Having A Fused Ring Containing A Six-membered Ring Having Two N-atoms In The Same Ring (e.g., Purine Nucleosides, Etc.) Patents (Class 435/88)
  • Patent number: 10724058
    Abstract: A process of producing methacrylic acid and/or derivatives thereof including the following steps: (a) biologically converting isobutyryl-CoA into methacrylyl-CoA by the action of an oxidase; and (b) converting methacrylyl-CoA into methacrylic acid and/or derivatives thereof. The invention also extends to microorganisms adapted to conduct the steps of the process.
    Type: Grant
    Filed: November 20, 2017
    Date of Patent: July 28, 2020
    Assignee: LUCITE INTERNATIONAL UK LIMITED
    Inventors: Graham Ronald Eastham, Gill Stephens, Andrew Yiakoumetti
  • Patent number: 10704063
    Abstract: A process of producing methacrylic acid and/or derivatives thereof including the following steps: (a) biologically converting isobutyryl-CoA into methacrylyl-CoA by the action of an oxidase; and (b) converting methacrylyl-CoA into methacrylic acid and/or derivatives thereof. The invention also extends to microorganisms adapted to conduct the steps of the process.
    Type: Grant
    Filed: May 18, 2016
    Date of Patent: July 7, 2020
    Assignee: LUCITE INTERNATIONAL UK LIMITED
    Inventors: Graham Ronald Eastham, Gill Stephens, Andrew Yiakoumetti
  • Patent number: 10570426
    Abstract: Provided is a method for producing a methacrylic acid ester using a biocatalyst, said method comprising a step of reacting an alcohol or a phenol with methacrylyl-CoA in the presence of an alcohol acyltransferase originated from a plant selected from the group consisting of a plant belonging to the genus Osmanthus, a plant belonging to the genus Vitis, a plant belonging to the genus Citrus, a plant belonging to the genus Durio, a plant belonging to the genus Magnolia and a plant belonging to the genus Chamaemelum to thereby synthesize the methacrylic acid ester.
    Type: Grant
    Filed: March 5, 2015
    Date of Patent: February 25, 2020
    Assignee: Mitsubishi Chemical Corporation
    Inventors: Yasuhisa Asano, Eiji Sato, Fujio Yu, Wataru Mizunashi
  • Patent number: 10378029
    Abstract: A method produces a chemical product by continuous fermentation including filtering a culture liquid of a microorganism(s) through a separation membrane, retaining unfiltered liquid in, or refluxing unfiltered liquid to, the culture liquid, adding a fermentation feedstock to the culture liquid, and recovering a product in the filtrate, wherein the microorganism(s) is/are a microorganism(s) that undergo(es) catabolite repression, and the fermentation feedstock comprises hexose and pentose.
    Type: Grant
    Filed: January 11, 2013
    Date of Patent: August 13, 2019
    Assignee: Toray Industries, Inc.
    Inventors: Koji Kobayashi, Shiomi Watanabe, Kyohei Isobe, Kenji Sawai, Kyungsu Na, Shingo Hiramatsu, Katsushige Yamada
  • Patent number: 9745337
    Abstract: The invention relates to the development of drugs intended for the prophylaxis and/or treatment of viral diseases caused, in particular, by herpes viruses. What are proposed are complex compounds of germanium having the general structural formula: Gex[AD][CA]y[AA]2 ??(1), where AD is a derivative of a nitrogenous base of the purine series that has antiviral activity and can be selected from guanine derivatives, such as acyclovir, valacyclovir, gancyclovir and pencyclovir, or from adenine derivatives, such as vidarabine; CA is a hydroxycarboxylic acid which can be selected from acids such as (but not limited to) citric acid, lactic acid and malic acid; AA is an amino acid which can be selected from various a-amino acids, such as arginine, gylcine, lysine and threonine, and where x=1-2, y=2-4 and z=0-2. Complex compounds of germanium have a high level of antiviral and immune-stimulating activity and are readily soluble in water.
    Type: Grant
    Filed: November 1, 2012
    Date of Patent: August 29, 2017
    Assignee: OBSCHESNO S OGRANICHENNOI OTVETSTVENNOSTYU “WDS FARMA”
    Inventors: Alexandr Dmitrievich Isaev, Igor Valerievich Ambrosov, Tamaz Omarovich Manasherov, Svetlana Konstantinovna Matelo
  • Publication number: 20150140606
    Abstract: A method of producing a sugar liquid from cellulose-containing biomass includes (1) to (4): (1) subjecting a cellulose-containing biomass to a dilute sulfuric acid treatment and thereafter separating the treated cellulose-containing biomass into a dilute sulfuric acid-treated liquid and a cellulose-containing solid content; (2) adding a cellulase to the cellulose-containing solid content to hydrolyze the cellulose and thereafter obtaining a sugar liquid; (3) filtering the dilute sulfuric acid-treated liquid through a nanofiltration membrane at pH 2.5 or lower to thereby separate a sugar concentrated liquid as a retentate and at the same time recover a sulfuric acid aqueous solution as a permeate; and (4) reusing the whole amount or a part of the sulfuric acid aqueous solution obtained in (3) in the dilute sulfuric acid treatment in (1).
    Type: Application
    Filed: June 11, 2013
    Publication date: May 21, 2015
    Inventors: Atsushi Minamino, Hiroyuki Kurihara, Katsushige Yamada
  • Publication number: 20150057243
    Abstract: Methods and compositions disclosed herein relate to detecting, analyzing, isolating and inhibiting methyltransferases, methyltransferase substrates, S-adenosyl-methionine-binding proteins and RNA, including for the treatment of disease.
