Produced By The Action Of A Carbohydrase (e.g., Maltose By The Action Of Alpha Amylase On Starch, Etc.) Patents (Class 435/99)
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Publication number: 20140234906Abstract: The present invention relates to alpha-amylase variants. The present invention also relates to polynucleotides encoding the variants; nucleic acid constructs, vectors, and host cells comprising the polynucleotides; and methods of using the variants.Type: ApplicationFiled: October 17, 2012Publication date: August 21, 2014Inventors: Carsten Andersen, Signe Eskildsen Larsen, Esben Peter Friis, Peter Kamp Hansen, Henrik Friis Madsen, Anders Viksoe Nielsen, Pernille Ollendorf Micheelsen
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Publication number: 20140234913Abstract: In a process for hydrolyzing plant material in aqueous solution or suspension with an enzyme, the enzyme is delivered in solid form (e.g., as a spray-dried powder) in closed containers (such as paper bags or cardboard boxes), which are added directly in the process (i.e., addition of whole boxes/bags). The invention is particularly amenable to the production of first or second-generation bioethanol.Type: ApplicationFiled: January 30, 2014Publication date: August 21, 2014Applicant: Novozymes A/SInventor: Ole Simonsen
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Publication number: 20140234915Abstract: The present invention relates enzyme composition comprising a cellulolytic preparation and an acetylxylan esterase (AXE); and the used of cellulolytic enzyme compositions for hydrolyzing acetylated cellulosic material. Finally the invention also relates to processes of producing fermentation products from acetylated cellulosic materials using a cellulolytic enzyme composition of the invention.Type: ApplicationFiled: September 21, 2012Publication date: August 21, 2014Inventors: Prashant Iyer, Armindo Ribiero Gaspar, James Croonenberghs, Thomas P. Binder
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Patent number: 8809031Abstract: A method of making a Bacillus alpha-amylase variant that increases alpha-amylase production and the alpha-amylases produced thereby. The recombinant alpha-amylases can be placed in compositions and used for purposes of laundry detergents, cleaning and dishwashing detergents, fabric desizing, starch liquefaction, cereal liquefaction, starch saccharification, biofilm removal, and starch hydrolysis in cane sugar processing.Type: GrantFiled: March 20, 2008Date of Patent: August 19, 2014Assignee: Danisco US Inc.Inventors: George England, Marc Kolkman, Casper Vroemen
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Publication number: 20140230097Abstract: The present invention relates to isolated polypeptides having xylanase activity and polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides.Type: ApplicationFiled: June 24, 2013Publication date: August 14, 2014Inventor: Nikolaj Spodsberg
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Publication number: 20140227739Abstract: The present invention relates to isolated polypeptides having endoglucanase activity and polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides.Type: ApplicationFiled: February 12, 2013Publication date: August 14, 2014Inventor: Nikolaj Spodsberg
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Publication number: 20140230098Abstract: The present invention relates to isolated polypeptides having endoglucanase activity and polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides.Type: ApplicationFiled: June 27, 2013Publication date: August 14, 2014Inventor: Nikolaj Spodsberg
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Publication number: 20140230096Abstract: The present invention relates to isolated polypeptides having cellobiohydrolase activity and polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides.Type: ApplicationFiled: June 24, 2013Publication date: August 14, 2014Applicant: Novozymes Inc.Inventor: Nikolaj Spodsberg
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Publication number: 20140228314Abstract: Disclosed is a method for preparation of a chitosan oligosaccharide composition capable of increasing a content of disaccharide in chitosan oligosaccharide with excellent effects of reducing HbAlc and blood glucose as well as excellent thermal stability. The method for preparation of a chitosan oligosaccharide composition includes: adding chitosan to distilled water to swell the chitosan; firstly treating the chitosan with a chitosanase to prevent the chitosan solution from becoming a state of being viscose, then, adding an organic acid to a mixed solution of the chitosan and chitosanase at a predetermined time interval bit by bit over several additions; allowing mutual reaction between the chitosan, chitosanase and organic acid; and inactivating the chitosanase to obtain the chitosan oligosaccharide composition. Further, a medicine and/or health supplement food which is effective in preventing and treating diabetes, including at least 40% of disaccharide without monosaccharide, is also disclosed.Type: ApplicationFiled: November 14, 2013Publication date: August 14, 2014Applicant: KUNPOONG BIO CO., LTDInventors: Chen-Gum OH, Young-In KWON, Jong-Wook LEE, Seong-Chul KIM
