The Hetero Ring Has Eight Or More Ring Members And Only Oxygen As Ring Hetero Atoms (e.g., Erythromycin, Spiramycin, Nystatin, Etc.) Patents (Class 435/76)
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Patent number: 7388085Abstract: The present invention relates to polynucleotide molecules comprising nucleotide sequences encoding an aveC gene product, which polynucleotide molecules can be used to alter the ratio or amount of class 2:1 avermectins produced in fermentation cultures of S. avermitilis. The present invention further relates to vectors, host cells, and mutant strains of S. avermitilis in which the aveC gene has been inactivated, or mutated so as to change the ratio or amount of class 2:1 avermectins produced.Type: GrantFiled: February 10, 2003Date of Patent: June 17, 2008Assignee: Pfizer IncInventors: Kim J. Stutzman-Engwall, Anke Krebber, Claes Gustafsson, Jeremy S. Minshull, Sun Ai Raillard, Seran Kim, Yan Chen
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Patent number: 7381546Abstract: The rapamycin gene cluster is an example of a gene cluster which includes a gene (rapL) leading to the formation of a precursor compound (pipecolic acid, in this case) which is required for inclusion in the larger product (rapamycin) produced by the enzymes encoded by the cluster. We have produced a mutant strain containing a rapamycin gene cluster in which the rapL gene is disabled. The strain does not naturally produce rapamycin but does so if fed with pipecolic acid. By feeding with alternative carboxylic acids we have produced variants of rapamycins. Tests have shown biological activity.Type: GrantFiled: January 10, 2006Date of Patent: June 3, 2008Assignee: Biotica Technology LimitedInventors: Peter Francis Leadlay, James Staunton, Lake Ee Khaw
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Genetically modified cells and methods for converting (R)-methylmalonyl CoA to (S)-methylmalonyl CoA
Patent number: 7291486Abstract: Recombinant Escherichia coli host cells that comprise recombinant DNA expression vectors that drive expression of methylmalonyl CoA mutase from Propionibacterium shermanii or Streptomyces cinnamonensis as well as Propionibacterium shermanii epimerase can produce S-methylmalonyl CoA, a required substrate for the production of polyketides by most modular polyketide synthases not present in wild-type E. coli host cells.Type: GrantFiled: April 21, 2004Date of Patent: November 6, 2007Assignee: KOSAN Biosciences, Inc.Inventors: Daniel Santi, Linda Dayem, James Kealey -
Patent number: 7285653Abstract: The present invention relates to nucleic acid coding for enzyme activities of spinosyn biosynthesis and to the relevant enzymes per se. Furthermore, the invention relates to methods for preparing spinsoyn derivatives and spinosyn precursors.Type: GrantFiled: August 17, 2000Date of Patent: October 23, 2007Assignee: Bayer AktiengesellschaftInventors: Günther Eberz, Volker Möhrle, Rita Fröde, Robert Velten, José A. Salas
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Patent number: 7220560Abstract: The present invention provides a generalized oxygen-limited cultivation method for myxobacterial strains engineered to heterologously express polyketides synthase (PKS) gene clusters under various oxygen tension conditions, modulating the polyketide congener distribution.Type: GrantFiled: February 25, 2003Date of Patent: May 22, 2007Assignee: Kosan Biosciences IncorporatedInventors: Peter J. Licari, Bryan Julien, Scott Frykman, Hiroko Tsuruta
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Patent number: 7208294Abstract: A display device and a display panel driving method, in which a matrix panel includes pixel portions each have a series circuit of a bistable element and a light emitting element, every time one scan line is specified in order in accordance with an input image signal, a driving line corresponding to at least one pixel portion to be driven to emit light on the one scan line is specified in accordance with the input image signal, a first predetermined voltage lower than a turn-off threshold voltage is applied between the one scan line and the specified driving line, and thereafter a second predetermined voltage higher than a turn-on threshold voltage is applied therebetween.Type: GrantFiled: March 12, 2004Date of Patent: April 24, 2007Assignee: Pioneer CorporationInventor: Takahisa Tanabe
