Carrier Is Synthetic Resin Patents (Class 436/531)
  • Patent number: 7238536
    Abstract: A membrane comprising two or more macromolecules that interact via multiple reversible physical interaction points (MRIPM). The membrane further comprises a chemical species A, which interacts selectively or nonselectively with the MRIPM; and a chemical species B, which interacts selectively with the MRIPM whereby the passage of species A across the MRIPM is moderated by species B.
    Type: Grant
    Filed: March 22, 2005
    Date of Patent: July 3, 2007
    Assignee: Florida State University Research Foundation, Inc.
    Inventor: Joseph B. Schlenoff
  • Patent number: 7232661
    Abstract: This invention is about a method for the prenatal diagnosis of preterm delivery, fetal infection, and fetal damage, and diagnostic reagent system and diagnostic kit for the diagnosis. The method, diagnostic reagent system, and kit are based on the finding that the level of MMP-8 in the amniotic fluid is significantly higher when the pregnant woman is at risk for preterm delivery, intrauterine infection, and fetal damage. The diagnostic reagent system and kit can be applied to patients with or without clinical signs of preterm labor or premature rupture of fetal membranes. With its superiority in sensitivity and specificity as well as its less invasiveness compared to the conventional method of measuring fetal blood cytokine levels, this diagnostic reagent system and kit is very useful in the prenatal diagnosis of preterm delivery, fetal infection, and fetal damage.
    Type: Grant
    Filed: August 1, 2001
    Date of Patent: June 19, 2007
    Assignee: Seoul National University Industry Foundation
    Inventor: Bo Hyun Yoon
  • Patent number: 7229840
    Abstract: The object with the funtionalized hydrogel surface for detection of analyte molecules is obtainable by a process including (1) providing a hydrogel layer on a base surface; (2) bonding organic molecules to the hydrogel layer, each including a chain of atoms with a chain length of at least 8 atoms and a terminal group, which is an amine group, a carboxylic acid group, or a derivative thereof, and (3) reacting the terminal group with a receptor molecule that contains at least three groups, each of which is an amine group, a carboxylic acid group, a sulfonic acid group, a phosphoric acid group, a phosphonic acid group, or a derivative thereof. The receptor molecule can be a protein and the hydrogel can include a water-swellable organic polymer. A method of detecting analyte molecules, for example by surface resonance spectroscopy, is also part of the invention.
    Type: Grant
    Filed: November 2, 2000
    Date of Patent: June 12, 2007
    Inventor: Erik Wischerhoff
  • Patent number: 7229835
    Abstract: A compound linked to a solid support (R) through a divalent linker moiety (X) and which is represented by the following formula: is disclosed. In particular, the 1-hydroxybenzotriazole-6-carboxylic acid is directly linked to the support under mild conditions (i.e., in aqueous or organic solvents at neutral pH and at room temperature). The polymer bound 1-hydroxybenzotriazole-6-carboxylic acid can be used for the derivatization of amines as well as for single step amino group modification of proteins, peptides, and amines via acylation or sulfonylation reactions. A flow through device and method for the single step amino group modifications of proteins, peptides, and amines is disclosed. Also disclosed is a flow through device for the detection of amines in a sample. Additionally, a device and method for the detection of amines in a sample using 1-hydroxybenzotriazole-6-carboxylic acid is disclosed. In a preferred embodiment, the device is used to detect the presence of amines in a spoiled meat product.
    Type: Grant
    Filed: October 25, 2001
    Date of Patent: June 12, 2007
    Assignee: The University of Maryland, Baltimore County
    Inventor: Aristotle G. Kalivretenos
  • Patent number: 7229771
    Abstract: The use of polyalkylene oxide modified reagents is disclosed in a method for the detection of an analyte or in suitable reagent kits for such methods.
    Type: Grant
    Filed: November 2, 1998
    Date of Patent: June 12, 2007
    Assignee: Roche Diagnostics GmbH
    Inventors: Hans Hornauer, Peter Sluka, Johann Karl, Helmut Lenz, Wolfgang Mutter
  • Patent number: 7226753
    Abstract: The present invention relates to displacement assay type bioassay materials useful for the detection of toxic substances and, more particularly, to packaging materials for food and other products, along with methods for their manufacture and use. The invention provides a unique composite material capable of detecting and identifying multiple biological materials within a single package. The biological material identification system is designed for incorporation into existing types of flexible packaging material such as polyvinylchloride or polyolefin films, and its introduction into the existing packaging infrastructure will require little or no change to present systems or procedures.
    Type: Grant
    Filed: January 28, 2004
    Date of Patent: June 5, 2007
    Assignee: Toxin Alert, Inc.
    Inventor: William T. Bodenhamer
  • Patent number: 7223534
    Abstract: A biosensor includes a substrate with a layer of receptive material disposed thereon. The receptive material is specific for an analyte of interest. A pattern of active and deactivated areas of the receptive material are defined in the receptive material layer by a masking process.
