Produced By The Action Of A Carbohydrase (e.g., Maltose By The Action Of Alpha Amylase On Starch, Etc.) Patents (Class 435/99)
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Publication number: 20140096287Abstract: The present invention relates to isolated polypeptides having endoglucanase activity and isolated polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides.Type: ApplicationFiled: November 14, 2013Publication date: April 3, 2014Inventors: Kristian Krogh, Paul Harris
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Publication number: 20140087405Abstract: The object is to provide an ?-glucosidase in which a transglycosylation activity predominates, and use thereof, and the like. A modified ?-glucosidase consisting of an amino acid sequence in which one or two or more of the amino acid(s) is selected from a group of specific amino acids.Type: ApplicationFiled: March 5, 2012Publication date: March 27, 2014Applicant: AMANO ENZYME INC.Inventor: Satoru Ishihara
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Publication number: 20140087432Abstract: Methods for producing ethanol from cellulosic biomass and, in particular, methods for conditioning pretreated biomass are disclosed. In some embodiments, pretreated biomass is contacted with a cooling fluid in a flash vessel to cool the biomass. The amount of alkaline solution contacted with the biomass may be based on the pH of partially hydrolyzed pretreated biomass in a liquefaction bioreactor.Type: ApplicationFiled: September 24, 2012Publication date: March 27, 2014Applicant: ABENGOA BIOENERGYInventors: Quang A. Nguyen, Leroy D. Holmes, James F. Schelert, Mark A. Borchers
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Publication number: 20140087429Abstract: The present disclosure relates to an enzyme blend comprising a low pH, thermostable alpha-amylase and a Bacillus licheniformis alpha-amylase. The blend can include at least about 1.0 Liquefon Unit (LU) of the B. licheniformis alpha-amylase for every 5.0 Modified Wohlgemuth Unit (MWU) of the low pH, thermostable alpha-amylase. The enzyme blend described is suitable for starch liquefaction and saccharification, ethanol production, and/or sweetener production, among other things. Also provided herein is a method of processing a starch by liquefying the starch with the low pH, thermostable alpha-amylase and the Bacillus licheniformis alpha-amylase, simultaneously or sequentially.Type: ApplicationFiled: August 29, 2013Publication date: March 27, 2014Applicant: Danisco US Inc.Inventors: Glenn E. Nedwin, Vivek Sharma, Jayarama K. Shetty
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Publication number: 20140087433Abstract: Biomass (e.g., plant biomass, animal biomass, and municipal waste biomass) is processed to produce useful intermediates and products, such as energy, fuels, foods or materials. For example, systems are described that can use feedstock materials, such as cellulosic and/or lignocellulosic materials, to produce an intermediate or product, e.g., by fermentation.Type: ApplicationFiled: November 21, 2012Publication date: March 27, 2014Applicant: XYLECO, INC.Inventor: Xyleco, Inc.
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Publication number: 20140083329Abstract: In one or more embodiments, the present disclosure provides for a process for preparing a dispersion of starch particles in an aqueous liquid. In one or more embodiments, the process includes introducing a feed starch and the aqueous liquid into a rotor stator mixer, maintaining the feed starch and the aqueous liquid in the rotor stator mixer at a temperature ranging from a gelation temperature to less than a solubilization temperature, and shearing the feed starch into starch particles with the rotor stator mixer to form the dispersion of starch particles in the aqueous liquid. In one or more embodiments, the starch particles produced by this process have an average particle size diameter of no larger than 2 micrometers and the dispersion has 20 to 65 weight percent of the starch particles based on a total weight of the dispersion.Type: ApplicationFiled: November 26, 2013Publication date: March 27, 2014Applicant: DOW GLOBAL TECHNOLOGIES LLCInventors: Gregory Welsch, Brian Ninness, Michael Read, Timothy Young, Michal Matteucci, David Hammond, Liang Hong, Donald Ervick, JR.