    Type: Application
    Filed: April 2, 2013
    Publication date: February 26, 2015
    Applicant: Northern University
    Inventors: Zhaohui Sunny Zhou, Wanlu Qu, Tianyi Bai
  • Publication number: 20150050694
    Abstract: A method of sterilizing a separation membrane module using water vapor includes: a liquid supplying step of supplying a liquid having a boiling point of 80° C. or higher at atmospheric pressure to a secondary side of the separation membrane module such that a filling ratio of the liquid in a space surrounded by a filtration portion of a separation membrane is 70% or more, the filtration portion being used for filtration; a liquid sealing step of isolating the secondary side of the separation membrane module such that the filling ratio of the liquid supplied to the secondary side in the liquid supplying step is 70% or more; and a sterilization step of sterilizing the separation membrane module by supplying water vapor to a primary side of the separation membrane module while the secondary side of the separation membrane module is isolated.
    Type: Application
    Filed: March 1, 2013
    Publication date: February 19, 2015
    Applicant: TORAY INDUSTRIES, INC.
    Inventors: Norihiro Takeuchi, Atsushi Kobayashi
  • Publication number: 20140363847
    Abstract: An acetyl-CoA-producing microorganism, which is capable of efficiently synthesizing acetyl-CoA using carbon dioxide, and a substance production method using the same are provided. An acetyl-CoA-producing microorganism including an acetyl-CoA production cycle obtained by imparting at least one type of enzymatic activity selected from the group consisting of malate thiokinase, malyl-CoA lyase, glyoxylate carboligase, 2-hydroxy-3-oxopropionate reductase, and hydroxypyruvate reductase, to a microorganism.
    Type: Application
    Filed: July 27, 2012
    Publication date: December 11, 2014
    Inventors: Ryota Fujii, Tomokazu Shirai, Tadashi Araki, Koh Amano, Yoshiko Matsumoto, Toshihiro Tateno, Nozomi Takebayashi, Takashi Morishige, Hitoshi Takahashi, Mitsufumi Wada, Hiroshi Shimizu, Chikara Furusawa, Takashi Hirasawa, Tomonori Hidesaki, Ayako Endo, Dominik Lukas Jürgen-Lohmann, Anjali Madhavan
  • Patent number: 8859236
    Abstract: The present invention relates to a microorganism with improved production of 5?-xanthosine monophosphate and 5?-guanine monophosphate, and more specifically, to a Corynebacterium sp. microorganism having increased proline dehydrogenase activity compared with an intrinsic activity thereof, a method for producing 5?-xanthosine monophosphate or 5?-guanine monophosphate from the culture medium obtained by culturing the transformed microorganism, and a use of the microorganism for production of 5?-xanthosine monophosphate or 5?-guanine monophosphate.
    Type: Grant
    Filed: March 17, 2011
    Date of Patent: October 14, 2014
    Assignee: CJ Cheiljedang Corporation
    Inventors: Jin Man Cho, Jin Nam Lee, Hye Won Kim, Ji Hye Lee, Nan Young Yoon, Kwang Woo Lee, Yoon Seok Oh, Jang Hee Park
  • Publication number: 20140302560
    Abstract: A method of creating an extract of myceliated agricultural product for human consumption includes providing an agricultural substrate such as rice, where the agricultural substrate has been inoculated by liquid media comprising an aliquot of culture derived from liquid-state fermentation. The culture being selected from the group consisting of Basidiomycota and Ascomycota fungi. Next, the step of enabling mycelium growth on the substrate by controlling temperature, humidity and sterility of the environment. After mycelium growth on the substrate reaches a desired stage, then the step of boiling the substrate in water and separating the water-substrate mixture into aqueous component and non-aqueous components creates an extraction.
    Type: Application
    Filed: March 15, 2013
    Publication date: October 9, 2014
    Inventor: Brooks John Kelly
  • Publication number: 20140287461
    Abstract: A method of producing a sugar liquid using a cellulose-containing biomass as a raw material includes (a) hydrolyzing a cellulose-containing biomass to produce an aqueous sugar solution and (b) filtering the obtained aqueous sugar solution through a reverse osmosis membrane to collect a purified sugar liquid from a feed side, while removing fermentation-inhibiting substances from a permeate side.
    Type: Application
    Filed: May 28, 2014
    Publication date: September 25, 2014
    Applicant: Toray Industries, Inc.
    Inventors: Hiroyuki Kurihara, Atsushi Minamino, Masateru Ito, Hideki Sawai, Masayuki Hanakawa, Shin-ichi Minegishi, Katsushige Yamada
  • Publication number: 20140178937
    Abstract: A method of producing a sugar liquid with a cellulose-containing biomass as a raw material includes (1) hydrolyzing a cellulose-containing biomass to produce an aqueous sugar solution, and (2) filtering the aqueous sugar solution obtain in (1) through an ultrafiltration membrane having a molecular weight cutoff of 600 to 2,000 to remove a fermentation inhibitor(s) into the permeate side and collect a sugar liquid from the feed side.
    Type: Application
    Filed: July 27, 2012
    Publication date: June 26, 2014
    Applicant: Toray Industries, Inc.