    Type: Grant
    Filed: May 3, 2002
    Date of Patent: May 29, 2007
    Assignee: Kimberly-Clark Worldwide, Inc.
    Inventors: Rosann Kaylor, David Cohen, Curtis Sayre
  • Patent number: 7217575
    Abstract: A covalent conjugate of a 4?-hydroxyazobenzene-2-carboxylic acid derivative (HABA) and an avidin-type molecule, of the formula: wherein A is (CH2)n or —CH?CH—, wherein n is an integer from 0–10; B is (CH2)n wherein n is an integer from 2 to 10; m is zero or 1; and Av is the residue of an avidin-type molecule selected from the group comprising native egg-white avidin, recombinant avidin, deglycosylated avidins, bacterial streptavidin, recombinant streptavidin, truncated streptavidin and other derivatives of said avidin-type molecules. These HABAylated avidins are red colored in the quinone configuration and can be used in many applications in the avidin-biotin technology.
    Type: Grant
    Filed: July 23, 2003
    Date of Patent: May 15, 2007
    Assignee: Yeda Research and Development Co. Ltd.
    Inventors: Meir Wilchek, Edward A Bayer, Heike Hofstetter, Margherita Morpurgo
  • Patent number: 7214546
    Abstract: Chemiluminescent substrate delivery systems comprising a conjugate of a dendrimer and at least one chemiluminescent substrate are provided. The substrate delivery systems can also include a chemiluminescence enhancer. The dendrimer/chemiluminescent substrate conjugates can be used in kits including an enzyme capable of activating the chemiluminescent substrate to produce a peroxygenated intermediate that decomposes to produce light. The dendrimer/chemiluminescent substrate conjugates can be used in assays to detect the presence of an analyte (e.g., an enzyme, an antibody, an antigen or a nucleic acid) in a sample.
    Type: Grant
    Filed: January 8, 2002
    Date of Patent: May 8, 2007
    Assignee: Applera Corporation
    Inventor: Alison L. Sparks
  • Patent number: 7211445
    Abstract: A method is provided for covalently linking carbohydrates, proteins, nucleic acids, and other biomolecules under neutral conditions, using a Diels-Alder cycloaddition reaction. In an example, activated carbon-carbon double bonds were attached to free amino sites of a carrier protein, and a conjugated diene was attached to a carbohydrate hapten. Spontaneous coupling of the carbohydrate and the protein components under very mild conditions provided glycoconjugates containing up to 37 carbohydrate hapten units per carrier protein molecule. The method is also applicable to the immobilization of biomolecules on gel or solid supports. The conjugated products are useful as immunogens and as analytical and diagnostic reagents.
    Type: Grant
    Filed: October 22, 2003
    Date of Patent: May 1, 2007
    Assignee: The United States of America as Represented by the Secretary of the Department of Health and Human Services
    Inventor: Vince Pozsgay
  • Patent number: 7208286
    Abstract: Monolithic bioelectronic devices for the detection of ammonia includes a microorganism that metabolizes ammonia and which harbors a lux gene fused with a heterologous promoter gene stably incorporated into the chromosome of the microorganism and an Optical Application Specific Integrated Circuit (OASIC). The microorganism is generally a bacterium.
    Type: Grant
    Filed: June 14, 2005
    Date of Patent: April 24, 2007
    Assignee: UT-Battelle LLC
    Inventors: Michael L. Simpson, Michael J. Paulus, Gary S. Sayler, Bruce M. Applegate, Steven A. Ripp
  • Patent number: 7208299
    Abstract: The present invention is directed to a composition and method for regulating the adhesion of cells and biomolecules to hydrophobic surfaces and hydrophobic coated surfaces. The composition is a biomolecule conjugated end-group activated polymer (EGAP). The biomolecule conjugated EGAP can be put to numerous uses including cell adhesion, cell growth, cell sorting, and other biological assays.
    Type: Grant
    Filed: December 30, 2003
    Date of Patent: April 24, 2007
    Assignee: Universtiy of Utah Research Foundation
    Inventors: Karin D. Caldwell, Patrick A. Tresco, Jennifer Neff
  • Patent number: 7205162
    Abstract: Artificial antibodies or antibody mimics are described. They consist of polymers that carry specific binding sites mimicking the properties of antibodies. There is also described a method for producing artificial antibodies, in which polymerisable monomers carrying functional groups and crosslinking monomers are polymerised in the presence of a print molecule and subsequently the print molecule is removed leaving specific binding sites complementary to the print molecules. There are also described methods for determination and isolation of organic molecules using the artificial antibodies as well as therapeutic and diagnostic methods using these antibodies.