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Patent number: 8679795Abstract: The present invention relates to polypeptides having xylanase activity and nucleic acid sequences encoding such polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the nucleic acid constructs as well as methods for producing and using the polypeptides. One specific application of the xylanase is the selective hydrolysis of pentose sugar components of hemicellulose-containing plant biomass. The nucleotide sequence may be used for the production of the xylanase or optimized mutants thereof.Type: GrantFiled: December 15, 2010Date of Patent: March 25, 2014Assignee: Sud-Chemie IP GmbH & Co. KGInventors: Andreas Kohl, Isabel Unterstrasser, Markus Rarbach, Andre Koltermann, Christoph Reisinger, Thomas Brück, Ulrich Kettling, Gudmundur O. Hreggvidsson, Olafur H. Fridjonsson
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Patent number: 8679794Abstract: Method for extracting oils, proteins and fermentable sugars from vegetable material in an aqueous medium, includes: a) adding water to the vegetable material; b) adding an enzyme mixture containing at least one cellulase, at least one hemicellulase, and at least one pectinase, the ratio between the pectinase activity and the cellulase activity being at least 0.14, and the ratio between the pectinase activity and the hemicellulase activity being at least 7.10?3, the pectinase activity being less than 120 ?mol/min/mL; c) incubating the vegetable material and the enzyme mixture with stirring to release oils, proteins and fermentable sugars in the reaction medium; d) separating the reaction medium to obtain free oil, an aqueous phase containing proteins and fermentable sugars, and a solid phase; e) optionally separating and recycling an emulsion of free oil or aqueous phase, to the medium; f) separating the proteins and fermentable sugars from the aqueous phase.Type: GrantFiled: October 14, 2010Date of Patent: March 25, 2014Assignee: Universite de LorraineInventors: Lionel Muniglia, Michel Girardin, Bernadette Piffaut, Guillaume Ricochon
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Publication number: 20140079683Abstract: A multifunctional polypeptide capable of hydrolyzing cellulosic materials, xylan, and mannan is disclosed. The polypeptide includes the catalytic core (cc) of Clostridium thermocellum Cthe_0797 (CelE), the cellulose-specific carbohydrate-binding module CBM3 of the cellulosome anchoring protein cohesion region (CipA) of Clostridium thermocellum (CBM3a), and a linker region interposed between the catalytic core and the cellulose-specific carbohydrate binding module. Methods of using the multifunctional polypeptide are also disclosed.Type: ApplicationFiled: September 18, 2013Publication date: March 20, 2014Applicant: Wisconsin Alumni Research FoundationInventors: Brian G. Fox, Taichi Takasuka, Christopher M. Bianchetti
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Publication number: 20140080182Abstract: The present invention relates to cellobiohydrolase variants and cellulose binding domain variants. The present invention also relates to polynucleotides encoding the variants; nucleic acid constructs, vectors, and host cells comprising the polynucleotides; and methods of using the variants.Type: ApplicationFiled: March 30, 2012Publication date: March 20, 2014Applicant: Novozymes, Inc.Inventors: Aubrey Jones, Paul Harris, Tia Heu, Doreen Bohan
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Publication number: 20140080178Abstract: The present invention relates to isolated polypeptides having cellulolytic enhancing activity and isolated polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides.Type: ApplicationFiled: March 9, 2012Publication date: March 20, 2014Inventors: Kirk Schnorr, Randall Kramer
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Publication number: 20140080183Abstract: This disclosure describes techniques for using a single feedstock of barley to produce a fermented product and a method for filtering a large-particles stream from a liquid stream containing small particles of a process stream using a series of mechanical separation devices to increase yield.Type: ApplicationFiled: September 16, 2013Publication date: March 20, 2014Applicant: ICM, Inc.Inventors: Kurt A Dieker, Scott D. Kohl, Justin McMillen, Keith Tjaden
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Publication number: 20140079931Abstract: The present invention relates to cellulose-based materials comprising nanofibrillated cellulose (NFC) from native cellulose. exhibiting highly superior properties as compared to other cellulose-based materials, a method for preparing such cellulose-based material, and uses thereof are also disclosed.Type: ApplicationFiled: March 26, 2012Publication date: March 20, 2014Applicant: CELLUTECH ABInventors: Lars Berglund, Houssine Sehaqui, Qi Zhou
