Oxygen Of The Saccharide Radical Is Directly Bonded To A Nonsaccharide Heterocyclic Ring Or A Fused- Or Bridged-ring System Which Contains A Nonsaccharide Heterocyclic Ring (e.g., Coumermycin, Novobiocin, Etc.) Patents (Class 435/75)
  • Patent number: 9511047
    Abstract: The invention relates to a composition comprising: a complex of delphinidin and a sulfoalkyl ether-?-cyclodextrin and/or delphinidin and the salts thereof as a first component; and a tumour-necrosis factor related apoptosis-inducing ligand (TRAIL) as a second component, for use in the treatment of malignant melanoma.
    Type: Grant
    Filed: November 28, 2013
    Date of Patent: December 6, 2016
    Assignee: SAPIOTEC GMBH
    Inventors: Norbert Roewer, Jens Broscheit
  • Patent number: 8911970
    Abstract: Fermentation media containing an isoflavone-depleted soybean meal or isoflavone-depleted soybean meal product and at least one exogenous added ingredient that comprises a substrate for microbial growth are provided. Methods of making a fermentation medium comprising an isoflavone-depleted soybean meal or isoflavone-depleted soybean meal product and methods for obtaining a fermentation product are also provided. The present invention is further directed to fermentation broths obtained by the media and methods. The present invention is also directed to feed additives produced from fermentation broths obtained by the methods.
    Type: Grant
    Filed: March 19, 2013
    Date of Patent: December 16, 2014
    Assignee: Fermalogic, Inc.
    Inventor: J. Mark Weber
  • Publication number: 20140296498
    Abstract: Methods, processes and materials for the production and recovery of Tiacumicins produced by culturing a microorganism belonging to the species Dactylosporangium aurantiacum subspecies hamdenensis having the ability to produce and accumulate one or more Tiacumicin in a nutrient medium comprising a carbon source, a nitrogen source, trace elements such as inorganic salts, and an adsorbent, wherein said nitrogen source comprises fish powder, and wherein said Tiacumicin is produced in a yield greater than about 50 mg/L broth.
    Type: Application
    Filed: May 19, 2014
    Publication date: October 2, 2014
    Applicant: Optimer Pharmaceuticals, Inc.
    Inventors: Youe-Kong Shue, Chi-Jen Frank Du, Ming-His Chiou, Mei-Chiao Wu, Yuan-Ting Chen, Franklin W. Okumu, Jonathan James Duffield
  • Publication number: 20140228554
    Abstract: A method for the synthesis of a fucooligosaccharide glycosides by reacting a fucosyl donor with H-A-R1 or a salt thereof, wherein A and R1 are as defined herein, under the catalysis of an enzyme capable of transferring fucose is provided. The fucooligosaccharid glycoside compounds, or derivatives thereof, their use in the manufacture of human milk oligosaccharides, and a method of manufacture of human milk oligosaccharides, are also provided.
    Type: Application
    Filed: March 19, 2012
    Publication date: August 14, 2014
    Applicant: Glycom A/S
    Inventors: Elise Champion, Gyula Dekany, Markus Hederos, Karoly Agoston
  • Patent number: 8759032
    Abstract: A genetically engineered strain WSJ-IA for producing isovaleryl spiramycin I. Also provided is a method for preparing the strain, including the steps of: (a) constructing a recombinant plasmid including a double gene ist-acyB2; (b) transforming the plasmid into an isovaleryl spiramycin I-producing strain to obtain the strain WSJ-IA. The level of isovaleryl spiramycin I produced by fermentation of the strain WSJ-IA is increased 1.7 times and the fermentation potency thereof increased 4.14 times in comparison with the strain exclusively including a single gene ist.
    Type: Grant
    Filed: July 21, 2011
    Date of Patent: June 24, 2014
    Assignee: Shen Yang Tonglian Group Co., Ltd.
    Inventors: Yiguang Wang, Yang Jiang, Shengwu Yang, Xiaofeng Zhao
  • Patent number: 8741602
    Abstract: Provided is a new method for producing a glucuronide, having excellent productivity and being replaceable with a method using Saccharomyces pombe, and to provide a new means used in this production method. Disclosed are: a transformed Saccharomyces cerevisiae wherein a gene coding for a UDP-glucose dehydrogenase and a gene coding for a UDP-glucose transferase are inserted in a manner such that said genes can be expressed; a transformed Saccharomyces cerevisiae wherein a gene coding for a cytochrome P450 gene is further inserted in a manner such that said gene can be expressed; and a method for producing a glucaronide that includes culturing transformed Saccharomyces cerevisiae in the presence of glucose and a substance to be conjugated, generating the glucuronide of the aforementioned substance to be conjugated.
    Type: Grant
    Filed: February 14, 2011
    Date of Patent: June 3, 2014
    Assignees: Toyama Prefecture, Topu Bio Research Co., Ltd.
    Inventors: Shinichi Ikushiro, Toshiyuki Sakaki, Kaori Yasuda
  • Publication number: 20140147886
    Abstract: A method to increase the production of products of interest in plant material including plant cultures, such as, for example, cell suspension cultures, root cultures, and hairy root cultures is provided. In one embodiment, the method is to contacting the plant material with a precursor or xenobiotic when producing a product of interest from a plant. In another embodiment the plant material is also contacted with a trapping agent. The process may also provide for contacting an elicitor of the product of interest with the plant material. An embodiment provides for contacting an elicitor, precursor and trapping agent with the plant material. The ability to produce novel compounds such as glucosides and glucuronides is provided.