    Type: Grant
    Filed: May 6, 1999
    Date of Patent: April 17, 2007
    Inventors: Klaus Mosbach, Georg Vlatakis, Lars I. Andersson, Ralf Müller
  • Patent number: 7205159
    Abstract: A method and apparatus for use in a flow through assay process is disclosed. The method is characterised by a “pre-incubation step” in which the sample which is to be analysed, (typically for the presence of a particular protein), and a detection analyte (typically an antibody bound to colloidal gold or a fluorescent tag) which is known to bind to the particular protein may bind together for a desired period of time. This pre incubation step occurs before the mixture of sample and detection analyte come into contact with a capture analyte bound to a membrane. The provision of the pre-incubation step has the effect of both improving the sensitivity of the assay and reducing the volume of sample required for an assay. An apparatus for carrying out the method is disclosed defining a pre-incubation chamber for receiving the sample and detection analyte having a base defined by a membrane and a second membrane to which a capture analyte is bound.
    Type: Grant
    Filed: August 20, 2002
    Date of Patent: April 17, 2007
    Assignee: Proteome Systems Intellectual Property Pty Ltd.
    Inventors: Robert Alan Cole, Andrew John Sloane, William Samuel Hunter
  • Patent number: 7205161
    Abstract: Sensors for determining the presence and concentration of biomolecules in a biological sample are provided in the form of polymer brushes, which comprise a substrate having a surface modified with a hydrophobic polymer segment, attached to which is a water-dispersible or water-soluble polymer segment having functional groups that bind probes. The method of synthesis of such sensors preferably includes use of controlled free radical polymerization techniques, which allows for controlled architecture polymers to modify the surface of the substrate, and the use of monomers possessing functional groups which do not require activation prior to probe attachment. In this manner functional groups in the polymer chain are removed from the surface, which allows for solution chemistry to be more realistically reproduced with the benefits of a solid bound probe.
    Type: Grant
    Filed: January 10, 2002
    Date of Patent: April 17, 2007
    Assignee: Symyx Technologies, Inc.
    Inventors: Gerrit Klaerner, Didier Benoit, Dominique Charmot, Srinivas Nomula, Marcelo Eduardo Piotti, Laura T. Mazzola
  • Patent number: 7195925
    Abstract: The invention relates to a stimulus-responding polymer obtained by polymerizing at least a monomer represented by a general formula (1) and a monomer represented by a general formula (2), and a separation method or concentration method of microorganisms, a purification method, detection method or concentration method of nucleic acids, a separating agent, a separation method of biomaterials and a conversion method of materials, which use this polymer, (in the formula, R11 represents hydrogen atom or methyl group, and R12 represents single bond or a straight or branched alkylene group having from 1 to 5 carbon atoms) (in the formula, R13 represents hydrogen atom or methyl group, and R14 represents hydrogen atom, a straight, branched or cyclic alkyl group, alkoxyl group or alkylamino group having from 1 to 10 carbon atoms, an aryl group or a heterocyclic group).
    Type: Grant
    Filed: August 20, 2001
    Date of Patent: March 27, 2007
    Assignees: National Institute of Advanced Industrial Science and Technology, Chisso Corporation
    Inventors: Noriyuki Ohnishi, Hirotaka Furukawa, Kazunori Kataoka, Katsuhiko Ueno
  • Patent number: 7195882
    Abstract: The present invention relates to monoclonal antibodies that specifically bind buprenorphine and/or at least one metabolic product thereof. The present invention further relates to buprenorphine metabolite conjugates for the production of monoclonal antibodies that specifically bind buprenorphine and/or at least one metabolic product thereof and hybridoma cells that produce the monoclonal antibodies. The invention also relates to immunoassay methods for determining buprenorphine and/or one or more buprenorphine metabolites in a sample using the novel antibodies and conjugates of the present invention.
    Type: Grant
    Filed: June 3, 2003
    Date of Patent: March 27, 2007
    Assignee: Roche Diagnostics Operations, Inc.
    Inventors: Richard T. Root, Mitali Ghoshal
  • Patent number: 7192786
    Abstract: This invention relates to a biospecific assay method, in which microparticles coated with the bioaffinity reactant A binding the analyte to be assayed; the sample to be analyzed, and the labelled bioaffinity reactant B are mixed. After the binding reaction the signal strength from the labelled bioaffinity reactant B bound to the microparticles is quantitated for the determination of the concentration of the analyte in the sample. According to the invention, such an amount of sample and microparticles is used in the assay that after binding of the analyte of the sample to the said amount of microparticles, each individual microparticle will emit such a signal strength as to allow the measurement of the analyte concentration over the whole range of typical analyte concentrations, and the signal strength from each microparticle is measured separately.
    Type: Grant
    Filed: June 7, 1995
    Date of Patent: March 20, 2007
    Assignee: Wallac Oy
    Inventors: Timo Nils-Erik Lövgren, Antti Juhana Iitiä, Kim Sverker Immanuel Pettersson
  • Patent number: 7186567
    Abstract: A method of sensing an environmental agent, comprising obtaining a sample from the environment and transferring the sample into the working fluid for dispensation to a detection module. The sample and working fluid mixture is filtered through a porous polymer Bragg grating. By comparing the refractive index of the grating with the mixture to the refractive index of a grating without the sample, a difference in the refractive index aids in the identification of a hazardous agent in the environment. The sensor also acts as a chemical filter by trapping specific target agents by a highly specific reaction with a conjugate molecule. Recirculation of the working fluid throughout the system provides a sensor that is “always on.
