Preparing Nitrogen-containing Saccharide Patents (Class 435/84)
  • Patent number: 10301658
    Abstract: The present invention relates to a method for in vivo production of glycosaminoglycans (GAG), by metabolic engineering in a genetically modified cell. In a method according to the invention, said cell is genetically modified in order to express the genes coding for the enzymes that are suitable for synthesizing GAG from an exogenous precursor, preferably internalized by the cell. According to one specific feature, the present invention relates to a method for producing chondroitin or heparosan in a genetically modified bacterial cell, from an exogenous beta-galactoside precursor, preferably internalized by the cell. According to another specific feature, the present invention relates to the use of an Escherichia coli cell comprising at least the genes glcA-T, kfoC, kfiD and wbpP for the production of chondroitin.
    Type: Grant
    Filed: January 23, 2015
    Date of Patent: May 28, 2019
    Assignees: CENTRE NATIONAL DE LA RECHERCHE SCIENTIFIQUE (CNRS), UNIVERSITE GRENOBLE ALPES
    Inventor: Bernard Priem
  • Patent number: 10292936
    Abstract: Covalently modified alginate polymers, possessing enhanced biocompatibility and tailored physiochemical properties, as well as methods of making and use thereof, are disclosed herein. The covalently modified alginates are useful as a matrix for the encapsulation and transplantation of cells. Also disclosed are high throughput methods for the characterizing the biocompatibility and physiochemical properties of modified alginate polymers.
    Type: Grant
    Filed: May 5, 2017
    Date of Patent: May 21, 2019
    Assignees: Massachusetts Institute of Technology, The Children's Medical Center Corporation
    Inventors: Arturo J. Vegas, Minglin Ma, Kaitlin M Bratlie, Daniel G. Anderson, Robert S. Langer
  • Patent number: 10093936
    Abstract: The present invention provides a recombinant Bacillus subtilis for producing acetylglucosamine and construction method thereof. The recombinant Bacillus subtilis is obtained by deletion of glmS ribozyme of Bacillus subtilis for regulating expression of glucosamine synthase, and insertion of a terminator and a constitutive promoter. The method comprises constructing a deleting cassette of a glmS ribozyme encoding gene, which includes an upstream homologous fragment, a resistance gene, a terminator sequence, a constitutive promoter sequence and a downstream homologous fragment in sequence; and transforming the deleting cassette into Bacillus subtilis, to obtain the recombinant Bacillus subtilis. In the invention, glucosamine synthase gene (glmS) ribozyme is deleted by homologous recombination, and in host cells, GlcN6P feedback inhibition of expression of glucosamine synthase gene glmS is blocked, and the accumulation of acetylglucosamine is improved.
    Type: Grant
    Filed: December 24, 2016
    Date of Patent: October 9, 2018
    Assignee: JIANGNAN UNIVERSITY
    Inventors: Long Liu, Jian Chen, Guocheng Du, Jianghua Li, Tengfei Niu
  • Patent number: 9783838
    Abstract: The present invention provides novel methods for preparing glycosylated molecules such as oligosaccharides, glycolipids, and glycoproteins/peptides. Novel sialyltransferases are also disclosed. The method includes forming a reaction mixture containing an acceptor molecule, a donor substrate having a sugar moiety and a nucleotide, and a sialyltransferase selected from PmST3 (SEQ ID NO:7) and certain variants thereof. The reaction mixture is formed under conditions sufficient to transfer the sugar moiety from the donor substrate to the acceptor molecule, thereby forming the glycosylated molecule. In some embodiments, the acceptor molecule is selected from a natural product, an oligosaccharide, a glycoprotein, and a glycolipid. In some embodiments, the donor substrate is formed via conversion of a suitable hexosamine derivative to a cytidine 5?-monophosphate(CMP)-sialic acid in a one-pot reaction mixture containing asialic acid aldolase and a CMP-sialic acid synthetase.
    Type: Grant
    Filed: November 7, 2012
    Date of Patent: October 10, 2017
    Assignee: The Regents of the University of California
    Inventors: Xi Chen, Vireak Thon, Hai Yu
  • Patent number: 9758803
    Abstract: The invention provides compositions and methods for engineering bacteria to produce sialylated and N-acetylglucosamine-containing oligosaccharides, and the use thereof in the prevention or treatment of infection.
    Type: Grant
    Filed: March 14, 2014
    Date of Patent: September 12, 2017
    Assignee: Glycosyn LLC
    Inventors: Massimo Merighi, Matthew Ian Heidtman, John M. McCoy
  • Patent number: 9562249
    Abstract: The present invention is directed to an innate DNA sequence within the complete genome sequence of Actinoplanes sp. SE50/110 which resembles the structure of an actinomycete integrative and conjugative element (AICE). Related AICEs were used for establishing genetic manipulation tools for other bacteria in the past. In this document, we describe the unique features of the specific AICE found in Actinoplanes sp. SE50/110 which are clearly distinct from any other known AICE as a whole, but share minor parts with varying sequence similarity with other characterized AICEs from other species.