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Patent number: 8673596Abstract: A process for production of fermentable sugars from biomass using multi-enzyme multi-step system is provided herein. The process disclosed in the present invention provides high yielded sugars in less time period. The multi-enzyme system disclosed in the present invention converts celluloses, hemicelluloses and/or mixture thereof to fermentable sugar with higher efficiency and better economics than the process known in the prior art. Cellulose and hemicelluloses fractions derived from natural sources such as any lignocellulosic biomass are saccharified in a shortened time with higher conversion rates of intermediates with modified enzymatic compositions/groups of the Multi-enzyme system to enhance the rate thus providing an economical cellulose and hemicellulose saccharification process.Type: GrantFiled: November 20, 2012Date of Patent: March 18, 2014Assignee: Chemical Engineering Department, Institute of Chemical Technology (Deemed University)Inventors: Arvind Mallinath Lali, Pooja Devidas Nagwekar, Jayesh Suman Varavedekar, Prathamesh Chandrashekhar Wadekar, Swapnali Subhash Gujarathi, Rajeshwar Dattatray Valte, Sachinkumar Hiraman Birhade, Annamma Anil Odaneth
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Publication number: 20140073017Abstract: The present invention relates to compositions that can be used in hydrolyzing biomass such as compositions comprising a polypeptide having ?-glucosidase activity, methods for hydrolyzing biomass material, and methods for improving the stability and saccharification efficacy of a composition comprising such ?-glucosidase polypeptides and/or activity.Type: ApplicationFiled: March 16, 2012Publication date: March 13, 2014Applicant: DANISCO US INC.Inventors: Thijs Kaper, Igor Nikolaev, Suzanne E. Lantz, Meredith K. Fujdala, Megan Y. Hsi
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Publication number: 20140073016Abstract: The present invention relates to a method for treating a lignocellulose biomass in order to dissolve the lignin therein, while the cellulose does not dissolve. The cellulose pulp obtained can be used to produce glucose. In addition the lignin can be isolated for subsequent use in the renewable chemical industry as a source for aromatic platform chemicals.Type: ApplicationFiled: December 15, 2011Publication date: March 13, 2014Applicant: IMPERIAL INNOVATIONS LTD.Inventors: Agnieszka Brandt, Richard J. Murphy, David J. Leak, Tom Welton, Jason Hallett
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Publication number: 20140075601Abstract: The present invention relates to alpha-amylases, nucleic acids encoding the alpha-amylases, methods of producing the alpha-amylases, and methods of using the alpha-amylases.Type: ApplicationFiled: October 28, 2013Publication date: March 13, 2014Applicant: NOVOZYMES A/SInventors: Carsten Andersen, Thomas Agersten Poulsen
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Publication number: 20140075603Abstract: The present invention relates to isolated polypeptides having cellulolytic enhancing activity and isolated polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides.Type: ApplicationFiled: November 14, 2013Publication date: March 13, 2014Inventors: Lan Tang, Ye Liu, Junxin Duan, Wenping Wu, Randall Kramer
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Publication number: 20140065677Abstract: The present invention relates to isolated polypeptides having cellulolytic activity or hemicellulolytic activity and polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides.Type: ApplicationFiled: January 26, 2012Publication date: March 6, 2014Applicant: Novozymes A/SInventors: Lene Lange, Peter K. Busk
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Publication number: 20140065671Abstract: The present invention relates to isolated polypeptides having cellobiohydrolase activity, catalytic domains, and cellulose binding domains and polynucleotides encoding the polypeptides, catalytic domains, and cellulose binding domains. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides, catalytic domains, or cellulose binding domains.Type: ApplicationFiled: January 26, 2012Publication date: March 6, 2014Applicants: Novozymes, Inc., Novozymes A/SInventors: Mary Ann Stringer, Brett McBrayer
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Patent number: 8663952Abstract: A method of producing corn starch by enzymatic process involving: soaking the corn; crushing the corn, separating and washing embryo; fine grinding; washing and drying fiber; separating and drying protein; washing, dewatering and drying the starch. An enzyme preparation is added before the step of washing, dewatering and drying the starch; the enzyme preparation is cellulose, or xylanase, or combination of the cellulose and the xylanase; and addition of the enzyme preparation is from 0.001% to 0.08% by weight of the corn. Based on the technology of traditional wet process, the method of the present invention comprises a step of adding enzyme preparation in the process of separating the corn, which improves the effect and the efficiency of mechanical separation, and further improves the purity and yield of the substance separated while also reducing the energy consumption.Type: GrantFiled: February 24, 2012Date of Patent: March 4, 2014Assignee: Baiyin Sino Biotechnology Co., Ltd.Inventors: Xinmin He, Feng Yu, Shuangjing Wang, Qiang Cui, Weiguo Sun