    Type: Grant
    Filed: November 4, 2005
    Date of Patent: March 6, 2007
    Assignee: Science Applications International Corporation
    Inventors: Richard L. Sutherland, Donna M. Brandelik, Christina K. Shepherd
  • Patent number: 7172905
    Abstract: A substrate comprises a surface, and a plurality of moieties, on at least a portion of the surface. The moieties are moieties of formula: Surf-L—Q—T, where —T comprises a reactant ligand, and Surf- designates where the moiety attaches to the surface. The substrate can be made into a protein chip by the reaction of a reactant ligand and a fusion polypeptide, where the fusion polypeptide includes a capture polypeptide moiety which corresponds to the reactant ligand.
    Type: Grant
    Filed: August 7, 2001
    Date of Patent: February 6, 2007
    Assignee: The University of Chicago
    Inventors: Milan Mrksich, Christian Hodneland
  • Patent number: 7153681
    Abstract: A reagent useful in immunoassays, comprising a direct particulate level co-sensitised with a specific binding agent having specificity for an analyte or analyte analogue and with a non-specific protein which can participate in a control reaction with another specific binding agent which does not bind to the first specific binding agent nor participate in the formation of a complex by means of which detection of the analyte or analyte analogue is accomplished. Preferably the first specific binding agent is an antibody raised in a first species and the non-specific protein is an immunoglobulin from another species. Optionally, the reagent additionally comprises a second population of the direct particulate label sensitised solely with the non-specific protein.
    Type: Grant
    Filed: April 25, 2000
    Date of Patent: December 26, 2006
    Assignee: Inverness Medical Switzerland GmbH
    Inventors: Yvonne E. Penfold, David A. Percival
  • Patent number: 7144743
    Abstract: For certain mixed mode resins having anionic character, a ligand is joined to a solid support via a linkage that includes a mercapto-, ether- or amino-containing moiety. A suitable ligand comprises an aromatic group, a heteroaromatic group, or a heterocyclic group, optionally fused, that is sulfate-, sulfonate-, phosphonate- or phosphate-substituted and that is linked to such a moiety. These resins possess an anionic character under conditions prescribed for their use. Separation of a biological substance, such as a peptide or protein, can be accomplished with a resin of this type via a change in the pH of eluants, thereby effecting adsorption and desorption.
    Type: Grant
    Filed: September 12, 2003
    Date of Patent: December 5, 2006
    Assignee: Pall Corporation
    Inventors: Egisto Boschetti, Pierre Girot
  • Patent number: 7144742
    Abstract: This invention provides solid phase specific binding lateral flow assay methods, devices and kits for quantitating high and low molecular weight analytes. The methods and devices of the invention employ labelled reagents which are either analyte analogs or complementary specific binding pair members for the analyte and a novel arrangement of capture zones comprising immobilized specific binding substances for either the analyte or the labelled reagent to effect bound from unbound labelled reagent as a function of analyte concentration. The capture zones are disposed on a non-bibulous matrix defining a flow path from a sample receiving zone to the capture zone. The devices of this invention also include multilane flow paths and multiple capture zones to quantitate analyte.
    Type: Grant
    Filed: December 17, 2004
    Date of Patent: December 5, 2006
    Assignee: Quidel Corporation
    Inventors: Hans Boehringer, Gerald Rowley, Allan D. Pronovost
  • Patent number: 7141437
    Abstract: Solid-state colorimetric biosensors having sensory groups and interdendritic cross-linking segments of alternating conjugated double and triple bonds are prepared by intermolecular polymerization of diacetylene-functionalized dendritic polymer precursors. The polymerization process may be used to form solid films that are capable of indicating the presence of an analyte by a detectable change in color. The disclosed solid-state colorimetric biosensors may exhibit excellent stability at elevated temperatures and in the presence of organic solvents, and due to the dendritic architecture and high density of sensing functionality achieve high sensitivity to analytes.
    Type: Grant
    Filed: November 13, 2003
    Date of Patent: November 28, 2006
    Assignee: Michigan Molecular Institute
    Inventors: Petar R. Dvornic, Steven N. Kaganove, Abhijit Sarkar
  • Patent number: 7135343
    Abstract: The present invention relates to bio-molecule resistant surfaces for use in assays, particularly in assay devices such as arrays and microfluidic devices. The biomolecule resistant surface of the present invention are prepared by coating a substrate with hydrophilic terminated alkoxysilanes having formula (A): wherein R is an alkyl group of a size that allows for sufficient hydrolysis and n is 1, 2 or 3; R1 is a hydrophilic moiety; LC is a C1 to a C10 linker chain consisting of a group selected from alkyl, aryl, alkaryl and aralkyl and m is 1, 2, or 3; R2 is a C1 to a C7 alkyl group and x is 0, 1, or 2; and m+n+x is equal to 4.
