Produced By The Action Of A Glycosyl Transferase (e.g., Alpha, Beta, Gamma-cyclodextrins By The Action Of Glycosyl Transferase On Starch, Etc.) Patents (Class 435/97)
-
Patent number: 6846656Abstract: The present invention provides a process for economically producing N-acetylneuraminic acid without using expensive materials such as pyruvic acid and phosphoenolpyruvic acid. The process comprises: allowing (i) a culture of a microorganism having N-acetylneuraminic acid aldolase activity or N-acetylneuraminic acid synthetase activity, or a treated matter of the culture, (ii) a culture of a microorganism capable of producing pyruvic acid or a treated matter of the culture, or a culture of a microorganism capable of producing phosphoenolpyruvic acid or a treated matter of the culture, (iii) N-acetylmannosamine, and (iv) an energy source which is necessary for the formation of pyruvic acid or phosphoenolpyruvic acid to be present in an aqueous medium to form and accumulate N-acetylneuraminic acid in the aqueous medium; and recovering N-acetylneuraminic acid from the aqueous medium.Type: GrantFiled: August 25, 2000Date of Patent: January 25, 2005Assignee: Kyowa Hakko Kogyo Co., Ltd.Inventors: Satoshi Koizumi, Kazuhiko Tabata, Tetsuo Endo, Akio Ozaki
-
Patent number: 6828128Abstract: The present invention provides a protein having a &bgr;1,3-galactosyltransferase activity derived from a microorganism; a DNA encoding the protein; a recombinant DNA containing the DNA and a vector; a transformant obtained by introducing the recombinant DNA into a host cell; a method for producing a protein having a &bgr;1,3-galactosyltransferase activity using the transformant; and a method for producing a galactose-containing carbohydrate using the transformant.Type: GrantFiled: July 9, 2001Date of Patent: December 7, 2004Assignee: Kyowa Hakko Kogyo Co., Ltd.Inventors: Katsuhide Miyake, Masaki Watanabe, Shinji Iijima
-
Patent number: 6825013Abstract: The invention relates to a recombinant DNA molecule which comprises genes for biosynthesizing acarbose and homologous pseudo-oligosaccharides; to oligonucleotide primers for the PCR amplification of the molecule; to proteins which can be obtained by expressing the genes located on a molecule; to vectors and host cells which comprise the above-mentioned DNA molecule; to proteins which are encoded by the DNA molecule; to proteins which are expressed by means of said vectors in said host cells; to processes for preparing acarbose by introducing the characterized genes into appropriate host organisms and/or eliminating these genes from the host organisms; to processes for completing the gene cluster of genes for biosynthesizing acarbose, to processes for isolating analogous gene clusters in organisms other than Streptomyces glaucescens GLA.Type: GrantFiled: August 7, 2001Date of Patent: November 30, 2004Assignee: Avantis Pharma Deutschland GmbHInventor: Heinrich Decker
-
Patent number: 6780624Abstract: The present invention is directed to nucleic acids encoding glycosyltransferases, the proteins encoded thereby, and to methods for synthesizing oligosaccharides using the glycosyltransferases of the invention. In particular, the present application is directed to identification a glycosyltransferase locus of Neisseria gonorrhoeae containing five open reading frames for five different glycosyltransferases. The functionally active glycosyltransferases of the invention are characterized by catalyzing reactions such as adding Gal &bgr;1→4 to GlcNAc or Glc; adding GalNAc or GlcNAc &bgr;1→3 to Gal; and adding Gal &agr;1→4 to Gal.Type: GrantFiled: December 3, 2001Date of Patent: August 24, 2004Assignee: The Rockefeller UniversityInventor: Emil C. Gotschlich
-
Patent number: 6777215Abstract: The present invention relates to variants of cyclomaltodextrin glucanotransferase of increased product specificity.Type: GrantFiled: March 22, 2001Date of Patent: August 17, 2004Assignee: Novozymes A/SInventors: Carsten Andersen, Bjarne Rønfeldt Nielsen, Lubbert Dijkhuizen, Bauke Dijkstra
-