    Type: Grant
    Filed: November 12, 2015
    Date of Patent: February 7, 2017
    Assignee: BAYER INTELLECTUAL PROPERTY GMBH
    Inventors: Andreas Klein, Klaus Selber, Hermann Wehlmann, Winfried Rosen, Alfred Pühler, Patrick Schwientek, Jörn Kalinowski, Udo Wehmeier
  • Patent number: 9217154
    Abstract: The present invention is directed to an innate DNA sequence within the complete genome sequence of Actinoplanes sp. SE50/110 which resembles the structure of an actinomycete integrative and conjugative element (AICE). Related AICEs were used for establishing genetic manipulation tools for other bacteria in the past. In this document, we describe the unique features of the specific AICE found in Actinoplanes sp. SE50/110 which are clearly distinct from any other known AICE as a whole, but share minor parts with varying sequence similarity with other characterized AiCEs from other species.
    Type: Grant
    Filed: December 4, 2012
    Date of Patent: December 22, 2015
    Assignee: BAYER INTELLECTUAL PROPERTY GMBH
    Inventors: Andreas Klein, Klaus Selber, Hermann Wehlmann, Winfried Rosen, Alfred Pühler, Patrick Schwientek, Jörn Kalinowski, Udo Wehmeier
  • Publication number: 20150147783
    Abstract: The present invention relates to an isolated mutant eubacterium comprising at least one mutation resulting in a substitution of at least one amino acid in the beta-subunit of the RNA-polymerase encoded for by the rpoB-gene providing an altered production of a product of interest when said production of a product of interest is compared to the production of the same product in an isogenic wild type strain grown at identical conditions, wherein the substitution of at least one amino acid occurs at any of positions 469, 478, 482, 485, or 487 of SEQ ID NO:2, or at the equivalent positions in any eubacterial RNA-polymerase beta-subunit family member. Another aspect of the invention relates to a process for producing at least one product of interest in a mutant eubacterium and to a use of the mutant eubacterium according to the invention for producing at least one product of interest.
    Type: Application
    Filed: February 9, 2015
    Publication date: May 28, 2015
    Inventors: Steen Troels Joergensen, Jens Toenne Andersen, Niels Banke, Preben Nielsen
  • Publication number: 20150111255
    Abstract: Disclosed is a process comprising (1) forming an aqueous mixture comprising a microbial composition and solid chitin, wherein said microbial composition comprises one or more microbes that produce chitin digesting enzymes; and (2) fermenting the mixture for a time sufficient to enzymatically digest all or part of the chitin to form a fermented mixture comprising chitosan and glucosamine. In some embodiments, the chitin is derived from the biodegradation of chitin containing marine Arthropods. In other embodiments, the chitin is obtained from chitin containing fungi, filamentous fungi and yeast which is extracted via a chemical process. In yet another embodiment, the chitin is obtained by the biodegradation of chitin containing fungi, filamentous fungi, yeast and/or insects, preferably using HQE for the digestion.
    Type: Application
    Filed: December 19, 2014
    Publication date: April 23, 2015
    Applicant: Agrinos AS
    Inventor: Jaime López-Cervantes
  • Patent number: 8999671
    Abstract: The present invention relates to eukaryotic host cells which have been modified to produce sialylated glycoproteins by the heterologous expression of a set of glycosyltransferases, including sialyltransferase and/or trans-sialidase, to become host-strains for the production of mammalian, e.g., human therapeutic glycoproteins. Novel eukaryotic host cells expressing a CMP-sialic acid biosynthetic pathway for the production of sialylated glycoproteins are also provided. The invention provides nucleic acid molecules and combinatorial libraries which can be used to successfully target and express mammalian enzymatic activities (such as those involved in sialylation) to intracellular compartments in a eukaryotic host cell. The process provides an engineered host cell which can be used to express and target any desirable gene(s) involved in glycosylation.
    Type: Grant
    Filed: July 20, 2012
    Date of Patent: April 7, 2015
    Assignee: Glycofi, Inc.
    Inventor: Stephen R. Hamilton
  • Patent number: 8986973
    Abstract: Provided is a Streptococcus dysgalactiae ID9103 strain having accession number KCTC11818BP, and a method of producing hyaluronic acid by culturing the strain to produce hyaluronic acid having an average molecular weight of 10,000,000 Da or more.
    Type: Grant
    Filed: March 26, 2014
    Date of Patent: March 24, 2015
    Assignee: Ildong Pharm Co., Ltd.