    Type: Application
    Filed: March 5, 2013
    Publication date: May 29, 2014
    Applicant: ARKANSAS STATE UNIVERSITY
    Inventors: Luis Fabricio Medina-Bolivar, Tianhong Yang
  • Publication number: 20140088030
    Abstract: The invention relates to the preparation of phenolics derivatives by enzymatic condensation of phenolics selected among pyrocatechol or its derivatives with the glucose moiety of sucrose. The production of said phenolics derivatives is achieved with a glucosyltransferase (EC 2.4.1.5). These O-?-glucosides of selected phenolics are new, have a solubility in water higher than that of their parent polyphenol and have useful applications in cosmetic and pharmaceutical compositions, such as antioxidative, antiviral, antibacterial, immune-stimulating, antiallergic, antihypertensive, antiischemic, antiarrythmic, antithrombotic, hypocholesterolemic, antilipoperoxidant, hepatoprotective, anti-inflammatory, anticarcinogenic, antimutagenic, antineoplastic, anti-thrombotic and vasodilatory formulations, or in any other field of application.
    Type: Application
    Filed: November 20, 2013
    Publication date: March 27, 2014
    Inventors: DANIEL AURIOL, RENAUD NALIN, PATRICK ROBE, FABRICE LEFEVRE
  • Publication number: 20130344528
    Abstract: The invention relates to a thermo-photo-bioreactor and to a method for the culture and mass micropropagation of Deschampsia antarctica in vitro. The invention comprises a discontinuous immersion reactor for biomass micropropagation, including means for incorporating chemical inducing agents (salt, metals, organic compounds, etc.) and internal luminescence or illumination means (UV radiation and temperature) for supplying said chemical inducers and/or illumination during any growth phase of the vegetable or plant material (multiplication or propagation and/or maintenance). The invention is advantageous in that it can be used to produce large quantities of biomass of the aforementioned Antarctic species, while providing conditions suitable for said plant to produce metabolites that can be used for human health and personal care.
    Type: Application
    Filed: November 9, 2011
    Publication date: December 26, 2013
    Applicant: UNIVERSIDAD SANTIAGO DE CHILE
    Inventors: Gustavo Emilio Zuñiga Navarro, Alvaro Eduardo Sequeida Honorato, Alvaro Gonzalo Castro Oyarzun, Eduardo Andres Tapia Rodriguez, Humberto Godofredo Prieto Encalada, Pablo Andres Zamoro Cantillana
  • Publication number: 20130274213
    Abstract: There is provided a method of glycosylating an aminocoumarin compound comprising conjugating a sugar to the 4?-OH position of the core of the aminocoumarin compound. Also provided is an aminocoumarin compound glycosylated at the 4?-OH position of the core of the aminocoumarin compound. Further aspects of this invention provide this compound for use in therapy, more particularly for use as an antibiotic, or in anticancer treatment.
    Type: Application
    Filed: October 17, 2011
    Publication date: October 17, 2013
    Applicant: UNIVERSITY COLLEGE LONDON
    Inventor: Min Yang
  • Publication number: 20130122541
    Abstract: This invention relates to metabolically engineered microorganism strains, such as bacterial strains, in which there is an increased utilization of malonyl-CoA for production of a chemical product, which includes polyketides and 3-hydroxypropionic acid.
    Type: Application
    Filed: January 27, 2011
    Publication date: May 16, 2013
    Inventors: Michael D. Lynch, Ryan T. Gill, Tanya E.W. Lipscomb
  • Publication number: 20130059341
    Abstract: Provided is a new method for producing a glucuronide, having excellent productivity and being replaceable with a method using Saccharomyces pombe, and to provide a new means used in this production method. Disclosed are: a transformed Saccharomyces cerevisiae wherein a gene coding for a UDP-glucose dehydrogenase and a gene coding for a UDP-glucose transferase are inserted in a manner such that said genes can be expressed; a transformed Saccharomyces cerevisiae wherein a gene coding for a cytochrome P450 gene is further inserted in a manner such that said gene can be expressed; and a method for producing a glucaronide that includes culturing transformed Saccharomyces cerevisiae in the presence of glucose and a substance to be conjugated, generating the glucuronide of the aforementioned substance to be conjugated.
    Type: Application
    Filed: February 14, 2011
    Publication date: March 7, 2013
    Applicant: Toyama Prefecture
    Inventors: Shinichi Ikushiro, Toshiyuki Sakaki, Kaori Yasuda
  • Patent number: 8343951
    Abstract: The object of the present invention is to establish a volatile sulfide production inhibitor that can inhibit the production of volatile sulfides from a composition and to provide a method for inhibiting the production of volatile sulfides from a composition by using the inhibitor. The object are solved by providing a volatile sulfide production inhibitor comprising L-ascorbic acid 2-glucoside as an effective ingredient and a method for inhibiting the production of volatile sulfides from a composition by incorporating the volatile sulfide production inhibitor into the composition.