Patent number: 6743606Abstract: The invention relates to a protein which presents identical or different catalytically active domains of glycosyltransferases and has a processive action. In particular, the same protein is successively active in at least two successive process steps.Type: GrantFiled: September 22, 2000Date of Patent: June 1, 2004Assignees: Gesellschaft Für Erwerb und Verwertung von Schutzrechten-GVS mbH, Forschungszentrum BorstelInventors: Frank P. Wolter, Petra Jorasch, Ernst Heinz, Ulrich Zähringer
-
Patent number: 6740509Abstract: Methods for the production of a mucin-type glycopeptide comprising a transglycosylation using a sugar acceptor such as peptide and a sugar donor as an oligosaccharide with an endo-&agr;-N-acetylgalactosaminidase under a given condition is disclosed. The method provides a new practical way to produce mucin-type glycopeptides in industry and can provide an sufficient amount of the mucin-type glycopeptides in a practical use.Type: GrantFiled: May 22, 2002Date of Patent: May 25, 2004Assignee: Ikuko Ishii KarakasaInventors: Ikuko Ishii Karakasa, Katsumi Ajisaka
-
Patent number: 6730502Abstract: The present invention describes two novel proteins having fructosyltransferase activity. Both enzymes are derived from lactobacilli, which are food-grade micro-organisms with the Generally Recognized As Safe (GRAS) status. One of these proteins produces an inulin and fructo-oligosaccharides, while the other produces a levan and fructo-oligosaccharides. According to the invention lactobacilli capable of producing an inulin and/or a levan and/or fructo-oligosaccharides using one or both of the fructosyltransferases can be used as a probiotic or a symbiotic.Type: GrantFiled: November 29, 2001Date of Patent: May 4, 2004Assignee: Nederlandse Organisatie voor Toegepast - Natuurwetenschappelijk Onderzoek TNOInventors: Sacha Adrianus Fokke Taco Van Hijum, Gerritdina Hendrika Van Geel-Schutten, Lubbert Dijkhuizen, Hakim Rahaoui
-
Patent number: 6709834Abstract: The structure and specificity of a recombinant &agr;2,3-sialyltransferase from Campylobacter spp., is disclosed. Also provided are methods for using the &agr;2,3-sialyltransferase in the production of desired carbohydrate structures and nucleic acids that encode the sialyltransferase.Type: GrantFiled: January 29, 2002Date of Patent: March 23, 2004Assignee: National Research Council of CanadaInventors: Michel Gilbert, Warren W. Wakarchuk
-
Patent number: 6703213Abstract: General methods for monitoring the activity of MurG, a GlcNAc transferase involved in bacterial cell wall biosynthesis, is disclosed. More particularly, the synthesis of simplified substrate analogs of Lipid I (the natural substrate for MurG), which function as acceptors for UDP-GlcNAc in an enzymatic reaction catalyzed by MurG, is described. Assays using the substrate analogs of the invention are further disclosed, which are useful for identifying a variety of other substrates, including inhibitors of MurG activity, for facilitating mechanistic and/or structural studies of the enzyme and for other uses. High throughput assays are also described.Type: GrantFiled: April 22, 2002Date of Patent: March 9, 2004Assignee: The Trustees of Princeton UniversityInventors: Suzanne Walker Kahne, Hongbin Men, Peter Park, Min Ge
-
Patent number: 6685949Abstract: A conjugate vaccine for Moraxella (Branhamella) catarrhalis comprising isolated lipooligosaccharide from which esterified fatty acids have been removed, to produce a detoxified lipooligosaccharide (dLOS), or from which lipid A has been removed, to produce a detoxified oligosaccharide (OS), which is linked to an immunogenic carrier. The vaccine is useful for preventing otitis media and respiratory infections caused by M. catarrhalis in mammals, including humans.Type: GrantFiled: July 5, 2000Date of Patent: February 3, 2004Assignee: The United States of America as represented by the Department of Health & Human ServicesInventors: Xin-Xing Gu, John B. Robbins
-
Patent number: 6677142Abstract: The invention relates to a method for producing polysaccharides containing &agr;-1,4-glucan chains. According to the inventive method, a glucosyl group acceptor undergoes a chain prolongation reaction by reacting it with saccharose in the presence of an amylosaccharase. The amount of the glucosyl group acceptor in the reaction mixture is chosen in such a way that the mole ratio of the available ends of the glucosyl group acceptor to the saccharose is at least 1:1,000 and/or the weight ratio of the glucosyl group acceptor to the saccharose is at least 1:0.Type: GrantFiled: August 22, 2001Date of Patent: January 13, 2004Assignee: Celanese Ventures GmbHInventors: Max Weissmüller, Martin Quanz, Nicholas Provart