    Inventors: Atsushi Minamino, Hiroyuki Kurihara, Katsushige Yamada
  • Patent number: 8759034
    Abstract: A recombinant expression vector comprising: a) the sequence encoding a purine nucleoside phosphorylase (PNPase, E. C. 2.4.2.1), b) the sequence encoding a uridine phosphorylase (UPase, E. C. 2.4.2.3), c) or both; each of the sequences operably linked to one or more control sequences that direct the production of said phosphorylases in a suitable expression host; said sequences originating from the Archaea Thermoprotei class, characterized in that the PNPase is from Sulfolobus solfataricus (SEQ ID NO. 7) and the UPase is from Aeropyrum pernix (SEQ ID NO. 8). In addition, the present invention relates to A transglycosylation method between a sugar-donating nucleoside and an acceptor base in the presence of phosphate ions, characterized in that said method comprises the use of a uridine phosphorylase (UPase) of Aeropyrum pernix (NC_000854.2), a purine nucleoside phosphorylase (PNpase) of Sulfolobus solfataricus (NC_002754.1), or a combination thereof.
    Type: Grant
    Filed: July 22, 2013
    Date of Patent: June 24, 2014
    Assignee: Plasmia Biotech, S.L.
    Inventors: Rafael Montilla Arevalo, Victor Manuel DeronceléThomas, Cristina López Gómez, Marta Pascual Gilabert, Carlos Estevez Company, Josep Castells Boliart
  • Patent number: 8741599
    Abstract: A process for the fermentative production of at least one organic compound having at least 3 C atoms or having at least 2 C atoms and at least one 1 N atom, comprising the following steps: a1) milling a starch feedstock, thus obtaining a millbase which comprises at least part of the nonstarchy solid constituents of the starch feedstock; a2) suspending the millbase in an aqueous liquid and hydrolysis of the starch portion in the millbase by enzymatic liquefaction and, if appropriate, subsequent saccharification, whereby a first liquid (1) which comprises mono- or oligosaccharides is obtained; and b) addition of the liquid (1) which comprises mono- or oligosaccharides together with metabolizable mono-, di- or oligosaccharides or together with a composition which comprises metabolizable mono-, di- or oligosaccharide in a concentration of at least 50% by weight and which is essentially free from solids which are insoluble in water to a fermentation medium comprising a microorganism which is capable of overproduc
    Type: Grant
    Filed: November 27, 2006
    Date of Patent: June 3, 2014
    Assignee: BASF SE
    Inventors: Markus Pompejus, Stephan Freyer, Markus Lohscheidt, Oskar Zelder, Matthias Boy
  • Patent number: 8669078
    Abstract: Disclosed herein are ammonia-specific 5?-XMP aminase mutants and a method for preparing the same. A mutation is introduced into the active site of glutamine-dependent catalysis in 5?-XMP aminase. The resulting 5?-XMP aminase mutant is devoid of the glutamine-dependent activity and specifically reacts with external ammonia in converting 5?-XMP into 5?-GMP. Thus, the ammonia-specific 5?-XMP aminase mutant is stabler within cells compared to the wild type, and can be useful in the industrial conversion of 5?-XMP into 5?-GMP.
    Type: Grant
    Filed: December 14, 2006
    Date of Patent: March 11, 2014
    Assignee: CJ Cheiljedang Corporation
    Inventors: Jae-Gu Pan, Heung-Chae Jung, Eui-Joong Kim, Han-Seung Lee, Young Hoon Park, Hyoung Suk Kim, Jong-Kwon Han, Jin Nam Lee, Ki-Hoon Oh, Jeong Hwan Kim, Yoon-Suk Oh, Jae Ick Sim, Kuk-Ki Hong, Kyung Oh Choi, Hyun Soo Kim, Min-Ji Baek, Tae Sun Kang
  • Publication number: 20130273608
    Abstract: A method of producing a compound originating from a polysaccharide-based biomass includes at least one of a saccharification step that produces a sugar solution containing a monosaccharide and/or an oligosaccharide from a product obtainable by hydrolyzing the polysaccharide-based biomass; a fermentation step that ferments the sugar solution containing the monosaccharide and/or oligosaccharide originating from the polysaccharide-based biomass; and a treatment that removes a fermentation inhibitor with the use of a separation membrane having a glucose removal rate and an isopropyl alcohol removal rate which simultaneously satisfy the following relationships (I) and (II) when a 500 ppm aqueous glucose solution at pH 6.5 at 25° C. and a 500 ppm aqueous isopropyl alcohol solution at pH 6.5 at 25° C. are respectively permeated through the membrane at an operation pressure of 0.
    Type: Application
    Filed: June 12, 2013
    Publication date: October 17, 2013
    Inventors: Masayuki Hanakawa, Shinichi Minegishi, Hiroyuki Kurihara
  • Patent number: 8551736
    Abstract: A microorganism of the genus Corynebacterium having the ability to produce inosine in which the inosine catabolic pathway is blocked and that has a leaky adenine auxotrophic phenotype and further has a leaky guanine auxotrophic phenotype and a method of producing inosine, the method including culturing the microorganism of the genus Corynebacterium are disclosed.
    Type: Grant
    Filed: January 2, 2009
    Date of Patent: October 8, 2013
    Assignee: CJ Cheiljedang Corp.
    Inventors: Chul Ha Kim, Jong Soo Choi, Jeong Hwan Kim, Hyoung Seok Kim, Jung Gun Kwon, Tae Min Ahn, Soo Youn Hwang, Jae Ick Sim, Min Ji Baek, Na Ra Kwon, Hye Jin Choi
  • Publication number: 20130095529
    Abstract: The present invention relates to a microorganism with improved production of 5?-xanthosine monophosphate and 5?-guanine monophosphate, and more specifically, to a Corynebacterium sp. microorganism having increased proline dehydrogenase activity compared with an intrinsic activity thereof, a method for producing 5?-xanthosine monophosphate or 5?-guanine monophosphate from the culture medium obtained by culturing the transformed microorganism, and a use of the microorganism for production of 5?-xanthosine monophosphate or 5?-guanine monophosphate.