    Type: Grant
    Filed: February 21, 2007
    Date of Patent: January 1, 2013
    Assignee: Kabushiki Kaisha Hayashibara Seibutsu Kagaku Kenkyujo
    Inventors: Kazuyuki Oku, Mayumi Kurose, Hiroto Chaen, Toshio Miyake
  • Patent number: 8323950
    Abstract: The invention provides a non-naturally occurring microbial organism having an acetyl-CoA pathway and the capability of utilizing syngas or syngas and methanol. In one embodiment, the invention provides a non-naturally occurring microorganism, comprising one or more exogenous proteins conferring to the microorganism a pathway to convert CO, CO2 and/or H2 to acetyl-coenzyme A (acetyl-CoA), methyl tetrahydrofolate (methyl-THF) or other desired products, wherein the microorganism lacks the ability to convert CO or CO2 and H2 to acetyl-CoA or methyl-THF in the absence of the one or more exogenous proteins. For example, the microbial organism can contain at least one exogenous nucleic acid encoding an enzyme or protein in an acetyl-CoA pathway. The microbial organism is capable of utilizing synthesis gases comprising CO, CO2 and/or H2, alone or in combination with methanol, to produce acetyl-CoA.
    Type: Grant
    Filed: May 12, 2011
    Date of Patent: December 4, 2012
    Assignee: Genomatica, Inc.
    Inventors: Mark J. Burk, Christophe H. Schilling, Anthony P. Burgard, John D. Trawick
  • Publication number: 20120034658
    Abstract: Disclosed are methods for obtaining blue anthocyanin pigments and compositions including such pigments. This blue anthocyanin is present at an acidic pH (where anthocyanins are most stable) and may be used as a natural color additive. The methods can include cultivating a plant callus from a plant capable of producing anthocyanin in which the callus is treated with a blue anthocyanin-generating agent at a concentration sufficient to generate callus with blue anthocyanin pigments. The method can also include recovering the blue anthocyanin pigments from the culture. In an example, the blue anthocyanin-generating agent is ammonium molybdate.
    Type: Application
    Filed: May 4, 2009
    Publication date: February 9, 2012
    Applicant: DIANAPLANTSCIENCES, INC.
    Inventors: Sung-Yong Yoon, Helena Mathews
  • Publication number: 20110237533
    Abstract: Compounds represented by formula (I) wherein the symbols are as defined in the description, exhibit a lipase inhibitory activity, and are useful for foods, drinks, and the like.
    Type: Application
    Filed: April 1, 2011
    Publication date: September 29, 2011
    Applicant: AJINOMOTO CO.,INC.
    Inventors: Kenzo NOMURA, Yayoi Kanbayashi
  • Publication number: 20110183930
    Abstract: The invention relates to the preparation of phenolics derivatives by enzymatic condensation of phenolics selected among pyrocatechol or its derivatives with the glucose moiety of sucrose. The production of said phenolics derivatives is achieved with a glucosyltransferase (EC 2.4.1.5). These O-?-glucosides of selected phenolics are new, have a solubility in water higher than that of their parent polyphenol and have useful applications in cosmetic and pharmaceutical compositions, such as antioxidative, antiviral, antibacterial, immune-stimulating, antiallergic, antihypertensive, antiischemic, antiarrythmic, antithrombotic, hypocholesterolemic, antilipoperoxidant, hepatoprotective, anti-inflammatory, anticarcinogenic, antimutagenic, antineoplastic, anti-thrombotic and vasodilatory formulations, or in any other field of application.
    Type: Application
    Filed: March 21, 2011
    Publication date: July 28, 2011
    Applicant: LIBRAGEN
    Inventors: DANIEL AURIOL, Renaud Nalin, Patrick Robe, Fabrice Lefevre
  • Publication number: 20110091726
    Abstract: The present invention aims to provide a particulate composition containing anhydrous crystalline 2-O-?-D-glucosyl-L-ascorbic acid having a significantly, hardly solidifiable property compared to conventional ones in a grade for use in quasi-drugs; a process for producing the same; and uses thereof. The present invention solves the above object by providing a particulate composition containing anhydrous crystalline 2-O-?-D-glucosyl-L-ascorbic in an amount of over 98.0% by weight but less than 99.9% by weight, on a dry solid basis; or a degree of crystallinity of 90% or higher for anhydrous crystalline 2-O-?-D-glucosyl-L-ascorbic acid, when calculated based on a profile of powder X-ray diffraction analysis of the particulate composition, and a dynamic vapor sorption level of 0.01% by weight or lower, when kept at 25° C.
    Type: Application
    Filed: September 3, 2010
    Publication date: April 21, 2011
    Applicant: Kabushiki Kaisha Hayashibara Seibutsu Kagaku Kenkyujo
    Inventors: Takashi Shibuya, Seisuke Izawa, Tomoyuki Nishimoto, Shigeharu Fukuda, Toshio Miyake
  • Publication number: 20110055963
    Abstract: A method of acylating the 3? position of anthocyanin using an enzyme that transfers an aromatic acyl group to a sugar at the 3? position of anthocyanin or a gene encoding the enzyme.