-
Patent number: 6660502Abstract: The present invention relates to a process of preparing commercial quantities of glucose and/or fructose from sucrose, a process of preparing commercial quantities of glucose and a polyfructan from sucrose, a reactor for practicing same, a process of preparing commercial quantities of fructose and a polyglucan from sucrose and a reactor for practicing same.Type: GrantFiled: June 5, 2001Date of Patent: December 9, 2003Assignee: Magnolia Nutritionals, L.L.C.Inventors: Steven J. Catani, Stephen A. Roth, Edward J. McGuire, Juan L. Navia
-
Publication number: 20030215927Abstract: This invention pertains to nucleic acid fragments encoding plant glucosyltransferases, heretofore undescribed, that exhibit catalytic activity with p-hydroxybenzoic acid (pHBA) as a substrate and only attach glucose to the aromatic carboxyl group of pHBA, to form the pHBA glucose ester. These enzymes have potential applications both in vitro and in vivo, and their primary amino acid sequences can be used to identify other proteins that have similar kinetic properties.Type: ApplicationFiled: February 6, 2003Publication date: November 20, 2003Inventors: Paul V. Viitanen, Knut Meyer, Drew E. Van Dyk
-
Publication number: 20030215928Abstract: The inventors have discovered some striking, and not previously predicted structural similarities and differences between the structure of Novamyl and the reported structures of CGTases, and based on this they have constructed variants of maltogenic alpha-amylase having CGTase activity and variants of CGTase having maltogenic alpha-amylase activity. Further, on the basis of sequence homology between Novamyl and CGTases, the inventors have constructed hybrid enzymes with one or more improvements to specific properties of the parent enzymes, using recombinant DNA methodology.Type: ApplicationFiled: May 29, 2003Publication date: November 20, 2003Applicant: Novozymes A/SInventors: Joel Robert Cherry, Allan Svendsen, Carsten Denmark Andersen, Lars Beier, Torben Peter Frandsen, Thomas Schafer
-
Publication number: 20030207406Abstract: The present invention relates to a method of transferring at least two saccharide units with a polyglycosyltransferase, a polyglycosyltransferase and a gene encoding such a polyglycosyltransferase.Type: ApplicationFiled: March 7, 2002Publication date: November 6, 2003Applicant: Neose Technologies, Inc.Inventors: Karl F. Johnson, Stephen Roth, Stephanie L. Buczala
-
Publication number: 20030170696Abstract: The invention relates to isolated CGTases und isolated nucleic acid sequences encoding the CGTases. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the nucleic acid sequences as well as methods for producing and using the CGTases, in particular to cleaning and detergent composition comprising such CGTases.Type: ApplicationFiled: January 14, 2003Publication date: September 11, 2003Inventors: Per Lina Jorgensen, Claus Crone Fuglsang
-
Publication number: 20030153056Abstract: The invention is based on the discovery that various proteins, such as Fringe, Brainiac and homologues and orthologues thereof, possess glycosyltransferase activity. Fringe and Brainiac have been found to possess glycosyltransferase activity in transfering sugar residues onto certain proteins, so affecting the binding of effector molecules to these proteins. This discovery allows the design of drug molecules that specifically target this interaction, and has implications for the treatment of various diseases.Type: ApplicationFiled: April 17, 2003Publication date: August 14, 2003Inventors: Stephen Cohen, Katja Bruckner, Henrik Clausen, Birgit Keck
-
Publication number: 20030148471Abstract: The present invention relates to a method of producing maltose syrup, wherein starch is treated with a hexosyltransferase (E.C. 2.4.1) and then a beta-amylase and/or a maltogenic amy-lase, or variant thereof. The invention also relates to an intermediate product suitable as staring material in maltose production processes.Type: ApplicationFiled: January 14, 2003Publication date: August 7, 2003Inventors: Barrie Edmund Norman, Hanne Vang Hendriksen