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Patent number: 7198922Abstract: A polyketide synthase (“PKS”) of Type I is a complex multienzyme including a loading domain linked to a multiplicity of extension domains. The first extension module receives an acyl starter unit from the loading domain and each extension module adds a further ketide unit which may undergo processing (e.g. reduction). We have found that the Ksq domain possessed by some PKS's has decarboxylating activity, e.g. generating (substituted) acyl from (substituted) malonyl. The CLF domain of type II PKS's has similar activity. By inserting loading modules including such domains into PKS's not normally possessing them it is possible to control the starter units used.Type: GrantFiled: February 19, 2004Date of Patent: April 3, 2007Assignees: Biotica Technology Limited, Pfizer, Inc.Inventors: Peter Francis Leadlay, James Staunton, Jesus Cortes, Hamish Alastair Irvine McArthur
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Patent number: 7067286Abstract: Recombinant host cells of the suborder Cystobacterineae, genus Stizmatella, containing recombinant expression vectors that encode heterologous epothilone polyketide synthase (PKS) genes and can produce epothilones at high levels are provided.Type: GrantFiled: July 8, 2002Date of Patent: June 27, 2006Assignee: Kosan Biosciences, Inc.Inventors: Bryan Julien, Leonard Katz, Chaitan Khosla
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Patent number: 7049100Abstract: A multiple-plasmid system for heterologous expression of polyketides facilitates combinatorial biosynthesis. The method can be extended to any modular polyketide synthase (PKS) or non-ribosomal peptide synthase (NRPS) and has the potential to produce thousands of novel natural products, including ones derived from further modification of the PKS or NRPS products by tailoring enzymes.Type: GrantFiled: March 22, 2002Date of Patent: May 23, 2006Assignee: Kosan Biosciences IncorporatedInventors: Daniel V. Santi, Qun Xue, Gary Ashley
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Patent number: 7033621Abstract: Processes for the production of isoflavones are described wherein plant material from plants of the genus leguminosae are contacted with water, an enzyme which cleaves isoflavone glycosides to the aglucone form and a C2-C10 organic solvent, so as to form a combination, incubating the combination for a time sufficient to allow isoflavones of the aglucone form to partition into the organic solvent component, and thereafter recovering isoflavones from the organic solvent component.Type: GrantFiled: April 11, 2000Date of Patent: April 25, 2006Assignee: Novogen, Inc.Inventors: Graham E. Kelly, Jiu Li Huang, Mark G. Deacon-Shaw, Mark A. Waring
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Patent number: 7018814Abstract: Provided is a method of purifying a vancomycin from a fermentation broth of a microorganism containing vancomycin, including passing the fermentation broth of a microorganism containing vancomycin through a strong acid cation exchange resin, a weak base anion exchange resin, alumina and a hydrophobic absorbent resin sequentially.Type: GrantFiled: September 17, 2004Date of Patent: March 28, 2006Assignee: CJ CorporationInventors: Ju Won Lee, Yun Taek Jung, Jung Woo Suh, Kwang Seob Lee
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Patent number: 7011959Abstract: Recombinant E. coli host cells that comprise recombinant DNA expression vectors that drive expression of methylmalonyl CoA mutase from Propionibacterium shermanii or Streptomyces cinnamonensis as well as Propionibacterium shermanii epimerase can produce S-methylmalonyl CoA, a required substrate for the production of polyketides by most modular polyketide synthases and is not present in wild-type E. coli host cells.Type: GrantFiled: October 27, 2000Date of Patent: March 14, 2006Assignee: Kosan Biosciences, Inc.Inventors: Daniel Santi, Larry Peck, Linda Dayem, James Kealey
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Patent number: 7001747Abstract: Nucleic acid molecules encoding at least part of a Type I polyketide synthase, and having a polylinker with multiple restriction enzyme sites in place of one or more PKS genes encoding enzymes associated with reduction, optionally further including nucleic acid incorporated into the polylinker, the further nucleic acid encoding one or more reductive enzymes; plasmids incorporating such nucleic acids; host cells transfected with such plasmids; methods relating thereto.Type: GrantFiled: July 6, 1999Date of Patent: February 21, 2006Assignees: Biotica Technology Limited, Pfizer, Inc.Inventors: Johannes Kellenberger, Peter Francis Leadlay, James Staunton, Kim Jonelle Stutzman-Engwall, Hamish Alastair Irvine McArthur