    Type: Application
    Filed: October 28, 2005
    Publication date: March 3, 2011
    Applicant: International Flower Developments Proprietary Limited
    Inventors: Yoshikazu Tanaka, Yukihisa Katsumoto, Masako Mizutani, Yuko Fukui, Junichi Togami
  • Publication number: 20100330009
    Abstract: The present invention relates to derivatives of the 1H-pyrano[4,3-b]benzofuran-1-one structure and their nitrogen analogues which possess powerful antioxidant properties combined with a highly effective UV absorbing functionality in one molecule. These compounds are especially useful in cosmetical and dermatological formulations.
    Type: Application
    Filed: February 29, 2008
    Publication date: December 30, 2010
    Applicant: CIBA CORPORATION
    Inventors: Barbara Wagner, Sebastien Mongiat, Bernd Herzog, Werner Baschong, Andreas Buthe, Reinhold Ohrlein
  • Publication number: 20100323402
    Abstract: The present invention provides a novel UDP-glucuronosyltransferase and a polynucleotide encoding the same (for example, a polynucleotide comprising a polynucleotide consisting of one nucleotide sequence selected from the group consisting of the nucleotide sequence at positions 1 to 1359 in the nucleotide sequence represented by SEQ ID NO: 4, the nucleotide sequence at positions 1 to 1365 in the nucleotide sequence represented by SEQ ID NO: 10, the nucleotide sequence at positions 1 to 1371 in the nucleotide sequence represented by SEQ ID NO: 12, and the nucleotide sequence at positions 1 to 1371 in the nucleotide sequence represented by SEQ ID NO: 22; or a polynucleotide comprising a polynucleotide encoding a protein having one amino acid sequence selected from the group consisting of SEQ ID NOS: 5, 11, 13 and 23), etc. This provides a novel UDP-glucuronosyltransferase with a broad substrate specificity.
    Type: Application
    Filed: September 29, 2008
    Publication date: December 23, 2010
    Applicant: SUNTORY HOLDINGS LIMITED
    Inventors: Eiichiro Ono, Akio Noguchi, Yuko Fukui, Masako Mizutani
  • Publication number: 20100256345
    Abstract: The present invention provides a method for preparing a glycoside of a flavonoid compound, which comprises the step of treating flavonoid and a glycosyl donor with an enzymatic agent having glycosylation activity and being derived from the genus Trichoderma (preferably Trichoderma viride or Trichoderma reesei). Such a flavonoid compound includes a catechin compound or a methylated derivative thereof, and the glycosyl donor includes a carbohydrate containing a maltotriose residue (preferably maltotriose, maltotetraose, maltopentaose, maltohexaose, maltoheptaose, dextrin, ?-cyclodextrin or soluble starch). Glycosides obtained by the present invention have higher water solubility, improved taste, and increased stability. The present invention also provides novel glycosides of catechin compounds, which are obtained by the method of the present invention.
    Type: Application
    Filed: January 18, 2008
    Publication date: October 7, 2010
    Applicant: SUNTORY HOLDINGS LIMITED
    Inventors: Misa Ochiai, Harukazu Fukami, Masahiro Nakao, Akio Noguchi
  • Publication number: 20100143976
    Abstract: A method of increasing production of a methylmalonyl-CoA derivative in a bacterium is disclosed that includes providing a bacterium that produces a methylmalonyl-CoA derivative in which methylmalonyl-CoA mutase function has been inhibited and culturing the bacterium in a medium containing an effective amount of methylmalonyl-CoA or a precursor thereof to increase the production of the methylmalonyl-CoA derivative by the bacterium. The production of the methylmalonyl-CoA derivative is increased when compared to production of the methylmalonyl-CoA derivative by the bacterium when cultured in a medium without the effective amount of methylmalonyl-CoA or a precursor thereof.
    Type: Application
    Filed: November 9, 2009
    Publication date: June 10, 2010
    Applicant: FERMALOGIC, Inc.
    Inventors: J. Mark WEBER, Andrew R. Reeves, Igor A. Brikun, William Henry Cernota
  • Publication number: 20100144658
    Abstract: Seven novel antibiotic substances could be produced by cultivation of a microbial strain which has been isolated from a soil sample and which is designated as Microbispora sp. A 34030 (deposited under an access number FERM BP-10505 in terms of Budapest Treaty). These seven antibiotic substances are named as bispolide A1, bispolide A2, bispolide A3, bispolide B1, bispolide B2a, bispolide B2b and bispolide B3, respectively. These bispolides are each novel compounds which have a chemical structure as collectively represented by the general formula (III) shown below. These bispolides have each a high antibacterial activity against a variety of bacteria, particularly Gram-positive bacteria and their antibiotic-resistant strains, and hence these bispolides each are effective and useful for therapeutically treating bacterial infections of Gram-positive bacteria in human and animals.
    Type: Application
    Filed: May 15, 2007
    Publication date: June 10, 2010
    Applicant: MITSUI CHEMICALS, INC.