-
Gene coding for cyclodextrin glucanotransferase chiefly producing gamma-cyclodextrin and use thereof
Publication number: 20030114417Abstract: The invention provides a gene (isolated nucleic acid molecule) encoding the cyclodextrin glucanotransferase which produces a considerable amount of &ggr;-cyclodextrin (&ggr;-CD) from the substrates selected from among starch and starch decomposition products such as dextrin, amylopectin and amylose; recombinant plasmids comprising this gene; transformants transformed with the recombinant plasmid; methods of manufacturing the cyclodextrin glucanotransferase by employing these transformants to act upon the substrates selected from among starch and decomposition products thereof and causing the production of &ggr;-CD as a main product; and methods of manufacturing &ggr;-CD and CD-comprising compositions having a desired CD balance (&agr;-, &bgr;- and &ggr;-CD balance) employing this recombinant cyclodextrin glucanotransferase.Type: ApplicationFiled: July 3, 2002Publication date: June 19, 2003Inventors: Masayasu Takada, Takahiro Ide, Yoshinori Nakagawa, Takeshi Yamamoto, Mikio Yamamoto -
Patent number: 6569649Abstract: Processes for preparing oligosaccharides, polysaccharides, glycolipids, glycoproteins, and other saccharide compositions are provided which involve the enzyme facilitated transfer of a preselected saccharide unit from a donor moiety to an acceptor moiety. In accordance with a preferred embodiment, saccharide compositions having a plurality of saccharide units are prepared by appending the saccharide units in iterative fashion to acceptor moieties which are themselves saccharide compositions prepared in accordance with this invention.Type: GrantFiled: June 7, 1995Date of Patent: May 27, 2003Assignee: The Trustees of the University of PennsylvaniaInventor: Stephen Roth
-
Publication number: 20030068669Abstract: The present invention provides combinatorial methods for rapidly generating a diverse library of glycorandomized structures, comprising incubating one or more aglycons and a pool of NDP-sugars in the presence of a glycosyltransferase. The glycosyltransferase may be one that is associated with or involved in production of natural secondary metabolites, or one which is putatively associated with or involved in production of natural secondary metabolites. The glycosyltransferase may show significant flexibility with respect to its NDP-sugar donors and/or its aglycons. NDP-sugar donors may be commercially available, or may be produced by utilizing mutant or wild type nucleotidyltransferases significant flexibility with respect to their substrates.Type: ApplicationFiled: April 1, 2002Publication date: April 10, 2003Inventor: Jon S. Thorson
-
Patent number: 6544778Abstract: A method for preparing saccharide compositions is disclosed. The method is reiterative and comprises the following three steps. (i) A glycosyltransferase capable of transferring a preselected saccharide unit to an acceptor moiety is isolated by contacting the acceptor moiety with a mixture suspected of containing the glycosyltransferase under conditions effective to bind the acceptor moiety and the glycosyltransferase and thereby isolate the glycosyltransferase. The acceptor moiety is a protein, a glycoprotein, a lipid, a glycolipid, or a carbohydrate. (ii) The isolated glycosyltransferase is then used to catalyze the bond between the acceptor moiety and the preselected saccharide unit. (iii) Steps (i) and (ii) are repeated a plurality of times with the intermediate product obtained in the first iteration of the method being used as the acceptor moiety of the second iteration.Type: GrantFiled: December 3, 2001Date of Patent: April 8, 2003Assignee: The Trustees of the University of PennsylvaniaInventor: Stephen Roth
-
Publication number: 20030054499Abstract: The present invention relates to a novel microorganism and a method for producing fructooligosaccharides and neofructooligosaccharides.Type: ApplicationFiled: May 13, 2002Publication date: March 20, 2003Inventors: Joon Sang Han, Kang June Park, Dae Sub Shin, Jung Hoon Kim, Jin Chul Kim, Ki Chang Lee, Woon Hwa Lee, Seung Wook Kim, Seung Won Park