    Inventors: Dae-Jung Kang, Jong-Hyuk Im, Tae-Yoon Kim, Jae-Hoon Kang
  • Patent number: 8975043
    Abstract: Novel enzymes, processes and antigenic structures useful in producing vaccines and compounds useful in combating gram-negative bacteria are described. Enzymes were isolated from the slime mold Dictyostelium discoideum and used to specifically degrade lipopolysaccharide (LPS). Enzymatic degradation permits residues of the LPS molecule, including immunogenic epitopes of the core oligosaccharide portion of the LPS, to remain unmodified during this enzymatic removal of fatty acids from the lipid A region of the LPS molecule.
    Type: Grant
    Filed: November 23, 2006
    Date of Patent: March 10, 2015
    Assignee: National Research Council of Canada
    Inventors: Andrew Cox, Dhamodharan Neelamegan, Ian Schoenhofen, Frank St. Michael, James Richards
  • Publication number: 20150031085
    Abstract: The present invention provides a novel leech HAase and a method of producing low-molecular-weight HA oligosaccharides using the leech HAase. This invention successfully cloned the first leech HAase gene and provides a method for high-level expression of the leech HAase gene. By controlling the incubation condition, different HA oligosaccharides, particularly HA4, HA6, HA8 and HA10, can be selectively generated using the leech HAase. The large-scale expression of the leech HAase and the enzymatic production of specific HA oligosaccharides are not only useful for the cosmetic, healthcare and the medical industries but also can be a great help to polysaccharides chemical synthesis and cancer research.
    Type: Application
    Filed: April 12, 2014
    Publication date: January 29, 2015
    Applicant: JIANGNAN UNIVERSITY
    Inventors: Jian Chen, Guocheng Du, Zhen Kang, Peng Jin
  • Publication number: 20150010949
    Abstract: The present invention relates to an aqueous carbohydrate based binder composition, comprising a carbohydrate component and an amine component, wherein the carbohydrate component comprises one or more pentose sugars, as well as to a method of its production.
    Type: Application
    Filed: September 2, 2012
    Publication date: January 8, 2015
    Applicant: Knauf Insulation
    Inventors: Roger Jackson, Carl Hampson, James Robinson, Benedicte Pacorel
  • Patent number: 8927234
    Abstract: It is intended to provide a simple method for producing hyaluronic acid at a high yield. Further, it is also intended to provide a method for producing hyaluronic acid in a short period of time. The invention provides a method for producing hyaluronic acid including a step of culturing a microorganism having the capability to produce hyaluronic acid and a step of adding glutamine and arginine to a culture medium during late logarithmic growth phase of the microorganism.
    Type: Grant
    Filed: July 25, 2008
    Date of Patent: January 6, 2015
    Assignee: Denki Kagaku Kogyo Kabushiki Kaisha
    Inventors: Masamichi Hashimoto, Teruaki Kakema, Kenji Fujii, Masahisa Ikemi
  • Publication number: 20140356912
    Abstract: The present invention relates to the the cell-based production of bacterial nonulosonates and their biosynthetic precursors. Specifically, the present invention provides recombinant cells for the production of pseudaminic acid, legionaminic acid, UDP-2,4-diacetamido-2,4,6-trideoxy-?-L-altropyranose, and UDP-2,4-diacetamido-2,4,6-trideoxy-?-D-glucopyranose. Methods for producing the sugars are also provided.
    Type: Application
    Filed: July 28, 2014
    Publication date: December 4, 2014
    Inventors: Christopher N. Boddy, Susan M. Logan, Benjamin R. Lundgren, Ian C. Schoenhofen, Dennis M. Whitfield
  • Publication number: 20140349346
    Abstract: The present invention is directed to an innate DNA sequence within the complete genome sequence of Actinoplanes sp. SE50/110 which resembles the structure of an actinomycete integrative and conjugative element (AICE). Related AICEs were used for establishing genetic manipulation tools for other bacteria in the past. In this document, we describe the unique features of the specific AICE found in Actinoplanes sp. SE50/110 which are clearly distinct from any other known AICE as a whole, but share minor parts with varying sequence similarity with other characterized AiCEs from other species.
    Type: Application
    Filed: December 4, 2012
    Publication date: November 27, 2014
    Applicant: BAYER INTELLECTUAL PROPERTY GMBH
    Inventors: Andreas Klein, Klaus Selber, Hermann Wehlmann, Winfried Rosen, Alfred Pühler, Patrick Schwientek, Jörn Kalinowski, Udo Wehmeier
  • Publication number: 20140349347
    Abstract: Method of making NANA from NAM, which may itself be made from fructose. The NAM is treated with pyruvic acid or a pyruvate in the presence of a NANA aldolase having at least 70% sequence similarity or homology to the amino acid sequence of SEQ. ID. NO. 1.
    Type: Application
    Filed: October 10, 2012
    Publication date: November 27, 2014
    Applicant: Glycom A/S
    Inventors: Andreas Schroven, Gyula Dekany, Ionnis Vrasidas
  • Patent number: 8883452
    Abstract: A method for the production of heparosan from fermentation culture of E. coli K5 suitable for industrial production, exhibiting superior yield and purity, smaller culture volumes, faster growth, and lower costs.