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Patent number: 7001748Abstract: Linking sequences which modulate cross-talk between modules of Type I polyketide synthases have been identified. Thus, arbitrarily chosen modules can be mixed and matched by supplying the appropriate linkers to obtain desired polyketide synthases and new polyketides. The modules are provided suitable linkers so that the polyketide chain is passed from one module to the other in the correct sequence. Synthetic peptides which mimic linkers can be used to inhibit the synthesis of polyketides. Kinetic channeling, both intrapolypeptide and interpolypeptide, of diketide intermediates in a Type I polyketide synthase can occur.Type: GrantFiled: March 4, 2002Date of Patent: February 21, 2006Assignee: The Board of Trustees of the Leland Stanford Junior UniversityInventors: Rajesh S. Gokhale, Stuart Tsuji, Chaitan Khosla
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Patent number: 6998256Abstract: Methods are provided for purification of epothilone D and epothilone D analogs from epothilone producing cells such as, for example, Sorangium cellulosum and Myxococcus xanthus (recombinant). Epothilone can be purified from fermentation broth, for example by using resins, chromatography, and crystallization. Epothilone D can be crystallized from a binary solvent system in which water is the forcing solvent.Type: GrantFiled: September 19, 2001Date of Patent: February 14, 2006Assignee: Kosan Biosciences, Inc.Inventor: Robert L. Arslanian
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Patent number: 6984515Abstract: A polyketide or glycosylated polyketide which has the formula: wherein R* is methyl or ethyl; each of R1-R6 is methyl; X1 is OH or H; and/or X2 is=O, OH or H; X3 is OH or H; X4 is OH or H; a pi bond is present at positions 10-11, 8-9, 4-5 and/or 2-3; and wherein at least one of the following characteristics is present: X1 is H; X2 is OH or H; X3 is H; X4 is H; or a pi bond is present at positions 10-11, 8-9, 4-5 and/or 2-3.Type: GrantFiled: May 9, 2001Date of Patent: January 10, 2006Assignees: The LeLand Stanford Junior University, Kosan Biosciences, Inc.Inventors: Chaitan Khosla, Gary Ashley, Camilla Kao, Robert McDaniel
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Patent number: 6960453Abstract: A hybrid type I polyketide synthase gene typically containing a starter module and a plurality of heterologous extender modules is used to synthesize novel polyketides. It is preferably under the control of a type II polypolyketide synthase promoter e.g. act I of S. coelicolor.Type: GrantFiled: July 4, 1997Date of Patent: November 1, 2005Assignee: Biotica Technology LimitedInventors: Peter Francis Leadlay, James Staunton, Jesus Cortes
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Patent number: 6939691Abstract: The use of enzymes that catalyze the production of starter and extender units for polyketides in E. coli and Streptomyces is described; these enzymes include malonyl CoA decarboxylase (MatA), malonyl CoA synthetase (MatB), and a malonate transporter (MatC) as well as proprionyl CoA carboxylase (pcc). The matBC gene from Streptomyces coelicolor, the matABC genes from Rhizobium trifoli, and the pccB and accA2 from Streptomyces coelicolor are useful in specific embodiments of the claimed invention. These enzymes may be used to enhance the yield of polyketides that are natively produced or polyketides that are rationally designed. By using these techniques, the synthesis of a complete polyketide has been achieved in E. coli in the presence of a phosphopantetheinyl transferase, such as sfp from Bacillus subtilis. This achievement permits a host organism with desirable characteristics to be used in the production of such polyketides and to assess the results of gene shuffling.Type: GrantFiled: October 13, 2000Date of Patent: September 6, 2005Assignee: Board of Trustees of the Leland Stanford Junior UniversityInventors: Chaitan Khosla, Blaine Pfeifer