    Type: Application
    Filed: March 17, 2011
    Publication date: April 18, 2013
    Inventors: Jin Man Cho, Jin Nam Lee, Hye Won Kim, Ji Hye Lee, Nan Young Yoon, Kwang Woo Lee, Yoon Seok Oh, Jang Hee Park
  • Patent number: 8420354
    Abstract: This application provides a process for preparing enantiomerically pure ?-D-dioxolane nucleosides. In particular, a new synthesis of (?)-DAPD, suitable for large scale development, is described. In one embodiment the invention provides a process for preparing a substantially pure ?-D- or ?-L-1,3-dioxolane nucleosides comprising a) preparing or obtaining an esterified 2,2-dialkoxy ethanol; b) cyclizing the esterified 2,2-dialkoxy ethanol with glycolic acid to obtain a 1,3-dioxolane lactone; c) resolving the 1,3-dioxolane lactone to obtain a substantially pure D- or L-lactone; d) selectively reducing and activating the D- or L-chiral lactone to obtain a substantially pure D- or L-1,3-dioxolane; e) coupling the D- or L-1,3-dioxolane to an activated and/or protected purine or pyrimidine base; and f) optionally purifying the nucleoside to obtain a substantially pure protected ?-D- or ?-L-1,3-dioxolane nucleoside.
    Type: Grant
    Filed: August 27, 2010
    Date of Patent: April 16, 2013
    Assignee: Emory University
    Inventors: Marcos Sznaidman, George R. Painter, Merrick R. Almond, Darryl G. Cleary, Amir Pesyan
  • Publication number: 20130084600
    Abstract: This invention is metabolically engineer bacterial strains that provide increased intracellular NADPH availability for the purpose of increasing the yield and productivity of NADPH-dependent compounds. In the invention, native NAD-dependent GAPDH is replaced with NADP-dependent GAPDH plus overexpressed NADK. Uses for the bacteria are also provided.
    Type: Application
    Filed: November 19, 2012
    Publication date: April 4, 2013
    Applicant: WILLIAM MARSH RICE UNIVERSITY
    Inventor: WILLIAM MARSH RICE UNIVERSITY
  • Publication number: 20130065279
    Abstract: The invention provides a non-naturally occurring microbial organism having a methacrylic acid, methacrylate ester, 3-hydroxyisobutyrate and/or 2-hydroxyisobutyrate pathway. The microbial organism contains at least one exogenous nucleic acid encoding an enzyme in a methacrylic acid pathway. The invention additionally provides a method for producing methacrylic acid, methacrylate ester, 3-hydroxyisobutyrate and/or 2-hydroxyisobutyrate. The method can include culturing methacrylic acid, methacrylate ester, 3-hydroxyisobutyrate and/or 2-hydroxyisobutyrate producing microbial organism, where the microbial organism expresses at least one exogenous nucleic acid encoding a methacrylic acid pathway enzyme in a sufficient amount to produce methacrylic acid, methacrylate ester, 3-hydroxyisobutyrate and/or 2-hydroxyisobutyrate, under conditions and for a sufficient period of time to produce methacrylic acid, methacrylate ester, 3-hydroxyisobutyrate and/or 2-hydroxyisobutyrate.
    Type: Application
    Filed: March 30, 2012
    Publication date: March 14, 2013
    Applicant: Genomatica, Inc.
    Inventors: Mark J. BURK, Anthony P. Burgard, Robin E. Osterhout, Jun Sun, Priti Pharkya
  • Publication number: 20130059332
    Abstract: The present invention relates to a process for the production of an aqueous glucose solution from maize or maize kernels. The invention also relates to a glucose solution obtainable by this process, and to its use for the production of organic compounds. The process according to the invention comprises: a) fractionating dry milling of maize kernels, where the maize kernels are separated into a maize-starch-comprising endosperm fraction and a high-oil germ fraction and, if appropriate, a bran fraction; b) enzymatic liquefaction and saccharification of the maize starch in an aqueous suspension of the endosperm fraction, which gives an aqueous glucose solution comprising maize gluten; and c) depletion of the maize gluten and, if appropriate, any bran present from the aqueous glucose solution.
    Type: Application
    Filed: September 14, 2012
    Publication date: March 7, 2013
    Applicant: BASF SE
    Inventors: Matthias Boy, Jong-Kyu Choi, Jin Won Chung, Markus Lohscheidt, Jong In Choi, Jae Yeol Seo, Jörg Braun, Mo Se Kim, Sung Hyun Kim, Arno Kochner
  • Publication number: 20120264175
    Abstract: The present invention relates to a recombinant expression vector comprising: a) the sequence encoding a purine nucleoside phosphorylase (PNPase, E. C. 2.4.2.1), b) the sequence encoding a uridine phosphorylase (UPase, E. C. 2.4.2.3), c) or both; each of the sequences operably linked to one or more control sequences that direct the production of said phosphorylases in a suitable expression host; said sequences originating from the Archaea Thermoprotei class, characterized in that the PNPase is from Sulfolobus solfataricus (SEQ ID NO. 7) and the UPase is from Aeropyrum pernix (SEQ ID NO. 8). In addition, the present invention relates to A transglycosylation method between a sugar-donating nucleoside and an acceptor base in the presence of phosphate ions, characterised in that said method comprises the use of a uridine phosphorylase (UPase) of Aeropyrum pernix (NC_000854.2), a purine nucleoside phosphorylase (PN-Pase) of Sulfolobus solfataricus (NC_002754.1), or a combination thereof.