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Publication number: 20140220641Abstract: Provided herein are methods and compositions for increasing the production of one or more cellulases from a fungal host cell. The disclosure is based, on the surprising discovery that mis-expression of the transcriptional regulator clr-2 in a filamentous fungal cell was able to induce expression of cellulase genes under non-inducing or starvation conditions, resulting in increased secretion of cellulases from the cell. Advantageously, mis-expression of the transcription factor clr-2 in a filamentous fungal cell cultured in the absence of cellulose or cellobiose results in increased secretion of cellulases.Type: ApplicationFiled: July 23, 2012Publication date: August 7, 2014Applicant: The Regents of the University of CaliforniaInventors: Chaoguang Tian, Teresa Shock, N. Louise Glass, Samuel Coradetti, James Craig
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Publication number: 20140220640Abstract: Disclosed is a method for detoxifying a lignocellulosic biomass hydrolysate, including: preparing a hydrolysate by pretreating a lignocellulosic biomass by hydrolysis; and decreasing or removing toxicity by adding a surfactant to the hydrolysate. The detoxifying method according to the present disclosure may effectively remove toxicity of compounds derived from lignin that inhibit the growth of and fermentation by microorganisms during the pretreatment of lignocellulosic biomass. Further, production efficiency can be improved since loss of sugar can be avoided during the detoxification and additional cost can be minimized.Type: ApplicationFiled: May 30, 2012Publication date: August 7, 2014Applicant: KOREA INSTITUTE OF SCIENCE AND TECHNOLOGYInventors: Young Soon Um, Kyung Min Lee, Ki Yeon Kim, Yun Je Kim, Byoung In Sang
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Publication number: 20140221643Abstract: Provided are a fructosylated mangiferin, a preparation method therefor and a use thereof, wherein the fructosylated mangiferin has a structural formula represented by the following formula (I), the method for preparing the fructosylated mangiferin includes adding a substance with fructosylating enzymatic activity to a transformed liquid containing mangiferin for biotransformation reaction, so as to convert the mangiferin into the fructosylated mangiferin, wherein the transformed liquid contains the mangiferin and a glycosyl donor; as well as a use of the fructosylated mangiferin in preparation of a medicament for treatment of tumor-related diseases.Type: ApplicationFiled: March 8, 2012Publication date: August 7, 2014Applicant: NANJING UNIVERSITY OF TECHNOLOGYInventors: Bingfang He, Xueming Wu, Jianlin Chu, Bin Wu, Sen Zhang, Pingkai Ouyang
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Publication number: 20140220635Abstract: The present invention also relates to polynucleotides encoding the variants; nucleic acid constructs, vectors, and host cells comprising the polynucleotides; and methods of using the variants.Type: ApplicationFiled: October 17, 2012Publication date: August 7, 2014Applicant: Novozymes A/SInventors: Carsten Andersen, Signe Larsen, Esben Peter Friis, Pernille Ollendorff Micheelsen, Anders Viksoe Nielsen, Randall Deinhammer, Xinyu Shen
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Publication number: 20140220642Abstract: The present invention provides isolated polypeptides having xylanase activity and polynucleotides encoding the polypeptides. The invention also provides nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides.Type: ApplicationFiled: October 26, 2012Publication date: August 7, 2014Applicant: Novozymes Inc.Inventors: Lan Tang, Ye Liu, Yu Zhang, Junxin Duan
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Publication number: 20140223608Abstract: The present invention provides isolated polypeptides having cellulolytic enhancing activity and polynucleotides encoding the polypeptides. The invention also provides nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides.Type: ApplicationFiled: September 28, 2012Publication date: August 7, 2014Applicant: Novozymes, Inc.Inventors: Yu Zhang, Junxin Duan, Lan Tang