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Patent number: 6927057Abstract: Combinatorial libraries of polyketides can be obtained by suitable manipulation of a host modular polyketide synthase gene cluster such as that which encodes the PKS for erythromycin. The combinatorial library is useful as a source of pharmaceutically active compounds. In addition, novel polyketides and antibiotics are prepared using this method.Type: GrantFiled: May 16, 2001Date of Patent: August 9, 2005Assignees: Kosan Biosciences, The Board of Trustees of the Leland Stanford Junior UniversityInventors: Chaitan Khosla, Gary Ashley, Camilla M. Kao, Robert McDaniel
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Patent number: 6921650Abstract: Recombinant nucleic acids that encode all or a portion of the epothilone polyketide synthase (PKS) are used to express recombinant PKS genes in host cells for the production of epothilones, epothilone derivatives, and polyketides that are useful as cancer chemotherapeutics, fungicides, and immunosuppressants.Type: GrantFiled: November 28, 2000Date of Patent: July 26, 2005Assignee: Kosan Biosciences, Inc.Inventors: Bryan Julien, Leonard Katz, Chaitan Khosla, Li Tang, Rainer Ziermann
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Patent number: 6902913Abstract: Recombinant DNA compounds that encode all or a portion of the narbonolide polyketide synthase are used to express recombinant polyketide synthase genes in host cells for the production of narbonolide, narbonolide derivatives, and polyketides that are useful as antibiotics and as intermediates in the synthesis of compounds with pharmaceutical value.Type: GrantFiled: February 22, 2001Date of Patent: June 7, 2005Assignee: Kosan Biosciences, Inc.Inventors: Melanie C. Betlach, Mary Betlach, Robert McDaniel, Li Tang
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Patent number: 6902925Abstract: The present invention is a process for selective production of beauveriolide I substance or beauveriolide III substance, in which a beauveriolide producing microorganism strain FO-6979 or mutant thereof is cultured in a medium for fungi added with specific amino acid in order to produce beauveriolide I substance or beauveriolide III substance selectively with high yield; beauveriolide I substance or beauveriolide III substance is accumulated selectively in the cultured mass; and beauveriolide I substance or beauveriolide III substance is collected selectively with high yield from said cultured mass.Type: GrantFiled: April 10, 2001Date of Patent: June 7, 2005Assignees: Gakkou Houjin Kitasato Gakuen, Japan Society for the Promotion of ScienceInventors: Satoshi Omura, Hiroshi Tomoda
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Patent number: 6884604Abstract: The present invention provides combinatorial methods for rapidly generating a diverse library of glycorandomized structures, comprising incubating one or more aglycons and a pool of NDP-sugars in the presence of a glycosyltransferase. The glycosyltransferase may be one that is associated with or involved in production of natural secondary metabolites, or one which is putatively associated with or involved in production of natural secondary metabolites. The glycosyltransferase may show significant flexibility with respect to its NDP-sugar donors and/or its aglycons. NDP-sugar donors may be commercially available, or may be produced by utilizing mutant or wild type nucleotidyltransferases significant flexibility with respect to their substrates.Type: GrantFiled: April 1, 2002Date of Patent: April 26, 2005Assignee: Sloan-Kettering Institute for Cancer ResearchInventor: Jon S. Thorson
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Patent number: 6858404Abstract: Nucleic acid molecules are isolated from Sorangium cellulosum that encode polypeptides necessary for the biosynthesis of epothilone. Disclosed are methods for the production of epothilone in recombinant hosts transformed with the genes of the invention. In this manner, epothilone can be produced in quantities large enough to enable their purification and use in pharmaceutical formulations such as those for the treatment of cancer.Type: GrantFiled: November 13, 2001Date of Patent: February 22, 2005Assignee: Novartis AGInventors: Thomas Schupp, James Madison Ligon, Istvan Molnar, Ross Zirkle, Devon Dawn Cyr, Jörn Görlach
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Patent number: 6858411Abstract: Recombinant nucleic acids that encode all or a portion of the epothilone polyketide synthase (PKS) are used to express recombinant PKS genes in host cells for the production of epothilones, epothilone derivatives, and polyketides that are useful as cancer chemotherapeutics, fungicides, and immunosuppressants.Type: GrantFiled: November 28, 2000Date of Patent: February 22, 2005Assignee: Kosan Biosciences, Inc.Inventors: Bryan Julien, Leonard Katz, Chaitan Khosla, Li Tang, Rainer Ziermann