    Type: Grant
    Filed: August 30, 2010
    Date of Patent: November 11, 2014
    Assignee: Rensselaer Polytechnic Institute
    Inventors: Zhenyu Wang, Robert J. Linhardt, Jonathan S. Dordick, Ujjwal Bhaskar
  • Patent number: 8852891
    Abstract: The present invention relates to a novel N-acetylglucosamine-2-epimerase and a method for preparing CMP-N-acetylneuraminic acid, more specifically, relates to a N-acetylglucosamine-2-epimerase derived from Bacteroides fragilis NCTC 9343, and a method for preparing CMP-N-acetylneuraminic acid using said N-acetylglucosamine-2-epimerase. According to the present invention, CMP-N-acetylneuraminic acid can be produced economically in a large amount through a one-step reaction using cytidine monophosphate and N-acetyl-D-glucosamine which are inexpensive substrates.
    Type: Grant
    Filed: December 7, 2007
    Date of Patent: October 7, 2014
    Assignee: Gene Chem, Inc.
    Inventors: Jin Suk Woo, Jae Kyung Sohng, Byung Gee Kim, Sun Youp Kang, Dea Hee Kim, Kyoung Soon Jang, Ji Young Yang, Young Soo Jung, Won Min Seo, Tae Geon Gil, Sang Hee Shim, In Kang Hur
  • Publication number: 20140296505
    Abstract: The invention relates to the field of recombinant DNA technology for the production of chondroitin, including the production of chondroitin sulfate via a combination of recombinant bacterial fermentation and post-fermentation sulfation.
    Type: Application
    Filed: February 20, 2014
    Publication date: October 2, 2014
    Applicants: Seikagaku Corporation, DSM IP ASSETS B.V.
    Inventors: Daniel H. DOHERTY, Craig A. WEAVER, Kentaro MIYAMOTO, Toshikazu MINAMISAWA
  • Publication number: 20140235575
    Abstract: The present invention provides a one-pot multi-enzyme method for preparing UDP-sugars from simple sugar starting materials. The invention also provides a one-pot multi-enzyme method for preparing oligosaccharides from simple sugar starting materials.
    Type: Application
    Filed: January 21, 2014
    Publication date: August 21, 2014
    Applicant: THE REGENTS OF THE UNIVERSITY OF CALIFORNIA
    Inventors: Xi Chen, Hai Yu, Yanhong Li, Yi Chen, Jingyao Qu, Musleh M. Muthana, Kam Lau, Lars Bode
  • Publication number: 20140206040
    Abstract: Provided is a Streptococcus dysgalactiae ID9103 strain having accession number KCTC11818BP, and a method of producing hyaluronic acid by culturing the strain to produce hyaluronic acid having an average molecular weight of 10,000,000 Da or more.
    Type: Application
    Filed: March 26, 2014
    Publication date: July 24, 2014
    Applicant: ILDONG PHARM CO., LTD.
    Inventors: Dae-Jung KANG, Jong-Hyuk IM, Tae-Yoon KIM, Jae-Hoon Kang
  • Patent number: 8778636
    Abstract: Processes for preparing pimecrolimus starting from ascomycin, exploiting the selectivity characteristics of the purified enzymatic systems particularly regarding the selective functionalization of the hydroxyl groups present in position 24 and 33 of ascomycin. Such method represents the first example of chemoenzymatic synthesis for preparing pimecrolimus.
    Type: Grant
    Filed: May 19, 2010
    Date of Patent: July 15, 2014
    Assignee: Euticals S.p.A.
    Inventors: Paride Grisenti, Shahrzad Reza Elahi, Elisa Verza
  • Patent number: 8771992
    Abstract: Chondroitin is produced by culturing a recombinant microorganism which is obtained by inactivation of a gene encoding an enzyme responsible for addition of fructose residues to the linear chondroitin polysaccharide in a microorganism producing a fructosylated derivative of chondroitin.
    Type: Grant
    Filed: November 13, 2013
    Date of Patent: July 8, 2014
    Assignee: GNOSIS S.p.A.
    Inventors: Antonio Trilli, Immacolata Busiello, Simona Daly, Francesca Bagatin
  • Publication number: 20140178912
    Abstract: An exemplary multimodal chromatographic medium of the invention includes one or more strong anion exchange, weak anion exchange, strong cation exchange and/or weak cation exchange binding sites in combination with one or more reverse phase and/or hydrophilic interaction chromatography binding site. In an exemplary embodiment, the sites interact with one or more glycans in a mixture of glycans in a manner that allows separation of glycans in the mixture and analysis of the glycan mixture. The media are incorporated into devices and systems for chromatographic analysis. Also provided are methods of using the multimodal media of the invention to analyze glycans.