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Publication number: 20140057324Abstract: Variants of Bacillus sp. no. 707 alpha-amylase are provided that are produced more efficiently and thus more economically. Higher fermentation yields are achieved through introducing amino acid variations that promote solubility of the variant in a fermentation broth. Increased solubility allows more enzyme to remain in solution after expression in a host cell. This in turn increases the efficiency with which the expressed variant enzyme can be recovered from the fermentation broth.Type: ApplicationFiled: August 23, 2013Publication date: February 27, 2014Applicant: Danisco US Inc.Inventors: Wolfgang AEHLE, Neelam S. AMIN
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Publication number: 20140059719Abstract: The present invention relates to chimeric GH61 polypeptides having cellulolytic enhancing activity. The present invention also relates to polynucleotides encoding the chimeric GH61 polypeptides; nucleic acid constructs, vectors, and host cells comprising the polynucleotides; and methods of using the chimeric GH61 polypeptides.Type: ApplicationFiled: November 18, 2011Publication date: February 27, 2014Applicant: Novozymes, Inc.Inventors: Mark Wogulis, Matthew Sweeney, Tia Heu
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Patent number: 8658398Abstract: The present invention provides for heterologous expression of termite and termite-associated symbiont cellulases. The cellulases can, for example, be codon-optimized and expressed in yeast host cells, such as the yeast Saccharomyces cerevisiae. The cellulases can also be co-expressed in host cells with other cellulases. The expression in such host cells of the termite and termite-associated symbiont cellulases, and variants and combinations thereof, result in yeast with improved cellulosic activity. Thus, such genes and expression systems are useful for efficient and cost-effective consolidated bioprocessing systems.Type: GrantFiled: July 7, 2009Date of Patent: February 25, 2014Assignee: Mascoma CorporationInventors: Elena E. Brevnova, Vineet Rajgarhia, Mark Mellon, Anne Warner, John McBride, Chhayal Gandhi, Erin Wiswall
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Publication number: 20140051119Abstract: The present invention provides fungal xylanase and/or beta-xylosidase enzymes suitable for use in saccharification reactions. The present application further provides genetically modified fungal organisms that produce xylanase and/or beta-xylosidases, as well as enzyme mixtures exhibiting enhanced hydrolysis of cellulosic material to fermentable sugars, enzyme mixtures produced by the genetically modified fungal organisms, and methods for producing fermentable sugars from cellulose using such enzyme mixtures.Type: ApplicationFiled: June 10, 2013Publication date: February 20, 2014Applicant: Codexis, Inc.Inventors: Ryan Fong, Xiyun Zhang, Chirs Noriega, Nicholas Agard, Anupam Gohel, Derek Smith
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Publication number: 20140051129Abstract: The present disclosure provides methods of potentiating the activity of an enzyme cocktail by the addition of one or more enzymes. In some embodiments, a sub-maximum or sub-optimal dose of the cocktail may be used in combination with the enzymes. In some embodiments, the enzyme or enzymes are expressed in planta.Type: ApplicationFiled: February 22, 2012Publication date: February 20, 2014Applicant: SYNGENTA PARTICIPATIONS AGInventors: Jason Nichols, Brian Ember, Paul Oeller, Myoung Kim
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Publication number: 20140051130Abstract: The present invention relates to enzyme compositions for high temperature saccharification of cellulosic material and to uses thereof.Type: ApplicationFiled: October 11, 2013Publication date: February 20, 2014Inventors: Brett McBrayer, Tarana Shaghasi, Elena Vlasenko
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Publication number: 20140051128Abstract: The present invention relates to production of fermentable sugars from lignocellulosic material by enzymatic conversion. The fermentable sugars are useful e.g. in the production of bioethanol. Novel polypeptides having endoglucanase activity, polynucleotides encoding them and vectors and host cells containing the polynucleotides are disclosed. A method for treating cellulosic material with the novel endoglucanase as well as uses of the enzymes and enzyme preparations and a method of preparing them are described.Type: ApplicationFiled: May 24, 2013Publication date: February 20, 2014Applicant: ROAL OYInventors: Taija LEINONEN, Alexandra KOMANDER, Kim LANGFELDER, Jari VEHMAANPERÄ, Terhi PURANEN
