which has been isolated from a soil sample and which is designated as Microbispora sp. A 34030 (deposited under an access number FERM BP-10505 in terms of Budapest Treaty). These seven antibiotic substances are named as bispolide A1, bispolide A2, bispolide A3, bispolide B1, bispolide B2a, bispolide B2b and bispolide B3, respectively. These bispolides are each novel compounds which have a chemical structure as collectively represented by the general formula (III) shown below: These bispolides have each a high antibacterial activity against a variety of bacteria, particularly Gram-positive bacteria and their antibiotic-resistant strains, and hence these bispolides each are effective and useful for therapeutically treating bacterial infections of Gram-positive bacteria in humans and animals.
    Type: Grant
    Filed: May 15, 2007
    Date of Patent: November 27, 2012
    Assignees: Nimura Genetic Solutions Co., Ltd., Forest Research Institute Malaysia
    Inventors: Keiichi Numata, Noriyuki Okujo, Siew Eim Khor, Lee Li Tan, Annie George, Szu Ting Ng, Chin Jye Tan, Hironobu Iinuma, Yasuo Fukagawa, Kunimoto Hotta, Seiji Shibahara, Shinichi Kondo, Satoshi Nimura
  • Publication number: 20120282657
    Abstract: The invention describes the integration of polynucleotides into chromosomal DNA of S. spinosa species, which are useful for the production of insecticides, integrants thereof, and also to the use of the integrants. The invention includes the stable integration and expression of an oxygen-binding protein, VHb, which results in increased spinosyn production.
    Type: Application
    Filed: May 3, 2011
    Publication date: November 8, 2012
    Applicant: DOW AGROSCIENCES LLC
    Inventors: Lei Han, Nigel Mouncey
  • Publication number: 20120190635
    Abstract: The present invention provides regio- and stereoselective oxidation of unactivated C—H bonds using an engineered mutant cytochrome P450 monooxygenase and an engineered substrate.
    Type: Application
    Filed: September 27, 2010
    Publication date: July 26, 2012
    Applicant: THE REGENTS OF THE UNIVERSITY OF MICHIGAN
    Inventors: Shengying Li, David H. Sherman, John Montgomery, Mani R. Chaulagain, Allison R. Knauff
  • Publication number: 20120142621
    Abstract: A microbial ethyl esther sophorolipid derivative with no acetylated groups produced by Candida species, for treating and preventing sepsis/septic shock. The method of producing sophorolipids is through microbial resting cells of Candida bombicola. The sophorolipids obtained from resting state cultures are isolated as a complex mixture of compounds and then decanted as a dense oil from the culture broth, subsequently washed to remove free fatty acids. Secondary chemical transformation via base catalyzed hydrolysis is used to reduce the 8 possible structural sophorolipid species to a single moiety, the 17-L-[(2?-O-b-D-glucopyranosyl-b-D-glucopyranosyl)-oxy]-cis-9-octadecenoate de-acetylated free acid. The compound acts primarily through decreasing inflammatory cytokines and eliciting other synergistic anti-inflammatory mechanisms by blocking TLR4-CD14 upstream of the inflammatory signaling cascade.
    Type: Application
    Filed: August 2, 2011
    Publication date: June 7, 2012
    Applicant: Biomedica Management Corporation
    Inventors: George Falus, Maja Nowakowski, Martin Bluth, John Aikens
  • Publication number: 20120142622
    Abstract: The present invention relates to Stambomycin compounds, their derivatives and their pharmaceutically acceptable salt thereof.
    Type: Application
    Filed: July 23, 2010
    Publication date: June 7, 2012
    Applicants: UNIVERSITE HENRI POINCARE NANCY 1, INSTITUT NATIONAL DE LA RECHERCHE AGRONOMIQUE, UNIVERSITY OF WARWICK
    Inventors: Bertrand Aigle, Gregory Challis, Luisa Laureti, Lijiang Song, Pierre Leblond
  • Patent number: 8188245
    Abstract: This disclosure describes the molecular cloning of an enduracidin biosynthetic gene cluster from Streptomyces fungicidicus, and characterization of individual genes in the gene cluster and the proteins encoded thereby. An enduracidin gene cluster is located within a 116 kilobases genetic locus and includes 25 open reading frames (ORFs). An additional 23 ORFs flank the disclosed enduracidin biosynthetic gene cluster. Enduracidin analogs and a method for producing them by manipulation of the enduracidin gene cluster and specific genes therein are also disclosed.
    Type: Grant
    Filed: October 1, 2007
    Date of Patent: May 29, 2012
    Assignee: State of Oregon acting by and through the State Board of Higher Education on behalf of Oregon State University
    Inventors: Xihou Yin, T. Mark Zabriskie
  • Patent number: 8143035
    Abstract: The invention relates to the synthesis of polyketides in Actinomycetes by overexpression of pantothenate kinase and supplementation with pantothenic acid. This results in increasing in vivo CoA production and thereby drives increased production of secondary metabolites, such as polyketides.