    Type: Grant
    Filed: June 17, 2002
    Date of Patent: November 14, 2006
    Assignee: Agilent Technologies, Inc.
    Inventors: Dan-Hui Yang, Namyong Kim, Janelle Gunther
  • Patent number: 7132295
    Abstract: This application relates to isotopically labeled affinity markers of the formula (II) for mass spectrometric analysis of proteins. In formula (II), the groups A, PRG, S, Z, L', Z', R, R', k, l, m, and n are as defined in the claims. The application also provides a process for preparing these materials, a method for analyzing proteins using such materials, and a kit containing one or more of these materials.
    Type: Grant
    Filed: October 30, 2002
    Date of Patent: November 7, 2006
    Assignee: Bayer Aktiengesellschaft
    Inventors: Hans-Georg Lerchen, Hans-Ulrich Siegmund, Dorian Immler, Andreas Schumacher, Daniel Auriel
  • Patent number: 7122323
    Abstract: An apparatus and method for synthesizing a combinatorial library comprising a plurality of chemical compounds such that the chemical composition of each compound is easily tracked. The library compounds are synthesized on solid-phase supports, which are spatially arranged in frames during synthesis according to a predetermined protocol, such that each solid-phase support passes through a series of unique spatial 2D or 3D addresses by which the chemical composition of each compound may be determined at any point during synthesis. Solid-phase supports include hollow tubular-shaped lanterns and gears.
    Type: Grant
    Filed: December 11, 2002
    Date of Patent: October 17, 2006
    Assignee: Aventis Pharmaceuticals Inc.
    Inventors: Marcel Patek, Safar Pavel, Martin Smrcina, Eric Wegrzyniak, Peter Strop, Gary A Flynn, Stephen A Baum
  • Patent number: 7122356
    Abstract: A method for making a medical device having at least one biomolecule immobilized on a substrate surface is provided. One method of the present invention includes immobilizing a biomolecule comprising an unsubstituted amide moiety on a biomaterial surface. Another method of the present invention includes immobilizing a biomolecule on a biomaterial surface comprising an unsubstituted amide moiety. Still another method of the present invention may be employed to crosslink biomolecules comprising unsubstituted amide moieties immobilized on medical device surfaces. Additionally, one method of the present invention may be employed to crosslink biomolecules comprising unsubstituted amide moieties in solution, thereby forming a crosslinked biomaterial or a crosslinked medical device coating.
    Type: Grant
    Filed: July 15, 2003
    Date of Patent: October 17, 2006
    Assignee: Medtronic, Inc.
    Inventors: James R. Keogh, Paul V. Trescony
  • Patent number: 7122381
    Abstract: A selective adsorption material made by the process comprising: a) non-cavalently or reversibly-covalently binding a print molecule to at least two styrene, acrylate or silica monomers, each of which monomers brinds to said print molecule by means of a different functional group; b) immobilizing said bound monomers by being polymerized to each other in the presence of an effective amount of cross-linker; and c) removing said print molecule from said polymer by extraction to leave a cavity in said polymer which is stereo-tailored to a biological molecule of interest; and wherein said print molecule is the biological molecule of interest.
    Type: Grant
    Filed: September 4, 1992
    Date of Patent: October 17, 2006
    Inventors: Magnus Glad, Maria Kempe, Klaus Mosbach
  • Patent number: 7112453
    Abstract: This invention provides methods of retentate chromatography for resolving analytes in a sample. The methods involve adsorbing the analytes to a substrate under a plurality of different selectivity conditions, and detecting the analytes retained on the substrate by desorption spectrometry. The methods are useful in biology and medicine, including clinical diagnostics and drug discovery.
    Type: Grant
    Filed: August 5, 2002
    Date of Patent: September 26, 2006
    Assignee: Ciphergen Biosystems, Inc.
    Inventors: T. William Hutchens, Tai-Tung Yip
  • Patent number: 7109024
    Abstract: This invention features a biomolecule-bound substrate that includes a support made of an organic polymer and having an unmodified surface; and a plurality of unmodified biomolecules immobilized on the unmodified surface. The organic polymer is acrylic resin, polypropylene, polystyrene, polyethylene, polyvinyl chloride, polysulfone, polycarbonate, cellulose acetate, rubber, latex, polyethylene terephthalate, acrylonitrile butadiene styrene, acrylonitrile styrene, or a combination thereof. The substrate is formed by placing the unmodified biomolecules on the unmodified surface followed by ultraviolet irradiation.
    Type: Grant
    Filed: April 7, 2003
    Date of Patent: September 19, 2006
    Assignee: Dr. Chip Biotechnology Inc.
