Produced By The Action Of A Glycosyl Transferase (e.g., Alpha, Beta, Gamma-cyclodextrins By The Action Of Glycosyl Transferase On Starch, Etc.) Patents (Class 435/97)
Abstract: A process for producing a stevioside derivative, which comprises reacting stevioside with a .beta.-1,3- or .beta.-1,4-glycosyl sugar compound in aqueous solution or aqueous suspension in the presence of a microorganism or enzyme having .beta.-1,3- or .beta.1,4-glycosyl transferring activity thereby to form .beta.-1,3- or .beta.-1,4-glycosyl stevioside.
Type:
Grant
Filed:
February 25, 1983
Date of Patent:
May 20, 1986
Assignees:
Dainippon Ink and Chemicals, Inc., Dic Fine Chemicals, Inc.
Abstract: New .alpha.-glycosyl glycyrrhizins bearing two or more .alpha.-glucose residues are prepared. Such .alpha.-glycosylation is carried out by subjecting an aqueous solution of glycyrrhizin (or a salt thereof) and an amylaceous substance (e.g. starch or cyclodextrin) to the enzymatic action of an .alpha.-glycosyl transferase (e.g. cyclodextrin glucanotrasferase). The .alpha.-glycosyl glycyrrhizins are low-caloric, low-cariogenic, mild, non-bitter, non-lingering sweeteners which may be advantageously incorporated into foods, beverages, cosmetics, dentifrices and drugs.
Abstract: A maltodextrin phosphorylase limit dextrin in the presence of maltodextrin phosphorylase and inorganic phosphate, is used as a substrate for alpha-amylase, which initiates a series of enzymatic reactions resulting in a chromogen response which can be used to measure the concentration of alpha-amylase in a body fluid. A novel limit dextrin and its preparation also are described.
Type:
Grant
Filed:
September 1, 1981
Date of Patent:
March 19, 1985
Assignee:
CooperBiomedical, Inc.
Inventors:
Paul T. Nix, Rebecca D. Goldfarb, Linda J. Stong, Lorraine E. Sulick, Ramesh C. Trivedi, Stanley W. Morgenstern
Abstract: A process for producing cyclodextrin from starch in which an aqueous solun of starch is subjected to the action of an active cyclodextrin glycosyltransferase and the reaction mixture containing cyclodextrin, starch degradation products and active enzyme is continuously subjected to an ultrafiltration process to effect passage of the formed cyclodextrin through the membrane, while retaining substantially all of the other starch degradation products and active enzyme, thus permitting more cyclodextrin to be formed in the retentate, which will then pass the membrane, collecting the aqueous solution of cyclodextrin and recovering the cyclodextrin.
Type:
Grant
Filed:
September 20, 1982
Date of Patent:
October 16, 1984
Assignee:
Proefstation voor Aardappelverweking-TNO en Cooperatieve Verkoop en Produktievereniging van Aardappelmeel en Derivaten AVERE B.A.
Inventors:
Hendrik Hokse, Frederik S. Kaper, Jacob T. Wijpkema
Abstract: The present invention relates to a process for the production of branching enzyme, and a method for improving the qualities of food products therewith. According to the present invention, a large amount of branching enzyme with a high specific activity can be easily obtained, and employment of the branching enzyme in food processing remarkably improves the qualities of food products without changing the desirable inherent properties of their amylaceous constituent(s); thus, said food products retain their qualities over a long period of time, prolonging extremely their shelf lives.
Abstract: Enzyme-containing cells immobilized in an alginate gel are stabilized by contacting the gel with glycerol in a ratio of cellstoglycerol of 2:1 to 1:5. The enzyme-containing cells preferably convert sucrose to isomaltulose.
Abstract: Certain glucans modify the biosynthetic route of extracellular polysaccharides involved in dental plaque development causing critical loss of adhesiveness. They interfere with the biosynthesis of cell-bound polysaccharides reducing and preventing agglutination of cells. They thus aid in the prevention of dental plaque formation or concomitant dental caries and periodontal disease.
Abstract: This invention relates to a process for producing .gamma.-cyclodextrin without using any organic solvents i.e. by non-solvent process and more particularly, to processes for producing .gamma.-cyclodextrin, which comprise: (1) passing a sugar solution containing cyclodextrins and reducing sugars as primary ingredients through a column packed with alkali or alkali earth metal salts of strongly acidic cation exchange resin to separate cyclodextrins fraction from reducing sugars fraction and then, passing the cyclodextrins fraction through a column packed with gel resin particles to separate and collect .gamma.-cyclodextrin and, (2) bringing the sugar solution into contact with a hydrophobic, synthetic adsorption resin comprising a porous polymer to adsorb only .gamma.-cyclodextrin or .gamma.- and .beta.-cyclodextrins and then, eluting the adsorbed cyclodextrin(s) with water to separate and collect .gamma.-cyclodextrin.