    Type: Application
    Filed: December 22, 2010
    Publication date: October 18, 2012
    Inventors: Rafael Montilla Arevalo, Víctor Manuel Deroncelé, Cristina López Gómez, Marta Pascual Gilabert, Carlos Estévez Company, Josep Castells Boliart
  • Patent number: 8236531
    Abstract: A purine-derived substance is produced by culturing a bacterium belonging to the genus Bacillus which has an ability to produce a purine-derived substance and has been modified so that enzymatic activity of transaldolase is decreased in a medium to cause accumulation of a purine-derived substance in the medium or cells, and collecting the purine-derived substance from the medium or cells.
    Type: Grant
    Filed: October 21, 2008
    Date of Patent: August 7, 2012
    Assignee: Ajinomoto Co., Inc.
    Inventors: Takayuki Asahara, Kiyoshi Matsuno, Yukiko Mori
  • Patent number: 8187840
    Abstract: The present invention relates to a microorganism producing inosine, which is one of purine nucleoside, an important material for 5?-inosinic acid synthesis, and method for producing inosine using the same. More particularly, the present invention relates to a recombinant microorganism of Corynebacterium genus producing inosine at high concentration by inactivating the gene encoding nucleoside hydrolase II and by enhancing the expression of the gene encoding 5?-nucleotidase, which still retains the characteristics of Corynebacterium ammoniagenes CJIP2401 (KCCM-10610).
    Type: Grant
    Filed: September 9, 2011
    Date of Patent: May 29, 2012
    Assignee: CJ Cheiljedang Corporation
    Inventors: Young-hoon Park, Kwang-myung Cho, Hee-jong Lee, Jin-nam Lee
  • Publication number: 20120129222
    Abstract: Durability of formate dehydrogenase is improved with the use of formate dehydrogenase exhibiting high specific activity that is unpredictable from conventional findings. A specific amino acid substitution is introduced into Gibberella zeae-derived formate dehydrogenase. Mutant formate dehydrogenase exhibits durability that is extremely superior to that of wild-type formate dehydrogenase. Thus, the productivity of NADH that is produced using the mutant formate dehydrogenase can be improved.
    Type: Application
    Filed: August 3, 2009
    Publication date: May 24, 2012
    Applicant: TOYOTA JIDOSHA KABUSHIKI KAISHA
    Inventor: Takahiro Kusumegi
  • Patent number: 8178322
    Abstract: A method is described for producing an L-amino acid or a nucleic acid by culturing a microorganism having an ability to produce the L-amino acid or nucleic acid in a liquid medium in a fermentation tank containing a stirring impeller, and optionally adding seed crystals to the medium as required to produce and accumulate crystals of the L-amino acid or nucleic acid in the medium, and collecting crystals of the L-amino acid or nucleic acid from the culture. The power density of the stirring impeller is controlled to be 2.4 kW/m3 or lower after either precipitation of the crystals or addition of the seed crystals.
    Type: Grant
    Filed: August 14, 2009
    Date of Patent: May 15, 2012
    Assignee: Ajinomoto Co., Inc.
    Inventors: Masayuki Araki, Yusuke Takahashi, Akihiro Watanabe, Fumito Ohnishi, Takahiro Asano, Kazuya Kondo, Wataru Hibino, Shintaro Iwatani, Satoshi Okutani
  • Patent number: 8168422
    Abstract: The present invention relates to a microorganism producing inosine, which is one of purine nucleoside, an important material for 5?-inosinic acid synthesis, and method for producing inosine using the same. More particularly, the present invention relates to a recombinant microorganism of Corynebacterium genus producing inosine at high concentration by inactivating the gene encoding nucleoside hydrolase II and by enhancing the expression of the gene encoding 5?-nucleotidase, which still retains the characteristics of Corynebacterium ammoniagenes CJIP2401 (KCCM-10610).
    Type: Grant
    Filed: January 15, 2008
    Date of Patent: May 1, 2012
    Assignee: CJ Cheiljedang Corporation
    Inventors: Young-hoon Park, Kwang-myung Cho, Hee-jong Lee, Jin-nam Lee
  • Publication number: 20120040923
    Abstract: Stable compositions of defined non-racemic diastereomeric ratios of S-adenosyl-L-methionine, methods for their synthesis and methods for their uses are described. The compositions according to the invention are very stable and are valuable for use as active constituents in pharmaceutical compositions.
    Type: Application
    Filed: October 27, 2011
    Publication date: February 16, 2012
    Inventor: Rolland F. Hebert
  • Publication number: 20110318783
    Abstract: The present invention relates to a microorganism producing inosine, which is one of purine nucleoside, an important material for 5?-inosinic acid synthesis, and method for producing inosine using the same. More particularly, the present invention relates to a recombinant microorganism of Corynebacterium genus producing inosine at high concentration by inactivating the gene encoding nucleoside hydrolase II and by enhancing the expression of the gene encoding 5?-nucleotidase, which still retains the characteristics of Corynebacterium ammoniagenes CJIP2401 (KCCM-10610).