    Inventors: Kan-Hung Lee, Yu-Hau Shih, Chuan-Mei Tsai, Yih-Weng Wang, Hsiung Hsiao, Chi-Horng Bair, Shin-Hwan Wang
  • Patent number: 7105289
    Abstract: The present invention provides imprint bead compositions useful for capturing, isolating, detecting, analyzing and/or quantifying molecules in a sample. The imprint bead compositions comprise a matrix material having imprint cavities of a template molecule or molecules imprinted thereon wherein a substantial number of the imprint cavities are located at or near the surface of the matrix material.
    Type: Grant
    Filed: April 11, 2002
    Date of Patent: September 12, 2006
    Assignee: Aspira Biosystems, Inc.
    Inventors: Chin-Shiou Huang, Casey C. Lynch, Alexander Strikovsky
  • Patent number: 7101660
    Abstract: The invention provides a methods for fabricating colorimetric resonant reflection biosensors.
    Type: Grant
    Filed: July 15, 2002
    Date of Patent: September 5, 2006
    Assignee: SRU Biosystems, Inc.
    Inventors: Brian T. Cunningham, Jean Qiu
  • Patent number: 7090993
    Abstract: This invention relates to a method of assaying pyrrole-containing biological compounds and chemical compositions that can be used in the method. The method involves contacting a biological compound with one of: a) a bound or bindable derivatizing agent which forms a reaction product with the biological compound, followed by exposure to a detectable molecule which forms a complex with the reaction product; or b) a derivatizing agent which forms a reaction product with the biological compound, followed by exposure to a bound binding agent specific to the biological compound in the reaction product; or c) a binding agent specific to the biological compound, followed by exposure to a derivatizing agent which forms a reaction product with the biological compound, and determining the amount of bound biological compound. There is also provided a method of preparing an antigen.
    Type: Grant
    Filed: September 26, 2003
    Date of Patent: August 15, 2006
    Assignee: Rowett Research Institute
    Inventors: Jeffrey D. Brady, Simon P. Robins
  • Patent number: 7090984
    Abstract: A method for detecting a substance, comprising developing a developing liquid through a test region up to a reference region, and a detection device for use therein, wherein the reference region comprises a metal compound other than an alkali metal salt, and the developing liquid that reaches the reference region contains a label that can be accumulated in the reference region. The label is preferably a colored particle, and is preferably bound to an antibody or an antigen.
    Type: Grant
    Filed: October 25, 2001
    Date of Patent: August 15, 2006
    Assignee: Morinaga Milk Industry Co., Ltd.
    Inventors: Koichi Hashimoto, Yoshitsugu Harada, Shigetoshi Okubo
  • Patent number: 7091049
    Abstract: An enhanced diffraction based biosensor system and method are provided for detecting an analyte of interest in a test medium. The system incorporates at least one additional detection tag substance with the analyte of interest, the tag emitting a measurable parameter that is different from optical diffraction characteristics of the analyte. The biosensor may be a “fluoroptical” system wherein the detection tag is a fluorescence emitting substance, including fluorescent-labeled diffraction enhancing elements. The enhanced diffraction biosensor system may determine the presence of analytes in biological fluids both qualitatively and quantitatively.
    Type: Grant
    Filed: June 26, 2002
    Date of Patent: August 15, 2006
    Assignee: Kimberly-Clark Worldwide, Inc.
    Inventors: Rameshbabu Boga, Chibueze Obinna Chidebelu-Eze, Rosann M. Kaylor, Xuedong Song
  • Patent number: 7081365
    Abstract: The invention concerns scintillation proximity assays performed in multiwell plates where a charge coupled device is used to image the wells. Conventional phosphors emit blue light (350–450 nm) which is absorbed by yellow or brown assay components. This problem is addressed by the use of phosphors that emit radiation of longer wavelength (480–900 nm).
    Type: Grant
    Filed: December 13, 2002
    Date of Patent: July 25, 2006
    Assignee: GE Healthcare Limited
    Inventor: Robert A. Jessop
  • Patent number: 7070941
    Abstract: The invention provides methods and compositions for azide tagging of biomolecules. In one embodiment of the invention, proteins are tagged by metabolic incorporation of prenylated azido-analog substrates. Examples of such analogs are azido farnesyl diphosphate and azido farnesyl alcohol. The azido moiety in the resulting modified proteins provides an affinity tag, which can be chemoselectively captured by an azide-specific conjugation reaction, such as the Staudinger reaction, using a phosphine capture reagent. When the capture agent is biotinylated, the resulting conjugates can be detected and affinity-purified by streptavidin-linked- HRP and streptavidin-conjugated agarose beads, respectively. The invention allows detection and isolation of proteins with high yield, high specificity, and low contamination without harsh treatment of proteins.
    Type: Grant
    Filed: November 17, 2003
    Date of Patent: July 4, 2006
    Assignee: Board of Regents, The University of Texas System
    Inventors: Yingming Zhao, John R. Falck
  • Patent number: 7071006
    Abstract: Carriers hold remote-acting bodies which can be manipulated by a remote force, and also hold a micro-substance which is a target substance of an assay. The remote-acting bodies are manipulated in order to control the positions of the micro-substances, so as to execute assays for various target substances efficiently, at low cost, easily, and reliably. Various aspects of interest include the carriers which hold the micro-substances, a system suspending the carriers, an apparatus for manipulating the carriers, and a method of controlling the position of the carriers.