    Type: Grant
    Filed: September 22, 2006
    Date of Patent: March 27, 2012
    Assignee: Rice University
    Inventors: Hei Chan Lee, Jae Kyung Sohng, Ka-Yiu San
  • Publication number: 20110262971
    Abstract: Genetically modified E. coli strains capable of producing erythromycins, particularly erythromycin A.
    Type: Application
    Filed: April 21, 2010
    Publication date: October 27, 2011
    Applicant: Tufts University
    Inventors: Blaine Pfeifer, Haoran Zhang
  • Patent number: 8044186
    Abstract: Provided are nucleic acid molecules comprising at least a functional fragment of the capreomycin biosynthetic gene cluster, polypeptides encoded by the cluster and recombinant host cells transformed with any of the nucleic acid molecules disclosed herein. Various methods using any of the vectors or expression cassettes that encode one or more of the gene products of the cluster are provided for heterologous production of capreomycin and capreomycin derivatives.
    Type: Grant
    Filed: May 9, 2008
    Date of Patent: October 25, 2011
    Assignee: Wisconsin Alumni Research Foundation
    Inventors: Michael George Thomas, Elizabeth Anne Felnagle, Michelle Renee Rondon, Andrew David Berti
  • Patent number: 8008078
    Abstract: The present invention relates to polynucleotide molecules comprising nucleotide sequences encoding an aveC gene product, which polynucleotide molecules can be used to alter the ratio or amount of class 2:1 avermectins produced in fermentation cultures of S. avermitilis. The present invention further relates to vectors, host cells, and mutant strains of S. avermitilis in which the aveC gene has been inactivated, or mutated so as to change the ratio or amount of class 2:1 avermectins produced.
    Type: Grant
    Filed: April 23, 2008
    Date of Patent: August 30, 2011
    Assignees: Pfizer Inc., Pfizer Products Inc.
    Inventors: Kim J. Stutzman-Engwall, Anke Krebber, Claes Gustafsson, Jeremy S. Minshull, Sun Ai Raillard, Seran Kim, Yan Chen
  • Publication number: 20110178036
    Abstract: The invention provides, biologically active spinosyns, hybrid spinosyn polyketide synthases capable of functioning in Saccharopolyspora spinosa to produce the spinosyns, and methods of controlling insects using the spinosyns.
    Type: Application
    Filed: March 28, 2011
    Publication date: July 21, 2011
    Inventors: Lesley S. Burns, Paul R. Graupner, Paul Lewer, Christine J. Martin, William A. Vousden, Clive Waldron, Barrie Wilkinson
  • Publication number: 20100304441
    Abstract: The present invention relates to fungal strains capable of producing insecticide and a process for production of insecticide. It also relates to a method of cultivation of fungal strains and a fermentation medium for culturing the fungal strains.
    Type: Application
    Filed: October 22, 2008
    Publication date: December 2, 2010
    Inventors: Ram Rajasekharan, Chikkarasanahalli Shivegowda Vivekbabu
  • Patent number: 7714118
    Abstract: Host cells comprising recombinant vectors encoding the FK-520 polyketide synthase and FK-520 modification enzymes can be used to produce the FK-520 polyketide. Recombinant DNA constructs comprising one or more FK-520 polyketide synthase domains, modules, open reading frames, and variants thereof can be used to produce recombinant polyketide synthases and a variety of different polyketides with application as pharmaceutical and veterinary products.
    Type: Grant
    Filed: July 6, 2004
    Date of Patent: May 11, 2010
    Assignee: Kosan Biosciences Incorporated
    Inventors: Christopher Reeves, Kai Wu
  • Patent number: 7709231
    Abstract: The invention provides a nucleic acid molecule comprising: (a) a nucleotide sequence as shown in SEQ ID No. 35; or (b) a nucleotide sequence which is the complement of SEQ ID No. 35; or (c) a nucleotide sequence which is degenerate with SEQ ID No. 35; or (d) a nucleotide sequence hybridising under conditions of high stringency to SEQ ID No. 35, to the complement of SEQ ID No. 35, or to a hybridisation probe derived from SEQ ID No. 35 or the complement thereof; or (e) a nucleotide sequence having at least 80% sequence identity with SEQ ID No. 35; or (f) a nucleotide sequence having at least 65% sequence identity with SEQ ID No. 35 wherein said sequence preferably encodes or is complementary to a sequence encoding a nystatin PKS enzyme or a part thereof.