Type:
Grant
Filed:
July 27, 1981
Date of Patent:
November 29, 1983
Assignees:
Nihon Shokuhin Kako Co., Ltd., Rikagaku Kenkyusho
Abstract: Palatinose is produced from sucrose with an immobilized bacteria containing alpha-glucosyl transferase. The bacteria is immobilized by entrapping cells of the bacteria in calcium alginate gel granules and treating the granules with polyethyleneimine and glutaraldehyde. Palatinose is efficiently produced by packing the bacteria-containing granules in a column and passing a sucrose solution through the column at high velocity.
Abstract: This invention relates to a process for producing cyclodextrins, which comprises passing a solution containing cyclodextrins and reducing sugars as primary ingredients through a column packed with alkali or alkali earth metal salts of strongly acidic cation exchange resin to separate and collect cyclodextrins from the sugar solution.
Type:
Grant
Filed:
July 20, 1981
Date of Patent:
May 24, 1983
Assignees:
Nihon Shokuhin Kako Co., Ltd., Rikagaku Kenkyusho
Abstract: New indoxylmaltodextrins, an enzymatic process for their preparation and their use as a substrate for the analytical detection and the determination of amylases, and rapid diagnostic agents which contain such indoxylmaltodextrins are described.
Abstract: Isomaltulose is produced by a process in which at least the isomaltulose-forming enzyme system of an isomaltulose-forming micro-organism genus Erwinia is immobilized on calcium alginate gel and then the immobilized enzyme system is contacted with a sucrose solution to convert at least part of the sucrose to isomaltulose.
Type:
Grant
Filed:
October 28, 1980
Date of Patent:
November 16, 1982
Assignee:
Talres Development (N.A.) N.V.
Inventors:
Christopher Bucke, Peter S. J. Cheetham
Abstract: This invention relates to a 1-step process for the preparation of fructose polymers and ethyl alcohol from sucrose. The fructose polymers are especially useful for production of high fructose syrups.
Abstract: Certain glucans modify the biosynthetic route of extracellular polysaccharides involved in dental plaque development causing critical loss of adhesiveness. They interfere with the biosynthesis of cell-bound polysaccharides reducing and preventing agglutination of cells. They thus aid in the prevention of dental plaque formation or concomitant dental caries and periodontal disease.
Abstract: This invention relates to a 2-step process for the preparation of fructose polymers and ethyl alcohol from sucrose. The fructose polymers are especially useful for the production of high fructose syrups.
Abstract: A process for producing cyclodextrins which comprises using cultured medium of the strains belonging to genus Micrococcus, filtrate thereof or enzyme preparation obtained therefrom, especially, the process in which the ratio of .alpha.-cyclodextrin to .beta.-cyclodextrin can be desirably varied by regulating the condition of the reaction mixture, is disclosed.
Abstract: A maltodextrin phosphorylase limit dextrin in the presence of maltodextrin phosphorylase and inorganic phosphate, is used as a substrate for alpha-amylase, which initiates a series of enzymatic reactions resulting in a chromogen response which can be used to measure the concentration of alpha-amylase in a body fluid. A novel limit dextrin and its preparation also are described.
Type:
Grant
Filed:
July 1, 1980
Date of Patent:
December 8, 1981
Assignee:
Worthington Biochemical Corporation
Inventors:
Paul T. Nix, Rebecca D. Goldfarb, Linda J. Stong, Lorraine E. Sulick, Ramesh C. Trivedi, Stanley W. Morgenstern
Abstract: This invention relates generally to (1) processes for the production and isolation of a novel fructosyl transferase enzyme from the fermentation broth of Pullularia pullulans, (2) enzymatic transfructosylation of sucrose to produce a novel fructose-polymer containing substrate, and (3) production of fructose syrups containing greater than 55% fructose from said novel substrate.
Abstract: The present invention relates to processes for producing syrups or syrup solids containing fructose-terminated oligosaccharides, characterized by subjecting a mixture containing liquefied starch and either fructose or sucrose to the action of immobilized cyclodextrin glucanotransferase E.C. 2.4.1.19.
Abstract: A sweetener containing alpha-glycosyl stevioside which is obtained by allowing alpha-glucosyltransferase to react on an aqueous solution containing stevioside and alpha-glucosyl sugar compound.