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Publication number: 20140223602Abstract: The invention provides polypeptides having a lignocellulolytic activity, e.g., a glycosyl hydrolase, a cellulase, an endoglucanase, a cellobiohydrolase, a beta-glucosidase, a xylanase, a mannanse, a xylosidase (e.g., a ?-xylosidase), an arabinofuranosidase, and/or a glucose oxidase activity, polynucleotides encoding these polypeptides, and methods of making and using these polynucleotides and polypeptides. In one aspect, the invention provides polypeptides that can enzymatically process (hydrolyze) sugarcane bagasse, i.e., for sugarcane bagasse degradation, or for biomass processing, and polynucleotides encoding these enzymes, and making and using these polynucleotides and polypeptides. In one embodiment, the invention provides thermostable and thermotolerant forms of polypeptides of the invention.Type: ApplicationFiled: September 27, 2013Publication date: August 7, 2014Applicant: BP Corporation North America LLCInventors: Cathy Chang, Justin T. Stege, Nahla M. Aboushadi, Gordana Djordjevic, Ellen G. Burke, Peter Luginbuhl, Mark Dycaico, Toby H. Richardson, John Poland, Hefner Ying, Stacy M. Miles
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Patent number: 8795993Abstract: A process is described for producing fermentable sugars derivable from biomass that contains polysaccharide, such as cellulose, made increasingly accessible as a substrate for enzymatic degradation or other methods of depolymerization. These fermentable sugars are subsequently able to be fermented to produce various target chemicals, such as alcohols, aldehydes, ketones or acids.Type: GrantFiled: February 3, 2010Date of Patent: August 5, 2014Assignee: Hercules IncorporatedInventors: Herbert T. Conner, Patrick J. Cowan, John C. Gast
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Publication number: 20140212927Abstract: Enzyme compositions including at least a) a cocktail of cellulases from Trichoderma reesei, and b) an enzymatic cocktail from Pycnoporus cinnabarinus including a cellobiose dehydrogenase (CDH) or c) a recombinant cellobiose dehydrogenase (CDH) from Pycnoporus cinnabarinus, preferably also including a Family 61 glycoside hydrolase. Also, methods for degrading a lignocellulosic biomass, for producing a fermentation product, for producing gluconic acid, xylonic acid and/or xylobionic acid, for enhancing the production of gluconic acid, xylonic acid and/or xylobionic acid or for increasing the yield of sugars from a lignocellulosic biomass.Type: ApplicationFiled: July 4, 2012Publication date: July 31, 2014Applicants: INSTITUT NATIONAL DE LA RECHERCHE AGRONOMIQUE, ARGO INDUSTRIES RECHERCHES ET DEVELOPPEMENTS, INSTITUT FRANCAIS DU PETROLE ENERGIE NOUVELLEInventors: Jean-Claude Sigoillot, Jean-Guy Berrin, Mathieu Bey
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Publication number: 20140212930Abstract: A method of diversification of human milk oligosaccharides (HMOs) or precursors thereof, compounds obtainable by the method, and uses and compositions involving such compounds. The method comprises a) providing at least one compound or a mixture of the compounds selected from the group consisting of: optionally sialylated and/or fucosylated lactose derivatives of general formula 2 and salts thereof; b) adding at least one enzyme comprising a transglycosidase activity to the at least one compound or a mixture of compounds provided according to step a); and c) incubating the mixture obtained according to step b).Type: ApplicationFiled: May 14, 2012Publication date: July 31, 2014Applicant: GLYCOM A/SInventors: Gyula Dekany, Elise Champion, Andreas Schroven, Markus Hederos
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Publication number: 20140206045Abstract: The present invention relates to an isolated polynucleotide comprising an open reading frame encoding a polypeptide having alpha-amylase activity, the polypeptide selected from the group consisting of: a) a polypeptide comprising an amino acid sequence which has at least 70% identity with amino acids 22 to 450 of SEQ ID NO: 4; b) a polypeptide comprising an amino acid sequence which has at least 70% identity with the polypeptide encoded by the amylase encoding part of the polynucleotide inserted into a plasmid present in the E. coli host deposited under the Budapest Treaty with DSMZ under accession number DSM 15334; c) a polypeptide encoded by a polynucleotide comprising a nucleotide sequence which has at least 70% identity with the sequence shown from position 68 to 1417 in SEQ ID NO: 3; and d) a fragment of (a), (b) or (c) that has alpha-amylase activity.Type: ApplicationFiled: March 31, 2014Publication date: July 24, 2014Applicant: Novozymes A/SInventors: Lan Tang, Wenping Wu, Junxin Duan, Pia Francke Johannesen
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Publication number: 20140206046Abstract: Described herein are improved methods of degrading or converting cellulosic material into fermentable sugars using dithionite. Also described are improved methods of fermentation in the presence of dithionite.Type: ApplicationFiled: September 7, 2012Publication date: July 24, 2014Inventors: Hui Xu, Ye Chen, Xin Li, Mark Stevens, Zhengfang Kang