-
Patent number: 6528274Abstract: Disclosed are methods for the enzymatic synthesis of &agr;-sialylated oligosaccharide glycosides. Specifically, in the disclosed methods, &agr;2,3-sialyltransferase is used to transfer an analogue of sialic acid, employed as its CMP-nucleotide derivative, to the non-reducing sugar terminus of an oligosaccharide having a fucosyl group in the penultimate saccharide unit to the non-reducing sugar terminus. The analogue of sialic acid and the oligosacchairde employed in this method are selected to be compatible with the sialyltransferase employed.Type: GrantFiled: September 3, 1999Date of Patent: March 4, 2003Assignee: Synsorb Biotech, Inc.Inventors: Monica Marija Palcic, Keiko Sujino
-
Publication number: 20030007984Abstract: The invention relates to the use of modified starch obtainable by treating amylose containing starch in aqueous medium with an enzyme from the group of the &agr;-1,4-&agr;-1,4-glucosyl transferases (EC 2.4.1.25) or an enzyme the activity of which corresponds to that of enzymes from the group just mentioned, as an agent for forming a thermoreversible gel. The invention also relates to products in the form of a thermoreversible gel having as gel-forming substance a modified starch as defined. The invention further relates to the use of a modified starch as defined in the form of an aqueous solution.Type: ApplicationFiled: April 3, 2002Publication date: January 9, 2003Applicant: COOPERATIEVE VERKOOP-EN PRODUCTIEVERENIGING VAN AARDAPPELMEEL EN DERIVATEN AVEBE B.A.Inventors: Gerrit Jan Willem Euverink, Doede Jacob Binnema
-
Patent number: 6475991Abstract: The present invention embraces methods and compositions which employ antimicrobial compositions which are &agr;-alkylglucosides and esters thereof.Type: GrantFiled: November 23, 1998Date of Patent: November 5, 2002Assignee: UliceInventors: Emmanuel Boures, Arnaud Messager
-
Patent number: 6465220Abstract: The use of human polypeptide N-acetylgalactosaminytransferase T-4 (GalNAc-T4) to glycosylate substrates is disclosed. The action of GalNAc-T4 can complement the activities of GalNAc-T1, -T2 and -T3. The glycosylated substrates are useful in preparation of vaccines and anti-inflammatory agents. A method of producing the soluble form of the enzyme is also disclosed.Type: GrantFiled: December 21, 1998Date of Patent: October 15, 2002Assignee: GlycoZym ApSInventors: Frau Helle Hassan, Henrik Clausen, Eric Paul Bennett, Detlef Eisenkrätzer, Jochen Gätgens
-
Patent number: 6444447Abstract: The present invention relates to methodology for polymer grafting by a polysaccharide synthase and, more particularly, polymer grafting using the hyaluronate synthase from Pasteurella multocida. The present invention also relates to coatings for biomaterials wherein the coatings provide protective properties to the biomaterial and/or act as a bioadhesive. Such coatings could be applied to electrical devices, sensors, catheters and any device which may be contemplated for use within a mammal. The present invention further relates to drug delivery matrices which are biocompatible and may comprise combinations of a biomaterial or a bioadhesive and a medicament or a medicament-containing liposome. The biomaterial and/or bioadhesive is a hyaluronic acid polymer produced by a hyaluronate synthase from Pasteurella multocida.Type: GrantFiled: November 10, 1999Date of Patent: September 3, 2002Assignee: The Board of Regents of the University of OklahomaInventor: Paul L. DeAngelis
-
Patent number: 6440703Abstract: This invention provides methods for practical in vitro synthesis of gangliosides and other glycolipids. The synthetic methods typically involve enzymatic synthesis, or a combination of enzymatic and chemical synthesis. One or more of the enzymatic steps is preferably carried out in the presence of an organic solvent.Type: GrantFiled: August 22, 2001Date of Patent: August 27, 2002Assignee: Neose Technologies, Inc.Inventor: Shawn DeFrees
-