    Type: Application
    Filed: December 21, 2012
    Publication date: June 26, 2014
    Inventors: Xiaodong LIU, Udayanath AICH, Christopher A. POHL
  • Patent number: 8759033
    Abstract: The present invention relates to genetically modified actinobacteria for the production of an enzyme having chitosanase activity. The genetically modified actinobacteria have a reduced (or abolished) activity of the CsnR polypeptide. Such reduced activity can be obtained by reducing the capacity of expressing the csnR gene, its corresponding transcript or expressing a dominant-negative CsnR polypeptide. Such genetically modified actinobacteria are less dependent (and, in some embodiment, totally independent) on the presence of chitosan in the culture medium for producing an enzyme having chitosanase activity. In addition, the genetically modified bacteria produce less proteases in the culture medium and ultimately provide a chitosanase end-product with higher purity.
    Type: Grant
    Filed: February 8, 2013
    Date of Patent: June 24, 2014
    Assignee: Socpra Sciences et Genie S.E.C.
    Inventors: Ryszard Brzezinski, Marie-Pierre Dubeau
  • Publication number: 20140147891
    Abstract: The present invention relates to a novel endoglycoceramidase whose hydrolytic activity has been substantially reduced or eliminated, such that the enzyme is useful for synthesis of glycolipids from a monosaccharide or oligosaccharide and a ceramide. More specifically, the endoglycoceramidase is a mutant version of a naturally occurring endoglycoceramidase, preferably comprising a mutation within the active site or the nucleophilic site of the enzyme and more preferably comprising a substitution mutation of the Glu residue within the active site or the nucleophilic site. Also disclosed are a method for generating the mutant endoglycoceramidase and a method for enzymatically synthesizing glycolipids using this mutant enzyme.
    Type: Application
    Filed: July 24, 2013
    Publication date: May 29, 2014
    Applicants: The University of British Columbia University- Industry Liaison Office, Seneb BioSciences, Inc.
    Inventors: Karl F. Johnson, Shawn Defrees, Stephen Withers, Mark Vaughan
  • Patent number: 8735102
    Abstract: The present invention relates to a recombinant Bacillus host cell containing a recombinant vector including a nucleic acid segment having a coding region segment encoding enzymatically active hyaluronan synthase (HAS). The recombinant Bacillus host cell is utilized in a method for producing hyaluronic acid (HA).
    Type: Grant
    Filed: July 5, 2011
    Date of Patent: May 27, 2014
    Assignee: The Board of Regents of the University of Oklahoma
    Inventors: Paul L. DeAngelis, Paul H. Weigel, Kshama Kumari
  • Patent number: 8722366
    Abstract: The present invention provides a novel polypeptide having a ?1,3-N-acetylglucosaminyltransferase activity; a method for producing the polypeptide; a DNA which encodes the polypeptide; a recombinant vector into which the DNA is inserted; a transformant comprising the recombinant vector; a method for producing a sugar chain or complex carbohydrate, using the polypeptide; a method for producing a sugar chain or complex carbohydrate, using the transformant; an antibody which recognizes the polypeptide; a method for screening a substance which changes the expression of the gene which encodes the polypeptide; and a method for screening a substance which changes the activity of the polypeptide.
    Type: Grant
    Filed: June 21, 2011
    Date of Patent: May 13, 2014
    Assignee: Kyowa Hakko Kirin Co., Ltd.
    Inventors: Katsutoshi Sasaki, Norihiko Shiraishi, Ayumi Natsume, Yoji Yamada, Satoshi Nakagawa, Susumu Sekine
  • Patent number: 8722365
    Abstract: A metabolically engineered E. coli strain which produces sialic acid and a method of making said strain. In the engineered E. coli cells, the nanT (sialic acid transporter) and nanA (sialic acid adolase) genes are inactivated, and the neuC and neuB genes of sialic acid biosynthesis in Neisseria meningitidis group B are introduced and overexpressed in the nanT? nanA? E. coli cell. In addition, the glucosamine synthase gene, glmS, of E. coli is co-overexpressed with neuB and neuC.
    Type: Grant
    Filed: September 26, 2007
    Date of Patent: May 13, 2014
    Assignee: Syracuse University
    Inventors: Christopher N. Boddy, Benjamin R. Lundgren
  • Publication number: 20140120584
    Abstract: This invention concerns a process for the co-production of glucosamine polymers (chitin, chitosan or any of its derivatives) and polymers containing glucose, mannose and/or galactose, by the high cell density fermentation of the yeast Pichia pastoris in a bioreactor under aerobic conditions, preferably using glycerol byproduct from the biodiesel industry as carbon source. Pure glycerol, pure methanol, glycerol-rich or methanol rich mixtures may also be used as carbon sources. The present invention also concerns P. pastoris fermentation process duly optimized for attaining high cell densities and high cell wall chitin content, as well as polymers containing glucose, mannose and/or galactose.