    Inventors: Keiichi Numata, Noriyuki Okujo, Siew Eim Khor, Lee Li Tan, Annie George, Szu Ting Ng, Chin Jye Tan, Hironobu Iinuma, Yasuo Fukagawa, Kunimoto Hotta, Seiji Shibahara, Shinichi Kondo, Satoshi Nimura
  • Publication number: 20100121041
    Abstract: Disclosed is a process for producing an oxazoline derivative from a non-protected sugar in a simple manner. Also disclosed is a process for producing a glycoside by utilizing the product of the aforementioned process. A sugar oxazoline derivative is synthesized in one step in an aqueous solution from a sugar having a free hemiacetalic hydroxy group and an amido group by using a haloformamidinium derivative as a dehydrating agent. A glycoside compound is produced by using the oxazoline derivative as a sugar donor and also using a sugar dehydrogenase. The method can be applied to the production of a compound having a long sugar chain, and is therefore useful for the production of a physiologically active oligosaccharide, a carrier for a drug delivery system, a surfactant, a carbohydrate-based drug, a glycopeptide, a glycoprotein, a carbohydrate polymer or the like.
    Type: Application
    Filed: March 7, 2008
    Publication date: May 13, 2010
    Inventors: Shinichiro Shoda, Atsushi Kobayashi, Masato Noguchi, Tomonari Tanaka, Hidetoshi Gyakushi
  • Publication number: 20100113372
    Abstract: Disclosed is a method for the preparation of kaempferol-3-O-rutinoside and a composition of a skin external application comprising kaempferol-3-O-rutinoside as an active ingredient. The method for isolating kaempferol-3-O-rutinoside through hydrolysis uses an enzyme or microbe that removes the sugar selectively from kaempferol-3-O-rutinoside glycosides in a plant extract. Disclosed also is a composition of a skin external application comprising kaempferol-3-O-rutinoside that prevents skin wrinkle.
    Type: Application
    Filed: April 25, 2007
    Publication date: May 6, 2010
    Applicant: Amorepacific Corporation
    Inventors: Jun Seong Park, Hye Yoon Park, Ho Sik Rho, Duck Hee Kim, Ih Seop Chang
  • Publication number: 20100062492
    Abstract: Disclosed are a method for preparing icariside II represented by Formula 1, which inhibits the glycosylation of glycoprotein enzyme tyrosinase by inhibiting the enzymatic activity of alpha-glucosidase, which is an enzyme in the glycosylation of tyrosinase, as well as a skin whitening composition.
    Type: Application
    Filed: September 19, 2007
    Publication date: March 11, 2010
    Applicant: Amorepacific Corporation
    Inventors: Jun Seong Park, Hye Yoon Park, Ho Sik Rho, Soo Mi Ahn, Duck Hee Kim
  • Patent number: 7595175
    Abstract: Biosyntheses of compounds whereof at least portions are polyketides produced by means of polyketide synthase (PKS) enzyme complexes are carried out after specific alterations have been made within the acyltransferase (AT) domains of the PKSs. Particular motifs in or near the substrate binding pocket are disclosed, such that alterations therein affect substrate specificity.
    Type: Grant
    Filed: August 14, 2001
    Date of Patent: September 29, 2009
    Assignee: Biotica Technology Limited
    Inventors: Hrvoje Petkovic, Steven Gary Kendrew, Peter Francis Leadlay
  • Publication number: 20090233876
    Abstract: The invention relates to the preparation of phenolics derivatives by enzymatic condensation of phenolics selected among pyrocatechol or its derivatives with the glucose moiety of sucrose. The production of said phenolics derivatives is achieved with a glucosyltransferase (EC 2.4.1.5). These O-?-glucosides of selected phenolics are new, have a solubility in water higher than that of their parent polyphenol and have useful applications in cosmetic and pharmaceutical compositions, such as antioxidative, antiviral, antibacterial, immune-stimulating, antiallergic, antihypertensive, antiischemic, antiarrhythmic, antithrombotic, hypocholesterolemic, antilipoperoxidant, hepatoprotective, anti-inflammatory, anticarcinogenic antimutagenic, antineoplastic, anti-thrombotic, and vasodilatory formulations, or in any other field of application.
    Type: Application
    Filed: June 13, 2007
    Publication date: September 17, 2009
    Applicant: Libragen
    Inventors: Daniel Auriol, Renaud Nalin, Patrick Robe, Fabrice Lefevre
  • Publication number: 20090191593
    Abstract: The invention provides a non-naturally occurring microbial organism having an acetyl-CoA pathway and the capability of utilizing syngas or syngas and methanol. In one embodiment, the invention provides a non-naturally occurring microorganism, comprising one or more exogenous proteins conferring to the microorganism a pathway to convert CO, CO2 and/or H2 to acetyl-coenzyme A (acetyl-CoA), methyl tetrahydrofolate (methyl-THF) or other desired products, wherein the microorganism lacks the ability to convert CO or CO2 and H2 to acetyl-CoA or methyl-THF in the absence of the one or more exogenous proteins. For example, the microbial organism can contain at least one exogenous nucleic acid encoding an enzyme or protein in an acetyl-CoA pathway. The microbial organism is capable of utilizing synthesis gases comprising CO, CO2 and/or H2, alone or in combination with methanol, to produce acetyl-CoA.
    Type: Application
    Filed: January 22, 2009
    Publication date: July 30, 2009
    Applicant: GENOMATICA, INC.