    Type: Grant
    Filed: September 11, 2006
    Date of Patent: May 4, 2010
    Assignee: Sinvent AS
    Inventors: Sergey Borisovich Zotchev, Olga Nikolayivna Sekurova, Espen Fjærvik, Trygve Brautaset, Arne Reidar Strøm, Svein Valla, Trond Erling Ellingsen, Håvard Sletta, Ole-Martin Gulliksen
  • Patent number: 7680601
    Abstract: Methods for the computational analysis of polyketides and the computer-assisted design of PKS genes are facilitated by representing the structure of a polyketide and/or a PKS gene that encodes the PKS that produces the polyketide by alphanumeric symbols that facilitates computer assisted analysis. A database of polyketides and corresponding PKS genes that can be rapidly searched and information extracted for a variety of applications, including the design and specification of PKS genes via the recombining of modules or portions of modules or sets of modules from already known and available PKS genes.
    Type: Grant
    Filed: May 29, 2001
    Date of Patent: March 16, 2010
    Assignee: Kosan Biosciences
    Inventors: Chaitan Khosla, Ralph C. Reid, Daniel V. Santi, Michael A. Siani
  • Patent number: 7655442
    Abstract: The present invention provides a method for stabilizing a macrolide compound, and an efficient method for producing the compound. Specifically, it provides a method for stabilizing a macrolide compound, in which a 12-membered ring macrolide compound, such as a compound expressed by the formula (1) and a cyclodextrin are both present, and a method for producing a macrolide compound, in which a cyclodextrin is made to be present in a culture broth of actinomycetes having an ability of producing the macrolide compound.
    Type: Grant
    Filed: January 28, 2005
    Date of Patent: February 2, 2010
    Assignees: Eisai R&D Management Co., Ltd., Mercian Corporation
    Inventors: Hiroshi Ishihara, Susumu Takeda, Tomonari Yamada, Yoshiaki Asahi
  • Patent number: 7638306
    Abstract: A process of increasing the cellular production of biologically active compounds is provided. The process is particularly useful for increasing antibiotic production by bacterial cells. The process includes the step of inhibiting the activity of methylmalonyl-CoA mutase.
    Type: Grant
    Filed: August 8, 2003
    Date of Patent: December 29, 2009
    Assignee: Fermalogic, Inc.
    Inventors: J. Mark Weber, Andrew R. Reeves, Igor A. Brikun, William Henry Cernota
  • Patent number: 7626010
    Abstract: The invention provides, biologically active spinosyns, hybrid spinosyn polyketide synthases capable of functioning in Saccharopolyspora spinosa to produce the spinosyns, and methods of controlling insects using the spinosyns.
    Type: Grant
    Filed: October 20, 2005
    Date of Patent: December 1, 2009
    Assignee: Dow AgroSciences LLC
    Inventors: Lesley S. Burns, Paul R. Graupner, Paul Lewer, Christine J. Martin, William A. Vousden, Clive Waldron, Barrie Wilkinson
  • Patent number: 7595156
    Abstract: The present invention relates to the base sequence of whole genes involved in the biosynthesis of FR-008 polyketides derived from Streptomyces sp. FR-008. This base sequence comprises genes coding for ketosynthase (KS), acyl transferase (AT), acyl carrier protein (ACP), ketoreductase (KR), dehydratase (DH) and enoyl reductase (ER) domains, and genes coding for modifier enzymes, such as ABC transporter, cytochrome P450 monooxygenase, ferredoxin, thioesterase, sugar synthetic protein, FAD-dependent monooxygenase, 4-amino-4-deoxychorismate (ADC) synthase and ADC lyase. The gene base sequence according to the present invention can be used to increase the productivity of the existing FR-008 polyketides or produce new FR-008 polyketides, through modification of its parts.
    Type: Grant
    Filed: April 6, 2004
    Date of Patent: September 29, 2009
    Assignee: Korea Advanced Institute of Science and Technology
    Inventors: Sang Yup Lee, Zixin Deng, Shi Chen, Ki Jun Jeong, Xiufen Zhou
  • Publication number: 20090221520
    Abstract: The invention relates to novel polyenes having formula (I), wherein: R1 represents alkyl C1-C3; and R2 represents a functional group selected from CH3— or CONH2— (methyl- or primary amide-). The aforementioned polyenes have a biocide action on organisms comprising cell membranes that contain ergosterol, e.g., fungi or parasites. Said compounds can be obtained using a method that consists in cultivating a producing micro-organism under conditions that enable the production thereof. In addition, the invention also relates to a mechanism for the in vitro production of amidated polyenes, consisting in incubating carboxylated polyenes with cell-free extracts (or proteinaceous fractions) of the producers of same in the presence of ATP/Mg++ and an amide- group donor compound (preferably glutamine).