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Publication number: 20140206044Abstract: Biomass feedstocks (e.g., plant biomass, animal biomass, and municipal waste biomass) are processed to produce useful products, such as fuels. For example, systems are described that can convert feedstock materials to a sugar solution, which can then be fermented to produce ethanol. Biomass feedstock is saccharified in a vessel by operation of a jet mixer, the vessel also containing a liquid medium and a saccharifying agent.Type: ApplicationFiled: March 25, 2014Publication date: July 24, 2014Applicant: Xyleco, Inc.Inventors: Marshall Medoff, Thomas Craig Masterman
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Publication number: 20140206043Abstract: Biomass feedstocks (e.g., plant biomass, animal biomass, and municipal waste biomass) are processed to produce useful products, such as fuels. For example, systems are described that can convert feedstock materials to a sugar solution, which can then be fermented to produce ethanol. Biomass feedstock is dispersed in a liquid medium and then saccharified.Type: ApplicationFiled: March 25, 2014Publication date: July 24, 2014Applicant: Xyleco, Inc.Inventors: Marshall Medoff, Thomas Craig Masterman
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Patent number: 8785159Abstract: Nucleic acids encoding secretion signals, expression vectors containing the nucleic acids, and host cells containing the expression vectors are disclosed. Also disclosed are polypeptides that contain the secretion signals and methods of producing polypeptides, including methods of directing the extracellular secretion of the polypeptides. Exemplary embodiments include cellulase proteins fused to secretion signals, methods to produce and isolate these polypeptides, and methods to degrade lignocellulosic biomass.Type: GrantFiled: November 19, 2010Date of Patent: July 22, 2014Assignee: Alliance for Sustainable Energy, LLCInventors: Jeffrey G. Linger, Aldis Darzins
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Patent number: 8785158Abstract: The invention relates to a method for dissolving cellulose e.g. for the production of regenerated cellulose products such as films, fibers, particles and the like. In said method, the cellulose material is dissolved using an enzymatic treatment, followed by a base treatment. The invention is also directed to a method for producing cellulose particles wherein cellulose dissolved as described above is sprayed or mixed into a regenerating solution for precipitating cellulose particles. Moreover, the invention relates to the use of said cellulose particles produced with this method as a filler and/or coating pigment of paper and board. The invention is also directed to methods for producing and coating paper and board.Type: GrantFiled: June 27, 2006Date of Patent: July 22, 2014Assignee: Sappi Netherlands Services B.V.Inventors: Kimmo Koivunen, Petri Silenius, Janne Laine, Tapani Vuorinen
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Publication number: 20140201871Abstract: The present invention relates to isolated polypeptides having beta-glucosidase activity, beta-xylosidase activity, or beta-glucosidase and beta-xylosidase activity and isolated polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides.Type: ApplicationFiled: March 25, 2014Publication date: July 17, 2014Applicant: Novozymes Inc.Inventor: Marc Dominique Morant
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Patent number: 8778640Abstract: The present invention relates to isolated polypeptides having cellobiohydrolase activity and polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides.Type: GrantFiled: February 12, 2013Date of Patent: July 15, 2014Assignee: Novozymes Inc.Inventor: Nikolaj Spodsberg
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Patent number: 8778639Abstract: The present invention relates to isolated polypeptides having endoglucanase activity and polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides.Type: GrantFiled: February 12, 2013Date of Patent: July 15, 2014Assignee: Novozymes Inc.Inventor: Nikolaj Spodsberg
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Patent number: 8778641Abstract: The present invention relates to isolated polypeptides having cellobiohydrolase activity and polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides.Type: GrantFiled: February 12, 2013Date of Patent: July 15, 2014Assignee: Novozymes Inc.Inventor: Nikolaj Spodsberg
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Patent number: 8771994Abstract: The present invention relates to isolated polypeptides having cellobiohydrolase activity and polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides.Type: GrantFiled: February 12, 2013Date of Patent: July 8, 2014Assignee: Novozymes Inc.Inventor: Nikolaj Spodsberg