    Inventors: Mark J. Burk, Christophe H. Schilling, Anthony P. Burgard, John D. Trawick
  • Publication number: 20090143317
    Abstract: The present invention provides an ?-glycosyl isoquercitrin-containing novel composition which has a high in vivo absorbability, and hence exhibits a significant in vivo antioxidative activity. The present invention further provides preparation methods for such a composition. The composition contains a mixture of quercetin glycosides represented by the following formula: wherein Glc represents a glucose residue, and n is 0 or a positive integer of 1 or more, includes at least a quercetin glycoside wherein n is 3, and satisfies the following requirement (a): (a) the total proportion of quercetin glycosides in which n is 3, and in which other n values may be 1 or 2, or 1 and 2, is 50 mol % or more, and the total proportion of quercetin glycosides wherein n is 4 or more is 15 mol % or less, in the composition. The composition can be prepared by treating an enzymatically modified isoquercitrin with ?-amylase.
    Type: Application
    Filed: December 28, 2005
    Publication date: June 4, 2009
    Applicants: Suntory Limited, SAN-EI GEN F.F.I., INC.
    Inventors: Yoshiko Ono, Namino Tomimori, Norifumi Tateishi, Masamitsu Moriwaki, Kazuhiro Emura, Shuji Okuyama
  • Patent number: 7482137
    Abstract: The present invention relates to hybrid glycosylated products, and in particular, to natural products such as polyketides and glycopeptides, and to processes for their preparation. The invention is particularly concerned with recombinant cells in which a cloned microbial glycosyltransferase can be conveniently screened for its ability to generate specific glycosylated derivatives when supplied with polyketide, peptide, or polyketide-peptides as substrates. The invention demonstrates that cloned glycosyltransferases when rapidly screened for their ability to attach a range of activated sugars to a range of exogenously supplied or endogenously generated aglycone templates, show a surprising flexibility towards both aglycone and sugar substrates, and that this process allows the production of glycosylated polyketides in good yield.
    Type: Grant
    Filed: October 12, 2006
    Date of Patent: January 27, 2009
    Assignee: Biotica Technology Limited
    Inventors: Peter Francis Leadlay, James Staunton, Sabine Gaisser
  • Patent number: 7459294
    Abstract: During the production of a product compound by fermentation, the concentration of a precursor compound is maintained within a pre-selected concentration range by having an adsorbent resin in contact with the culture medium. The adsorbent resin reversibly adsorbs precursor compound and, as un-adsorbed precursor compound is converted to product compound, adsorbed precursor compound is released from the resin, thus maintaining the concentration of precursor compound within the pre-selected range.
    Type: Grant
    Filed: August 6, 2004
    Date of Patent: December 2, 2008
    Assignee: Kosan Biosciences Incorporated
    Inventors: Timothy A. Leaf, Ruchir P. Desai, Peter Licari, Elaine Jennifer Woo
  • Publication number: 20080176811
    Abstract: Substituted and unsubstituted flavone or isoflavone glycoside derivatives of the formula [A1-C(?O)O]m—[X—O-Z]—[O—C(?O)-A2]n, wherein [X—O-Z] represents a flavone or isoflavone glycoside structure, particularly a naringin residue, X is a flavone or isoflavone corresponding to formula (IIa) or formula (IIb): wherein the flavone or isoflavone residue is substituted one or more times and/or reduced one or more times; Z represents a mono-, di- or polysaccharide, which is acetally-bound at the benzopyran group to X and ester-substituted by —O—C(?O)-A2; [A1-C(?O)] is an acyl group on the flavone or isoflavone; A1 and A2 independently, represent a polyunsaturated C15-26 alkenyl group containing at least four isolated and/or at least two conjugated double bonds, or an arylaliphatic radical with 1-to-4 methylene groups between the ester group and the aromatic ring; [C(?O)A2] is an acyl group; n is an integer other than 0; m is an integer, including 0; and R1, R2 and R are hydroxyl groups or hydrogen atoms.
    Type: Application
    Filed: March 28, 2008
    Publication date: July 24, 2008
    Inventors: Bernadette Geers, Ralf Otto, Albrecht Weiss, Dirk Petersohn, Klaus Rudolf Schroeder, Kordula Schlotmann
  • Publication number: 20080044861
    Abstract: A method for obtaining improved yields of isoflavones from spent fermentation beer produced by Actinomycetes grown on leguminous plant materials is demonstrated. This method involves conversion of isoflavone biotransformation products to isoflavone aglycones followed by isolation of the isoflavone aglycones. Formation of the biotransformation products had previously limited the production of isoflavone aglycones in these Actinomycete fermentations.
    Type: Application
    Filed: August 15, 2006
    Publication date: February 21, 2008
    Applicant: FERMALOGIC, INC.
    Inventor: J. Mark Weber
  • Patent number: 7300921
    Abstract: Novel polyene polyketides, their pharmaceutically acceptable salts, prodrugs and derivatives have been found to have antibiotic activity. One method for obtaining the compounds is by cultivation of Amycolatopsis orientalis ATCC™ 43491 or a mutant or variant such as the strain IDAC-220604-1. Another method for obtaining the compounds is post-biosynthesis chemical modification of the compounds obtained by cultivation. Novel polynucleotide sequences and encoded proteins for the biosynthesis of the polyene polyketides are also presented.