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Patent number: 6846654Abstract: A system for detecting physical or chemical changes in an environment is disclosed. The system comprises means for conducting a chemical reaction; an active biological molecule whose physiological function is noncatalytic which is capable of catalyzing a chemical reaction wherein at least one reactant is converted to one product; and means for sensing and processing information relating to changes in said environment. A method for detecting physical or chemical changes in an environment is also disclosed.Type: GrantFiled: July 9, 1993Date of Patent: January 25, 2005Assignee: IGEN International, Inc.Inventors: Gary F. Blackburn, Charles Durfor, Michael J. Powell, Richard J. Massey
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Patent number: 6838265Abstract: Generic overproduction host cells can be used to produce any polyketide and obviate the need for performing conventional strain improvement.Type: GrantFiled: May 1, 2001Date of Patent: January 4, 2005Assignee: Kosan Biosciences, Inc.Inventors: Daniel Santi, Robert McDaniel, Li Tang, Chaitan Khosla
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Publication number: 20040266701Abstract: A novel family of cyclic polyene natural products isolated from marine actinomycete strain CNQ140 is provided. This novel strain of actinomycetes was obtained from a previously unstudied population of marine actinomycetes that reside in sediments off La Jolla, Calif. Compounds derived from strain CNQ140 have been characterized as having a cyclic polyene-polyol structure; a molecular weight from about 996 to about 1010 in the core ring structure; and at least 58 carbons and at least 14 oxygens. The invention compounds have antitumor and/or anti-microbial activity.Type: ApplicationFiled: June 21, 2004Publication date: December 30, 2004Applicant: The Regents of the University of CaliforniaInventors: William H. Fenical, Paul R. Jensen, Hak Cheol Kwon
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Patent number: 6828126Abstract: The heterologous expression of the oleandomycin polyketide synthase (OlePKS) in Streptomyces lividans, produces 8,8 a-deoxyoleandolide, an aglycone precursor of oleandomycin. The co-expression with deoxyerythronolide B synthase (DEBS) in S. lividans of the P450 monooxygenase OleP produces 8,8 a-dihydroxy-6-deoxyerythonolide B and other derivatives that are precursors to important macrolide antibiotics.Type: GrantFiled: November 16, 2001Date of Patent: December 7, 2004Assignee: Kosan Biosciences, Inc.Inventor: Robert McDaniel
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Publication number: 20040235119Abstract: The invention relates to a method for the production of phospholipids of general formula (I) with the exclusive aid of phospholipase D from a phospholipid mixture and without using organic solvents, wherein phosphatidyl acid is produced in an aqueous, unbuffered phase with phospholipase D and at least one bivalent metal ion in a first step and at least one phospholipid of general formula (I) is produced from the phosphatidyl acid having at least one compound of general formula (II) R3—OH in a second step. Phosphatidylserine, phosphatidylcholine, phosphatidylethanolamine, phosphatidylinositeand/or phosphatidylglycerine are obtained as products. In another embodiment of the invention, the PLD is identical or different in both steps of said method, wherein in the latter case the pH values for the first and second steps of the method are different, said values lying particularly between 6.5 and 8.0, especially between 5.0 and 7.5.Type: ApplicationFiled: February 26, 2004Publication date: November 25, 2004Inventors: Hans-Ulrich Hoppe, Firk Bokenkamp, Sinian Huang
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Publication number: 20040219645Abstract: The complete sequence of the gene cluster for the monensin type I polyketide synthase, from S. cinnamonensis, is provided. Thus variant polyketides containing monensin-derived elements can be genetically engineered. Furthermore there are novel features, e.g. a regulatory protein mon RI, which are of wide utility.Type: ApplicationFiled: May 6, 2002Publication date: November 4, 2004Inventors: Peter Francis Leadley, James Staunton, Mark Yan Oliynyk
