Produced By The Action Of A Glycosyl Transferase (e.g., Alpha, Beta, Gamma-cyclodextrins By The Action Of Glycosyl Transferase On Starch, Etc.) Patents (Class 435/97)
  • Patent number: 4590160
    Abstract: A process for producing a stevioside derivative, which comprises reacting stevioside with a .beta.-1,3- or .beta.-1,4-glycosyl sugar compound in aqueous solution or aqueous suspension in the presence of a microorganism or enzyme having .beta.-1,3- or .beta.1,4-glycosyl transferring activity thereby to form .beta.-1,3- or .beta.-1,4-glycosyl stevioside.
    Type: Grant
    Filed: February 25, 1983
    Date of Patent: May 20, 1986
    Assignees: Dainippon Ink and Chemicals, Inc., Dic Fine Chemicals, Inc.
    Inventors: Hideji Nishihashi, Tadao Matsubayashi, Tadashi Katabami, Ken-ichi Matsuda
  • Patent number: 4537763
    Abstract: New .alpha.-glycosyl glycyrrhizins bearing two or more .alpha.-glucose residues are prepared. Such .alpha.-glycosylation is carried out by subjecting an aqueous solution of glycyrrhizin (or a salt thereof) and an amylaceous substance (e.g. starch or cyclodextrin) to the enzymatic action of an .alpha.-glycosyl transferase (e.g. cyclodextrin glucanotrasferase). The .alpha.-glycosyl glycyrrhizins are low-caloric, low-cariogenic, mild, non-bitter, non-lingering sweeteners which may be advantageously incorporated into foods, beverages, cosmetics, dentifrices and drugs.
    Type: Grant
    Filed: June 11, 1982
    Date of Patent: August 27, 1985
    Assignee: Kabushiki Kaisha Hayashibara Seibutsu Kagaku Kenkyujo
    Inventors: Toshio Miyake, Hiromi Hijiya
  • Patent number: 4505756
    Abstract: A maltodextrin phosphorylase limit dextrin in the presence of maltodextrin phosphorylase and inorganic phosphate, is used as a substrate for alpha-amylase, which initiates a series of enzymatic reactions resulting in a chromogen response which can be used to measure the concentration of alpha-amylase in a body fluid. A novel limit dextrin and its preparation also are described.
    Type: Grant
    Filed: September 1, 1981
    Date of Patent: March 19, 1985
    Assignee: CooperBiomedical, Inc.
    Inventors: Paul T. Nix, Rebecca D. Goldfarb, Linda J. Stong, Lorraine E. Sulick, Ramesh C. Trivedi, Stanley W. Morgenstern
  • Patent number: 4477568
    Abstract: A process for producing cyclodextrin from starch in which an aqueous solun of starch is subjected to the action of an active cyclodextrin glycosyltransferase and the reaction mixture containing cyclodextrin, starch degradation products and active enzyme is continuously subjected to an ultrafiltration process to effect passage of the formed cyclodextrin through the membrane, while retaining substantially all of the other starch degradation products and active enzyme, thus permitting more cyclodextrin to be formed in the retentate, which will then pass the membrane, collecting the aqueous solution of cyclodextrin and recovering the cyclodextrin.
    Type: Grant
    Filed: September 20, 1982
    Date of Patent: October 16, 1984
    Assignee: Proefstation voor Aardappelverweking-TNO en Cooperatieve Verkoop en Produktievereniging van Aardappelmeel en Derivaten AVERE B.A.
    Inventors: Hendrik Hokse, Frederik S. Kaper, Jacob T. Wijpkema
  • Patent number: 4454161
    Abstract: The present invention relates to a process for the production of branching enzyme, and a method for improving the qualities of food products therewith. According to the present invention, a large amount of branching enzyme with a high specific activity can be easily obtained, and employment of the branching enzyme in food processing remarkably improves the qualities of food products without changing the desirable inherent properties of their amylaceous constituent(s); thus, said food products retain their qualities over a long period of time, prolonging extremely their shelf lives.
    Type: Grant
    Filed: January 4, 1982
    Date of Patent: June 12, 1984
    Assignee: Kabushiki Kaisha Hayashibara Seibutsu Kagaku Kenkyujo
    Inventors: Shigetaka Okada, Sumio Kitahata, Shigeharu Yoshikawa, Toshiyuki Sugimoto, Kaname Sugimoto
  • Patent number: 4443538
    Abstract: Enzyme-containing cells immobilized in an alginate gel are stabilized by contacting the gel with glycerol in a ratio of cellstoglycerol of 2:1 to 1:5. The enzyme-containing cells preferably convert sucrose to isomaltulose.