    Type: Grant
    Filed: September 10, 2004
    Date of Patent: November 27, 2007
    Assignee: Ecopia Biosciences, Inc.
    Inventors: James B. McAlpine, Chris M. Farnet, Emmanuel Zazopoulos, Dan Sorensen
  • Patent number: 7241595
    Abstract: The present invention relates to glycosyl-etoposide prodrugs, a process for the preparation thereof and the use thereof in combination with functionalized tumor-specific enzyme conjugates for treating cancers.
    Type: Grant
    Filed: April 24, 2002
    Date of Patent: July 10, 2007
    Assignee: sanofi-aventis Pharma Deutschland GmbH
    Inventors: Cenek Kolar, Jörg Czech, Klaus Bosslet, Gerhard Seemann, Hans-Harald Sedlacek
  • Patent number: 7211413
    Abstract: Provided are a process for producing purified anthocyanidin glucoside in which a rhamnose end of anthocyanidin rutinoside is cleaved using rhamnosidase to convert the anthocyanidin rutinoside component into anthocyanidin glucoside, the anthocyanidin glucoside component being then purified and isolated; or a crystalline anthocyanidin glucoside salt obtained by further crystallizing the purified anthocyanidin glucoside and a process for producing the same. Also provided are a process for producing purified anthocyanidin rutinoside in which a glucose end of anthocyanidin glucoside is cleaved using ?-glucosidase to degrade and remove the end, the anthocyanidin rutinoside component being then purified and isolated; or a crystalline anthocyanidin rutinoside salt obtained by further crystallizing the purified anthocyanidin rutinoside and a process for producing the same.
    Type: Grant
    Filed: September 11, 2001
    Date of Patent: May 1, 2007
    Assignee: Meiji Seika Kaisha, Ltd.
    Inventors: Hitoshi Matsumoto, Satoshi Hanamura, Masao Hirayama
  • Patent number: 7160700
    Abstract: A nucleic acid encoding a protein which has an amino acid sequence according to SEQ ID NO: 2, 12, or 14, or the amino acid sequence modified by addition or deletion of one or more amino acids and/or substitution by other amino acids, and which has activity to transfer a glycosyl group to the 3?-position of anthocyanins.
    Type: Grant
    Filed: June 1, 2001
    Date of Patent: January 9, 2007
    Assignee: International Flower Developments Proprietary Limited
    Inventors: Masako Mizutani, Keiko Sakakibara, Yoshikazu Tanaka, Takaaki Kusumi, Eiichiro Ono
  • Patent number: 7067290
    Abstract: A method for the production of a polyketide by fermentation comprising the steps of growing a culture of a polyketide-producing organism at a pH value conducive to cell growth for a time sufficient to generate the producing culture, lowering the pH of the culture to a value conducive to polyketide product stability, continuing the fermentation until a maximal titer of polyketide is achieved, and optionally extracting the polyketide from the culture.
    Type: Grant
    Filed: November 12, 2003
    Date of Patent: June 27, 2006
    Assignee: Kosan Biosciences Incorporated
    Inventors: Rika Regentin, Ruchir P. Desai
  • Patent number: 7022825
    Abstract: Facile methods for preparing diketide and triketide thioesters are disclosed. The resulting thioesters may be used as intermediates in the synthesis of desired polyketides, and may contain functional groups which ultimately reside in side chains on the resulting polyketide and thus can be used further to manipulate the polyketide so as form derivatives. The polyketides produced may also be tailored by glycosylation, hydroxylation and the like. New polyketides and their derivatives and tailored forms are thereby produced.
    Type: Grant
    Filed: August 8, 2002
    Date of Patent: April 4, 2006
    Assignee: Kosan Biosciences, Inc.
    Inventors: Gary Ashley, Isaac C. Chan-Kai, Mark A. Burlingame
  • Patent number: 7018808
    Abstract: The rapamycin gene cluster is an example of a gene cluster which includes a gene (rapL) leading to the formation of a precursor compound (pipecolic acid, in this case) which is required for inclusion in the larger product (rapamycin) produced by the enzymes encoded by the cluster. We have produced a mutant strain containing a rapamycin gene cluster in which the rapL gene is disabled. The strain does not naturally produce rapamycin but does so if fed with pipecolic acid. By feeding with alternative carboxylic acids we have produced variants of rapamycins. Tests have shown biological activity.
    Type: Grant
    Filed: December 2, 2002
    Date of Patent: March 28, 2006
    Assignee: Biotica Technology Limited
    Inventors: Peter Francis Leadlay, James Staunton, Lake Ee Khaw
  • Patent number: 7019131
    Abstract: The reactivity of a number of p-methylphenyl thioglycoside (STol) donors which are either fully protected or have one hydroxyl group exposed has been quantitatively determined by HPLC in conjunction with the development of a broadly applicable approach for a facile one-pot synthesis of oligosaccharides. The influence on reactivity of the structural effects of different monosaccharide cores and different protecting groups on each glycoside donor is characterized and quantified. In addition, a correlation between glycosyl donor reactivity and the chemical shift of the anomeric proton by 1 H NMR has been established. A database of thioglycosides as glycosyl donors has been created using this reactivity data. The utility is demonstrated by the easy and rapid one-pot assembly of various linear and branched oligosaccharide structures.