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Publication number: 20140045225Abstract: The present invention discloses novel polypeptides and enzyme preparations containing them, which enhance the efficiency of the cellulosic degradation even at elevated temperatures. The polypeptides are produced by recombinant technology, and means for their production are described. The novel polypeptides are useful in processing biomass, and in biofuel, starch, textile, detergent, pulp and paper, food, feed or beverage industries. They may also be used e.g. in cleaning the interior of a dishwashing machine or for biofinishing or biostoning. The novel polypeptides are also useful in animal feed.Type: ApplicationFiled: June 6, 2013Publication date: February 13, 2014Applicant: Roal OyInventors: Susanna MAKINEN, Kari JUNTUNEN, Alexandra KOMANDER, Kim LANGFELDER, Jari VEHMAANPERA, Terhi PURANEN
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Publication number: 20140045224Abstract: A process for producing a saccharified solution in an improved yield when filtering the mixture obtained by saccharifying lignocellulosic biomass is provided. A mixture containing a saccharified solution and an undecomposed biomass residue by treating a substrate biomass using a saccharification enzyme is obtained. The mixture is filtered to obtain the saccharified solution as a filtrate. Before filtering the mixture, 95% by mass or more of the particles, based on the total amount of particles contained in a centrifuged supernatant of the mixture, is adjusted to have a particle size ranging from 1 to 30 ?m.Type: ApplicationFiled: May 28, 2013Publication date: February 13, 2014Applicant: HONDA MOTOR CO., LTD.Inventor: Hiroyuki TANAKA
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Publication number: 20140045227Abstract: The present invention provides a process for degradation of lignocellulosic biomass, which has optionally been pre-treated. It is based on the finding that hydrolysis efficiency of the biomass is enhanced in the presence of a mechanically or chemically treated microorganism capable of producing the respective hydrolytic enzymes. The invention therefore provides a process for degradation of lignocellulosic biomass with integrated produced enzyme cocktails. The invention also provides a process wherein the part of the optionally pre-treated lignocellulosic biomass is incorporated into the final growth medium of the fungus.Type: ApplicationFiled: January 2, 2012Publication date: February 13, 2014Applicant: CLARIANT PRODUKTE (DEUTSCHLAND) GMBHInventors: Markus Rarbach, Zdravko Dragovic, Andreas Kohl, Jochen Gerlach, Jörg Bartuch, Thomas Brück
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Publication number: 20140045226Abstract: A process for treating lignocellulosic biomass is described. The process includes treating particulate biomass with alkali to partially remove hemicellulose sheath on cellulose microfibrils of the biomass; and carrying out an oxidation reaction on the alkali treated biomass to form a treated biomass having a disrupted cellulose crystalline structure. The treated biomass may be further processed to form free sugars and lignin and the free sugars can be used to produce bio-ethanol or used as sugar source in fermentation processes.Type: ApplicationFiled: July 9, 2013Publication date: February 13, 2014Inventors: J. Bruce WICKING, Yilin BIAN, Ping LIU, Monlin Kuo
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Publication number: 20140038243Abstract: A method for treating biomass is provided, which includes, in a reactor, allowing gaseous ammonia to condense on the biomass and react with water present in the biomass to produce pretreated biomass, wherein reactivity of polysaccharides in the biomass is increased during subsequent biological conversion as compared to polysaccharides in biomass which has not been pretreated. A method for treating biomass with a liquid ammonia and recovering the liquid ammonia is also provided. Related systems which include a biochemical or biofuel production facility are disclosed.Type: ApplicationFiled: December 22, 2011Publication date: February 6, 2014Applicant: BOARD OF TRUSTEES OF MICHIGAN STATE UNIVERSITYInventors: Venkatesh Balan, Bruce E. Dale, Shishir Chundawat, Leonardo Sousa
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Publication number: 20140038244Abstract: Provided is a system for localizing and optimizing harvesting of woody biomass by providing a plurality of portable pretreatment units at harvesting or mill sites. The sugars derived from woody waste products can be transported to plants for processing or processing directly by plants located at mill sites to generate biofuels and other chemicals.Type: ApplicationFiled: October 9, 2013Publication date: February 6, 2014Applicant: Sweetwater Energy, Inc.Inventors: Arunas Chesonis, Jerry W. Horton