    Type: Grant
    Filed: December 27, 2001
    Date of Patent: July 4, 2006
    Assignee: Precision System Science Co., Ltd.
    Inventors: Hideji Tajima, Yoshiro Okami
  • Patent number: 7070987
    Abstract: Methods and compositions are provided for detecting biomolecular interactions. The use of labels is not required and the methods can be performed in a high-throughput manner. The invention also provides optical devices useful as narrow band filters.
    Type: Grant
    Filed: January 28, 2002
    Date of Patent: July 4, 2006
    Assignee: SRU Biosystems, Inc.
    Inventors: Brian T. Cunningham, Jane Pepper, Bo Lin, Peter Li, Jean Qiu, Homer Pien
  • Patent number: 7067267
    Abstract: This invention relates to a method of assaying pyrrole-containing biological compounds and chemical compositions that can be used in the method. The method involves contacting a biological compound with one of: a) a bound or bindable derivatizing agent which forms a reaction product with the biological compound, followed by exposure to a detectable molecule which forms a complex with the reaction product; or b) a derivatizing agent which forms a reaction product with the biological compound, followed by exposure to a bound binding agent specific to the biological compound in the reaction product; or c) a binding agent specific to the biological compound, followed by exposure to a derivatizing agent which forms a reaction product with the biological compound, and determining the amount of bound biological compound. There is also provided a method of preparing an antigen.
    Type: Grant
    Filed: September 26, 2003
    Date of Patent: June 27, 2006
    Assignee: Rowett Research Institute
    Inventors: Jeffrey D. Brady, Simon P. Robins
  • Patent number: 7067270
    Abstract: An object of the present invention is to provide a method for detecting a protein using a nonradioactive label which is prevented to a smaller extent than in the case of the interaction of biotin/(strepto)avidin, and which achieves higher detection sensitivity than that of the detection system using DIG. The present invention provides a method for detecting a target substance, which comprises steps of: (A) allowing a target substance to come into contact with a protein labeled with a compound having a 6-membered ring, so as to carry out a binding reaction; and (B) detecting the protein labeled with the compound having a 6-membered ring, which was bound to the target substance, by using an antibody against the above compound having a 6-membered ring.
    Type: Grant
    Filed: December 13, 2004
    Date of Patent: June 27, 2006
    Assignee: Fuji Photo Film Co., Ltd.
    Inventor: Yukio Sudo
  • Patent number: 7067324
    Abstract: The present invention provides a method for reducing undesirable light emission from a sample using at least one photon producing agent and at least one photon reducing agent (e.g. dye-based photon reducing agents). The present invention further provides a method for reducing undesirable light emission from a sample (e.g., a biochemical or cellular sample) with at least one photon producing agent and at least one collisional quencher. The present invention also provides a method for reducing undesirable light emission from a sample (e.g., a biochemical or cellular sample) with at least one photon producing agent and at least one quencher, such as an electronic quencher. The present invention also provides a system and method of screening test chemicals in fluorescent assays using photon reducing agents. The present invention also provides compositions, pharmaceutical compositions, and kits for practicing these methods.
    Type: Grant
    Filed: January 12, 2001
    Date of Patent: June 27, 2006
    Assignee: Invitrogen Corporation
    Inventors: Tom Knapp, Gregor Zlokarnik, Paul Negulescu, Roger Y. Tsien, Tim Rink
  • Patent number: 7063952
    Abstract: In a process for the quantitative optical analysis of fluorescently labelled biological cells 5, a cell layer on a transparent support at the bottom 2 of a reaction vessel 1 is in contact with a solution 3 containing the fluorescent dye 4. The sensitivity of analytical detection can be considerably improved if to the fluorescent dye 4 already present in addition a masking dye 9, which absorbs the excitation light 6 for the fluorescent dye 4 and/or its emission light 7, is added to the solution 3 and/or if a separating layer 10 permeable to the solution and absorbing and/or reflecting the excitation light 6 or the emission light 7 is applied to the cell layer at the bottom 2. This process can also be used for improving the sensitivity in the quantitative optical analysis of a luminescent biological cell layer. The separating layer 10 must in this case be composed such that it has a high power of reflection for the luminescent light 11.
    Type: Grant
    Filed: September 28, 2001
    Date of Patent: June 20, 2006
    Assignee: Bayer Healthcare AG
    Inventors: Thoams Krahn, Wolfgang Paffhausen, Andreas Schade, Martin Bechem, Delf Schmidt
  • Patent number: 7060505
    Abstract: An assay device and method are provided which allow the determination of the presence or absence of at least one analyte in a test sample, while providing specific identification of the test subject. The assay device includes a reaction medium having at least one reaction zone and at least one control zone, which is capable of providing a pattern suitable for identifying the test subject. The pattern suitable for identifying the test subject is preferably a fingerprint. In a preferred embodiment of the invention, the reaction zone and the control zone include at least one member of a ligand/receptor pair.