Patent number: 6423833Abstract: A process for preparing functional sugar polymers comprising transferring a monosaccharide or oligosaccharide to an acceptor, removing by-products, separating polymers which have not achieved the desired chain length and recycling these underdeveloped polymers, and an apparatus for producing same.Type: GrantFiled: May 4, 1999Date of Patent: July 23, 2002Inventors: Steven J. Catani, Kathleen S. Laurenzo, Juan L. Navia, Robert E. Walkup
-
Patent number: 6406894Abstract: A process for preparing polyvalent, physiologically degradable carbohydrate-containing polymers by enzymatic glycosylation reactions is described. The carbohydrate-containing polymers thus prepared may be used for preparing carbohydrate building blocks. The polyvalent carbohydrate-containing polymers of the invention cause no intolerance reactions in vivo, either in their intact form or in the form of degradation products. The carbohydrate side chain of the carbohydrate-containing polymer is assembled by enzymatic glycosylation reactions in homogeneous aqueous buffer systems directly on a biodegradable polymer. The yields of the glycosylation reaction are significantly improved over known processes, and are often quantitative. This also provides a significant increase in the loading densities. A process for preparing free oligosaccharides by means of the carbohydrate-containing polymers of the invention is also described.Type: GrantFiled: December 11, 1997Date of Patent: June 18, 2002Assignee: Glycorex ABInventors: Brigitte Hoersch, Michael Ahlers, Gerhard Kretzschmar, Eckart Bartnik, Dick Seiffge
-
Patent number: 6399337Abstract: A bacterial &agr;1,3-fucosyltransferase gene and deduced amino acid sequence is provided. The gene is useful for preparing &agr;1,3-fucosyltransferase polypeptide, and active fragment thereof, which can be used in the production of oligosaccharides such as Lewis X, Lewis Y, and siayl Lewis X, which are structurally similar to certain tumor-associated carbohydrate antigens found in mammals. These product glycoconjugates also have research and diagnostic utility in the development of assays to detect mammalian tumors. In addition the polypeptide of the invention can be used to develop diagnostic and research assays to determine the presence of H. pylori in human specimens.Type: GrantFiled: June 5, 1998Date of Patent: June 4, 2002Assignee: The Governors of the University of AlbertaInventors: Diane E. Taylor, Zhongming Ge
-
Patent number: 6399336Abstract: This invention provides methods for practical in vitro sialylation of glycoproteins, including recombinantly produced glycoproteins. The methods are useful for large-scale modification of sialylation patterns.Type: GrantFiled: January 15, 1998Date of Patent: June 4, 2002Assignee: Neose Technologies, Inc.Inventors: James C. Paulson, Robert J. Bayer, Eric Sjoberg
-
Publication number: 20020061550Abstract: A method for preparing saccharide compositions is disclosed. The method is reiterative and comprises the following three steps.Type: ApplicationFiled: December 3, 2001Publication date: May 23, 2002Applicant: The Trustees of the University of PennsylvaniaInventor: Stephen Roth
-
Patent number: 6391595Abstract: The invention provides a novel transferase that acts on a saccharide, as a substrate, composed of at least three sugar units wherein at least three glucose residues on the reducing end are linked &agr;-1,4 so as to transfer the &agr;-1,4 lingages to a &agr;-1,&agr;-1 linkages; a process for producing the transferase; a gene coding for the same; and a process for producing an oligosaccharide by using the same.Type: GrantFiled: April 26, 1999Date of Patent: May 21, 2002Assignee: Kirin Beer Kabushiki KaishaInventors: Masaru Kato, Yutaka Miura
-
Patent number: 6379933Abstract: The present invention relates to a method of transferring at least two saccharide units with a polyglycosyltransferase, a polyglycosyltransferase and a gene encoding such a polyglycosyltransferase.Type: GrantFiled: June 24, 1999Date of Patent: April 30, 2002Assignee: Neose Technologies, Inc.Inventors: Karl F. Johnson, Stephen Roth, Stephanie L. Buczala
-