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Publication number: 20140030767Abstract: The present invention relates to variants (mutants) of parent Termamyl-like alpha-amylases, which variant has alpha-amylase activity and exhibits altered stability, in particular at high temperatures and/or at low pH relative, and/or low Ca2+ to the parent alpha-amylase.Type: ApplicationFiled: May 31, 2013Publication date: January 30, 2014Applicant: Novozymes A/SInventor: Novozymes A/S
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Publication number: 20140030770Abstract: The present invention relates to isolated polypeptides having cellobiohydrolase activity and polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides.Type: ApplicationFiled: January 26, 2012Publication date: January 30, 2014Applicant: NOVOZYMES INC.Inventor: Nikolaj Spodsberg
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Publication number: 20140030769Abstract: Disclosed herein are combinations of free fungal enzymes and cellulosomes useful for the hydrolysis of cellulose and the conversion of biomass. Methods of degrading cellulose and biomass using the combinations are also disclosed.Type: ApplicationFiled: July 29, 2013Publication date: January 30, 2014Applicant: Alliance for Sustainable Energy, LLCInventors: Michael RESCH, John O. BAKER, Xu QI, William S. ADNEY, Steven R. DECKER, Michael E. HIMMEL, Bryon DONOHOE
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Publication number: 20140030768Abstract: Biomass (e.g., plant biomass, animal biomass, and municipal waste biomass) is processed to produce useful products, such as fuels. For example, systems can use feedstock materials, such as cellulosic and/or lignocellulosic materials and/or starchy materials, to produce ethanol and/or butanol, e.g., by fermentation.Type: ApplicationFiled: June 20, 2013Publication date: January 30, 2014Inventor: Marshall Medoff
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Publication number: 20140026261Abstract: The present invention provides isolated polypeptides having cellulolytic enhancing activity and isolated polynucleotides encoding the polypeptides. The invention also provides nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides.Type: ApplicationFiled: April 25, 2012Publication date: January 23, 2014Inventors: Yu Zhang, Lan Tang, Svend Hostgaard Bang Henriksen
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Publication number: 20140024083Abstract: The invention relates to a variant of a parent Termamyl-like alpha-amylase, which variant has alpha-amylase activity and exhibits an alteration in at least one of the following properties relative to the parent alpha-amylase: substrate specificity, substrate binding, substrate cleavage pattern, thermal stability, pH/activity profile, pH/stability profile, stability towards oxidation, Ca2+ dependency and specific activity.Type: ApplicationFiled: July 29, 2013Publication date: January 23, 2014Inventors: Allan Svendsen, Torben Vedel Borchert, Henrik Bisgaard-Frantzen
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Publication number: 20140024084Abstract: This disclosure provides for materials and methods for converting biomass to biofuels. The materials include a colloid mill with or without cellulase enzymes, and the methods include the use of a colloid mill and optionally cellulose enzymes to pretreat biomass for use in a biomass to biofuel production process.Type: ApplicationFiled: September 27, 2013Publication date: January 23, 2014Applicant: EdeniQ, Inc.Inventors: Adriano Galvez, III, Glenn Richards
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Publication number: 20140024075Abstract: Provided herein are methods and compositions for bacterial cellulose production. In some embodiments, the methods and compositions involve or are made from distiller's grain.Type: ApplicationFiled: May 15, 2012Publication date: January 23, 2014Applicant: Shanghai Zhiyi Information Technology Ltd.Inventor: Feng Hong
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Publication number: 20140017737Abstract: The present invention relates to variants of a parent cellobiohydrolase II. The present invention also relates to polynucleotides encoding the variants; nucleic acid constructs, vectors, and host cells comprising the polynucleotides; and methods of using the variants.Type: ApplicationFiled: September 23, 2013Publication date: January 16, 2014Inventor: Mark Wogulis