    Type: Application
    Filed: September 9, 2011
    Publication date: December 29, 2011
    Applicant: CJ CHEILJEDANG CORPORATION
    Inventors: Young-hoon Park, Kwang-myung Cho, Hee-jong Lee, Jin-nam Lee
  • Publication number: 20110229937
    Abstract: Isolated nucleic acid molecules, designated MR nucleic acid molecules, which encode novel MR proteins from Corynebacterium glutamicum are described which are involved in biosynthesis of a fine chemical, e.g., methionine biosynthesis. The invention also provides antisense nucleic acid molecules, recombinant expression vectors containing MR nucleic acid molecules, and host cells into which the expression vectors have been introduced. The invention still further provides methods of producing methionine from microorganisms, e.g., C. glutamicum, which involve culturing recombinant microorganisms which overexpress or underexpress at least one MR molecule of the invention under conditions such that methionine is produced. Also featured are methods of producing a fine chemical, e.g., methionine, which involve culturing recombinant microorganisms having selected MR genes deleted or mutated under conditions such that the fine chemical, e.g., methionine, is produced.
    Type: Application
    Filed: January 31, 2011
    Publication date: September 22, 2011
    Applicant: Evonik Degussa GMBH
    Inventors: Markus Pompejus, Burkhard Kröger, Hartwig Schröder, Oskar Zelder, Gregor Haberhauer, Stefan Haefner, Corrinna Klopprogge
  • Publication number: 20110223637
    Abstract: The invention provides a non-naturally occurring microbial organism having an acetyl-CoA pathway and the capability of utilizing syngas or syngas and methanol. In one embodiment, the invention provides a non-naturally occurring microorganism, comprising one or more exogenous proteins conferring to the microorganism a pathway to convert CO, CO2 and/or H2 to acetyl-coenzyme A (acetyl-CoA), methyl tetrahydrofolate (methyl-THF) or other desired products, wherein the microorganism lacks the ability to convert CO or CO2 and H2 to acetyl-CoA or methyl-THF in the absence of the one or more exogenous proteins. For example, the microbial organism can contain at least one exogenous nucleic acid encoding an enzyme or protein in an acetyl-CoA pathway. The microbial organism is capable of utilizing synthesis gases comprising CO, CO2 and/or H2, alone or in combination with methanol, to produce acetyl-CoA.
    Type: Application
    Filed: May 12, 2011
    Publication date: September 15, 2011
    Applicant: GENOMATICA, INC.
    Inventors: Mark J. Burk, Christophe H. Schilling, Anthony P. Burgard, John D. Trawick
  • Patent number: 8012717
    Abstract: A method for producing cladribine (2-chloro-2? deoxyadenosine) comprising the steps of: a) reaction of 2-deoxyuridine with 2-chloroadenine, in the presence of uridine phosphorylase (UPase) and purine nucleoside phosphorylase (PNPase) in an aqueous reaction medium possibly containing up to 40% v/v of an aprotic dipolar solvent, to obtain cladribine dissolved in said reaction medium; b) isolation of the cladribine by precipitation by means of concentration and alkalinisation of the reaction medium up to pH 11.5-12.5.
    Type: Grant
    Filed: December 14, 2007
    Date of Patent: September 6, 2011
    Assignee: Explora Laboratories S.A.
    Inventors: Gabriele Zuffi, Simone Monciardini
  • Patent number: 8008007
    Abstract: S-Adenosyl-L-methionine analogs of formula (I): are disclosed wherein R comprises a carbon-carbon double bond, carbon-sulfur double bond, carbon-nitrogen double bond, -a carbon-carbon triple bond, carbon-nitrogen triple bond or an aromatic carbocyclic or heterocyclic system in ?-position to the sulfonium center, X{circle around (?)} is an organic or inorganic anion carrying one or more negative charges, Z is —CR1R2—, —O—, —S— or —NR3— and R1, R2 and R3 are independently selected from H, D and C1—C alkyl; as well as complexes with a methyltransferase, pharmaceutical compositions, methods for modifying a biomolecule, and methods for detecting sequences specific methylation of biomolecules.
    Type: Grant
    Filed: October 11, 2007
    Date of Patent: August 30, 2011
    Assignee: RWTH Aachen
    Inventors: Elmar Weinhold, Christian Dalhoff, Saulius Klimasauskas, Grazvydas Lukinavicius
  • Patent number: 7955835
    Abstract: The present invention relates to a process for producing compounds of formula (I) and (VII); said process comprising the steps of: a) subjecting a compound of formula (II) to an enzymatic diastereomeric resolution in the presence of a suitable amount of enzyme chosen from Pig Liver Esterase or Porcine Pancreatic Lipase b) recovering said compounds of formula (I) and (VII). The invention also provides a process for producing compounds of formula (III) and (X); said process comprising the steps of: a) subjecting a compound of formula (IV) to an enzymatic diastereomeric resolution in the presence of a suitable amount of enzyme chosen from Candida Antarctica “A” lipase, Candida Antarctica “B” lipase, Candida Lypolitica Lipase or Rhizomucor Miehei Lipase b) recovering said compound of formula (III) and (X).
    Type: Grant
    Filed: November 18, 2003
    Date of Patent: June 7, 2011
    Assignee: SHIRE Canada Inc.
    Inventors: Alex Cimpoia, James Joseph LaLonde
  • Publication number: 20110020875
    Abstract: Provided herein are polypeptides having hydrogenase activity. The polypeptide may be multimeric, and may have hydrogenase activity of at least 0.05 micromoles H2 produced min?1 mg protein?1. Also provided herein are polynucleotides encoding the polypeptides, genetically modified microbes that include polynucleotides encoding one or more subunits of the multimeric polypeptide, and methods for making and using the polypeptides.