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Patent number: 6808903Abstract: Subject of the invention is a process for the preparation of a compound of formula (I) in which R1-R9 represent, independently of each other hydrogen a substituent; m is 0, 1 or 2; n is 0, 1, 2 or 3; and the bonds marked with A, B, C, D, E and F indicate, independently of each other, that two adjacent carbon atoms are connected by a double bond, a single bond, a single bond and an epoxide bridge, or a single bond and a methylene bridge, including, where applicable, an E/Z isomer, a mixture of E/Z isomers, and/or a tautomer thereof, in each case in free form or in salt form, which process comprises I) bringing a compound of formula (II); wherein R1-R7, m, n, A, B, C, D, E and F have the same meanings as given for formula (I) above, into contact with a biocatalyst that is capable of specifically oxidising the alcohol at position 4″ in order to form a compound of formula (III), in which R1, R2, R3, R4, R5, R6, R7, m, n, A, B, C, D, E and F have the meanings given for formula (I); and 2) reacting the compouType: GrantFiled: May 8, 2002Date of Patent: October 26, 2004Assignee: Syngenta Participations AGInventors: Johannes Paul Pachlatko, Thomas Pitterna, Volker Jungmann
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Publication number: 20040166567Abstract: The invention provides strategies, methods, vectors, reagents, and systems for production of synthetic genes, production of libraries of such genes, and manipulation and characterization of the genes and corresponding encoded polypeptides. In one aspect, the synthetic genes can encode polyketide synthase polypeptides and facilitate production of therapeutically or commercially important polyketide compounds.Type: ApplicationFiled: September 26, 2003Publication date: August 26, 2004Inventors: Daniel V. Santi, Ralph C. Reid, Sarah J. Kodumal, Sebastian Jayaraj
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Publication number: 20040146992Abstract: A method for the production of a polyketide by fermentation comprising the steps of growing a culture of a polyketide-producing organism at a pH value conducive to cell growth for a time sufficient to generate the producing culture, lowering the pH of the culture to a value conducive to polyketide product stability, continuing the fermentation until a maximal titer of polyketide is achieved, and optionally extracting the polyketide from the culture.Type: ApplicationFiled: November 12, 2003Publication date: July 29, 2004Inventors: Rika Regentin, Ruchir P. Desai
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Patent number: 6689611Abstract: The present invention is directed to compositions and methods for producing avermectins, and is primarily in the field of animal health. The present invention relates to the identification and characterization of two novel genes, herein referred to as the aveR1 and aveR2 genes, that are involved in regulating avermectin polyketide synthase (PKS) expression and avermectin biosynthesis in Streptomyces avermitilis. The present invention is based on the discovery that inactivation of these genes results in an increase in the amount of avermectin produced by S. avermitilis.Type: GrantFiled: November 16, 2000Date of Patent: February 10, 2004Assignee: Pfizer Inc.Inventors: Kim J. Stutzman-Engwall, Brenda S. Price
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Publication number: 20040023343Abstract: Spinosyn biosynthetic genes, spinosyn producing microorganisms transformed with the biosynthetic genes, methods using the biosynthetic genes to increase production of spinosyn insecticidal macrolides, and methods using the genes or fragments thereof to change the products produced by spinosyn-producing microorganisms.Type: ApplicationFiled: December 23, 2002Publication date: February 5, 2004Inventors: Richard H. Baltz, M. Christine Broughton, Kathryn P. Crawford, Krishnamurthy Madduri, Donald J. Merlo, Patti J. Treadway, Jan R. Turner, Clive Waldron
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Publication number: 20040009560Abstract: The present invention relates to polynucleotide molecules comprising nucleotide sequences encoding an aveC gene product, which polynucleotide molecules can be used to alter the ratio or amount of class 2:1 avermectins produced in fermentation cultures of S. avermitilis. The present invention further relates to vectors, host cells, and mutant strains of S. avermitilis in which the aveC gene has been inactivated, or mutated so as to change the ratio or amount of class 2:1 avermectins produced.Type: ApplicationFiled: June 11, 2003Publication date: January 15, 2004Applicant: Pfizer Inc.Inventors: Kim J. Stutzman-Engwall, Chen Yan, Claes Gustafsson, Anke Krebber, Jeremy Minshull, Sun Ai Raillard