Publication number: 20020045222Abstract: Processes for preparing oligosaccharides, polysaccharides, glycolipids, glycoproteins, and other saccharide compositions are provided which involve the enzyme facilitated transfer of a preselected saccharide unit from a donor moiety to an acceptor moiety. In accordance with a preferred embodiment, saccharide compositions having a plurality of saccharide units are prepared by appending the saccharide units in iterative fashion to acceptor moieties which are themselves saccharide compositions prepared in accordance with this invention.Type: ApplicationFiled: June 7, 1995Publication date: April 18, 2002Inventor: STEPHEN ROTH
-
Patent number: 6346394Abstract: Disclosed is a recombinant thermostable enzyme which has a molecular weight of about 54,000-64,000 daltons and a pI of about 5.6-6.6, and releases trehalose from non-reducing saccharides having a trehalose structure as an end unit and a degree of glucose polymerization of at least 3. The enzyme has a satisfactorily-high thermostability, i.e. it is not substantially inactivated even when incubated in an aqueous solution (pH 7.0) at 85° C. for 60 min, and this facilitates the production of trehalose on an industrial scale and in a satisfactorily-high yield.Type: GrantFiled: April 6, 1998Date of Patent: February 12, 2002Assignee: Kabushiki Kaisha Hayashibara Seibutsu Kagaku KenkyujoInventors: Hitoshi Mitsuzumi, Michio Kubota, Toshiyuki Sugimoto
-
Patent number: 6338955Abstract: The present invention relates to a novel enzyme having &bgr;1→4 N-acetylglucosaminyltransferase (GnT-IV) activity; a gene encoding the enzyme; a recombinant DNA comprising the gene; a host cell comprising the recombinant DNA; a method for producing an enzyme protein having GnT-IV activity comprising culturing the host cell in a medium; and a saccharide in which the sugar chain is modified using a GnT-IV. According to the present invention, a novel GnT-IV, a method for producing the enzyme and a gene coding for the enzyme are provided. With the GnT-IV of the present invention, it has become possible to produce a saccharide having a branching structure which could not be formed with conventional glycosyltransferases. Thus, the GnT-IV of the invention is useful not only for producing or improving glycoconjugate type pharmaceuticals, reagents and foods, but also for modifying the sugar chain structure of any biopolymer.Type: GrantFiled: August 12, 1998Date of Patent: January 15, 2002Assignee: Kirin Beer Kabushiki KaishaInventors: Suguru Oguri, Mari Minowa, Aruto Yoshida, Naoyuki Taniguchi, Makoto Takeuchi
-
Patent number: 6331418Abstract: A method for preparing saccharide compositions which is reiterative and includes the following three steps. (i) A glycosyltransferase capable of transferring a preselected saccharide unit to an acceptor moiety is isolated by contacting the acceptor moiety with a mixture suspected of containing the glycosyltransferase under conditions effective to bind the acceptor moiety and the glycosyltransferase and thereby isolate the glycosyltransferase. The acceptor moiety is a protein, a glycoprotein, a lipid, a glycolipid, or a carbohydrate. (ii) The isolated glycosyltransferase is then used to catalyze the bond between the acceptor moiety and the preselected saccharide unit. (iii) Steps (i) and (ii) are repeated a plurality of times with the intermediate product obtained in the first iteration of the method being used as the acceptor moiety of the second iteration.Type: GrantFiled: June 7, 1995Date of Patent: December 18, 2001Assignee: Neose Technologies, Inc.Inventor: Stephen Roth
-
Patent number: 6319695Abstract: This invention contemplates improved methods of enzymatic production of carbohydrates especially fucosylated carbohydrates. Improved syntheses of glycosyl 1- or 2-phosphates using both chemical and enzymatic means are also contemplated. The phosphorylated glycosides are then used to produce sugar nucleotides that are in turn used as donor sugars for glycosylation of acceptor carbohydrates. Especially preferred herein is the use of a disclosed method for fucosylation.Type: GrantFiled: October 14, 1992Date of Patent: November 20, 2001Assignee: The Scripps Research InsituteInventors: Chi-Huey Wong, Yoshitaka Ichikawa, Gwo-Jenn Shen, Kun-Chin Liu
-