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Publication number: 20140017733Abstract: The present invention provides fungal xylanase and/or xylosidase enzymes suitable for use in saccharification reactions. The present invention provides xylanase and xylosidase enzymes suitable for use in saccharification reactions. The present application further provides genetically modified fungal organisms that produce xylanase(s) and/or xylosidase(s), as well as enzyme mixtures exhibiting enhanced hydrolysis of cellulosic material to fermentable sugars, enzyme mixtures produced by the genetically modified fungal organisms, and methods for producing fermentable sugars from cellulose using such enzyme mixtures. In some embodiments, the xylanase and xylosidase enzyme(s) are M. thermophila enzymes.Type: ApplicationFiled: June 10, 2013Publication date: January 16, 2014Inventors: Nicholas John Agard, David Elgart, Jie Yang, Goutami Banerjee, Jeanne Bonomo Benoit, Dipnath Baidyaroy
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Publication number: 20140017736Abstract: A method of producing a sugar liquid includes hydrolyzing with a filamentous fungus-derived cellulase a product obtained by adding a water-soluble inorganic salt(s) to a pretreated product of cellulose to a final concentration of 5 to 35 g/L; and subjecting the hydrolysate to solid-liquid separation and filtering the obtained solution component through an ultrafiltration membrane to recover the filamentous fungus-derived cellulase as a non-permeate and to obtain a sugar liquid as a permeate.Type: ApplicationFiled: March 28, 2012Publication date: January 16, 2014Applicant: Toray Industries, Inc.Inventors: Hiroyuki Kurihara, Junpei Kishimoto, Katsushige Yamada
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Publication number: 20140017734Abstract: Nucleic acid molecules encoding chimeric cellulase polypeptides that exhibit improved cellulase activities are disclosed herein. The chimeric cellulase polypeptides encoded by these nucleic acids and methods to produce the cellulases are also described, along with methods of using chimeric cellulases for the conversion of cellulose to sugars such as glucose.Type: ApplicationFiled: July 12, 2013Publication date: January 16, 2014Inventors: Qi XU, John O. BAKER, Michael E. HIMMEL
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Publication number: 20140017732Abstract: The present application describes a dilute two-loop method for treating biomass in the production of biofuels such as ethanol. The method involves washing the biomass to dissolve sugars that are associated with the biomass, and separating the washed biomass into a solids phase and a liquids phase. The solids phase is pretreated to render the biomass more susceptible to hydrolysis under conditions that do not produce substantial amounts of sugars. The pretreated biomass is separated into a second solids phase and second liquid phase, and the second solids phase is saccharified and fermented. The first and second liquid phases are recycled to dilute the biomass at various stages of the process.Type: ApplicationFiled: March 15, 2013Publication date: January 16, 2014Applicant: Edeniq, Inc.Inventor: Edeniq, Inc.
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Publication number: 20140017735Abstract: Disclosed herein are enzymes and combinations of the enzymes useful for the hydrolysis of cellulose and the conversion of biomass. Methods of degrading cellulose and biomass using enzymes and cocktails of enzymes are also disclosed.Type: ApplicationFiled: July 15, 2013Publication date: January 16, 2014Inventors: Roman BRUNECKY, Michael E. HIMMEL
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Publication number: 20140011242Abstract: The present teachings provide methods of converting cellulosic materials to soluble sugars. Methods for increasing the yield of glucose from the enzymatic saccharification of cellulosic materials is also provided. The present teachings further provide methods of increasing the yield of cellobiose from the enzymatic saccharification of cellulosic materials.Type: ApplicationFiled: June 13, 2013Publication date: January 9, 2014Applicant: Danisco US Inc.Inventors: Bradley R. KELEMEN, Edmund A. LARENAS, Colin MITCHINSON
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Publication number: 20140004563Abstract: A system and method for the treatment of biomass comprising mixing a biomass with an ionic liquid (IL) to swell the biomass and electromagnetic (EM) heating, preferably radiofrequency (RF) heating, said biomass. Additionally, a method of acidolysis of biomass comprising mixing biomass in an ionic liquid (IL) to swell the biomass; adding an acid, to lower the pH of the biomass below pH 7; applying radio frequency (RF) heating to the biomass to heat to a target temperature range; applying ultrasonic heating, electromagnetic (EM) heating, convective heating, conductive heating, or combinations thereof, to the biomass to maintain the biomass at a target temperature range; washing the treated biomass; and recovering sugars and released lignin.Type: ApplicationFiled: June 21, 2013Publication date: January 2, 2014Applicant: SUGANIT SYSTEMS, INC.Inventors: Praveen PARIPATI, Anantharam DADI