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Publication number: 20040005672Abstract: Recombinant host cells that comprise recombinant DNA expression vectors that drive expression of a product and a precursor for biosynthesis of that product can be used to produce useful products such as polyketides in host cells that do not naturally produce the product or produce the product or precursor at low levels due to the absence of the precursor or the presence of the precursor in rate limiting amounts.Type: ApplicationFiled: February 21, 2003Publication date: January 8, 2004Inventors: Daniel V. Santi, Chaitan Khosla
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Publication number: 20030235892Abstract: Recombinant host cells that comprise recombinant DNA expression vectors that drive expression of a product and a precursor for biosynthesis of that product can be used to produce useful products such as polyketides in host cells that do not naturally produce the product or produce the product at low levels due to the absence of the precursor or the presence of the precursor in rate limiting amounts.Type: ApplicationFiled: June 27, 2003Publication date: December 25, 2003Inventors: Leonard Katz, Peter Revill
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Publication number: 20030232415Abstract: The present invention relates to polynucleotide molecules comprising nucleotide sequences encoding an aveC gene product, which polynucleotide molecules can be used to alter the ratio or amount of class 2:1 avermectins produced in fermentation cultures of S. avermitilis. The present invention further relates to vectors, host cells, and mutant strains of S. avermitilis in which the aveC gene has been inactivated, or mutated so as to change the ratio or amount of class 2:1 avermectins produced.Type: ApplicationFiled: June 11, 2003Publication date: December 18, 2003Applicant: Pfizer Inc.Inventors: Kim J. Stutzman-Engwall, Yan Chen, Claes Gustafsson, Anke Krebber, Jeremy Minshull, Sun Ai Raillard
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Publication number: 20030186899Abstract: Macrolide compounds produced by culturing Saccharopolyspora species LW107129 (NRRL 30141) have insecticidal and acaricidal activity and are useful intermediates for preparing spinosyn analogs.Type: ApplicationFiled: August 13, 2002Publication date: October 2, 2003Inventors: Paul Lewer, Donald R. Hahn, Laura L. Karr, Paul R. Graupner, Jeffrey R. Gilbert, Thomas V. Worden, Raymond C. Yao, Dennis W. Norton
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Publication number: 20030175901Abstract: Host cells comprising recombinant vectors encoding the FK-520 polyketide synthase and FK-520 modification enzymes can be used to produce the FK-520 polyketide. Recombinant DNA constructs comprising one or more FK-520 polyketide synthase domains, modules, open reading frames, and variants thereof can be used to produce recombinant polyketide synthases and a variety of different polyketides with application as pharmaceutical and veterinary products.Type: ApplicationFiled: August 27, 2001Publication date: September 18, 2003Inventors: Christopher Reeves, Daniel Chu, Chaitan Khosla, Daniel Santi, Kai Wu
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Publication number: 20030170725Abstract: Combinatorial libraries of polyketides can be obtained by suitable manipulation of a host modular polyketide synthase gene cluster such as that which encodes the PKS for erythromycin. The combinatorial library is useful as a source of pharmaceutically active compounds. In addition, novel polyketides and antibiotics are prepared using this method.Type: ApplicationFiled: August 6, 2002Publication date: September 11, 2003Inventors: Chaitan Khosla, Camilla M. Kao
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Publication number: 20030166165Abstract: The present invention relates to polynucleotide molecules comprising nucleotide sequences encoding an aveC gene product, which polynucleotide molecules can be used to alter the ratio or amount of class 2:1 avermectins produced in fermentation cultures of S. avermitilis. The present invention further relates to vectors, host cells, and mutant strains of S. avermitilis in which the aveC gene has been inactivated, or mutated so as to change the ratio or amount of class 2:1 avermectins produced.Type: ApplicationFiled: February 10, 2003Publication date: September 4, 2003Inventors: Kim J. Stutzman-Engwall, Anke Krebber, Claes Gustafsson, Jeremy S. Minshull, Sun Ai Raillard, Seran Kim, Yan Chen