    Type: Application
    Filed: December 20, 2013
    Publication date: May 1, 2014
    Applicant: 73100 SETENTA E TRES MIL E CEM, LDA
    Inventors: Maria d'Ascencao Carvalho Fernandes De Miranda Reis, Rui Manuel Freitas Oliveira, Maria Filomena Andrade De Freitas, Barbara Ferreira Chagas, Ana Luisa Braga Da Cruz, Antonio Eduardo Pio Barbosa Pereira Da Cunha, Joao Jose Vazao Mano Clemente
  • Patent number: 8697398
    Abstract: The invention relates to the field of recombinant DNA technology for the production of chondroitin, including the production of chondroitin sulfate via a combination of recombinant bacterial fermentation and post-fermentation sulfation.
    Type: Grant
    Filed: March 1, 2011
    Date of Patent: April 15, 2014
    Assignees: DSM IP Assets B.V., Seikagaku Corporation
    Inventors: Daniel H. Doherty, Craig A. Weaver, Kentaro Miyamoto, Toshikazu Minamisawa
  • Publication number: 20140099673
    Abstract: The present invention relates to a method of producing hyaluronic acid (HA) in Escherichia coli and Bacillus megaterium through episomal plasmid vectors wherein the gene is under the control of strong promoter T7, preferably under the control of strong promoter T7 of bacteriophage T7, and a system for the selection of stable bacterial strains producing high levels of hyaluronic acid.
    Type: Application
    Filed: September 9, 2011
    Publication date: April 10, 2014
    Applicant: FIDIA FARMACEUTICI S.P.A.
    Inventors: Vincenza Corsa, Alessandro Negro, Sonia Bisicchia
  • Patent number: 8685674
    Abstract: A vector of the present invention has DNA encoding a protein or a product having the same effect as the protein, the protein containing an amino acid sequence from amino acid numbers 47 to 802 in SEQ. ID. NO:2. Expression of the DNA gives human chondroitin synthase. By using human chondroitin synthase, it is possible to produce a saccharide chain having a repeating disaccharide unit of chondroitin. The DNA or part thereof may be used as a probe for hybridization for the human chondroitin synthase.
    Type: Grant
    Filed: October 9, 2012
    Date of Patent: April 1, 2014
    Assignee: Glytech, Inc.
    Inventors: Kazuyuki Sugahara, Hiroshi Kitagawa
  • Patent number: 8685673
    Abstract: The present invention provides a method for in vitro producing an indole derivative in a one-pot reaction. The method for producing a rhamnosylated indolocarbazole compound includes the steps of transforming a plasmid carrying a gene encoding N-glycosyltransferase into a bacterial strain; expressing the gene encoding N-glycosyltransferase in the bacterial strain; lysing the bacterial strain to obtain a crude enzyme extract; and adding TDP-glucose, an indolocarbazole aglycone and a metal ion in the crude enzyme extract for performing an enzymatic reaction to form the rhamnosylated indolocarbazole compound. Alternatively, the method for producing an indole-3-carboxaldehyde analog includes the steps of transforming a plasmid carrying a gene encoding NokA of Nocardiopsis sp.
    Type: Grant
    Filed: August 2, 2010
    Date of Patent: April 1, 2014
    Assignee: National Chiao Tung University
    Inventor: Hsien-Tai Chiu
  • Publication number: 20140045218
    Abstract: A surfactant produced by reacting naturally occurring polysaccharides that are not water soluble with a hydrophilic substituent on a carboxylic portion of the polysaccharide. In a second reaction, the surfactant is further substituted on a hydroxylic portion with a hydrophobic or lipophilic substituent, so as to make the reaction product both water soluble and capable of attracting oily material that is hydrophobic to be removed from a substrate by cleaning in water. Methods of making the surfactant and the follow-on reaction product are described.
    Type: Application
    Filed: March 15, 2013
    Publication date: February 13, 2014
    Applicant: University of Massachusetts
    Inventors: Zarif Farhana Mohd Aris, Ryan M. Bouldin, Ramaswamy Nagarajan, Bridgette Budhlall, Vishal Bavishi
  • Publication number: 20140038235
    Abstract: The present invention relates to methods for producing a hyaluronic acid, comprising: (a) cultivating a Bacillus host cell under conditions suitable for production of the hyaluronic acid, wherein the Bacillus host cell comprises a nucleic acid construct comprising a hyaluronan synthase encoding sequence operably linked to a promoter sequence foreign to the hyaluronan synthase encoding sequence; and (b) recovering the hyaluronic acid from the cultivation medium. The present invention also relates to an isolated nucleic acid sequence encoding a hyaluronan synthase operon comprising a hyaluronan synthase gene and a UDP-glucose 6-dehydrogenase gene, and optionally one or more genes selected from the group consisting of a UDP-glucose pyrophosphorylase gene, UDP-N-acetylglucosamine pyrophosphorylase gene, and glucose-6-phosphate isomerase gene.