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Patent number: 8771993Abstract: The present invention relates to isolated polypeptides having endoglucanase activity and polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides.Type: GrantFiled: February 12, 2013Date of Patent: July 8, 2014Assignees: Novozymes A/S, Novozymes Inc.Inventor: Nikolaj Spodsberg
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Publication number: 20140186897Abstract: To provide a transformant of a yeast of the genus Schizosaccharomyces which can produce a modified-type ?-glucosidase with improved glucosidase activity, a method for producing such a transformant, a method for producing the modified-type ?-glucosidase, and a cellulose degradation method using the modified-type ?-glucosidase.Type: ApplicationFiled: September 19, 2013Publication date: July 3, 2014Applicant: ASAHI GLASS COMPANY, LIMITEDInventors: Katsunori OKADA, Hideki Tohda
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Publication number: 20140187825Abstract: Economic conversion of lignocellulose requires both the maximization of conversion of available carbohydrates, as well as minimization of process capital cost. Process intensification minimizes capital cost while preserving conversion yield by combining into a single step those unit operations that are conducted at similar conditions. Flowsheet variations are proposed that minimize process capital while maintaining overall conversion yield.Type: ApplicationFiled: December 20, 2013Publication date: July 3, 2014Inventors: Joseph B. Binder, Jacob Borden, Michael Chappell, Parag Shah
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Publication number: 20140186898Abstract: The invention provides processes for producing fermentable sugars from whole biomass that includes cellulose, hemicellulose, lignin, and non-lignocellulosic sugars. Some variations fractionate the whole feedstock in the presence of sulfur dioxide, a solvent for lignin, and water, to produce a liquor containing hemicellulose, cellulose-rich solids, lignin, and the non-lignocellulosic sugars. After removing the cellulose-rich solids from the liquor, the hemicellulose is hydrolyzed to hemicellulosic monomers; the cellulose-rich solids are hydrolyzed to glucose; and the hemicellulosic monomers, the glucose, and the non-lignocellulosic sugars are all recovered (separately or in combination) as fermentable sugars. The whole biomass feedstock may be selected from sugarcane, energy cane, corn, wheat, rice, sugar beets, energy beets, etc. Typical non-lignocellulosic sugars are sucrose or starch, which may be converted to monomer sugars during initial fractionation.Type: ApplicationFiled: December 30, 2013Publication date: July 3, 2014Applicant: API Intellectual Property Holdings, LLCInventors: Theodora RETSINA, Vesa PYLKKANEN, Ryan O'CONNOR
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Publication number: 20140186899Abstract: The present invention provides a process for fractionating lignocellulosic biomass, comprising: contacting biomass with SO2, water, and optionally a first solvent, to produce intermediate solids; then contacting the intermediate solids with SO2, water, and a second solvent, to produce cellulose-rich solids and a liquid phase comprising hemicelluloses and lignin. The first concentration of SO2 may be lower or higher than the second concentration of SO2. It is desirable to vary the SO2 and solvent concentrations in different stages to optimize the removal of hemicellulose versus lignin. The resulting cellulose-rich material can contain very low hemicellulose, very low lignin, or both low hemicellulose and low lignin. High-purity cellulose is useful both for producing glucose as well as for cellulose products or derivatives. The hemicelluloses may be hydrolyzed to produce monomeric sugars, and the lignin may be recovered as a co-product.Type: ApplicationFiled: December 30, 2013Publication date: July 3, 2014Applicant: API Intellectual Property Holdings, LLCInventors: Theodora RETSINA, Vesa PYLKKANEN
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Publication number: 20140186896Abstract: The invention provides recombinant GH61 proteins obtained from Myceliophtora thermophila, and nucleic acids that encode such proteins. The invention also provides protein fractions isolated from M. thermophila supernatant that have GH61 protein activity. These preparations can be used to increase yield of products from reactions in which a cellulose-containing substrate undergoes saccharification by one or more cellulase enzymes, such as endoglucanase, ?-glucosidase, or cellobiohydrolase. Combinations of GH61 protein and cellulases can be used to break down cellulosic biomass into fermentable sugars in the production of ethanol.Type: ApplicationFiled: August 22, 2011Publication date: July 3, 2014Applicant: Codexis, Inc.Inventors: Jie Yang, Louis Clark, Onorato Campopiano, Kripa Rao, Lorand Szabo, Janos Torok, Dipnath Baidyaroy