    Type: Grant
    Filed: March 25, 2003
    Date of Patent: March 28, 2006
    Assignee: The Scripps Research Institute
    Inventors: Chi-Huey Wong, Zhiyuan Zhang, Ian Ollmann, Timor Baasov, Xin-Shan Ye
  • Patent number: 6884604
    Abstract: The present invention provides combinatorial methods for rapidly generating a diverse library of glycorandomized structures, comprising incubating one or more aglycons and a pool of NDP-sugars in the presence of a glycosyltransferase. The glycosyltransferase may be one that is associated with or involved in production of natural secondary metabolites, or one which is putatively associated with or involved in production of natural secondary metabolites. The glycosyltransferase may show significant flexibility with respect to its NDP-sugar donors and/or its aglycons. NDP-sugar donors may be commercially available, or may be produced by utilizing mutant or wild type nucleotidyltransferases significant flexibility with respect to their substrates.
    Type: Grant
    Filed: April 1, 2002
    Date of Patent: April 26, 2005
    Assignee: Sloan-Kettering Institute for Cancer Research
    Inventor: Jon S. Thorson
  • Publication number: 20040203015
    Abstract: Materials and methods to produce modified polyketides are disclosed. The biosynthesis, transfer and regulator genes for various sugars to effectuate polyketide modification are disclosed.
    Type: Application
    Filed: June 30, 2003
    Publication date: October 14, 2004
    Inventors: C. Richard Hutchinson, Leonard Katz, Ralph Reid, Zhihao Hu, Hugo Gramajo
  • Publication number: 20040203119
    Abstract: Cell-free systems which effect the production of polyketides employing modular polyketide synthases are described. Libraries of new and/or known polyketides may also be produced in cell-free systems employing aromatic PKS, modular PKS or both.
    Type: Application
    Filed: February 27, 2004
    Publication date: October 14, 2004
    Inventors: Chaitan Khosla, Rembert Pieper, Guanglin Luo, David E. Cane
  • Patent number: 6797508
    Abstract: The invention relates to bioreactor for fermenting solid substrates, comprising a fermentation vessel (2), a device for feeding bioreactive substances and a nozzle arrangement in the fermentation vessel (2). The invention is characterized in that a nozzle arrangement (10, 20) consisting of a plurality of pipes (14, 24) which project into the reaction chamber (49) of the fermentation vessel (2) in parallel and which are provided with nozzles (16, 28) is situated in the fermentation vessel (2). The invention also relates to a method for aerobically fermenting solids. A reaction medium containing these solids is mixed using a compressed gas (48) which is guided into the reaction mixture from above.
    Type: Grant
    Filed: July 31, 2002
    Date of Patent: September 28, 2004
    Assignee: Hofer Bioreact GmbH
    Inventor: Udo Hölker
  • Publication number: 20040161828
    Abstract: This invention provides nucleic acid sequences and characterization of the gene cluster responsible for the biosynthesis of the enediyne C-1027 (produced by Streptomyces globisporus). Methods are provided for the biosynthesis of enediynes, enediyne analogs and other biological molecules.
    Type: Application
    Filed: May 31, 2002
    Publication date: August 19, 2004
    Applicant: The Regents of the University of California
    Inventors: Ben Shen, Wen Liu, Stephen D. Christenson, Scott Standage
  • Publication number: 20040132055
    Abstract: Domains of chalcomycin polyketide synthases and modification enzymes and polynucleotides encoding them are provided. Methods to prepare chalcomycin in pharmaceutically useful quantities are described, as are methods to prepare chalcomycin analogs and other polyketides using the polynucleotides encoding chalcomycin polyketide synthase domains or modifying enzymes.
    Type: Application
    Filed: August 21, 2003
    Publication date: July 8, 2004
    Inventors: Leonard Katz, Ralph C. Reid, Zhihao Hu, Andreas Schirmer, Shannon L. Ward, Christopher Reeves
  • Publication number: 20040115762
    Abstract: The invention provides a nucleic acid molecule comprising: (a) a nucleotide sequence as shown in SEQ ID No. 35; or (b) a nucleotide sequence which is the complement of SEQ ID No. 35; or (c) a nucleotide sequence which is degenerate with SEQ ID No. 35; or (d) a nucleotide sequence hybridising under conditions of high stringency to SEQ ID No. 35, to the complement of SEQ ID No. 35, or to a hybridisation probe derived from SEQ ID No. 35 or the complement thereof; or (e) a nucleotide sequence having at least 80% sequence identity with SEQ ID No. 35; or (f) a nucleotide sequence having at least 65% sequence identity with SEQ ID No. 35 wherein said sequence preferably encodes or is complementary to a sequence encoding a nystatin PKS enzyme or a part thereof.
    Type: Application
    Filed: February 7, 2003
    Publication date: June 17, 2004
    Inventors: Sergey Borisovich Zotchev, Olga Nikolayivna Sekurova, Espen Fjaervik, Trygve Brautaset, Arne Reidar Strom, Svein Valla, Trond Erling Ellingsen, Havard Sletta, Ole-Martin Gulliksen