    Type: Grant
    Filed: May 5, 1982
    Date of Patent: April 17, 1984
    Assignee: Tate & Lyle Public Limited Company
    Inventor: Peter S. J. Cheetham
  • Patent number: 4430322
    Abstract: Certain glucans modify the biosynthetic route of extracellular polysaccharides involved in dental plaque development causing critical loss of adhesiveness. They interfere with the biosynthesis of cell-bound polysaccharides reducing and preventing agglutination of cells. They thus aid in the prevention of dental plaque formation or concomitant dental caries and periodontal disease.
    Type: Grant
    Filed: April 28, 1982
    Date of Patent: February 7, 1984
    Assignee: Merck & Co., Inc.
    Inventors: Thomas H. Stoudt, Karl H. Nollstadt
  • Patent number: 4418144
    Abstract: This invention relates to a process for producing .gamma.-cyclodextrin without using any organic solvents i.e. by non-solvent process and more particularly, to processes for producing .gamma.-cyclodextrin, which comprise: (1) passing a sugar solution containing cyclodextrins and reducing sugars as primary ingredients through a column packed with alkali or alkali earth metal salts of strongly acidic cation exchange resin to separate cyclodextrins fraction from reducing sugars fraction and then, passing the cyclodextrins fraction through a column packed with gel resin particles to separate and collect .gamma.-cyclodextrin and, (2) bringing the sugar solution into contact with a hydrophobic, synthetic adsorption resin comprising a porous polymer to adsorb only .gamma.-cyclodextrin or .gamma.- and .beta.-cyclodextrins and then, eluting the adsorbed cyclodextrin(s) with water to separate and collect .gamma.-cyclodextrin.
    Type: Grant
    Filed: July 27, 1981
    Date of Patent: November 29, 1983
    Assignees: Nihon Shokuhin Kako Co., Ltd., Rikagaku Kenkyusho
    Inventors: Minoru Okada, Masamitsu Matsuzawa, Osamu Uezima, Teruo Nakakuki, Koki Horikoshi
  • Patent number: 4386158
    Abstract: Palatinose is produced from sucrose with an immobilized bacteria containing alpha-glucosyl transferase. The bacteria is immobilized by entrapping cells of the bacteria in calcium alginate gel granules and treating the granules with polyethyleneimine and glutaraldehyde. Palatinose is efficiently produced by packing the bacteria-containing granules in a column and passing a sucrose solution through the column at high velocity.
    Type: Grant
    Filed: August 12, 1981
    Date of Patent: May 31, 1983
    Assignee: Mitsui Sugar Co., Ltd.
    Inventors: Junichi Shimizu, Kazumasa Suzuki, Yoshikazu Nakajima
  • Patent number: 4384898
    Abstract: This invention relates to a process for producing cyclodextrins, which comprises passing a solution containing cyclodextrins and reducing sugars as primary ingredients through a column packed with alkali or alkali earth metal salts of strongly acidic cation exchange resin to separate and collect cyclodextrins from the sugar solution.
    Type: Grant
    Filed: July 20, 1981
    Date of Patent: May 24, 1983
    Assignees: Nihon Shokuhin Kako Co., Ltd., Rikagaku Kenkyusho
    Inventors: Minoru Okada, Masamitsu Matsuzawa, Osamu Uezima
  • Patent number: 4376197
    Abstract: New indoxylmaltodextrins, an enzymatic process for their preparation and their use as a substrate for the analytical detection and the determination of amylases, and rapid diagnostic agents which contain such indoxylmaltodextrins are described.
    Type: Grant
    Filed: January 2, 1981
    Date of Patent: March 8, 1983
    Assignee: Behringwerke Aktiengesellschaft
    Inventor: Kurt Wallenfels
  • Patent number: 4359531
    Abstract: Isomaltulose is produced by a process in which at least the isomaltulose-forming enzyme system of an isomaltulose-forming micro-organism genus Erwinia is immobilized on calcium alginate gel and then the immobilized enzyme system is contacted with a sucrose solution to convert at least part of the sucrose to isomaltulose.
    Type: Grant
    Filed: October 28, 1980
    Date of Patent: November 16, 1982
    Assignee: Talres Development (N.A.) N.V.
    Inventors: Christopher Bucke, Peter S. J. Cheetham
  • Patent number: 4356262
    Abstract: This invention relates to a 1-step process for the preparation of fructose polymers and ethyl alcohol from sucrose. The fructose polymers are especially useful for production of high fructose syrups.