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Publication number: 20140178946Abstract: This disclosure provides for materials and methods for converting biomass to biofuels. The materials include a colloid mill with or without cellulase enzymes, and the methods include the use of a colloid mill and optionally cellulose enzymes to pretreat biomass for use in a biomass to biofuel production process.Type: ApplicationFiled: February 25, 2014Publication date: June 26, 2014Applicant: Edeniq, Inc.Inventors: Adriano Galvez, III, Glenn Richards
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Publication number: 20140178945Abstract: The present invention concerns a method of producing glucose from a granular starch substrate comprising, contacting a slurry comprising granular starch obtained from plant material with an alpha-amylase at a temperature below the starch gelatinization temperature of the granular starch to produce oligosaccharides and hydrolyzing the oligosaccharides to produce a mash comprising at least 20% glucose and further comprising fermenting the mash to obtain ethanol.Type: ApplicationFiled: December 17, 2013Publication date: June 26, 2014Applicant: Danisco US Inc.Inventors: Oreste J. LANTERO, Mian LI, Jayarama K. SHETTY
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Publication number: 20140178944Abstract: Provided are methods and compositions directed to increasing the rate and/or yield of sugar extraction processes using feedstocks. Also provided are methods and compositions for decreasing the yield of one or more undesirable products during pretreatment.Type: ApplicationFiled: June 28, 2013Publication date: June 26, 2014Inventors: Sarad Parekh, Carl P. Felice
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Publication number: 20140178947Abstract: Endoglucanase characterized by a decreased degree of activity inhibition by a lignin-derived aromatic compound, and prepared by substituting tryptophan at position 273 in the amino acid sequence of wild-type thermophilic bacterium-derived endoglucanase with an amino acid other than aromatic amino acids.Type: ApplicationFiled: March 4, 2014Publication date: June 26, 2014Applicants: National Institute of Advanced Industrial Science and Technology, Toray Industries, Inc.Inventors: Hiroyuki Kurihara, Takeshi Tsukada, Katsushige Yamada, Yumiko Mishima, Yuka Maeno, Kazuhiko Ishikawa
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Patent number: 8759033Abstract: The present invention relates to genetically modified actinobacteria for the production of an enzyme having chitosanase activity. The genetically modified actinobacteria have a reduced (or abolished) activity of the CsnR polypeptide. Such reduced activity can be obtained by reducing the capacity of expressing the csnR gene, its corresponding transcript or expressing a dominant-negative CsnR polypeptide. Such genetically modified actinobacteria are less dependent (and, in some embodiment, totally independent) on the presence of chitosan in the culture medium for producing an enzyme having chitosanase activity. In addition, the genetically modified bacteria produce less proteases in the culture medium and ultimately provide a chitosanase end-product with higher purity.Type: GrantFiled: February 8, 2013Date of Patent: June 24, 2014Assignee: Socpra Sciences et Genie S.E.C.Inventors: Ryszard Brzezinski, Marie-Pierre Dubeau
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Patent number: 8759041Abstract: The present invention relates to isolated polypeptides having xylanase activity and polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides.Type: GrantFiled: February 12, 2013Date of Patent: June 24, 2014Assignee: Novozymes Inc.Inventor: Nikolaj Spodsberg
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Patent number: 8759040Abstract: The present invention relates to isolated polypeptides having cellobiohydrolase activity and polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides.Type: GrantFiled: February 12, 2013Date of Patent: June 24, 2014Assignee: Novozymes Inc.Inventor: Nikolaj Spodsberg
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Patent number: 8759039Abstract: The present invention relates to the isolation and to the characterization of two proteins having a novel enzymatic activity, i.e. a porphyranase activity. These proteins are useful for hydrolyzing polysaccharides containing sulfated agaro-colloids and for producing oligo-porphyrans, notably oligo-porphyrans with a defined structure and size.Type: GrantFiled: July 20, 2010Date of Patent: June 24, 2014Assignee: Centre National de la Recherche Scientifique (C.N.R.S.)Inventors: Jan-Hendrik Hehemann, Gaëlle Correc, Gurvan Michel, Tristan Barbeyron, William Helbert, Mirjam Czjzek