    Type: Grant
    Filed: March 8, 2004
    Date of Patent: June 13, 2006
    Assignee: La Mina, Inc.
    Inventor: Raouf Guirguis
  • Patent number: 7060507
    Abstract: Targeted molecular bar codes and methods for using the same are provided. The subject targeted molecular bar codes include a molecular bar code and a member of a specific binding pair, where the specific binding pair member is generally bonded to the bar code through a linking group. The subject molecular bar code may be read during translocation through a single nano-meter scale pore. The subject targeted molecular bar codes find use in a variety of different applications involving analyte detection, such as screening and diagnostic applications.
    Type: Grant
    Filed: August 13, 2002
    Date of Patent: June 13, 2006
    Assignee: The Regents of the University of California
    Inventors: Mark Akeson, David W. Deamer, Wenonah Vercoutere, Hugh E. Olsen, Rebecca Braslau, Bakthan Singaram, Derek Steiner, Frank Cappuccio
  • Patent number: 7056657
    Abstract: Separation apparatus and method for separating magnetic and/or magnetically-labeled particles from a test medium. Test medium within a reaction chamber is caused to flow past a collecting surface, and a high-gradient magnetic field is applied to the surface to capture magnetically responsive particles in the test medium. The particles are deflected toward the collection surface by baffles, a spinner, or a sprayer, or are funneled past the surface by a plunger operable to be displaced into close proximity to the surface to provide a narrow flow path for the particle-laden test medium. The particles normally suspended in the medium are separated out of suspension by adhesion to the collection surface. The particles may be resuspended by removal of the surface from the high-gradient field, or removal of the high-gradient field from the surface. The collection surface is a thin-walled non-magnetic material having a plurality of magnetic pole faces positioned therearound.
    Type: Grant
    Filed: February 6, 2002
    Date of Patent: June 6, 2006
    Assignee: ImmuniVest Corporation
    Inventors: Leon W.M.M. Terstappen, Gerald V. Doyle, Paul A. Liberti, Gerald J. Dolan
  • Patent number: 7052917
    Abstract: The present invention is to prepare a novel polymerizable biotin derivative represented by formula (I) shown below and make it possible to synthesize or design a polyfunctional or multifunctional polymer using the derivative. In formula (I), R2 represents a hydrogen atom or an alkyl group, R3 and R4 each independently represents a hydrogen atom, an alkyl group or an aryl group, T represents an oxygen atom or ?NH, W represents a single bond, a carbonyl group, a thiocarbonyl group or a C1-5 alkylene group, U represents a single bond or —NH—, X represents a single bond, a C1-8 hydrocarbon bond, an oxygen atom or —NH—, Y represents a single bond, a carbonyl group, a thiocarbonyl group, —NH—, a 1,2-dioxyethylene group or a 1,2-diaminoethylene group, Z represents a single bond, a carbonyl group, a thiocarbonyl group, a C1-5 alkylene group, an oxygen atom or —NH—, and V represents a single bond or a C1-5 alkylene group.
    Type: Grant
    Filed: July 28, 2000
    Date of Patent: May 30, 2006
    Assignees: National Institute of Advanced Industrial Science and Technology, Japan Chemical Innovation Institute
    Inventors: Noriyuki Ohnishi, Mikiko Yoshida, Kazunori Kataoka, Katsuhiko Ueno
  • Patent number: 7049152
    Abstract: The present invention herein provides the design, synthesis and characterization of compositions comprising asymmetric bolaamphiphilic lipids that form extended polymeric ribbons and wide sheets. These compositions may be doped, or interspersed, with various compounds to fine-tune the fluidity and rigidity of the bolaamphiphilic lipid composition, and promote other morphologies of the composition, including fluid vesicles and truncated flat sheets. Upon an increase in pH these compositions undergo a calorimetric and morphological transformation.
    Type: Grant
    Filed: March 12, 2002
    Date of Patent: May 23, 2006
    Assignee: The Regents of the University of California
    Inventors: Raymond C. Stevens, Quan Cheng, Jie Song
  • Patent number: 7049130
    Abstract: An improved multi-layered diagnostic sanitary test strip for receiving a heterogenous fluid, such as whole blood, to test for presence and/or amount of a suspected analyte in the fluid by facilitating a color change in the strip corresponding to the amount of the analyte in the fluid, wherein the test strip includes fluid volume control dams to prevent spillage of the fluid from the strip and a chemical reagent solution that facilitates end-point testing.
    Type: Grant
    Filed: March 21, 2005
    Date of Patent: May 23, 2006
    Assignee: Home Diagnostics, Inc.
    Inventors: Patrick Carroll, Jon Schneider, Douglas E. Bell