    Type: Grant
    Filed: June 3, 1980
    Date of Patent: October 26, 1982
    Assignee: CPC International Inc.
    Inventor: Robert E. Heady
  • Patent number: 4340673
    Abstract: Certain glucans modify the biosynthetic route of extracellular polysaccharides involved in dental plaque development causing critical loss of adhesiveness. They interfere with the biosynthesis of cell-bound polysaccharides reducing and preventing agglutination of cells. They thus aid in the prevention of dental plaque formation or concomitant dental caries and periodontal disease.
    Type: Grant
    Filed: November 7, 1980
    Date of Patent: July 20, 1982
    Assignee: Merck & Co., Inc.
    Inventors: Thomas H. Stoudt, Karl H. Nollstadt
  • Patent number: 4335207
    Abstract: This invention relates to a 2-step process for the preparation of fructose polymers and ethyl alcohol from sucrose. The fructose polymers are especially useful for the production of high fructose syrups.
    Type: Grant
    Filed: June 3, 1980
    Date of Patent: June 15, 1982
    Assignee: CPC International Inc.
    Inventor: Robert E. Heady
  • Patent number: 4317880
    Abstract: This invention relates to a process especially useful for the preparation of novel fructose polymers and high fructose syrups from sucrose.
    Type: Grant
    Filed: June 3, 1980
    Date of Patent: March 2, 1982
    Assignee: CPC International Inc.
    Inventor: Robert E. Heady
  • Patent number: 4317881
    Abstract: A process for producing cyclodextrins which comprises using cultured medium of the strains belonging to genus Micrococcus, filtrate thereof or enzyme preparation obtained therefrom, especially, the process in which the ratio of .alpha.-cyclodextrin to .beta.-cyclodextrin can be desirably varied by regulating the condition of the reaction mixture, is disclosed.
    Type: Grant
    Filed: March 26, 1980
    Date of Patent: March 2, 1982
    Assignee: Sanraku-Ocean Co., Ltd.
    Inventors: Yoshiaki Yagi, Kageaki Kouno, Taiji Inui
  • Patent number: 4304854
    Abstract: A maltodextrin phosphorylase limit dextrin in the presence of maltodextrin phosphorylase and inorganic phosphate, is used as a substrate for alpha-amylase, which initiates a series of enzymatic reactions resulting in a chromogen response which can be used to measure the concentration of alpha-amylase in a body fluid. A novel limit dextrin and its preparation also are described.
    Type: Grant
    Filed: July 1, 1980
    Date of Patent: December 8, 1981
    Assignee: Worthington Biochemical Corporation
    Inventors: Paul T. Nix, Rebecca D. Goldfarb, Linda J. Stong, Lorraine E. Sulick, Ramesh C. Trivedi, Stanley W. Morgenstern
  • Patent number: 4276379
    Abstract: This invention relates generally to (1) processes for the production and isolation of a novel fructosyl transferase enzyme from the fermentation broth of Pullularia pullulans, (2) enzymatic transfructosylation of sucrose to produce a novel fructose-polymer containing substrate, and (3) production of fructose syrups containing greater than 55% fructose from said novel substrate.
    Type: Grant
    Filed: June 9, 1978
    Date of Patent: June 30, 1981
    Assignee: CPC International Inc.
    Inventor: Robert E. Heady
  • Patent number: 4254227
    Abstract: The present invention relates to processes for producing syrups or syrup solids containing fructose-terminated oligosaccharides, characterized by subjecting a mixture containing liquefied starch and either fructose or sucrose to the action of immobilized cyclodextrin glucanotransferase E.C. 2.4.1.19.
    Type: Grant
    Filed: March 8, 1979
    Date of Patent: March 3, 1981
    Assignee: Kabushiki Kaisha Hayashibara Seibutsu Kagaku Kenkyujo
    Inventors: Shigetaka Okada, Sumio Kitahata, Shigeharu Yoshikawa, Kentaro Miyake
  • Patent number: 4219571
    Abstract: A sweetener containing alpha-glycosyl stevioside which is obtained by allowing alpha-glucosyltransferase to react on an aqueous solution containing stevioside and alpha-glucosyl sugar compound.
    Type: Grant
    Filed: June 15, 1978
    Date of Patent: August 26, 1980
    Assignee: Kabushiki Kaisha Hayashibara Seibutsu Kagaku Kenkyujo
    Inventor: Toshio Miyake