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Publication number: 20140170713Abstract: Aspects of the AVAP® and Green Power+® technologies may be integrated, as disclosed herein. The present invention, in some variations, couples a first step of steam or hot-water extraction of biomass, with fractionation of the resulting solids using sulfur dioxide (or other acid), an alcohol (or other solvent), and water. In other variations, a first step of fractionation with sulfur dioxide (or other acid), an alcohol (or other solvent), and water is followed by treatment with steam or hot water to reduce hemicellulose content of the final solids. Some embodiments provide cellulose materials with low lignin and low hemicellulose content. Such cellulose materials are useful for making glucose by hydrolysis, as a pulp product, or as purified cellulose for making cellulose derivatives.Type: ApplicationFiled: November 12, 2013Publication date: June 19, 2014Applicant: API Intellectual Property Holdings, LLCInventors: Theodora RETSINA, Vesa PYLKKANEN
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Patent number: 8753859Abstract: This invention relates to polypeptides, more specifically amylase polypeptides and nucleic acids encoding these, and their uses e.g. as non-maltogenic exoamylases in producing food or feed products.Type: GrantFiled: November 2, 2011Date of Patent: June 17, 2014Assignee: Dupont Nutrition Biosciences APSInventors: Karsten Matthias Kragh, Anja Hemmingsen Kellet-Smith, René Mikkelsen, Rie Mejldal, Rikke L. Bundgaard Jenner
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Patent number: 8753848Abstract: A method of producing corn starch by enzymatic process involving: soaking the corn; crushing the corn, separating and washing embryo; fine grinding; washing and drying fiber; separating and drying protein; washing, dewatering and drying the starch. An enzyme preparation is added before the step of washing, dewatering and drying the starch; the enzyme preparation is cellulose, or xylanase, or combination of the cellulose and the xylanase; and addition of the enzyme preparation is from 0.001% to 0.08% by weight of the corn. Based on the technology of traditional wet process, the method of the present invention comprises a step of adding enzyme preparation in the process of separating the corn, which improves the effect and the efficiency of mechanical separation, and further improves the purity and yield of the substance separated while also reducing the energy consumption.Type: GrantFiled: January 14, 2014Date of Patent: June 17, 2014Assignee: Baiyin Sino Biotechnology Co., Ltd.Inventors: Xinmin He, Feng Yu, Shuangjing Wang, Qiang Cui, Weiguo Sun
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Patent number: 8753860Abstract: The present invention relates to isolated polypeptides having cellobiohydrolase activity and isolated polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides.Type: GrantFiled: February 12, 2013Date of Patent: June 17, 2014Assignee: Novozymes A/SInventors: Marc D. Morant, Paul Harris
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Publication number: 20140162323Abstract: A genetically modified organism comprising: at least one nucleic acid sequence and/or at least one recombinant nucleic acid isolated from Alicyclobacillus acidocaldarius and encoding a polypeptide involved in at least partially degrading, cleaving, transporting, metabolizing, or removing polysaccharides, cellulose, lignocellulose, hemicellulose, lignin, starch, sugars, sugar oligomers, carbohydrates, complex carbohydrates, chitin, heteroxylans, glycosides, xylan-, glucan-, galactan-, or mannan-decorating groups; and at least one nucleic acid sequence and/or at least one recombinant nucleic acid encoding a polypeptide involved in fermenting sugar molecules to a product. Additionally, enzymatic and/or proteinaceous extracts may be isolated from one or more genetically modified organisms. The extracts are utilized to convert biomass into a product.Type: ApplicationFiled: February 20, 2014Publication date: June 12, 2014Inventors: David N. Thompson, William A. Apel, Vicki S. Thompson, Thomas E. Ward
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Publication number: 20140158642Abstract: A method of producing a filter aid includes: (A) obtaining a pretreated biomass by pulverization treatment and/or thermochemical treatment of a cellulose-containing biomass; (B) treating the pretreated biomass obtained in (A) with cellulase to obtain a cellulase-treated product; and (C) obtaining a solid content of the cellulase-treated product of (B).Type: ApplicationFiled: July 27, 2012Publication date: June 12, 2014Applicant: TORAY INDUSTRIES, INC.Inventors: Atsushi Minamino, Hiroyuki Kurihara, Katsushige Yamada, Junpei Kishimoto