    Type: Application
    Filed: October 10, 2013
    Publication date: February 6, 2014
    Applicant: Novozymes Biopharma DK A/S
    Inventors: Alan Sloma, Regine Behr, William Widner, Stephen Brown, Maria Tang, David Sternberg
  • Publication number: 20130338101
    Abstract: There are provided methods for producing a hydrogel that is capable of adhesion of cells and which comprises enzymatically cross-linked conjugates of a hydrogel forming agent and a flavonoid, formed from a reaction using peroxide and peroxidase. Hydrogels produced by such methods and methods of using the hydrogels are also provided.
    Type: Application
    Filed: August 19, 2013
    Publication date: December 19, 2013
    Inventors: Motoichi Kurisawa, Fan Lee, Joo Eun Chung, Pui Yik Peggy Chan
  • Patent number: 8586332
    Abstract: The invention concerns the production by microbiological process of oligopolysaccharides of biological interest. More particularly, the invention concerns a method for synthesizing in vivo the oligopolysaccharides by internalization of an exogenous precursor in growing bacterial cells expressing adequate modifying and glycosylating genes.
    Type: Grant
    Filed: October 31, 2007
    Date of Patent: November 19, 2013
    Assignee: Centre National de la Recherche Scientifique (CNRS)
    Inventors: Eric Samain, Bernard Priem
  • Publication number: 20130302855
    Abstract: The present invention describes the DNA-sequence of the wild type genome as well as all genetic modifications which were introduced into the wild type-and further developed strains, based thereon. Thereby the first genotypic characterization of the developed strains, including the latest production strain, has been accomplished, accounting for the major part of the invention. Furthermore, on the basis of the determined DNA-sequences, potential genes were identified and account, combined with their functional annotation, for another part of the invention. In particular, the gene-and DNA-sequences, as well as protein-sequences derived there out, contribute to the invention which were affected by mutagenic modifications throughout the strain development process, potentially contributing to the increased production yield.
    Type: Application
    Filed: August 1, 2011
    Publication date: November 14, 2013
    Applicant: BAYER INTELLECTUAL PROPERTY GMBH
    Inventors: Klaus Selber, Bernhard Weingaertner, Hermann Wehlmann, Winfried Rosen, Alfred Puhler, Patrick Schwientek, Jörn Kalinowski, Udo Wehmeier
  • Patent number: 8574886
    Abstract: The present invention relates to methods for producing a hyaluronic acid, comprising: (a) cultivating a Bacillus host cell under conditions suitable for production of the hyaluronic acid, wherein the Bacillus host cell comprises a nucleic acid construct comprising a hyaluronan synthase encoding sequence operably linked to a promoter sequence foreign to the hyaluronan synthase encoding sequence; and (b) recovering the hyaluronic acid from the cultivation medium. The present invention also relates to an isolated nucleic acid sequence encoding a hyaluronan synthase operon comprising a hyaluronan synthase gene and a UDP-glucose 6-dehydrogenase gene, and optionally one or more genes selected from the group consisting of a UDP-glucose pyrophosphorylase gene, UDP-N-acetylglucosamine pyrophosphorylase gene, and glucose-6-phosphate isomerase gene.
    Type: Grant
    Filed: February 21, 2012
    Date of Patent: November 5, 2013
    Assignee: Novozymes, Inc.
    Inventors: Regine Behr, William Widner, Maria Tang, Stephen Brown, Leslie Naggiar, Linda Sternberg
  • Publication number: 20130288932
    Abstract: According to an embodiment, a method of providing a biopolymer comprises: cryodesiccating a broth, wherein the broth comprises: (A) a biopolymer, wherein the biopolymer is an exopolysaccharide; and (B) a liquid growth medium. According to certain embodiments, the biopolymer is produced by the fermentation of at least one carbohydrate via a microbe. According to an embodiment, the microbe is a bacterium. According to another embodiment, the microbe is a fungus.
    Type: Application
    Filed: April 27, 2012
    Publication date: October 31, 2013
    Applicant: HALLIBURTON ENERGY SERVICES, INC.
    Inventors: Andrew A.F. Mackenzie, Eric Davidson
  • Publication number: 20130266987
    Abstract: The invention relates to an isolated nucleic acid molecule comprising at least one promoter that is active in fungal cells of the trichoderma species, wherein a nucleic acid sequence encoding an N-acetylglucosamine-2-epimerase and/or an N-acetylneuraminic acid synthase is operatively bound to each promoter. The at least one promoter that is active in fungal cells is a constitutive promoter.