Publication number: 20010029247Abstract: The present invention embraces methods and compositions which employ antimicrobial compositions which are &agr;-alkylglucosides and esters thereof.Type: ApplicationFiled: November 23, 1998Publication date: October 11, 2001Inventors: EMMANUEL BOURES, ARNAUD MESSAGER
-
Publication number: 20010024814Abstract: The present invention relates to a novel enzyme having &bgr;1→4 N-acetylglucosaminyltransferase (GnT-IV) activity; a gene encoding the enzyme; a recombinant DNA comprising the gene; a host cell comprising the recombinant DNA; a method for producing an enzyme protein having GnT-IV activity comprising culturing the host cell in a medium; and a saccharide in which the sugar chain is modified using a GnT-IV.Type: ApplicationFiled: August 12, 1998Publication date: September 27, 2001Inventors: SUGURU OGURI, MARI MINOWA, ARUTO YOSHIDA, NAOYUKI TANIGUCHI, MAKOTO TAKEUCHI
-
Patent number: 6270987Abstract: The present invention describes the identification, purification, recombinant production and characterization of novel O-fucosyltransferase enzymes.Type: GrantFiled: June 15, 1999Date of Patent: August 7, 2001Assignee: Genentech, Inc.Inventors: Yang Wang, Michael W. Spellman
-
Publication number: 20010007760Abstract: Disclosed are methods for the enzymatic synthesis of &agr;-sialylated oligosaccharide glycosides. Specifically, in the disclosed methods, &agr;2,3-sialyltransferase is used to transfer an analogue of sialic acid, employed as its CMP-nucleotide derivative, to the non-reducing sugar terminus of an oligosaccharide having a fucosyl group in the penultimate saccharide unit to the non-reducing sugar terminus. The analogue of sialic acid and the oligosacchairde employed in this method are selected to be compatible with the sialyltransferase employed.Type: ApplicationFiled: February 27, 2001Publication date: July 12, 2001Inventors: Monica Marija Palcic, Keiko Sujino
-
Patent number: 6248325Abstract: The present invention provides a human glutathione s-transferase (HGST) and polynucleotides which identify and encode HGST. The invention also provides genetically engineered expression vectors and host cells comprising the nucleic acid sequences encoding HGST and a method for producing HGST. The invention also provides for agonists, antibodies, or antagonists specifically binding HGST, and their use, in the prevention and treatment of cancer and other diseases associated with the expression of HGST. Additionally, the invention provides for the use of antisense molecules to polynucleotides encoding HGST for the treatment of cancer and other diseases associated with the expression of HGST. The invention also provides diagnostic assays which utilize the polynucleotide, or fragments or the complement thereof, and antibodies specifically binding HGST.Type: GrantFiled: May 11, 1999Date of Patent: June 19, 2001Assignee: Incyte GenomicsInventors: Surya K. Goli, Jennifer L. Hillman
-
Patent number: 6235510Abstract: ppGaNTase-T6 polypeptides and polynucleotides and methods for producing such polypeptides by recombinant techniques are disclosed. Also disclosed are methods for utilizing ppGaNTase-T6 polypeptides and polynucleotides in diagnostic assays.Type: GrantFiled: August 18, 1999Date of Patent: May 22, 2001Assignee: SmithKline Beecham CorporationInventors: Michael W Lark, Sanjay Kumar, Marion M Van Horn
-
Patent number: 6210933Abstract: The structure and specificity of the recombinant &agr;-2,3-sialyltransferases from Neisseria spp, are disclosed. Their use in the production of desired carbohydrate structures is also provided.Type: GrantFiled: September 1, 1999Date of Patent: April 3, 2001Assignee: National Research Council of CanadaInventors: Michel Gilbert, Warren W. Wakarchuk, N. Martin Young, Michael P. Jennings, Edward Richard Moxon
-
Patent number: 6207157Abstract: A conjugate vaccine for Nontypeable Haemophilus influenzae comprising lipooligosaccharide from which esterified fatty acids have been removed conjugated to an immunogenic carrier. The vaccine is useful for prevention of otitis media and respiratory infections in mammals.Type: GrantFiled: April 23, 1997Date of Patent: March 27, 2001Assignee: The United States of America as represented by the Department of Health and Human ServicesInventors: Xin-Xing Gu, Chao-Ming Tsai, David J. Lim, John B. Robbins