    Type: Application
    Filed: March 23, 2006
    Publication date: September 3, 2009
    Inventors: Francisco Malpartida Romero, Elena Maria Seco Martin, Trinidad Cuesta Velasco
  • Patent number: 7560252
    Abstract: The present invention relates to the biosynthesis of polyketides and derives from the cloning of nucleic acids encoding a polyketide synthase and other associated proteins involved in the synthesis of the polyketide borrelidin. Materials and methods including enzyme systems, nucleic acids, vectors and cells are provided for the preparation of polyketides including borrelidin and analogues and derivatives thereof. Novel polyketide molecules are also provided.
    Type: Grant
    Filed: December 24, 2003
    Date of Patent: July 14, 2009
    Assignees: Biotica Technology Limited, The University of Oviedo
    Inventors: Jose A Salas, Carmen Mendez, Carlos Olano, Cesar Sanchez, Alfredo F Brana, Barrie Wilkinson, Christine J Martin, Steven Moss, Peter F Leadlay, Marko Oliynyk
  • Patent number: 7521414
    Abstract: A novel family of cyclic polyene natural products isolated from marine actinomycete strain CNQ140 is provided. This novel strain of actinomycetes was obtained from a previously unstudied population of marine actinomycetes that reside in sediments off La Jolla, Calif. Compounds derived from strain CNQ140 have been characterized as having a cyclic polyene-polyol structure; a molecular weight from about 996 to about 1010 in the core ring structure; and at least 58 carbons and at least 14 oxygens. The invention compounds have antitumor and/or anti-microbial activity.
    Type: Grant
    Filed: June 21, 2004
    Date of Patent: April 21, 2009
    Assignee: The Regents of the University of California
    Inventors: William H. Fenical, Paul R. Jensen, Hak Cheol Kwon
  • Patent number: 7459294
    Abstract: During the production of a product compound by fermentation, the concentration of a precursor compound is maintained within a pre-selected concentration range by having an adsorbent resin in contact with the culture medium. The adsorbent resin reversibly adsorbs precursor compound and, as un-adsorbed precursor compound is converted to product compound, adsorbed precursor compound is released from the resin, thus maintaining the concentration of precursor compound within the pre-selected range.
    Type: Grant
    Filed: August 6, 2004
    Date of Patent: December 2, 2008
    Assignee: Kosan Biosciences Incorporated
    Inventors: Timothy A. Leaf, Ruchir P. Desai, Peter Licari, Elaine Jennifer Woo
  • Patent number: 7413878
    Abstract: Host cells, such as E. coli, are provided with an expression system for making starter units required for biosynthesis of polyketides using the ato pathway.
    Type: Grant
    Filed: July 15, 2003
    Date of Patent: August 19, 2008
    Assignee: Kosan Biosciences, Inc.
    Inventors: James T. Kealey, Linda C. Dayem, Daniel V. Santi
  • Patent number: 7388085
    Abstract: The present invention relates to polynucleotide molecules comprising nucleotide sequences encoding an aveC gene product, which polynucleotide molecules can be used to alter the ratio or amount of class 2:1 avermectins produced in fermentation cultures of S. avermitilis. The present invention further relates to vectors, host cells, and mutant strains of S. avermitilis in which the aveC gene has been inactivated, or mutated so as to change the ratio or amount of class 2:1 avermectins produced.
    Type: Grant
    Filed: February 10, 2003
    Date of Patent: June 17, 2008
    Assignee: Pfizer Inc
    Inventors: Kim J. Stutzman-Engwall, Anke Krebber, Claes Gustafsson, Jeremy S. Minshull, Sun Ai Raillard, Seran Kim, Yan Chen
  • Patent number: 7381546
    Abstract: The rapamycin gene cluster is an example of a gene cluster which includes a gene (rapL) leading to the formation of a precursor compound (pipecolic acid, in this case) which is required for inclusion in the larger product (rapamycin) produced by the enzymes encoded by the cluster. We have produced a mutant strain containing a rapamycin gene cluster in which the rapL gene is disabled. The strain does not naturally produce rapamycin but does so if fed with pipecolic acid. By feeding with alternative carboxylic acids we have produced variants of rapamycins. Tests have shown biological activity.
    Type: Grant
    Filed: January 10, 2006
    Date of Patent: June 3, 2008
    Assignee: Biotica Technology Limited
    Inventors: Peter Francis Leadlay, James Staunton, Lake Ee Khaw
  • Patent number: 7291486
    Abstract: Recombinant Escherichia coli host cells that comprise recombinant DNA expression vectors that drive expression of methylmalonyl CoA mutase from Propionibacterium shermanii or Streptomyces cinnamonensis as well as Propionibacterium shermanii epimerase can produce S-methylmalonyl CoA, a required substrate for the production of polyketides by most modular polyketide synthases not present in wild-type E. coli host cells.
    Type: Grant
    Filed: April 21, 2004
    Date of Patent: November 6, 2007
    Assignee: KOSAN Biosciences, Inc.
    Inventors: Daniel Santi, Linda Dayem, James Kealey