    Type: Application
    Filed: December 5, 2008
    Publication date: January 27, 2011
    Applicant: University of Georgia Research Foundation, Inc.
    Inventors: Michael W.W. Adams, Francis E. Jenney, JR., Junsong Sun, Robert C. Hopkins
  • Publication number: 20110003342
    Abstract: A microorganism of the genus Corynebacterium having the ability to produce inosine in which the inosine catabolic pathway is blocked and that has a leaky adenine auxotrophic phenotype and further has a leaky guanine auxotrophic phenotype and a method of producing inosine, the method including culturing the microorganism of the genus Corynebacterium are disclosed.
    Type: Application
    Filed: January 2, 2009
    Publication date: January 6, 2011
    Applicant: CJ CHEILJEDANG CORP.
    Inventors: Chul Ha Kim, Jong Soo Choi, Jeong Hwan Kim, Hyoung Seok Kim, Jung Gun Kwon, Tae Min Ahn, Soo Youn Hwang, Jae Ick Sim, Min Ji Baek, Na Ra Kwon, Hye Jin Choi
  • Patent number: 7858351
    Abstract: The invention discloses a series of Methanococcus jannaschii S-adenosylmethionine synthetase mutants with improved thermostability and high catalytic activity obtained by using gene mutation technique, characterized in that these mutants refer to an enzyme using Sequence 2 in the Sequence Listing as the reference sequence and contains at least one mutation at position 102, position 93, position 230, and position 357 and has a catalytic activity at least 70% higher than that of the wild-type S-adenosylmethionine synthetase using adenosine triphosphate (ATP) and methionine as substrates. These S-adenosylmethionine synthetase mutants can be used in the production of S-adenosylmethionine.
    Type: Grant
    Filed: August 30, 2007
    Date of Patent: December 28, 2010
    Assignee: Geneharbor ( Hong Kong) Technologies, Ltd.
    Inventors: Rongzhao Fu, Tianzuo Zhang, Jun Wang
  • Publication number: 20100291632
    Abstract: A method for producing cladribine (2-chloro-2?-deoxyadenosine) comprising the steps of: a) reaction of 2-deoxyuridine with 2-chloroadenine, in the presence of uridine phosphorylase (UPase) and purine nucleoside phosphorylase (PNPase) in an aqueous reaction medium possibly containing up to 40% v/v of an aprotic dipolar solvent, to obtain cladribine dissolved in said reaction medium; b) isolation of the cladribine by precipitation by means of concentration and alkalinisation of the reaction medium up to pH 11.5-12.5.
    Type: Application
    Filed: December 14, 2007
    Publication date: November 18, 2010
    Applicant: EXPLORA Laboratories S.A.
    Inventors: Gabriele Zuffi, Simone Monciardini
  • Patent number: 7785839
    Abstract: This application provides a process for preparing enantiomerically pure ?-D-dioxolane nucleosides. In particular, a new synthesis of (?)-DAPD, suitable for large scale development, is described. In one embodiment the invention provides a process for preparing a substantially pure ?-D- or ?-L-1,3-dioxolane nucleosides comprising a) preparing or obtaining an esterified 2,2-dialkoxy ethanol; b) cyclizing the esterified 2,2-dialkoxy ethanol with glycolic acid to obtain a 1,3-dioxolane lactone; c) resolving the 1,3-dioxolane lactone to obtain a substantially pure D- or L-lactone; d) selectively reducing and activating the D- or L-chiral lactone to obtain a substantially pure D- or L-1,3-dioxolane; e) coupling the D- or L-1,3-dioxolane to an activated and/or protected purine or pyrimidine base; and f) optionally purifying the nucleoside to obtain a substantially pure protected ?-D- or ?-L-1,3-dioxolane nucleoside.
    Type: Grant
    Filed: February 3, 2005
    Date of Patent: August 31, 2010
    Assignee: Emory University
    Inventors: Marcos Sznaidman, George R. Painter, Merrick R. Almond, Darryl G. Gleary, Amir Pesyan
  • Patent number: 7785841
    Abstract: The disclosed nucleic acid primer sets, used in combination with quantitative amplification (PCR) of tissue cDNA, can indicate the presence of specific proteases in a tissue sample. Specifically, the present invention relates to expression of PUMP-1 protease (matrix metalloprotease 7). The detected proteases are themselves specifically over-expressed in certain cancers, and their presence may serve for early detection of associated ovarian and other malignancies, and for the design of interactive therapies for cancer treatment.
    Type: Grant
    Filed: December 15, 2004
    Date of Patent: August 31, 2010
    Assignee: Board of Trustees of the University of Arkansas
    Inventor: Timothy J. O'Brien
  • Patent number: 7776566
    Abstract: A microorganism which has a gene encoding an enzyme in which feedback inhibition is desensitized by substitution of one or two amino acids in PRPP amidotransferase encoded by purF of Escherichia coli, a gene encoding a protein which is an inactivated repressor of purine nucleotide biosynthesis encoded by purR, a gene encoding an enzyme which is inactivated purine nucleoside phosphorylase encoded by deoD, a gene encoding an enzyme which is inactivated succinyl-AMP synthase encoded by purA, a gene encoding an enzyme which is inactivated 6-phosphogluconate dehydrase encoded by edd, a gene encoding an enzyme which is inactivated phosphoglucose isomerase encoded by pgi and like is bred and a purine nucleoside is produced by culturing the microorganism.