    Type: Application
    Filed: December 22, 2011
    Publication date: October 10, 2013
    Applicant: TECHNISCHE UNIVERSITÄT WIEN
    Inventors: Astrid Mach-Aigner, Robert Mach, Matthias G. Steiger
  • Patent number: 8551736
    Abstract: A microorganism of the genus Corynebacterium having the ability to produce inosine in which the inosine catabolic pathway is blocked and that has a leaky adenine auxotrophic phenotype and further has a leaky guanine auxotrophic phenotype and a method of producing inosine, the method including culturing the microorganism of the genus Corynebacterium are disclosed.
    Type: Grant
    Filed: January 2, 2009
    Date of Patent: October 8, 2013
    Assignee: CJ Cheiljedang Corp.
    Inventors: Chul Ha Kim, Jong Soo Choi, Jeong Hwan Kim, Hyoung Seok Kim, Jung Gun Kwon, Tae Min Ahn, Soo Youn Hwang, Jae Ick Sim, Min Ji Baek, Na Ra Kwon, Hye Jin Choi
  • Publication number: 20130261079
    Abstract: High molecular weight heparosan polymers are described, as are methods of producing and using the high molecular weight heparosan polymers.
    Type: Application
    Filed: April 1, 2013
    Publication date: October 3, 2013
    Applicant: THE BOARD OF REGENTS OF THE UNIVERSITY OF OKLAHOMA
    Inventors: Paul L. DeAngelis, Phillip Pummill, Regina C. Visser
  • Publication number: 20130210077
    Abstract: The present invention relates to genetically modified actinobacteria for the production of an enzyme having chitosanase activity. The genetically modified actinobacteria have a reduced (or abolished) activity of the CsnR polypeptide. Such reduced activity can be obtained by reducing the capacity of expressing the csnR gene, its corresponding transcript or expressing a dominant-negative CsnR polypeptide. Such genetically modified actinobacteria are less dependent (and, in some embodiment, totally independent) on the presence of chitosan in the culture medium for producing an enzyme having chitosanase activity. In addition, the genetically modified bacteria produce less proteases in the culture medium and ultimately provide a chitosanase end-product with higher purity.
    Type: Application
    Filed: February 8, 2013
    Publication date: August 15, 2013
    Applicant: SOCPRA SCIENCES ET GENIE S.E.C.
    Inventor: SOCPRA SCIENCES ET GENIE S.E.C.
  • Patent number: 8507227
    Abstract: The present invention relates to a method for the large scale in vivo synthesis of sialylated oligosaccharides, culturing a microorganism in a culture medium, optionally comprising an exogenous precursor such as lactose, wherein said microorganism comprises heterologous genes encoding a CMP-Neu5Ac synthetase, a sialic acid synthase, a GlcNAc-6-phosphate 2 epimerase and a sialyltransferase, and wherein the endogenous genes coding for sialic acid aldolase (NanA) and for ManNac kinase (NanK) have been deleted or inactivated. The invention also relates to these micoorganisms which are capable of producing internally activated sialic acid.
    Type: Grant
    Filed: March 7, 2007
    Date of Patent: August 13, 2013
    Assignee: Centre National de la Recherche Scientifique (CNRS)
    Inventor: Eric Samain
  • Publication number: 20130203980
    Abstract: For the first time, an O-specific polysaccharide antigen that is a Shigella Sonnei, phase I, exopolysaccharide has been produced and characterized, said exopolysaccharide being an authentic natural compound in the form of a bacterial capsular polysaccharide. The exopolysaccharide contains a non-toxic lipid component, namely non-hydroxylated fatty acids, and exhibits low pyrogenicity and high immunogenicity. Without using lipopolysaccharides as the source of production, an exopolysaccharide with a high degree of purity is produced from a liquid phase culture of S. sonnei bacteria by means of a workable industrial method with a high yield.
    Type: Application
    Filed: May 6, 2011
    Publication date: August 8, 2013
    Inventors: Petr Gennadievich Aparin, Vyacheslav Leonidovich Lvov, Stanislava Ivanovan Elkina, Marina Eduardovna Golovina, Vladimir Igorevich Shmigol
  • Publication number: 20130196381
    Abstract: The present invention relates to the cell-based production of bacterial nonulosonates and their biosynthetic precursors. Specifically, the present invention provides recombinant cells for the production of pseudaminic acid, legionaminic acid, UDP-2,4-diacetamido-2,4,6-trideoxy-?-L-altropyranose, and UDP-2,4-diacetamido-2,4,6-trideoxy-?-D-glucopyranose. Methods for producing the sugars are also provided.
    Type: Application
    Filed: April 20, 2011
    Publication date: August 1, 2013
    Applicants: UNIVERSITY OF OTTAWA, NATIONAL RESEARCH COUNCIL OF CANADA
    Inventors: Christopher N. Boddy, Susan M. Logan, Benjamin R. Lundgren, Ian C. Schoenhofen, Dennis M. Whitfield