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Publication number: 20030162262Abstract: Recombinant DNA compounds that encode all or a portion of the narbonolide polyketide synthase are used to express recombinant polyketide synthase genes in host cells for the production of narbonolide, narbonolide derivatives, and polyketides that are useful as antibiotics and as intermediates in the synthesis of compounds with pharmaceutical value.Type: ApplicationFiled: May 29, 2002Publication date: August 28, 2003Inventors: Gary Ashley, Melanie C. Betlach, Mary Betlach, Robert McDaniel, Li Tang
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Publication number: 20030157652Abstract: The nucleic acid and amino acid sequences of &agr;1, &agr;2, &bgr; and &egr; subunits of acetyl-CoA carboxylase (ACCase) from Streptomyces coelicolor are provided. This subunit structure differs from that of known acyl carboxylases. Materials and methods are provided of increasing ACCase activity and production of secondary metabolites (such as polyketides and especially antibiotics) by causing expression in Streptomyces of such nucleic acid.Type: ApplicationFiled: October 23, 2001Publication date: August 21, 2003Inventors: Hugo Cesar Gramajo, Eduardo Jose Rodriguez
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Publication number: 20030153053Abstract: Ketoreductase (KR) domains of modular polyketide synthase (PKS) enzymes can be inactivated by one or more point mutations in the domain. Replacement or insertion of a KR domain can be used to introduce a cis or trans double bond into a polyketide by appropriate selection or inactivation of the type of KR domain that codes for a particular stereochemical configuration of a hydroxyl moiety. Inactivation of a DH domain can be used to produce a polyketide having a hydroxyl moiety with a desired stereochemical configuration.Type: ApplicationFiled: August 6, 2002Publication date: August 14, 2003Inventor: Ralph Reid
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Publication number: 20030148469Abstract: Combinatorial libraries of polyketides can be obtained by suitable manipulation of a host modular polyketide synthase gene cluster such as that which encodes the PKS for picromycin. The combinatorial library is useful as a source of pharmaceutically active compounds. In addition, novel polyketides and antibiotics are prepared using this method.Type: ApplicationFiled: July 22, 2002Publication date: August 7, 2003Inventors: Gary Ashley, Melanie C. Betlach, Mary Betlach, Robert McDaniel, Li Tang
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Publication number: 20030143666Abstract: The present invention relates to isolated genetic sequences encoding proteins which direct the biosynthesis of the antibiotic everninomicin in Micromonospora carbonacea. The isolated biosynthetic gene cluster serves as a substrate for bioengineering of antibiotic structures.Type: ApplicationFiled: January 26, 2001Publication date: July 31, 2003Inventors: Alfredo Staffa, Emmanuel Zazopoulos, Stephane Mercure, Piotr Peter Nowacki, Chris M. Farnet
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Publication number: 20030138879Abstract: The invention pertains to isolated phosphopantetheinyl transferases, such as the E. coli acyl carrier protein synthase, which transfer a phosphopantetheinyl group onto a substrate. The enzyme can be purified from a natural source, produced recombinantly, or synthetically. Accordingly, the invention provides compositions and kits including phosphopantetheinyl transferases and host cells expressing phosphopantetheinyl transferases. The invention also provides nucleic acids encoding phosphopantetheinyl transferases and vectors comprising such nucleic acids. The invention further provides methods for phosphopantetheinylating a substrate in vitro or in vivo and methods for producing antibiotics in vitro or in vivo.Type: ApplicationFiled: September 30, 2002Publication date: July 24, 2003Applicant: President and Fellows of Harvard CollegeInventors: Ralph H. Lambalot, Amy M. Gehring, Ralph Reid, Christopher T. Walsh