    Type: Grant
    Filed: March 5, 2007
    Date of Patent: August 17, 2010
    Assignee: Ajinomoto Co., Inc.
    Inventors: Hiroshi Matsui, Hisashi Kawasaki, Megumi Shimaoka, Yasuhiro Takenaka, Osamu Kurahashi
  • Publication number: 20100081173
    Abstract: The present invention relates to a microorganism producing inosine, which is one of purine nucleoside, an important material for 5?-inosinic acid synthesis, and method for producing inosine using the same. More particularly, the present invention relates to a recombinant microorganism of Corynebacterium genus producing inosine at high concentration by inactivating the gene encoding nucleoside hydrolase II and by enhancing the expression of the gene encoding 5?-nucleotidase, which still retains the characteristics of Corynebacterium ammoniagenes CJIP2401 (KCCM-10610).
    Type: Application
    Filed: January 15, 2008
    Publication date: April 1, 2010
    Applicant: CJ CHEILJEDANG CORPORATION
    Inventors: Young-hoon Park, Kwang-myung Cho, Hee-jong Lee, Jin-nam Lee
  • Publication number: 20100047874
    Abstract: A purine-derived substance is produced by culturing a bacterium belonging to the genus Bacillus which has an ability to produce a purine-derived substance and has been modified so that enzymatic activity of transaldolase is decreased in a medium to cause accumulation of a purine-derived substance in the medium or cells, and collecting the purine-derived substance from the medium or cells.
    Type: Application
    Filed: October 21, 2008
    Publication date: February 25, 2010
    Inventors: Takayuki Asahara, Kiyoshi Matsuno, Yukiko Mori
  • Patent number: 7648967
    Abstract: The present invention relates to methods of treating viral disease using mutagenic nucleoside analogs. In particular, the invention provides isoguanosine nucleosides and derivatives thereof as well a method of increasing the mutation rate of a virus such as bovine viral diarrhea virus (BVDV) and hepatitis C virus (HCV).
    Type: Grant
    Filed: February 13, 2006
    Date of Patent: January 19, 2010
    Assignee: Koronis Pharmaceuticals, Incorporated
    Inventors: Ling Li, Alexander Gall, Richard Daifuku
  • Publication number: 20090298127
    Abstract: A method is provided for producing a purine nucleoside, such as inosine and guanosine, and a method for producing a 5?-purine nucleotide such as 5?-inosinic acid or 5?-guanylic acid, using a bacterium belonging to the either genus Escherichia or genus Bacillus, wherein purine nucleoside productivity of said bacterium is enhanced by enhancing an activity of a protein encoded by the yeaS (leuE) gene.
    Type: Application
    Filed: June 2, 2009
    Publication date: December 3, 2009
    Inventors: EKATERINA ALEKSANDROVNA KUTUKOVA, NATALIA PAVLOVNA ZAKATAEVA, VITALY ARKADIEVICH LIVSHITS
  • Publication number: 20090286284
    Abstract: A method of purifying cytidine diphosphate choline, which comprises contacting a cytidine diphosphate choline solution containing a nucleic acid analogue and having a pH of not less than 0.5 and not more than 5.0 with an H-type strongly acidic cation exchange resin, and eluting cytidine diphosphate choline adsorbed onto the resin with water or an aqueous solution having an ion concentration of not more than 0.1 mol/L to separate and purify the cytidine diphosphate choline.
    Type: Application
    Filed: August 10, 2006
    Publication date: November 19, 2009
    Applicant: KYOWA HAKKO KOGYO CO., LTD.
    Inventors: Hideki Murata, Tsuyoshi Mokudai, Michio Shiomi
  • Publication number: 20090269812
    Abstract: The invention provides a method of producing a chemical product through continuous fermentation which includes filtering a culture of a microorganism or cultured cells with a separation membrane to recover a product from a filtrate and simultaneously retaining a nonfiltered fluid in, or refluxing it to, the culture, and adding fermentation materials to the culture, wherein a porous membrane having an average pore size of 0.01 ?m or more to less than 1 ?m is used as the separation membrane and the filtration is conducted with a transmembrane pressure difference in the range of 0.1 to 20 kPa. According to this method, the fermentation productivity of the chemical product can be largely elevated at high stability and a low cost.
    Type: Application
    Filed: February 16, 2007
    Publication date: October 29, 2009
    Applicant: Toray Industries, Inc , a corporation of Japan
    Inventors: Hideki Sawai, Katsushige Yamada, Takashi Mimitsuka, Kenji Sawai, Tetsu Yonehara, Yohito Ito, Masahiro Henmi
  • Publication number: 20090203080
    Abstract: The invention discloses a series of Methanococcus jannaschii S-adenosylmethionine synthetase mutants with improved thermostability and high catalytic activity obtained by using gene mutation technique, characterized in that these mutants refer to an enzyme using Sequence 2 in the Sequence Listing as the reference sequence and contains at least one mutation at position 102, position 93, position 230, and position 357 and has a catalytic activity at least 70% higher than that of the wild-type S-adenosylmethionine synthetase using adenosine triphosphate (ATP) and methionine as substrates. These S-adenosylmethionine synthetase mutants can be used in the production of S-adenosylmethionine.
    Type: Application
    Filed: August 30, 2007
    Publication date: August 13, 2009
    Applicant: GENEHARBOR (HONG KONG) TECHNOLOGIES LTD.
    Inventors: Rongzhao Fu, Tianzuo Zhang, Jun Wang